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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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All sourcesNASA NTRSDOE OSTI
NASA NTRS2012

Stability Analysis for HIFiRE Experiments

The HIFiRE-1 flight experiment provided a valuable database pertaining to boundary layer transition over a 7-degree half-angle, circular cone model from supersonic to hypersonic Mach numbers, and a range of Reynolds numbers and angles of attack. This paper reports selected findings from the ongoing computational analysis of the measured in-flight transition behavior. Transition during the ascent phase at nearly zero degree angle of attack is dominated by second mode instabilities except in the vicinity of the cone meridian where a roughness element was placed midway along the length of the cone. The growth of first mode instabilities is found to be weak at all trajectory points analyzed from the ascent phase. For times less than approximately 18.5 seconds into the flight, the peak amplification ratio for second mode disturbances is sufficiently small because of the lower Mach numbers at earlier times, so that the transition behavior inferred from the measurements is attributed to an unknown physical mechanism, potentially related to step discontinuities in surface height near the locations of a change in the surface material. Based on the time histories of temperature and/or heat flux at transducer locations within the aft portion of the cone, the onset of transition correlated with a linear N-factor, based on parabolized stability equations, of approximately 13.5. Due to the large angles of attack during the re-entry phase, crossflow instability may play a significant role in transition. Computations also indicate the presence of pronounced crossflow separation over a significant portion of the trajectory segment that is relevant to transition analysis. The transition behavior during this re-entry segment of HIFiRE-1 flight shares some common features with the predicted transition front along the elliptic cone shaped HIFiRE-5 flight article, which was designed to provide hypersonic transition data for a fully 3D geometric configuration. To compare and contrast the crossflow dominated transition over the HIFiRE-1 and HIFiRE-5 configurations, this paper also analyzes boundary layer instabilities over a subscale model of the HIFiRE-5 flight configuration that was tested in the Mach 6 quiet tunnel facility at Purdue University.

Li, Fei↗
NASA NTRS2016

Comparison of Mesospheric Winds From a High-Altitude Meteorological Analysis System and Meteor Radar Observations During the Boreal Winters of 2009-2010 and 2012-2013

We present a study of horizontal winds in the mesosphere and lower thermosphere (MLT) during the boreal winters of 2009-2010 and 2012-2013 produced with a new high-altitude numerical weather prediction (NWP) system. This system is based on a modified version of the Navy Global Environmental Model (NAVGEM) with an extended vertical domain up to approximately 116 km altitude coupled with a hybrid four-dimensional variational (4DVAR) data assimilation system that assimilates both standard operational meteorological observations in the troposphere and satellite-based observations of temperature, ozone and water vapor in the stratosphere and mesosphere. NAVGEM-based MLT analyzed winds are validated using independent meteor radar wind observations from nine different sites ranging from 69 deg N-67 deg S latitude. Time-averaged NAVGEM zonal and meridional wind profiles between 75 and 95 km altitude show good qualitative and quantitative agreement with corresponding meteor radar wind profiles. Wavelet analysis finds that the 3-hourly NAVGEM and 1-hourly radar winds both exhibit semi-diurnal, diurnal, and quasi-diurnal variations whose vertical profiles of amplitude and phase are also in good agreement. Wavelet analysis also reveals common time-frequency behavior in both NAVGEM and radar winds throughout the Northern extra tropics around the times of major stratospheric sudden warmings (SSWs) in January 2010 and January 2013, with a reduction in semi-diurnal amplitudes beginning around the time of a mesospheric wind reversal at 60 deg N that precedes the SSW, followed by an amplification of semi-diurnal amplitudes that peaks 10-14 days following the onset of the mesospheric wind reversal. The initial results presented in this study demonstrate that the wind analyses produced by the high altitude NAVGEM system accurately capture key features in the observed MLT winds during these two boreal winter periods.

