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At least 271 records · Page 15

Comparative proteome approach demonstrates that platelet-derived growth factor C and D efficiently induce proliferation while maintaining multipotency of hMSCs

This is the first study that comprehensively describes the effects of the platelet-derived growth factor (PDGF) isoforms C and D during in vitro expansion of human mesenchymal stem cells (hMSCs). Our results show that PDGFs can enhance proliferation of hMSCs without affecting their multipotency. It is of great value to culture and expand hMSCs in a safe and effective manner without losing their multipotency for manipulation and further development of cell-based therapies. Moreover, differential effects of PDGF isoforms have been observed on lineage-specific differentiation induced by BMP2 and Vitamin D3. Based on label-free LC-based quantitative proteomics approach we have furthermore identified specific pathways induced by PDGFs during the proliferation process, showing the importance of bioinformatics tools to study cell function. - Highlights: • PDGFs (C and D) significantly increased the number of multipotent undifferentiated hMSCs. • Enhanced proliferation did not impair the ability to undergo lineage-specific differentiation. • Proteomic analysis confirmed the overall signatures of the ‘intact’ cells.

60 APPLIED LIFE SCIENCES↗

Assessing microbial community patterns under incipient soil formation from basalt

Microbial dynamics drive the biotic machinery of early soil evolution. However, integrated knowledge of microbial community establishment, functional associations, and community assembly processes in incipient soil is lacking. This study presents a novel approach of combining microbial phylogenetic profiling, functional predictions, and community assembly processes to analyze drivers of microbial community establishment in an emerging soil system. Rigorous sub-meter sampling of a basalt-soil lysimeter after two years of irrigation revealed the contrasting depth-dependent distribution patterns of soil parameters and community diversity. Phylogenetic analysis of 16S rRNA gene indicated the presence of diverse bacterial and archaeal phyla, with high relative abundance of Actinobacteria on the surface and a consistently high abundance of Proteobacteria at all depths. Predicted functional gene analysis suggested dominant autotrophy signatures, evidence of an incomplete nitrogen cycle, and predicted enzymes of iron (III) to iron (II) conversion followed by intracellular uptake, transport, and regulation. Null modeling revealed microbial community assembly predominantly governed by variable selection. The relative influence of the variable selection decreased with depth, indicating unique and relatively harsh environmental conditions near the surface and more benign conditions with depth. Additionally, high levels of dispersal influenced community composition near the center of the domain, suggesting that spatial processes interact with deterministic selection imposed by the environment. Characterizing the complexity in incipient soils can advance research insights about microbial community diversity and assembly in other incipient systems (e.g., transformation of marginal soils, or soils recovering from disturbances such as fire, erosion, intensive agricultural practices, or contamination).

Sengupta, Aditi↗

Extreme ultraviolet spectroscopy and modeling of Cu on the SSPX Spheromak and laser plasma 'Sparky'

Impurities play a critical role in magnetic fusion research. In large quantities, impurities can cool and dilute plasma creating problems for achieving ignition and burn; however in smaller amounts the impurities could provide valuable information about several plasma parameters through the use of spectroscopy. Many impurity ions radiate within the extreme ultraviolet (EUV) range. Here, we report on spectra from the silver flat field spectrometer, which was implemented at the Sustained Spheromak Physics experiment (SSPX) to monitor ion impurity emissions. The chamber within the SSPX was made of Cu, which makes M-shell Cu a prominent impurity signature. The Spect3D spectral analysis code was utilized to identify spectral features in the range of 115-315 A and to more fully understand the plasma conditions. A second set of experiments was carried out on the compact laser-plasma x-ray/EUV facility 'Sparky' at UNR, with Cu flat targets used. The EUV spectra were recorded between 40-300 A and compared with results from SSPX.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Conical intersection and coherent vibrational dynamics in alkyl iodides captured by attosecond transient absorption spectroscopy

