Results for “Trajectory Based Operations” — Page 16

NASA NTRS2018

Air Traffic Management Technology Demostration-1 (ATD-1) Avionics Phase 2 Flight Test and Results

NASA’s first Air Traffic Management Technology Demonstration (ATD-1) subproject successfully completed 19 days of flight test validation in January and February 2017 of an Interval Management (IM) avionics prototype and the procedures used to conduct IM arrival and approach operations. IM is one of the three elements integrated into NASA’s ATD-1 concept of operations with the subproject goal of improving aircraft efficiency and airport throughput during high-density arrival operations. The ATD-1 concept of operations combines advanced arrival scheduling, controller decision support tools, and interval management (IM) avionics to enable merging of multiple, time-based, efficient arrival streams. IM contributes to the operation by calculating speeds that enable an aircraft to precisely achieve a specific time or distance behind another aircraft. When precise spacing intervals can be calculated, achieved, and then maintained during high-density operations, aircraft efficiency should be improved by enabling the aircraft to remain closer to the optimum descent trajectory instead of using vectors and step-down altitudes, and airport throughput should be maintained or improved by each aircraft arriving at the runway threshold closer to the assigned spacing interval. This avionics development and flight test was conducted under a NASA contract by Boeing Research and Technology, with Boeing Commercial Aircraft, Honeywell, United Airlines, and Jeppesen as sub-contractors. The Honeywell built IM avionics were the first ever prototype built based on NASA requirements as well as developing and non-flight tested international IM standards, integrated into two test aircraft, and then flown in real-world conditions at the Grant County International Airport (KMWH). The IM prototype flown in the flight test used data from the Ownship and the assigned lead, or Target, aircraft to calculate the airspeed necessary for the Ownship to achieve the desired spacing. The flight test demonstrated that the IM avionics prototype generally met the IM requirement for spacing accuracy. However, the control laws implemented require further development to reduce the high IM speed command rate and the number of speed reversals observed during the test. Pilots assessed the IM procedure as acceptable, and issues requiring further attention were identified. In summary, the IM avionics prototype showed significant promise in contributing to the goals of improving aircraft efficiency and airport throughput. The flight test results also provided important data to the FAA and the working group developing the follow-on version of the international IM standards.

Baxley, Brian T.↗

NASA NTRS1997

Preliminary Design of Low-Thrust Interplanetary Missions

For interplanetary missions, highly efficient electric propulsion systems can be used to increase the mass delivered to the destination and/or reduce the trip time over typical chemical propulsion systems. This technology is being demonstrated on the Deep Space 1 mission - part of NASA's New Millennium Program validating technologies which can lower the cost and risk and enhance the performance of future missions. With the successful demonstration on Deep Space 1, future missions can consider electric propulsion as a viable propulsion option. Electric propulsion systems, while highly efficient, produce only a small amount of thrust. As a result, the engines operate during a significant fraction of the trajectory. This characteristic makes it much more difficult to find optimal trajectories. The methods for optimizing low-thrust trajectories are typically categorized as either indirect, or direct. Indirect methods are based on calculus of variations, resulting in a two-point boundary value problem that is solved by satisfying terminal constraints and targeting conditions. These methods are subject to extreme sensitivity to the initial guess of the variables - some of which are not physically intuitive. Adding a gravity assist to the trajectory compounds the sensitivity. Direct methods parameterize the problem and use nonlinear programming techniques to optimize an objective function by adjusting a set of variables. A variety of methods of this type have been examined with varying results. These methods are subject to the limitations of the nonlinear programming techniques. In this paper we present a direct method intended to be used primarily for preliminary design of low-thrust interplanetary trajectories, including those with multiple gravity assists. Preliminary design implies a willingness to accept limited accuracy to achieve an efficient algorithm that executes quickly.

Sims, Jon A.↗

NASA NTRS2001

On the Formal Verification of Conflict Detection Algorithms

Safety assessment of new air traffic management systems is a main issue for civil aviation authorities. Standard techniques such as testing and simulation have serious limitations in new systems that are significantly more autonomous than the older ones. In this paper, we present an innovative approach, based on formal verification, for establishing the correctness of conflict detection systems. Fundamental to our approach is the concept of trajectory, which is a continuous path in the x-y plane constrained by physical laws and operational requirements. From the Model of trajectories, we extract, and formally prove, high level properties that can serve as a framework to analyze conflict scenarios. We use the Airborne Information for Lateral Spacing (AILS) alerting algorithm as a case study of our approach.

