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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 289 records · Page 16
All sourcesNASA NTRSDOE OSTI
NASA NTRS1975

Analytical method for determining the stability of linear retarded systems with two delays

The stability is considered of the solution differential-difference equations of the retarded type with constant coefficients and two constant time delays. A method that makes use of analytical expressions to determine stability boundaries, and the stability of the system, is derived. The method was applied to a system represented by a second-order differential equation with constant coefficients and time delays in the velocity and displacement terms. The results obtained is in agreement with those obtained by other investigators.

Barker, L. K.↗
NASA NTRS2019

The Omics of Stem Cell Mediated Regeneration: A Pilot Single Cell RNA-Seq Study of Mechanotransduction

Mechanical forces are potent modulators of stem cell based tissue regenerative mechanisms, inducing cell fate decisions and tissue specific commitment. A unique platform for investigating mechanotransduction is spaceflight, where microgravity and altered fluid mechanics provide a loading-null experimental condition. Seminal investigations of regenerative capacity in a wholly regenerative species, the newt model, and in a variety of totipotent and adult stem cell populations have demonstrated the detrimental effects of unloading on maintenance of stem cell based regeneration. Of particular interest is the observation that unloading interferes with the transition of stem cell pools from proliferative state to differentiation commitment. In this work we sought to test the hypothesis that gravity mechanotransduction regulates stem cell tissue regenerative processes by modulating stem cell proliferation and differentiation fates at specific cell cycle stages. To do this, clonally-derived ESCs were plated on a collagen matrix and expanded for 36 hours before re-plating on a non-adherent culture dish in the absence of leukemia inhibitory factor (LIF) to form spheroid aggregate EBs. After formation, the EBs were transferred to a collagen matrix coated culture dishes and given 4 days to allow implantation and outgrowth. In parallel, totipotent ESCs were plated 24 hours before mechanical stimulation on collagen matrix culture dishes in the presence of LIF to maintain totipotency and serve as un-differentiation committed controls. The EBs and ESCs were then subjected to either a 60 minute pulse of gravity (static loading) or 60 minutes of cyclic stretch (dynamic loading) mechanotransduction. Six hours post-stimulation, we used a 10X Genomics Single Cell controller to generate bar-coded single cell Illumina libraries and sequenced expressomes for 5,000 static loaded cells, representative of a change in gravity mechanotransduction, 5,000 dynamic loaded cells, representative of tissue loading associate with physiologic function, and 5,000 unstimulated 1g control cells. The comparison of these 3 libraries by cluster assignment based on like gene expression patterns show substantial alteration in cluster geometry due to mechanical loading. Specifically the mechanically loaded EB outgrowth cells to retain potency markers (PAX6, SOX2, CD34) and suppress early commitment markers (Dhh, VCAN, Igf1). Whereas the EBs cultured under the non-stimulated conditions display clear departure from the ESC expressome with lineage commitment markers upregulated and several tissue specific markers being expressed (BMP "early musculoskeletal development, Mesp1" early cardiovascular cell lineage). These markers are not seen in the mechano-stimulated cultures or the totipotent ESC cultures. Comparison of like clusters between our experimental conditions revealed an array of regenerative and stem cell genes are significantly mechano-regulated. Of particular importance CDKN1a/p21, a gene shown by previous investigation of our research team to be significantly upregulated in unloading, was suppressed in the static and dynamic loaded EBS. In addition to CDKN1a/p21 many genes related to cell cycle and transitory differentiation markers had elevated expression in the mechano-stimulated EBs, but surprisingly these trends were not observed in the ESC cultures. This study is the first of its kind investigating for mechano-signaling and mechano-regulated pathways, and has alre

Single cell RNA-sequecing↗
NASA NTRS1976

Approximate solutions of radiative transfer in dusty nebulae. II - Hydrogen and helium

The ionization structure of hydrogen and helium in dusty nebulae is discussed. General equations for pure hydrogen nebulae have been modified to include helium. An approximate analytical solution is presented for Stroemgren radii of hydrogen and helium in the absence of dust. It is shown that these results, with simple modifications, are also applicable to dusty nebulae where the effective absorption cross section of dust grains varies slowly with frequency in the range from 1000 to 200 A. No analytical solutions are possible if this cross section varies rapidly with frequency. In this case, however, simple coupled differential equations are derived which can easily be solved numerically. Approximate analytical expressions are given for evaluating the variation of the fraction of ionizing radiation absorbed by dust and the ratio of the volume emission measures of He II to H II regions with the spectrum of the ionizing source, helium abundance, and absorption properties of dust. The effects of dust on the He III zone are discussed. The present results are restricted to spherically symmetric nebulae, but nonuniform gas and dust distributions and clumpiness can be taken into account by the general results.