The photodissociation dynamics of alkyl iodides along the C–I bond are captured by attosecond extreme-ultraviolet (XUV) transient absorption spectroscopy employing resonant ∼20 fs UV pump pulses. The methodology of previous experiments on CH3I [Chang et al., J. Chem. Phys. 154, 234301 (2021)] is extended to the investigation of a C–I bond-breaking reaction in the dissociative A-band of C2H5I, i-C3H7I, and t-C4H9I. Probing iodine 4d core-to-valence transitions in the XUV enables one to map wave packet bifurcation at a conical intersection in the A-band as well as coherent vibrations in the ground state of the parent molecules. Analysis of spectroscopic bifurcation signatures yields conical intersection crossing times of 15 ± 4 fs for CH3I, 14 ± 5 fs for C2H5I, and 24 ± 4 fs for i-C3H7I and t-C4H9I, respectively. Observations of coherent vibrations, resulting from a projection of A-band structural dynamics onto the ground state by resonant impulsive stimulated Raman scattering, indirectly reveal multimode C–I stretch and CCI bend vibrations in the A-bands of C2H5I, i-C3H7I, and t-C4H9I.

Chang, Kristina F. (ORCID:000000022315039X)↗

Kepler-93b: A terrestrial world measured to within 120 km, and a test case for a new Spitzer observing mode

We present the characterization of the Kepler-93 exoplanetary system, based on three years of photometry gathered by the Kepler spacecraft. The duration and cadence of the Kepler observations, in tandem with the brightness of the star, enable unusually precise constraints on both the planet and its host. We conduct an asteroseismic analysis of the Kepler photometry and conclude that the star has an average density of 1.652 ± 0.006 g cm{sup –3}. Its mass of 0.911 ± 0.033 M{sub ☉} renders it one of the lowest-mass subjects of asteroseismic study. An analysis of the transit signature produced by the planet Kepler-93b, which appears with a period of 4.72673978 ± 9.7 × 10{sup –7} days, returns a consistent but less precise measurement of the stellar density, 1.72{sub −0.28}{sup +0.02} g cm{sup –3}. The agreement of these two values lends credence to the planetary interpretation of the transit signal. The achromatic transit depth, as compared between Kepler and the Spitzer Space Telescope, supports the same conclusion. We observed seven transits of Kepler-93b with Spitzer, three of which we conducted in a new observing mode. The pointing strategy we employed to gather this subset of observations halved our uncertainty on the transit radius ratio R{sub P} /R{sub *}. We find, after folding together the stellar radius measurement of 0.919 ± 0.011 R{sub ☉} with the transit depth, a best-fit value for the planetary radius of 1.481 ± 0.019 R{sub ⊕}. The uncertainty of 120 km on our measurement of the planet's size currently renders it one of the most precisely measured planetary radii outside of the solar system. Together with the radius, the planetary mass of 3.8 ± 1.5 M{sub ⊕} corresponds to a rocky density of 6.3 ± 2.6 g cm{sup –3}. After applying a prior on the plausible maximum densities of similarly sized worlds between 1 and 1.5 R{sub ⊕}, we find that Kepler-93b possesses an average density within this group.

79 ASTRONOMY AND ASTROPHYSICS↗

COUPLED HBO AND NBO VARIATIONS IN THE Z SOURCE GX 5-1: INNER ACCRETION DISK AS THE LOCATION OF QPOs

The simultaneous and coupled evolution of horizontal branch oscillation (HBO) and normal branch oscillation (NBO) in Z-type sources suggests that the production of HBO is connected to NBO and is caused by changes in the physical/radiative properties of the inner accretion disk, although there is a lack of substantial spectral evidence to support this. In this Letter, we present the results of an analysis of an RXTE observation of the Z source GX 5-1, where the 6 Hz NBO is simultaneously detected along with an HBO at 51 Hz. The variations in the intensity and the associated power density spectrum indicate that the HBO and NBO are strongly coupled, originating from the same location in the inner accretion disk. The absence of HBO and NBO in the lower energy bands, an increase in the rms amplitude with energy, and a smooth transition among them suggest that they are produced in the hot inner regions of the accretion disk. Based on a spectral analysis, we found a signature of changing or physically modified inner disk front during the coupled HBO and NBO evolution. We explore the various models to explain the observed phenomenon and propose that the NBO is affiliated to the oscillations in the thick/puffed-up inner region of the accretion disk.

