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NASA NTRS1992

Fluorescence of thermal control coatings on S0069 and A0114

Many of the thermal control surfaces exposed to the space environment during the 5.8 year LDEF mission experienced changes in fluorescence. All of the thermal control coatings flown on LDEF experiments S0069 and A0114 were characterized for fluorescence under ambient conditions. Some of the black coatings, having protective overcoats, appear bright yellow under ultraviolet exposure. Urethane based coatings exhibited emission spectra shifts toward longer wavelengths in the visible range. Zinc oxide pigment based coatings experienced a quenching of fluorescence, while zinc orthotitanate pigment based and other ceramic type coatings had no measurable fluorescence.

Zwiener, James M.↗
NASA NTRS1992

Effect of DCB extraction on Mossbauer and spectral data for a Hawaiian palagonitic soil (PN-9): Iden tification of pigmentary phases

The study of palagonitic soils is an active area of research in martian geoscience because the spectral and magnetic properties of a subset are spectral and/or magnetic analogues of martian bright regions. An understanding of the composition, distribution, and mineralogy of ferric-bearing phases for palagonitic soils forms, through spectral and magnetic data, a basis for inferring the nature of ferric-bearing phases on Mars. Progress has been made in this area, but the data set is incomplete, especially with respect to the nature of pigmenting phases. The purpose of this study is to identify the nature of the pigment for Hawaiian palagonitic soil PN-9 by using extraction procedures to selectively remove iron oxide phases. This soil was collected at the same locale as samples Hawaii 34 and VOL02. All three soils are good spectral analogues for martian bright regions.

Morris, Richard V.↗
NASA NTRS1993

An assessment of the Nimbus-7/CZCS calibration for May 1986 using satellite and in situ data from the Arabian Sea

A hydrographic survey of the central Arabian Sea was conducted in May 1986 and stations were correlated with Nimbus-7/Coastal Zone Color Scanner (CZCS) overpasses. As calibration of the CZCS degraded severely during its lifetime, pigment concentrations from samples at four sites in the Arabian Sea were used to refine the calibration of the CZCS. This vicarious calibration technique provided a final estimate of the sensor's sensitivity loss for sensor Gain 2. Calibration using normalized water-leaving radiance for the 520 nm and 550 nm bands showed considerable final sensor degradation. Future missions must incorporate calibration validation programs in order to maintain derived product accuracy requirements. Profiles of pigment concentration, salinity, temperature, and light attenuation are used to describe the bio-optical characteristics of the Arabian Sea during the intermonsoon period.

Hay, Bernward J.↗
NASA NTRS1992

Seasonal and spatial variations in phytoplanktonic chlorophyll in eutrophic Mono Lake, California, measured with the Airborne Visible and Infrared Imaging Spectrometer (AVIRIS)

The principal problem with application of airborne imaging spectrometers to lakes is the weak upwelling signal, especially when narrow spectral bands with high spatial resolution are sought. Furthermore, atmospheric path radiance dominates the signal received from dark targets such as lakes. Once atmospheric effects have been removed from the radiance received at the sensor, semi-empirical relationships can be developed to extract information about phytoplankton pigment concentrations for different underwater optical conditions. In lakes where concentrations of dissolved organics and suspended detritus may not co-vary with phytoplankton pigments, the many spectral channels of an imaging spectrometer such as AVIRIS are likely to be required to distinguish the various aquasols. The objectives of our study are to: (1) estimate the chlorophyll content of a lake with hundred-fold seasonal ranges in chlorophyll concentration using atmospherically corrected upwelling radiances derived from AVIRIS imagery, and (2) to examine spatial patterns in chlorophyll after reduction of the coherent noise in the imagery by filtering techniques.

Melack, John M.↗
NASA NTRS1992

Tracking photosynthetic efficiency with narrow-band spectroradiometry

Narrow-waveband spectroradiometry presents the possibility of detecting subtle signals closely related to the current physiological state of vegetation. One such signal related to the epoxidation state of the xanthophyll cycle pigments, violaxanthin, antheraxanthin, and zeaxanthin is discussed. Recent advances in plant ecophysiology demonstrated a close relationship between these pigments and the regulatory state of photosystem 2 in photosynthesis. Our recent field studies of sunflower (Helianthus annuus) and oak (Quercus agrifolia) demonstrated that a 'xanthophyll signal' can be isolated from the diurnal reflectance spectra of intact canopies. Furthermore, the xanthophyll signal can be used to derive a 'physiological reflectance index' (PRI) that closely correlates with the actual photosynthetic efficiency (defined as the photosynthetic rate divided by the incident PAR) in closed canopies. If these signals were detectable in Airborne Visible/Infrared Imaging Spectrometers (AVIRIS) images, they could lead to improved remote estimates of photosynthetic fluxes.

