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A new version of the RDP (Ribosomal Database Project)

The Ribosomal Database Project (RDP-II), previously described by Maidak et al. [ Nucleic Acids Res. (1997), 25, 109-111], is now hosted by the Center for Microbial Ecology at Michigan State University. RDP-II is a curated database that offers ribosomal RNA (rRNA) nucleotide sequence data in aligned and unaligned forms, analysis services, and associated computer programs. During the past two years, data alignments have been updated and now include >9700 small subunit rRNA sequences. The recent development of an ObjectStore database will provide more rapid updating of data, better data accuracy and increased user access. RDP-II includes phylogenetically ordered alignments of rRNA sequences, derived phylogenetic trees, rRNA secondary structure diagrams, and various software programs for handling, analyzing and displaying alignments and trees. The data are available via anonymous ftp (ftp.cme.msu. edu) and WWW (http://www.cme.msu.edu/RDP). The WWW server provides ribosomal probe checking, approximate phylogenetic placement of user-submitted sequences, screening for possible chimeric rRNA sequences, automated alignment, and a suggested placement of an unknown sequence on an existing phylogenetic tree. Additional utilities also exist at RDP-II, including distance matrix, T-RFLP, and a Java-based viewer of the phylogenetic trees that can be used to create subtrees.

Non-NASA Center↗

Hazard Detection Software for Lunar Landing

The Autonomous Landing and Hazard Avoidance Technology (ALHAT) Project is developing a system for safe and precise manned lunar landing that involves novel sensors, but also specific algorithms. ALHAT has selected imaging LIDAR (light detection and ranging) as the sensing modality for onboard hazard detection because imaging LIDARs can rapidly generate direct measurements of the lunar surface elevation from high altitude. Then, starting with the LIDAR-based Hazard Detection and Avoidance (HDA) algorithm developed for Mars Landing, JPL has developed a mature set of HDA software for the manned lunar landing problem. Landing hazards exist everywhere on the Moon, and many of the more desirable landing sites are near the most hazardous terrain, so HDA is needed to autonomously and safely land payloads over much of the lunar surface. The HDA requirements used in the ALHAT project are to detect hazards that are 0.3 m tall or higher and slopes that are 5 or greater. Steep slopes, rocks, cliffs, and gullies are all hazards for landing and, by computing the local slope and roughness in an elevation map, all of these hazards can be detected. The algorithm in this innovation is used to measure slope and roughness hazards. In addition to detecting these hazards, the HDA capability also is able to find a safe landing site free of these hazards for a lunar lander with diameter .15 m over most of the lunar surface. This software includes an implementation of the HDA algorithm, software for generating simulated lunar terrain maps for testing, hazard detection performance analysis tools, and associated documentation. The HDA software has been deployed to Langley Research Center and integrated into the POST II Monte Carlo simulation environment. The high-fidelity Monte Carlo simulations determine the required ground spacing between LIDAR samples (ground sample distances) and the noise on the LIDAR range measurement. This simulation has also been used to determine the effect of viewing on hazard detection performance. The software has also been deployed to Johnson Space Center and integrated into the ALHAT real-time Hardware-in-the-Loop testbed.

Huertas, Andres↗

The Jetting Phenomena in Meshed Spur Gears

Rotorcraft gearbox transmissions are required to efficiently transfer power from the turbine engine to the main and tail rotor blades. Losses in transmission efficiency impact mission payload and aircraft range. These systems are expected to deliver high power with high gear pitch line velocities. More recently, shrouding has been employed to reduce windage power losses associated with the high gear rotational speeds. However, recent experimental results from tests conducted by the authors show the negative impact of close clearance shrouds on windage power loss, particularly at the meshed region where flow is ejected, or jetted, from the collapsing tooth spaces. A literature review was conducted to gain further insight into the phenomenon of gear mesh jetting and strategies to mitigate and control the associated losses. An analysis was conducted on windage losses in the mesh region. Test results are given for a modified shroud configuration. Finally, a discussion on observed trends follows with suggestions on future research.

