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At least 271 records · Page 15

An Optical Index of Phytoplankton Photoacclimation and Its Relation to Light-Saturated Photosynthesis in the Sea

In relation to understanding ocean biology at the global scale, one of NASA's primary foci has been measurements of near-surface concentrations of phytoplankton chlorophyll. Chlorophyll is an important light-absorbing pigment in phytoplankton. The absorbed light energy is used to fix carbon in the process of photosynthesis. Photosynthesis, in turn, is critical to the growth of phytoplankton and the function of entire marine ecosystems. Thus, the use of satellite surface chlorophyll data to estimate primary production in the ocean has been a key focus of much biological oceanography research. One of the major challenges in this research is to develop relationships that allow a given chlorophyll concentration (a standing stock) to be interpreted in terms of carbon fixation (a rate). This problem centers on the description of the light-saturated photosynthetic rate, Pbmax. In this paper, we describe how optical measurements of light attenuation provide information on particulate organic carbon (POC) concentrations. We then show how the ratio of POC to chlorophyll (Theta) provides critical information on variability in Pbmax. We then test this relationship between Theta and Pbmax using field data from a variety of open ocean ecosystems.

Behrenfeld, Michael J.↗

Early quantitative method for measuring germination in non-green spores of Dryopteris paleacea using an epifluorescence-microscope technique

A method is described to determine germination by blue-light excited red fluorescence in the positively photoblastic spores of Dryopteris paleacea Sw. This fluorescence is due to chlorophyll as evidenced from 1) a fluorescence-emission spectrum in vivo, where a bright fluorescence around 675 nm is obtained only in red light (R)-irradiated spores and 2) in vitro measurements with acetone extracts prepared from homogenized spores. Significant amounts of chlorophyll can be found only in R-treated spores; this chlorophyll exhibits an emission band around 668 nm, when irradiated with 430 nm light at 21 degrees C. Compared to other criteria for germination, such as swelling of the cell, coat splitting, greening, and rhizoid formation, which require longer periods after induction for their expression, chlorophyll fluorescence can be used to quantify germination after two days. This result is confirmed by fluence-response curves for R-induced spore germination; the same relationship between applied R and germination is obtained by the evaluation with the epifluorescence method 2 days after the light treatment as compared with the evaluation with bright-field microscopy 5 days after the inducing R. Using this technique we show for the first time that Ca2+ contributes to the signal-transduction chain in phytochrome-mediated chlorophyll synthesis in spores of Dryopteris paleacea.

NASA Discipline Plant Biology↗

Root-shoot interaction in the greening of wheat seedlings grown under red light

Wheat seedlings grown with roots exposed to constant red light (300-500 micromoles m-2 s-1) did not accumulate chlorophyll in the leaves. In contrast, seedlings grown with their roots shielded from light accumulated chlorophylls. Chlorophyll biosynthesis could be induced in red-light-grown chlorophyll-deficient yellow plants by either reducing the red-light intensity at the root surface to 100 micromoles m-1 s-1 or supplementing with 6% blue light. The inhibition of chlorophyll biosynthesis was due to impairment of the Mg-chelatase enzyme working at the origin of the Mg-tetrapyrrole pathway. The root-perceived photomorphogenic inhibition of shoot greening demonstrates root-shoot interaction in the greening process.

NASA Center KSC↗

Apparent Optical Properties in Waters Influenced by the Mississippi River

In-water downwelling irradiance (E(sub d)) and upwelling radiance (L(sub u)) were measured in coastal waters influenced by the Mississippi River at wavelengths corresponding to SeaWiFS spectral bands in April of 2000. Results of derived apparent optical properties (AOP's) such as spectral diffise attenuation coefficient for downwelling irradiance (K(sub d)) suggest that they are mainly influenced by phytoplankton chlorophyll. Large variations in chlorophyll concentrations (0.2 to greater than 10 mg per cubic meters) correspond to variations in K(sub d) at 443 nm ranging from about 0.1 to greater than 1.5 per meter. Attenuation values at 443 nm generally peaked (or were minimal at 555 nm) at depths where chlorophyll concentrations were high. Above water remote sensing reflectance R(sub rs) (443) derived from E(sub d) and L(sub u) shows good agreement to surface chlorophyll. Ratios of remote sensing reflectance, R(sub rs)(443/R(sub rs)(555)versus chlorophyll suggests a potential for obtaining a suitable bio-optical algorithm for the region influenced by the Mississippi River.