Gamon, John A.↗
NASA NTRS1994

Visible-infrared remote-sensing model and applications for ocean waters

Remote sensing has become important in the ocean sciences, especially for research involving large spatial scales. To estimate the in-water constituents through remote sensing, whether carried out by satellite or airplane, the signal emitted from beneath the sea surface, the so called water-leaving radiance (L(w)), is of prime importance. The magnitude of L(w) depends on two terms: one is the intensity of the solar input, and the other is the reflectance of the in-water constituents. The ratio of the water-leaving radiance to the downwelling irradiance (E(d)) above the sear surface (remote-sensing reflectance, R(sub rs)) is independent of the intensity of the irradiance input, and is largely a function of the optical properties of the in-water constituents. In this work, a model is developed to interpret r(sub rs) for ocean water in the visible-infrared range. In addition to terms for the radiance scattered from molecules and particles, the model includes terms that describe contributions from bottom reflectance, fluorescence of gelbstoff or colored dissolved organic matter (CDOM), and water Raman scattering. By using this model, the measured R(sub rs) of waters from the West Florida Shelf to the Mississippi River plume, which covered a (concentration of chlorophyll a) range of 0.07 - 50 mg/cu m, were well interpreted. The average percentage difference (a.p.d.) between the measured and modeled R(sub rs) is 3.4%, and, for the shallow waters, the model-required water depth is within 10% of the chart depth. Simple mathematical simulations for the phytoplankton pigment absorption coefficient (a(sub theta)) are suggested for using the R(sub rs) model. The inverse problem of R(sub rs), which is to analytically derive the in-water constituents from R(sub rs) data alone, can be solved using the a(sub theta) functions without prior knowledge of the in-water optical properties. More importantly, this method avoids problems associated with a need for knowledge of the shape and value of the chlorophyll-specific absorption coefficient. The simulation was tested for a wide range of water types, including waters from Monterey Bay, the West Florida Shelf, and the Mississippi River plume. Using the simulation, the R(sub rs)-derived in-water absorption coefficients were consistent with the values from in-water measurements (r(exp 2) greater than 0.94, slope approximately 1.0). In the remote-sensing applications, a new approach is suggested for the estimation of primary production based on remote sensing. Using this approach, the calculated primary production (PP) values based upon remotely sensed data were very close to the measured values for the euphotic zone (r(exp 2) = 0.95, slope 1.26, and 32% average difference), while traditional, pigment-based PP model provided values only one-third the size of the measured data. This indicates a potential to significantly improve the accuracy of the estimation of primary production based upon remote sensing.

Lee, Zhongping↗
NASA NTRS1993

The red edge in arid region vegetation: 340-1060 nm spectra

The remote sensing study of vegetated regions of the world has typically been focused on the use of broad-band vegetation indices such as NDVI. Various modifications of these indices have been developed in attempts to minimize the effect of soil background, e.g., SAVI, or to reduce the effect of the atmosphere, e.g., ARVI. Most of these indices depend on the so-called 'red edge,' the sharp transition between the strong absorption of chlorophyll pigment in visible wavelengths and the strong scattering in the near-infrared from the cellular structure of leaves. These broadband indices tend to become highly inaccurate as the green canopy cover becomes sparse. The advent of high spectral resolution remote sensing instrument such as the Airborne Visible and Infrared Imaging Spectrometer (AVIRIS) has allowed the detection of narrow spectral features in vegetation and there are reports of detection of the red edge even for pixels with very low levels of green vegetation cover by Vane et al. and Elvidge et al., and to characterize algal biomass in coastal areas. Spectral mixing approaches similar to those of Smith et al. can be extended into the high spectral resolution domain allowing for the analysis of more endmembers, and potentially, discrimination between material with narrow spectral differences. Vegetation in arid regions tends to be sparse, often with small leaves such as the creosote bush. Many types of arid region vegetation spend much of the year with their leaves in a senescent state, i.e., yellow, with lowered chlorophyll pigmentation. The sparseness of the leaves of many arid region plants has the dual effect of lowering the green leaf area which can be observed and of allowing more of the sub-shrub soil to be visible which further complicates the spectrum of a region covered with arid region vegetation. Elvidge examined the spectral characteristics of dry plant materials showing significant differences in the region of the red edge and the diagnostic ligno-cellulose absorptions at 2090 nm and 2300 nm. Ray et al. detected absorption at 2100 nm in AVIRIS spectra of an abandoned field known to be covered by a great deal of dead plant litter. In order to better study arid region vegetation remote sensing data, it is necessary to better characterize the reflectance spectra of in situ, living, arid region plants.

