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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 253 records · Page 14
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NASA NTRS1985

Summary electrophoretic data base on human embryonic kidney cell strain 8514

To properly plan the electrophoresis equipment verification test (EEVT) and continuous flow electrophoresis system (CFES) experiments with human embryonic kidney cells, first a candidate cell lot had to be chosen on the basis of electrophoretic heterogeneity, growth potential, cytogenetics, and urokinase production. Cell lot 8514 from MA Bioproducts, Inc. was chosen for this purpose, and several essential analytical electrophoresis experiments were performed to test its final suitability for these experiments.

Plank, L. D.↗
NASA NTRS1985

Free zone electrophoresis simulation of static column electrophoresis in microgravity on shuttle flight STS-3

Experiments were designed to replicate, as closely as possible in 1-G, the conditions of the STS-3 red blood cell (RBC) experiments. Free zone electrophoresis was the method of choice, since it minimizes the role of gravity in cell migration. The physical conditions of the STS-3 experiments were used, and human and rabbit RBC's fixed by the same method were the test particles. The effects of cell concentration, electroosmotic mobility, and sample composition were tested in order to seek explanations for the STS-3 results and to provide data on cell concentration effects for future zero-G separation on the continuous-flow zero-G electrophoretics separator.

Todd, P. W.↗
NASA NTRS1985

Microgravity electrophoresis: A study of the factors that affect free-fluid separation

Electrophoresis experiments have been performed in the microgravity environment of the Space Shuttle. Test particles (fixed human and rabbit erythrocytes) migrated as expected in a static column and test macromolecules (human serum albumin, ovalbumin, hemoglobin A, and Pneumococcus polysaccharide 6B) migrated as expected in a continuous flow apparatus. The concentrations studied exceeded those that can be used in free-fluid separation and purification processes at unit gravity.

Source record↗
NASA NTRS1985

Properties of electrophoretic fractions of human embryonic kidney cells separated on space shuttle flight STS-8

Suspensions of cultured primary human embryonic kidney cells were subjected to continuous flow electrophoresis on Space Shuttle flight STS-8. The objectives of the experiments were to obtain electrophoretically separated fractions of the original cell populations and to test these fractions for the amount and kind of urokinase (a kidney plasminogen activator that is used medically for digesting blood clots), the morphologies of cells in the individual fractions, and their cellular electrophoretic mobilities after separation and subsequent proliferation. Individual fractions were successfully cultured after return from orbit, and they were found to differ substantially from one another and from the starting sample with respect to all of these properties.

Morrison, D. R.↗
NASA NTRS1985

Stall recovery control strategy methodology and results

The nonrecoverable stall condition in high performance turbofan engines is characterized by thrust loss, rising turbine temperatures, high pressure compressor rotating stall, and loss of engine control. Stall recovery control is presently investigated by means of an engine system computer model capable of either surge or rotating stall postinstability operation. Several techniques are examined which can yield rapid recovery from stall; a composite strategy which involves reduction of engine speed to idle while simultaneously opening the 10th- and 14th-stage compressor bleed ports allowed recovery to speeds slightly higher than idle while combustor fuel flow continued.

Hopf, W. R.↗
NASA NTRS1985

Cloud Microphysics by Thermal Wave Methods

This experiments series is the first application of a low-gravity experimental technique to the study of cloud microphysics. The low-gravity environment is provided by the parabolic maneuver of NASA's KC-135 aircraft. The primary objective is to compare experimental observations of cloud droplet growth and evaporation in a convection free environment with a numerical model of the process. Beyond that, the work also involves the development and testing of low-gravity research techniques. In particular, passive methods of thermal control have been devised and used effectively. The study to date has shown that the method is particularly suitable for looking at interactions between adjoining portions of the cloud drop field and interactions of the drop field with a solid interface. After final analysis of the data, it is expected the results will shed light on the development of cloud droplet size spectra in natural clouds as well as the performance of certain types of cloud physics instrumentation, particularly continuous flow diffusion chambers and loud condensation nuclei counters.

Anderson, B. J.↗
NASA NTRS1985

Biochemical Assays of Cultured Cells

Subpopulations of human embryonic kidney cells isolated from continuous flow electrophoresis experiments performed at McDonnell Douglas and on STS-8 have been analyzed. These analyses have included plasminogen activator assays involving indirect methodology on fibrin plated and direct methodology using chromogenic substrates. Immunological studies were performed and the conditioned media for erythropoietin activity and human granulocyte colony stimulating (HGCSF) activity was analyzed.