D'Sa, E.↗

A Slow Retrieval Algorithm for Satellite and Surface Based Instruments

We present results of a retrieval algorithm for satellite and ground based instruments using the Arizona radiative transfer code. A state vector describing the atmospheric and surface condition is iteratively modified until the calculated radiances match the observed values. Elements of the state vector include: aerosol concentrations, radius, optical properties, mass-weighted altitudes, chlorophyll concentration and wind speed. While computationally expensive, many assumptions used in other retrieval algorithms are not invoked. We present co-located retrievals for MODIS, SEAWIFS and nearby AERONET sites. MODIS AQUA and SEA WIFS: Ten MODIS (.412 - 2.110 microns) and eight SEA WIFS (.412-.865 microns) radiances (.412-.865 microns) include channels where aerosols absorb and reflect radiation. We focus on retrieving bio-mass burning aerosols that are advected over open ocean. Since chlorophyll absorbs at frequencies where black carbon absorbs, our retrieval algorithm accounts for chlorophyll absorption by simultaneously retrieving both aerosol and chlorophyll amount. Our retrieved chlorophyll concentrations are similar to those from the Ocean Color Group. AERONET: Both Almucantar and Principle plane radiances are used to retrieve the state of the atmosphere and ocean conditions. Our retrieved aerosol size distributions and optical properties are consistent with the aerosol inversions from the AERONET group.

Weaver, C.↗

The Ocean Colour Climate Change Initiative: III. A Round-Robin Comparison on In-Water Bio-Optical Algorithms

Satellite-derived remote-sensing reflectance (Rrs) can be used for mapping biogeochemically relevant variables, such as the chlorophyll concentration and the Inherent Optical Properties (IOPs) of the water, at global scale for use in climate-change studies. Prior to generating such products, suitable algorithms have to be selected that are appropriate for the purpose. Algorithm selection needs to account for both qualitative and quantitative requirements. In this paper we develop an objective methodology designed to rank the quantitative performance of a suite of bio-optical models. The objective classification is applied using the NASA bio-Optical Marine Algorithm Dataset (NOMAD). Using in situ Rrs as input to the models, the performance of eleven semianalytical models, as well as five empirical chlorophyll algorithms and an empirical diffuse attenuation coefficient algorithm, is ranked for spectrally-resolved IOPs, chlorophyll concentration and the diffuse attenuation coefficient at 489 nm. The sensitivity of the objective classification and the uncertainty in the ranking are tested using a Monte-Carlo approach (bootstrapping). Results indicate that the performance of the semi-analytical models varies depending on the product and wavelength of interest. For chlorophyll retrieval, empirical algorithms perform better than semi-analytical models, in general. The performance of these empirical models reflects either their immunity to scale errors or instrument noise in Rrs data, or simply that the data used for model parameterisation were not independent of NOMAD. Nonetheless, uncertainty in the classification suggests that the performance of some semi-analytical algorithms at retrieving chlorophyll is comparable with the empirical algorithms. For phytoplankton absorption at 443 nm, some semi-analytical models also perform with similar accuracy to an empirical model. We discuss the potential biases, limitations and uncertainty in the approach, as well as additional qualitative considerations for algorithm selection for climate-change studies. Our classification has the potential to be routinely implemented, such that the performance of emerging algorithms can be compared with existing algorithms as they become available. In the long-term, such an approach will further aid algorithm development for ocean-colour studies.

Phytoplankton↗

A comparison of global estimates of marine primary production from ocean color

The third primary production algorithm round robin (PPARR3) compares output from 24 models that estimate depth-integrated primary production from satellite measurements of ocean color, as well as seven general circulation models (GCMs) coupled with ecosystem or biogeochemical models. Here we compare the global primary production fields corresponding to eight months of 1998 and 1999 as estimated from common input fields of photosynthetically-available radiation (PAR), sea-surface temperature (SST), mixed-layer depth, and chlorophyll concentration. We also quantify the sensitivity of the ocean-color-based models to perturbations in their input variables. The pair-wise correlation between ocean-color models was used to cluster them into groups or related output, which reflect the regions and environmental conditions under which they respond differently. The groups do not follow model complexity with regards to wavelength or depth dependence, though they are related to the manner in which temperature is used to parameterize photosynthesis. Global average PP varies by a factor of two between models. The models diverged the most for the Southern Ocean, SST under 10 C, and chlorophyll concentration exceeding 1mg Chlm-3. Based on the conditions under which the model results diverge most, we conclude that current ocean-color-based models are challenged by high-nutrient low-chlorophyll conditions, and extreme temperatures or chlorophyll concentrations. The GCM-based models predict comparable primary production to those based on ocean color: they estimate higher values in the Southern Ocean, at low SST, and in the equatorial band, while they estimate lower values in eutrophic regions (probably because the area of high chlorophyll concentrations is smaller in the GCMs). Further progress in primary production modeling requires improved understanding of the effect of temperature on photosynthesis and better parameterization of the maximum photosynthetic rate.

