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At least 271 records · Page 15

K in an Urban World: New Contexts for Hydraulic Conductivity

Abstract Hydraulic conductivity ( K ) is a key hydrologic parameter widely recognized to be difficult to estimate and constrain, with little consistent assessment in disturbed, urbanized soils. To estimate K , it is either measured, or simulated by pedotransfer functions, which relate K to easily measured soil properties. We measured K in urbanized soils by double‐ring infiltrometer ( K dring ), near‐saturated tension infiltrometry ( K minidisk ), and constant head borehole permeametry ( K borehole ), along with other soil properties across the major soil orders in 12 United States cities. We compared measured K with that predicted from the pedotransfer function, ROSETTA. We found that regardless of soil texture, K dring was consistently larger than K minidisk ; with the latter having slightly less sample variance. K borehole was dependent upon specific subsurface conditions, and contrary to common expectations, did not always decrease with depth. Based on either soil textural class, or percent textural separates (sand, silt clay), ROSETTA did not accurately predict measured K for surface nor subsurface soils. We go on to discuss how K varies in urban landscapes, the role of measurement methods and artifacts in the perception of this metric, and implications for hydrologic modeling. Overall, we aim to inspire consistency and coherence when addressing K ‐related challenges in sustainable urban water management.

Shuster, W. D.↗

Neurobehavioral symptoms in children born extremely preterm: A Swedish National Study

Abstract Aim To determine the prevalence of neurobehavioral symptoms at 6.5 years in children born extremely preterm (EPT, <27 weeks' gestation). Methods Population‐based cohort study of infants born EPT in Sweden from 2004 to 2007. Of 486 survivors 375 were assessed and compared with 369 matched term‐born controls. EPT children free from neurosensory and intellectual disabilities (neurodevelopmental disabilities [NDD]‐free, n = 236) were compared separately. Standardised questionnaires were used to assess parental ratings of hyperactivity and attention, emotional, peer‐relation, conduct and social problems; and deficits in perception, language and memory. Results EPT children had more reported problems in all assessed neurobehavioral domains than controls, with more than three times greater odds for most outcomes. Except for conduct problems, increased problems were identified also in NDD–free children. The odds of having neurobehavioral problems in ≥3 co‐occurring domains were five (whole EPT group) and three (NDD‐free group) times higher than in controls. Conclusion EPT children with or without NDD have more neurobehavioral problems in multiple domains than term peers. Ongoing assessments of behaviour until school age or beyond should recognise early symptoms of attention, everyday social problems, perceptual, emotional or language difficulties.

Serenius, Fredrik↗

The Connected We St@nd programme: A feasibility pilot study of an online self‐management intervention for adults on in‐centre haemodialysis and family caregivers

Abstract Objective The ‘Connected We St@nd’ is an online self‐management intervention programme for people receiving in‐centre haemodialysis and family caregivers that combines an educational and psychosocial support component. This study aimed to evaluate its feasibility and acceptability before proceeding to a large‐scale trial. Design This was a pre‐post single‐arm feasibility pilot study conducted with adults undergoing in‐centre haemodialysis and family caregivers. Methods Feasibility was based on eligibility, consent, retention, completion and intervention adherence rates, while acceptability was assessed in post‐intervention focus group interviews. Results Twenty‐six people (16 adults on haemodialysis and 10 family caregivers) recruited through social networks completed the intervention. Consent, retention and completion rates were excellent (>90%) and eligibility (77.5%) and intervention adherence were satisfactory (69% for the psychosocial support sessions). Qualitative findings revealed that participants shared positive feelings regarding their participation in the programme. The valuable interactions with group peers and health psychologists during the support sessions, the perception of the adequacy and coherence of the programme's contents and materials and the participants' confidence in using the platform developed to deliver the intervention were some of the aspects highlighted as facilitators of intervention acceptability. Additionally, people on haemodialysis and caregivers reported that participation in the programme brought several educational and emotional benefits (e.g., additional disease‐related knowledge, improved communication and coping skills, greater confidence in managing dialysis complications or caregiving demands) that helped increase their self‐management skills and psychosocial adjustment to the demands of kidney failure and renal therapies. Conclusions The results suggested that the ‘Connected We St@nd’ programme is likely to be feasible and acceptable for adults on haemodialysis and family caregivers, thus representing a promising resource for the future of interdisciplinary renal rehabilitation. Suggestions were made to fine‐tune the intervention design to proceed with a large‐scale trial.

