Results for “stacking sequences” — Page 15

NASA NTRS1989

An algorithm for computing the distance spectrum of trellis codes

A class of quasiregular codes is defined for which the distance spectrum can be calculated from the codeword corresponding to the all-zero information sequence. Convolutional codes and regular codes are both quasiregular, as well as most of the best known trellis codes. An algorithm to compute the distance spectrum of linear, regular, and quasiregular trellis codes is presented. In particular, it can calculate the weight spectrum of convolutional (linear trellis) codes and the distance spectrum of most of the best known trellis codes. The codes do not have to be linear or regular, and the signals do not have to be used with equal probabilities. The algorithm is derived from a bidirectional stack algorithm, although it could also be based on the Viterbi algorithm. The algorithm is used to calculate the beginning of the distance spectrum of some of the best known trellis codes and to compute tight estimates on the first-event-error probability and on the bit-error probability.

Rouanne, Marc↗

NASA NTRS1995

Characterization of Two-Dimensionally Braided Composites Subject to Static and Fatigue Loading

This paper presents a characterization of damage processes in four different two-dimensionally braided architectures consisting of graphite fibers in an epoxy matrix, subjected to both static and fatigue loading. Both notched and unnotched specimens were tested from each architecture. Damage mechanisms in static compression testing were found to range from matrix cracking in an architecture controlled by shearing characteristics of the matrix to axial fiber bundle kinking and splitting in architectures controlled by unidirectional fiber bundles. In both static tension and tension-tension fatigue testing, it was found that all architectures sustained the same basic damage types and progression sequences. The first types of damage to occur were splits in the braider bundles and cracks in the resin rich areas. Once a sufficient density of this type of damage had occurred, the axial bundles began to disbond from the surrounding constituents, and continued to do so until ultimate failure. One architecture sustained an additional mode of failure, which involved splitting of the axial bundles along their length. Varied degrees of notch sensitivity were found among the different architectures. In one architecture, this was attributed to differences found in the stacking of the individual plys of the braided architecture. As the density of axial bundles adjacent to the notch increased, the rate of stiffness loss increased and the life decreased.

Burr, Scott T.↗

NASA NTRS1973

Lidar Observations of Raman Scattering from S02 in a Power Plant Stack Plume

LIDAR techniques have been successfully applied to the detection of the Raman backscatter from S02 in the plume of a 200 megawatt coal-burning electrical- generating plant from a distance of 210 meters. The LIDAR system used consists of a 61-centimeter-diameter, f /4 Newtonian telescope and a 1.0-1.5-joules per pulse, 1-pulse-per-second ruby laser. Narrow band interference filters are used to select the 7546-angstrom v (sub 1) vibrational line of S02. The signal from a photomultiplier tube was sequentially applied to each 254-nanosecond-wide channel of a 15-cihannel photon counting system, resulting in a direct correlation between channel number and range increment. Photon counts were accumulated from the backscatter of a number of laser pulses (typically 50 or 100), and the accumulated counts per channel printed on paper tape. One sequence of measurements was made during a two-hour period while the plant electrical output was being reduced by approximately 50 percent. Although the Raman system had not been quantitatively calibrated, the LIDAR data correlated well with the varying plant electrical output. N2 scattering observations were also made and an approximate quantitative S02 concentration obtained by ratioing the S02 data to N2 data. This ratio compared well to the in-situ measurements made during the same period by Environmental Protection Agency sampling instruments.

Brumfield, M. L.↗

NASA NTRS1999

Microgravity Experiments to Evaluate Electrostatic Forces in Controlling Cohesion and Adhesion of Granular Materials