McCormack, J.↗
NASA NTRS2004

Buoyancy Effects on Flow Structure and Instability of Low-Density Gas Jets

A low-density gas jet injected into a high-density ambient gas is known to exhibit self-excited global oscillations accompanied by large vortical structures interacting with the flow field. The primary objective of the proposed research is to study buoyancy effects on the origin and nature of the flow instability and structure in the near-field of low-density gas jets. Quantitative rainbow schlieren deflectometry, Computational fluid dynamics (CFD) and Linear stability analysis were the techniques employed to scale the buoyancy effects. The formation and evolution of vortices and scalar structure of the flow field are investigated in buoyant helium jets discharged from a vertical tube into quiescent air. Oscillations at identical frequency were observed throughout the flow field. The evolving flow structure is described by helium mole percentage contours during an oscillation cycle. Instantaneous, mean, and RMS concentration profiles are presented to describe interactions of the vortex with the jet flow. Oscillations in a narrow wake region near the jet exit are shown to spread through the jet core near the downstream location of the vortex formation. The effects of jet Richardson number on characteristics of vortex and flow field are investigated and discussed. The laminar, axisymmetric, unsteady jet flow of helium injected into air was simulated using CFD. Global oscillations were observed in the flow field. The computed oscillation frequency agreed qualitatively with the experimentally measured frequency. Contours of helium concentration, vorticity and velocity provided information about the evolution and propagation of vortices in the oscillating flow field. Buoyancy effects on the instability mode were evaluated by rainbow schlieren flow visualization and concentration measurements in the near-field of self-excited helium jets undergoing gravitational change in the microgravity environment of 2.2s drop tower at NASA John H. Glenn Research Center. The jet Reynolds number was varied from 200 to 1500 and jet Richardson number was varied from 0.72 to 0.002. Power spectra plots generated from Fast Fourier Transform (FFT) analysis of angular deflection data acquired at a temporal resolution of 1000Hz reveal substantial damping of the oscillation amplitude in microgravity at low Richardson numbers (~0.002). Quantitative concentration data in the form of spatial and temporal evolutions of the instability data in Earth gravity and microgravity reveal significant variations in the jet flow structure upon removal of buoyancy forces. Radial variation of the frequency spectra and time traces of helium concentration revealed the importance of gravitational effects in the jet shear layer region. Linear temporal and spatio-temporal stability analyses of a low-density round gas jet injected into a high-density ambient gas were performed by assuming hyper-tan mean velocity and density profiles. The flow was assumed to be non parallel. Viscous and diffusive effects were ignored. The mean flow parameters were represented as the sum of the mean value and a small normal-mode fluctuation. A second order differential equation governing the pressure disturbance amplitude was derived from the basic conservation equations. The effects of the inhomogeneous shear layer and the Froude number (signifying the effects of gravity) on the temporal and spatio-temporal results were delineated. A decrease in the density ratio (ratio of the density of the jet to the density of the ambient gas) resulted in an increase in the temporal amplification rate of the disturbances. The temporal growth rate of the disturbances increased as the Froude number was reduced. The spatio-temporal analysis performed to determine the absolute instability characteristics of the jet yield positive absolute temporal growth rates at all Fr and different axial locations. As buoyancy was removed (Fr . 8), the previously existing absolute instability disappeared at all locations establhing buoyancy as the primary instability mechanism in self-excited low-density jets.

Pasumarthi, Kasyap Sriramachandra↗
NASA NTRS2006

Thrust Stand for Electric Propulsion Performance Evaluation

An electric propulsion thrust stand capable of supporting testing of thrusters having a total mass of up to 125 kg and producing thrust levels between 100 microN to 1 N has been developed and tested. The design features a conventional hanging pendulum arm attached to a balance mechanism that converts horizontal deflections produced by the operating thruster into amplified vertical motion of a secondary arm. The level of amplification is changed through adjustment of the location of one of the pivot points linking the system. Response of the system depends on the relative magnitudes of the restoring moments applied by the displaced thruster mass and the twisting torsional pivots connecting the members of the balance mechanism. Displacement is measured using a non-contact, optical linear gap displacement transducer and balance oscillatory motion is attenuated using a passive, eddy-current damper. The thrust stand employs an automated leveling and thermal control system. Pools of liquid gallium are used to deliver power to the thruster without using solid wire connections, which can exert undesirable time-varying forces on the balance. These systems serve to eliminate sources of zero-drift that can occur as the stand thermally or mechanically shifts during the course of an experiment. An in-situ calibration rig allows for steady-state calibration before, during and after thruster operation. Thrust measurements were carried out on a cylindrical Hall thruster that produces mN-level thrust. The measurements were very repeatable, producing results that compare favorably with previously published performance data, but with considerably smaller uncertainty.