Sousa, Helena↗

Promoting Physical Activity During Pregnancy and the Postpartum Period

Introduction Physical activity is important for improving and maintaining overall health across the life span, including during and after pregnancy. Achieving recommended levels of physical activity can be challenging during pregnancy and the postpartum period. The US Office of Disease Prevention and Health Promotion sought to promote physical activity during and after pregnancy through the development of health education materials for the Move Your Way campaign. Research was conducted with pregnant and postpartum people to learn what types of messages and materials would encourage physical activity in these populations. Methods Participants were recruited from 3 regions of the United States to participate in 90‐minute virtual focus groups. Eligible participants were aged 18 years or older and either pregnant or 6 weeks to 1 year postpartum. Participants were asked questions about their beliefs, attitudes, and perceptions about physical activity and prompted to provide feedback on health promotion messages and images. Sessions were recorded, transcribed, and analyzed for key themes. Results Twenty‐four focus groups were conducted with 48 pregnant participants and 52 postpartum participants. Sixteen sessions were conducted in English and 8 were conducted in Spanish. Most participants had questions about how much physical activity is recommended, and many cited their health care provider as a trusted source of information. Participants responded positively to materials that acknowledged the uniqueness of each pregnant or postpartum experience, referenced gradually increasing physical activity levels, highlighted the benefits of physical activity, focused on safety, addressed common barriers, and displayed realistic representations of physical activity. Discussion There is an opportunity to improve messaging about physical activity during and after pregnancy. To better promote physical activity, perinatal health care providers and other health professionals can share information about recommended amounts of physical activity, communicate the benefits, and promote realistic and achievable physical activity messages that address common barriers in these populations.

Nursing↗

A multisensory approach to understanding bat responses to wind energy developments

Abstract Millions of bats are killed at wind energy facilities worldwide, yet the behavioural mechanisms underlying why bats are vulnerable to wind turbines remain unclear. Anthropogenic stimuli that alter perceptions of the environment, known as sensory pollution, could create ecological traps and cause bat mortality at wind farms. We review the sensory abilities of bats to evaluate potential stimuli associated with wind farms and examine the role of spatial scale on the perceptual mechanisms of sensory pollutants associated with wind energy facilities. Audition, vision, somatosensation and olfaction are sensory modalities that bats use to perceive their environment, including wind farms and turbine structures, but they will not all be useful at the same spatial scales. Bats most likely use vision to perceive wind farms on the landscape, and obstruction lighting may be the first sensory cue to attract bats to wind farms from kilometres away. Research that assesses the risks posed by specific sensory pollutants, when conducted at the appropriate scale, can help identify solutions to reduce bat mortality, such as determining the attractiveness of obstruction lighting to bats at a landscape scale.

Jonasson, Kristin A.↗

Xylella fastidiosa causes transcriptional shifts that precede tylose formation and starch depletion in xylem

Abstract Pierce's disease (PD) in grapevine ( Vitis vinifera ) is caused by the bacterial pathogen Xylella fastidiosa . X. fastidiosa is limited to the xylem tissue and following infection induces extensive plant‐derived xylem blockages, primarily in the form of tyloses. Tylose‐mediated vessel occlusions are a hallmark of PD, particularly in susceptible V. vinifera . We temporally monitored tylose development over the course of the disease to link symptom severity to the level of tylose occlusion and the presence/absence of the bacterial pathogen at fine‐scale resolution. The majority of vessels containing tyloses were devoid of bacterial cells, indicating that direct, localized perception of X. fastidiosa was not a primary cause of tylose formation. In addition, we used X‐ray computed microtomography and machine‐learning to determine that X. fastidiosa induces significant starch depletion in xylem ray parenchyma cells. This suggests that a signalling mechanism emanating from the vessels colonized by bacteria enables a systemic response to X. fastidiosa infection. To understand the transcriptional changes underlying these phenotypes, we integrated global transcriptomics into the phenotypes we tracked over the disease spectrum. Differential gene expression analysis revealed that considerable transcriptomic reprogramming occurred during early PD before symptom appearance. Specifically, we determined that many genes associated with tylose formation (ethylene signalling and cell wall biogenesis) and drought stress were up‐regulated during both Phase I and Phase II of PD. On the contrary, several genes related to photosynthesis and carbon fixation were down‐regulated during both phases. These responses correlate with significant starch depletion observed in ray cells and tylose synthesis in vessels.