Munoz, Cesar↗

NASA NTRS2013

The Single Habitat Module Concept for Exploration - Mission Planning and Mass Estimates

The Single Habitat Module (SHM) concept approach to the infrastructure and conduct of exploration missions combines many new promising technologies with a central concept of mission architectures that use a single habitat module for all phases of an exploration mission. Integrating mission elements near Earth and fully fueling them prior to departure of the vicinity of Earth provides the capability of using the single habitat both in transit to/from an exploration destination and while exploring the destination. The concept employs the capability to return the habitat and interplanetary propulsion system to Earth vicinity so that those elements can be reused on subsequent exploration missions. This paper provides an overview of the SHM concept and the advantages it provides. The paper also provides a summary of calculations of the mass of the Habitat Propulsion System (HPS) needed to get the habitat from low-Mars orbit (LMO) to the surface and back to LMO, and an overview of trajectory and mission mass assessments related to use of a high specific impulse space-based propulsion system. Those calculations led to the conclusion that the SHM concept results in low total mass required and streamlines mission operations to explore Mars (or other exploration destinations).

Chambliss, Joe↗

NASA NTRS2024

Use of Forecast Atmosphere for Earth Entry, Descent, and Landing Modeling

Flight mechanics simulations are used to characterize the performance of Earth entry, descent, and landing vehicles. Atmospheric prediction models are often a key component of these simulations. Global atmospheric models are used for both engineering design due to their ability to define atmospheric properties over a large swath of locations and times as well as used for the availability of atmospheric uncertainties in the models that can be used in statistical performance analysis. However for day-of-flight operations, the use of forecast atmospheres based on more current measurements are beneficial to the accuracy of the performance prediction, including landing locations. Reanalysis of these forecasts can also be used for post-flight analysis, including trajectory reconstruction. This paper describes how forecast atmospheres can be beneficial for Earth entry, descent, and landing analysis and ways these models can be implemented in simulations.

Soumyo Dutta↗

NASA NTRSDate not supplied

Vision-Based Distributed Sensing at Vertiports for Advanced Air Mobility and Urban Air Mobility Approach and Landing

Advanced Air Mobility (AAM) encompasses a broad vision for air transportation, including Urban Air Mobility (UAM) as a subset. AAM aims to create a more connected and efficient transportation network across various geographical settings. However, navigating AAM aircraft in GPS-denied or degraded environments during approach and landing is challenging. Traditional vision aids like glideslopes and localizers are limited in vertiport environments due to narrow beam constraints and reduced landing angle options. This paper addresses the need for accurate navigation solutions at vertiports by proposing a vision-based distributed sensing (VIDIS) system utilizing cameras with bundle adjustment to assist incoming AAM aircraft during approach and landing while monitoring surface movements to enhance safety and efficiency. Key focus areas for current and future vertiport developers include identifying suitable sensor types and infrastructure standards to support AAM operations and including vertiport markings as vision-based navigation aids. The proposed system offers a novel approach to overcoming navigation challenges in AAM operations, particularly in urban settings where traditional aids may be insufficient. Preliminary simulation results with distributed cameras demonstrate promising outcomes for implementing bundle adjustment techniques to enhance vision-based navigation solutions at vertiports. Generating waypoint-based trajectories via waypoint integration using explicit guidance synthesis (WINGS) creates smooth AAM trajectories for landing at vertiports by using the current waypoint's terminal conditions as the initial conditions for the next waypoint. Combining bundle adjustment's ground-based solution of vertiport features with WINGS, Coplanar Pose from Orthography and Scaling with Iterations (COPOSIT), and an extended Kalman filter (EKF) estimates the state of an incoming aircraft during approach and landing at vertiports. Future work includes testing VIDIS in a high-fidelity simulation and with real-world data.

Distributed sensing↗

NASA NTRSDate not supplied

Vision-Based Distributed Sensing at Vertiports for Advanced Air Mobility and Urban Air Mobility Approach and Landing