Ray, Terrill W.↗
NASA NTRS1994

Minimizing systematic errors from atmospheric multiple scattering and satellite viewing geometry in coastal zone color scanner level IIA imagery

Water-leaving radiances and phytoplankton pigment concentrations are calculated from coastal zone color scanner (CZCS) radiance measurements by removing atmospheric Rayleigh and aerosol radiances from the total radiance signal measured at the satellite. The single greatest source of error in CZCS atmospheric correction algorithms in the assumption that these Rayleigh and aerosol radiances are separable. Multiple-scattering interactions between Rayleigh and aerosol components cause systematic errors in calculated aerosol radiances, and the magnitude of these errors is dependent on aerosol type and optical depth and on satellite viewing geometry. A technique was developed which extends the results of previous radiative transfer modeling by Gordon and Castano to predict the magnitude of these systematic errors for simulated CZCS orbital passes in which the ocean is viewed through a modeled, physically realistic atmosphere. The simulated image mathematically duplicates the exact satellite, Sun, and pixel locations of an actual CZCS image. Errors in the aerosol radiance at 443 nm are calculated for a range of aerosol optical depths. When pixels in the simulated image exceed an error threshhold, the corresponding pixels in the actual CZCS image are flagged and excluded from further analysis or from use in image compositing or compilation of pigment concentration databases. Studies based on time series analyses or compositing of CZCS imagery which do not address Rayleigh-aerosol multiple scattering should be interpreted cautiously, since the fundamental assumption used in their atmospheric correction algorithm is flawed.

Martin, D. L.↗
NASA NTRS1994

Ship and satellite observations of chlorophyll stocks in interacting cyclone-anticyclone eddy pairs in the western Gulf of Mexico

When anticyclonic eddies shed by the Loop Current of the Gulf of Mexico reach the western margin of the gulf, they influence the surface circulation over the continental slope and rise. Of particular interest is the generation of cyclone (cold-core)-anticyclone (warm-core) pairs when aging Loop Current eddies interact with the continental margin. In this paper we describe the physical and biological characteristics of these cyclone-anticyclone pairs. Our objective was to determine how eddy pairs affect the distribution of phytoplankton in the region and how satellite ocean color measurements are applicable to tracing of the eddies. We present shipboard data collected between 1980 and 1982 on the hydrography, chlorophyll stocks, and nutrient concentrations of eddy pairs in the western Gulf of Mexico and compare these data with coastal zone color scanner (CZCS) images collected during the time frame of the cruises. Surface pigment concentrations followed a seasonal cycle, with low concentrations (0.05-0.1 mg m(exp -3)) found within cyclones and anticyclones from April through early November and higher concentrations (greater than 0.1 mg(exp -3)) found in the winter. CZCS pigment concentrations were locally high in the flow confluence of cyclone-anticyclone pairs. The CZCS imagery shows that some cyclone-anticyclone geometries transport high-chlorophyll shelf water seaward at least 100-200 km off-shelf.

Biggs, Douglas, C.↗
NASA NTRS1995

Light response of phytoplankton in the South Atlantic Ocean: Interpretation of observations and application to remote sensing