Barlow, G. H.↗
NASA NTRS1986

Spontaneous ignition delay characteristics of hydrocarbon fuel-air mixtures

The influence of pressure on the autoignition characteristics of homogeneous mixtures of hydrocarbon fuels in air is examined. Autoignition delay times are measured for propane, ethylene, methane, and acetylene in a continuous flow apparatus featuring a multi-point fuel injector. Results are presented for mixture temperatures from 670K to 1020K, pressures from 1 to 10 atmospheres, equivalence ratios from 0.2 to 0.7, and velocities from 5 to 30 m/s. Delay time is related to pressure, temperature, and fuel concentration by global reaction theory. The results show variations in global activation energy from 25 to 38 kcal/kg-mol, pressure exponents from 0.66 to 1.21, and fuel concentration exponents from 0.19 to 0.75 for the fuels studied. These results are generally in good agreement with previous studies carried out under similar conditions.

Lefebvre, A. H.↗
NASA NTRS1985

Oxygen extraction from lunar materials - An experimental test of an ilmenite reduction process

The reaction of ilmenite with hydrogen to produce water has been studied experimentally in order to evaluate the effectiveness of using a cold trap to improve yields in a continuous flow process. Yields were enhanced, but not to the degree observed in batch processing systems. The terrestrial simulant used in these studies contained traces of iron sulfide, which released H2S during processing with a deleterious effect on several components of the test system. More sophisticated testing should be undertaken to obtain kinetic data and attention given to the removal of sulfides in the pre-process beneficiation.

Williams, R. J.↗
NASA NTRS1987

Preparative electrophoresis for space

A premise of continuous flow electrophoresis is that removal of buoyancy-induced thermal convection caused by axial and lateral temperature gradients results in ideal performance of these instruments in space. Although these gravity dependent phenomena disturb the rectilinear flow in the separation chamber when high voltage gradients or thick chambers are used, distortion of the injected sample stream due to electrohydrodynamic effects cause major broadening of the separated bands. The electrophoresis separation process is simple, however flow local to the sample filament produced by the applied electric field have not been considered. These electrohydrodynamic flows distort the sample stream and limit the separation. Also, electroosmosis and viscous flow combine to further distort the process. A moving wall concept is being proposed for space which will eliminate and control the disturbances. The moving wall entrains the fluid to move as a rigid body and produces a constant residence time for all samples distributed across the chamber thickness. The moving wall electrophoresis chamber can only be operated in space because there is no viscous flow in the chamber to stabilize against thermal convection.

Rhodes, Percy H.↗
NASA NTRS1987

Oxygen chemisorption cryogenic refrigerator

The present invention relates to a chemisorption compressor cryogenic refrigerator which employs oxygen to provide cooling at 60 to 100 K. The invention includes dual vessels containing an oxygen absorbent material, alternately heated and cooled to provide a continuous flow of high pressure oxygen, multiple heat exchangers for precooling the oxygen, a Joule-Thomson expansion valve system for expanding the oxygen to partially liquefy it and a liquid oxygen pressure vessel. The primary novelty is that, while it was believed that once oxygen combined with an element or compound the reaction could not reverse to release gaseous oxygen, in this case oxygen will indeed react in a reversible fashion with certain materials and will do so at temperatures and pressures which make it practical for incorporation into a cryogenic refrigeration system.

Jones, Jack A.↗
NASA NTRS1988

Electrochemical processing of solid waste

The investigation into electrolysis as a means of waste treatment and recycling on manned space missions is described. The electrochemical reactions of an artificial fecal waste mixture was examined. Waste electrolysis experiments were performed in a single compartment reactor, on platinum electrodes, to determine conditions likely to maximize the efficiency of oxidation of fecal waste material to CO2. The maximum current efficiencies for artificial fecal waste electrolysis to CO2 was found to be around 50 percent in the test apparatus. Experiments involving fecal waste oxidation on platinum indicates that electrodes with a higher overvoltage for oxygen evolution such as lead dioxide will give a larger effective potential range for organic oxidation reactions. An electrochemical packed column reactor was constructed with lead dioxide as electrode material. Preliminary experiments were performed using a packed-bed reactor and continuous flow techniques showing this system may be effective in complete oxidation of fecal material. The addition of redox mediator Ce(3+)/Ce(4+) enhances the oxidation process of biomass components. Scientific literature relevant to biomass and fecal waste electrolysis were reviewed.

Bockris, J. OM.↗
NASA NTRS1984

Separation of cells from the rat anterior pituitary gland

Data concerned with analyzing the cellular organization of the rat anterior pituitary gland are examined. The preparation of the cell suspensions and the methods used to separate pituitary cell types are described. Particular emphasis is given to velocity sedimentation at unit gravity, density gradient centrifugation, affinity methods, fluorescence activated cell sorting, and density gradient and continuous-flow electrophoresis. The difficulties encountered when attempting to compare data from different pituitary cell separation studies are discussed, and results from various experiments are presented. The functional capabilities of the separated cell populations can be tested in various culture systems.