The bulk behavior of dispersed, fluidized, or undispersed stationary granular systems cannot be fully understood in terms of adhesive/cohesive properties without understanding the role of electrostatic forces acting at the level of the grains themselves. When grains adhere to a surface, or come in contact with one another in a stationary bulk mass, it is difficult to measure the forces acting on the grains, and the forces themselves that induced the cohesion and adhesion are changed. Even if a single gain were to be scrutinized in the laboratory, it might be difficult, perhaps impossible, to define the distribution and character of surface charging and the three- dimensional relationship that charges (electrons, holes) have to one another. The hypothesis that we propose to test in microgravity (for dielectric materials) is that adhesion and cohesion of granular matter are mediated primarily by dipole forces that do not require the presence of a net charge; in fact, nominally electrically neutral materials should express adhesive and cohesive behavior when the neutrality results from a balance of positive and negative charge carriers. Moreover, the use of net charge alone as a measure of the electrical nature of grain-to-grain relationships within a granular mass may be misleading. We believe that the dipole forces arise from the presence of randomly-distributed positive and negative fixed charge carriers on grains that give rise to a resultant dipole moment. These dipole forces have long-range attraction. Random charges are created whenever there is triboelectrical activity of a granular mass, that is, whenever the grains experience contact/separation sequences or friction. Electrostatic forces are generally under-estimated for their role in causing agglomeration of dispersed grains in particulate clouds, or their role in affecting the internal frictional relationships in packed granular masses. We believe that electrostatic, in particular dipole-mediated processes, are pervasive and probably affect, at some level, everything from astrophysical-scale granular systems such as interstellar nebulae, protoplanetary dust and debris disks, planetary-scale systems such as debris palls from meteorite impact, volcanic eruptions, and aeolian dust storms, all the way to industrial-scale systems in mining, powder and grain processing, pharmaceuticals, and smoke-stack technologies. NASA must concern itself with the electrostatic behavior of dust and sand on Mars because of its potentially critical importance to human exploration. The motion and adhesion of martian surface materials will affect the design and performance of spacesuits, habitats, processing plants, solar panels, and any externally exposed equipment such as surface rovers or communication and weather stations. Additionally, the adhesion of dust and sand could greatly enhance contact with the potentially toxic components of the martian soil.

Marshall, J.↗

NASA NTRS2009

Ares I First Stage Booster Deceleration System: An Overview

In 2005, the Congressional NASA Authorization Act enacted a new space exploration program, the "Vision for Space Exploratien". The Constellation Program was formed to oversee the implementation of this new mission. With an intent not simply to support the International Space Station, but to build a permanent outpost on the Moon and then travel on to explore ever more distant terrains, the Constellation Program is supervising the development of a brand new fleet of launch vehicles, the Ares. The Ares lineup will include two new launch vehicles: the Ares I Crew Launch Vehicle and the Ares V Cargo Launch Vehicle. A crew exploration vehicle, Orion, will be launched on the Ares I. It will be capable of docking with the Space Station, the lunar lander, Altair, and the Earth Departure Stage of Ares V. The Ares V will be capable of lifting both large-scale hardware and the Altair into space. The Ares First Stage Team is tasked with developing the propulsion system necessary to liftoff from the Earth and loft the entire Ares vehicle stack toward low Earth orbit. The Ares I First Stage booster is a 12-foot diameter, five-segment, reusable solid rocket booster derived from the Space Shuttle's four segment reusable solid rocket booster (SRB). It is separated from the Upper Stage through the use of a Deceleration Subsystem (DSS). Booster Tumble Motors are used to induce the pitch tumble following separation from the Upper Stage. The spent Ares I booster must be recoverable using a parachute deceleration system similar to that of the Shuttle SRB heritage system. Since Ares I is much heavier and reenters the Earth's atmosphere from a higher altitude at a much higher velocity than the SRB, all of the parachutes must be redesigned to reliably meet the operational requisites of the new launch vehicles. This paper presents an overview of this new booster deceleration system. It includes comprehensive detail of the parachute deceleration system, its design and deployment sequences, including how and why it is being developed, the requirements it must meet, and the testing involved in its implementation.