Polzin, Kurt A.↗
NASA NTRSDate not supplied

Influence of the Mid-Holocene Green Sahara on South American Climate

Paleoclimatic data from pollen, speleothem and sedimentary records indicate a weakening of the South American Monsoon in the mid-Holocene (MH: 6,000 years BP), which occurred coevally with the intensification of the West African Monsoon. The reduction in precipitation over South America is also simulated by various models which account for changes in orbital forcings and greenhouse gas concentrations between the present day and the mid-Holocene. However, most model studies do not account for vegetation changes, which precludes realistic simulations, especially due to the presence and far-afield impacts of the Green Sahara. In this study, we evaluate precipitation over South America during the mid-Holocene from four models, namely – EC-Earth 3.1, iCESM 1.2, CCSM4-Toronto and GISS Model E2.1-G. Two scenarios are considered for each model – an MHPMIP scenario simulated with the mid-Holocene orbital parameters and greenhouse gas concentrations with vegetation prescribed to pre-industrial conditions, as well as an MHGS (Green Sahara) scenario which additionally considers factors such as enhanced vegetation, reduced dust, presence of lakes, and land and soil feedbacks. Incorporating the Green Sahara leads to an amplification of the drying signal over South America and brings it more in line with the proxy reconstructions. We also present a semi-quantitative assessment of the proxy-model agreement. Our work indicates that it is important to consider changes over northern Africa while studying South American climate of the mid-Holocene.

GISS-E2-1 model↗
NASA NTRS1993

Lee waves: Benign and malignant

The flow of an incompressible fluid over an obstacle will produce an oscillation in which buoyancy is the restoring force, called a gravity wave. For disturbances of this scale, the atmosphere may be treated as dynamically incompressible, even though there exists a mean static upward density gradient. Even in the linear approximation - i.e., for small disturbances - this model explains a great many of the flow phenomena observed in the lee of mountains. However, nonlinearities do arise importantly, in three ways: (1) through amplification due to the decrease of mean density with height; (2) through the large (scaled) size of the obstacle, such as a mountain range; and (3) from dynamically singular levels in the fluid field. These effects produce a complicated array of phenomena - large departure of the streamlines from their equilibrium levels, high winds, generation of small scales, turbulence, etc. - that present hazards to aircraft and to lee surface areas. The nonlinear disturbances also interact with the larger-scale flow in such a manner as to impact global weather forecasts and the climatological momentum balance. If there is no dynamic barrier, these waves can penetrate vertically into the middle atmosphere (30-100 km), where recent observations show them to be of a length scale that must involve the coriolis force in any modeling. At these altitudes, the amplitude of the waves is very large, and the phenomena associated with these wave dynamics are being studied with a view to their potential impact on high performance aircraft, including the projected National Aerospace Plane (NASP). The presentation shows the results of analysis and of state-of-the-art numerical simulations, validated where possible by observational data, and illustrated with photographs from nature.

Wurtele, M. G.↗
NASA NTRS1992

The x ray variability of NGC6814: Power spectra

Simulation techniques are used to obtain the X-ray variability power spectrum of unevenly sampled GINGA data from NGC6814. A simple power law is not an adequate description of the power spectrum, with the residuals showing excess power on timescales consistent with the periodicity seen in EXOSAT observations of this object. However the shape of the folded lightcurve is very different, with 3 main peaks, two of which are separated by an extremely sharp dip instead of the single peak and small harmonic structure observed by EXOSAT. Using the dip as a fiducial mark, a second GINGA observation of this source taken one year later is found to be consistent with being completely periodic and phase coherent with this first GINGA observation. Thus the period is consistent with being constant over a period of 6 years, but phase coherence is only maintained on timescales of approximately 1 year. Over 75 percent of the total source variability is due to the periodic component (r.m.s. amplitude of 36 percent). The residual variability can be described as the more usual 'flicker noise' f(exp -1.1) powerlaw. This shows no apparent high frequency break on timescales greater than 300 seconds. Subtle differences in the shape of the folded light curve with energy, and the very large amount of power in the periodic component suggest occultation as its origin, though amplification of variability from an X-ray emitting 'hot spot' at the disk inner radius through gravitational lensing is also possible. The former suffers from the very arbitrary nature of the periodic timescale, while the latter is unattractive as it cannot simply explain the lack of high frequency break in the residual power. That these models probably fail to provide an adequate explanation may be due to the added complexity of anisotropy of the X-ray emission, suggested by the discrepancy between the lack of soft photons implied by the flat spectrum and the copious source of soft photons available from reprocessing in the iron line producing material.

