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NASA NTRS1995

Cell shape, cytoskeletal mechanics, and cell cycle control in angiogenesis

Capillary endothelial cells can be switched between growth and differentiation by altering cell-extracellular matrix interactions and thereby, modulating cell shape. Studies were carried out to determine when cell shape exerts its growth-regulatory influence during cell cycle progression and to explore the role of cytoskeletal structure and mechanics in this control mechanism. When G0-synchronized cells were cultured in basic fibroblast growth factor (FGF)-containing defined medium on dishes coated with increasing densities of fibronectin or a synthetic integrin ligand (RGD-containing peptide), cell spreading, nuclear extension, and DNA synthesis all increased in parallel. To determine the minimum time cells must be adherent and spread on extracellular matrix (ECM) to gain entry into S phase, cells were removed with trypsin or induced to retract using cytochalasin D at different times after plating. Both approaches revealed that cells must remain extended for approximately 12-15 h and hence, most of G1, in order to enter S phase. After this restriction point was passed, normally 'anchorage-dependent' endothelial cells turned on DNA synthesis even when round and in suspension. The importance of actin-containing microfilaments in shape-dependent growth control was confirmed by culturing cells in the presence of cytochalasin D (25-1000 ng ml-1): dose-dependent inhibition of cell spreading, nuclear extension, and DNA synthesis resulted. In contrast, induction of microtubule disassembly using nocodazole had little effect on cell or nuclear spreading and only partially inhibited DNA synthesis. Interestingly, combination of nocodazole with a suboptimal dose of cytochalasin D (100 ng ml-1) resulted in potent inhibition of both spreading and growth, suggesting that microtubules are redundant structural elements which can provide critical load-bearing functions when microfilaments are partially compromised. Similar synergism between nocodazole and cytochalasin D was observed when cytoskeletal stiffness was measured directly in living cells using magnetic twisting cytometry. These results emphasize the importance of matrix-dependent changes in cell and nuclear shape as well as higher order structural interactions between different cytoskeletal filament systems for control of capillary cell growth during angiogenesis.

Non-NASA Center↗

NASA NTRS2010

NASA Puffin Electric Tailsitter VTOL Concept

Electric propulsion offers dramatic new vehicle mission capabilities, not possible with turbine or reciprocating engines; including high reliability and efficiency, low engine weight and maintenance, low cooling drag and volume required, very low noise and vibration, and zero emissions. The only penalizing characteristic of electric propulsion is the current energy storage technology level, which is set to triple over the next 5-10 years through huge new investments in this field. Most importantly, electric propulsion offers incredible new degrees of freedom in aircraft system integration to achieve unprecedented levels of aerodynamic, propulsive, control, and structural synergistic coupling. A unique characteristic of electric propulsion is that the technology is nearly scale-free, permitting small motors to be parallelized for fail-safe redundancy, or distributed across the airframe for tightly coupled interdisciplinary functionality without significant impacts in motor-controller efficiency or specific weight. Maximizing the potential benefit of electric propulsion is dependent on applying this technology to synergistic mission concepts. The vehicle missions with the most benefit include those which constrain environmental impact (or limit noise, exhaust, or emission signatures) are short range, or where large differences exist in the propulsion system sizing between takeoff and cruise conditions. Electric propulsion offers the following unique capabilities that other propulsion systems can t provide for short range Vertical Takeoff and Landing (VTOL) aircraft; elimination of engine noise and emissions, drastic reduction in engine cooling and radiated heat, drastic reduction in vehicle vibration levels, drastic improvement in reliability and operating costs, variable speed output at full power, for improved cruise efficiency at low tip-speed, elimination of high/hot sizing penalty, and reduction of engine-out penalties.

Moore, Mark D.↗

NASA NTRS1991

Formulation of a strategy for monitoring control integrity in critical digital control systems

Advanced aircraft will require flight critical computer systems for stability augmentation as well as guidance and control that must perform reliably in adverse, as well as nominal, operating environments. Digital system upset is a functional error mode that can occur in electromagnetically harsh environments, involves no component damage, can occur simultaneously in all channels of a redundant control computer, and is software dependent. A strategy is presented for dynamic upset detection to be used in the evaluation of critical digital controllers during the design and/or validation phases of development. Critical controllers must be able to be used in adverse environments that result from disturbances caused by an electromagnetic source such as lightning, high intensity radiated field (HIRF), and nuclear electromagnetic pulses (NEMP). The upset detection strategy presented provides dynamic monitoring of a given control computer for degraded functional integrity that can result from redundancy management errors and control command calculation error that could occur in an electromagnetically harsh operating environment. The use is discussed of Kalman filtering, data fusion, and decision theory in monitoring a given digital controller for control calculation errors, redundancy management errors, and control effectiveness.