Advanced Air Mobility (AAM) encompasses a broad vision for air transportation, including Urban Air Mobility (UAM) as a subset. AAM aims to create a more connected and efficient transportation network across various geographical settings. However, navigating AAM aircraft in GPS-denied or degraded environments during approach and landing is challenging. Traditional vision aids like glideslopes and localizers are limited in vertiport environments due to narrow beam constraints and reduced landing angle options. This paper addresses the need for accurate navigation solutions at vertiports by proposing a vision-based distributed sensing (VIDIS) system utilizing cameras with bundle adjustment to assist incoming AAM aircraft during approach and landing while monitoring surface movements to enhance safety and efficiency. Key focus areas for current and future vertiport developers include identifying suitable sensor types and infrastructure standards to support AAM operations and including vertiport markings as vision-based navigation aids. The proposed system offers a novel approach to overcoming navigation challenges in AAM operations, particularly in urban settings where traditional aids may be insufficient. Preliminary simulation results with distributed cameras demonstrate promising outcomes for implementing bundle adjustment techniques to enhance vision-based navigation solutions at vertiports. Generating waypoint-based trajectories via waypoint integration using explicit guidance synthesis (WINGS) creates smooth AAM trajectories for landing at vertiports by using the current waypoint's terminal conditions as the initial conditions for the next waypoint. Combining bundle adjustment's ground-based solution of vertiport features with WINGS, Coplanar Pose from Orthography and Scaling with Iterations (COPOSIT), and an extended Kalman filter (EKF) estimates the state of an incoming aircraft during approach and landing at vertiports. Future work includes testing VIDIS in a high-fidelity simulation and with real-world data.

Distributed sensing↗

NASA NTRS1996

Decision-Aiding and Optimization for Vertical Navigation of Long-Haul Aircraft

Most decisions made in the cockpit are related to safety, and have therefore been proceduralized in order to reduce risk. There are very few which are made on the basis of a value metric such as economic cost. One which can be shown to be value based, however, is the selection of a flight profile. Fuel consumption and flight time both have a substantial effect on aircraft operating cost, but they cannot be minimized simultaneously. In addition, winds, turbulence, and performance vary widely with altitude and time. These factors make it important and difficult for pilots to (a) evaluate the outcomes associated with a particular trajectory before it is flown and (b) decide among possible trajectories. The two elements of this problem considered here are: (1) determining what constitutes optimality, and (2) finding optimal trajectories. Pilots and dispatchers from major u.s. airlines were surveyed to determine which attributes of the outcome of a flight they considered the most important. Avoiding turbulence-for passenger comfort-topped the list of items which were not safety related. Pilots' decision making about the selection of flight profile on the basis of flight time, fuel burn, and exposure to turbulence was then observed. Of the several behavioral and prescriptive decision models invoked to explain the pilots' choices, utility maximization is shown to best reproduce the pilots' decisions. After considering more traditional methods for optimizing trajectories, a novel method is developed using a genetic algorithm (GA) operating on a discrete representation of the trajectory search space. The representation is a sequence of command altitudes, and was chosen to be compatible with the constraints imposed by Air Traffic Control, and with the training given to pilots. Since trajectory evaluation for the GA is performed holistically, a wide class of objective functions can be optimized easily. Also, using the GA it is possible to compare the costs associated with different airspace design and air traffic management policies. A decision aid is proposed which would combine the pilot's notion of optimality with the GA-based optimization, provide the pilot with a number of alternative pareto-optimal trajectories, and allow him to consider unmodelled attributes and constraints in choosing among them. A solution to the problem of displaying alternatives in a multi-attribute decision space is also presented.

Patrick, Nicholas J. M.↗

NASA NTRS1996

Decision-Aiding and Optimization for Vertical Navigation of Long-Haul Aircraft

Most decisions made in the cockpit are related to safety, and have therefore been proceduralized in order to reduce risk. There are very few which are made on the basis of a value metric such as economic cost. One which can be shown to be value based, however, is the selection of a flight profile. Fuel consumption and flight time both have a substantial effect on aircraft operating cost, but they cannot be minimized simultaneously. In addition, winds, turbulence, and performance x,ary widely with altitude and time. These factors make it important and difficult for pilots to (a) evaluate the outcomes associated with a particular trajectory before it is flown and (b) decide among possible trajectories. The two elements of this problem considered here are (1) determining, what constitutes optimality, and (2) finding optimal trajectories. Pilots and dispatchers from major U.S. airlines were surveyed to determine which attributes of the outcome of a flight they considered the most important. Avoiding turbulence-for passenger comfort topped the list of items which were not safety related. Pilots' decision making about the selection of flight profile on the basis of flight time, fuel burn, and exposure to turbulence was then observed. Of the several behavioral and prescriptive decision models invoked to explain the pilots' choices, utility maximization is shown to best reproduce the pilots' decisions. After considering more traditional methods for optimizing trajectories, a novel method is developed using a genetic algorithm (GA) operating on a discrete representation of the trajectory search space. The representation is a sequence of command altitudes, and was chosen to be compatible with the constraints imposed by Air Traffic Control, and with the training given to pilots. Since trajectory evaluation for the GA is performed holistically, a wide class of objective functions can be optimized easily. Also, using the GA it is possible to compare the costs associated with different airspace design and air traffic management policies. A decision aid is proposed which would combine the pilot's notion of optimility with the GA-based optimization, provide the pilot with a number of alternative pareto-optimal trajectories, and allow him to consider un-modelled attributes and constraints in choosing among them. A solution to the problem of displaying alternatives in a multi-attribute decision space is also presented.

