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At least 271 records · Page 15

High-efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda)

Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultivation conditions and different disarmed strains of Agrobacterium to improve transformation. Additional copies of virulence genes were added to three common disarmed strains. These extra virulence genes included either a constitutively active virG or extra copies of virG and virB, both from pTiBo542. In experiments with Norway spruce, we increased transformation efficiencies 1000-fold from initial experiments where little or no transient expression was detected. Over 100 transformed lines expressing the marker gene beta-glucuronidase (GUS) were generated from rapidly dividing embryogenic suspension-cultured cells co-cultivated with Agrobacterium. GUS activity was used to monitor transient expression and to further test lines selected on kanamycin-containing medium. In loblolly pine, transient expression increased 10-fold utilizing modified Agrobacterium strains. Agrobacterium-mediated gene transfer is a useful technique for large-scale generation of transgenic Norway spruce and may prove useful for other conifer species.

NASA Discipline Plant Biology↗

The Science Goals of NASA's Exploration Initiative

The recently released policy directive, "A Renewed Spirit of Discovery: The President's Vision for U. S. Space Exploration," seeks to advance the U. S. scientific, security and economic interest through a program of space exploration which will robotically explore the solar system and extend human presence to the Moon, Mars and beyond. NASA's implementation of this vision will be guided by compelling questions of scientific and societal importance, including the origin of our Solar System and the search for life beyond Earth. The Exploration Roadmap identifies four key targets: the Moon, Mars, the outer Solar System, and extra-solar planets. First, a lunar investigation will set up exploration test beds, search for resources, and study the geological record of the early Solar System. Human missions to the Moon will serve as precursors for human missions to Mars and other destinations, but will also be driven by their support for furthering science. The second key target is the search for past and present water and life on Mars. Following on from discoveries by Spirit and Opportunity, by the end of the decade there will have been an additional rover, a lander and two orbiters studying Mars. These will set the stage for a sample return mission in 2013, increasingly complex robotic investigations, and an eventual human landing. The third key target is the study of underground oceans, biological chemistry, and their potential for life in the outer Solar System. Beginning with the arrival of Cassini at Saturn in July 2004 and a landing on Titan in 2006, the next decade will see an extended investigation of the Jupiter icy moons by a mission making use of Project Prometheus, a program to develop space nuclear power and nuclear-electric propulsion. Finally, the search for Earth-like planets and life includes a series of telescopic missions designed to find and characterize extra-solar planets and search them for evidence of life. These missions include HST and Spitzer, operating now; Kepler, SIM, JWST, and TPF, currently under development; and the vision missions, Life Finder and Planet Imager, which will possibly be constructed in space by astronauts.

Gardner, Jonathan P.↗

Speed of mental processing in the middle of the night

This study aimed to determine whether human mental processing actually slows down during the night hours, separately from the previously documented microsleeps, lapses in attention, and general slowing of motor responses. Eighteen healthy young adults were studied during 36 hours of constant wakeful bedrest. Every 2 hours, they performed a logical reasoning task. Items phrased in the negative voice took reliably longer to respond to than items phrased in the positive voice, indicating the need for more mental processing in those items. By subtracting "negative" from "positive" reaction times at each time of day, we were able to plot a circadian rhythm in the time taken for this extra mental processing to be done separately from microsleeps, psychomotor slowing, and inattention. The extra mental processing took longer at night and on the day following sleep loss than it did during the day before the sleep loss, suggesting that human mental processing slows down during the night under sleep deprivation.

Non-NASA Center↗

Prostaglandin E2 (PGE2) and risedronate was superior to PGE2 alone in maintaining newly added bone in the cortical bone site after withdrawal in older intact rats

