Search NASA⌕ Search

SEARCH · Search NASA

Results for “base editing”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 253 records · Page 14

ARCS: Agentic Retrieval-Augmented Code Synthesis with Iterative Refinement

Agentic Retrieval-Augmented Code Synthesis with Iterative RefinementIn supercomputing, efficient and optimized code generation is essential to leverage high-performance systems effectively. We have developed Agentic Retrieval-Augmented Code Synthesis (ARCS), an advanced framework for accurate, robust, and efficient code generation, completion, and translation. ARCS integrates Retrieval-Augmented Generation (RAG) with Chain-of-Thought (CoT) reasoning to systematically break down and iteratively refine complex programming tasks. An agent-based RAG mechanism retrieves relevant code snippets, while real-time execution feedback drives the synthesis of candidate solutions. This process is formalized as a state-action search tree optimization, balancing code correctness with editing efficiency. Evaluations on the Geeks4Geeks and HumanEval benchmarks demonstrate that ARCS significantly outperforms traditional prompting methods in translation and generation quality. By enabling scalable and precise code synthesis, ARCS offers transformative potential for automating and optimizing code development in supercomputing applications, enhancing computational resource utilization

Bhattarai, Manish [Los Alamos National Labs]↗

BinaRena: a dedicated interactive platform for human-guided exploration and binning of metagenomes

Background: Exploring metagenomic contigs and “binning” them into metagenome-assembled genomes (MAGs) are essential for the delineation of functional and evolutionary guilds within microbial communities. Despite the advances in automated binning algorithms, their capabilities in recovering MAGs with accuracy and biological relevance are so far limited. Researchers often find that human involvement is necessary to achieve representative binning results. This manual process however is expertise demanding and labor intensive, and it deserves to be supported by software infrastructure. Results: We present BinaRena, a comprehensive and versatile graphic interface dedicated to aiding human operators to explore metagenome assemblies via customizable visualization and to associate contigs with bins. Contigs are rendered as an interactive scatter plot based on various data types, including sequence metrics, coverage profiles, taxonomic assignments, and functional annotations. Various contig-level operations are permitted, such as selection, masking, highlighting, focusing, and searching. Binning plans can be conveniently edited, inspected, and compared visually or using metrics including silhouette coefficient and adjusted Rand index. Completeness and contamination of user-selected contigs can be calculated in real time. In demonstration of BinaRena’s usability, we show that it facilitated biological pattern discovery, hypothesis generation, and bin refinement in a complex tropical peatland metagenome. It enabled isolation of pathogenic genomes within closely related populations from the gut microbiota of diarrheal human subjects. It significantly improved overall binning quality after curating results of automated binners using a simulated marine dataset. Conclusions: BinaRena is an installation-free, dependency-free, client-end web application that operates directly in any modern web browser, facilitating ease of deployment and accessibility for researchers of all skill levels. The program is hosted at https://github.com/qiyunlab/binarena, together with documentation, tutorials, example data, and a live demo. It effectively supports human researchers in intuitive interpretation and fine tuning of metagenomic data.

59 BASIC BIOLOGICAL SCIENCES↗

Mission safety evaluation report for STS-37, postflight edition

STS-37/Atlantis was launched on April 5, 1991 from Kennedy Space Center launch complex 39B at 9:23 a.m. Eastern Standard Time (EST). Launch was delayed 4 minutes 45 seconds because of safety concerns about the low cloud ceiling and the wind direction in the potential blast area. Based on the limited number and type of inflight anomalies encountered, the Space Shuttle operated satisfactorily throughout the STS-37 mission. A contingency EVA was performed by the crew on Flight Day (FD) 3 to free a sticky Gamma Ray Observatory (GRO) high gain antenna, after which the GRO primary payload was successfully deployed by the Orbiter's Remote Manipulator System. The GRO, which weighed just over 35,000 lbs, was the heaviest NASA science satellite ever deployed by the Space Shuttle into low Earth orbit. The scheduled entry/landing on FD 6 was waved off for one day due to high wind conditions at Edwards Air Force Base. Atlantis landed on FD 7, 11 April 1991 on Edwards AFB lakebed runway 33 at 9:55 a.m. Eastern Daylight Time.