King, Ron↗

NASA NTRS2008

Geologic Mapping of the Meridiani Region, Mars

The light toned bedrock that has been observed at the Mars Exploration Rover Opportunity landing site is an upper layer in a sequence >600 m thick in places. These outcrops contain mineral and textural signatures that require interaction of, and possibly formation from, water. Many distinct layers are visible in the remote sensing data (e.g. Figure 1) and no work has ever characterized the full set of these materials that cover an area >3 105 km2 spanning 20 of longitude. Thus, whatever water-related process( es?) altered, and possibly formed, the rocks at the Opportunity landing site extended over a vast region of Mars. Yet many questions remain to be answered, such as: (1) in what capacity did water form and alter the deposits?, (2) what are the temporal and spatial relations with other major events known from ancient Mars?, and (3) would this type of environment have been conducive to the development of life? To address these questions we are completing a detailed geologic, stratigraphic, and thermophysical properties study of this widespread terrain. Specifically, we are drafting a 1:2M-scale geological map covering the full extent of these water-related deposits. In tandem with the mapping, Hynek and Phillips [1] have conducted a preliminary stratigraphic analysis of the stack of materials. After mapping is complete, we will study the thermophysical properties of the varied layers to derive possible compositional information of the materials. These tasks serve several purposes including gaining an understanding of the complex nature of these materials, their potential source region(s), and their timing of emplacement. All of these efforts are necessary to place the observations by the Opportunity Rover in a broader context and prepare for potential future landed missions to the region. Understanding the large-scale paleohydrology of Mars is central to NASA s goals and vital for determining if life ever arose on the planet.

Hynek, B. M.↗

NASA NTRSDate not supplied

Parametric Studies of Human Mars Entry, Descent, and Landing Systems

This paper will present a parametric analysis for entry, descent, and landing (EDL) concepts, enabling rapid systems assessment and tradespace exploration. The entry system uses a hypersonic inflatable aerodynamic decelerator (HIAD) technology. The baseline system includes elements for Mars aerocapture (AC) and EDL segments of the mission. The mission concept of operations (ConOps) begins at Mars arrival in a polar inclination. After performing an aerocapture (AC) maneuver into a pre-defined Mars parking orbit, the AC HIAD is jettisoned. The rest of thevehicle stays in the parking orbit for up to one year. The EDL sequence starts with a deorbit burn at the apoapsis of the parking orbit. After hypersonic entry with the EDL HIAD, the entry system uses a supersonic retropropulsion maneuver to slow the vehicle for the descent and landing segments of the mission. The vehicle will maintain a constant velocity of 2.5 m/s for 5 seconds prior to landing.The system includes a Mars Ascent Vehicle (MAV), Mars Descent Module (MDM), and two HIADs. The MDM includes a primary structure, tanks, engines, and radiators. The primary structure is an aluminum-lithium (Al-Li)cruciform design similar to the structural design of the Apollo Lunar Module. The cruciform planform layout results in four outer bays, with adequate volume in the corners between outer bays to package four landing gears. The central bay is reserved for packaging the MAV and the recessed MAV engines. Two of the outer bays accommodate main propellant tanks, with one LOX and one CH4 tank in each bay. The two remaining outer bays each house four rocket engine systems and associated support structure. Each HIAD comprises an inflatable structure, flexible thermal protection system, gas, and gas generators. The HIAD design used in this study is a stacked-toroid concept with pairing loop straps and radial/chevron straps. The baseline system lands a 20-t payload on the Mars surface. It is assumed the vehicle arrives at 6.2 km/s relative velocity at 90° inclination and is captured to a 1-Sol parking orbit.The baseline design includes many assumptions such as margins, arrival state, ConOps options, parking orbit, physical dimensions, propellant options, and technology concepts. The impact of these parameters are quantified through systems-level sensitivity analyses, which capture the global impact—not at a component level—but at the systems level. The systems-level sensitivities expose major design drivers and importance of each assumption for a design.Through tradespace exploration, a wide range of systems parameters are examined and compared for several feasible design options. Studies have been completed for the following input parameters: payload mass, propellant options, AC/EDL ballistic coefficient, lander thrust to weight ratio (T/W) (surrogate for the maximum EDL g’s),engine specific impulse (Isp), parking orbit, and inclination.The final paper will present and discuss the parametric approach used in the study. It will also include the results of recent systems analyses, sensitivity analyses, and tradespace exploration