The objects of this study were (1) to determine the effects of risedronate (Ris) and prostaglandin E2 (PGE2) alone and in combination, on tibial diaphyses of older intact female rats; and (2) to observe the fate of any extra bone if formed after withdrawal of the treatment. Nine-month-old female Sprague-Dawley rats were treated with 6 mg of PGE2/kg/day, 1 or 5 micrograms of Ris/kg twice a week, or 6 mg of PGE2/kg/day plus 1 or 5 micrograms of Ris/kg twice a week for the first 60 days and followed by vehicle injections for another 60 days. Cross-sections of double fluorescent labeled, undecalcified tibial diaphyses proximal to the tibiofibular junction were processed for histomorphometry. We found that: (1) neither the 1 microgram nor the 5 micrograms of Ris treatment in the 60-day on/60-day off group showed any histomorphometric differences from age-related controls; (2) while the 60 days of PGE2 treatment added extra cortical bone (6%) on the tibial shaft (due to stimulation of periosteal, endocortical, and marrow trabecular bone formation), the new endocortical and most of the new marrow trabecular bone were lost when treatment was withdrawn; however, the new periosteal bone remained; (3) PGE2 with Ris added the same amount of new bone to tibial diaphysis as did PGE2 alone and upon withdrawal, new marrow trabecular bone was lost but new periosteal and endocortical bones were preserved in PGE2 + 1 microgram of Ris on/off group. In contrast, all the new bone was maintained in the PGE2 + 5 micrograms of Ris on/off group; (4) PGE2 + Ris cotreatment failed to block the increase in cortical bone porosity induced by PGE2; and (5) in the PGE2 alone and PGE2 + 1 microgram of Ris on/off groups bone turnover was higher than that in the PGE2 + 5 micrograms of Ris on/off group. These results indicate that on/off treatment with PGE2 and Ris is superior to PGE2 alone in that it forms the same amount of new bone during treatment, but preserves more cortical bone during withdrawal. Depression of bone resorption and turnover were the tissue mechanisms responsible for this protection.

NASA Program Space Physiology and Countermeasures↗

A Note on the Relationship between Temperature and Water Vapor in Quasi-Equilibrium and Climate States

An ideal and simple formulation is successfully derived that well represents a quasi-linear relationship found between the domain-averaged water vapor, q (mm), and temperature, T (K), fields obtained from a series of quasi-equilibrium (long-term) simulations for the Tropics using the two-dimensional Goddard Cumulus Ensemble (GCE) model. Earlier model work showed that the forced maintenance of two different wind profiles in the Tropics leads to two different equilibrium states. Investigating this finding required investigation of the slope of the moisture-temperature relations, which turns out to be linear in the Tropics. The extra-tropical climate equilibriums become more complex, but insight on modeling sensitivity can be obtained by linear stepwise regression of the integrated temperature and humidity. A globally curvilinear moisture-temperature distribution, similar to the famous Clausius-Clapeyron curve (i.e., saturated water vapor pressure versus temperature), is then found in this study. Such a genuine finding clarifies that the dynamics are crucial to the climate (shown in the earlier work) but the thermodynamics adjust. The range of validity of this result is further examined herein. The GCE-modeled tropical domain-averaged q and T fields form a linearly-regressed "q-T" slope that genuinely resides within an ideal range of slopes obtained from the aforementioned formulation. A quantity (denoted as dC2/dC1) representing the derivative between the static energy densities due to temperature (C2) and water vapor (C1) for various quasi-equilibrium states can also be obtained. A dC2/dC1 value near unity obtained for the GCE-modeled tropical simulations implies that the static energy densities due to moisture and temperature only differ by a pure constant for various equilibrium states. An overall q-T relation also including extra-tropical regions is, however, found to have a curvilinear relationship. Accordingly, warm/moist regions favor change in water vapor faster than temperature, while cold/dry regions favor an increase in temperature quicker than water vapor.

Shie, C.-L.↗

Path perception during rotation: influence of instructions, depth range, and dot density

How do observers perceive their direction of self-motion when traveling on a straight path while their eyes are rotating? Our previous findings suggest that information from retinal flow and extra-retinal information about eye movements are each sufficient to solve this problem for both perception and active control of self-motion [Vision Res. 40 (2000) 3873; Psych. Sci. 13 (2002) 485]. In this paper, using displays depicting translation with simulated eye rotation, we investigated how task variables such as instructions, depth range, and dot density influenced the visual system's reliance on retinal vs. extra-retinal information for path perception during rotation. We found that path errors were small when observers expected to travel on a straight path or with neutral instructions, but errors increased markedly when observers expected to travel on a curved path. Increasing depth range or dot density did not improve path judgments. We conclude that the expectation of the shape of an upcoming path can influence the interpretation of the ambiguous retinal flow. A large depth range and dense motion parallax are not essential for accurate path perception during rotation, but reference objects and a large field of view appear to improve path judgments.