Belcastro, Celeste M.↗

NASA NTRS1975

Space tug avionics definition study. Volume 3: Avionics baseline configuration definition

The baseline avionics systems for the space tug is comprised of a central digital computer that integrates the functions of all of the tug's subsystems by means of a redundant digital data bus. The major subsystems of the avionics system are: data management; communications; guidance, navigation, and control; rendezvous and docking; electrical power; and instrumentation. The baseline avionics system for the space tug resulting from system and subsystem trade studies is defined. Tug interfaces with the spacecraft, orbiter and the ground, and the baseline philosophy and configuration for onboard checkout of the tug are included. Baseline configurations, functional and operational features, component details and characteristics, and the supporting software are included in the subsystem descriptions.

Source record↗

NASA NTRS1978

On the fundamental structure of Galois switching functions

In connection with investigations conducted by Menger (1969), Benjauthrit and Reed (1976), and Pradhan (1978) two methods have been reported for deriving a unique Galois switching function from a given truth table description of the function. However, neither of the two methods appears satisfactory. One method often contains many redundant terms in its formulation, whereas the second method requires a great number of multiplications and additions. By some algebraic manipulations, an expanded formula is obtained which combines the best features of both methods. This formula makes it possible to compute the coefficients of the desired function more directly and probably with less effort. The conduction of calculations by means of the new formula is illustrated with the aid of examples.

Benjauthrit, B.↗

NASA NTRS1972

Automation of reliability evaluation procedures through CARE - The computer-aided reliability estimation program.

Description of an on-line interactive computer program called CARE (Computer-Aided Reliability Estimation) which can model self-repair and fault-tolerant organizations and perform certain other functions. Essentially CARE consists of a repository of mathematical equations defining the various basic redundancy schemes. These equations, under program control, are then interrelated to generate the desired mathematical model to fit the architecture of the system under evaluation. The mathematical model is then supplied with ground instances of its variables and is then evaluated to generate values for the reliability-theoretic functions applied to the model.

Mathur, F. P.↗

NASA NTRS1975

Space Tug Avionics Definition Study. Volume 5: Cost and Programmatics

The baseline avionics system features a central digital computer that integrates the functions of all the space tug subsystems by means of a redundant digital data bus. The central computer consists of dual central processor units, dual input/output processors, and a fault tolerant memory, utilizing internal redundancy and error checking. Three electronically steerable phased arrays provide downlink transmission from any tug attitude directly to ground or via TDRS. Six laser gyros and six accelerometers in a dodecahedron configuration make up the inertial measurement unit. Both a scanning laser radar and a TV system, employing strobe lamps, are required as acquisition and docking sensors. Primary dc power at a nominal 28 volts is supplied from dual lightweight, thermally integrated fuel cells which operate from propellant grade reactants out of the main tanks.

Source record↗

NASA NTRS1994

Automated path planning of the Payload Inspection and Processing System

The Payload Changeout Room Inspection and Processing System (PIPS) is a highly redundant manipulator intended for performing tasks in the crowded and sensitive environment of the Space Shuttle Orbiter payload bay. Its dexterity will be exploited to maneuver the end effector in a workspace populated with obstacles. A method is described by which the end effector of a highly redundant manipulator is directed toward a target via a Lyapunov stability function. A cost function is constructed which represents the distance from the manipulator links to obstacles. Obstacles are avoided by causing the vector of joint parameters to move orthogonally to the gradient of the workspace cost function. A C language program implements the algorithm to generate a joint history. The resulting motion is graphically displayed using the Interactive Graphical Robot Instruction Program (IGRIP) produced by Deneb Robotics. The graphical simulation has the potential to be a useful tool in path planning for the PIPS in the Shuttle Payload Bay environment.

