Higher plant response to elevated ultraviolet irradiance
The effect of ultraviolet irradiation on pollen germination, ultraviolet action spectra influencing photosynthesis, and plant adaptations to ultraviolet-B radiation are reported.
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The effect of ultraviolet irradiation on pollen germination, ultraviolet action spectra influencing photosynthesis, and plant adaptations to ultraviolet-B radiation are reported.
The problem of pre-harvest sprouting of wheat is surveyed and a literature review of the effects of environmental conditions on pre-harvest sprouting is presenting. Physiological, biochemical, and morphological changes occurring within the wheat seed during germination, harvest, and storage are discussed. The effects of moisture, humidity, and temperature, particularly on seed dormancy, are considered. Procedures used in Europe for predicting the potential for sprouting are evaluated.
The genesis of lunar exploration programs is described. The idea that the dead moon could give important clues about the origin of the solar system germinated into plans for a soft landing on the moon and then into the Apollo program. The exchanges between NASA scientists and other astronomers that led to these plans are recounted.
The effects of weightlessness on three aspects of mammalian reproduction: oocyte development, fertilization, and early embryogenesis was studied. Zero-gravity conditions within the laboratory by construction of a clinostat designed to support in vitro tissue culture were simulated and the effects of simulated weightlessness on meiotic maturation in mammalian oocytes using mouse as the model system were studied. The timing and frequency of germinal vesicule breakdown and polar body extrusion, and the structural and numerical properties of meiotic chromosomes at Metaphase and Metaphase of meiosis are assessed.
The effects of long term exposure to the near Earth space environment on advanced electrooptical and radiation sensor components were examined. The effect of long duration spaceflight on the germination rate of selected terrestrial plant seeds is observed in exobiological experiments.
The Purdue University GAS-1 payload was flown on STS-7. Results of the three experiments included in the payload are summarized. The experiments conducted were: (1) the detection of nuclear particles in near-Earth space environment, (2) an investigation of the effect of microgravity on the germination of sunflower seeds, and (3) an examination of the motion of mercury under low gravity conditions. Results of the payload experiments are discussed from an engineering design standpoint. Data were obtained from one of the experiments, but electrical and mechanical malfunctions prevented the operation of the other two. The thermal control design maintained the desired temperatures and the structure successfully supported all components. The microprocessor collected and stored temperature readings and other data during the flight. A series of recommendations based on these results are included herein.
Student projects for the Get Away Special (GAS) space shuttle program were summarized. Experimental topics included: seed germination, shrimp growth, liquid lasers, planaria regeneration, fluid dynamics (wicking), soil molds, antibiotics, crystallization, the symbiosis of yeast and fungi, and the performance of electronic chips. A brief experimental design is included for each project.
A microgravity growth chamber was designed to investigate the phototropic response of radish seedlings. Enclosed in a one fourth inch thick, hexagonal, fiberglass-foam spacepak nineteen inches across corners, the experiment consists of a growth chamber and germination tray, a water reservoir and solenoid valve, a fluorescent light for photo simulation, a Minolta X700 camera with programmable back, a 50 mm macro lens and flash, a battery pack, and a computer controller. Two temperature sensors and one light sensor located in the walls of the growth chamber provide temperature and illumination data. A computer provides 8 K command and 34 K data storage capability. The experiment was not activated during the STS flight because a malfunctioning latching relay stuck and reduced the battery power level.
Tentatively scheduled to fly on STS-17 (41G), this get away special aims to demonstrate amateur radio transmissions to global ground stations in the English language. Experiments No. 1, 2, and 3 use the micro-gravity of space flight to study the solidification of lead-antimony and aluminum-copper alloys, the germination of radish seeds, and the growth of potassium-tetracyanoplatinate hydrate crystals in an aqueous solution. Flight results are to be compared with Earth-based data. Experiment No. 4 (the Marshall Amateur Radio Club Experiment - MARCE) features radio transmissions and also provides timing for the start of all other experiments. A microprocessor obtains real-time data from all experiments as well as temperature and pressure measurements within the GAS canister. These data are to be transmitted on previously announced amateur radio frequencies after they are converted into the English language by a digitalker for general reception. The support structure for the G #007 experiments consists of two primary plates and four bumper assemblies.
PROJECT EXPLORER is a program that will fly student-developed experiments onboard the Space Shuttle in NASA's Get-Away Special (GAS) containers. The program is co-sponsored by the Alabama Space and Rocket Center, the Alabama-Mississippi Section of the American Institute of Aeronautics and Astronautics, Alabama A&M University and requires extensive support by the University of Alabama in Huntsville. A unique feature of this project will demonstrate transmissions to ground stations on amateur radio frequencies in English language. Experiments Nos. 1, 2, and 3 use the microgravity of space flight to study the solidification of lead-antimony and aluminum-copper alloys, the growth of potassium-tetracyanoplatinate hydrate crystals in an aqueous solution, and the germination of radish seeds. Flight results will be compared with Earth-based data. Experiment No. 4 features radio transmission and will also provide timing for the start of all other experiments. A microprocessor will obtain real-time data from all experiments as well as temperature and pressure measurements taken inside the canister. These data will be transmitted on previously announced amateur radio frequencies after they have been converted into the English language by a digitalker for general reception.
F. Kaiser's germinal 1944 report on his 'resultant-height' concept, now known as energy modelling, is reviewed. The data base for the Me. 262 jet fighter is recreated via spline-lattice representation of specific excess power. Minimum-time and 'distance'-climb trajectories are generated in an attempt to check Kaiser's results. Agreement is good for the minimum-time calculations but only qualitative agreement is obtained for the mysterious 'distance climbs' whose documentation is fragmentary. The character of optimal climb-dash trajectories in energy approximation is examined and illustrated.
