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Lipid Biomarkers for a Hypersaline Microbial Mat Community

The use of lipid biomarkers and their carbon isotopic compositions are valuable tools for establishing links to ancient microbial ecosystems. As witnessed by the stromatolite record, benthic microbial mats grew in shallow water lagoonal environments where microorganisms had virtually no competition apart from the harsh conditions of hypersalinity, desiccation and intense light. Today, the modern counterparts of these microbial ecosystems find appropriate niches in only a few places where extremes eliminate eukaryotic grazers. Answers to many outstanding questions about the evolution of microorganisms and their environments on early Earth are best answered through study of these extant analogs. Lipids associated with various groups of bacteria can be valuable biomarkers for identification of specific groups of microorganisms both in ancient organic-rich sedimentary rocks (geolipids) and contemporary microbial communities (membrane lipids). Use of compound specific isotope analysis adds additional refinement to the identification of biomarker source, so that it is possible to take advantage of the 3C-depletions associated with various functional groups of organisms (i.e. autotrophs, heterotrophs, methanotrophs, methanogens) responsible for the cycling of carbon within a microbial community. Our recent work has focused on a set of hypersaline evaporation ponds at Guerrero Negro, Baja California Sur, Mexico which support the abundant growth of Microcoleus-dominated microbial mats. Specific biomarkers for diatoms, cyanobacteria, archaea, green nonsulfur (GNS), sulfate reducing, and methanotrophic bacteria have been identified. Analyses of the ester-bound fatty acids indicate a highly diverse microbial community, dominated by photosynthetic organisms at the surface.

Jahnke, Linda L.↗

Microfossils in cherts from the Middle Riphean (Mesoproterozoic) Avzyan Formation, southern Ural Mountains, Russian Federation

A diverse assemblage of well-preserved microorganisms has been detected in black cherts from the approximately 1200 Ma-old Avzyan Formation (Suite) of the southern Ural Mountains, Russian Federation. The lower Kataskin Member contains a diverse, abundant microbiota dominated by mat-forming filamentous cyanobacteria, several types of colonial unicells, and morphologically distinctive stalked cyanobacteria. The upper Revet Member contains a less diverse biota dominated by unicellular cyanobacteria. Palaeoecological evidence indicates that the microbial community of the Kataskin Member inhabited a shallow water, presumably marine, carbonate environment. Revet microorganisms possibly lived in restricted peritidal environments. The biostratigraphic significance of the Avzyan microbiota is limited. Many of the taxa are long-ranging; they were already abundant in Palaeoproterozoic successions and continue into the Neoproterozoic. Nevertheless, in many respects, the Kataskin assemblage is comparable to those reported from the Middle-Late Riphean deposits of Northern America, Australia and Eurasia. The following taxa are here described: Chroococcaceae-Eogloeocapsa avzyanica Sergeev, Gloeodiniopsis lamellosa Schopf emend. Knoll et Golubic; Entophysalidaceae-Eoentophysalis belcherensis Hofmann; Dermocarpaceae-Polybessurus bipartitus Fairchild ex Green et al.; Nostocaceae-Eosphaeronostoc kataskinicum Sergeev; Nostocaceae or Oscillatoriaceae-Siphonophycus robustum (Schopf) emend. Knoll et Golubic emend. Knoll et al., Siphonophycus sp.; Incertae sedis-Eosynechococcus amadeus Knoll et Golubic.

Non-NASA Center↗

Microfossils in cherts from the Mesoproterozoic (Middle Riphean) Debengda Formation, the Olenek Uplift, northeastern Siberia

Silicified shallow-water marine carbonate deposits of the Proterozoic Debengda Formation (the Olenek Uplift, northeastern Siberia) contain well preserved microfossils. One or two distinct assemblages consists only of filamentous Siphonophycus microfossils, which are presumably the extracellular sheaths of hormogonium cyanobacteria. The other is dominated by coccoidal microfossils, first by the entophysalidacean cyanobacterium Eoentophysalis. The coccoidal assemblage was recognized in the layered carbonate precipitate structures of a superficially stromatolite appearance. Despite its simple composition, the microfossil assemblage supports the generally accepted Mesoproterozoic (middle Riphean) age of the Debengda Formation. This conclusion corresponds to the available data on isotopic geochronology, and to the composition of columnar stromatolites from the Dehengda Formation. Both the structural features and carbon isotopic composition of its rocks are comparable to those of rocks of known Mesoproterozoic age, but differ from the characteristics of definitely Neoproterozoic deposits.