Yamanaka, Yasuhiro↗

Scaling photosynthetic function and CO2 dynamics from leaf to canopy level for maize – dataset combining diurnal and seasonal measurements of vegetation fluorescence, reflectance and vegetation indices with canopy gross ecosystem productivity

Recent advances in leaf fluorescence measurements and canopy proximal remote sensing currently enable the non-destructive collection of rich diurnal and seasonal time series, which are required for monitoring vegetation function at the temporal and spatial scales relevant to the natural dynamics of photosynthesis. Remote sensing assessments of vegetation function have traditionally used actively excited foliar chlorophyll fluorescence measurements, canopy optical reflectance data and vegetation indices (VIs), and only recently passive solar induced chlorophyll fluorescence (SIF) measurements. In general, reflectance data are more sensitive to the seasonal variations in canopy chlorophyll content and foliar biomass, while fluorescence observations more closely relate to the dynamic changes in plant photosynthetic function. With this dataset we link leaf level actively excited chlorophyll fluorescence, canopy proximal reflectance and SIF, with eddy covariance measurements of gross ecosystem productivity (GEP). The dataset was collected during the 2017 growing season on maize, using three automated systems (i.e., Monitoring Pulse-Amplitude-Modulation fluorimeter, Moni-PAM; Fluorescence Box, FloX; and from eddy covariance tower). The data were quality checked, filtered and collated to a common 30 minutes timestep. We derived vegetation indices related to canopy functioning (e.g., Photochemical Reflectance Index, PRI; Normalized Difference Vegetation Index, NDVI; Chlorophyll Red-edge, Clre) to investigate how SIF and VIs can be coupled for monitoring vegetation photosynthesis. The raw datasets and the filtered and collated data are provided to enable new processing and analyses.

Petya Campbell↗

Hilo Bay Water Resources: Monitoring Water Quality in Hilo Bay, Hawaii to Support Future Community Planning

Designated as an impaired body of water by both state and federal water quality standards, Hilo Bay, Hawaiʻi is highly susceptible to brown water, a condition where the water becomes murky and is associated with excess levels of bacteria, contaminants, and nutrients. A breakwater in Hilo Bay, which was established to protect Hilo town from tsunamis, interferes with water circulation and prolongs the presence of brown water in the bay. The State of Hawaiʻi issues brown water advisories (BWAs) following flash flood warnings, sewage spills, and other events to indicate a public health concern for those who use Hilo Bay for recreation, cultural purposes, and fishing. Due to the elevated public health risk and ecosystem disturbance that brown water poses to Hilo Bay, we partnered with the Hawaiʻi County Office of Sustainability, Climate, Equity, and Resilience (OSCER) to examine the feasibility of using Earth observations (EO) to monitor water quality in the Hilo Bay region. We leveraged data from Sentinel-2 Multispectral Instrument (MSI), Landsat 8 Operational Land Imager (OLI), Landsat 9 OLI-2, and Aqua and Terra Moderate Resolution Imagine Spectroradiometer (MODIS) instruments to identify and assess spatial and temporal patterns of two main water quality parameters, turbidity and chlorophyll-a, during BWAs. We used the Optical Reef and Coastal Area Assessment (ORCAA) tool in Google Earth Engine to process EO data and generate water quality maps and time series. Our study found that increased turbidity levels can be identified by EO data during BWAs. In addition, our map products indicated the presence of several turbidity plumes along the coast, with the highest concentration of turbidity found within Hilo Bay. While chlorophyll-a levels were relatively flat within our study region during BWAs, we found that regional chlorophyll-a patterns could be derived from MODIS chlorophyll-a data in NASA Worldview. Our study’s multi-sensor approach provided valuable insights for how water quality in the Hilo Bay region can be monitored in the future.

