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NASA NTRS2011

Interplanetary Overlay Network Bundle Protocol Implementation

The Interplanetary Overlay Network (ION) system's BP package, an implementation of the Delay-Tolerant Networking (DTN) Bundle Protocol (BP) and supporting services, has been specifically designed to be suitable for use on deep-space robotic vehicles. Although the ION BP implementation is unique in its use of zero-copy objects for high performance, and in its use of resource-sensitive rate control, it is fully interoperable with other implementations of the BP specification (Internet RFC 5050). The ION BP implementation is built using the same software infrastructure that underlies the implementation of the CCSDS (Consultative Committee for Space Data Systems) File Delivery Protocol (CFDP) built into the flight software of Deep Impact. It is designed to minimize resource consumption, while maximizing operational robustness. For example, no dynamic allocation of system memory is required. Like all the other ION packages, ION's BP implementation is designed to port readily between Linux and Solaris (for easy development and for ground system operations) and VxWorks (for flight systems operations). The exact same source code is exercised in both environments. Initially included in the ION BP implementations are the following: libraries of functions used in constructing bundle forwarders and convergence-layer (CL) input and output adapters; a simple prototype bundle forwarder and associated CL adapters designed to run over an IPbased local area network; administrative tools for managing a simple DTN infrastructure built from these components; a background daemon process that silently destroys bundles whose time-to-live intervals have expired; a library of functions exposed to applications, enabling them to issue and receive data encapsulated in DTN bundles; and some simple applications that can be used for system checkout and benchmarking.

Burleigh, Scott C.↗

NASA NTRS2014

A Novel Protocol for Decoating and Permeabilizing Bacterial Spores for Epifluorescent Microscopy

Based on previously reported procedures for permeabilizing vegetative bacterial cells, and numerous trial-and-error attempts with bacterial endospores, a protocol was developed for effectively permeabilizing bacterial spores, which facilitated the applicability of fluorescent in situ hybridization (FISH) microscopy. Bacterial endospores were first purified from overgrown, sporulated suspensions of B. pumilus SAFR-032. Purified spores at a concentration of approx equals 10 million spores/mL then underwent proteinase-K treatment, in a solution of 468.5 μL of 100 mM Tris-HCl, 30 μL of 10% SDS, and 1.5 microL of 20 mg/mL proteinase-K for ten minutes at 35 ºC. Spores were then harvested by centrifugation (15,000 g for 15 minutes) and washed twice with sterile phosphate-buffered saline (PBS) solution. This washing process consisted of resuspending the spore pellets in 0.5 mL of PBS, vortexing momentarily, and harvesting again by centrifugation. Treated and washed spore pellets were then resuspended in 0.5 mL of decoating solution, which consisted of 4.8 g urea, 3 mL Milli-Q water, 1 mL 0.5M Tris, 1 mL 1M dithiothreitol (DTT), and 2 mL 10% sodium-dodecylsulfate (SDS), and were incubated at 65 ºC for 15 minutes while being shaken at 165 rpm. Decoated spores were then, once again, washed twice with sterile PBS, and subjected to lysozyme/mutanolysin treatment (7 mg/mL lysozyme and 7U mutanolysin) for 15 minutes at 35 C. Spores were again washed twice with sterile PBS, and spore pellets were resuspended in 1-mL of 2% SDS. This treatment, facilitating inner membrane permeabilization, lasted for ten minutes at room temperature. Permeabilized spores were washed two final times with PBS, and were resuspended in 200 mkcroL of sterile PBS. At this point, the spores were permeable and ready for downstream processing, such as oligonucleotideprobe infiltration, hybridization, and microscopic evaluation. FISH-microscopic imagery confirmed the effective and efficient (≈50% successful permeabilization and recovery) permeabilization of numerous spore preparations. The novelty of the technology developed here is in its applicability to bacterial endospores. While protocols abound for the effective permeabilization of bacterial, archaeal, and eukaryotic vegetative cells, there are no such reliable methods for decoating and permeabilizing bacterial endospores in a manner that is amenable to downstream FISH microscopic analyses. This innovation enables the direct visualization and enumeration of spores via FISH-based microscopic techniques, circumventing the complications that accompany previously required germination regimes. The synergistic enzymatic weakening of the many spore layers facilitates a structural compromise that is just enough to render the spores permeable without degrading the spore to a level, which precludes it from recognition.