Dana, R. A.↗
NASA NTRS1984

Discrete orthogonal function expansions for non-uniform grids using the fast Fourier transform

A technique for applying discrete Fourier series to infinite domains is presented. The technique uses mappings designed to minimize truncation error and can be applied to solve mixed initial boundary value problems among others. The method is alias-free and yields consistent differentiation and integration operators. The mapping-induced truncation error is explicitly expressible and small in nearly all cases of interest. The method is illustrated for three problems involving convection, diffusion, and vortex interaction.

Cain, A. B.↗
NASA NTRS1991

Likelihood estimation for distributed parameter models for NASA Mini-MAST truss

A maximum likelihood estimation for distributed parameter models of large flexible structures was formulated. Distributed parameter models involve far fewer unknown parameters than independent modal characteristics or finite element models. The closed form solutions for the partial differential equations with corresponding boundary conditions were derived. The closed-form expressions of sensitivity functions led to highly efficient algorithms for analyzing ground or on-orbit test results. For an illustration of this approach, experimental data of the NASA Mini-MAST truss was used. The estimations of modal properties involve lateral bending modes and torsional modes. The results show that distributed parameter models are promising in the parameter estimation of large flexible structures.

Shen, Ji-Yao↗
NASA NTRS1992

Likelihood estimation for distributed parameter models of large beam-like structures

A maximum likelihood estimation for distributed parameter models of large flexible structures was formulated. Distributed parameter models involve far fewer unknown parameters than independent modal characteristics or finite element models. The closed form solutions for the partial differential equations with corresponding boundary conditions were derived. The closed-form expressions of sensitivity functions led to highly efficient algorithms for analyzing ground or on-orbit test results. For an illustration of this approach, experimental data of the NASA Mini-Mast truss was used. The estimations of modal properties involve lateral bending modes and torsional modes. The results show that distributed parameter models are promising in the parameter estimation of large flexible structures.

Shen, J.-Y.↗
NASA NTRS1997

The solid state environment orchestrates embryonic development and tissue remodeling

Cell interactions with extracellular matrix and with other cells play critical roles in morphogenesis during development and in tissue homeostasis and remodeling throughout life. Extracellular matrix is information-rich, not only because it is comprised of multifunctional structural ligands for cell surface adhesion receptors, but also because it contains peptide signaling factors, and proteinases and their inhibitors. The functions of these groups of molecules are extensively interrelated. In this review, three primary cell culture models are described that focus on adhesion receptors and their roles in complex aspects of morphogenesis and remodeling: the regulation of proteinase expression by fibronectin and integrins in synovial fibroblasts; the regulation of osteoblast differentiation and survival by fibronectin, and the regulation of trophoblast differentiation and invasion by integrins, cadherins and immunoglobulin family adhesion receptors.

NASA Discipline Musculoskeletal↗
NASA NTRS2006

Three-dimensional Myoblast Aggregates--Effects of Modeled Microgravity

The overall objective of these studies is to elucidate the molecular and cellular alterations that contribute to muscle atrophy in astronauts caused by exposure to microgravity conditions in space. To accomplish this, a three-dimensional model test system was developed using mouse myoblast cells (C2C12). Myoblast cells were grown as three-dimensional aggregates (without scaffolding or other solid support structures) in both modeled microgravity (Rotary Cell Culture System, Synthecon, Inc.) and at unit gravity in coated Petri dishes. Evaluation of H&E stained thin sections of the aggregates revealed the absence of any necrosis. Confocal microscopy evaluations of cells stained with the Live/Dead assay (Molecular Probes) confirmed that viable cells were present throughout the aggregates with an average of only three dead cells observed per aggregate. Preliminary results from gene array analysis (Affymetrix chip U74Av2) showed that approximately 14% of the genes were down regulated (decreased more than 3 fold) and 4% were upregulated in cells exposed to modeled microgravity for 12 hours compared to unit gravity controls. Additional studies using fluorescent phallacidin revealed a decrease in F-actin in the cells exposed to modeled microgravity compared to unit gravity. Myoblast cells grown as aggregates in modeled microgravity exhibited spontaneous differentiation into syncitia while no differentiation was seen in the unit gravity controls. These studies show that 1)the model test system developed is suitable for assessing cellular and molecular alterations in myoblasts; 2) gene expression alterations occur rapidly (within 12 hours) following exposure to modeled microgravity; and 3) modeled microgravity conditions stimulated myoblast cell differentiation. Achieving a greater understanding of the molecular alterations leading to muscle atrophy will eventually enable the development of cell-based countermeasures, which may be valuable for treatment of muscle diseases on Earth and future space explorations.

