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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 253 records · Page 14

Free convection about a rectangular prismatic crystal growing from a solution

Sodium chlorate crystals growing from unstirred aqueous solutions were observed by Schlieren techniques. Growth rate data were best correlated by a Sherwood number with finite interface kinetics and a characteristic length equal to the crystal height plus the inverse reciprocal sum of the horizontal dimensions. The growth rate was slightly higher when the plume was laminar than when it was irregular. The horizontal growth rate was greater than the vertical growth rate, with the ratio tending to decrease with increasing plume instability. The threshold Grashof number for plume instability was greatly decreased with decreasing height for crystals with the same horizontal dimensions. The velocity of the eddies approached a constant value with increasing distance above the crystal surface, the terminal value being roughly proportional to the ratio of the square root of the Rayleigh number to the crystal characteristic length.

Chen, P.-S.↗

A study of some approximations in the modeling of a coupled rotor/fuselage aeromechanical system

Equations of motion for a three-bladed hingeless rotor coupled to a gimbal-supported fuselage are developed. A brief derivation of the equations is presented, including the assumptions made, the ordering scheme, and a discussion of both the source of the aerodynamic axial load term and the reason for differences due to hinge sequence. The solution of the equations is discussed, including linearization, the use of multiblade coordinates to eliminate periodic coefficients, and specialization to a root-sprung model. The treatment of the blade root offset by using an ordering scheme to neglect higher-order terms is found to affect results in pitch and roll. A small aerodynamic loading term in the blade axial direction has a significant effect at large collective pitch settings, as does the choice of a flap-lag or a lag-flap transformation sequence for blade motion. The results for a hingeless rotor also differ significantly from those for the root-sprung model when the nonrotating natural frequencies are matched.

Blelloch, P. A.↗

The excitation of plasma waves by a current source moving in a magnetized plasma - Two-dimensional propagation

The steady motion of a large conducting body in a magnetized plasma has been shown to create a spatial structure of charges and currents in the plasma which propagate without attenuation along the magnetic field direction (the label 'Alfven wings' has been given to this structure because of its resemblance to the wings of a biplane). It has been found that the Alfven wing structure breaks up into individual Fourier components which propagate away from the source at an angle slightly greater with respect to the magnetic field than the alignment of the original structure. This process depends on wave frequency. A given frequency is lost from the Alfven wing structure at a distance which varies as 1/omega-cubed. The amplitude of these traveling waves decreases at a rate which is inversely proportional to the square root of the distance from the source.

Rasmussen, Craig E.↗

LISA technology department at JPL

The Laser Interferometer Space Antenna (LISA) project requires technology developments on many fronts. A physical understanding of the LISA subsystems is a precursor to tacjling the requirements needed to ensure a successful mission. This can only be achieved by developing the concepts in the lab prior to developing the payload. This poster presents updates on laboratory activities intended to prove the feasibility of measuring proof mass back reaction forces of less than 3fN/(square root)Hz at 1 mHz; the sensing of the proof mass position with in 2 nm/(square root)above the 3 mHz; and the resolution and accuracy of the phase-meter to better than 10 pm/(square root)Hz above the 3 mHz.

optical displacement sensor↗

Effects of root litter traits on soil organic matter dynamics depend on decay stage and root branching order

Decaying roots contribute disproportionately to soil organic carbon (SOC) stocks in many ecosystems, yet few studies have examined the afterlife effects of root trait variability on the formation, stability and mineralization of SOC. In order to assess how root functional class (absorptive vs. transport) and mycorrhizal association (ectomycorrhizal vs. arbuscular mycorrhizal fungi) affect both formation and loss of SOC, we decayed lower-order absorptive (orders 1–2) and higher-order transport (orders 3–4) roots from six arbuscular mycorrhizal (AM) and six ectomycorrhizal (EcM) associated hardwood tree species in an isotopically distinct soil under laboratory conditions. We found that root litter effects on SOC differed significantly among root functional classes and mycorrhizal types. AM absorptive and transport roots (high in nitrogen and soluble compounds) contributed more to the formation of new mineral-associated organic carbon (MAOC), but also contributed to higher mineralization of native SOC (via priming effects). Moreover, absorptive AM roots formed MAOC gradually and rates saturated, whereas ECM roots formed MAOC more quickly, despite slower decay (via greater formation efficiency). Altogether, these findings demonstrate how different combinations of root traits might contribute to soil C balance, and that failure to account for the chemical and morphological heterogeneity of roots, as well their interactions with microbes over time, may lead to incorrect projections of SOC stocks and turnover.