79 ASTRONOMY AND ASTROPHYSICS↗

Spectroscopy of 33 Mg with knockout reactions

In this work, the structure of 33 Mg was investigated by means of two knockout reactions, one-neutron removal from 34 Mg and one-proton removal from 34 Al. Using comparative analysis of the population of observed excited states in the residual 33 Mg, the nature of these states can be deciphered. In addition, the long-standing controversy about the parity of the 33 Mg ground state is resolved using momentum distribution analysis, showing a clear signature for negative parity. Partial cross section measurements are compared with the results of eikonal reaction theory combined with large-scale shell model calculations of this complex nucleus located in the island of inversion, where configuration mixing plays a major role.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

Search in diphoton and dielectron final states for displaced production of Higgs or Z bosons with the ATLAS detector in s = 13 TeV p p collisions

A search is presented for displaced production of Higgs bosons or Z bosons, originating from the decay of a neutral long-lived particle (LLP) and reconstructed in the decay modes H → γ γ and Z → ee . The analysis uses the full Run 2 dataset of proton-proton collisions delivered by the LHC at an energy of $\sqrt{s}$ = 13 TeV between 2015 and 2018 and recorded by the ATLAS detector, corresponding to an integrated luminosity of 139 fb -1 . Exploiting the capabilities of the ATLAS liquid argon calorimeter to precisely measure the arrival times and trajectories of electromagnetic objects, the analysis searches for the signature of pairs of photons or electrons which arise from a common displaced vertex and which arrive after some delay at the calorimeter. The results are interpreted in a gauge-mediated supersymmetry breaking model with pair-produced Higgsinos that decay to LLPs, and each LLP subsequently decays into either a Higgs boson or a Z boson. The final state includes at least two particles that escape direct detection, giving rise to missing transverse momentum. No significant excess is observed above the background expectation. The results are used to set upper limits on the cross section for Higgsino pair production, up to a $\bar{χ}^0_1$ mass of 369 (704) GeV for decays with 100% branching ratio of $\bar{χ}^0_1$ to Higgs (Z) bosons for a $\bar{χ}^0_1$ lifetime of 2 ns. A model-independent limit is also set on the production of pairs of photons or electrons with a significant delay in arrival at the calorimeter.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

TESTING OF THE DUAL ROTARY FILTER SYSTEM

The Savannah River National Laboratory (SRNL) installed and tested two hydraulically connected SpinTek rotary microfilter (RMF) units to determine the behavior of a multiple filter system. Both units were successfully controlled by a control scheme written in DELTA-V architecture by Savannah River Remediation (SRR) Process Control Engineering personnel. The control system was tuned to provide satisfactory response to changing conditions during the operation of the multi-filter system. Stability was maintained through the startup and shutdown of one of the filter units while the second was still in operation. The installation configuration originally proposed by the Small Colum Ion Exchange (SCIX) project of independent filter and motor mountings may be susceptible to vibration. Significant stiffening of the filter and motor mounts was required to minimize the vibration. Alignment of the motor to the filter was a challenge in this test configuration. The deployment configuration must be easy to manipulate and allow for fine adjustment. An analysis of the vibration signature of the test system identified critical speeds. Whether it corresponds to the resonance frequency of a rotor radial vibration mode that was excited by rotor unbalance is uncertain based upon the measurements. A relative motion series should be completed on the filter with the final shaft configuration to determine if the resonances exist in the final filter design. The instrumentation selected for deployment, including the concentrate discharge control valve and flow meters, performed well. Automation of the valve control integrated well with the control scheme and when used in concert with the other control variables, allowed automated control of the dual RMF system. The one area of concern with the instrumentation was the condition resulting when the filtrate flow meter operated with less than three gpm. This low flow was at the lower range of performance for the flow meter. This should not be an issue in deployment where the desired flow rate will be within the normal operating range of the meter. Testing demonstrated that the use of a flexible line for the filtrate discharge is highly desired at the outlet of the rotary union to transition to the system piping. Isolating the vibration from the rotary union will significantly improve the lifetime of the seals. Methods to monitor and isolate individual filters should be considered during deployment. The ability to diagnose issues and isolate individual filters would allow isolation prior to failure. Thus, filters may be cleaned or repaired instead of requiring complete replacement if the condition were to continue unnoticed. Isolating the filtrate line of each filter during startup will minimize the premature buildup of solids on the filter disks. Several tests have shown that the method of filter startup can improve performance lifetime of the filters. The installation must factor in an air inlet for the draining of a filter that does not involve a reverse flow through the filter disks. The reverse flow may cause deformation of the disks or may damage other components of the filters themselves.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Rotation-Enabled 7-Degree of Freedom Seismometer for Geothermal Resource Development. Phase 1 Final Report