Non-NASA Center↗

Shewanella amazonensis sp. nov., a novel metal-reducing facultative anaerobe from Amazonian shelf muds

A new bacterial species belonging to the genus Shewanella is described on the basis of phenotypic characterization and sequence analysis of its 16S rRNA-encoding and gyrase B (gyrB) genes. This organism, isolated from shallow-water marine sediments derived from the Amazon River delta, is a Gram-negative, motile, polarly flagellated, facultatively anaerobic, rod-shaped eubacterium and has a G&C content of 51.7 mol%. Strain SB2BT is exceptionally active in the anaerobic reduction of iron, manganese and sulfur compounds. SB2BT grows optimally at 35 degrees C, with 1-3% NaCl and over a pH range of 7-8. Analysis of the 16S rDNA sequence revealed a clear affiliation between strain SB2BT and members of the gamma subclass of the class Proteobacteria. High similarity values were found with certain members of the genus Shewanella, especially with Shewanella putrefaciens, and this was supported by cellular fatty acid profiles and phenotypic characterization. DNA-DNA hybridization between strain SB2BT and its phylogenetically closest relatives revealed low similarity values (24.6-42.7%) which indicated species status for strain SB2BT. That SB2BT represents a distinct bacterial species within the genus Shewanella is also supported by gyrB sequence analysis. Considering the source of the isolate, the name Shewanella amazonensis sp. nov. is proposed and strain SB2BT (= ATCC 700329T) is designated as the type strain.

NASA Discipline Exobiology↗

High-Frequency Orographically Forced Variability in a Single-Layer Model of the Martian Atmosphere

A shallow water model with realistic topography and idealized zonal wind forcing is used toinvestigate orographically forced modes in the Martian atmosphere. Locally, the model reproduceswell the climatology at the sites of Viking Lander I and II (VL1 and VL2) as inferred from theViking Lander fall and spring observations. Its variability at those sites is dominated by a 3-sol(Martian solar day) oscillation in the region of VL1 and by a 6-sol oscillation in that of VL2. Theseoscillations are forced by the zonal asymmetries of the Martian mountain field. It is suggested thatthey contribute to the observed variability by reinforcing the baroclinic oscillations with nearbyperiods identified in observational studies. The spatial variability associated with the orographicallyforced oscillations is studied by means of extended empirical orthogonal function analysis. The 3-solVL1 oscillation corresponds to a tropical, eastward-traveling, zonal-wavenumber one pattern...

Keppenne, C. L.↗

Fibonacci Grids

Recent years have seen a resurgence of interest in a variety of non-standard computational grids for global numerical prediction. The motivation has been to reduce problems associated with the converging meridians and the polar singularities of conventional regular latitude-longitude grids. A further impetus has come from the adoption of massively parallel computers, for which it is necessary to distribute work equitably across the processors; this is more practicable for some non-standard grids. Desirable attributes of a grid for high-order spatial finite differencing are: (i) geometrical regularity; (ii) a homogeneous and approximately isotropic spatial resolution; (iii) a low proportion of the grid points where the numerical procedures require special customization (such as near coordinate singularities or grid edges). One family of grid arrangements which, to our knowledge, has never before been applied to numerical weather prediction, but which appears to offer several technical advantages, are what we shall refer to as "Fibonacci grids". They can be thought of as mathematically ideal generalizations of the patterns occurring naturally in the spiral arrangements of seeds and fruit found in sunflower heads and pineapples (to give two of the many botanical examples). These grids possess virtually uniform and highly isotropic resolution, with an equal area for each grid point. There are only two compact singular regions on a sphere that require customized numerics. We demonstrate the practicality of these grids in shallow water simulations, and discuss the prospects for efficiently using these frameworks in three-dimensional semi-implicit and semi-Lagrangian weather prediction or climate models.