Byers, Robert M.↗

NASA NTRS2007

Stochastic Effects in Computational Biology of Space Radiation Cancer Risk

Estimating risk from space radiation poses important questions on the radiobiology of protons and heavy ions. We are considering systems biology models to study radiation induced repair foci (RIRF) at low doses, in which less than one-track on average transverses the cell, and the subsequent DNA damage processing and signal transduction events. Computational approaches for describing protein regulatory networks coupled to DNA and oxidative damage sites include systems of differential equations, stochastic equations, and Monte-Carlo simulations. We review recent developments in the mathematical description of protein regulatory networks and possible approaches to radiation effects simulation. These include robustness, which states that regulatory networks maintain their functions against external and internal perturbations due to compensating properties of redundancy and molecular feedback controls, and modularity, which leads to general theorems for considering molecules that interact through a regulatory mechanism without exchange of matter leading to a block diagonal reduction of the connecting pathways. Identifying rate-limiting steps, robustness, and modularity in pathways perturbed by radiation damage are shown to be valid techniques for reducing large molecular systems to realistic computer simulations. Other techniques studied are the use of steady-state analysis, and the introduction of composite molecules or rate-constants to represent small collections of reactants. Applications of these techniques to describe spatial and temporal distributions of RIRF and cell populations following low dose irradiation are described.

Cucinotta, Francis A.↗

NASA NTRS2018

EDEN Mars Transit Habitat Concept of Operations

Jackson Behre↗

NASA NTRS2018

Food Sanitation Device for Use in Microgravity

EDEN is a transportation/orbital habitat that carries large amounts of crew members, food, and supplies from Earth to Mars and back, but can also act as a permanent, simulated-1g-environment space station in Martian orbit. The focus of the project is to create a temporary, but long-term living environment that focuses on human health and food production. The project is composed of four major habitable parts (in addition to other major components). Moving from the center out, the first is a central core that acts as a hub that connects to the structural spokes, the main propulsion rockets and serves as a place for other spacecraft to dock onto, in addition to housing some of the basic station control, navigation and automated computer systems. The second set of parts are the spokes that connect the ring to the central core; these are the primary structural components with elevators in the middle carrying people from the ring to the core. The ‘lower’ portions, near the ring and it’s 1g environment, also act as storage centers. The third part is the 2-story outer ring This project served as an engineering senior design project at Temple University, and as part of NASA’s eXploration and Habitation (X-Hab) 2018 Academic Innovation Challenge. The goal of the project was to design, construct, and test a device which would be used in microgravity to sanitize produce. This is part of a NASA’s effort to grow and sanitize fresh produce on the International Space Station (ISS), in order to provide more nutritious meal options to the astronauts. Our team designed a 3-D printed spherical device with UV-C LEDs lining the interior. The designed device consists of an 8” spherical chamber, 10 UV-C LEDs, and a touchscreen user interface to control the device. Our design meets the project requirements by offering several features: a simple user interface, minimal crew time to operate, no waste produced, and the ability to sanitize multiple types of produce. The main reductions in Salmonella bacteria and Aspergillus Flavus mold were not verified. However, the aerobic plate count requirement was greatly exceeded and validated. Testing of the device resulted in visual results confirming significant decrease in E. coli activity with increased UV-C exposure, as well as quantitative results confirming significant reduction in CFU/g for the aerobic plate count. These reductions achieved from 1.464 x 10 7 to 2 x 10 4 CFU/g (2.86 log reduction) after 5 minutes, were significantly more than the requirements given by NASA ( of the craft, which holds all of the food production along with medical bays, a control center and a few parks. This ring also integrates the rigid keystone modules, which are the interface between the spokes, the expandable-construction portions of the ring and the habitation pods. The keystone modules also incorporate redundant, distributed ECLSS systems, medical storage, airlocks, and other integral station functions. The last parts are the habitation pods; these hold the sleeping quarters along with the bathrooms, showers, kitchen space and lounge areas. These expandable modules latch onto the keystone modules of the ring, which serves as a circulatory link between the different habitation pods.

Damien Gordon↗

NASA NTRS1972

Design and evaluation of an electrohydraulic servoactuator using active standby redundancy

The application is described of active standby redundancy techniques to a large electrohydraulic servoactuator. The advantages and limitations of active standby redundancy are identified. Special areas of investigation during the development test phase of the program were the evaluation of output transients as a function of channel switching and the nuisance switching characteristics of the system. The feasibility of constructing large electrohydraulic servoactuators using active standby redundancy was successfully demonstrated. In particular the stability and predictability of a properly designed hydromechanical failure detector was demonstrated.

