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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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All sourcesNASA NTRSDOE OSTI
NASA NTRS2004

Fluorescent Approaches to High Throughput Crystallography

X-ray crystallography remains the primary method for determining the structure of macromolecules. The first requirement is to have crystals, and obtaining them is often the rate-limiting step. The numbers of crystallization trials that are set up for any one protein for structural genomics, and the rate at which they are being set up, now overwhelm the ability for strictly human analysis of the results. Automated analysis methods are now being implemented with varying degrees of success, but these typically cannot reliably extract intermediate results. By covalently modifying a subpopulation, less than or = 1%, of a macromolecule solution with a fluorescent probe, the labeled material will add to a growing crystal as a microheterogeneous growth unit. Labeling procedures can be readily incorporated into the final stages of a macromolecules purification. The covalently attached probe will concentrate in the crystal relative to the solution, and under fluorescent illumination the crystals will show up as bright objects against a dark background. As crystalline packing is more dense than amorphous precipitate, the fluorescence intensity can be used as a guide in distinguishing different types of precipitated phases, even in the absence of obvious crystalline features, widening the available potential lead conditions in the absence of clear "bits." Non-protein structures, such as salt crystals, will not incorporate the probe and will not show up under fluorescent illumination. Also, brightly fluorescent crystals are readily found against less fluorescent precipitated phases, which under white light illumination may serve to obscure the crystals. Automated image analysis to find crystals should be greatly facilitated, without having to first define crystallization drop boundaries and by having the protein or protein structures all that show up. The trace fluorescently labeled crystals will also emit with sufficient intensity to aid in the automation of crystal alignment using relatively low cost optics, further increasing throughput at synchrotrons. This presentation will focus on the methodology for fluorescent labeling, the crystallization results, and the effects of the trace labeling on the crystal quality.

Minamitani, Elizabeth Forsythe↗
NASA NTRS2004

Fluorescent Approaches to High Throughput Crystallography

X-ray crystallography remains the primary method for determining the structure of macromolecules. The first requirement is to have crystals, and obtaining them is often the rate-limiting step. The numbers of crystallization trials that are set up for any one protein for structural genomics, and the rate at which they are being set up, now overwhelm the ability for strictly human analysis of the results. Automated analysis methods are now being implemented with varying degrees of success, but these typically can not reliably extract intermediate results. By covalently modifying a subpopulation, less than or = 1%, of a macromolecule solution with a fluorescent probe, the labeled material will add to a growing crystal as a microheterogeneous growth unit. Labeling procedures can be readily incorporated into the final stages of purification. The covalently attached probe will concentrate in the crystal relative to the solution, and under fluorescent illumination the crystals show up as bright objects against a dark background. As crystalline packing is more dense than amorphous precipitate, the fluorescence intensity can be used as a guide in distinguishing different types of precipitated phases, even in the absence of obvious crystalline features, widening the available potential lead conditions in the absence of clear "hits." Non-protein structures, such as salt crystals, will not incorporate the probe and will not show up under fluorescent illumination. Also, brightly fluorescent crystals are readily found against less fluorescent precipitated phases, which under white light illumination may serve to obscure the crystals. Automated image analysis to find crystals should be greatly facilitated, without having to first define crystallization drop boundaries and by having the protein or protein structures all that show up. The trace fluorescently labeled crystals will also emit with sufficient intensity to aid in the automation of crystal alignment using relatively low cost optics, further increasing throughput at synchrotrons. This presentation will focus on the methodology for fluorescent labeling, the crystallization results, and the effects of the trace labeling on the crystal quality.