remote sensing↗

Aerial photography for sensing plant anomalies

Changes in the red tonal response of Kodak Ektrachrome Infrared Aero 8443 film (EIR) are often incorrectly attributed solely to variations in infrared light reflectance of plant leaves, when the primary influence is a difference in visible light reflectance induced by varying chlorophyll contents. Comparisons are made among aerial photographic images of high- and low-chlorophyll foliage. New growth, foot rot, and boron and chloride nutrient toxicites produce low-chlorophyll foliage, and EIR transparency images of light red or white compared with dark-red images of high-chlorophyll foliage. Deposits of the sooty mold fungus that subsists on the honeydew produced by brown soft scale insects, obscure the citrus leaves' green color. Infected trees appear as black images on EIR film transparencies compared with red images of healthy trees.

Gausman, H. W.↗

Remote sensing of phytoplankton density and diversity in Narragansett Bay using an airborne fluorosensor

An aircraft-borne remote system is presented that utilizes narrow-band light from multiple dye lasers to excite selected algae photopigments and then measures the resultant flourescence emitted from chlorophyll a at 685 nm. Tests were conducted with both pure and mixed cultures of marine algae from a series of field tests taken from piers and bridges of Narragansett Bay, and a prototype remote fluorosensor was flown over the Bay during the 1978 winter-spring diatom bloom. Remote fluorescence obtained at hover points over sea-truth stations showed correlations with in situ fluorescence, total chlorophyll a, and cell count. It was concluded that the ratio of remote fluorescence to direct chlorophyll a concentration was less variable than expected, and the distribution of total chlorophyll a between two major photoplankton color groups showed three distinct areas, within the Bay, of green and golden-brown species.

Farmer, F. H.↗

Laboratory tank studies of a single species of phytoplankton using a remote sensing fluorosensor

Phytoplankton were grown in the laboratory for the purpose of testing a remote fluorosensor. The fluorosensor uses a unique four-wavelength dye laser system to excite phytoplankton bearing chlorophyll and to measure the chlorophyll fluorescence generated by this excitation. Six different species were tested, one at a time, and each was grown two to four times. Fluorescence measured by the fluorosensor provides good quantitative measurement of chlorophyll concentrations for all species tested while the cultures were in log phase growth. Fluorescene cross section ratios obtained in the single species tank tests support the hypothesis that the shape of the fluorescence cross section curve remains constant with the species (differences in fluorescence cross section ratios are a basis for determining composition of phytoplankton according to color group when a multiwavelength source of excitation is used. Linear relationships exist between extracted chlorophyll concentration and fluorescence measured by the remote fluorosensor during the log phase growth of phytoplankton cultures tested.

Brown, C. A., Jr.↗

Analysis of testbed airborne multispectral scanner data from Superflux II

A test bed aircraft multispectral scanner (TBAMS) was flown during the James Shelf, Plume Scan, and Chesapeake Bay missions as part of the Superflux 2 experiment. Excellent correlations were obtained between water sample measurements of chlorophyll and sediment and TBAMS radiance data. The three-band algorithms used were insensitive to aircraft altitude and varying atmospheric conditions. This was particularly fortunate due to the hazy conditions during most of the experiments. A contour map of sediment, and also chlorophyll, was derived for the Chesapeake Bay plume along the southern Virginia-Carolina coastline. A sediment maximum occurs about 5 nautical miles off the Virginia Beach coast with a chlorophyll maximum slightly shoreward of this. During the James Shelf mission, a thermal anomaly (or front) was encountered about 50 miles from the coast. There was a minor variation in chlorophyll and sediment across the boundary. During the Chesapeake Bay mission, the Sun elevation increased from 50 degrees to over 70 degrees, interfering with the generation of data products.