Done, C.↗
DOE OSTI2024

The circular bioeconomy: a driver for system integration

Background: Human and earth system modeling, traditionally centered on the interplay between the energy system and the atmosphere, are facing a paradigm shift. The Intergovernmental Panel on Climate Change’s mandate for comprehensive, cross-sectoral climate action emphasizes avoiding the vulnerabilities of narrow sectoral approaches. Our study explores the circular bioeconomy, highlighting the intricate interconnections among agriculture, forestry, aquaculture, technological advancements, and ecological recycling. Collectively, these sectors play a pivotal role in supplying essential resources to meet the food, material, and energy needs of a growing global population. We pose the pertinent question of what it takes to integrate these multifaceted sectors into a new era of holistic systems thinking and planning. Results: The foundation for discussion is provided by a novel graphical representation encompassing statistical data on food, materials, energy flows, and circularity. This representation aids in constructing an inventory of technological advancements and climate actions that have the potential to significantly reshape the structure and scale of the economic metabolism in the coming decades. In this context, the three dominant mega-trends—population dynamics, economic developments, and the climate crisis—compel us to address the potential consequences of the identified actions, all of which fall under the four categories of substitution, efficiency, sufficiency, and reliability measures. Substitution and efficiency measures currently dominate systems modeling. Including novel bio-based processes and circularity aspects might require only expanded system boundaries. Conversely, paradigm shifts in systems engineering are expected to center on sufficiency and reliability actions. Effectively assessing the impact of sufficiency measures will necessitate substantial progress in inter- and transdisciplinary collaboration, primarily due to their non-technological nature. In addition, placing emphasis on modeling the reliability and resilience of transformation pathways represents a distinct and emerging frontier that highlights the significance of an integrated network of networks. Conclusions: Existing and emerging circular bioeconomy practices can serve as prime examples of system integration. These practices facilitate the interconnection of complex biomass supply chain networks with other networks encompassing feedstock-independent renewable power, hydrogen, CO 2 , water, and other biotic, abiotic, and intangible resources. Elevating the prominence of these connectors will empower policymakers to steer the amplification of synergies and mitigation of tradeoffs among systems, sectors, and goals.

09 BIOMASS FUELS↗
NASA NTRS2020

Streak Instabilities on HIFiRE-5 Elliptic Cone

The Hypersonic International Flight Research and Experimentation (HIFiRE) 5 flight experiment by the Air Force Research Laboratories and Australian Defense Science and Technology Organization yielded inflight, hypersonic transition data for a canonical, 3D configuration in the form of an elliptic cone at various combinations of angle-of-attack and yaw. Previous analysis has shown that boundary-layer stability calculations for the as-flown, zero-yaw flight conditions are able to capture the second mode dominated transition along the attachment line as well as the crossflow induced transition in between the attachment line and the minor axis symmetry plane. However, there is still a need to understand the transition in the vicinity of the minor axis plane of symmetry, where the boundary layer thickness exhibits an azimuthally compact bulge due to the collision of the secondary flow approaching from the attachment lines on both sides. This paper reports a computational analysis of the stability of the boundary layer flow along the bulge regions on both the leeward and windward sides of the vehicle surface. At a representative flight condition corresponding to a time instant of t = 514.813 seconds, computations are performed to identify several different unstable modes within the bulge regions, with the eventual goal of developing a linear stability correlation for the transition onset near the minor axis symmetry plane. The most amplified flow instabilities in the pretransitional region correspond to a “stem” mode that is concentrated within a pair of nearly vertical shear layers below the cap of the mushroom shaped bulge in the mean velocity contours. An excellent agreement is found between the predictions of instability amplification based on the plane marching parabolized stability equations (PSE) and the direct numerical solutions (DNS). Given the large number of unstable modes within the bulge region, the observed agreement demonstrates, for first time, that the PSE can provide accurate predictions for the development of flow instabilities in streak-like flow features over three-dimensional bodies. The peak N-factor of approximately 15 at the transition location estimated from the thermocouple measurements along the leeward centerline is close to the transition N-factor for Mack mode transition during the HIFiRE-1 circular cone flight experiment with nearly zero degrees angle of attack.

