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At least 271 records · Page 15

Thiol redox proteomics: Characterization of thiol-based post-translational modifications

Redox post-translational modifications on cysteine thiols (redox PTMs) have profound effects on protein structure and function, thus enabling regulation of various biological processes. Redox proteomics approaches aim to characterize the landscape of redox PTMs at the systems level. These approaches facilitate studies of condition-specific, dynamic processes implicating redox PTMs and have furthered our understanding of redox signaling and regulation. Mass spectrometry (MS) is a powerful tool for such analyses which has been demonstrated by significant advances in redox proteomics during the last decade. A group of well-established approaches involves the initial blocking of free thiols followed by selective reduction of oxidized PTMs and subsequent enrichment for downstream detection. Alternatively, novel chemoselective probe-based approaches have been developed for various redox PTMs. Direct detection of redox PTMs without any enrichment has also been demonstrated given the sensitivity of contemporary MS instruments. This review discusses the general principles behind different analytical strategies and covers recent advances in redox proteomics. Several applications of redox proteomics are also highlighted to illustrate how large-scale redox proteomics data can lead to novel biological insights.

59 BASIC BIOLOGICAL SCIENCES↗

Initial in-orbit operation of the soft X-ray spectrometer Resolve onboard the X-ray imaging and spectroscopy mission satellite

The X-Ray Imaging and Spectroscopy Mission satellite was launched on September 6, 2023 (UT). Its Resolve instrument is a high-resolution X-ray spectrometer enabled by a microcalorimeter array thermally anchored to a 50-mK heat sink. Many sensitive, critical sub-systems comprise Resolve, including a multistage cryogenic cooling system, thin-film aperture filters, low-noise electronics, on-board signal processing, and several sources of X-rays for calibration. We summarize the initial on-orbit power-on and checkout of Resolve that commenced immediately after launch. Soon after launch, the cryocoolers were activated, and their operation was successfully established. On October 9, 2023, the first cycle of the adiabatic demagnetization refrigerator was carried out, bringing the sensors to their steady-state operational temperatures. Following this, the energy resolution at 5.9 keV was successfully measured. The energy scale of the system is highly sensitive to the thermal environment surrounding both the sensors and their analog electronics. Gain correction was performed using reference X-ray lines from onboard calibration sources. To optimize cooler frequency settings, noise spectra were collected across a range of frequencies, and the most suitable frequency pair was selected based on the in-orbit environment. During the final phase of the checkout, an attempt was made to open the gate valve, which is designed to protect the Dewar’s interior from external pressure during ground operations and launch. Unfortunately, this attempt was unsuccessful. As a result, the checkout process was temporarily paused, and a stable operational strategy was subsequently developed to enable Resolve to function effectively with the gate valve remaining closed.

X-ray micro-calorimeter↗

TDD LoRa and Delta Encoding in Low-Power Networks of Environmental Sensor Arrays for Temperature and Deformation Monitoring: Supporting Data

This dataset was used to assess the performance of a compression algorithm for Soil Deformation and Temperature Monitoring data. The development of this technology is presented in the article named time division duplex (TDD) "TDD LoRa and Delta Encoding in Low-Power Networks of Environmental Sensor Arrays for Temperature and Deformation Monitoring" and published in Springer's Journal on Signal Processing Systems. [Time Division Duplex (TDD); Long Range (LoRa)]Field experiment data includes: 1) soil temperature measurements, 2) snow temperature data, 3) soil temperature and deformation data. In these files, the first column contains the time (UTC), followed by the sensor's battery voltage and temperature and acceleration values (X, Y, Z) in subsequent columns. The measurements were acquired every 15 minutes. Lists included for probe, datalogger, and packaging components. The two temperature data sets were acquired at a site at the East River Watershed, Colorado, while the temperature/deformation data were collected at the Teller Road (mile 27) site near Nome, Alaska. The package includes 8 comma-separated files (.csv) and one .pdf file.The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

Fiber-optic three axis magnetometer prototype development

The goal of this research program was to develop a high sensitivity, fiber optic, interferometric, three-axis magnetometer for interplanetary spacecraft applications. Dynamics Technology, Inc. (DTI) has successfully integrated a low noise, high bandwidth interferometer with high sensitivity metallic glass transducers. Also, DTI has developed sophisticated signal processing electronics and complete data acquisition, filtering, and display software. The sensor was packaged in a compact, low power and weight unit which facilitates deployment. The magnetic field sensor had subgamma sensitivity and a dynamic range of 10(exp 5) gamma in a 10 Hz bandwidth. Furthermore, the vector instrument exhibited the lowest noise level when only one axis was in operation. A system noise level of 1 gamma rms was observed in a 1 Hz bandwidth. However, with the other two channels operating, the noise level increased by about one order of magnitude. Higher system noise was attributed to cross-channel interference among the dither fields.