Pusey, Marc L.↗
NASA NTRS2005

Fluorescent Approaches to High Throughput Crystallography

X-ray crystallography remains the primary method for determining the structure of macromolecules. The first requirement is to have crystals, and obtaining them is often the rate-limiting step. The numbers of crystallization trials that are set up for any one protein for structural genomics, and the rate at which they are being set up, now overwhelm the ability for strictly human analysis of the results. Automated analysis methods are now being implemented with varying degrees of success, but these typically cannot reliably extract intermediate results. By covalently modifying a subpopulation, 51%, of a macromolecule solution with a fluorescent probe, the labeled material will add to a growing crystal as a microheterogeneous growth unit. Labeling procedures can be readily incorporated into the final stages of purification. The covalently attached probe will concentrate in the crystal relative to the solution, and under fluorescent illumination the crystals show up as bright objects against a dark background. As crystalline packing is more dense than amorphous precipitate, the fluorescence intensity can be used as a guide in distinguishing different types of precipitated phases, even in the absence of obvious crystalline features, widening the available potential lead conditions in the absence of clear hits. Non-protein structures, such as salt crystals, will not incorporate the probe and will not show up under fluorescent illumination. Also, brightly fluorescent crystals are readily found against less fluorescent precipitated phases, which under white light illumination may serve to obscure the crystals. Automated image analysis to find crystals should be greatly facilitated, without having to first define crystallization drop boundaries and by having the protein or protein structures all that show up. The trace fluorescently labeled crystals will also emit with sufficient intensity to aid in the automation of crystal alignment using relatively low cost optics, further increasing throughput at synchrotrons. This presentation will focus on the methodology for fluorescent labeling, the crystallization results, and the effects of the trace labeling on the crystal quality.

Pusey, Marc L.↗
NASA NTRS2005

Fluorescent Approaches to High Throughput Crystallography

X-ray crystallography remains the primary method for determining the structure of macromolecules. The first requirement is to have crystals, and obtaining them is often the rate-limiting step. The numbers of crystallization trials that are set up for any one protein for structural genomics, and the rate at which they are being set up, now overwhelm the ability for strictly human analysis of the results. Automated analysis methods are now being implemented with varying degrees of success, but these typically cannot reliably extract intermediate results. By covalently modifying a subpopulation, less than or = 1 %, of a macromolecule solution with a fluorescent probe, the labeled material will add to a growing crystal as a microheterogeneous growth unit. Labeling procedures can be readily incorporated into the final stages of purification. The covalently attached probe will concentrate in the crystal relative to the solution, and under fluorescent illumination the crystals show up as bright objects against a dark background. As crystalline packing is more dense than amorphous precipitate, the fluorescence intensity can be used as a guide in distinguishing different types of precipitated phases, even in the absence of obvious crystalline features, widening the available potential lead conditions in the absence of clear "hits." Non-protein structures, such as salt crystals, will not incorporate the probe and will not show up under fluorescent illumination. Also, brightly fluorescent crystals are readily found against less fluorescent precipitated phases, which under white light illumination may serve to obscure the crystals. Automated image analysis to find crystals should be greatly facilitated, without having to first define crystallization drop boundaries and by having the protein or protein structures all that show up. The trace fluorescently labeled crystals will also emit with sufficient intensity to aid in the automation of crystal alignment using relatively low cost optics, further increasing throughput at synchrotrons. Preliminary experiments show that the presence of the fluorescent probe does not affect the nucleation process or the quality of the X-ray data obtained.

Pusey, Marc L.↗
NASA NTRS1990

X ray sensitive area detection device

A radiation sensitive area detection device is disclosed which comprises a phosphor-containing film capable of receiving and storing an image formed by a pattern of incoming x rays, UV, or other radiation falling on the film. The device is capable of fluorescing in response to stimulation by a light source in a manner directly proportional to the stored radiation pattern. The device includes: (1) a light source capable of projecting light or other appropriate electromagnetic wave on the film so as to cause it to fluoresce; (2) a means to focus the fluoresced light coming from the phosphor-containing film after light stimulation; and (3) at least one charged coupled detector or other detecting element capable of receiving and digitizing the pattern of fluoresced light coming from the phosphor-containing film. The device will be able to generate superior x ray images of high resolution from a crystal or other sample and will be particularly advantageous in that instantaneous near-real-time images of rapidly deteriorating samples can be obtained. Furthermore, the device can be made compact and sturdy, thus capable of carrying out x ray or other radiation imaging under a variety of conditions, including those experienced in space.