Meelan Choudhari↗
NASA NTRS1998

Chimeric calcium/calmodulin-dependent protein kinase in tobacco: differential regulation by calmodulin isoforms

cDNA clones of chimeric Ca2+/calmodulin-dependent protein kinase (CCaMK) from tobacco (TCCaMK-1 and TCCaMK-2) were isolated and characterized. The polypeptides encoded by TCCaMK-1 and TCCaMK-2 have 15 different amino acid substitutions, yet they both contain a total of 517 amino acids. Northern analysis revealed that CCaMK is expressed in a stage-specific manner during anther development. Messenger RNA was detected when tobacco bud sizes were between 0.5 cm and 1.0 cm. The appearance of mRNA coincided with meiosis and became undetectable at later stages of anther development. The reverse polymerase chain reaction (RT-PCR) amplification assay using isoform-specific primers showed that both of the CCaMK mRNAs were expressed in anther with similar expression patterns. The CCaMK protein expressed in Escherichia coli showed Ca2+-dependent autophosphorylation and Ca2+/calmodulin-dependent substrate phosphorylation. Calmodulin isoforms (PCM1 and PCM6) had differential effects on the regulation of autophosphorylation and substrate phosphorylation of tobacco CCaMK, but not lily CCaMK. The evolutionary tree of plant serine/threonine protein kinases revealed that calmodulin-dependent kinases form one subgroup that is distinctly different from Ca2+-dependent protein kinases (CDPKs) and other serine/threonine kinases in plants.

NASA Discipline Plant Biology↗
NASA NTRS1996

Molecular analysis and comparison of radiation-induced large deletions of the HPRT locus in primary human skin fibroblasts

Genetic alterations in gamma-ray- and alpha-particle-induced HPRT mutants were examined by multiplex polymerase chain reaction (PCR) analysis. A total of 39-63% of gamma-ray-induced and 31-57% of alpha-particle-induced mutants had partial or total deletions of the HPRT gene. The proportion of these deletion events was dependent on radiation dose, and at the resolution limits employed there were no significant differences between the spectra induced by equitoxic doses of alpha particles (0.2-0.4 Gy) and gamma rays (3 Gy). The molecular nature of the deletions was analyzed by the use of sequence tagged site (STS) primers and PCR amplification as a "probe" for specific regions of the human X chromosome within the Xq26 region. These STSs were closely linked and spanned regions approximately 1.7 Mbp from the telomeric side and 1.7 Mbp from the centromeric side of the HPRT gene. These markers include: DXS53, 299R, DXS79, yH3L, 3/19, PR1, PR25, H2, yH3R, 1/44, 1/67, 1/1, DXS86, D8C6, DXS10 and DXS144. STS analyses indicated that the maximum size of total deletions in radiation-induced HPRT mutants can be greater than 2.7 Mbp and deletion size appears to be dependent on radiation dose. There were no apparent differences in the sizes of the deletions induced by alpha particles or gamma rays. On the other hand, deletions containing portions of the HPRT gene were observed to be 800 kbp or less, and the pattern of the partial deletion induced by alpha particles appeared to be different from that induced by gamma rays.