Under this Department of Energy (DOE) grant, A-Tech Corporation d.b.a. Applied Technology Associates (ATA), seeks to develop a seven-degree-of-freedom (7-DOF) seismic measurement tool for high-temperature geothermal applications. The Rotational-Enabled 7-DOF Seismometer includes a conventional tri-axial accelerometer, a conventional pressure sensor or hydrophone, and a tri-axial rotational sensor. The rotational sensing capability is novel, based upon ATA's innovative research in rotational sensing technologies. The geothermal industry requires tools for high-precision seismic monitoring of crack formation associated with Enhanced Geothermal System (EGS) stimulation activity. Currently, microseismic monitoring is conducted by deploying many seismic tools at different depth levels along a 'string' within drilled observation wells. Costs per string can be hundreds of thousands of dollars. Processing data from the spatial arrays of linear seismometers allows back-projection of seismic wave states. In contrast, a Rotational-Enabled 7-DOF Seismometer would simultaneously measure p-wave velocity, s-wave velocity, and incident seismic wave direction all from a single point measurement. In addition, the Rotational-Enabled 7-DOF Seismometer will, by its nature, separate p- and s-waves into different data streams, simplifying signal processing and facilitating analysis of seismic source signatures and geological characterization. By adding measurements of three additional degrees-of-freedom at each level and leveraging the information from this new seismic observable, it is likely that an equally accurate picture of subsurface seismic activity could be garnered with fewer levels per hole. The key cost savings would come from better siting of the well due to increased information content and a decrease in the number of confirmation wells drilled, also due to the increase in information per well. Improved seismic tools may also increase knowledge, understanding, and confidence, thus removing some current blocks to feasibility and significantly increasing access to potential geothermal sites. During the Phase 1 effort summarized in this final report, the ATA Team modeled and built two TRL 3 proof-of-concept test units for two competing rotational sensor technologies. The two competing technologies were based on ATA's angular rate and angular displacement measurement technologies; Angular rate: ATA's Magnetohydrodynamic Angular Rate Sensor (Seismic MHD); and Angular displacement: ATA's Low Frequency Improved Torsional Seismometer (LFITS). In order to down-select between these two technologies and formulate a go / no go decision, the ATA Team analyzed and traded scientific performance requirements and market constraints against sensor characteristics and components, acquiring field data where possible to validate the approach and publishing results from these studies of rotational technology capability. Based on the results of Phase 1, the ATA Team finds that the Seismic MHD (SMHD) technology is the best choice for enabling rotational seismometry and significant technical potential exists for micro-seismic monitoring using a downhole 7-DOF device based on the SMHD. Recent technical papers and field data confirm the potential of rotational sensing for seismic mapping, increasing confidence that cost-reduction benefits are achievable for EGS. However, the market for geothermal rotational sensing is small and undeveloped. As a result, this report recommends modifying the Phase 2 plan to focus on prototype development aimed at partnering with early adopters within the geothermal industry and the scientific research community. The highest public benefit will come from development and deployment of a science-grade SMHD rotational seismometer engineered for geothermal downhole conditions and an integrated test tool for downhole measurements at active geothermal test sites.