Byerly, Diane↗
NASA NTRS2015

On Small Disturbance Ascent Vent Behavior

As a spacecraft undergoes ascent in a launch vehicle, its ambient pressure environment transitions from one atmosphere to high vacuum in a matter of a few minutes. Venting of internal cavities is necessary to prevent the buildup of pressure differentials across cavity walls. These pressure differentials are often restricted to low levels to prevent violation of container integrity. Such vents usually consist of fixed orifices, ducts, or combinations of both. Duct conductance behavior is fundamentally different from that for orifices in pressure driven flows governing the launch vehicle ascent depressurization environment. Duct conductance is governed by the average pressure across its length, while orifice conductance is dictated by a pressure ratio. Hence, one cannot define a valid equivalent orifice for a given duct across a range of pressure levels. This presentation discusses development of expressions for these two types of vent elements in the limit of small pressure differentials, explores conditions for their validity, and compares their features regarding ascent depressurization performance.

Venting↗
NASA NTRS1994

The Teton fault, Wyoming: Topographic signature, neotectonics, and mechanisms of deformation

We integrated geophysical and geological methods to evalute the structural evolution of the active Teton normal fault, Wyoming, and its role in the development of the dramatic topography of Teton Range and Jackson Hole. Comparison of variations in surface offsets with the topographic expression of the Teton range crest and drainage divide, and the overall structure of the range, suggests that the effects ofpostglacial faulting cannot be discriminated from the influence of pre-extensional structures and differential; erosion on the footwall topography. In contrast, the effects of multiple scarp-forming normal faulting earthquakes are expressed by the anomalous drainage pattern and westward tilt of the hanging wall, Jackson Hole, toward the Teton fault. Kinematic boundary element fault models suggest that the westward tilt of the valley floor is the product of 110-125 m of displacement on a 45 deg-75 deg E dipping Teton fault in the past 25,000-75,000 years. Comparisons with historic normal faulting earthquake displacements imply that this range of displacement corresponds to 10-50, M greater than 7 scarp-forming earthquakes. A total throw of 2.5 to 3.5 km across the Teton fault is suggested by inverse ray-tracing and forward gravity models. These models also suggest that Laramide age structures have been offset across the Teton fault and obscure its geophysical signature but also continue to influence the structural and topographic expression of the footwall and hanging wall blocks. Paleomagnetic analyses of the approximately 2.0 Ma Huckelberry Ridge Tuff suggest that the overall westward tilt of the Teton Range is a result ofabout 10 deg of west side down tilt across the Teton fault since tuff emplacement. This suggests that much if not all of the throw across the Teton fault has accumulated in the past 2 m.y. Complex demagnetization and rock magnetic behavior and local emplacement of the Huckleberry Ridge Tuff on preexisting topogrpahy preclude determination of the amount or variations in throw along strike of the Teton fault from the paleomagnetic data.

Byrd, John O. D.↗
NASA NTRS2017

The Role of CDKN1a/p21 in Cellular Senescence of Bone Marrow Stem Cells Under Spaceflight Stressors

Spaceflight environments and their associated conditions, such as microgravity and space radiation, cause many biological functions formerly considered to be standard to behave in nonstandard ways. Exposure to microgravity has shown to induce deleterious effects in stem cell-based tissue regeneration, leading to immune system and healing response impairments as well as muscle and bone density loss. Such risks must be mitigated in order for long-term human space exploration to proceed. Thus, our work seeks to explore mechanisms of stem cell-based tissue regeneration that experience changes in spaceflight environments. Cellular senescence is a process of inducing cell cycle arrest that can be initiated by various stimuli. This function is influenced by two major pathways, controlled by p53 and pRB tumor suppressor proteins. p53 activity targets the cyclin-dependent kinase inhibitor gene p21Cdkn1a in osteogenic cell cycle arrest. Under conditions of mechanical unloading, stem cell-based tissue regeneration has shown to be decreased in both proliferation and differentiation, as many cells are arrested in progenitor states. p21 has shown upregulation in expression under conditions of microgravity, suggesting its role in regenerative bone formation arrest in space. p21 levels are found to be elevated independent of p53, suggesting a decrease in proliferation and regeneration without apoptosis, but rather through cell cycle arrest alone. Thus, we hypothesize that p21 is a mediator of cellular senescence in bone marrow stem cells. Culturing of bone marrow stem cells from wild type and p21 knockout mice under osteoblastogenic conditions will be completed to explore the role of p21Cdkn1a in stem cell proliferation and maturation. We believe that decreases in somatic stem cell differentiation may occur after spaceflight due to signal pathway alterations that result in downstream inhibition of genes involved in differentiation, preventing tissue from repairing and regenerating normally.