59 BASIC BIOLOGICAL SCIENCES↗

A Spaceborne Demonstration of P-Band Signals-of-Opportunity (SoOp) Reflectometry

Land-reflected signals from a geosynchronous communication satellite broadcasting in P-band (367.5 MHz) were captured in low Earth orbit (LEO) using a simple dipole antenna. A delay-Doppler map (DDM) was generated through autocorrelation. Estimates of the specular point delay were obtained from the lag of the second peak in the DDM with a bias of 239.4 m and a standard deviation of 44 m (12 m over a frozen lake) with respect to a predicted orbit model. Relative magnitudes of the first and second DDM peaks fell within the range of values predicted using dielectric models for the frozen ground and lake. Finally, retrievals of surface reflection coefficient were generated using a range of realistic values for the transmitter link budget G/T , and these also fell within the range of possible values for the antenna gain pattern. Given the lack of calibration and the large uncertainties in the receiver orbit and attitude, this agreement is sufficient to conclude a successful demonstration of the fundamental principle of single-antenna reflectometry in P-band. P-band reflectometry may offer a new approach to remote sensing of sub-canopy and root-zone soil moisture.

Delays↗

Getting to the Root of Plant–Mediated Methane Emissions and Oxidation in a Thermokarst Bog

Vascular plants are important in the wetland methane cycle, but their effect on production, oxidation, and transport has high uncertainty, limiting our ability to predict emissions. In a permafrost–thaw bog in Interior Alaska, we used plant manipulation treatments, field–deployed planar optical oxygen sensors, direct measurements of methane oxidation, and microbial DNA analyses to disentangle mechanisms by which vascular vegetation affect methane emissions. Vegetation operated on top of baseline methane emissions, which varied with proximity to the thawing permafrost margin. Emissions from vegetated plots increased over the season, resulting in cumulative seasonal methane emissions that were 4.1–5.2 g m –2 season –1 greater than unvegetated plots. Mass balance calculations signify these greater emissions were due to increased methane production (3.0–3.5 g m –2 season –1 ) and decreased methane oxidation (1.1–1.6 g m –2 season –1 ). Minimal oxidation occurred along the plant–transport pathway, and oxidation was suppressed outside the plant pathway. Our data indicate suppression of methane oxidation was stimulated by root exudates fueling competition among microbes for electron acceptors. This contention is supported by the fact that methane oxidation and relative abundance of methanotrophs decreased over the season in the presence of vegetation, but methane oxidation remained steady in unvegetated treatments; oxygen was not detected around plant roots but was detected around silicone tubes mimicking aerenchyma; and oxygen injection experiments suggested that oxygen consumption was faster in the presence of vascular vegetation. In conclusion, root exudates are known to fuel methane production, and our work provides evidence they also decrease methane oxidation.

58 GEOSCIENCES↗

Dual-mycorrhizal colonization is determined by plant age and host identity in two species of Populus

Plants have evolved symbioses with mycorrhizal and endophytic fungi that are essential for their growth and survival. While most plants associate with a single guild of mycorrhizal fungi, a select group termed “dual-mycorrhizal plants” associate with both arbuscular mycorrhizal and ectomycorrhizal fungi. Although a shift from predominance of arbuscular mycorrhizal to ectomycorrhizal colonization with plant development has been demonstrated on other dual-mycorrhizal hosts, it is not known how mycorrhizal colonization shifts with plant age in Populus species. We performed a controlled growth experiment with natural field-sourced inocula to test for age-dependent shifts in fungal colonization rates and for host-specific patterns of colonization in two species of Populus (P. tremuloides and P. trichocarpa). We found that only P. trichocarpa displayed dual-mycorrhizal colonization, while P. tremuloides associated with ectomycorrhizal fungi, but not arbuscular mycorrhizal fungi. Both guilds of mycorrhizal fungi increased in abundance with plant age, while root endophytic fungal colonization decreased. Many of the early-colonizing endophytic fungi that we documented have strong saprotrophic capabilities, which may be an important trait for fast colonization. Dark septate endophytes were more abundant than either guild of mycorrhizal fungi, and are likely to be functionally important members of the Populus root fungal community. Our findings represent a novel pattern in the development of dual-mycorrhizal colonization and illustrate that Populus species vary in their association with arbuscular mycorrhizal fungi. In conclusion, our results also highlight the importance of dark septate endophyte colonization dynamics on dual-mycorrhizal plants.