15 GEOTHERMAL ENERGY↗

National Security Science and Technology Initiative: Air Cargo Screening

The non-intrusive inspection (NII) of consolidated air cargo carried on commercial passenger aircraft continues to be a technically challenging, high-priority requirement of the Department of Homeland Security's Science and Technology Directorate (DHS S&T), the Transportation Security Agency and the Federal Aviation Administration. The goal of deploying a screening system that can reliably and cost-effectively detect explosive threats in consolidated cargo without adversely affecting the flow of commerce will require significant technical advances that will take years to develop. To address this critical National Security need, the Battelle Memorial Institute (Battelle), under a Cooperative Research and Development Agreement (CRADA) with four of its associated US Department of Energy (DOE) National Laboratories (Oak Ridge, Pacific Northwest, Idaho, and Brookhaven), conducted a research and development initiative focused on identifying, evaluating, and integrating technologies for screening consolidated air cargo for the presence of explosive threats. Battelle invested $8.5M of internal research and development funds during fiscal years 2007 through 2009. The primary results of this effort are described in this document and can be summarized as follows: (1) Completed a gap analysis that identified threat signatures and observables, candidate technologies for detection, their current state of development, and provided recommendations for improvements to meet air cargo screening requirements. (2) Defined a Commodity/Threat/Detection matrix that focuses modeling and experimental efforts, identifies technology gaps and game-changing opportunities, and provides a means of summarizing current and emerging capabilities. (3) Defined key properties (e.g., elemental composition, average density, effective atomic weight) for basic commodity and explosive benchmarks, developed virtual models of the physical distributions (pallets) of three commodity types and three explosive benchmarks combinations, and conducted modeling and simulation studies to begin populating the matrix of commodities, threats, and detection technologies. (4) Designed and fabricated basic (homogeneous) commodity test pallets and fabricated inert stimulants to support experiments and to validate modeling/simulation results. (5) Developed/expanded the team's capabilities to conduct full-scale imaging (neutron and x-ray) experiments of air cargo commodities and explosive benchmarks. (6) Conducted experiments to improve the collection of trace particles of explosives from a variety of surfaces representative of air cargo materials by means of mechanical (air/vibration/pressure), thermal, and electrostatic methods. Air cargo screening is a difficult challenge that will require significant investment in both research and development to find a suitable solution to ensure the safety of passengers without significantly hindering the flow of commodities. The initiative funded by Battelle has positioned this group to make major contributions in meeting the air cargo challenge by developing collaborations, developing laboratory test systems, improving knowledge of the challenges (both technical and business) for air cargo screening, and increasing the understanding of the capabilities for current inspection methods (x-ray radiography, x-ray backscatter, etc.) and potential future inspection methods (neutron radiography, fusion of detector modalities, advanced trace detection, etc.). Lastly, air cargo screening is still an issue that will benefit from collaboration between Department of Energy Laboratories and Battelle. On January 7, 2010, DHS Secretary Napolitano joined White House Press Secretary Robert Gibbs and Assistant to the President for Counterterrorism and Homeland Security John Brennan to announce several recommendations DHS has made to the President for improving the technology and procedures used to protect air travel from acts of terrorism. (This announcement followed the 25 Dec'09 Delta/Northwest Airlines Flight 253 terror attack.) Secretary Napolitano outlined five recommendations DHS will pursue to enhance the safety of the traveling public. One of the five recommendations, read as follows: 'Establish a partnership on aviation security between DHS and the Department of Energy and its National Laboratories in order to develop new and more effective technologies to deter and disrupt known threats and proactively anticipate and protect against new ways by which terrorists could seek to board an aircraft.' In conclusion, it appears very timely that Battelle and its DOE lab partners initiated a serious collaboration on the air cargo topic, and that we should continue to work toward future collaboration in response to the government's needs.