The effects of microgravity on meiosis, fertilization, and early embryonic development in mammals are being examined by using a clinostat to reorient the cells with respect to the gravity vector. A clinostat capable of supporting mammalian cells in tissue culture has been developed. Initial studies have focused on examining the effects of clinostat rotation on meiotic maturation in mouse oocytes. Oocytes recovered from ovarian follicles were subjected to clinostat rotation on a horizontal or vertical axis or to static conditions for a 16 hr period. No gross morphological changes and no effects on germinal vesicle breakdown were observed under any rotation conditions (1/4, 1, 10, 30, 100 RPM). Success of meiotic progression to Metaphase II was comparable among experimental and control groups except at 100 RPM, where a slight inhibition was observed.
Physiological and cytogenetic changes in the Welsh onion plants induced by a short (82 days) and long term (522 days) space flight are expressed in decrease of seed germination, inhibition of stem growth, depression of cell division in root meristem, and increase in the number of structural chromosome rearrangements. The treatment of such plants with solutions of a-tocopherol, auxin, and kinetin decreased the level of chromosome aberrations to the control one and normalized cell divisions and growth partly or completely.
Capillary-effect root-environment system (CERES) is experimental apparatus for growing plants in nutrient solutions. Solution circulated at slight tension in cavity filled with plastic screen and covered by porous plastic membrane. By adsorptive attraction, root draws solution through membrane. Conceived for use in microgravity of space, also finds terrestrial application in germinating seedlings, because it protects them from extremes of temperature, moisture, and soil pH and from overexposure to fertilizers and herbicides.
A series of tests were conducted to monitor atmospheric leakage rate and condensate production in NASA's Biomass Production Chamber (BPC). Water was circulated through the 64 plant culture trays inside the chamber during the tests but no plants were present. Environmental conditions were set to a 12-hr photoperiod with either a matching 26 C (light)/20 C (dark) thermoperiod, or a constant 23 C temperature. Leakage, as determined by carbon dioxide decay rates, averaged about 9.8 percent for the 26 C/20 C regime and 7.3 percent for the constant 23 C regime. Increasing the temperature from 20 C to 26 C caused a temporary increase in pressure (up to 0.5 kPa) relative to ambient, while decreasing the temperature caused a temporary decrease in pressure of similar magnitude. Little pressure change was observed during transition between 23 C (light) and 23 C (dark). The lack of large pressure events under isothermal conditions may explain the lower leakage rate observed. When only the plant support inserts were placed in the culture trays, condensate production averaged about 37 liters per day. Placing acrylic germination covers over the tops of culture trays reduced condensate production to about 7 liters per day. During both tests, condensate production from the lower air handling system was 60 to 70 percent greater than from the upper system, suggesting imbalances exist in chilled and hot water flows for the two air handling systems. Results indicate that atmospheric leakage rates are sufficiently low to measure CO2 exchange rates by plants and the accumulation of certain volatile contaminants (e.g., ethylene). Control system changes are recommended in order to balance operational differences (e.g., humidity and temperature) between the two halves of the chamber.
The main objectives of the Biostack Experiment are to study the effectiveness of the structured components of the cosmic radiation to bacterial spores, plant seeds, and animal cysts for a long duration spaceflight and to get dosimetric data such as particle fluences and spectra and total doses for the Long Duration Exposure Facility orbit. The configuration of the experiment packages allows the localization of the trajectory of the particles in each biological layer and to correlate the potential biological impairment or injury with the physical characteristics of the responsible particle. Although the Biostack Experiment was designed for a long duration flight of only nine months, most of the biological systems show a high hatching or germination rate. Some of the first observations are an increase of the mutation rate of embryonic lethals in the second generation of Arabidopsis seeds, somatic mutations, and a reduction of growth rates of corn plants and a reduction of life span of Artemia salina shrimps. The different passive detector systems are also in a good shape and give access to a proper dosimetric analysis. The results are summarized, and some aspects of future analysis are shown.
Micropropagation is currently used to clone fruits, nuts, and vegetables and involves controlling the outgrowth in vitro of basal, axillary, or adventitious buds. Following clonal multiplication, shoots are divided and rooted. This process has greatly reduced space and energy requirements in greenhouses and field nurseries and has increased multiplication rates by greater than 20 fold for some vegetatively propagated crops and breeding lines. Cereal and legume crops can also be cloned by tissue culture through somatic embryogenesis. Somatic embryos can be used to produce 'synthetic seed', which can tolerate desiccation and germinate upon rehydration. Synthetic seed of hybrid wheat, rice, soybean and other crops could be produced in a controlled ecological life support system. Thus, yield advantages of hybreds over inbreds (10 to 20 percent) could be exploited without having to provide additional facilities and energy for parental-line and hybrid seed nurseries.
The goal of Closed Ecological Life Support System (CELSS) studies is to examine the effects of microgravity on yield and quality of plant products and on the interactions between irradiance and crop area. Measuring yield and quality of crops as a function of irradiance in microgravity is virtually unique to the CELSS program, as is the emphasis on canopies rather than individual plants. The first step for space experiments is to develop a relatively stress free environment for plant growth, something that has so far never been achieved. High light levels are essential, and there must be time enough to complete a significant portion of the life cycle. Optimal atmosphere and nutrients must be provided. Such responses as germination, orientation of roots and shoots, photosynthesis and respiration, floral initiation and development, and seed maturation and viability will be studied.