Stem Cells↗
NASA NTRS1998

Chimeric calcium/calmodulin-dependent protein kinase in tobacco: differential regulation by calmodulin isoforms

cDNA clones of chimeric Ca2+/calmodulin-dependent protein kinase (CCaMK) from tobacco (TCCaMK-1 and TCCaMK-2) were isolated and characterized. The polypeptides encoded by TCCaMK-1 and TCCaMK-2 have 15 different amino acid substitutions, yet they both contain a total of 517 amino acids. Northern analysis revealed that CCaMK is expressed in a stage-specific manner during anther development. Messenger RNA was detected when tobacco bud sizes were between 0.5 cm and 1.0 cm. The appearance of mRNA coincided with meiosis and became undetectable at later stages of anther development. The reverse polymerase chain reaction (RT-PCR) amplification assay using isoform-specific primers showed that both of the CCaMK mRNAs were expressed in anther with similar expression patterns. The CCaMK protein expressed in Escherichia coli showed Ca2+-dependent autophosphorylation and Ca2+/calmodulin-dependent substrate phosphorylation. Calmodulin isoforms (PCM1 and PCM6) had differential effects on the regulation of autophosphorylation and substrate phosphorylation of tobacco CCaMK, but not lily CCaMK. The evolutionary tree of plant serine/threonine protein kinases revealed that calmodulin-dependent kinases form one subgroup that is distinctly different from Ca2+-dependent protein kinases (CDPKs) and other serine/threonine kinases in plants.

NASA Discipline Plant Biology↗
NASA NTRS1985

The combined effects of stress and moisture on the structural integrity of composites

A variational approach is used to model the moisture content and distribution profiles which cause swelling in polymers and composites which are used as aircraft structural components. The moisture intrusion stems from humidity, which makes the problem one of diffusion of mass from the environment into a semiinfinite plate with a given diffusion coefficient and thickness. Lagrangian, ordinary differential equations are defined for the mass diffusion, including driving force, mass potential and volume dissipation expressions. Account is taken of the effects of stress on diffusivity, including the dilatational stress caused by inhomogeneous swelling of the material. It is noted that material near the top and bottom of a part undergoes the most swelling, and the evolution of moisture diffusion in the materials is traced. Finally, experimental data are used to define differential equations for predicting the final moisture distribution within the material.

Hiel, C.↗
NASA NTRS1997

Neuromuscular Development and Regulation of Myosin Expression

The proposed experiments were designed to determine whether the absence of gravity during embryogenesis influences the postnatal development of the neuromuscular system. Further, we examined the effects of reduced gravity on hindlimb muscles of the pregnant rats. Microgravity may have short and long-term effects on the development of muscle fiber type differentiation and force producing capabilities. Microgravity will reduce muscle fiber size and cause a shift in myosin heavy chain expression from slow to fast in hindlimb muscles of the adult pregnant rats.

Bodine, Sue↗
NASA NTRS1972

Longitudinal dynamic stability of a shuttle vehicle.

Analytical study of the longitudinal dynamic stability of a nonrolling, lifting vehicle gliding at hypersonic speeds. The analysis applies to shuttle vehicles designed for operating up to the rim of a planetary atmosphere. A general nondimensional time transformation is introduced to derive a unified second-order linear differential equation for the angle of attack, valid for all types of reentry of a general type of vehicle. The stability of motion is discussed for two fundamental regimes of flight that are based on widely different assumptions. For near ballistic entry along a straight line trajectory, the equation reduces to a confluent hypergeometric equation, the solution of which can be expressed in terms of Whittaker's function. Using a theorem in the theory of stability of differential equations, criteria for damped oscillations are derived. It is shown that the aerodynamic criteria for stability are the same as for the case of ballistic entry. In addition, for each vehicle configuration, and specified planetary atmosphere, there exists an altitude range where the angle of attack frequency is nearly equal to the orbital frequency causing instability in pitch. This resonance instability is due to the ellipticity of the orbit. Criteria for eccentricity instability are derived.

Vinh, N. X.↗
NASA NTRS2002

Saccharomyces cerevisiae gene expression changes during rotating wall vessel suspension culture

This study utilizes Saccharomyces cerevisiae to study genetic responses to suspension culture. The suspension culture system used in this study is the high-aspect-ratio vessel, one type of the rotating wall vessel, that provides a high rate of gas exchange necessary for rapidly dividing cells. Cells were grown in the high-aspect-ratio vessel, and DNA microarray and metabolic analyses were used to determine the resulting changes in yeast gene expression. A significant number of genes were found to be up- or downregulated by at least twofold as a result of rotational growth. By using Gibbs promoter alignment, clusters of genes were examined for promoter elements mediating these genetic changes. Candidate binding motifs similar to the Rap1p binding site and the stress-responsive element were identified in the promoter regions of differentially regulated genes. This study shows that, as in higher order organisms, S. cerevisiae changes gene expression in response to rotational culture and also provides clues for investigations into the signaling pathways involved in gravitational response.

Non-NASA Center↗