Patrick, Nicholas J. M.↗

NASA NTRS1990

A comparison of operationally determined atmospheric densities from satellite orbit solutions and the exospheric temperature from the Jacchia-Roberts model

Operational orbit determination by the Flight Dynamics Division at the Goddard Space Flight Center has yielded a data base of orbit solutions covering the onset of solar cycle 22. Solutions for nine satellites include an estimated drag adjustment parameter (rho sub 1) determined by the Goddard Trajectory Determination System (GTDS). The rho sub 1 is used to evaluate correlations between density variations and changes in the following: 10.7-centimeter wavelength solar flux (F sub 10.7), the geomagnetic index A sub p, and two exospheric temperatures (T sub c and T sub infinity) adapted from the Jacchia-Roberts atmospheric density model in GTDS. T sub c depends on the daily and 81-day centered mean F sub 10.7; T sub infinity depends on T sub c and the geomagnetic index K sub p values. The highest correlations are between density and T sub infinity. Correlations with T sub c and F sub 10.7 are lower by 9 and 10 percent, respectively. For most cases, correlations with A sub p are considerably lower; however, significant correlations with A sub p were found for some high-inclination, moderate-altitude orbits. Results from this analysis enhance the understanding of the drag model and the accommodation of atmospheric density variations in the operational orbit determination support. The degree of correlation demonstrates the sensitivity of the orbit determination process to drag variations and to the input parameters that characterize aspects of the atmospheric density model. To this extent, the degree of correlation provides a measure of performance for methods of selecting or modeling the thermospheric densities using the solar F sub 10.7 and geomagnetic data as input to the process.

Ward, D. T.↗

NASA NTRS1977

Radio frequency interference from near-earth satellites

A pessimistic statistical model was developed for predicting the extent of radio frequency interference (RF1). Based on the assumptions underlying the model, DSN S-band operations can expect one RF1 interruption every 4.1 days, with the average incident lasting 24 s. This implies that 52 or more such satellites, with uncorrelated orbital trajectories, will cause in excess of 5 min of RF1 per day at a DSN station.

Levitt, B. K.↗

NASA NTRS2013

The Single Habitat Module Concept for Exploration - Mission Planning and Mass Estimates

The Single Habitat Module (SHM) concept approach to the infrastructure and conduct of exploration missions combines many of new promising technologies with a central concept of mission architectures that use a single habitat module for all phases of an exploration mission. Integrating mission elements near Earth and fully fueling them prior to departure of the vicinity of Earth provides the capability of using the single habitat both in transit to/from an exploration destination and while exploring the destination. The concept employs the capability to return the habitat and interplanetary propulsion system to Earth vicinity so that those elements can be reused on subsequent exploration missions. This paper provides an overview of the SHM concept and the advantages it provides. A summary of calculations of the mass of the habitat propulsion system (HPS) needed to get the habitat from Low Mars Orbit (LMO) to the surface and back to LMO and an overview of trajectory and mission mass assessments related to use of a high specific impulse space based propulsion system is provided. Those calculations lead to the conclusion that the SHM concept can significantly reduce the mass required and streamline mission operations to explore Mars (and thus all exploration destinations).

Chambliss, Joe↗

NASA NTRSDate not supplied

Initial Design Guidelines for Onboard Automation of Flight Path Management

Achieving the National Academy of Science’s vision of advanced aerial mobility will depend on significant developments in automation to achieve safe and efficient operations. Flight path management (FPM), a major category of automation functionality needed to achieve this vision, will provide dynamic management of an aircraft’s flight path, ensuring that it remains feasible to fly to mission completion, deconflicted from hazards, coordinated with other traffic, flexible to accommodate future disturbances, and optimized to meet business objectives. While efforts are underway to advance FPM technology for the Urban Air Mobility application, initial design guidelines are presented for FPM automation capabilities to achieve each of these objectives based on 15+ years of prior FPM automation research and development. Methods to efficiently account for uncertainty in the prediction of trajectories are described, as are additional considerations for prioritizing safety in the design of FPM automation capabilities and interactions between aircraft. Recommendations are supported by extensive experience gained via previous work with the FPM reference automation system, Autonomous Operations Planner, developed by NASA. By employing capable FPM automation supported by cooperative operational flight rules and information sharing, future aircraft operators will benefit from an increased ability to plan and execute safe and efficient flights and to achieve mission success in a dynamic airspace.