Carter, Daniel C.↗
NASA NTRS2012

Filling-in of Near-infrared Solar Lines by Terrestrial Fluorescence and Other Geophysical Effects: Simulations and Space-based Observations from SCIAMACHY and GOSAT

Global mapping of terrestrial vegetation fluorescence from space has recently been accomplished with high spectral resolution (nu/nu greater than 35 000) measurements from the Japanese Greenhouse gases Observing SAellite (GOSAT). These data are of interest because they can potentially provide global information on the functional status of vegetation including light-use efficiency and global primary productivity that can be used for global carbon cycle modeling. Quantifying the impact of fluorescence on the O2-A band is important as this band is used for photon pathlength characterization in cloud- and aerosol-contaminated pixels for trace-gas retrievals including CO2. Here, we examine whether fluorescence information can be derived from space using potentially lower-cost hyperspectral instrumentation, i.e., more than an order of magnitude less spectral resolution (nu/nu approximately 1600) than GOSAT, with a relatively simple algorithm. We discuss laboratory measurements of fluorescence near one of the few wide and deep solar Fraunhofer lines in the long-wave tail of the fluorescence emission region, the calcium (Ca) II line at 866 nm that is observable with a spectral resolution of approximately 0.5 nm. The filling-in of the Ca II line due to additive signals from various atmospheric and terrestrial effects, including fluorescence, is simulated. We then examine filling-in of this line using the SCanning Imaging Absorption spectroMeter for Atmospheric CHartographY (SCIAMACHY) satellite instrument. In order to interpret the satellite measurements, we developed a general approach to correct for various instrumental artifacts that produce false filling-in of solar lines in satellite measurements. The approach is applied to SCIAMACHY at the 866 nm Ca II line and to GOSAT at 758 and 770 nm on the shoulders of the O2-A feature where there are several strong solar Fraunhofer lines that are filled in primarily by vegetation fluorescence. Finally, we compare temporal and spatial variations of SCIAMACHY additive signals with those of GOSAT and the Enhanced Vegetation Index (EVI) from the MODerate-resolution Imaging Spectroradiometer (MODIS). Although the derived additive signals from SCIAMACHY are extremely weak at 866 nm, their spatial and temporal variations are consistent with chlorophyll a fluorescence or another vegetation-related source. We also show that fillingin occurs at 866 nm over some barren areas, possibly originating from luminescent minerals in rock and soil.

vegetation↗
NASA NTRS2012

Spectroscopic Chemical Analysis Methods and Apparatus

This invention relates to non-contact spectroscopic methods and apparatus for performing chemical analysis and the ideal wavelengths and sources needed for this analysis. It employs deep ultraviolet (200- to 300-nm spectral range) electron-beam-pumped wide bandgap semiconductor lasers, incoherent wide bandgap semiconductor lightemitting devices, and hollow cathode metal ion lasers. Three achieved goals for this innovation are to reduce the size (under 20 L), reduce the weight [under 100 lb (.45 kg)], and reduce the power consumption (under 100 W). This method can be used in microscope or macroscope to provide measurement of Raman and/or native fluorescence emission spectra either by point-by-point measurement, or by global imaging of emissions within specific ultraviolet spectral bands. In other embodiments, the method can be used in analytical instruments such as capillary electrophoresis, capillary electro-chromatography, high-performance liquid chromatography, flow cytometry, and related instruments for detection and identification of unknown analytes using a combination of native fluorescence and/or Raman spectroscopic methods. This design provides an electron-beampumped semiconductor radiation-producing method, or source, that can emit at a wavelength (or wavelengths) below 300 nm, e.g. in the deep ultraviolet between about 200 and 300 nm, and more preferably less than 260 nm. In some variations, the method is to produce incoherent radiation, while in other implementations it produces laser radiation. In some variations, this object is achieved by using an AlGaN emission medium, while in other implementations a diamond emission medium may be used. This instrument irradiates a sample with deep UV radiation, and then uses an improved filter for separating wavelengths to be detected. This provides a multi-stage analysis of the sample. To avoid the difficulties related to producing deep UV semiconductor sources, a pumping approach has been developed that uses ballistic electron beam injection directly into the active region of a wide bandgap semiconductor material.