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEF↗

A CIRCULAR-CYLINDRICAL FLUX-ROPE ANALYTICAL MODEL FOR MAGNETIC CLOUDS

We present an analytical model to describe magnetic flux-rope topologies. When these structures are observed embedded in Interplanetary Coronal Mass Ejections (ICMEs) with a depressed proton temperature, they are called Magnetic Clouds (MCs). Our model extends the circular-cylindrical concept of Hidalgo et al. by introducing a general form for the radial dependence of the current density. This generalization provides information on the force distribution inside the flux rope in addition to the usual parameters of MC geometrical information and orientation. The generalized model provides flexibility for implementation in 3D MHD simulations. Here, we evaluate its performance in the reconstruction of MCs in in situ observations. Four Earth-directed ICME events, observed by the Wind spacecraft, are used to validate the technique. The events are selected from the ICME Wind list with the magnetic obstacle boundaries chosen consistently with the magnetic field and plasma in situ observations and with a new parameter (EPP, the Electron Pitch angle distribution Parameter) which quantifies the bidirectionally of the plasma electrons. The goodness of the fit is evaluated with a single correlation parameter to enable comparative analysis of the events. In general, at first glance, the model fits the selected events very well. However, a detailed analysis of events with signatures of significant compression indicates the need to explore geometries other than the circular-cylindrical. An extension of our current modeling framework to account for such non-circular CMEs will be presented in a forthcoming publication.

79 ASTRONOMY AND ASTROPHYSICS↗

Identification and mapping of coal refuse banks and other targets in the anthracite region

ERTS-1 MSS data covering parts of Pennsylvania's southern and eastern middle anthracite coal fields were studied to determine how well accumulations of coal refuse could be identified and mapped by computer analysis and processing. Spectral signatures of coal refuse targets were similar to water, but had higher reflectances in all channels. Relative reflectances were in the order 4 5 or = 6 7. Although no underflight photography was at hand to judge mapping success, correlation was made, with 1:24,000 scale U.S.G.S. maps dated 1947 and 1948. Coal refuse targets correlated well with existing maps.

Borden, F. Y.↗

Earth observations

The development of the Earth Resources Technology Satellites for making earth observations from space is discussed. The programmatic elements of the ERTS program are defined. The specific results of the program which are considered are as follows: (1) analysis of snowmelt runoff, (2) analysis of Mississippi River floods of 1973, (3) global ice surveys with an electrically scanning microwave radiometer, (4) the microwave signature of snow fields, (5) analysis of strip mining activities, (6) sea surface studies using microwave techniques during the Bering Sea experiment, (7) cloud types and measurement of rainfall, and (8) performance of various remote sensors.

Source record↗

A new use of high resolution magnetograms

Ground-based solar magnetograms are frequently in error by as much as twenty percent and contribute to the poor correlation between magnetic changes and solar flares. High resolution measurement of the magnetic field component, which is normal to the photosphere and measured at photospheric height, can be used to construct a magnetic flux partition function F. Therefore, dF/dt is an EMF which drives atmospheric currents in reconnecting solar active regions. With a high quality magnetograph, the solar probe can be used to obtain good estimates of F and dF/dt and thereby the energy stored as induced solar atmospheric currents during quiescent interflare periods. Should a flare occur during a favorable observing period, the present method of analysis should show characteristic signatures in F, DF/dt, and especially, in the stored flux computed from dF/dt.

Baum, P. J.↗

Comparison of techniques for discriminating hydrothermal alteration minerals with Airborne Imaging Spectrometer data

Hydrothermal alteration mineralogy in the Tybo mining district of Nevada has been mapped on the basis of high spectral and spatial resolution Airborne Imaging Spectrometer (AIS) data, using band ratios, principal component analysis, and a signature-matching algorithm to delineate the alteration zones and limestone foundations. The signature-matching algorithm is found to be the most effective method of discriminating alteration minerals, and is noted to be able to identify mineralogy by matching AIS image spectra with library reference spectra. AIS bands in the 2048-2337-nm portion of the spectrum accounted for the greatest amount of variance.

Feldman, Sandra C.↗