Hill, William C.↗

Classifying planetary cloudiness with an updated set of MODIS Cloud Regimes

We present an updated Cloud Regime (CR) dataset based on Moderate resolution Imaging Spectroradiometer (MODIS) Collection 6.1 cloud products, specifically joint histograms that partition cloud fraction within distinct combinations of cloud top pressure and cloud optical thickness ranges. The paper focuses on an edition of the CR dataset derived from our own aggregation of MODIS pixel-level cloud retrievals on an equal area grid and pre-specified 3-hour UTC intervals that spatiotemporally match International Satellite Cloud Climatology Project (ISCCP) gridded cloud data. The other edition comes from the 1-degree daily aggregation provided by standard MODIS Level-3 data, as in previous versions of the MODIS CRs, for easier use with datasets mapped on equal angle grids. Both editions consist of 11 clusters whose centroids are nearly identical.We provide a physical interpretation of the new CRs and aspects of their climatology that have not been previously examined, such as seasonal and interannual variability of CR frequency of occurrence. We also examine the makeup and precipitation properties of the CRs assisted by independent datasets originating from active observations, and provide a first glimpse of how MODIS CRs relate to clouds as seen by ISCCP.

MODIS↗

NASA Tech Briefs, October 2013

Topics include: A Short-Range Distance Sensor with Exceptional Linearity; Miniature Trace Gas Detector Based on Microfabricated Optical Resonators; Commercial Non-Dispersive Infrared Spectroscopy Sensors for Sub-Ambient Carbon Dioxide Detection; Fast, Large-Area, Wide-Bandgap UV Photodetector for Cherenkov Light Detection; Mission Data System Java Edition Version 7; Adaptive Distributed Environment for Procedure Training (ADEPT); LEGEND, a LEO-to-GEO Environment Debris Model; Electronics/Computers; Millimeter-Wave Localizers for Aircraft-to-Aircraft Approach Navigation; Impedance Discontinuity Reduction Between High-Speed Differential Connectors and PCB Interfaces; SpaceCube Version 1.5; High-Pressure Lightweight Thrusters; Non-Magnetic, Tough, Corrosion- and Wear-Resistant Knives From Bulk Metallic Glasses and Composites; Ambient Dried Aerogels; Applications for Gradient Metal Alloys Fabricated Using Additive Manufacturing; Passivation of Flexible YBCO Superconducting Current Lead With Amorphous SiO2 Layer; Propellant-Flow-Actuated Rocket Engine Igniter; Lightweight Liquid Helium Dewar for High-Altitude Balloon Payloads; Method to Increase Performance of Foil Bearings Through Passive Thermal Management; Unibody Composite Pressurized Structure; JWST Integrated Science Instrument Module Alignment Optimization Tool; Radar Range Sidelobe Reduction Using Adaptive Pulse Compression Technique; Digitally Calibrated TR Modules Enabling Real-Time Beamforming SweepSAR Architectures; Electro-Optic Time-to-Space Converter for Optical Detector Jitter Mitigation; Partially Transparent Petaled Mask/Occulter for Visible-Range Spectrum; Educational NASA Computational and Scientific Studies (enCOMPASS); Coarse-Grain Bandwidth Estimation Scheme for Large-Scale Network; Detection of Moving Targets Using Soliton Resonance Effect; High-Efficiency Nested Hall Thrusters for Robotic Solar System Exploration; High-Voltage Clock Driver for Photon-Counting CCD Characterization; Development of the Code RITRACKS; and Enabling Microliquid Chromatography by Microbead Packing of Microchannels.

Source record↗

A Novel 'Smart Microchip Proppants' Technology for Precision Diagnostics of Hydraulic Fracture Networks (Edited Final Report)

This project introduces innovative technology to improve subsurface characterization, visualization, and diagnostics of unconventional reservoirs (fossil resources). Through a collaborative effort involving the University of Kansas, UCLA, MicroSilicon Inc., and EOG Resources, the project aims to deliver precision diagnostics for hydraulic fractures using novel high-resolution imaging technology based on smart microchip proppants. Additionally, it seeks to enhance the accuracy and predictability of integrated numerical, and machine-learning modeling techniques for hydraulic fracture characterization and simulation. This groundbreaking technology addresses significant gaps in understanding unconventional and tight reservoir behavior and optimizing well-completion strategies, enabling more cost-efficient recovery of unconventional resources.