Flight Path Management, FPM, AOP, UAM, deconflicti↗

NASA NTRSDate not supplied

Initial Design Guidelines for Onboard Automation of Flight Path Management

Achieving the National Academy of Science’s vision of advanced aerial mobility will depend on significant developments in automation to achieve safe and efficient operations. Flight path management (FPM), a major category of automation functionality needed to achieve this vision, will provide dynamic management of an aircraft’s flight path, ensuring that it remains feasible to fly to mission completion, deconflicted from hazards, coordinated with other traffic, flexible to accommodate future disturbances, and optimized to meet business objectives. While efforts are underway to advance FPM technology for the Urban Air Mobility application, initial design guidelines are presented for FPM automation capabilities to achieve each of these objectives based on 15+ years of prior FPM automation research and development. Methods to efficiently account for uncertainty in the prediction of trajectories are described, as are additional considerations for prioritizing safety in the design of FPM automation capabilities and interactions between aircraft. Recommendations are supported by extensive experience gained via previous work with the FPM reference automation system, Autonomous Operations Planner, developed by NASA. By employing capable FPM automation supported by cooperative operational flight rules and information sharing, future aircraft operators will benefit from an increased ability to plan and execute safe and efficient flights and to achieve mission success in a dynamic airspace.

Flight Path Management↗

NASA NTRS2019

Modeling Uncertainty in Time and Fuel Benefit Estimation for TASAR Operational Evaluation

The Traffic Aware Strategic Aircrew Requests concept aims to reduce weather-induced delays, improve route efficiency, and efficiently share route modification options by combining onboard avionics data, Automatic Dependent Surveillance-Broadcast data, and broadband internet data to generate optimal, traffic-compatible trajectory changes based on real-time traffic and weather data. Time and fuel benefits due to use of the Traffic Aware Planner (TAP) software can be estimated by taking the difference in predicted flight time and fuel usage before and after a TAP-inspired trajectory change is completed. Although TAP’s optimization algorithm predicts flight and fuel usage based on the current flight route and weather data, it does not account for possible air traffic controller-initiated trajectory changes, reroutes due to sudden weather changes, or other pilot/controller actions that may occur during flight. This paper introduces an approach for quantifying the uncertainty in estimated time and fuel benefits.