A simplified, nonspectral derivation of a classical theory in plant physiology is presented and used to derive an absorption-based primary productivity algorithm. Field observations from a meridional transect (4 deg N to 42 deg S) in the Atlantic Ocean are then described and interpreted in this theoretical context. The observations include photosynthesis-irradiance curve parameters (alpha and P(sub max)), chlorophyll a and phaeopigment concentration, and estimated phytoplankton absorption coefficients at wavelength = 440 nm (a(sub ph)(440)). Observations near the top (50% I(sub 0)) and bottom (6% I(sub 0)) of the euphotic zone are contrasted. At both light levels, alpha, P(sub max), a(sub ph)(440), and pigment concentration varied similarly along the transect: values were highest at the equator and at the southern end of the transect and lowest in the central South Atlantic. It is concluded that this pattern was related to increased nutrient availability due to equatorial upwelling in the north, and increased wind mixing in the south. At the 50% light level, alpha increased relative to a(sub ph) at the southern end of the transect. This result appears to reflect a large-scale meridional (southward) increase in the average quantum efficiency of the photosynthetic units of the phytoplankton. A correlation analysis of the data reveals that at the 50% light level, variations in P(sub max) were more closely related to a(sub ph)(440) than chlorophyll concentration and that phytoplankton absorption explains 90% of the variability in P(sub max). In theory, this shows that the ratio of the average quantum efficiency of the photosynthetic units of the phytoplankton to the product of their average absorption cross section and turnover time is relatively constant. This result is used to simplify the absorption-based primary productivity algorithm derived previously. The feasibility of using this model to estimate production rate from satellite ocean color observations is discussed. It is concluded that an absorption-based algorithm should provide more accurate production rate estimates than one based upon chlorophyll (pigment) concentration.

Hood, Raleigh R.↗
NASA NTRS1994

UV-A/Blue-Light responses in algae

All life on earth depends on light. A variety of photoreceptors capture the light for a wide range of reactions. Photosynthetic organisms absorb the light necessary for energy transformation and charge separation facilitating photosynthesis. In addition to the bulk pigments there is a great diversity of photoreceptors present in minute concentrations that control development, metabolism and orientation of plants and microorganisms. Based on its spectral absorbance, the well-studied phytochrome system acts in the RL (red light) region as well as in the UV-A/BL (blue light) region where the above mentioned reactions are mediated by a variety of photoreceptors whose natures are largely unknown. Phyllogenetically the UV-A/BL photoreceptors seem to be more ancient pigments that eventually were replaced by the phytochrome system. However, there are many reports that suggest a coaction between the UV-A/BL receptors and the phytochrome system. In several cases the UV-A/BL activation is the prerequisite for the phytochrome reaction. Historically it was the German botanist Julius Sachs who first discovered in 1864 that phototropism in plants was due to BL reactions. It took over 70 years until Bunning (1937) and Galston and Baker (1949) rediscovered the BL response. Since then, an ever-increasing attention has been paid to this effect. In this contribution, the general aspect of UV-A/BL responses and especially the responsiveness of algae will be covered.

Senger, Horst↗
NASA NTRS1997

Bio-Optical Properties of the Arabian Sea as Determined by In Situ and Sea WiFS Data

The overall objective of this work was to characterize optical and fluorescence properties in the euphotic zone during two British Ocean Flux Study (BOFS) Arabian Sea cruises. This was later expanded in 1995 to include three U.S. JGOFS Arabian Sea Cruises. The region was to be divided into one or more "bio-optical provinces," within each of which a single set of regression models was to be developed to relate the vertical distribution of irradiance attenuation and normalized fluorescence (SF and NF) to remote sensing reflectance and diffuse attenuation coefficient. The working hypothesis was that over relatively large spatial and temporal scales, the vertical profiles of bio-optical properties were predictable. The specific technical objectives were: (1) To characterize the vertical distribution of the inherent and apparent optical properties by measuring downwelling and upwelling irradiances, upwelling radiances, scalar irradiance of PAR, and beam transmissions at each station - from these data, spectral diffuse attenuation coefficients, irradiance reflectances, remote sensing reflectances, surface-leaving radiances and beam attenuation coefficients were determined; (2) To characterize the spectral absorption of total particulate, detrital, and dissolved organic material at each station from discrete water samples; (3) To describe the vertical distribution of photoadaptive properties in the water column by measuring profiles of stimulated (SF) and natural (NF) fluorescence and examining relationships between SF and NF as a function of diffuse optical depth, pigment biomass and primary productivity; and (4) To establish locally derived, in-water algorithms relating remote sensing reflectance spectra to diffuse attenuation coefficients, phytoplankton pigment concentrations and primary productivity, through intercomparisons with in situ measurements, for application to SeaWiFS data.