Hymer, W. C.↗
NASA NTRS1988

Comparison of bioseparation methods for microgravity experiments

The efficiency of the 1-g version of the continuous-flow electrophoresis (CFE) system flown on Space Shuttle missions is compared with the efficiency of a commercial CFE for separating living cells (human kidney, liver, and pituitary-gland cells and T-lymphocytes). In addition, the CFE system and a reciprocal isoelectric focusing (RIEF) system are compared with respect to protein pyrification efficiency. Correlations were made among electrophoretic mobilities (EPMs), secretory functions of cells, and input sample concentrations. A significant reduction in mean and range EPM was observed when input sample concentrations exceeded a low threshohold. This effect was not observed in microgravity experiments conducted at sample concentrations three times greater than the threshold for the controls. Comparison of CFE and RIEF methods showed that there are apparent advantages for each method depending on the product. For example, RIEF purification of urokinase removed more protein impurities, but focused the enzyme at a pH different than the enzyme's known isoelectric point.

Morrison, Dennis R.↗
NASA NTRS1988

Evaluation of a flexible wall testing technique to minimize wall interferences in the NASA Langley 0.3-m Transonic Cryogenic Tunnel

Free air simulations in conventional transonic wind tunnels require improvement which adaptive wall testing techniques can provide primarily by minimizing wall interferences. In addition, these techniques offer other substantial advantages such as increased Reynolds number capability and a reduction in tunnel drive power. The combination of an adaptive wall test section with a continuous flow cryogenic wind tunnel is unique. The test section has four solid walls with two flexible walls mounted between rigid sidewalls. This modification of an existing major facility stresses the practicalities of the testing technique. These practicalities were evaluated in terms of flexible wall test section design and operation. Increased hardware and operating complexity of the new test section is offset by a significant improvement in real-time data accuracy in two-dimensional testing. Validation testing has expanded the experience with flexible walled test sections into the realms of flight Reynolds numbers and high lift. Data accuracy has been assessed with regard to test section geometry and operating tolerances. The successful evaluation of the testing technique in two-dimensional testing has proved that a production type operation is possible, with suitable control system and test section design. The 0.3-m Transonic Cryogenic Tunnel with an adaptive wall test section currently represents the most advanced two-dimensional facility anywhere.

Wolf, Stephen W. D.↗
NASA NTRS1987

Light hydrocarbons from plasma discharge in H2-He-CH4 - First results and Uranian auroral chemistry

The production of light hydrocarbons by precipitating magnetospheric electrons in the Uranian stratosphere is simulated in laboratory experiments. The products of continuous-flow glow discharges of H2-He-CH4 mixtures containing 0.0012 or 0.022 mol pct CH4 at pressures 0.63-57 mbar are trapped at 77 K, measured manometrically, and identified by gas chromatography and mass spectroscopy; the results are presented in tables and characterized in detail. The globally averaged rate for the production of higher hydrocarbons by this mechanism on Uranus is estimated as 3 x 10 to the 6th C/sq cm sec and shown to be similar to that for photochemical production, while the local rate for the auroral zones is significantly higher than the corresponding photochemical rate. A decrease in yield with increasing molecular complexity is also noted.

Thompson, W. Reid↗
NASA NTRS1983

A study of cell electrophoresis as a means of purifying growth hormone secreting cells

Growth hormone secreting cells of the rat anterior pituitary are heavily laden with granules of growth hormone and can be partialy purified on the basis of their resulting high density. Two methods of preparative cell electrophoresis were investigated as methods of enhancing the purification of growth hormone producing cells: density gradient electrophoresis and continuous flow electrophoresis. Both methods provided a two- to four-fold enrichment in growth hormone production per cell relative to that achieved by previous methods. Measurements of electrophoretic mobilities by two analytical methods, microscopic electrophoresis and laser-tracking electrophoresis, revealed very little distinction between unpurified anterior pituitary cell suspensions and somatotroph-enriched cell suspensions. Predictions calculated on the basis of analytical electrophoretic data are consistent with the hypothesis that sedimentation plays a significant role in both types of preparative electrophoresis and the electrophoretic mobility of the growth hormone secreting subpopulation of cells remains unknown.

Plank, Lindsay D.↗
NASA NTRS1988

Highlights of experience with a flexible walled test section in the NASA Langley 0.3-meter transonic cryogenic tunnel

The unique combination of adaptive wall technology with a continuous flow cryogenic wind tunnel is described. This powerful combination allows wind tunnel users to carry out two-dimensional (2-D) tests at flight Reynolds numbers with wall interferences essentially eliminated. Validation testing was conducted to support this claim using well tested symmetrical and cambered airfoils at transonic speeds and high Reynolds numbers. The test section hardware has four solid walls, with the floor and ceiling flexible. The method of adapting/shaping the floor and ceiling to eliminate top and bottom wall interference at its source is outlined. Data comparisons for different size models tested and others in several sophisticated 2-D wind tunnels are made. In addition, the effects of Reynolds number, testing at high lift with associated large flexible wall movements, the uniqueness of the adapted wall shapes, and the effects of sidewall boundary layer control are examined. The 0.3-m TCT is now the most advanced 2-D research facility anywhere.

Wolf, Stephen W. D.↗