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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 271 records · Page 15
All sourcesNASA NTRSDOE OSTI
DOE OSTI2023

Using light and X-ray scattering to untangle complex neuronal orientations and validate diffusion MRI

Disentangling human brain connectivity requires an accurate description of nerve fiber trajectories, unveiled via detailed mapping of axonal orientations. However, this is challenging because axons can cross one another on a micrometer scale. Diffusion magnetic resonance imaging (dMRI) can be used to infer axonal connectivity because it is sensitive to axonal alignment, but it has limited spatial resolution and specificity. Scattered light imaging (SLI) and small-angle X-ray scattering (SAXS) reveal axonal orientations with microscopic resolution and high specificity, respectively. Here, we apply both scattering techniques on the same samples and cross-validate them, laying the groundwork for ground-truth axonal orientation imaging and validating dMRI. We evaluate brain regions that include unidirectional and crossing fibers in human and vervet monkey brain sections. SLI and SAXS quantitatively agree regarding in-plane fiber orientations including crossings, while dMRI agrees in the majority of voxels with small discrepancies. We further use SAXS and dMRI to confirm theoretical predictions regarding SLI determination of through-plane fiber orientations. Scattered light and X-ray imaging can provide quantitative micrometer 3D fiber orientations with high resolution and specificity, facilitating detailed investigations of complex fiber architecture in the animal and human brain.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

Retinal microvascular and neuronal pathologies probed in vivo by adaptive optical two-photon fluorescence microscopy

The retina, behind the transparent optics of the eye, is the only neural tissue whose physiology and pathology can be non-invasively probed by optical microscopy. The aberrations intrinsic to the mouse eye, however, prevent high-resolution investigation of retinal structure and function in vivo. Optimizing the design of a two-photon fluorescence microscope (2PFM) and sample preparation procedure, we found that adaptive optics (AO), by measuring and correcting ocular aberrations, is essential for resolving putative synaptic structures and achieving three-dimensional cellular resolution in the mouse retina in vivo. Applying AO-2PFM to longitudinal retinal imaging in transgenic models of retinal pathology, we characterized microvascular lesions with sub-capillary details in a proliferative vascular retinopathy model, and found Lidocaine to effectively suppress retinal ganglion cell hyperactivity in a retinal degeneration model. Tracking structural and functional changes at high-resolution longitudinally, AO-2PFM enables microscopic investigations of retinal pathology and pharmacology for disease diagnosis and treatment in vivo.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1980

Study on the neuronal circuits implicated in postural tremor and hypokinesia

The effect of various tegmentary lesions at the level of the pontomesenchphalon in monkeys on motor function was observed. The importance of the monoaminergic mechanisms of the brainstem is discussed. The results also show the importance of the descending tegmentary rubral system and the rubroolivocerebellar circuit in controlling peripheral motor activity. The destruction of the sensory motor cortex proves to be a more effective way of eliminating spontaneous or harmaline induced tremor than the complete interruption of the pyramidal system on the level of the cerebral peduncle.

Poirier, L. J.↗
NASA NTRS1980

Neuron analysis of visual perception

The receptive fields of single cells in the visual system of cat and squirrel monkey were studied investigating the vestibular input affecting the cells, and the cell's responses during visual discrimination learning process. The receptive field characteristics of the rabbit visual system, its normal development, its abnormal development following visual deprivation, and on the structural and functional re-organization of the visual system following neo-natal and prenatal surgery were also studied. The results of each individual part of each investigation are detailed.

Chow, K. L.↗
NASA NTRS1980

Morphological changes in neurons of the hind limb reflex arc during long term immobilization

Twelve adult rabbits were immobilized for 9 to 31 days, followed by histological study of the nerve processes of lumbar vertebra 7 and sacral vertebra 1, the sciatic nerve and the motor endings of the thigh muscles. In the spinal ganglia, dystrophic changes of increasing severity with immobilization time were found, including pericellular edema, vacuolized neuroplasm, pycnotic changes, cytolysis and destruction. Chromatophilic matter decreased and was partly bleached, and amitotic division occurred. A portion of the sciatic nerve fibers were argentophilic, and some fragmentary decomposition occurred. Considerable dystrophic changes occurred in the motor nerve endings.