02 PETROLEUM↗

Improved GEO Derived LW Broadband Fluxes for the Edition5 CERES SYN1Deg Data Product

The NASA Clouds and the Earth's Radiant Energy System (CERES) project provides over 24 years of accurately observed top-of-the-atmosphere (TOA) and surface flux data record for climate monitoring and diagnostic studies. The major level-3 data products include SSF1deg, SYN1deg, FluxByCldTyp, and EBAF data. The CERES SYN1deg product provides 1° gridded observed TOA fluxes and computed surface fluxes based on hourly GEO clouds and derived broadband TOA fluxes. The hourly GEO derived fluxes fill in the temporal gaps between the Terra and Aqua CERES observations. In order to maintain a climate quality dataset the GEO cloud and flux retrievals are consistent over the record. The CERES project is preparing for Edition 5 product providing an opportunity to improve the GEO flux retrieval algorithms. To achieve this, the GEO satellite channel radiances are calibrated against the Aqua-MODIS calibration reference. For SW the GEO channel radiances are then converted to broadband radiances using empirical and theoretical models. The SW broadband radiances are then converted to fluxes using the same CERES angular directional models. For LW the GEO channel radiances are directly converted to LW fluxes utilizing empirical models. The resulting GEO derived broadband fluxes are then normalized to the CERES fluxes regionally by regressing the instantaneous coincident flux pairs. The normalized GEO fluxes are then tied to the CERES instrument calibration, which is very stable over time. This study will attempt to improve the GEO derived LW fluxes based on machine learning technique for the future Edition5 SYN1deg data product.

Moguo Sun↗

Large Eddy Simulation and Analysis of Truck Generated Droplet Spray Transport at Bridges

Weathering steel is a particular type of steel that develops a higher level of atmospheric corrosion protection than non-weathering grades of steel. While uncoated weathering steels have the appearance of being “rusted”, there are many forms of ferric oxyhydroxides (aka. rust) that can form on steel. The predominant ferric oxyhydroxide that gives uncoated weathering steel its tightly adherent patina is goethite. The atmospheric corrosion protection comes from alloying, primarily with a copper composition greater than 0.20 percent and also small amounts of chrome, nickel, and silicon. Bare atmospheric corrosion resistant steels have been recognized as far back as 1905 with very early editions of the American Society for Testing and Materials (ASTM) A 7 “Standard Specification for Steel for Bridges and Buildings” [1]. Uncoated weathering steel (UWS) is the lowest cost corrosion protection system possible for a steel bridge based on the first cost. Provided the UWS is used in an optimal environment that allows the protective patina to form, UWS also provides the lowest life cycle cost. The protective patina on UWS does not form or degrades in continuously damp conditions, forming a higher proportion of lepidocrocite, or in the presence of a concentration of chlorine ions that is too high, forming a higher proportion of akageneite.

36 MATERIALS SCIENCE↗

Implementation of the new electron temperature model in IRI

Since the first edition of IRI in 1978, considerable efforts have been directed towards an improved and refined electron temperature description. Finally, a new empirical model was presented at the COSPAR meeting at Graz, Austria, in 1984 (Bilitza et al.). Based mainly on satellite data it highlights a more detailed diurnal, seasonal, and altitudinal variation. The implementation of this model into the IRI framework is described. A first comparison with incoherent scatter measurements indicates the improved diurnal and seasonal performance of the model.

Bilitza, D.↗

Where have all the sources gone?

In the continuing quest for an understanding of the cosmic x-ray background, the most difficult technical barrier remains the mismatch between the wavelength band in which the discrete source populations are best characterized (0.1 to 3.5 keV), and the band in which the spectrum of the background is well determined and the bulk of the energy density resides (3.5 to 50 keV). A progress report in an attempt to resolve this conundrum using the Einstein Imaging Proportional Counter (IPC) database and analysis system is presented. The IPC data base is described and how it can be used to search both for missing clusters of galaxies and highly obscured active galactic nuclei is addressed. All 4083 imaging proportional counter observations were examined after flat fielding and editing to optimize sensitivity for diffuse emission on various size scales ranging up to 10 feet. A '2 sigma source' catalog which, when filtered by matching with source catalogs from other wavelength regimes, is shown to reveal new source classes which may be significant contributors to the cosmic x-ray background is being constructed. Optimization techniques are described and the source catalogs which result are presented. Limits on the contributions both classes of sources make to resolving the discrepancy with the higher energy observations are set.