59 BASIC BIOLOGICAL SCIENCES↗

Constitutive and inducible oleoresin defenses share genetic architectures and mechanisms in Pinus taeda

The oleoresin defense system of loblolly pine (Pinus taeda) protects trees from insects and pathogens and is an important source of renewable biofuels and chemicals, but the genetic basis of oleoresin production is poorly understood. We characterized the genetic architecture of oleoresin flow, resin canal number, stem wood terpene content, and monoterpene composition in two clonal populations of P. taeda. We used quantitative genetic analyses, genome-wide association studies (GWASs), multiplex network learning, and gene expression profiling to elucidate shared gene networks underlying defense traits and to identify high-quality candidates for breeding and engineering loblolly pine. Genetic analyses revealed polygenic inheritance and trait-to-trait correlations provide strong evidence for shared genes regulating constitutive and induced oleoresin flow. We identified 236 single nucleotide polymorphisms associated with oleoresin flow, resin canal number, and terpene composition and highlight candidate genes likely involved in terpene biosynthesis, cambial meristem reprogramming, and pathogen perception and immune signaling. Fourteen GWAS candidates were methyl jasmonate-responsive in tissues where resin canals initiate and terpene production occurs. Integrating quantitative genetics, GWAS, gene expression, and multiplex network analyses enabled the prioritization of high-quality candidate genes. This work advances the development of more resilient loblolly pine optimized for ecological performance, renewable chemical, and biofuel production.

genome-wide association study↗

Ecological information and approaches needed for risk communication dialogs for acute or chronic environmental crises

Scientists, social scientists, risk communicators, and many others are often thrust into a crisis situation where they need to interact with a range of stakeholders, including governmental personnel (tribal, U.S. federal, state, local), local residents, and other publics, as well as other scientists and other risk communicators in situations where information is incomplete and evolving. This paper provides: (1) an overall framework for thinking about communication during crises, from acute to chronic, and local to widespread, (2) a template for the types of ecological information needed to address public and environmental concerns, and (3) examples to illustrate how this information will aid risk communicators. The main goal is providing an approach to the knowledge needed by communicators to address the challenges of protecting ecological resources during an environmental crisis, or for an on-going, chronic environmental issue. To understand the risk to these ecological resources, it is important to identify the type of event, whether it is acute or chronic (or some combination of these), what receptors are at risk, and what stressors are involved (natural, biological, chemical, radiological). For ecological resources, the key information a communicator needs for a crisis is whether any of the following are present: threatened or endangered species, species of special concern, species groups of concern (e.g., neotropical bird migrants, breeding frogs in vernal ponds, rare plant assemblages), unique or rare habitats, species of commercial and recreational interest, and species/habitats of especial interest for medicinal, cultural, or religious activities. Communication among stakeholders is complicated with respect to risk to ecological receptors because of differences in trust, credibility, empathy, perceptions, world view valuation of the resources, and in many cases, a history of misinformation, disinformation, or no information. Exposure of salmon spawning in the Columbia River to hexavalent chromium from the Hanford Site is used as an example of communication challenges with different stakeholders, including Native Americans with Tribal Treaty rights to the land.

54 ENVIRONMENTAL SCIENCES↗

Plant terpene specialized metabolism: complex networks or simple linear pathways?