Jamshid Samareh↗

NASA NTRS2019

The HST Lightcurve of (486958) 2014 MU69

We report Hubble Space Telescope (HST) lightcurve observations of the New Horizons spacecraft encounter Kuiper Belt object (KBO) (486958) 2014 MU69 acquired near opposition in July 2017. In order to plan the optimum flyby sequence the New Horizons mission planners needed to learn as much as possible about the target in advance of the encounter. Specifically, from lightcurve data, encounter timing could be adjusted to accommodate a highly elongated, binary, or rapidly rotating target. HST astrometric (Porter et al., 2018) and stellar occultation (Buie et al., 2018) observations constrained MU69's orbit and diameter (21–41 km for an albedo of 0.15–0.04), respectively. Photometry from the astrometric dataset suggested a variability of ≥0.3 mags, but they did not determine the period or provide shape information. To that end we strategically spaced 24 HST orbits over 9 days to investigate rotation periods from approximately 2–100 h and to better constrain the lightcurve amplitude. Until New Horizons detected MU69 in its optical navigation images beginning in August 2018, this HST lightcurve campaign provided the most accurate photometry to date. The mean variation in our data is 0.15 magnitudes which suggests that MU69 is either nearly spherical (a:b axis ratio of 1:1.15), or its pole vector is pointed near the line of sight to Earth; this interpretation does not preclude a near-contact binary or bi-lobed object. However, image stacks do conclude that MU69 does not have a binary companion ≥2000 km with a sensitivity to 29th magnitude (an object a few km in size for an albedo of 0.04–0.15). Our data are not of sufficient signal to noise to uniquely determine the period or amplitude, however, they did provide the necessary information for spacecraft planning. We report with confidence that MU69 is not both rapidly rotating and highly elongated (which we define as a lightcurve amplitude ≥ 0.5 magnitude). Since this paper is being published post fly-by, we note that our results are consistent with the fly-by imagery and orientation of MU69 (Stern et al., 2019). The combined dataset also suggests that within the KBO lightcurve literature there are likely other objects which share a geometric configuration like MU69 resulting in an underestimate of the contact binary fraction for the Cold Classical Kuiper Belt.

S D Benecchi↗

NASA NTRS2011

NASA Tech Briefs, January 2011

The topics include: 1) Distributed Aerodynamic Sensing and Processing Toolbox; 2) Collaborative Supervised Learning for Sensor Networks; 3) Hazard Detection Software for Lunar Landing; 4) Onboard Nonlinear Engine Sensor and Component Fault Diagnosis and Isolation Scheme; 5) Network-Capable Application Process and Wireless Intelligent Sensors for ISHM; 6) Interface Supports Multiple Broadcast Transceivers for Flight Applications; 7) FPGA Sequencer for Radar Altimeter Applications; 8) Miniature Sapphire Acoustic Resonator - MSAR; 9) Process-Hardened, Multi-Analyte Sensor for Characterizing Rocket Plume Constituents; 10) SAD5 Stereo Correlation Line-Striping in an FPGA; 11) Hybrid Composite Cryogenic Tank Structure; 12) Nanoscale Deformable Optics; 13) Reliability-Based Design Optimization of a Composite Airframe Component; 14) Zinc Oxide Nanowire Interphase for Enhanced Lightweight Polymer Fiber Composites; 15) Plasma Igniter for Reliable Ignition of Combustion in Rocket Engines; 16) Wire Test Grip Fixture; 17) A Sub-Hertz, Low-Frequency Vibration Isolation Platform; 18) Carbon Nanofibers Synthesized on Selective Substrates for Nonvolatile Memory and 3D Electronics; 19) Nanoparticle/Polymer Nanocomposite Bond Coat or Coating; 20) High-Resolution Wind Measurements for Offshore Wind Energy Development; 21) Spring Tire; 22) Marsviewer 2008; 23) Mission Services Evolution Center Message Bus; 24) Major Constituents Analysis for the Vehicle Cabin Atmosphere Monitor; 25) Astronaut Health Participant Summary Application; 26) Adaption of the AMDIS Method to Flight Status on the VCAM Instrument; 27) Natural Language Interface for Safety Certification of Safety-Critical Software; 28) Cryogenic Caging for Science Instrumentation; 29) Wide-Range Neutron Detector for Space Nuclear Applications; 30) In Situ Guided Wave Structural Health Monitoring System; 31) Multiplexed Energy Coupler for Rotating Equipment; 32) Attitude Estimation in Fractionated Spacecraft Cluster Systems; 33) Full Piezoelectric Multilayer-Stacked Hybrid Actuation/Transduction Systems; 34) Active Flow Effectors for Noise and Separation Control; 35) Method and System for Temporal Filtering in Video Compression Systems; 36) Apparatus for Measuring Total Emissivity of Small, Low-Emissivity Samples; 37) Multiple-Zone Diffractive Optic Element for Laser Ranging Applications; 38) Simplified Architecture for Precise Aiming of a Deep-Space Communication Laser Transceiver; 39) Two-Photon-Absorption Scheme for Optical Beam Tracking; 40) High-Sensitivity, Broad-Range Vacuum Gauge Using Nanotubes for Micromachined Cavities; 41) Wide-Field Optic for Autonomous Acquisition of Laser Link; 42) Extracting Zero-Gravity Surface Figure of a Mirror; 43) Modeling Electromagnetic Scattering From Complex Inhomogeneous Objects; 44) Visual Object Recognition and Tracking of Tools; 45) Method for Implementing Optical Phase Adjustment; 46) Visual SLAM Using Variance Grid Maps; 47) Rapid Calculation of Spacecraft Trajectories Using Efficient Taylor Series Integration; 48) Efficient Kriging Algorithms; 49) Predicting Spacecraft Trajectories by the WeavEncke Method; 50) An Augmentation of G-Guidance Algorithms; 51) Comparison of Aircraft Icing Growth Assessment Software; 52) Silicon-Germanium Voltage-Controlled Oscillator at 105 GHz; 53) Estimation of Coriolis Force and Torque Acting on Ares-1; 54) Null Lens Assembly for X-Ray Mirror Segments; and 55) High-Precision Pulse Generator.