59 BASIC BIOLOGICAL SCIENCES↗

Multi-objective optimization of root phenotypes for nutrient capture using evolutionary algorithms

Root phenotypes are avenues to the development of crop cultivars with improved nutrient capture, which is an important goal for global agriculture. The fitness landscape of root phenotypes is highly complex and multidimensional. It is difficult to predict which combinations of traits (phene states) will create the best performing integrated phenotypes in various environments. Brute force methods to map the fitness landscape by simulating millions of phenotypes in multiple environments are computationally challenging. Evolutionary optimization algorithms may provide more efficient avenues to explore high dimensional domains such as the root phenotypic space. We coupled the three-dimensional functional–structural plant model, SimRoot, to the Borg Multi-Objective Evolutionary Algorithm (MOEA) and the evolutionary search over several generations facilitated the identification of optimal root phenotypes balancing trade-offs across nutrient uptake, biomass accumulation, and root carbon costs in environments varying in nutrient availability. Our results show that several combinations of root phenes generate optimal integrated phenotypes where performance in one objective comes at the cost of reduced performance in one or more of the remaining objectives, and such combinations differed for mobile and non-mobile nutrients and for maize (a monocot) and bean (a dicot). Functional–structural plant models can be used with multi-objective optimization to identify optimal root phenotypes under various environments, including future climate scenarios, which will be useful in developing the more resilient, efficient crops urgently needed in global agriculture.

59 BASIC BIOLOGICAL SCIENCES↗

AmeriFlux FLUXNET-1F US-Dk1 Duke Forest-open field

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-Dk1 Duke Forest-open field. This is the FLUXNET version of the carbon flux data for the site US-Dk1 Duke Forest-open field produced by applying the standard ONEFlux (1F) software. Site Description - The Duke Forest grass field is approximately 480×305 m, dominated by the C3 grass Festuca arundinacea Shreb. (tall fescue) includes minor components of C3 herbs and the C4 grass Schizachyrium scoparium (Michx.) Nash, not considered here. The site was burned in 1979 and is mowed annually during the summer for hay according to local practices. Lai, C.T. and G.G. Katul, 2000, "The dynamic role of root-water uptake in coupling potential to actual transpiration" , Advances in Water Resources, 23, 427-439; Novick , K.A., P. C. Stoy, G. G. Katul, D. S. Ellsworth, M. B. S. Siqueira, J. Juang, R. Oren, 2004, Carbon dioxide and water vapor exchange in a warm temperate grassland, Oecologia, 138, 259-274; Stoy PC, Katul GG, Siqueira MBS, Juang J-Y, McCarthy HR, Oishi AC, Uebelherr JM, Kim H-S, Oren R (2006). Separating the effects of climate and vegetation on evapotranspiration along a successional chronosequence in the southeastern U.S. Global Change Biology 12:2115-2135

Oishi, Chris [USDA Forest Service]↗

Deterministic properties of the recursive separable median filter

The recursive separable median filter has been successfully used to extract features from noisy two-dimensional signals. In many applications, it gives better noise suppression and edge preservation than the standard separable median filter. In this paper, a new approach is used for studying the deterministic properties of separable median filters. In particular, using threshold decomposition, the root structure of the recursive separable median filter is derived, where a root is a signal invariant to further filtering. It is shown that these root structures differ from those of their nonrecursive counterparts. It is also shown that any two-dimensional signal will converge to a root after repeated passes of the recursive separable median filter.