LaDuc, Myron T.↗

NASA NTRS2015

NASA's International Space Station: A Testbed for Planetary Protection Protocol Development

Wherever humans go, they inevitably carry along the critters that live in and on them. Conventional wisdom has long held that it is unlikely those critters could survive the space environment, but in 2007 some microscopic aquatic animals called Tardigrades survived exposure to space and in 2008 Cyanobacteria lived for 548 days outside the ISS. Unlike the Mars rovers that were cleaned once and sent on their way, crew members will provide a constantly regenerating contaminant source. Are we prepared to certify that we can meet forward contamination protocols as we search for life at new destinations? What about the organisms we might reasonably expect a crewed spacecraft to leak or vent? Do we even know what they are? How long might our tiny hitch-hikers survive in close proximity to a warm spacecraft that periodically leaks/vents water or oxygen and how might they mutate with long-duration exposure? How will these contaminants migrate from their source in conditions encountered in space or on other planetary surfaces? This project aims to answer some of these questions by bringing together key stakeholder communities to develop a human forward contamination test, analysis, and integration plan. A system engineering approach to identify the experiments, analysis, and modeling needed to develop the contamination control protocols required will be used as a roadmap to integrate the many different parts of this problem - from launch to landing, living, and working on another planetary surface.

Bell, M. S.↗

NASA NTRS2015

Nasa's International Space Station: A Testbed for Planetary Protection Protocol Development

Bell, M. S.↗

NASA NTRS2015

Development of Test Protocols for International Space Station Particulate Filters

Air quality control on the International Space Station (ISS) is a vital requirement for maintaining a clean environment for the crew and the hardware. This becomes a serious challenge in pressurized space compartments since no outside air ventilation is possible, and a larger particulate load is imposed on the filtration system due to lack of gravitational settling. The ISS Environmental Control and Life Support System (ECLSS) uses a filtration system that has been in use for over 14 years and has proven to meet this challenge. The heart of this system is a traditional High-Efficiency Particulate Air (HEPA) filter configured to interface with the rest of the life support elements and provide effective cabin filtration. The filter element for this system has a non-standard cross-section with a length-to-width ratio (LW) of 6.6. A filter test setup was designed and built to meet industry testing standards. A CFD analysis was performed to initially determine the optimal duct geometry and flow configuration. Both a screen and flow straighter were added to the test duct design to improve flow uniformity and face velocity profiles were subsequently measured to confirm. Flow quality and aerosol mixing assessments show that the duct flow is satisfactory for the intended leak testing. Preliminary leak testing was performed on two different ISS filters, one with known perforations and one with limited use, and results confirmed that the testing methods and photometer instrument are sensitive enough to detect and locate compromised sections of an ISS BFE.Given the engineering constraints in designing spacecraft life support systems, it is anticipated that non-industry standard filters will be required in future designs. This work is focused on developing test protocols for testing the ISS BFE filters, but the methodology is general enough to be extended to other present and future spacecraft filters. These techniques for characterizing the test duct and perform leak testing can be applied to conducting acceptance testing and inventory testing for future manned exploration programs with air revitalization filtration needs, possibly even for in-situ filter element integrity testing for extensively long-duration missions. We plan to address the unique needs for test protocols for crewed spacecraft particulate filters by preparing the initial version of a standard, to be documented as a NASA Technical Memorandum (TM).

Filtration↗

NASA NTRS2015

Finalizing the CCSDS Space-Data Link Layer Security Protocol: Setup and Execution of the Interoperability Testing

The protection of data transmitted over the space-link is an issue of growing importance also for civilian space missions. Through the Consultative Committee for Space Data Systems (CCSDS), space agencies have reacted to this need by specifying the Space Data-Link Layer Security (SDLS) protocol which provides confidentiality and integrity services for the CCSDS Telemetry (TM), Telecommand (TC) and Advanced Orbiting Services (AOS) space data-link protocols. This paper describes the approach of the CCSDS SDLS working group to specify and execute the necessary interoperability tests. It first details the individual SDLS implementations that have been produced by ESA, NASA, and CNES and then the overall architecture that allows the interoperability tests between them. The paper reports on the results of the interoperability tests and identifies relevant aspects for the evolution of the test environment.

Fischer, Daniel↗

NASA NTRS2017

EVA Human Health and Performance Benchmarking Study Overview and Development of a Microgravity Protocol

The primary objective of this study is to develop a protocol to reliably characterize human health and performance metrics for individuals working inside various EVA suits under realistic spaceflight conditions. Expected results and methodologies developed during this study will provide the baseline benchmarking data and protocols with which future EVA suits and suit configurations (e.g., varied pressure, mass, center of gravity [CG]) and different test subject populations (e.g., deconditioned crewmembers) may be reliably assessed and compared. Results may also be used, in conjunction with subsequent testing, to inform fitness-for-duty standards, as well as design requirements and operations concepts for future EVA suits and other exploration systems.