Helfand, D. J.↗

Altitude acclimatization. Citations from the International Aerospace Abstracts data base

This bibliography of citations to the international literature covers aspects of altitude acclimatization. Included are articles concerning high altitude environments, hypoxia, heart function and hemodynamic responses, physical exercise, human tolerances and reactions, physiological responses, and oxygen consumption. This updated bibliography contains 164 citations, 35 of which are new entries to the previous edition.

Mauk, S. C.↗

Standard Operating Procedure for Optimal Deployment of Meteorological Instrumentation Within the Solar Radiation Research Laboratory: 2024 Edition

The objective of the National Renewable Energy Laboratory's (NREL's) Solar Radiation Research Laboratory (SRRL) is to collect and use high-quality solar radiation data sets for research leading to the widespread adoption of solar technologies. To appropriately populate and track the diverse array of instruments at the NREL-SRRL, NREL has established a Standard Operating Procedure (SOP) for optimal instrument deployment within the SRRL for both the Baseline Measurement System (BMS) and the Research Measurement System (RMS). Using best practices methodologies, the NREL-SRRL maintains a varied and extensive array of solar monitoring equipment to test, evaluate, and characterize the solar sensors used by federal and international agencies as well as the solar industry to determine the solar resource. The SOP provides the industry with guidance for solar resource assessment and is used for procedures in the long-term continuous monitoring of legacy instruments alongside state-of-the-art instruments. Based on the SOP, instruments are annually evaluated for continued deployment. Instruments that do not meet the SOP criteria are decommissioned, and new instruments that meet the criteria are deployed. Streamlining and optimizing the use of this facility ensures that the lab continues to be a world-leading solar calibration and measurement facility. This 2024 edition includes updates to the appendices to reflect the instrument changes from one year to another.

14 SOLAR ENERGY↗

Standard Operating Procedure for Optimal Deployment of Meteorological Instrumentation Within the Solar Radiation Research Laboratory: 2025 Edition

The objective of the National Renewable Energy Laboratory's (NREL's) Solar Radiation Research Laboratory (SRRL) is to collect and use high-quality solar radiation data sets for research leading to the widespread adoption of solar technologies. To appropriately populate and track the diverse array of instruments at the NREL-SRRL, NREL has established a Standard Operating Procedure (SOP) for optimal instrument deployment within the SRRL for both the Baseline Measurement System (BMS) and the Research Measurement System (RMS). Using best practices methodologies, the NREL-SRRL maintains a varied and extensive array of solar monitoring equipment to test, evaluate, and characterize the solar sensors used by federal and international agencies as well as the solar industry to determine the solar resource. The SOP provides the industry with guidance for solar resource assessment and is used for procedures in the long-term continuous monitoring of legacy instruments alongside state-of-the-art instruments. Based on the SOP, instruments are annually evaluated for continued deployment. Instruments that do not meet the SOP criteria are decommissioned, and new instruments that meet the criteria are deployed. Streamlining and optimizing the use of this facility ensures that the lab continues to be a world-leading solar calibration and measurement facility. This 2025 edition includes updates to the appendices to describe the current instrumentation of the NREL-SRRL.

14 SOLAR ENERGY↗

Deep Learning‐Assisted Automated Single Cell Electroporation Platform for Effective Genetic Manipulation of Hard‐to‐Transfect Cells

Abstract Genome engineering of cells using CRISPR/Cas systems has opened new avenues for pharmacological screening and investigating the molecular mechanisms of disease. A critical step in many such studies is the intracellular delivery of the gene editing machinery and the subsequent manipulation of cells. However, these workflows often involve processes such as bulk electroporation for intracellular delivery and fluorescence activated cell sorting for cell isolation that can be harsh to sensitive cell types such as human‐induced pluripotent stem cells (hiPSCs). This often leads to poor viability and low overall efficacy, requiring the use of large starting samples. In this work, a fully automated version of the nanofountain probe electroporation (NFP‐E) system, a nanopipette‐based single‐cell electroporation method is presented that provides superior cell viability and efficiency compared to traditional methods. The automated system utilizes a deep convolutional network to identify cell locations and a cell‐nanopipette contact algorithm to position the nanopipette over each cell for the application of electroporation pulses. The automated NFP‐E is combined with microconfinement arrays for cell isolation to demonstrate a workflow that can be used for CRISPR/Cas9 gene editing and cell tracking with potential applications in screening studies and isogenic cell line generation.