windage↗

The Jetting Phenomena in Meshed Spur Gears

Rotorcraft gearbox transmissions are required to efficiently transfer power from the turbine engine to the main and tail rotor blades. Losses in transmission efficiency impact mission payload and aircraft range. These systems are expected to deliver high power with high gear pitch line velocities. More recently, shrouding has been employed to reduce windage power losses associated with the high gear rotational speeds. However, recent experimental results from tests conducted by the authors show the negative impact of close clearance shrouds on windage power loss, particularly at the meshed region where flow is ejected, or jetted, from the collapsing tooth spaces. A literature review was conducted to gain further insight into the phenomenon of gear mesh jetting and strategies to mitigate and control the associated losses. An analysis was conducted on windage losses in the mesh region. Test results are given for a modified shroud configuration. Finally, a discussion on observed trends follows with suggestions on future research.

Gear↗

Understanding the Uncertainty and Variability of Earth’s Radiation Budget Components Over the Past 3 Decades from the NASA’s GEWEX Surface Radiation Budget Release 4 Integrated Product

The NASA/GEWEX Surface Radiation Budget (SRB) project produced a 30+ year time series of 3-hourly Top-of-Atmosphere (TOA) and surface radiative flux components at a 1x1 degree resolution. This SRB Release 4 Integrated Productare derived using imager data products from the cross-calibrated from ISCCP (International Satellite Cloud Climatology Project) “HSeries” radiance, cloud and surface property data products. The ISCCP HXS product replaces ISCCP DX with a ninefold increase in pixel count (10km instead of 30km). ISCCP also provides an atmospheric temperature and moisture dataset known as nnHIRS which we use and discuss radiative flux sensitivities to in this presentation. Total solar irradiance is now variable consistent with SORCE measurements. Sea surface (SeaFlux v2.0) and land surface products (LandFlux) are utilized to achieve an radiative flux estimatesintegrated with global data sets from these GEWEX projects. The variable aerosol composition are specified using a detailed aerosol history from the Max Planck Institute Aerosol Climatology (MAC) also used for background aerosol in the ISCCP HX. Numerous other improvements were made to the SW and LW algorithms including improved cloud optical property treatments and specification of surface spectral albedo and emissivity for various surface conditions. Here we analyze the new SWand LWSRB datasets, comparing them to the previous Release 3, BSRN, GEBA and PMEL surface measurements, and ERBE and CERES satellite datasets. For surface flux validation besides ensemble comparisons, we show the variability of SRB vs surface measurements from BSRN beginning in 1992 and GEBA SW from 1983. We assess the variability of the surface flux anomalies, particularly the net fluxes and the cloud radiative effects, over the entire time period including the period containing CERES overlap. For the TOA fluxes, the anomalies of the fluxes in the early period are compared to the MEaSURES ERBE and the latter period to CERES EBAF/SYN1Deg datasets for a benchmark. Long-term changes in the surface radiation budget components and cloud radiative effects are shown and discussed relative to CERES and surface measurements. This includes a change point analysis that helps associate various changes in the ISCCP GEO/LEO configurations and other inputs to variations in the radiative budget components.

Earth Radiation Budget↗

Development of Machine Learning Algorithms to Segment and Study Images of Astromaterial Samples