Wang, Thomas D.↗

Hydrological Variations in Australia Recovered by GRACE High-Resolution Mascons Solutions

Australia represents a challenging region in which to study hydrological variations as recovered by the GRACE (Gravity Recovery And Climate Experiment) mission data. Much of Australia is characterized by relatively small hydrological signals, with large precipitation gradients between the North and the South. These signals are better recovered using innovative GRACE processing techniques such as high-resolution mascon solutions, which may help overcome the deficiencies in the standard GRACE data processing and filtering methods. We will show the power of using regional and global mas con solutions to recover hydrological variations from 2003 to 2011, as well as the oceanic mass variations in the surrounding regions. We will compare the GRACE signals with state of the art hydrology and ocean general circulation models, precipitation, soil moisture and groundwater data sets. We especially emphasize the gravity signatures observed during the decadal drought in the Murray-Darling river basin and the early 2011 floods in North-Western Australia.

Carabajal, Claudia C.↗

Closed-loop control of meltpool temperature in directed energy deposition

The objective of this work is to mitigate flaw formation in powder and laser-based directed energy deposition (DED) additive manufacturing process through close-loop control of the meltpool temperature. In this work, the meltpool temperature was controlled by modulating the laser power based on feedback signals from a coaxial two-wavelength imaging pyrometer. The utility of closed-loop control in DED is demonstrated in the context of practically inspired trapezoid-shaped stainlesssteel parts (SS 316L). We demonstrate that parts built under closed-loop control have reduced variation in porosity and uniform microstructure compared to parts built under open-loop conditions. For example, post-process characterization showed that closed-loop processed parts had a volume percent porosity ranging from 0.036% to 0.043%. In comparison, open-loop processed parts had a larger variation in volume percent porosity ranging from 0.032% to 0.068%. Further, parts built with closed-loop processing depicted consistent dendritic microstructure. By contrast, parts built with open-loop processing showed microstructure heterogeneity with the presence of both dendritic and planar grains, which in turn translated to large variation in microhardness

36 MATERIALS SCIENCE↗

Model selection and signal extraction using Gaussian Process regression

We present a novel computational approach for extracting localized signals from smooth background distributions. We focus on datasets that can be naturally presented as binned integer counts, demonstrating our procedure on the CERN open dataset with the Higgs boson signature, from the ATLAS collaboration at the Large Hadron Collider. Our approach is based on Gaussian Process (GP) regression — a powerful and flexible machine learning technique which has allowed us to model the background without specifying its functional form explicitly and separately measure the background and signal contributions in a robust and reproducible manner. Unlike functional fits, our GP-regression-based approach does not need to be constantly updated as more data becomes available. We discuss how to select the GP kernel type, considering trade-offs between kernel complexity and its ability to capture the features of the background distribution. We show that our GP framework can be used to detect the Higgs boson resonance in the data with more statistical significance than a polynomial fit specifically tailored to the dataset. Finally, we use Markov Chain Monte Carlo (MCMC) sampling to confirm the statistical significance of the extracted Higgs signature.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

System and method for high power diode based additive manufacturing

The present disclosure relates to a system for performing an Additive Manufacturing (AM) fabrication process on a powdered material (PM) forming a substrate. The system uses a first optical subsystem to generate an optical signal comprised of electromagnetic (EM) radiation sufficient to melt or sinter a PM of the substrate. The first optical subsystem is controlled to generate a plurality of different power density levels, with a specific one being selected based on a specific PM forming a powder bed being used to form a 3D part. At least one processor controls the first optical subsystem and adjusts a power density level of the optical signal, taking into account a composition of the PM. A second optical subsystem receives the optical signal from the first optical subsystem and controls the optical signal to help facilitate melting of the PM in a layer-by-layer sequence of operations.