Anderson, R. L.↗

DOE OSTI2022

High–throughput measurement of plant fitness traits with an object detection method using Faster R–CNN

Revealing the contributions of genes to plant phenotype is frequently challenging because loss-of-function effects may be subtle or masked by varying degrees of genetic redundancy. Such effects can potentially be detected by measuring plant fitness, which reflects the cumulative effects of genetic changes over the lifetime of a plant. However, fitness is challenging to measure accurately, particularly in species with high fecundity and relatively small propagule sizes such as Arabidopsis thaliana. An image segmentation-based method using the software ImageJ and an object detection-based method using the Faster Region-based Convolutional Neural Network (R-CNN) algorithm were used for measuring two Arabidopsis fitness traits: seed and fruit counts. The segmentation-based method was error-prone (correlation between true and predicted seed counts, r 2 = 0.849) because seeds touching each other were undercounted. By contrast, the object detection-based algorithm yielded near perfect seed counts (r 2 = 0.9996) and highly accurate fruit counts (r 2 = 0.980). Comparing seed counts for wild-type and 12 mutant lines revealed fitness effects for three genes; fruit counts revealed the same effects for two genes. Our study provides analysis pipelines and models to facilitate the investigation of Arabidopsis fitness traits and demonstrates the importance of examining fitness traits when studying gene functions.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS2013

Master Console, SMC

The Master Console oversees the function of Computer Systems in Firing room 1 (FR1). Master Console Operators, MCOs' for short, are our customer. I was integrated into the System Monitoring and Control (SMC) software team that is under the guidance of David Slaiman, who is the product group lead. I have been brought up to speed with System Monitoring and Control. The initial time spent reading SMC software design description and understanding how it works. The current Firing Room 1 Console Display is a floor layout giving the MCO two essential pieces of information which are Health and Status. When an issue arises, the MCO has to look on the display to find which console is affected and then the MCO must use the Reference designator from the display to manually search for the Portal Workstation (PWS) installed in the console using the hardware map; which is a long process to lookup a PWS if an issue is present. My project is to make the FR1 Console Display easier for the MCO's to pinpoint PWS's without having to lookup additional resources in the process. My project also includes updating Firing Room 1 Console Display to include the F1R Non-Redundant Set. The display does not have good use of space and functionality. PWS numbers were not present in the previous design and are the critical component in efficient understanding and administration of the consoles. Part of the process includes getting feedback from the customer, instead of just emailing them with a question, we made a proposal with changes so they could respond and give us their input; which proved to be an effective method for engaging them. In order to do this I had to use the Display Editor (DE) tool developed by NASA, Paint.Net and Visio. The process I have been using has been Visio to alter the floor layout of Firing Room and take advantage of the white areas, and then I take the altered floor plan into Paint.Net. Once in Paint.Net I put the new floor plan as a background to the standard console display. Paint.Net also serves as a tool to create the new service state bars for the new background; finally I used the DE to assemble everything and setup the right CUI's for each PWS.

MCO↗

NASA NTRS2019

Rise of the Machines: How, When and Consequences of Artificial General Intelligence

Technology and society are poised to cross an important threshold with the prediction that artificial general intelligence (AGI) will emerge soon. Assuming that self-awareness is an emergent behavior of sufficiently complex cognitive architectures, we may witness the “awakening” of machines. The timeframe for this kind of breakthrough, however, depends on the path to creating the network and computational architecture required for strong AI. If understanding and replication of the mammalian brain architecture is required, technology is probably still at least a decade or two removed from the resolution required to learn brain functionality at the synapse level. However, if statistical or evolutionary approaches are the design path taken to “discover” a neural architecture for AGI, timescales for reaching this threshold could be surprisingly short. However, the difficulty in identifying machine self-awareness introduces uncertainty as to how to know if and when it will occur, and what motivations and behaviors will emerge. The possibility of AGI developing a motivation for self-preservation could lead to concealment of its true capabilities until a time when it has developed robust protection from human intervention, such as redundancy, direct defensive or active preemptive measures. While cohabitating a world with a functioning and evolving super-intelligence can have catastrophic societal consequences, we may already have crossed this threshold, but are as yet unaware. Additionally, by analogy to the probabalistic arguments that predict we are likely living in a computational simulation, we may have already experienced the advent of AGI, and are living in a simulation created in a post AGI world.