Hug, William F.↗
NASA NTRSDate not supplied

Creating Benchmark Data for Artificial Intelligence and Machine Learning Space Biology Research

To identify an appropriate AI/ML approach for a specific problem, the best practice is to measure algorithm performance through the benchmarking process. A scientific benchmark consists of an AI-ready dataset and a reference implementation on a specific scientific question. The NASA Science Mission Directorate (SMD) has started the “Benchmark Initiative for AI/ML to create scientific benchmark datasets in three applications: 1) scientific benchmarking, which finds the best algorithm for a specific problem; 2) application benchmarking, which measures algorithm performance against a set of parameters; and 3) system benchmarking, which evaluates performance of hardware and software architecture. Currently, there are no standardized datasets available to benchmark AI/ML algorithms in the domain of space biology. In this work, we constructed two AI/ML-ready biological datasets from experiments in space-flown mice: cellular imaging and RNA-seq. First, radiation-exposed immune cells harbor DNA damage foci that can be fluorescently marked to visualize the amount of damage following exposure to ionizing radiation. However, such large datasets are difficult to analyze visually, due to imaging inconsistencies and human bias, and classical image processing approaches can fail on imaging artifacts. AI/ML are therefore exciting alternative, providing the speed of machines and the accuracy of humans. We have made this dataset available at https://registry.opendata.aws/bps_microscopy/. Second, high-throughput nucleic acid sequencing (DNA-seq, RNA-seq) has become widespread in biomedical research due to the growing availability and affordability of these assays. However, most sequencing datasets suffer from high dimensionality and low sample count. In this work, we used a generative adversarial network to synthesize a standardized, AI-ready, publicly available benchmark dataset for space biology RNA-seq data with sufficient space-flown and ground control mouse liver samples from NASA GeneLab. This dataset is available at https://registry.opendata.aws/bps_rnaseq/. These datasets are now fully open the Space Biology community to test their favorite AI/ML approaches.

James Casaletto↗
NASA NTRS2010

High-Speed PLIF Imaging of Hypersonic Transition over Discrete Cylindrical Roughness

In two separate test entries, advanced laser-based instrumentation has been developed and applied to visualize the hypersonic flow over cylindrical protrusions on a flat plate. Upstream of these trips, trace quantities of nitric oxide (NO) were seeded into the boundary layer. The protuberances were sized to force laminar-to-turbulent boundary layer transition. In the first test, a 10-Hz nitric oxide planar laser-induced fluorescence (NO PLIF) flow visualization system was used to provide wide-field-of-view, high-resolution images of the flowfield. The images had sub-microsecond time resolution. However these images, obtained with a time separation of 0.1 sec, were uncorrelated with each other. Fluorescent oil-flow visualizations were also obtained during this test. In the second experiment, a laser and camera system capable of acquiring NO PLIF measurements at 1 million frames per second (1 MHz) was used. This system had lower spatial resolution, and a smaller field of view, but the images were time correlated so that the development of the flow structures could be observed in time.

Danehy, P. M.↗
NASA NTRS1993

Serendipitous images of methanol in Comet Levy (1900 XX)

Reuter's (1992) model of the IR fluorescence of methanol is used to retrieve a methanol production rate of 3 +/- 1 x 10 exp 26/s and an abundance relative to water of about 0.1 percent. It is argued that calibration is of paramount importance and that a near-simultaneous spectrum is necessary for achieving a reliable estimate of the continuum underlying the emission feature.