NASA Discipline Radiation Health↗
NASA NTRS2009

Very-Long-Distance Remote Hearing and Vibrometry

A proposed development of laser-based instrumentation systems would extend the art of laser Doppler vibrometry beyond the prior limits of laser-assisted remote hearing and industrial vibrometry for detecting defects in operating mechanisms. A system according to the proposal could covertly measure vibrations of objects at distances as large as thousands of kilometers and could process the measurement data to enable recognition of vibrations characteristic of specific objects of interest, thereby enabling recognition of the objects themselves. A typical system as envisioned would be placed in orbit around the Earth for use as a means of determining whether certain objects on or under the ground are of interest as potential military targets. Terrestrial versions of these instruments designed for airborne or land- or sea-based operation could be similarly useful for military or law-enforcement purposes. Prior laser-based remote-hearing systems are not capable of either covert operation or detecting signals beyond modest distances when operated at realistic laser power levels. The performances of prior systems for recognition of objects by remote vibrometry are limited by low signal-to-noise ratios and lack of filtering of optical signals returned from targets. The proposed development would overcome these limitations. A system as proposed would include a narrow-band laser as its target illuminator, a lock-in-detection receiver subsystem, and a laser-power-control subsystem that would utilize feedback of the intensity of background illumination of the target to adjust the laser power. The laser power would be set at a level high enough to enable the desired measurements but below the threshold of detectability by an imaginary typical modern photodetector located at the target and there exposed to the background illumination. The laser beam would be focused tightly on the distant target, such that the receiving optics would be exposed to only one speckle. The return signal would be extremely-narrow-band filtered (to sub-kilohertz bandwidth) in the optical domain by a whispering-gallery- mode filter so as to remove most of the background illumination. The filtered optical signal would be optically amplified. This combination of optical filtering and optical amplification would provide an optical signal that would be strong enough to be detectable but not so strong as to saturate the detector in the lock-in detection subsystem.

Maleki, Lute↗
NASA NTRS1996

Evaluation of high temperature superconductive thermal bridges for space borne cryogenic detectors

Infrared sensor satellites are used to monitor the conditions in the earth's upper atmosphere. In these systems, the electronic links connecting the cryogenically cooled infrared detectors to the significantly warmer amplification electronics act as thermal bridges and, consequently, the mission lifetimes of the satellites are limited due to cryogenic evaporation. High-temperature superconductor (HTS) materials have been proposed by researchers at the National Aeronautics and Space Administration Langley's Research Center (NASA-LaRC) as an alternative to the currently used manganin wires for electrical connection. The potential for using HTS films as thermal bridges has provided the motivation for the design and the analysis of a spaceflight experiment to evaluate the performance of this superconductive technology in the space environment. The initial efforts were focused on the preliminary design of the experimental system which allows for the quantitative comparison of superconductive leads with manganin leads, and on the thermal conduction modeling of the proposed system. Most of the HTS materials were indicated to be potential replacements for the manganin wires. In the continuation of this multi-year research, the objectives of this study were to evaluate the sources of heat transfer on the thermal bridges that have been neglected in the preliminary conductive model and then to develop a methodology for the estimation of the thermal conductivities of the HTS thermal bridges in space. The Joule heating created by the electrical current through the manganin wires was incorporated as a volumetric heat source into the manganin conductive model. The radiative heat source on the HTS thermal bridges was determined by performing a separate radiant interchange analysis within a high-T(sub c) superconductor housing area. Both heat sources indicated no significant contribution on the cryogenic heat load, which validates the results obtained in the preliminary conduction model. A methodology was presented for the estimation of the thermal conductivities of the individual HTS thermal bridge materials and the effective thermal conductivities of the composite HTS thermal bridges as functions of temperature. This methodology included a sensitivity analysis and the demonstration of the estimation procedure using simulated data with added random errors. The thermal conductivities could not be estimated as functions of temperature; thus the effective thermal conductivities of the HTS thermal bridges were analyzed as constants.

Scott, Elaine P.↗
NASA NTRS2004

Phylogenetic and physiological diversity of microorganisms isolated from a deep greenland glacier ice core

We studied a sample from the GISP 2 (Greenland Ice Sheet Project) ice core to determine the diversity and survival of microorganisms trapped in the ice at least 120,000 years ago. Previously, we examined the phylogenetic relationships among 16S ribosomal DNA (rDNA) sequences in a clone library obtained by PCR amplification from genomic DNA extracted from anaerobic enrichments. Here we report the isolation of nearly 800 aerobic organisms that were grouped by morphology and amplified rDNA restriction analysis patterns to select isolates for further study. The phylogenetic analyses of 56 representative rDNA sequences showed that the isolates belonged to four major phylogenetic groups: the high-G+C gram-positives, low-G+C gram-positives, Proteobacteria, and the Cytophaga-Flavobacterium-Bacteroides group. The most abundant and diverse isolates were within the high-G+C gram-positive cluster that had not been represented in the clone library. The Jukes-Cantor evolutionary distance matrix results suggested that at least 7 isolates represent new species within characterized genera and that 49 are different strains of known species. The isolates were further categorized based on the isolation conditions, temperature range for growth, enzyme activity, antibiotic resistance, presence of plasmids, and strain-specific genomic variations. A significant observation with implications for the development of novel and more effective cultivation methods was that preliminary incubation in anaerobic and aerobic liquid prior to plating on agar media greatly increased the recovery of CFU from the ice core sample.