Swinbank, Richard↗

2005 AG20/20 Annual Review

Topics covered include: Implementation and Validation of Sensor-Based Site-Specific Crop Management; Enhanced Management of Agricultural Perennial Systems (EMAPS) Using GIS and Remote Sensing; Validation and Application of Geospatial Information for Early Identification of Stress in Wheat; Adapting and Validating Precision Technologies for Cotton Production in the Mid-Southern United States - 2004 Progress Report; Development of a System to Automatically Geo-Rectify Images; Economics of Precision Agriculture Technologies in Cotton Production-AG 2020 Prescription Farming Automation Algorithms; Field Testing a Sensor-Based Applicator for Nitrogen and Phosphorus Application; Early Detection of Citrus Diseases Using Machine Vision and DGPS; Remote Sensing of Citrus Tree Stress Levels and Factors; Spectral-based Nitrogen Sensing for Citrus; Characterization of Tree Canopies; In-field Sensing of Shallow Water Tables and Hydromorphic Soils with an Electromagnetic Induction Profiler; Maintaining the Competitiveness of Tree Fruit Production Through Precision Agriculture; Modeling and Visualizing Terrain and Remote Sensing Data for Research and Education in Precision Agriculture; Thematic Soil Mapping and Crop-Based Strategies for Site-Specific Management; and Crop-Based Strategies for Site-Specific Management.

Ross, Kenton W.↗

A Finite Volume Scheme on the Cubed Sphere Grid

The performance of a multidimensional finite-volume scheme for global atmospheric dynamics is evaluated on the cubed-sphere geometry. We will explore the properties of the finite volume scheme through traditional advection and shallow water test cases. Baroclinic evaluations performed via a recently developed deterministic initial value baroclinic test case from Jablonowski and Williamson that assesses the evolution of an idealized baroclinic wave in the Northern Hemisphere for a global 3-dimensional atmospheric dynamical core. Comparisons will be made when available to the traditional latitude longitude discretization of the finite-volume dynamical core, as well as other traditional gridpoint and spectral formulations for atmospheric dynamical cores.

Putman, William M.↗

Organic Geochemistry of the Hamersley Province: Relationships Among Organic Carbon Isotopes, Molecular Fossils, and Lithology

Molecular fossils are particularly valuable ancient biosignatures that can provide key insight about microbial sources and ecology in early Earth studies. In particular, hopanes carrying 2-methyl or 3-methyl substituents are proposed to be derived from cyanobacteria and oxygen-respiring methanotrophs, respectively, based on both their modem occurrences and their Proterozoic and Phanerozoic sedimentary distributions. Steranes are likely from ancestral eukaryotes. The distribution of methylhopanes, steranes, and other biomarkers in 2.72-2.56 billion-year-old rocks from the Hamersley Province, Western Australia show relationships to lithology, facies, and isotopes of macromolecular carbon, and other biomarkers. These observations support biomarker syngenicity and thermal maturity. Moreover, ecological signatures are revealed, including a surprising relationship between isotopic values for bulk macromolecular carbon and the biomarker for methanotrophs. The record suggests that cyanobacteria were likely key organisms of shallow-water microbial ecosystems providing molecular oxygen, fixed carbon, and possibly fixed nitrogen, and methanotrophs were not alone in recycling methane and other C-13-depleted substrates.