From the perspectives of pathway evolution, discovery and engineering of plant specialized metabolism, the nature of the biosynthetic routes represents a critical aspect. Classical models depict biosynthesis typically from an end-point angle and as linear, for example, connecting central and specialized metabolism. As the number of functionally elucidated routes increased, the enzymatic foundation of complex plant chemistries became increasingly well understood. The perception of linear pathway models has been severely challenged. With a focus on plant terpenoid specialized metabolism, we review here illustrative examples supporting that plants have evolved complex networks driving chemical diversification. The completion of several diterpene, sesquiterpene and monoterpene routes shows complex formation of scaffolds and their subsequent functionalization. These networks show that branch points, including multiple sub-routes, mean that metabolic grids are the rule rather than the exception. This concept presents significant implications for biotechnological production.

60 APPLIED LIFE SCIENCES↗

Ectomycorrhizal symbiosis prepares its host locally and systemically for abiotic cue signaling

Tree growth and survival are dependent on their ability to perceive signals, integrate them, and trigger timely and fitted molecular and growth responses. While ectomycorrhizal symbiosis is a predominant tree-microbe interaction in forest ecosystems, little is known about how and to what extent it helps trees cope with environmental changes. We hypothesized that the presence of Laccaria bicolor influences abiotic cue perception by Populus trichocarpa and the ensuing signaling cascade. We submitted ectomycorrhizal or non-ectomycorrhizal P. trichocarpa cuttings to short-term cessation of watering or ozone fumigation to focus on signaling networks before the onset of any physiological damage. Poplar gene expression, metabolite levels, and hormone levels were measured in several organs (roots, leaves, mycorrhizas) and integrated into networks. We discriminated the signal responses modified or maintained by ectomycorrhization. Ectomycorrhizas buffered hormonal changes in response to short-term environmental variations systemically prepared the root system for further fungal colonization and alleviated part of the root abscisic acid (ABA) signaling. Furthermore, the presence of ectomycorrhizas in the roots also modified the leaf multi-omics landscape and ozone responses, most likely through rewiring of the molecular drivers of photosynthesis and the calcium signaling pathway. In conclusion, P. trichocarpa-L. bicolor symbiosis results in a systemic remodeling of the host's signaling networks in response to abiotic changes. In addition, ectomycorrhizal, hormonal, metabolic, and transcriptomic blueprints are maintained in response to abiotic cues, suggesting that ectomycorrhizas are less responsive than non-mycorrhizal roots to abiotic challenges.

60 APPLIED LIFE SCIENCES↗

Thinking Beyond the Default User: The Impact of Gender, Stereotypes, and Modality on Interpretation of User Needs

Throughout the mechanical design process, designers, the majority of whom are men, often fail to consider the needs of women, resulting in consequences ranging from inconvenience to increased risk of serious injury or death. Although these biases are well studied in other fields of research, the mechanical design field lacks formal investigation into this phenomenon. In this study, engineering students (n = 301) took a survey in which they read a Persona describing a student makerspace user and a Walkthrough describing the user’s interaction with the makerspace while completing a project. During the Walkthrough, the user encountered various obstacles or Pain Points. Participants were asked to recall and evaluate the Pain Points that the user encountered and then evaluated their perceptions of the makerspace and user. Furthermore, the independent variables under investigation were the gender of the user Persona (woman, gender-neutral, or man), the Walkthrough room case (crafting or woodworking makerspace), and the modality of the Persona and Walkthrough (text- or audio-based). Results showed that participants from the Text-based modality were better able to recall Pain Points compared to participants from the Audio-based modality. Pain Points were assessed as more severe when they impacted women users, potentially stemming from protective paternalism. In addition to finding that the gender of a user impacted the way a task environment was perceived, results confirmed the presence of androcentrism, or “default man” assumptions, in the way designers view end users of unknown gender. Promisingly, providing user Persona information in an audio modality significantly reduced this bias compared to text-based modalities, indicating that providing richer detail in user personas has the capability to reduce gender bias in designers.

cognitive-based design↗

Planned Relocation: A Pluralistic and Integrated Approach to Science and Governance