El-Dasher, Bassem S.↗

Feedback Augmented Sub-Ranging (FASR) Quantizer

This innovation is intended to reduce the size, power, and complexity of pipeline analog-to-digital converters (ADCs) that require high resolution and speed along with low power. Digitizers are important components in any application where analog signals (such as light, sound, temperature, etc.) need to be digitally processed. The innovation implements amplification of a sampled residual voltage in a switched capacitor amplifier stage that does not depend on charge redistribution. The result is less sensitive to capacitor mismatches that cause gain errors, which are the main limitation of such amplifiers in pipeline ADCs. The residual errors due to mismatch are reduced by at least a factor of 16, which is equivalent to at least 4 bits of improvement. The settling time is also faster because of a higher feedback factor. In traditional switched capacitor residue amplifiers, closed-loop amplification of a sampled and held residue signal is achieved by redistributing sampled charge onto a feedback capacitor around a high-gain transconductance amplifier. The residual charge that was sampled during the acquisition or sampling phase is stored on two or more capacitors, often equal in value or integral multiples of each other. During the hold or amplification phase, all of the charge is redistributed onto one capacitor in the feedback loop of the amplifier to produce an amplified voltage. The key error source is the non-ideal ratios of feedback and input capacitors caused by manufacturing tolerances, called mismatches. The mismatches cause non-ideal closed-loop gain, leading to higher differential non-linearity. Traditional solutions to the mismatch errors are to use larger capacitor values (than dictated by thermal noise requirements) and/or complex calibration schemes, both of which increase the die size and power dissipation. The key features of this innovation are (1) the elimination of the need for charge redistribution to achieve an accurate closed-loop gain of two, (2) a higher feedback factor in the amplifier stage giving a higher closed-loop bandwidth compared to the prior art, and (3) reduced requirement for calibration. The accuracy of the new amplifier is mainly limited by the sampling networks parasitic capacitances, which should be minimized in relation to the sampling capacitors.

Guilligan, Gerard↗

Fermilab PIP-II machine protection system digitized data noise elimination scheme and its FPGA implementation

In Fermilab's PIP-II machine protection system, beam loss signals from various detectors are digitized at 125 MS/s. Noise from both high-frequency sources and low-frequency 60 Hz AC power equipment can contaminate the data. To suppress noise across these ranges—especially 60 Hz and its harmonics, which overlap with beam loss signal frequencies—advanced digital processing beyond standard filtering is required. Several real-time functional blocks were simulated and tested on an FPGA: (1) a dual time-constant discharging integrator filter, (2) a de-ripple baseline extraction and storage block, and (3) a fast-recovery discharging integrator. The nonlinear IIR integrator filter removes high-frequency noise and feeds into the baseline extractor. Upon detecting abrupt beam loss, it switches to a longer time constant to prevent baseline distortion. The de-ripple block calculates a valid baseline by averaging over multiple 60 Hz periods, storing results in a 4096-word FPGA RAM. This baseline is subtracted from raw data before integration by the fast-recovery block, which resets quickly after use. All blocks achieved expected performance and were successfully implemented on a low-cost FPGA.

Wu, Jinyuan [Fermilab]↗

sdt (Solar Data Tools) [SWR-25-130]

Solar Data Tools (sdt) is an open-source Python library for analyzing PV power (and irradiance) time-series data. It was developed to enable analysis of unlabeled PV data, i.e. with no model, no meteorological data, and no performance index required, by taking a statistical signal processing approach in the algorithms used in the package’s main data processing pipeline. Solar Data Tools empowers PV system fleet owners or operators to analyze system performance a hundred times faster even when they only have access to the most basic data stream—power output of the system.