Introduction: Micrometer-scale chemical analyses of chondritic meteorites and mission-returned asteroid samples can reveal details of the physical and chemical processes operating in the early solar system, including processes that gave rise to planets, moons, and minor bodies. These primitive astromaterials are comprised of chondrules, calcium- and aluminum-rich inclusions (CAI), and many other silicates, oxides, metals, sulfides, and fine-grained materials. The chemical and mineralogical complexity of these samples, vast populations of different components, and heterogeneity across mm to km scales, all limit our understanding of the origin and evolution of these materials. Here, we describe recent efforts to use machine learning techniques to automate the segmentation of chemical maps of chondritic meteorites, designed to aid studies of asteroid samples returned by spacecraft. By automating the task of segmentation it will become possible to rapidly analyze and interpret the sizes, shapes, mineralogy, chemistry, and other properties of every chondrule, calcium- and aluminum-rich inclusion (CAI) and other clast within and between asteroid samples. Sample return missions significantly accelerate and heighten the need to develop such new data analysis techniques, and associated data repositories. Techniques: Neural networks require abundant training data, i.e. images which have been segmented by a human user. We have manually segmented data available from previous petrologic and chemical work at NASA Johnson Space Center and the American Museum of Natural History [1-4]. These data were derived from energy- and wavelength-dispersive X-ray spectroscopy (EDS, WDS) mapping of samples from many chondrite groups. The Deeplabv3+ [5] neural network architecture was trained on human-labeled masks and used to create machine-labeled masks. Several different algorithms were investigated, with inputs ranging from common RGB image formats through to hyperspectral datasets, with raw data comprising greyscale maps of Mg, Ca, and Al, with or without Si, Fe, Ti for both EDS and WDS data, and extending to other elements in EDS only. Each greyscale image was paired with a binary mask for each labelled particle type. Results: The trained algorithms can segment (Fig 1), classify, and measure the dimensions of thousands of particles in chemical maps of a standard 1-inch round petrographic section in seconds to minutes, rather than many hours needed by a human. Accuracy of the algorithms varied from chondrite to chondrite and across particle types. Further results and details of the algorithms will be presented at the workshop. Future directions: Machine learning has the potential to revolutionize our understanding of complex particle populations contained within primitive astromaterial, with segmentation being a critical first step. Example applications include better understanding of particle transport, nebular reservoirs, parent body accretion, and a deeper understanding of the relationships between particle populations and bulk rock elemental and isotopic compositions. In addition to benefits that machine learning can bring to individual researchers, building a community data repository of thousands to millions of particles across hundreds of samples will open up many other possibilities. For example, with a large enough dataset it will be possible to search for exceptionally closely matching particles across disparate samples. Such a capability would enable a single CAI from OSIRISREx or Hayabusa/II samples to be matched to chondritic CAIs that exhibit near-identical size, texture, and mineralogy, down to the level of similar core phenocrysts, zonation, and rim sequences. Such comparative analyses will help to disentangle precursor chemistry, chronology, gas/dust reservoirs during heating, and accretion. Such an endeavor would be impossible without machine learning and a large community data repository of astromaterial chemical/mineralogic maps.

Machine Learning↗

Reference Intercalibration for the Climate Observing System

Reference Intercalibration is critical in supporting the construction of climate data records, which, given their necessary longevity, must consist of measurements from multiple instruments. Reference intercalibration enables placing multiple instruments on the same radiometric scale, reducing calibration-based biases in climate data records. Measurements that have characteristics of a climate benchmark make excellent in-orbit intercalibration references. Climate Absolute Radiance and Refractivity Observatory (CLARREO) Pathfinder (CPF) consists of a reflected solar (RS) spectrometer (350-2300 nm) that will take hyperspectral Earth reflectance measurements with unprecedented SI-traceable accuracy (0.3%, 1-sigma) from the International Space Station (ISS). CPF measurements will have several characteristics of climate benchmark measurements and will demonstrate its capability as a rigorous in-orbit intercalibration reference with Clouds and Earth’s Radiant Energy System (CERES) and Visible Infrared Imaging Radiometer Suite (VIIRS). The methodologies that have been developed to support CPF-CERES and CPF-VIIRS intercalibration can readily be extended to other Low Earth Orbit and Geostationary instrument targets. With its highly accurate hyperspectral observations, CPF measurements can also be used to improve the characterization of targets widely used for satellite instrument vicarious calibration including Earth land surface pseudo-invariant calibration sites, deep convective clouds, and the Moon. We will discuss the importance of climate benchmark measurement attributes for intercalibration and considerations for the associated intercalibration data analysis to support building and maintaining climate data records.