Planned relocation is an ambitious objective that occurs in complex governance settings rife with inequalities of power, discrimination, distrust, deep uncertainty, misalignment of incentives and authorities, and other challenges. Taking planned relocation from ambition to an equitable and effective portfolio of measures will require changes in science, governance and how they work together. Research will need to understand perverse incentives, conflicts, limits to risk perception, and other factors across individuals, communities, governance levels, and sectors. Knowledge to enable planned relocation will emerge not just from technical studies but from entwined processes of action, learning and capacity building. Science and governance must advance their collaboration to better support and learn from these tangled processes to identify adaptive pathways, build agency and implement strategies under deep uncertainty.

global environmental change, adapatation, decision↗

Argonaute and Dicer are essential for communication between Trichoderma atroviride and fungal hosts during mycoparasitism

ABSTRACT Trichoderma species are known for their mycoparasitic activity against phytopathogenic fungi that cause significant economic losses in agriculture. During mycoparasitism, Trichoderma spp. recognize molecules produced by the host fungus and release secondary metabolites and hydrolytic enzymes to kill and degrade the host’s cell wall. Here, we explored the participation of the Trichoderma atroviride RNAi machinery in the interaction with six phytopathogenic fungi of economic importance. We determined that both Argonaute-3 and Dicer-2 play an essential role during mycoparasitism. Using an RNA-Seq approach, we identified that perception, detox, and cell wall degradation depend on the T. atroviride -RNAi when interacting with Alternaria alternata, Rhizoctonia solani AG2 , and R. solani AG5. Furthermore, we constructed a gene co-expression network that provides evidence of two gene modules regulated by RNAi, which play crucial roles in essential processes during mycoparasitism. In addition, based on small RNA-seq, we conclude that siRNAs regulate amino acid and carbon metabolism and communication during the Trichoderma -host interaction. Interestingly, our data suggest that siRNAs might regulate allorecognition ( het ) and transport genes in a cross-species manner. Thus, these results reveal a fine-tuned regulation in T. atroviride dependent on siRNAs that is essential during the biocontrol of phytopathogenic fungi, showing a greater complexity of this process than previously established. IMPORTANCE There is an increasing need for plant disease control without chemical pesticides to avoid environmental pollution and resistance, and the health risks associated with the application of pesticides are increasing. Employing Trichoderma species in agriculture to control fungal diseases is an alternative plant protection strategy that overcomes these issues without utilizing chemical fungicides. Therefore, understanding the biocontrol mechanisms used by Trichoderma species to antagonize other fungi is critical. Although there has been extensive research about the mechanisms involved in the mycoparasitic capability of Trichoderma species, there are still unsolved questions related to how Trichoderma regulates recognition, attack, and defense mechanisms during interaction with a fungal host. In this work, we report that the Argonaute and Dicer components of the RNAi machinery and the small RNAs they process are essential for gene regulation during mycoparasitism by Trichoderma atroviride .

59 BASIC BIOLOGICAL SCIENCES↗

Scalable Bayesian Physics-Informed Kolmogorov-Arnold Networks

Uncertainty quantification (UQ) plays a pivotal role in scientific machine learning, especially when surrogate models are used to approximate complex systems. Although multilayer perceptions (MLPs) are commonly employed as surrogates, they often suffer from overfitting due to their large number of parameters. Kolmogorov-Arnold networks (KANs) offer an alternative solution with fewer parameters. However, gradient-based inference methods, such as Hamiltonian Monte Carlo (HMC), may result in computational inefficiency when applied to KANs, especially for large-scale datasets, due to the high cost of back-propagation. To address these challenges, we propose a novel approach, combining the dropout Tikhonov ensemble Kalman inversion (DTEKI) with Chebyshev KANs. This gradient-free method effectively mitigates overfitting and enhances numerical stability. In addition, we incorporate the active subspace method to reduce the parameter-space dimensionality, allowing us to improve the accuracy of predictions and obtain more reliable uncertainty estimates. Extensive experiments demonstrate the efficacy of our approach in various test cases, including scenarios with large datasets and high noise levels. Our results show that the new method achieves comparable or better accuracy, much higher efficiency as well as stability compared to HMC, in addition to scalability. Moreover, by leveraging the low-dimensional parameter subspace, our method preserves prediction accuracy while substantially reducing further the computational cost.