Burris, Joseph↗

NASA NTRS2018

Concept of Operations for Management by Trajectory

This document describes Management by Trajectory (MBT), a concept for future air traffic management (ATM) in which every flight operates in accordance with a four-dimensional trajectory (4DT) that is negotiated between the airspace user and the Federal Aviation Administration (FAA) to respect the airspace user's goals while complying with National Airspace System (NAS) constraints. In the present-day NAS, the ATM system attempts to predict the trajectory for each flight based on the approved flight plan and scheduled or controlled departure time. However, once the aircraft starts to move, controllers tactically manage the aircraft to implement traffic management restrictions, separate otherwise conflicting aircraft, and address arising NAS constraints. Tactical controller actions are not directly communicated to the automation systems or other stakeholders. Furthermore, the initial trajectory prediction does not anticipate these disruptions or how they will impact the flight. Consequently, and compounded by gaps in required data and models, trajectory predictions are less accurate than possible, which affects Traffic Flow Management (TFM) performance. A cornerstone of the MBT concept is that all air vehicles have, at all times, an assigned 4DT from their current state to their destination. These assigned trajectories consist of trajectory constraints and descriptions. Pilots and air traffic controllers, with the aid of automation, operate the aircraft to comply with the assigned trajectory, unless first negotiating a revision. Equipped aircraft have substantial responsibility for complying with the assigned trajectory without controller intervention. To maximize the operational flexibility available to the airspace user, the assigned trajectory only imposes trajectory constraints as required to achieve the ATM goals of NAS constraint compliance and aircraft separation. Trajectory descriptions are added to the assigned trajectory to ensure sufficient predictability. To further improve trajectory prediction accuracy, airspace users supplement the assigned trajectory by broadcasting intent information and updating it as necessary. Air vehicle intent is a more detailed description of the airspace user's plan for how the flight will fly the assigned trajectory. Air vehicle intent can change freely, without negotiation, as long as it remains in compliance with the assigned trajectory. Aircraft assigned trajectories, air vehicle intent, and predicted trajectories are shared, creating a common view among stakeholders. A NAS Constraint Service gathers and publishes information about all known NAS constraints, enabling airspace users to be informed participants in trajectory negotiation. Trajectory constraints in the assigned trajectory are mapped to NAS constraints to facilitate identifying which aircraft are affected when NAS constraints change. To support efficient trajectory negotiation, all aircraft provide current information about air vehicle capabilities. Assigned trajectories are constructed to satisfy all known NAS constraints, improving trajectory stability and predictability. Uncertainty and disruptions are handled by modifying the assigned trajectory as far in advance as possible. By proactively negotiating changes to the assigned trajectory, rather than relying on controller-selected tactical actions such as vectors to resolve traffic conflicts or implement miles-in-trail restrictions, MBT keeps aircraft on closed trajectories that are fully known to all stakeholders. Since reactive air traffic control actions cannot be predicted in advance, the downstream trajectory cannot be accurately predicted until they happen. Reliable trajectory predictions allow the system to identify needed modifications to trajectories further in advance, where they can be negotiated and communicated as amendments (i.e., additional or altered trajectory constraints) to the assigned trajectory. Decision Support Tools (DSTs) aid controllers in rapidly defining and communicating closed trajectories to the aircraft and support all stakeholders in trajectory negotiation. Anticipated MBT benefit mechanisms include more accurate trajectory predictions, improved ATM performance and robustness to off-nominal conditions, increased flexibility and operational efficiency, reduced impediments to emerging classes of airspace users accessing NAS resources, reduced environmental impacts, and enhanced safety.

ConOps↗

NASA NTRS1983

On the feasibility of real-time prediction of aircraft carrier motion at sea

Landing aircraft on board carriers is a most delicate phase of flight operations at sea. The ability to predict the aircraft carrier's motion over an interval of several seconds within reasonable error bounds may allow an improvement in touchdown dispersion and reduce the value of the ramp clearance due to a smoother aircraft trajectory. Also, improved information to the landing signal officer should decrease the number of waveoffs substantially. This paper indicates and shows quantitatively that, based upon the power density spectrum data for pitch and heave measured for various ships and sea conditions, the motion can be predicted well, for up to 15 s. Moreover, the zero crossover times for both pitch and heave motions can be predicted with impressive accuracy. The predictor was designed on the basis of Kalman's optimum filtering theory (the discrete time case), being compatible with real-time digital computer operation.

Sidar, M. M.↗

DOE OSTI2026

Higher-order interaction effects among operating conditions and feedstocks shape reactor microbiomes and fatty acid production profiles

Arrested anaerobic digestion (AAD) offers a promising route for producing fatty acids (FAs) from organic residues, yet optimal conditions for selectively generating medium-chain fatty acids (MCFAs) remain poorly defined. Here, we systematically evaluated the main and interaction effects of pH (5, 7, 9), feedstock (food waste, manure), temperature (35 and 45 °C), and inoculum source on microbiome composition and FA production. Anaerobic digester sludge and a novel bison rumen inoculum were compared. Significant higher-order interactions among operating parameters governed FA profiles and microbiome structure. Butyric acid production was driven by a three-way interaction among pH, feedstock, and temperature (p < 0.001), with maximum concentrations achieved in food waste reactors at pH 5.0 and 35 °C (1.2 ± 0.1 g L −1 with sludge and 1.1 ± 0.3 g L −1 with rumen). MCFA production exhibited significant four-way interactions (p < 0.1 to p < 0.001). At 45 °C and pH 5.0, inoculum source tuned MCFA selectivity: sludge favored pentanoic acid (0.4 ± 0.1 g L −1 ), whereas rumen favored hexanoic and heptanoic acids (up to 0.4 ± 0.2 g L −1 ). Manure reactors produced < 0.2 g L −1 MCFAs under all conditions. Genera, including Megasphaera, Prevotella, and Lactobacillus, were associated with production of specific MCFAs. PICRUSt2-based pathway predictions were consistent with MCFA production patterns and suggested a potential role for lactic acid–driven chain elongation pathways. This study provides insights into how interacting operating conditions shape AAD microbiomes, their FA profiles, and advances the trajectory of research aimed at engineering robust and controllable microbiomes for waste valorization.

09 BIOMASS FUELS↗