Space Perception/physiology↗

Innovative Robot Archetypes for In-Space Construction and Maintenance

The space environment presents unique challenges and opportunities in the assembly, inspection and maintenance of orbital and transit spaceflight systems. While conventional Extra-Vehicular Activity (EVA) technology, out of necessity, addresses each of the challenges, relatively few of the opportunities have been exploited due to crew safety and reliability considerations. Extra-Vehicular Robotics (EVR) is one of the least-explored design spaces but offers many exciting innovations transcending the crane-like Space Shuttle and International Space Station Remote Manipulator System (RMS) robots used for berthing, coarse positioning and stabilization. Microgravity environments can support new robotic archetypes with locomotion and manipulation capabilities analogous to undersea creatures. Such diversification could enable the next generation of space science platforms and vehicles that are too large and fragile to launch and deploy as self-contained payloads. Sinuous manipulators for minimally invasive inspection and repair in confined spaces, soft-stepping climbers with expansive leg reach envelopes and free-flying nanosatellite cameras can access EVA worksites generally not accessible to humans in spacesuits. These and other novel robotic archetypes are presented along with functionality concepts

Rehnmark, Fredrik↗

Centaur: A Mobile Dexterous Humanoid for Surface Operations

Future human and robotic planetary expeditions could benefit greatly from expanded Extra-Vehicular Activity (EVA) capabilities supporting a broad range of multiple, concurrent surface operations. Risky, expensive and complex, conventional EVAs are restricted in both duration and scope by consumables and available manpower, creating a resource management problem. A mobile, highly dexterous Extra-Vehicular Robotic (EVR) system called Centaur is proposed to cost-effectively augment human astronauts on surface excursions. The Centaur design combines a highly capable wheeled mobility platform with an anthropomorphic upper body mounted on a three degree-of-freedom waist. Able to use many ordinary handheld tools, the robot could conserve EVA hours by relieving humans of many routine inspection and maintenance chores and assisting them in more complex tasks, such as repairing other robots. As an astronaut surrogate, Centaur could take risks unacceptable to humans, respond more quickly to EVA emergencies and work much longer shifts. Though originally conceived as a system for planetary surface exploration, the Centaur concept could easily be adapted for terrestrial military applications such as de-Gig, surveillance and other hazardous duties.

Rehnmark, Fredrik↗

Drag Prediction for the DLR-F6 Wing/Body and DPW Wing using CFL3D and OVERFLOW Overset Mesh

A series of overset grids was generated in response to the 3rd AIAA CFD Drag Prediction Workshop (DPW-III) which preceded the 25th Applied Aerodynamics Conference in June 2006. DPW-III focused on accurate drag prediction for wing/body and wing-alone configurations. The grid series built for each configuration consists of a coarse, medium, fine, and extra-fine mesh. The medium mesh is first constructed using the current state of best practices for overset grid generation. The medium mesh is then coarsened and enhanced by applying a factor of 1.5 to each (I,J,K) dimension. The resulting set of parametrically equivalent grids increase in size by a factor of roughly 3.5 from one level to the next denser level. CFD simulations were performed on the overset grids using two different RANS flow solvers: CFL3D and OVERFLOW. The results were post-processed using Richardson extrapolation to approximate grid converged values of lift, drag, pitching moment, and angle-of-attack at the design condition. This technique appears to work well if the solution does not contain large regions of separated flow (similar to that seen n the DLR-F6 results) and appropriate grid densities are selected. The extra-fine grid data helped to establish asymptotic grid convergence for both the OVERFLOW FX2B wing/body results and the OVERFLOW DPW-W1/W2 wing-alone results. More CFL3D data is needed to establish grid convergence trends. The medium grid was utilized beyond the grid convergence study by running each configuration at several angles-of-attack so drag polars and lift/pitching moment curves could be evaluated. The alpha sweep results are used to compare data across configurations as well as across flow solvers. With the exception of the wing/body drag polar, the two codes compare well qualitatively showing consistent incremental trends and similar wing pressure comparisons.

Sclanfani, Anthony J.↗

The High Resolution Tropospheric Ozone Residual

The co-flight of the MLS stratospheric limb sounder and the Ozone Monitoring Instrument (OMI) provides the capability of computing the Tropospheric Ozone Residual (TOR) in much greater detail [Ziemke et al., 2006]. Using forward trajectory calculations of MLS ozone measurements combined with OMI column ozone we have developed a high horizontal resolution tropospheric ozone residual (HTOR) which can provide even more detail than the standard TOR product. HTOR is especially useful for extra-tropical studies of tropospheric ozone transport. We find that both the Pacific pollution corridor (East Asia to Alaska) and the Atlantic pollution corridor (North America east coast to Europe) are also preferred locations for strat-trop folds leading to systematic overestimates of pollution amounts. In fact, fold events appear to dominate extra-tropical Northern Hemisphere day-to-day maps of HTOR. Model estimates of the tropospheric column are in reasonable agreement with the HTOR amounts when offsets due to different tropopause height calculations are taken into consideration.