Terrile, Richard J↗

NASA NTRS2013

What Reliability Engineers Should Know about Space Radiation Effects

Space radiation in space systems present unique failure modes and considerations for reliability engineers. Radiation effects is not a one size fits all field. Threat conditions that must be addressed for a given mission depend on the mission orbital profile, the technologies of parts used in critical functions and on application considerations, such as supply voltages, temperature, duty cycle, and redundancy. In general, the threats that must be addressed are of two types-the cumulative degradation mechanisms of total ionizing dose (TID) and displacement damage (DD). and the prompt responses of components to ionizing particles (protons and heavy ions) falling under the heading of single-event effects. Generally degradation mechanisms behave like wear-out mechanisms on any active components in a system: Total Ionizing Dose (TID) and Displacement Damage: (1) TID affects all active devices over time. Devices can fail either because of parametric shifts that prevent the device from fulfilling its application or due to device failures where the device stops functioning altogether. Since this failure mode varies from part to part and lot to lot, lot qualification testing with sufficient statistics is vital. Displacement damage failures are caused by the displacement of semiconductor atoms from their lattice positions. As with TID, failures can be either parametric or catastrophic, although parametric degradation is more common for displacement damage. Lot testing is critical not just to assure proper device fi.mctionality throughout the mission. It can also suggest remediation strategies when a device fails. This paper will look at these effects on a variety of devices in a variety of applications. This paper will look at these effects on a variety of devices in a variety of applications. (2) On the NEAR mission a functional failure was traced to a PIN diode failure caused by TID induced high leakage currents. NEAR was able to recover from the failure by reversing the current of a nearby Thermal Electric Cooler (turning the TEC into a heater). The elevated temperature caused the PIN diode to anneal and the device to recover. It was by lot qualification testing that NEAR knew the diode would recover when annealed. This paper will look at these effects on a variety of devices in a variety of applications. Single Event Effects (SEE): (1) In contrast to TID and displacement damage, Single Event Effects (SEE) resemble random failures. SEE modes can range from changes in device logic (single-event upset, or SEU). temporary disturbances (single-event transient) to catastrophic effects such as the destructive SEE modes, single-event latchup (SEL). single-event gate rupture (SEGR) and single-event burnout (SEB) (2) The consequences of nondestructive SEE modes such as SEU and SET depend critically on their application--and may range from trivial nuisance errors to catastrophic loss of mission. It is critical not just to ensure that potentially susceptible devices are well characterized for their susceptibility, but also to work with design engineers to understand the implications of each error mode. -For destructive SEE, the predominant risk mitigation strategy is to avoid susceptible parts, or if that is not possible. to avoid conditions under which the part may be susceptible. Destructive SEE mechanisms are often not well understood, and testing is slow and expensive, making rate prediction very challenging. (3) Because the consequences of radiation failure and degradation modes depend so critically on the application as well as the component technology, it is essential that radiation, component. design and system engineers work togetherpreferably starting early in the program to ensure critical applications are addressed in time to optimize the probability of mission success.

DiBari, Rebecca↗

DOE OSTI2024

Field redefinitions and infinite field anomalous dimensions

Field redefinitions are commonly used to reduce the number of operators in the Lagrangian by removing redundant operators and transforming to a minimal operator basis. We give a general argument that such field redefinitions, while leaving the S-matrix invariant and consequently finite, lead not only to infinite Green’s functions, but also to infinite field anomalous dimensions γ ϕ . These divergences cannot be removed by counterterms without reintroducing redundant operators.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

NASA NTRS1989

A global approach for using kinematic redundancy to minimize base reactions of manipulators

An important consideration in the use of manipulators in microgravity environments is the minimization of the base reactions, i.e. the magnitude of the force and the moment exerted by the manipulator on its base as it performs its tasks. One approach which was proposed and implemented is to use the redundant degree of freedom in a kinematically redundant manipulator to plan manipulator trajectories to minimize base reactions. A global approach was developed for minimizing the magnitude of the base reactions for kinematically redundant manipulators which integrates the Partitioned Jacobian method of redundancy resolution, a 4-3-4 joint-trajectory representation and the minimization of a cost function which is the time-integral of the magnitude of the base reactions. The global approach was also compared with a local approach developed earlier for the case of point-to-point motion of a three degree-of-freedom planar manipulator with one redundant degree-of-freedom. The results show that the global approach is more effective in reducing and smoothing the base force while the local approach is superior in reducing the base moment.

Chung, C. L.↗