Mcloughlin, Michael P.↗

How roots perceive and respond to gravity

Graviperception by plant roots is believed to occur via the sedimentation of amyloplasts in columella cells of the root cap. This physical stimulus results in an accumulation of calcium on the lower side of the cap, which in turn induces gravicurvature. In this paper we present a model for root gravitropism integrating gravity-induced changes in electrical potential, cytochemical localization of calcium in cells of gravistimulated roots, and the interdependence of calcium and auxin movement. Key features of the model are that 1) gravity-induced redistribution of calcium is an early event in the transduction mechanism, and 2) apoplastic movement of calcium through the root-cap mucilage may be an important component of the pathway for calcium movement.

Non-NASA Center↗

Root cytoskeleton: its role in perception of and response to gravity

We have critically evaluated the possible functions of the plant cytoskeleton in root gravisensing and graviresponse and discussed the evidence that microtubules (MTs) and actin microfilaments (MFs) do not control differential cell growth during bending of roots. On the other hand, MF and MT networks are envisaged to participate in gravisensing because of the mechanical properties of the cytoskeletal structures that interconnect plant cell organelles with the plasma membrane. In restrained gravisensing, forces are suggested to be transmitted to membranes because large-scale gravity-dependent repositioning of organelles is effectively prevented due to the cytoskeleton-mediated anchorage of their envelopes at the plasma membrane. From the cytoskeletal point of view, we can also envisage an unrestrained gravity sensing when cytoskeletal tethers are not strong enough to preserve the tight control over distribution of organelles and the latter, if heavy enough, are allowed to sediment towards the physical bottom of cells. This situation obviously occurs in root cap statocytes because these uniquely organized cells are depleted of prominent actin MF bundles, endoplasmic MT arrays, and ER elements in their internal cytoplasm. Nevertheless, indirect evidence clearly indicates that sedimented root cap statoliths are enmeshed within fine but dynamic MF networks and that their behaviour is obviously under, at least partial, cytoskeletal control. The actomyosin-enriched domain among and around amyloplasts is proposed to increase the perception of gravity due to the grouping effect of sedimenting statoliths. Cytoskeletal links between myosin-rich statoliths, and cell peripheries well equipped with dense cortical MTs, membrane-associated cytoskeleton, as well as with ER elements, would allow efficient restrained gravisensing only at the statocyte cell cortex. As a consequence of cytoskeletal depletion in the internal statocyte cytoplasm and bulk sedimentation of large amyloplasts, restrained gravisensing is spatially restricted to the bottom of the statocyte irrespective of whether roots are vertical or horizontal. This spatial aspect allows for efficient gravisensing via amplification of gravity-induced impacts on the cellular architecture, a phenomenon which is unique to root cap statocytes.

NASA Discipline Plant Biology↗

Space stress and genome shock in developing plant cells

In the present paper I review symptoms of stress at the level of the nucleus in cells of plants grown in space under nonoptimized conditions. It remains to be disclosed to what extent gravity "unloading" in the space environment directly contributes to the low mitotic index and the chromosomal anomalies and damage that is frequently, but not invariably, demonstrable in space-grown plants. Evaluation of the available facts indicates that indirect effects play a major role and that there is a significant biological component to the susceptibility to stress damage equation as well. Much remains to be learned on how to provide strictly controlled, optimal environments for plant growth in space. Only after optimized controls become possible will one be able to attribute any observed space effects to lowered gravity or to other significant but more indirect effects of the space environment.

Review, Tutorial↗

Cytological and ultrastructural studies on root tissues

The anatomy and fine structure of roots from oat and mung bean seedlings, grown under microgravity conditions for 8 days aboard the Space Shuttle, was examined and compared to that of roots from ground control plants grown under similar conditions. Roots from both sets of oat seedlings exhibited characteristic monocotyledonous tissue organization and normal ultrastructural features, except for cortex cell mitochondria, which exhibited a 'swollen' morphology. Various stages of cell division were observed in the meristematic tissues of oat roots. Ground control and flight-grown mung bean roots also showed normal tissue organization, but root cap cells in the flight-grown roots were collapsed and degraded in appearance, especially at the cap periphery. At the ultrastructural level, these cells exhibited a loss of organelle integrity and a highly-condensed cytoplasm. This latter observation perhaps suggests a differing tissue sensitivity for the two species to growth conditions employed in space flight. The basis for abnormal root cap cell development is not understood, but the loss of these putative gravity-sensing cells holds potential significance for long term plant growth orientation during space flight.

NASA Program Space Biology↗