Eigenbrode, Jennifer L.↗

POSTMAN: Point of Sail Tacking for Maritime Autonomous Navigation

Waves apply significant forces to small boats, in particular when such vessels are moving at a high speed in severe sea conditions. In addition, small high-speed boats run the risk of diving with the bow into the next wave crest during operations in the wavelengths and wave speeds that are typical for shallow water. In order to mitigate the issues of autonomous navigation in rough water, a hybrid controller called POSTMAN combines the concept of POS (point of sail) tack planning from the sailing domain with a standard PID (proportional-integral-derivative) controller that implements reliable target reaching for the motorized small boat control task. This is an embedded, adaptive software controller that uses look-ahead sensing in a closed loop method to perform path planning for safer navigation in rough waters. State-of-the-art controllers for small boats are based on complex models of the vessel's kinematics and dynamics. They enable the vessel to follow preplanned paths accurately and can theoretically control all of the small boat s six degrees of freedom. However, the problems of bow diving and other undesirable incidents are not addressed, and it is questionable if a six-DOF controller with basically a single actuator is possible at all. POSTMAN builds an adaptive capability into the controller based on sensed wave characteristics. This software will bring a muchneeded capability to unmanned small boats moving at high speeds. Previously, this class of boat was limited to wave heights of less than one meter in the sea states in which it could operate. POSTMAN is a major advance in autonomous safety for small maritime craft.

Huntsberger, Terrance L.↗

Atmospheric Waves and Dynamics Beneath Jupiters Clouds from Radio Wavelength Observations

We observed Jupiter at wavelengths near 2 cm with the Karl G. Jansky Very Large Array (VLA) in February 2015. These frequencies are mostly sensitive to variations in ammonia abundance and probe between approx. 0.5- 2.0 bars of pressure in Jupiters atmosphere; within and below the visible cloud deck which has its base near 0.7 bars. The resultant observed data were projected into a cylindrical map of the planet with spatial resolution of approx. 1500 km at the equator. We have examined the data for atmospheric waves and observed a prominent bright belt of radio hotspot features near 10 N, likely connected to the same equatorial wave associated with the 5-m hotspots. We conducted a passive tracer power spectral wave analysis for the entire map and latitude regions corresponding to eastward and westward jets and compare our results to previous studies. The power spectra analysis revealed that the atmosphere sampled in our observation (excluding the NEB region) is in a 2-D turbulent regime and its dynamics are predominately governed by the shallow water equations. The Great Red Spot (GRS) is also very prominent and has a noticeable meridional asymmetry and we compare it, and nearby storms, with optical images. We find that the meridional radio profile has a global north-south hemisphere distinction and find correlations of it to optical intensity banding and to shear zones of the zonal wind profile over select regions of latitude. Amateur optical images taken before and after our observation complemented the radio wave- length map to investigate dynamics of the equatorial region in Jupiters atmosphere. We find that two radio hotspots at 2 cm are well correlated with optical plumes in the NEB, additionally revealing they are not the same 5 m hotspot features correlated with optical dark patches between adjacent plumes. This analysis exploits the VLAs upgraded sensitivity and explores the opportunities now possible when studying gas giants, especially atmospheric dynamics of layers beneath upper level clouds.

radio observations↗

Use of Multi-Spectral High Repetition Rate LED Systems for High Bandwidth Underwater Optical Communications, and Communications to Surface and Aerial Systems

A variety of both existing and developing sensors would benefit from near real time communication of high bandwidth data. To cite just one example, sensors that could more accurately report real-time positions of marine mammals would be useful in reducing whale-ship collisions. Similar considerations are relevant for maritime port and harbor security, including detection and alerts for divers or autonomous underwater vehicles (AUVs) that could pose a risk to ships. Especially in ports and harbors, field experiments have confirmed that acoustic communication in these cluttered and noisy shallow water environments, compounded with vertical reflecting surfaces formed by piers and pilings, can limit the reliability and utility of underwater acoustic communications. Moreover, many sensors have greater bandwidth requirements than acoustic communications are able to provide. We here discuss the development of high repetition rate multispectral LED optical systems initially developed for imaging, but also capable of simultaneous data transmission at rates of approximately100 kilobits per second. Results are discussed for the multispectral images from coral reefs in Guam, and data transmission experiments from underwater to surface vessels. Subsequent field efforts will extend data transmission from AUVs to unmanned aircraft systems (UAS).