Trees, Charles C.↗
NASA NTRS1997

Predicting Photosynthetic Fluxes from Spectral Reflectance of Leaves and Canopies

The central hypothesis of this study has been that photosynthetic efficiency and capacity can be predicted from 'physiological reflectance indices' derived from spectral reflectance of leaves and canopies. I have approached this topic with a combination of laboratory and field experiments, and have also explored the potential of deriving a meaningful physiological index from imaging spectrometry (e.g. AVIRIS). A few highlights are presented below. The main emphasis has been on the 'Photochemical Reflectance Index' (PRI), derived from reflectance at 531 nm and 570 nm. Unlike most 'conventional' vegetation indices (e.g. NDVI), PRI changes rapidly both with illumination and physiological state, because it detects the interconversion of xanthophyll cycle pigments, which serve as photoregulatory pigments and control energy distribution for the photosynthetic system. This approach has differed dramatically from most remote sensing in that it has emphasized temporal variation in narrow-band spectral signatures, instead of spatial patterns of broadband indices. Our primary conclusion has been that PRI works well as an index of photosynthetic light-use efficiency at the leaf scale, much in the same way as the fluorescence index DeltaF/Fm. However, unlike DeltaF/Fm which must be measured at close scales, PRI can be sampled at a range of spatial scales, presenting the possibility of monitoring photosynthetic fluxes remotely.

Gamon, John A.↗
NASA NTRS1999

Recovery of a Charred Painting Using Atomic Oxygen Treatment

A noncontact method is described which uses atomic oxygen to remove soot and char from the surface of a painting. The atomic oxygen was generated by the dissociation of oxygen in low pressure air using radio frequency energy. The treatment, which is an oxidation process, allows control of the amount of material to be removed. The effectiveness of char removal from half of a fire-damaged oil painting was studied using reflected light measurements from selected areas of the painting and by visual and photographic observation. The atomic oxygen was able to effectively remove char and soot from the treated half of the painting. The remaining loosely bound pigment was lightly sprayed with a mist to replace the binder and then varnish was reapplied. Caution should he used when treating an untested paint medium using atomic oxygen. A representative edge or corner should he tested first in order to determine if the process would be safe for the pigments present. As more testing occurs, a greater knowledge base will be developed as to what types of paints and varnishes can or cannot be treated using this technique. With the proper precautions, atomic oxygen treatment does appear to be a technique with great potential for allowing very charred, previously unrestorable art to be salvaged.

Rutledge, Sharon K.↗
NASA NTRS1999

Spectral Behavior of Hematite at Visible/Near Infrared and Midinfrared Wavelengths

The oxide mineral hematite alpha-Fe2O3) is present on Mars as evidenced by spectroscopy using visible/near infrared (VNIR) and midinfrared (MIR) wavelengths. The physical nature of this surficial hematite varies from fine-grained, crystalline red hematite (red-Hm) in certain martian bright regions, to coarser-grained, crystalline gray hematite (gray-Hm) in the Sinus Meridiani equatorial site discovered in the Mars Global Surveyor Thermal Emission Spectrometer (MGS-TES) data. The VNIR spectra require that the red hematite is present in amounts subordinate to a second ferric pigment, which accounts for the general shape and position of the martian ferric absorption edge. By analogy with palagonitic tephra and synthetic samples, the pigment is a nanophase ferric oxide, possibly nanophase hematite (np-Hm). The purpose of this abstract is to document the physicospectral properties of hematite in the VNIR (0.35 to 2.2 microns) and MIR (5 to 25 microns) with respect to the terms "nanophase" and "crystalline", "red" and "gray", and "fine-grained" and "coarse-grained". We will show that different "types" of hematite (no-, red-, and gray-Hm) have different spectral responses in the VNIR and MIR regions and that these differences are important for inferring the types of hematite and their relative proportions from remotely sensed spectra of Mars. Because hematite is an alteration product that forms through many different geologic processes, determination of the type of hematite provides important clues about the style and diversity of martian weathering processes.

Lane, Melissa D.↗
NASA NTRS2000

Ocean Optics Protocols for Satellite Ocean Color Sensor Validation

The document stipulates protocols for measuring bio-optical and radiometric data for the Sensor Intercomparison and Merger for Biological and Interdisciplinary Oceanic Studies (SIMBIOS) Project activities and algorithm development. This document supersedes the earlier version (Mueller and Austin 1995) published as Volume 25 in the SeaWiFS Technical Report Series. This document marks a significant departure from, and improvement on, theformat and content of Mueller and Austin (1995). The authorship of the protocols has been greatly broadened to include experts specializing in some key areas. New chapters have been added to provide detailed and comprehensive protocols for stability monitoring of radiometers using portable sources, abovewater measurements of remote-sensing reflectance, spectral absorption measurements for discrete water samples, HPLC pigment analysis and fluorometric pigment analysis. Protocols were included in Mueller and Austin (1995) for each of these areas, but the new treatment makes significant advances in each topic area. There are also new chapters prescribing protocols for calibration of sun photometers and sky radiance sensors, sun photometer and sky radiance measurements and analysis, and data archival. These topic areas were barely mentioned in Mueller and Austin (1995).