Source record↗

NASA NTRS2023

Diverse Organic-Mineral Associations in Jezero Crater, Mars

The presence and distribution of preserved organic matter on the surface of Mars can provide key information about the Martian carbon cycle and the potential of the planet to host life throughout its history. Several types of organic molecules have been previously detected in Martian meteorites1 and at Gale crater, Mars. Evaluating the diversity and detectability of organic matter elsewhere on Mars is important for understanding the extent and diversity of Martian surface processes and the potential availability of carbon sources1,5,6. Here we report the detection of Raman and fluorescence spectra consistent with several species of aromatic organic molecules in the Máaz and Séítah formations within the Crater Floor sequences of Jezero crater, Mars. We report specific fluorescence-mineral associations consistent with many classes of organic molecules occurring in different spatial patterns within these compositionally distinct formations, potentially indicating different fates of carbon across environments. Our findings suggest there may be a diversity of aromatic molecules prevalent on the Martian surface, and these materials persist despite exposure to surface conditions. These potential organic molecules are largely found within minerals linked to aqueous processes, indicating that these processes may have had a key role in organic synthesis, transport or preservation.

Sunanda Sharma↗

NASA NTRSDate not supplied

Probabalistic Risk Analysis and Thermal Margin Process for an Inflatable Aeroshell