97 MATHEMATICS AND COMPUTING↗

From carbon to climate action: harnessing basalt for geological carbon dioxide (CO 2 ) storage onshore and offshore

Abating emissions and reducing atmospheric concentrations of carbon dioxide (CO 2 ) are crucial in avoiding catastrophic climate change. Basalt reservoirs offer suitable CO 2 storage sites and are present onshore and offshore. This study compares the geological, economic and social aspects of CO 2 storage in both domains using two basalt formations from the Republic of Ireland: the Limerick Igneous Suite and the Druid Formation. Offshore reservoirs offer larger storage resources but are more expensive to develop. Nevertheless, both offer gigatonne-scale theoretical CO 2 storage. Reviewing public perception studies on wind energy demonstrates that the commonly held belief that offshore projects face less opposition owing to their distance from communities has been dispelled, while similar studies on carbon capture and removal show no preference for onshore or offshore development. Instead, a general scepticism towards carbon removal is observed, with concerns that it hinders broader decarbonization efforts. The desire of all stakeholders to be involved throughout project development is consistently observed. Lessons from offshore natural gas projects highlight the consequences of failing to engage with local communities. The Republic of Ireland has significant potential to deploy geological CO 2 storage in basalt reservoirs, but policy hurdles such as legal limits on storage volumes must be overcome to reconcile climate ambition and legislative capacity.

54 - ENVIRONMENTAL SCIENCES/GLOBAL CLIMATE CHANGE ↗

Enhancing trap and fault seal analyses by integrating observations from HR3D seismic data with well logs and conventional 3D seismic data, Texas inner shelf

An understanding of trap and fault seal quality is critical for assessing hydrocarbon prospectivity. To achieve this, modern analytical techniques leverage well data and conventional industry-standard 3D seismic data to evaluate the trap, and any faults displacing the reservoir and top seal intervals. Above all, geological interpretation provides the framework of trap and fault seal analyses, but can be hindered by the data resolution, quality and acquisition style of the conventional seismic data. Furthermore, limiting the analysis to only the petroleum system at depth may lead to erroneous perceptions because interpreting overburden features, such as shallow faults or gas chimneys, can provide valuable observations with respect to container performance, and can to help validate trap and fault seal predictions. Here, a supplement to conventional 3D data are high-resolution 3D seismic (HR3D) data, which provide detailed images of the overburden geology. This study utilizes an HR3D seismic volume in the San Luis Pass area of the Texas inner shelf, where shallow fault tips and a sizeable gas chimney are interpreted over an unsuccessful hydrocarbon prospect. Static post-drill fault seal and trap analyses suggest that the primary fault displacing the structural closure could have withheld columns of gas c. 100 m high, but disagree with our HR3D seismic interpretations and dry-well analyses. From our results, we hypothesize that tertiary gas migration through fault conduits reduced the hydrocarbon column in the prospective Early Miocene reservoir, and may have resulted from continued movement along the intersecting faults. Overall, this study reinforces the importance of understanding the overburden geology and geohistory of faulted prospects, and demonstrates the utility of pre-drill HR3D acquisition when conducting trap and fault seal analyses.

3D seismic↗

Hypergames and Cyber-Physical Security for Control Systems

The identification of the Stuxnet worm in 2010 provided a highly publicized example of a cyber attack that physically damaged an industrial control system. This raised public awareness about the possibility of similar attacks against other industrial targets—including critical infrastructure. Here, we use hypergames to analyze how strategic perturbations of sensor readings and calibrated parameters can be used to manipulate a system that employs optimal control. Hypergames form an extension of game theory that enables us to model strategic interactions where the players may have significantly different perceptions of the game(s) they are playing. Past work with hypergames has focused on relatively simple interactions consisting of a small set of discrete choices for each player. Here, we apply single-stage hypergames to larger systems with continuous variables. We find that manipulating constraints can be a more effective attacker strategy than manipulating objective function parameters. Moreover, the attacker need not change the underlying system to carry out a successful attack—it may be sufficient to deceive the defender controlling the system. It is possible to scale our approach up to even larger systems, but this will depend on the characteristics of the system in question, and we identify several characteristics that will make those systems amenable to hypergame analysis.

97 MATHEMATICS AND COMPUTING↗