Meyers-Im, Bennet [National Laboratory of the Rock↗

Advanced technology for a satellite multichannel demultiplexer/demodulator

Satellite on-board processing is needed to efficiently service multiple users while at the same time minimizing earth station complexity. The processing satellite receives a wideband uplink at 30 GHz and down-converts it to a suitable intermediate frequency. A multichannel demultiplexer then separates the composite signal into discrete channels. Each channel is then demodulated by bulk demodulators, with the baseband signals routed to the downlink processor for retransmission to the receiving earth stations. This type of processing circumvents many of the difficulties associated with traditional bent-pipe repeater satellites. Uplink signal distortion and interference are not retransmitted on the downlink. Downlink power can be allocated in accordance with user needs, independent of uplink transmissions. This allows the uplink users to employ different data rates as well as different modulation and coding schemes. In addition, all downlink users have a common frequency standard and symbol clock on the satellite, which is useful for network synchronization in time division multiple access schemes. The purpose of this program is to demonstrate the concept of an optically implemented multichannel demultiplexer (MCD). A proof-of-concept (POC) model has been developed which has the ability to receive a 40 MHz wide composite signal consisting of up to 1000 40 kHz QPSK modulated channels and perform the demultiplexing process. In addition a set of special test equipment (STE) has been configured to evaluate the performance of the POC model. The optical MCD is realized as an acousto-optic spectrum analyzer utilizing the capability of Bragg cells to perform the required channelization. These Bragg cells receive an optical input from a laser source and an RF input (the signal). The Bragg interaction causes optical output diffractions at angles proportional to the RF input frequency. These discrete diffractions are optically detected and output to individual demodulators for baseband conversion. Optimization of the MCD design was conducted in order to achieve a compromise between two opposing sources of signal degradation: adjacent channel interference and intersymbol interference. The system was also optimized to allow simple, inexpensive ground stations communications with the MCD. These design goals led to the realization of a POC MCD which demonstrates the demultiplexing function with minimal signal degradation. Performance evaluation results using the STE equipment indicate that the dynamic range of the demultiplexer in the presence of adjacent and multiple channel loading is 40 - 50 dB. Measured bit error rate (BER) probabilities varied from the predicted theoretical results by one dB or less. The performance of the proof-of-concept model indicate that the development of a space qualified optically implemented MCD are feasible. The advantages to such an implementation include reduced size, weight and power and increased reliability when compared with electronic approaches. All of these factors are critical to on-board satellite processors. Further optimization can be conducted which trade ground station complexity and MCD performance to achieve desired system results.

Abramovitz, Irwin J.↗

Synchronized Docking System

A synchronized target subsystem for use in an automated docking system for docking a chase vehicle with a target vehicle wherein the chase vehicle is provided with a video camera for feeding digitized frames to an image processing unit which feeds signals to a control circuit. The control circuit turns on the video camera to digitize a background frame which will include the target vehicle. After the camera grabs the background frame the control circuit turns on a light, which is carried by carried on the chase vehicle and aimed at the target vehicle, and signals the video camera to digitize a foreground frame. A light sensing circuit on the target vehicle receives the light from the chase vehicle and connects a power supply to lights on the target vehicle such that when the foreground frame is digitized the Lights on the target vehicle will show in the foreground frame. Die image processing unit subtracts the background frame from the foreground frame and provides a docking signal.

Richard T Howard↗

Using Piezoelectric Devices to Transmit Power through Walls

A method denoted wireless acoustic-electric feed-through (WAEF) has been conceived for transmitting power and/or data signals through walls or other solid objects made of a variety of elastic materials that could be electrically conductive or nonconductive. WAEF would make it unnecessary to use wires, optical fibers, tubes, or other discrete wall-penetrating signal-transmitting components, thereby eliminating the potential for structural weakening or leakage at such penetrations. Avoidance of such penetrations could be essential in some applications in which maintenance of pressure, vacuum, or chemical or biological isolation is required. In a basic WAEF setup, a transmitting piezoelectric transducer on one side of a wall would be driven at resonance to excite ultrasonic vibrations in the wall. A receiving piezoelectric transducer on the opposite side of the wall would convert the vibrations back to an ultrasonic AC electric signal, which would then be detected and otherwise processed in a manner that would depend on the modulation (if any) applied to the signal and whether the signal was used to transmit power, data, or both. An electromechanical-network model has been derived as a computationally efficient means of analyzing and designing a WAEF system. This model is a variant of a prior model, known in the piezoelectric-transducer art as Mason's equivalent-circuit model, in which the electrical and mechanical dynamics, including electromechanical couplings, are expressed as electrical circuit elements that can include inductors, capacitors, and lumped-parameter complex impedances. The real parts of the complex impedances are used to account for dielectric, mechanical, and coupling losses in all components (including all piezoelectric-transducer, wall, and intermediate material layers). In an application to a three-layer piezoelectric structure, this model was shown to yield the same results as do solutions of the wave equations of piezoelectricity and acoustic propagation in their full complexity.

Sherrit, Stewart↗

Impulse Response Measurements Over Space-Earth Paths Using the GPS Coarse/Acquisition Codes

The impulse responses of radio transmission channels over space-earth paths were measured using the course/acquisition code signals from the Global Positioning System of satellites. The data acquisition system and signal processing techniques used to develop the impulse responses are described. Examples of impulse response measurements are presented. The results indicate that this measurement approach enables detection of multipath signals that are 20 dB or more below the power of the direct arrival. Channel characteristics that could be investigated with additional measurements and analyses are discussed.