Bowker, D. E.↗

Analysis of ocean color scanner data from the Superflux III Experiment

The scanner collected data on October 15, 20, and 22, 1980. Single-channel gray-scale data products generated 5 minutes after the scanner were collected and showed details of the Chesapeake plume structure, suggesting that this quick-look capability could have potential use for experiments in real time. The Chesapeake Bay plume extended offshore about 5 nautical miles on October 15, and 7 nautical miles on October 20. Using the October 15 1980, data, a correlation coefficient of r = 0.889 was obtained between chlorophyll 'A' and the ratio of band 7 (664-684 nanometers) to band 9 (746-766 nanometers). This ratio was then used to calculate the average surface chlorophyll 'A' concentration of the water flowing out of the Chesapeak Bay. A ratio from the Ocean Color Scanner bands was created to simulate the ratio that the Multichannel Ocean Color Sensor uses to calculate chlorophyll A concentrations. Using the October 15, 1980, data set, this ratio has a correlation coefficient of r = -0.739 with the log of the chlorophyll 'A' and the ratio of band 2 minus band 4 to band 2 plus band 4 of the Ocean Color Scanner. No correlation is found between the Ocean Color Scanner data and total suspended solid measurements made on October 15, 1980.

Ohlhorst, C. W.↗

Detection of hidden mineral deposits by airborne spectral analysis of forest canopies

Data from field surveys and biogeochemical tests conducted in Maine, Montana, and Washington strongly correlate with results obtained using high resolution airborne spectroradiometer which detects an anomalous spectral waveform that appears definitely associated with sulfide mineralization. The spectral region most affected by mineral stress is between 550 nm and 750 nm. Spectral variations observed in the field occur on the wings of the red chlorophyll band centered at about 690 nm. The metal-stress-induced variations on the absorption band wing are most successfully resolved in the high spectral resolution field data using a waveform analysis technique. The development of chlorophyll pigments was retarded in greenhouse plants doped with copper and zinc in the laboratory. The lowered chlorophyll production resulted in changes on the wings of the chlorophyll bands of reflectance spectra of the plants. The airborne spectroradiometer system and waveform analysis remains the most sensitive technique for biogeochemical surveys.

Collins, W.↗

Coastal Zone Color Scanner data of rich coastal waters

Comparisons of chlorophyll concentrations and diffuse attenuation coefficients measured from ships off the central California coast were made with satellite derived estimates of the same parameters using data from the Coastal Zone Color Scanner. Very high chlorophyll concentrations were encountered in Monterey Bay. Although lower chlorophyll values acquired off Pt. Sur agreed satisfactorily with the satellite data, the high chlorophyll values departed markedly from agreement. Two possible causes for the disagreement are suggested. Comparison of diffuse attenuation coefficients from the same data sets showed closer agreement.

Wrigley, R. C.↗

Active-passive airborne ocean color measurement. II - Applications

Reported here for the first time is the use of a single airborne instrument to make concurrent measurements of oceanic chlorophyll concentration by (1) laser-induced fluorescence, (2) passive upwelling radiance, and (3) solar-induced chlorophyll fluorescence. Results from field experiments conducted with the NASA airborne oceanographic lidar (AOL) in the New York Bight demonstrate the capability of a single active-passive instrument to perform new and potentially important ocean color studies related to (1) active lidar validation of passive ocean color in-water algorithms, (2) chlorophyll a in vivo fluorescence yield variability, (3) calibration of active multichannel lidar systems, (4) effect of sea state on passive and active ocean color measurements, (5) laser/solar-induced chlorophyll fluorescence investigations, and (6) subsequent improvement of satellite-borne ocean color scanners. For validation and comparison purposes a separate passive ocean color sensor was also flown along with the new active-passive sensor during these initial field trials.

Hoge, F. E.↗

Coupling of Coastal Zone Color Scanner data to a physical-biological model of the southeastern U.S. continental shelf ecosystem. I - CZCS data description and Lagrangian particle tracing experiments. II - An Eulerian model. III - Nutrient and phytoplankton fluxes and CZCS data assimilation

Surface phytoplankton biomass of the southeastern U.S. continental shelf area is discussed based on coastal zone color scanner (CZCS) images obtained in April 1980. Data of chlorophyll distributions are analyzed in conjunction with concurrent flow and temperature fields. Lagrangian particle tracing experiments show that the particles move consistently with the evolution of the chlorophyll patterns. A four-component physical-biological model for a horizontal plane at a nominal depth of 17 m is presented. Model simulations using various physical-biological dynamics and boundary conditions show that the variability of chlorophyll distributions is controlled by horizontal advection. Phytoplankton and nutrient fluxes, calculated using the model, show considerable variability with time. The chlorophyll distributions obtained from the CZCS images are assimilated into the model to improve the phytoplankton flux estimates.

Ishizaka, Joji↗