Yolanda Shea↗

Applications and Development Work for Digital Image Correlation Using High Speed Cameras

NASA, Army, and industry partners are working to advance optical measurement techniques used in highly dynamic rocket engine and missile test environments. This allows for direct component data collection during test and flight environments, significantly reduced analysis time, and associated cost savings. Digital Image Correlation (DIC) uses calibrated stereo cameras to collect three-dimensional (3-D) displacements, strains, and acceleration data by referencing a stochastic speckle pattern applied to the test object. These optical measurement techniques provide significantly improved data resulting in better performance predictions, model validation, and information about components during hot-fire tests and test flights.

Rachel Paden↗

A CAD/CAE analysis of photographic and engineering data

In the investigation of the STS 51L accident, NASA engineers were given the task of visual analysis of photographic data extracted from the tracking cameras located at the launch pad. An analysis of the rotations associated with the right Solid Rocket Booster (SRB) was also performed. The visual analysis involved pinpointing coordinates of specific areas on the photographs. The objective of the analysis on the right SRB was to duplicate the rotations provided by the SRB rate gyros and to determine the effects of the rotations on the launch configuration. To accomplish the objectives, computer aided design and engineering was employed. The solid modeler, GEOMOD, inside the Structural Dynamics Research Corp. I-DEAS package, proved invaluable. The problem areas that were encountered and the corresponding solutions that were obtained are discussed. A brief description detailing the construction of the computer generated solid model of the STS launch configuration is given. A discussion of the coordinate systems used in the analysis is provided for the purpose of positioning the model in coordinate space. The techniques and theory used in the model analysis are described.

Goza, S. Michael↗

One-Carbon Metabolism and SANS 2022 Update

Spaceflight Associated Neuro-ocular Syndrome, or SANS, affects a subset of astronauts (1, 2), and biochemical evidence has documented differences in those astronauts (3). Specifically, they had higher circulating concentrations of metabolites of the one-carbon metabolic pathway (1C), including homocysteine, and these concentrations were higher before flight (3). After ruling out many potential confounding factors in these otherwise healthy individuals (e.g., sex, kidney function, vitamin status, coffee consumption), a study of genetics was warranted. In an initial pilot effort, we documented a genetic predisposition to develop ophthalmic changes after long-duration space flight (4). That is, from a limited study of 5 single-nucleotide polymorphisms (SNPs), we found that the G allele for the MTRR A66G SNP was associated with a greater risk of choroidal folds and cotton-wool spots after flight, and the C allele for SHMT1 C1420T was protective against optic disc edema (4). These data provide a potential pathway for understanding why some individuals develop SANS, while others do not. The initial pilot study of 5 SNPs yielded striking findings, but the 1C pathway is far more complex. An effort was undertaken to examine more than 500 1C SNPs to see if a broader examination could help illuminate this association. That work is ongoing. The astronaut findings led us to advocate for the inclusion of 1C pathway genetic and biochemistry testing on other SANS-related projects, noting that genetics might help identify responders, non-responders, or outliers. The first such effort yielded evidence of an association of specific forms of the MTRR and SHMT-1 SNPs and vitamin B12 status with end-tidal CO2 after acute carbon dioxide exposure (5). The second such effort led to the identification that individuals exposed to strict head-down tilt and CO2 for 30-d who developed optic disc edema also had risk alleles for the two SNPs described above (6). Additionally, we identified a clinical population with many characteristics either attributed or purported to be involved in the ocular changes seen in affected astronauts: women with polycystic ovary syndrome (PCOS). PCOS is a condition of androgen excess and anovulatory menstrual cycles. The shared characteristics and clinical findings between SANS and PCOS generally include higher circulating homocysteine concentrations, increased retinal nerve fiber layer thickness, increased androgen concentrations (or responses), and altered carbohydrate metabolism. To our knowledge, no study has examined whether women with PCOS have asymptomatic ophthalmic anomalies observed in astronauts with SANS. While researchers have evaluated the one-carbon metabolism pathway polymorphisms of PCOS patients, and initial studies show an association with certain one-carbon polymorphisms, none have looked at the set of SNPs identified in our studies that are associated with ophthalmic changes in astronauts. Accordingly, we designed a study to evaluate the association of one-carbon pathway SNPs and ophthalmic findings in patients with PCOS and/or IIH compared to controls. Subjects provided blood samples for vitamin and one carbon biochemistry analyses, an extensive analysis of >500 SNPs associated with one carbon metabolism and had eye examinations and ocular imaging. Data analysis are underway. The data collected to date have shown associations between one carbon pathway biochemistry and genetics and incidence of SANS. The mechanisms for SANS has yet to be identified, although many hypotheses exist. Based on our data, we have developed (8, 9) and expanded (6) a multi-hit hypothesis for how these seemingly disparate findings could be linked. While intriguing, the hypothesis represents the starting point for further research. We aim to clarify the relationship between B-vitamin status and genetics with regard to the risk of SANS. Ultimately, understanding the mechanism(s) behind this will provide a means to predict, prevent, or treat these ophthalmologic pathologies in astronauts, and terrestrial populations.