Bacteria/classification/genetics/isolation & purif↗
DOE OSTI2025

Quantifying the impacts of atmospheric rivers on the surface energy budget of the Arctic based on reanalysis

We present a comprehensive analysis of Arctic surface energy budget (SEB) components during atmospheric river (AR) events identified by integrated water vapor transport exceeding the monthly 85th-percentile climatological threshold in 3-hourly ERA5 reanalysis data from January 1980 to December 2019. Analysis of average anomalies in SEB components, net SEB, and the overall AR contribution to both the seasonal SEB components and net SEB climatology reveals clear seasonality and distinct land–sea–sea ice contrast patterns. Over the sea-ice-covered central Arctic Ocean, ARs significantly impact net SEB, inducing substantial surface warming in fall, winter, and spring. This warming is primarily driven by large anomalies in surface downward longwave radiation (LWD), which average 29–45 W m −2 during the cold seasons. In contrast, AR-related LWD anomalies are smaller in summer, averaging around 15 W m −2 , indicating a reduced impact during this season. Over sub-polar oceans, ARs have the most substantial positive impact on net SEB in cold seasons, mainly attributed to significant positive turbulent heat flux anomalies. AR-related turbulent heat anomalies reduce the upward turbulent flux, contributing up to −11 % relative to its seasonal climatology. In summer, ARs induce negative impacts on net SEB, primarily due to reduced shortwave radiation from increased cloud cover during AR events. Over continents, ARs generate smaller absolute impacts on net SEB because the large LWD anomalies are largely offset by corresponding increases in upward longwave radiation, particularly during cold seasons. Additionally, the seemingly large relative contributions of ARs to the net SEB over land primarily reflects the small magnitude of the climatological net SEB over continents. Greenland, especially western Greenland, exhibits significant downward longwave radiation anomalies associated with ARs, which drive large net SEB anomalies and contribute >54 % to mean SEB and induce amplified surface warming year-round. This holds significance for melt events, particularly during summer. Additionally, results of AR-related SEB impacts strongly depend on detection methods, as restrictive AR detection algorithms that emphasize extreme AR events, with large AR-related anomalies, do not necessarily indicate a large overall contribution to the SEB climatology due to the low occurrence frequency of these events. This study quantifies the role of ARs in the surface energy budget, contributing to our understanding of the Arctic warming and sea ice decline in ongoing Arctic amplification.

Arctic Sea ice change↗
NASA NTRS1999

A molecular description of the evolution of resistance

BACKGROUND: In vitro evolution has been used to obtain nucleic acid molecules with interesting functional properties. The evolution process usually is carried out in a stepwise manner, involving successive rounds of selection, amplification and mutation. Recently, a continuous in vitro evolution system was devised for RNAs that catalyze the ligation of oligonucleotide substrates, allowing the evolution of catalytic function to be studied in real time. RESULTS: Continuous in vitro evolution of an RNA ligase ribozyme was carried out in the presence of a DNA enzyme that was capable of cleaving, and thereby inactivating, the ribozyme. The DNA concentration was increased steadily over 33.5 hours of evolution, reaching a final concentration that would have been sufficient to inactivate the starting population in one second. The evolved population of ribozymes developed resistance to the DNA enzyme, reducing their vulnerability to cleavage by 2000-fold but retaining their own catalytic function. Based on sequencing and kinetic analysis of the ribozymes, two mechanisms are proposed for this resistance. One involves three nucleotide substitutions, together with two compensatory mutations, that alter the site at which the DNA enzyme binds the ribozyme. The other involves enhancement of the ribozyme's ability to bind its own substrate in a way that protects it from cleavage by the DNA enzyme. CONCLUSIONS: The ability to direct the evolution of an enzyme's biochemical properties in response to the behavior of another macromolecule provides insight into the evolution of resistance and may be useful in developing enzymes with novel or enhanced function.