Uncertainties always exist in atmospheric entry aeroheating environments and the thermal response of thermal protection system (TPS) material. These uncertainties are mitigated in the design by ap-plying margin and factors of safety to the TPS. Entry vehicle TPS is often conservatively over-sized for the heat loads that are experienced along the entry trajectory by stacking worst-case scenarios together. Additionally, the current TPS design and margin process used by NASA offers very little insight into the risk of over-temperature during flight and the reliability of the heat shield performance [1,3]. A probabilistic margin process can be used to calculate the amount of TPS margin necessary to survive a given entry heat load at a specified level of risk [2,3,4]. The vehicle’s initial entry state (entry velocity, flight path angle, and entry mass) determines the expected atmospheric entry environmental conditions and resulting heat load that the entry vehicle will experience. If there is flexibility in the entry state, then this process can be used to select an appropriate combination of entry state parameters and TPS size to target a desired reentry reliability. This probabilistic margin process allows engineers to make informed aeroshell design, entry-trajectory design, and TPS performance risk trades while preventing excessive TPS margin from being applied. The probabilistic TPS margin process has been performed to determine TPS thickness and entry heating constraints given an acceptable risk level for the Low Earth Orbit Flight Experiment of an Inflatable Decelerator (LOFTID) flight project. The process is used in a manner to size the entry heat load for a given flexible TPS (FTPS) thickness so that it meets project reliability standards while allowing the FTPS and the underlying inflatable structure (IS) to be pushed to adequately high temperatures. Since the LOFTID project is an experimental flight demonstration, it is de-sired to drive the FTPS and IS to temperatures that cover a large range of their thermal response models’ applicability. This will allow the thermal response models to be better improved and validated post-flight using LOFTID’s extensive instrumentation embedded within the aeroshell. The presentation demonstrates how uncertainty analysis is carried out using an end-to-end Monte Carlo process where three separate Monte Carlo simulations are run in sequence. The first Monte Carlo simulation operates on the entry trajectory model to generate trajectory parameter dispersions that are fed into the second Monte Carlo simulation. The second Monte Carlo simulation operates on the aerothermodynamics model to generate aeroheating parameter dispersions that are fed into the third Monte Carlo simulation. The third Monte Carlo simulation operates on the FTPS material thermal response model to generate the final FTPS/IS thermal response dispersions. The end-to-end Monte Carlo simulation propagates the uncertainties of each model into the next to quantify the resulting uncertainty of the FTPS/IS thermal response. The fractional contributions of the uncertain parameters in the trajectory, aerothermal, and thermal response models to the variance in the FTPS/IS thermal response is determined as a byproduct of the Monte Carlo analysis. The structural uncertainty of the FTPS thermal response model is evaluated by flight relevant ground testing and model error analysis using test measurements. This probabilistic TPS margin process had never been applied to an entry vehicle and it is one of the LOFTID project’s goals to demonstrate its merits.

Steven A. Tobin↗

NASA NTRS2024

ISS External Microorganisms: A Planetary Protection Experiment to Inform Requirements for Crewed Missions to Mars

We have developed, tested, and flown a caddy capable of collecting aseptic samples from external surfaces of the ISS (International Space Station). The sampling caddy is certified for use during US EVA (extra vehicular activity) and was launched to ISS in the summer of 2023. We are scheduled to collect samples from 6 locations outside ISS during an EVA in May of 2024. We will freeze these samples at -80°C on orbit and return them to Earth. We will then extract and sequence any DNA collected during the EVA using next generation sequencing technologies to characterize the community composition and function of each sample. Measuring the type and quantity of microbes present on the exterior of ISS will allow us to address knowledge gap 2B, “Acceptable levels of microbial/organic releases from humans and support systems” described in a 2019 COSPAR report. Collecting data about microbial release from current crewed vehicles will inform requirements for acceptable leak rates for future crewed missions to Mars. The sampling kit consists of eight commercially available, sterile, DNA free, macrofoam swabs ( 23 mm. diameter ) installed in custom aluminum end effectors. Each end effector is housed in and individual aluminum canister. Each canister contains a 0.2 μm Teflon filter to allow the interior volume to accommodate pressure changes without permitting microbial contaminants to enter the sterile interior volume. A handle repurposed from the space shuttle tile repair kit is used to remove the end effector from the sample canister, collect a sample by swabbing a surface and then replace the end effector in its canister. The canisters and end effectors were cleaned and assembled on Earth. Prior to installing the sterile swab the canisters and end effectors were sterilized in an autoclave at 134°C, 215 kPA, for 7 min.. The final assembly occurred in a sterilized class II biosafety cabinet. We will collect six samples from the 1) airlock vestibule, 2) airlock thermal cover, 3) a gap in the micrometeorite shielding near the airlock, 4) a handrail near the airlock, 5) the CDRA (Carbon Dioxide Removal Assembly) vent, and 6) the VES (Vacuum Exhaust System) vent. The remaining two swabs will be reserved as controls. One swab will be exposed during the EVA without touching any surfaces to act as a blank. The final swab will remain sealed until the entire sampling kit is returned to earth. Based on previously published results from the Russian segment, we hypothesize that there will be detectable microbes at some or all of these locations. Ground-based testing of this sampling caddy confirms that the swabs remain sterile as the canisters transition in and out of vacuum. We were able to retrieve, viable bacterial and fungal cells as well as DNA from samples collected from US space suits during vacuum chamber tests lasting as long as seven hours. Based on these results and feedback from the test subjects the sampling caddy was modified to improve ergonomics and meet US EVA safety requirements. Bayonet probes were added to the sides of the sample kit as alternate mounting points. Additional locking features were added to the end effector and the filter stack to prevent inadvertent release during use. The opening mechanism was changed from one where the end effector was rocked laterally to defeat a ball detent to a twist-to-open threaded closure for similar reasons. Demonstrating, this sampling caddy’s effectiveness during a US EVA will allow us to address knowledge gaps identified in COSPAR reports and begin to define planetary protection requirements for life support systems on crewed missions to mars. This kit could also be used to collect contamination control samples during Artemis missions to verify requirements and could be easily modified for robotic sample collection.