59 BASIC BIOLOGICAL SCIENCES↗

The staging system: Display and edit module

The Display and Edit (D and E) Module described is one of six major modules being developed for the STAGING (STructural Analysis through Generalized INteractive Graphics) System. Several remarks are included concerning the computer environment and the architecture of the data base. The utility of this module is emphasized.

Edwards, E.↗

Recent experiences with implementing a video based six degree of freedom measurement system for airplane models in a 20 foot diameter vertical spin tunnel

A model space positioning system (MSPS), a state-of-the-art, real-time tracking system to provide the test engineer with on line model pitch and spin rate information, is described. It is noted that the six-degree-of-freedom post processor program will require additional programming effort both in the automated tracking mode for high spin rates and in accuracy to meet the measurement objectives. An independent multicamera system intended to augment the MSPS is studied using laboratory calibration methods based on photogrammetry to characterize the losses in various recording options. Data acquired to Super VHS tape encoded with Vertical Interval Time Code and transcribed to video disk are considered to be a reasonable priced choice for post editing and processing video data.

Snow, Walter L.↗

MicroShell Minimalist Shell for Xilinx Microprocessors

MicroShell is a lightweight shell environment for engineers and software developers working with embedded microprocessors in Xilinx FPGAs. (MicroShell has also been successfully ported to run on ARM Cortex-M1 microprocessors in Actel ProASIC3 FPGAs, but without project-integration support.) Micro Shell decreases the time spent performing initial tests of field-programmable gate array (FPGA) designs, simplifies running customizable one-time-only experiments, and provides a familiar-feeling command-line interface. The program comes with a collection of useful functions and enables the designer to add an unlimited number of custom commands, which are callable from the command-line. The commands are parameterizable (using the C-based command-line parameter idiom), so the designer can use one function to exercise hardware with different values. Also, since many hardware peripherals instantiated in FPGAs have reasonably simple register-mapped I/O interfaces, the engineer can edit and view hardware parameter settings at any time without stopping the processor. MicroShell comes with a set of support scripts that interface seamlessly with Xilinx's EDK tool. Adding an instance of MicroShell to a project is as simple as marking a check box in a library configuration dialog box and specifying a software project directory. The support scripts then examine the hardware design, build design-specific functions, conditionally include processor-specific functions, and complete the compilation process. For code-size constrained designs, most of the stock functionality can be excluded from the compiled library. When all of the configurable options are removed from the binary, MicroShell has an unoptimized memory footprint of about 4.8 kB and a size-optimized footprint of about 2.3 kB. Since MicroShell allows unfettered access to all processor-accessible memory locations, it is possible to perform live patching on a running system. This can be useful, for instance, if a bug is discovered in a routine but the system cannot be rebooted: Shell allows a skilled operator to directly edit the binary executable in memory. With some forethought, MicroShell code can be located in a different memory location from custom code, permitting the custom functionality to be overwritten at any time without stopping the controlling shell.

Werne, Thomas A.↗

Designing Scenarios for Controller-in-the-Loop Air Traffic Simulations

Well prepared traffic scenarios contribute greatly to the success of controller-in-the-loop simulations. This paper describes each stage in the design process of realistic scenarios based on real-world traffic, to be used in the Airspace Operations Laboratory for simulations within the Air Traffic Management Technology Demonstration 1 effort. The steps from the initial analysis of real-world traffic, to the editing of individual aircraft records in the scenario file, until the final testing of the scenarios before the simulation conduct, are all described. The iterative nature of the design process and the various efforts necessary to reach the required fidelity, as well as the applied design strategies, challenges, and tools used during this process are also discussed.

ATD1↗