Fargion, Giulietta S.↗
NASA NTRS1999

Spectral Behavior of Hematite at Visible/Near Infrared and Midinfrared Wavelengths

The oxide mineral hematite alpha-Fe2O3) is present on Mars as evidenced by spectroscopy using visible/near infrared (VNIR) and midinfrared (MIR) wavelengths. The physical nature of this surficial hematite varies from fine-grained, crystalline red hematite (red-Hm) in certain martian bright regions, to coarser-grained, crystalline gray hematite (gray-Hm) in the Sinus Meridiani equatorial site discovered in the Mars Global Surveyor Thermal Emission Spectrometer (MGS-TES) data. The VNIR spectra require that the red hematite is present in amounts subordinate to a second ferric pigment, which accounts for the general shape and position of the martian ferric absorption edge. By analogy with palagonitic tephra and synthetic samples, the pigment is a nanophase ferric oxide, possibly nanophase hematite (np-Hm). The purpose of this abstract is to document the physicospectral properties of hematite in the VNIR (0.35 to 2.2 mm) and MIR (5 to 25 mm) with respect to the terms "nanophase" and "crystalline", "red" and "gray", and "fine-grained" and "coarse-grained". We will show that different "types" of hematite (np-, red-, and gray-Hm) have different spectral responses in the VNIR and MIR regions and that these differences are important for inferring the types of hematite and their relative proportions from remotely sensed spectra of Mars. Because hematite is an alteration product that forms through many different geologic processes, determination of the type of hematite provides important clues about the style and diversity of martian weathering processes. Additional information is contained in the original extended abstract.

Lane, M. D.↗
NASA NTRS1994

Plant Responses to Increased UV-B Radiation: A Research Project

Ozone decrease implies more ultraviolet-B (UV-B) radiation reaching the surface of the Earth. Increased UV-B radiation triggers responses by living organisms. Despite the large potential impacts on vegetation, little is known about UV-B effects on terrestrial ecosystems. Long-term ecological studies are needed to quantify the effects of increased UV radiation on terrestrial ecosystems, asses the risks, and produce reliable data for prediction. Screening pigments are part of one of the protective mechanism in plants. Higher concentrations of screening pigments in leaves may be interpreted as a response to increased UV radiation. If the screening effect is not sufficient, important molecules will be disturbed by incoming radiation. Thus, genetics, photosynthesis, growth, plant and leaf shape and size, and pollen grains may be affected. This will have an impact on ecosystem dynamics, structure and productivity. It is necessary to monitor selected terrestrial ecosystems to permit detection and interpretation of changes attributable to global climate change and depleted ozone shield. The objectives of this project are: (1) To identify and measure indicators of the effects of increased solar UV-B radiation on terrestrial plants; (2) to select indicators with the greatest responses to UV-B exposure; (3) to test, adapt or create ecosystem models that use the information gathered by this project for prediction and to enhance our understanding of the effects of increased UV-B radiation on terrestrial ecosystems. As a first step to achieve these objectives we propose a three-year study of forest and steppe vegetation on the North slope of the Brooks Range (within the Arctic circle, in Alaska), in the Saguaro National Monument (near Tucson, Arizona) and in the forests and steppes of Patagonia (Argentina). We selected (1) vegetation north of the Polar Circle because at 70N there is 8% risk of plant damage due to increased UV-B radiation; (2) the foothills of Catalina Mountains because there is anecdotal evidence of plant damage on the saguaros that has been linked to increased UV radiation, and (3) the forests of Nothofagus spp. and the steppe of Patagonia where the risk of plant damage at 35S is 5% and increases to as much as 15% at 55S due to increased UV-B radiation. Measurements of UV-B radiation impinging on the surface at 55S largely exceed the predicted UV-B radiation values at 50 latitude and 0% ozone depletion. Preliminary HPLC analyses of UV-B absorbing compounds in Nothofagus antartica, N. pumilio, N. betuloides and Rumex sp. in natural conditions show species-specific patterns. The spectrum of N. antartica grown at 38S differs significantly from that of N. antartica in natural conditions in Ushuaia (55S). These results suggest that the selected main area (Patagonia) is appropriate for assessing the problem and its magnitude and that Nothofagus is appropriate for our study.

DAntoni, H. L.↗