Hoban, Susan↗
NASA NTRS2002

Molecular Probe Fluorescence Monitoring of Polymerization

This project investigated the feasibility of using fluorescence spectroscopy to determine viscosity of polymer/monomer in support of Transient Interfacial Phenomena in Miscible Polymer Systems (TIPMPS). This project will attempt to measure gradient induced flow at a miscible interface during and / or after in-flight polymerization of dodecyl acrylate (lauryl acrylate). Concentration and temperature gradients will be intentionally introduced during polymerization and the resultant fluid flow determined by Particle Imaging Velocimetry (PIV). This report describes an investigation of the feasibility of using fluorescence of a probe molecule to monitor viscosity and/or concentration during and after polymerization. The probe used was pyrene which has been shown to be sensitive to its local environment in methyl methacrylate.

Patrick Bunton↗
NASA NTRS1977

Lunar surface chemistry - A new imaging technique

Detailed chemical maps of the lunar surface have been constructed by applying a new weighted-filter imaging technique to Apollo 15 and Apollo 16 X-ray fluorescence data. The data quality improvement is amply demonstrated by (1) modes in the frequency distribution, representing highland and mare soil suites, which are not evident before data filtering, and (2) numerous examples of chemical variations which are correlated with small-scale (about 15 kilometer) lunar topographic features.

Andre, C. G.↗
NASA NTRS1992

Exit plane H2O concentration measurements correlated with OH PLIF near-injector mixing measurements for scramjet flows

Mixing and combusting high enthalpy flows, similar to those encountered in scramjet engines, were investigated using a shock tunnel to produce the flow in conjunction with non-intrusive optical diagnostics which monitored the performance of two injector configurations. The shock tunnel is configured to produce Mach 3 flow and stagnation enthalpies corresponding to flight equivalent Mach numbers between 7 and 11. A pulsed hydrogen injection capability and interchangeable injector blocks provide a means of examining high speed, high enthalpy reacting flows. Planar laser induced fluorescence (PLIF) of OH molecules in the near injector region produced images which show the combusting and mixing zones for the reacting flow. Line-of-sight exit plane measurement of water concentration and temperature were used to provide a unique method of monitoring exit plane products. These results demonstrated that a velocity matched axial injection system produced a fuel jet that lifted off the floor of the duct. Mixing was observed to increase for this system as a velocity mismatch was introduced. Comparison of exit plane water concentrations for a wall jet injection system and a velocity matched injection system indicated similar mixing performance but an accurate pressure measurement is necessary to further validate the result. In addition, exit plane measurements indicated an approximate steady-state condition was achieved during the 1 to 2 ms test times.

Parker, T. E.↗
NASA NTRS1995

Thermospheric nitric oxide from the ATLAS 1 and Spacelab 1 missions

Spectral and spatial images obtained with the Imaging Spectrometric Observatory on the ATLAS 1 and Spacelab 1 missions are used to study the ultraviolet emissions of nitric oxide in the thermosphere. By synthetically fitting the measured NO gamma bands, intensities are derived as a function of altitude and latitude. We find that the NO concentrations inferred from the ATLAS 1 measurements are higher than predicted by our thermospheric airglow model and tend to lie to the high side of a number of earlier measurements. By comparison with synthetic spectral fits, the shape of the NO gamma bands is used to derive temperature as a function of altitude. Using the simultaneous spectral and spatial imaging capability of the instrument, we present the first simultaneously acquired altitude images of NO gamma band temperature and intensity in the thermosphere. The lower thermospheric temperature images show structure as a function of altitude. The spatial imaging technique appears to be a viable means of obtaining temperatures in the middle and lower thermosphere, provided that good information is also obtained at the higher altitudes, as the contribution of the overlying, hotter NO is nonnegligible. By fitting both self-absorbed and nonabsorbed bands of the NO gamma system, we show that the self absorption effects are observable up to 200 km, although small above 150 km. The spectral resolution of the instrument (1.6 A) allows separation of the N(+)(S-5) doublet, and we show the contribution of this feature to the combination of the NO gamma (1, 0) band and the N(+)(S-5) doublet as a function of altitude (less than 10% below 200 km). Spectral images including the NO delta bands support previous findings that the fluorescence efficiency is much higher than that determined from laboratory measurements. The Spacelab 1 data indicate the presence of a significant population of hot NO in the vehicle environment of that early shuttle mission.