Planetary Protection↗

NASA NTRSDate not supplied

ISS External Microorganisms: Collecting Planetary Protection Samples During Extravehicular Activity

We have developed, tested, and flown a caddy capable of collecting aseptic samples from external surfaces of the ISS (International Space Station). The sampling caddy is certified for use during US EVA (extra vehicular activity) and was launched to ISS in the summer of 2023. We are scheduled to collect samples from 6 locations outside ISS during an EVA in May of 2024. We will freeze these samples at -80°C on orbit and return them to Earth. We will then extract and sequence any DNA collected during the EVA using next generation sequencing technologies to characterize the community composition and function of each sample. Measuring the type and quantity of microbes present on the exterior of ISS will allow us to address knowledge gap 2B, “Acceptable levels of microbial/organic releases from humans and support systems” described in a 2019 COSPAR report. Collecting data about microbial release from current crewed vehicles will inform requirements for acceptable leak rates for future crewed missions to Mars. The sampling kit consists of eight commercially available, sterile, DNA free, macrofoam swabs ( 23 mm. diameter ) installed in custom aluminum end effectors. Each end effector is housed in and individual aluminum canister. Each canister contains a 0.2 μm Teflon filter to allow the interior volume to accommodate pressure changes without permitting microbial contaminants to enter the sterile interior volume. A handle repurposed from the space shuttle tile repair kit is used to remove the end effector from the sample canister, collect a sample by swabbing a surface and then replace the end effector in its canister. The canisters and end effectors were cleaned and assembled on Earth. Prior to installing the sterile swab the canisters and end effectors were sterilized in an autoclave at 134°C, 215 kPA, for 7 min.. The final assembly occurred in a sterilized class II biosafety cabinet. We will collect six samples from the 1) airlock vestibule, 2) airlock thermal cover, 3) a gap in the micrometeorite shielding near the airlock, 4) a handrail near the airlock, 5) the CDRA (Carbon Dioxide Removal Assembly) vent, and 6) the VES (Vacuum Exhaust System) vent. The remaining two swabs will be reserved as controls. One swab will be exposed during the EVA without touching any surfaces to act as a blank. The final swab will remain sealed until the entire sampling kit is returned to earth. Based on previously published results from the Russian segment, we hypothesize that there will be detectable microbes at some or all of these locations. Ground-based testing of this sampling caddy confirms that the swabs remain sterile as the canisters transition in and out of vacuum. We were able to retrieve, viable bacterial and fungal cells as well as DNA from samples collected from US space suits during vacuum chamber tests lasting as long as seven hours. Based on these results and feedback from the test subjects the sampling caddy was modified to improve ergonomics and meet US EVA safety requirements. Bayonet probes were added to the sides of the sample kit as alternate mounting points. Additional locking features were added to the end effector and the filter stack to prevent inadvertent release during use. The opening mechanism was changed from one where the end effector was rocked laterally to defeat a ball detent to a twist-to-open threaded closure for similar reasons. Demonstrating, this sampling caddy’s effectiveness during a US EVA will allow us to address knowledge gaps identified in COSPAR reports and begin to define planetary protection requirements for life support systems on crewed missions to mars. This kit could also be used to collect contamination control samples during Artemis missions to verify requirements and could be easily modified for robotic sample collection.

Planetary Protection↗