Schoeberl, Mark R.↗

Testing Special Relativity at High Energies with Astrophysical Sources

Since the group of Lorentz boosts is unbounded, there is a question as to whether Lorentz invariance (LI) holds to infinitely short distances. However, special and general relativity may break down at the Planck scale. Various quantum gravity scenarios such as loop quantum gravity, as well as some forms of string theory and extra dimension models may imply Lorentz violation (LV) at ultrahigh energies. The Gamma-Ray Large Area Space Telescope (GLAST), to be launched in mid-December, will measure the spectra of distant extragalactic sources of high energy gamma-rays, particularly active galactic nuclei and gamma-ray bursts. GLAST can look for energy-dependent gamma-ray propagation effects from such sources as a signal of Lorentz invariance violation. These sources may also exhibit the high energy cutoffs predicted to be the result of intergalactic annihilation interactions with low energy photons having a flux level as determined by various astronomical observations. With LV the threshold for such interactions can be significantly raised, changing the predicted absorption turnover in the observed spectrum of the sources. Stecker and Glashow have shown that the existence such absorption features in the spectra of extragalactic sources puts constraints on LV. Such constraints have important implications for some quantum gravity and large extra dimension models. Future spaceborne detectors dedicated to measuring gamma-ray polarization can look for birefringence effects as a possible signal of loop quantum gravity. A very small LV may also result in the modification or elimination of the GZK effect, thus modifying the spectrum of ultrahigh energy cosmic rays. This possibility can be explored with ground-based arrays such as Auger or with a space based detector system such as the proposed OWL satellite mission.

Stecker, F. W.↗

The Role of Non-Targeted Effects as Mediators in the Biological Effects of Proton Irradiation

In recent years, the hypothesis that non-DNA targets are primary initiators and mediators of the biological effects of ionizing radiation, such as proton beams and heavy ions, has gained much interest. These phenomena have been denoted as non-targeted or bystander effects to distinguish them from the more traditionally studied model that focuses on direct damage to DNA causing chromosomal rearrangements and mutations as causative of most biological endpoints such as cell killing, tissue damage, and cancer. We review cellular and extra-cellular structures and signal transduction pathways that have been implemented in these recent studies. Non-targeted effects of interest include oxidative damage to the cytoplasm and mitochondria, disruption of the extra-cellular matrix, and modification of cytokine signaling including TGF-beta, and gap junction communication. We present an introduction to these targets and pathways, and contrast there role with DNA damage pathways.

Cucinotta, Francis A.↗

Spatial and Temporal Inter-Relationship between Anomalies and Trends of Temperature, Moisture, Cloud Cover and OLR as Observed by AIRS/AMSU on Aqua

AIRS/AMSU is the advanced IR/MW atmospheric sounding system launched on EOS Aqua in May 2002. Products derived from AIRS/AMSU by the AIRS Science Team include surface skin temperature and atmospheric temperature profiled; atmospheric humidity profiles, fractional cloud clover and cloud top pressure, and OLR. Products covering the period September 2002 through the present have been derived from AIRS/AMSU using the AIRS Science Team Version 5 retrieval algorithm. In this paper, we will show results covering the time period September 2006 - November 2008. This time period is marked by a substantial warming trend of Northern Hemisphere Extra-tropical land surface skin temperatures, as well as pronounced El Nino - La Nina episodes. These both influence the spatial and temporal anomaly patterns of atmospheric temperature and moisture profiles, as well as of cloud cover and Clear Sky and All Sky OLR. The relationships between temporal and spatial anomalies of these parameters over this time period, as determined from AIRS/AMSU observations, will be shown with particular emphasis on which contribute significantly to OLR anomalies in each of the tropics and extra-tropics. Results will also be shown to evaluate the anomalies and trends of temperature profiles and OLR as determined from analysis of AIRS/AMSU data. Global and regional trends during the 6 1/3 year time period are not necessarily indicative of what has happened in the past, or what may happen in the future. Nevertheless, the inter-relationships of spatial and temporal anomalies of atmospheric geophysical parameters with those of surface skin temperature are indicative of climate processes, and can be used to test the performance of climate models when driven by changes in surface temperatures.