Underwater↗

The Don Edwards San Francisco Bay National Wildlife Refuge: A Natural Laboratory for Federal Agencies and Partners

The Don Edwards San Francisco Bay National Wildlife Refuge encompasses a rich mosaic of habitats and is a fecund and convenient field site for Earth science research, comprised of tidal salt marsh, open ponds, shallow water, mudflat habitat and encompassing a variety of tide, salinity, elevation, slope, and other conditions. Proximity to the NASA Ames Research Center and overlap with the center's UAS (unmanned aircraft systems) test area presents the opportunity to create a unique natural laboratory that can incubate and support a variety of research and benefit the Refuge in monitoring wildlife changes and habitat evolution over time.

Unmanned Airborne Vehicle↗

On the Evolution-Dynamics of Tropical Ocean-Atmosphere Annual-Cycle Variability

The structure of ocean-atmosphere annual-cycle variability across the global tropics is extracted from the Comprehensive Ocean-Atmosphere Data Set (COADS) surface winds and SSTs, and oceanic heat-content simulation from a nonlinear shallow water model (forced by COADS wind stress) using the co-variance based rotated principal component analysis technique.

COADS↗

Forcing Factors Affecting Sea Level Changes at the Coast

We review the characteristics of sea level variability at the coast focussing on how it differs from the variability in the nearby deep ocean. Sea level variability occurs on all timescales, with processes at higher frequencies tending to have a larger magnitude at the coast due to resonance and other dynamics. In the case of some processes, such as the tides, the presence of the coast and the shallow waters of the shelves results in the processes being considerably more complex than offshore. However, ‘coastal variability’ should not always be considered as ‘short spatial scale variability’ but can be the result of signals transmitted along the coast from 1000s km away. Fortunately, thanks to tide gauges being necessarily located at the coast, many aspects of coastal sea level variability can be claimed to be better understood than those in the deep ocean. Nevertheless, certain aspects of coastal variability remain under-researched, including how changes in some processes (e.g., wave setup, river runoff) may have contributed to the historical mean sea level records obtained from tide gauges which are now used routinely in large-scale climate research.

Philip L Woodworth↗

Forcing Factors Affecting Sea Level Changes at the Coast

We review the characteristics of sea level variability at the coast focussing on how it differs from the variability in the nearby deep ocean. Sea level variability occurs on all timescales, with processes at higher frequencies tending to have a larger magnitude at the coast due to resonance and other dynamics. In the case of some processes, such as the tides, the presence of the coast and the shallow waters of the shelves results in the processes being considerably more complex than offshore. However, ‘coastal variability’ should not always be considered as ‘short spatial scale variability’ but can be the result of signals transmitted along the coast from 1000s km away. Fortunately, thanks to tide gauges being necessar-ily located at the coast, many aspects of coastal sea level variability can be claimed to be better understood than those in the deep ocean. Nevertheless, certain aspects of coastal variability remain under-researched, including how changes in some processes (e.g., wave setup, river runoff) may have contributed to the historical mean sea level records obtained from tide gauges which are now used routinely in large-scale climate research.

Philip L. Woodworth↗

Very High-Resolution Satellite-Derived Bathymetry and Habitat Mapping Using Pleiades-1 and ICESat-2

Accurate and reliable bathymetric data are needed for a wide diversity of marine research and management applications. Satellite-derived bathymetry represents a time saving method to map large shallow waters of remote regions compared to the current costly in situ measurement techniques. This study aims to create very high-resolution (VHR) bathymetry and habitat mapping in Mayotte island waters (Indian Ocean) by fusing 0.5 m Pleiades-1 passive multispectral imagery and active ICESat-2 LiDAR bathymetry. ICESat-2 georeferenced photons were filtered to remove noise and corrected for water column refraction. The bathymetric point clouds were validated using the French naval hydrographic and oceanographic service Litto3D® dataset and then used to calibrate the multispectral image to produce a digital depth model (DDM). The latter enabled the creation of a digital albedo model used to classify benthic habitats. ICESat-2 provided bathymetry down to 15 m depth with a vertical accuracy of bathymetry estimates reaching 0.89 m. The benthic habitats map produced using the maximum likelihood supervised classification provided an overall accuracy of 96.62%. This study successfully produced a VHR DDM solely from satellite data. Digital models of higher accuracy were further discussed in the light of the recent and near-future launch of higher spectral and spatial resolution satellites.

Bathymetry↗