Torr, Marsha R.↗
NASA NTRS2005

CHAMP - Camera, Handlens, and Microscope Probe

CHAMP (Camera, Handlens And Microscope Probe) is a novel field microscope capable of color imaging with continuously variable spatial resolution from infinity imaging down to diffraction-limited microscopy (3 micron/pixel). As an arm-mounted imager, CHAMP supports stereo-imaging with variable baselines, can continuously image targets at an increasing magnification during an arm approach, can provide precision range-finding estimates to targets, and can accommodate microscopic imaging of rough surfaces through a image filtering process called z-stacking. Currently designed with a filter wheel with 4 different filters, so that color and black and white images can be obtained over the entire Field-of-View, future designs will increase the number of filter positions to include 8 different filters. Finally, CHAMP incorporates controlled white and UV illumination so that images can be obtained regardless of sun position, and any potential fluorescent species can be identified so the most astrobiologically interesting samples can be identified.

Mungas, G. S.↗
NASA NTRS2007

Characterization of Three Carbon- and Nitrogen-Rich Particles from Comet 81P/WILD

Comets may sample the early solar system s complement of volatile-forming elements - including C and N - more fully and reliably than do the terrestrial planets or asteroids. Until recently, all elemental analyses of unambiguously cometary material were carried out remotely. The return of the Stardust mission makes it possible to analyze documented material from P81/Wild 2 in the laboratory Wild 2 particles fragmented when they stopped in the aerogel collectors. We have studied three fragments thought to be rich in C and N by using several techniques: FTIR to characterize organic matter; synchrotron-induced x-ray fluorescence (SXRF) to determine Fe and certain element/Fe ratios; SEM to image sample morphology and to detect semiquantitatively Mg, Al, Si, Ca, and Fe; and nuclear reaction analysis (NRA) to measure C, N, O, and Si.

Gallien, J.-P.↗
NASA NTRS2012

Investigations of a Combustor Using a 9-Point Swirl-Venturi Fuel Injector: Recent Experimental Results

This paper explores recent results obtained during testing in an optically-accessible, JP8-fueled, flame tube combustor using baseline Lean Direct Injection (LDI) research hardware. The baseline LDI geometry has nine fuel/air mixers arranged in a 3 x 3 array. Results from this nine-element array include images of fuel and OH speciation via Planar Laser-Induced Fluorescence (PLIF), which describe fuel spray pattern and reaction zones. Preliminary combustion temperatures derived from Stokes/Anti-Stokes Spontaneous Raman Spectroscopy are also presented. Other results using chemiluminescence from major combustion radicals such as CH* and C2* serve to identify the primary reaction zone, while OH PLIF shows the extent of reaction further downstream. Air and fuel velocities and fuel drop size results are also reported.

Hicks, Yolanda R.↗
NASA NTRS1992

Performance of a multistep fluorescence-gated proportional counter for hard X-ray astronomy

Results from the first flight of our proportional counter in an imaging telescope led us to rebuild the detector. We have used a Penning gas mixture (xenon + 1 percent isobutylene) and introduced a preamplification region to improve the energy resolution. We have rebuilt the pressure vessel making novel use of molybdenum as the housing material in order to reduce the residual instrument background, particularly in the fluorescence-gated mode for which the detector design has been optimized. We have also increased the sensitive gas depth from 9 to 14 cm to further increase the sensitivity to both fluorescent pairs and conventional singles. Our calibrations have shown that the overall energy resolution of the detector has been enhanced by a factor of 2, and we predict that the sensitivity at float will increase by a factor of 3 in the 50-70 keV energy band.

Dietz, K. L.↗