Susskind, Joel↗

Complementary Paired G4FETs as Voltage-Controlled NDR Device

It is possible to synthesize a voltage-controlled negative-differential-resistance (NDR) device or circuit by use of a pair of complementary G4FETs (four-gate field-effect transistors). [For more information about G4FETs, please see the immediately preceding article]. As shown in Figure 1, the present voltage-controlled NDR device or circuit is an updated version of a prior NDR device or circuit, known as a lambda diode, that contains a pair of complementary junction field-effect transistors (JFETs). (The lambda diode is so named because its current-versus- voltage plot bears some resemblance to an upper-case lambda.) The present version can be derived from the prior version by substituting G4FETs for the JFETs and connecting both JFET gates of each G4FET together. The front gate terminals of the G4FETs constitute additional terminals (that is, terminals not available in the older JFET version) to which one can apply control voltages VN and VP. Circuits in which NDR devices have been used include (1) Schmitt triggers and (2) oscillators containing inductance/ capacitance (LC) resonant circuits. Figure 2 depicts such circuits containing G4FET NDR devices like that of Figure 1. In the Schmitt trigger shown here, the G4FET NDR is loaded with an ordinary inversion-mode, p-channel, metal oxide/semiconductor field-effect transistor (inversion-mode PMOSFET), the VN terminal of the G4FET NDR device is used as an input terminal, and the input terminals of the PMOSFET and the G4FET NDR device are connected. VP can be used as an extra control voltage (that is, a control voltage not available in a typical prior Schmitt trigger) for adjusting the pinch-off voltage of the p-channel G4FET and thereby adjusting the trigger-voltage window. In the oscillator, a G4FET NDR device is loaded with a conventional LC tank circuit. As in other LC NDR oscillators, oscillation occurs because the NDR counteracts the resistance in the tank circuit. The advantage of this G4FET-NDR LC oscillator over a conventional LC NDR oscillator is that one can apply a time-varying signal to one of the extra control input terminals (VN or VP) to modulate the conductance of the NDR device and thereby amplitude-modulate the output signal.

Mojarradi, Mohammad↗

EVA 2010: Preparing for International Space Station EVA Operations Post-Space Shuttle Retirement

The expected retirement of the NASA Space Transportation System (also known as the Space Shuttle ) by 2011 will pose a significant challenge to Extra-Vehicular Activities (EVA) on-board the International Space Station (ISS). The EVA hardware currently used to assemble and maintain the ISS was designed assuming that it would be returned to Earth on the Space Shuttle for refurbishment, or if necessary for failure investigation. With the retirement of the Space Shuttle, a new concept of operations was developed to enable EVA hardware (Extra-vehicular Mobility Unit (EMU), Airlock Systems, EVA tools, and associated support hardware and consumables) to perform ISS EVAs until 2015, and possibly beyond to 2020. Shortly after the decision to retire the Space Shuttle was announced, the EVA 2010 Project was jointly initiated by NASA and the OneEVA contractor team. The challenges addressed were to extend the operating life and certification of EVA hardware, to secure the capability to launch EVA hardware safely on alternate launch vehicles, to protect for EMU hardware operability on-orbit, and to determine the source of high water purity to support recharge of PLSSs (no longer available via Shuttle). EVA 2010 Project includes the following tasks: the development of a launch fixture that would allow the EMU Portable Life Support System (PLSS) to be launched on-board alternate vehicles; extension of the EMU hardware maintenance interval from 3 years (current certification) to a minimum of 6 years (to extend to 2015); testing of recycled ISS Water Processor Assembly (WPA) water for use in the EMU cooling system in lieu of water resupplied by International Partner (IP) vehicles; development of techniques to remove & replace critical components in the PLSS on-orbit (not routine); extension of on-orbit certification of EVA tools; and development of an EVA hardware logistical plan to support the ISS without the Space Shuttle. Assumptions for the EVA 2010 Project included no more than 8 EVAs per year for ISS EVA operations in the Post-Shuttle environment and limited availability of cargo upmass on IP launch vehicles. From 2010 forward, EVA operations on-board the ISS without the Space Shuttle will be a paradigm shift in safely operating EVA hardware on orbit and the EVA 2010 effort was initiated to accommodate this significant change in EVA evolutionary history.