SIGNAL PROCESSING↗

Receiver Signal to Noise Ratios for IPDA Lidars Using Sine-wave and Pulsed Laser Modulation and Direct Detections

Integrated path differential absorption (IPDA) lidar can be used to remotely measure the column density of gases in the path to a scattering target [1]. The total column gas molecular density can be derived from the ratio of the laser echo signal power with the laser wavelength on the gas absorption line (on-line) to that off the line (off-line). 80th coherent detection and direct detection IPDA lidar have been used successfully in the past in horizontal path and airborne remote sensing measurements. However, for space based measurements, the signal propagation losses are often orders of magnitude higher and it is important to use the most efficient laser modulation and detection technique to minimize the average laser power and the electrical power from the spacecraft. This paper gives an analysis the receiver signal to noise ratio (SNR) of several laser modulation and detection techniques versus the average received laser power under similar operation environments. Coherent detection [2] can give the best receiver performance when the local oscillator laser is relatively strong and the heterodyne mixing losses are negligible. Coherent detection has a high signal gain and a very narrow bandwidth for the background light and detector dark noise. However, coherent detection must maintain a high degree of coherence between the local oscillator laser and the received signal in both temporal and spatial modes. This often results in a high system complexity and low overall measurement efficiency. For measurements through atmosphere the coherence diameter of the received signal also limits the useful size of the receiver telescope. Direct detection IPDA lidars are simpler to build and have fewer constraints on the transmitter and receiver components. They can use much larger size 'photon-bucket' type telescopes to reduce the demands on the laser transmitter. Here we consider the two most widely used direct detection IPDA lidar techniques. The first technique uses two CW seeder lasers, one on-line and one offline that are intensity modulated by two different frequency sine-waves signals before being amplified by a common laser amplifier. The receiver uses narrowband amplitude demodulation, or lock-in, Signal processing at the given laser modulation frequencies [3,4]. The laser transmitter operates in a quasi CW mode with the peak power equal to twice the average power. The on-line and off-line lasers can be transmitted at the same time without interference. Another direct detection technique uses a low duty cycle pulsed laser modulation [5,6] with the laser wavelengths alternating between on-line and off-line on successive pulses. The receiver uses time resolved detection and can also provide simultaneous target range measurement. With a lower laser duty cycle it requires a much higher peak laser power for the same average power.

Sun, Xiaoli↗

Frequency-domain computing using nonlinear acoustic-wave device on lithium niobate

Abstract Multiply-accumulation are crucial computing operations in signal processing, numerical simulations, and machine learning. In recent years, optical analog approaches have demonstrated higher computing performance and better power efficiency than their digital counterparts. However, analog computing chips usually need large areas and complex structures for parallel computing, as a single device element only executes one computing operation at a single time. Here, we demonstrate frequency-domain computing using the nonlinear acoustic-wave devices on lithium niobate, featuring a normalized external second-harmonic generation conversion efficiency of ~ 5.7 × 10-4 W-1. The second-order sum-frequency nonlinear process of lithium niobate enables multiplication of inputs encoded in the frequency domain. Compared to the analog schemes, our device features a notably simpler design, and nanofabrication requires only one lift-off. Using a single acoustic-wave device within an area of 0.03 mm2, we can simultaneously conduct over 130,000 multiply-accumulation operations. Our acoustic-wave device shows applications in real and complex vector convolutions and image processing. This demonstration sets the stage for experimental realizations into frequency-domain integrated nonlinear acoustic computing systems, potentially shaping future developments in acoustic neural networks and quantum computing.

chai, mingzhao (ORCID:0009000466226341)↗

Rayleigh Scattering Diagnostic for Simultaneous Measurements of Dynamic Density and Velocity

A flow diagnostic technique based on the molecular Rayleigh scattering of laser light is used to obtain dynamic density and velocity data in turbulent flows. The technique is based on analyzing the Rayleigh scattered light with a Fabry-Perot interferometer and recording information about the interference pattern with a multiple anode photomultiplier tube (PMT). An artificial neural network is used to process the signals from the PMT to recover the velocity time history, which is then used to calculate the velocity power spectrum. The technique is illustrated using simulated data. The results of an experiment to measure the velocity power spectrum in a low speed (100 rn/sec) flow are also presented.

Seasholtz, Richard G.↗