Non-NASA Center↗
NASA NTRS2001

Micromachined TWTs for THz Radiation Sources

The Terahertz (THz) region of the electromagnetic spectrum (about 300 - 3000 GHz in frequency or about 0.1 - 1 mm free space wavelength) has enormous potential for high-data-rate communications, spectroscopy, astronomy, space research, medicine, biology, surveillance, remote sensing, industrial process control, etc. It has been characterized as the most scientifically rich, yet under-utilized, region of the electromagnetic spectrum. The most critical roadblock to full exploitation of the THz band is lack of coherent radiation sources that are powerful (0.001 - 1.0 W continuous wave), efficient (> 1%), frequency agile (instantaneously tunable over 1% bandwidths or more), reliable, and comparatively inexpensive. To develop vacuum electron device (VED) radiation sources satisfying these requirements, fabrication and packaging approaches must be heavily considered to minimize costs, in addition to the basic interaction physics and circuit design. To minimize size of the prime power supply, beam voltage must be minimized, preferably 10 kV. Solid state sources satisfy the low voltage requirement, but are many orders of magnitude below power, efficiency, and bandwidth requirements. On the other hand, typical fast-wave VED sources in this regime (e.g., gyrotrons, FELs) tend to be large, expensive, high voltage and very high power devices unsuitable for most of the applications cited above. VEDs based on grating or inter-digital (ID) circuits have been researched and developed. However, achieving forward-wave amplifier operation with instantaneous fractional bandwidths > 1% is problematic for these devices with low-energy (< 15 kV) electron beams. Moreover, the interaction impedance is quite low unless the beam-circuit spacing is kept particularly narrow, often leading to significant beam interception. One solution to satisfy the THz source requirements mentioned above is to develop micromachined VEDs, or "micro-VEDs". Among other benefits, micro-machining technologies provide superior high frequency wall conductivity as a result of superior surface smoothness compared with conventional mechanical or electric discharge machining approaches. Micro-VED technologies are already being applied to the development of millimeter-wave klystrons at Stanford Linear Accelerator Center and submillimeter-wave klystrons at the University of Leeds. We are investigating the use of micro-machining technologies to develop THz regime TWTs, with emphasis on folded-waveguide TWTs. The folded-waveguide TWT (FW-TWT) has several features that make it attractive for THz-regime micro-VED applications. It is a relatively simple circuit to design and fabricate, it is amenable to precision pattern replication by micro-machining, and it is has been demonstrated capable of forward-wave amplification with appreciable bandwidth. We are conducting experimental and computational studies of micro-VED FW-TWTs to examine their feasibility for applications at frequencies from 200 - 1000 GHz.

Booske, John H.↗
DOE OSTI2025

Prototype acoustic positioning system for the Pacific Ocean Neutrino Experiment

We present the design and initial performance characterization of the prototype acoustic positioning system intended for the Pacific Ocean Neutrino Experiment. It comprises novel piezo-acoustic receivers with dedicated filtering- and amplification electronics installed in P-ONE instruments and is complemented by a commercial system comprised of cabled and autonomous acoustic pingers for sub-sea installation manufactured by Sonardyne Ltd. We performed an in-depth characterization of the acoustic receiver electronics and their acoustic sensitivity when integrated into P-ONE pressure housings. These show absolute sensitivities of up to -125 dB re V2/μPa2 in a frequency range of 10–40 kHz. We furthermore conducted a positioning measurement campaign in the ocean by deploying three autonomous acoustic pingers on the seafloor, as well as a cabled acoustic interrogator and a P-ONE prototype module deployed from a ship. Using a simple peak-finding detection algorithm, we observe high accuracy in the tracking of relative ranging times at approximately 230–280 μs at distances of up to 1600 m, which is sufficient for positioning detectors in a cubic-kilometer detector and which can be further improved with more involved detection algorithms. The tracking accuracy is further confirmed by independent ranging of the Sonardyne system and closely follows the ship's drift in the wind measured by GPS. The absolute positioning shows the same tracking accuracy with its absolute precision only limited by the large uncertainties of the deployed pinger positions on the seafloor.

Data analysis↗