Chullen, Cinda↗

Post-Shuttle EVA Operations on ISS

The expected retirement of the NASA Space Transportation System (also known as the Space Shuttle ) by 2011 will pose a significant challenge to Extra-Vehicular Activities (EVA) on-board the International Space Station (ISS). The EVA hardware currently used to assemble and maintain the ISS was designed assuming that it would be returned to Earth on the Space Shuttle for refurbishment, or if necessary for failure investigation. With the retirement of the Space Shuttle, a new concept of operations was developed to enable EVA hardware (Extra-vehicular Mobility Unit (EMU), Airlock Systems, EVA tools, and associated support hardware and consumables) to perform ISS EVAs until 2015, and possibly beyond to 2020. Shortly after the decision to retire the Space Shuttle was announced, the EVA 2010 Project was jointly initiated by NASA and the One EVA contractor team. The challenges addressed were to extend the operating life and certification of EVA hardware, to secure the capability to launch EVA hardware safely on alternate launch vehicles, to protect for EMU hardware operability on-orbit, and to determine the source of high water purity to support recharge of PLSSs (no longer available via Shuttle). EVA 2010 Project includes the following tasks: the development of a launch fixture that would allow the EMU Portable Life Support System (PLSS) to be launched on-board alternate vehicles; extension of the EMU hardware maintenance interval from 3 years (current certification) to a minimum of 6 years (to extend to 2015); testing of recycled ISS Water Processor Assembly (WPA) water for use in the EMU cooling system in lieu of water resupplied by International Partner (IP) vehicles; development of techniques to remove & replace critical components in the PLSS on-orbit (not routine); extension of on-orbit certification of EVA tools; and development of an EVA hardware logistical plan to support the ISS without the Space Shuttle. Assumptions for the EVA 2010 Project included no more than 8 EVAs per year for ISS EVA operations in the Post-Shuttle environment and limited availability of cargo upmass on IP launch vehicles. From 2010 forward, EVA operations on-board the ISS without the Space Shuttle will be a paradigm shift in safely operating EVA hardware on orbit and the EVA 2010 effort was initiated to accommodate this significant change in EVA evolutionary history. 1

West, William↗

Dust Tolerant EVA-Compatible Connectors

The objectives of this project are to develop connectors (quick disconnects and umbilical systems) that can be repetitively and reliably mated and de-mated during Lunar surface extra-vehicular activities. These standardized interfaces will be required for structural integrity and commodities transfer between linked surface elements. QD's fittings are needed for EVA spacesuit Primary Life Support Systems as well as liquid cooled garment circulation and suit heat rejection. Umbilical electro-mechanical systems (connectors) are needed between discrete surface systems for transfer of air, power, fluid (water), and data must be capable of being operated by extra vehicular astronaut crew members and/or robotic assistants. There exists an urgent need to prevent electro-statically charged dust and debris from clogging and degrading the interface seals and causing leakage and spills of hazardous commodities, contaminating the flowstream, and degrading the mechanisms needed for umbilical connection. Other challenges include modularity, standardization, autonomous operation, and lifetime sealing issues.

Mueller, Robert P.↗

Complexity Analysis of Traffic in Corridors-in-the-Sky

The corridors-in-the-sky concept imitates the highway system in ground transportation. The benefit expected from a corridor relies on its capability of handling high density traffic with negligible controller workload, the acceptance of extra fuel or distance, and the complexity reduction in underlying sectors. This work evaluates a selected corridor from these perspectives through simulations. To examine traffic inside the corridor, a corridor traffic simulation tool that can resolve conflicts is developed using C language. Prescribed conflict resolution maneuvers mimic corridor users behaviors and conflict resolution counts measure complexity. Different lane options and operational policies are proposed to examine their impacts on complexity. Fuel consumption is calculated and compared for corridor traffic. On the other hand, to investigate the complexity of non-corridor traffic in underlying sectors, the existing Airspace Concept Evaluation System tool is utilized along with the Automated Airspace Concept tool. The number of conflict resolutions is examined and treated as the complexity measurement. The results show heavy traffic can be managed with low complexity for a historical traffic schedule simulated with appropriate operational policies and lane options. For instance, with 608 flights and peak aircraft count of 100, only 84 actions need to be taken in a 24-hour period to resolve the conflicts for an 8-lane corridor. Compared with the fuel consumptions with great circle trajectories, the simulation of corridor traffic shows that the total extra fuel for corridor flights is 26,373 gallons, or 2.76%, which is 0.38% less than flying filed flight plans. Without taking climb and descent portions of corridor traffic, the complexity of underlying sectors is reduced by 17.71%. However the climb and descent portions will eliminate the reduction and the overall complexity of sectors is actually increased by 9.14%.

Xue, Min↗