Tkachenko, Z. Y.↗
NASA NTRS1984

Rodent CNS neuron development: Timing of cell birth and death

Data obtained from a staged series of single paired injections of tritiated thymidine to pregnant Wistar rats or C57B16/j mice on selected embryonic days and several postnatal times are reported. All injected specimens were allowed to come to term, each litter culled to six pups and specimens were sacrificed on PN28, with fixation and embedding for paraffin and plastic embedding. The results are derived from serial paraffin sections of PN28 animals exposed to autoradiographic processing and plotted with respect to heavily labelled cell nuclei present in the selected brain stem nuclei and sensory ganglia. Counts from each time sample/structure are totalled and the percentage of cells in the total labelled population/structure represented by each injection time interval plotted.

Keefe, J. R.↗
NASA NTRS1995

Microgravity and Charge Transfer in the Neuronal Membrane: Implications for Computational Neurobiology

Evidence from natural and artificial membranes indicates that the neural membrane is a liquid crystal. A liquid-to-gel phase transition caused by the application of superposed electromagnetic fields to the outer membrane surface releases spin-correlated electron pairs which propagate through a charge transfer complex. The propagation generates Rydberg atoms in the lipid bilayer lattice. In the present model, charge density configurations in promoted orbitals interact as cellular automata and perform computations in Hilbert space. Due to the small binding energies of promoted orbitals, their automata are highly sensitive to microgravitational perturbations. It is proposed that spacetime is classical on the Rydberg scale, but formed of contiguous moving segments, each of which displays topological equivalence. This stochasticity is reflected in randomized Riemannian tensor values. Spacetime segments interact with charge automata as components of a computational process. At the termination of the algorithm, an orbital of high probability density is embedded in a more stabilized microscopic spacetime. This state permits the opening of an ion channel and the conversion of a quantum algorithm into a macroscopic frequency code.

Wallace, Ron↗
NASA NTRS2001

Simultaneous Spectral and Timing Observations of Accreting Neuron Stars

The goal of this proposal was to perform simultaneous x-ray spectral and millisecond timing observations of accreting neutron stars to further our understanding of their accretion dynamics and in the hope of using these systems as probes of the physics of strong gravitational fields. Observations of the neutron star binaries 4U0614+091, 4U1728-34, 4U1820-30, and Cyg X-2 were carried out with RXTE and BeppoSAX, ASCA, and Chandra (not all simultaneously). In addition, archival data were analyzed for 4U0614+091 and 4U1820-30. This investigation led to publication of three papers in peer-reviewed journals. These are listed below. In addition, the results were presented at several meetings including the two poster presentations listed below. Dr. Santina Piraino visited SAO for 4 months during 2000 to collaborate on analysis of the data from NAG5-8408 and NAG5-9104.

Kaaret, P.↗
NASA NTRS2002

Simultaneous Spectral and Timing Observations of Accreting Neuron Stars

The goal of this proposal is to perform simultaneous x-ray spectral and millisecond timing observations of accreting neutron stars to further our understanding of their accretion dynamics and in the hope of using these systems as probes of the physics of strong gravitational fields. NAG5-9104 is the successor grant to NAG5-8408. Observations using the Rossi X-Ray Timing Explorer (RXTE) and BeppoSAX were performed of 4U1702-429, 4U1735-44, and Cyg X-2. Unfortunately, only a small fraction of the approved observing time was obtained for the first two targets and the data are of limited scientific value. Data analysis has been completed on the observations of Cyg X-2. We discovered a correlation between the frequency of the horizontal branch oscillations (HBO) and a soft, thermal component of the x-ray spectrum likely associated with emission from the accretion disk. This correlation may place constraints on models of the oscillations. A paper based on these results appeared in the Astrophysical Journal.

Kaaret, P.↗
NASA NTRS2003

Morphological properties of vestibulospinal neurons in primates

The lateral and medial vestibulospinal tracts constitute the major descending pathways controlling extensor musculature of the body. We examined the axon morphology and synaptic input patterns and targets in the cervical spinal segments from these tract cells using intracellular recording and biocytin labeling in the squirrel monkey. Lumbosacral projecting cells represent a private, and mostly rapid, communication pathway between the dorsal Deiters' nucleus and the motor circuits controlling the lower limbs and tail. The cervical projecting cells provide both redundant and variable synaptic input to spinal cell groups, suggesting both general and specific control of the head and neck reflexes.

Non-programmatic↗