S M Smith↗

Analysis of Aviation Safety Reporting System Incident Data Associated with the Technical Challenges of the Atmospheric Environment Safety Technology Project

This study analyzed aircraft incidents in the NASA Aviation Safety Reporting System (ASRS) that apply to two of the three technical challenges (TCs) in NASA's Aviation Safety Program's Atmospheric Environment Safety Technology Project. The aircraft incidents are related to airframe icing and atmospheric hazards TCs. The study reviewed incidents that listed their primary problem as weather or environment-nonweather between 1994 and 2011 for aircraft defined by Federal Aviation Regulations (FAR) Parts 121, 135, and 91. The study investigated the phases of flight, a variety of anomalies, flight conditions, and incidents by FAR part, along with other categories. The first part of the analysis focused on airframe-icing-related incidents and found 275 incidents out of 3526 weather-related incidents over the 18-yr period. The second portion of the study focused on atmospheric hazards and found 4647 incidents over the same time period. Atmospheric hazards-related incidents included a range of conditions from clear air turbulence and wake vortex, to controlled flight toward terrain, ground encounters, and incursions.

Withrow, Colleen A.↗

System Analysis Applied to Autonomy: Application to Human-Rated Lunar/Mars Landers

System analysis is an essential technical discipline for the modern design of spacecraft and their associated missions. Specifically, system analysis is a powerful aid in identifying and prioritizing the required technologies needed for mission and/or vehicle development efforts. Maturation of intelligent systems technologies, and their incorporation into spacecraft systems, are dictating the development of new analysis tools, and incorporation of such tools into existing system analysis methodologies, in order to fully capture the trade-offs of autonomy on vehicle and mission success. A "system analysis of autonomy" methodology will be outlined and applied to a set of notional human-rated lunar/Mars lander missions toward answering these questions: 1. what is the optimum level of vehicle autonomy and intelligence required? and 2. what are the specific attributes of an autonomous system implementation essential for a given surface lander mission/application in order to maximize mission success? Future human-rated lunar/Mars landers, though nominally under the control of their crew, will, nonetheless, be highly automated systems. These automated systems will range from mission/flight control functions, to vehicle health monitoring and prognostication, to life-support and other "housekeeping" functions. The optimum degree of autonomy afforded to these spacecraft systems/functions has profound implications from an exploration system architecture standpoint.

Young, Larry A.↗

Iterative solution techniques in boundary element analysis

Iterative techniques for the solution of the algebraic equations associated with the direct boundary element analysis (BEA) method are discussed. Continuum structural response analysis problems are considered, employing single- and multizone boundary element models with and without zone condensation. The impact on convergence rate and computer resource requirements associated with the sparse and blocked matrices, resulting in multizone BEA, is studied. Both conjugate gradient and generalized minimum residual preconditioned iterative solvers are applied for these problems and the performance of these algorithms is reported. Included is a quantification of the impact of the preconditioning utilized to render the boundary element matrices solvable by the respective iterative methods in a time competitive with direct methods.

Kane, J. H.↗

Experimental considerations for 2-D acoustooptic spectrum analysis

Two-dimensional acoustooptic spectrum analysis (AOSA) with the associated reference function generator and real-time image processor is presented. The resolution, bandwidth, and analysis time of the two-dimensional AOSA depend on the characteristics. The operation of the two-dimensional AOSA and the experimental considerations for obtaining a high-resolution two-dimensional spectrum in real time are described.

Ansari, Homayoon↗

Behavioral Issues Associated With Long Duration Space Expeditions: Review and Analysis of Astronaut Journals

Personal journals maintained by NASA astronauts during six-month expeditions onboard the International Space Station were analyzed to obtain information concerning a wide range of behavioral and human factors issues. Astronauts wrote most about their work, followed by outside communications (with mission control, family, and friends), adjustment to the conditions, interactions with crew mates, recreation/leisure, equipment (installation, maintenance), events (launches, docking, hurricanes, etc.), organization/management, sleep, and food. The study found evidence of a decline in morale during the third quarters of the missions and identified key factors that contribute to sustained adjustment and optimal performance during long-duration space expeditions. Astronauts reported that they benefited personally from writing in their journals because it helped maintain perspective on their work and relations with others. Responses to questions asked before, during, and after the expeditions show that living and working onboard the ISS is not as difficult as the astronauts anticipate before starting their six-month tours of duty. Recommendations include application of study results and continuation of the experiment to obtain additional data as crew size increases and operations evolve.

Struster, Jack↗

The Zwicky Transient Facility: System Overview, Performance, and First Results

The Zwicky Transient Facility (ZTF) is a new optical time-domain survey that uses the Palomar 48 inch Schmidt telescope. A custom-built wide-field camera provides a 47 deg ^(2) field of view and 8 s readout time, yielding more than an order of magnitude improvement in survey speed relative to its predecessor survey, the Palomar Transient Factory. We describe the design and implementation of the camera and observing system. The ZTF data system at the Infrared Processing and Analysis Center provides near-real-time reduction to identify moving and varying objects. We outline the analysis pipelines, data products, and associated archive. Finally, we present on-sky performance analysis and first scientific results from commissioning and the early survey. ZTF’s public alert stream will serve as a useful precursor for that of the Large Synoptic Survey Telescope.

Eric C. Bellm↗

Lunar Surface Electric Potential Changes Associated with Traversals through the Earth's Foreshock

We report an analysis of one year of Suprathermal Ion Detector Experiment (SIDE) Total Ion Detector (TID) resonance events observed between January 1972 and January 1973. The study includes only those events during which upstream solar wind conditions were readily available. The analysis shows that these events are associated with lunar traversals through the dawn flank of the terrestrial magnetospheric bow shock. We propose that the events result from an increase in lunar surface electric potential effected by secondary electron emission due to primary electrons in the Earth's foreshock region (although primary ions may play a role as well). This work establishes (1) the lunar surface potential changes as the Moon moves through the terrestrial bow shock, (2) the lunar surface achieves potentials in the upstream foreshock region that differ from those in the downstream magnetosheath region, (3) these differences can be explained by the presence of energetic electron beams in the upstream foreshock region and (4) if this explanation is correct, the location of the Moon with respect to the terrestrial bow shock influences lunar surface potential.

Collier, Michael R.↗