Norcross, Jason↗

NASA NTRS2018

Use of Nack Oriented Reliable Multicast (NORM) Protocol for Transport of Spacecraft Telemetry in Ground Networks

Frequently it is necessary to distribute spacecraft telemetry to multiple destinations on Internet Protocol (IP) networks. There are various methods of delivering this data, but when the data is high rate and there are many destinations IP multicast provides the best solution from a network perspective. However, IP multicast only supports the User Datagram Protocol (UDP) which is connectionless and unreliable. This nature of UDP and by extension the low priority treatment of UDP packets by some network hardware creates a difficult environment for distribution of high rate telemetry to a number of recipients over IP networks. NACK (Negative ACKnowledgment) Oriented Reliable Multicast (NORM) is defined by IETF (Internet Engineering Task Force) RFC (Request for Comments) 5740 in 2009 by B. Adamson et al. NORM provides a mechanism of retransmission and or forward error correction for UDP packets, potentially overcoming the limits of UDP for the transmission of spacecraft telemetry. Marshall Space Flight Center’s (MSFC) Huntsville Operations Support Center (HOSC) manages the delivery of the International Space Station (ISS) payload science and health data. The ISS IP Ground Routed (IIGoR) network provides the delivery of the data from the ground stations to the control centers. HOSC personnel have been experimenting with the use of NORM on the link between the ground stations and the front end processor equipment in Huntsville, AL.

Baldwin, Patrick A.↗

NASA NTRS2019

Consensus on Aquatic Primary Productivity Field Protocols for Satellite Validation and Model Synthesis

The NASA PACE project, in conjunction with the IOCCG, EUMETSAT, and JAXA, have initiated an Aquatic Primary Productivity working group, with the aim to develop community consensus on multiple methods for measuring aquatic primary productivity used for satellite validation and model synthesis. A workshop to commence the working group efforts was held December 05-07, 2018 at the University Space Research Association headquarters in Columbia, MD U.S.A., bringing together 26 active researchers from 16 institutions. The group discussed the primary differences, nuances, scales, uncertainties, definitions, and best practices for measurements of primary productivity derived from in situ/on-deck/laboratory radio/stable isotope incubations, dissolved oxygen concentrations (from incubations or autonomous platforms such as floats or gliders), oxygen-argon ratios, triple oxygen isotope, natural fluorescence, and FRRF/ETR/kinetic analysis. These discussions highlighted the necessity to move the community forward towards the establishment of climate-quality primary productivity measurements that follow uniform protocols, which is imperative to ensure that existing and future measurements can be compared, assimilated, and their uncertainties determined for model development and validation. The specific deliverable resulting from of this activity will be a protocol document, published in coordination with the IOCCG. This presentation will discuss the findings of the meeting, and address future activities of the working group.

Vandermeulen, Ryan A.↗

NASA NTRS2020

TRAPPIST-1 Habitable Atmosphere Intercomparison (THAI): motivations and protocol version 1.0

Upcoming telescopes such as the James Webb Space Telescope (JWST), the European Extremely Large Telescope (E-ELT), the Thirty Meter Telescope (TMT) or the Giant Magellan Telescope (GMT) may soon be able to characterize, through transmission, emission or reflection spectroscopy, the atmospheres of rocky exoplanets orbiting nearby M dwarfs. One of the most promising candidates is the late M-dwarf system TRAPPIST-1, which has seven known transiting planets for which transit timing variation (TTV) measurements suggest that they are terrestrial in nature, with a possible enrichment in volatiles. Among these seven planets, TRAPPIST-1e seems to be the most promising candidate to have habitable surface conditions, receiving ∼66 % of the Earth's incident radiation and thus needing only modest greenhouse gas inventories to raise surface temperatures to allow surface liquid water to exist. TRAPPIST-1e is, therefore, one of the prime targets for the JWST atmospheric characterization. In this context, the modeling of its potential atmosphere is an essential step prior to observation. Global climate models (GCMs) offer the most detailed way to simulate planetary atmospheres. However, intrinsic differences exist between GCMs which can lead to different climate prediction and thus observability of gas and/or cloud features in transmission and thermal emission spectra. Such differences should preferably be known prior to observations. In this paper we present a protocol to intercompare planetary GCMs. Four testing cases are considered for TRAPPIST-1e, but the methodology is applicable to other rocky exoplanets in the habitable zone. The four test cases included two land planets composed of modern-Earth and pure-CO2 atmospheres and two aqua planets with the same atmospheric compositions. Currently, there are four participating models (LMDG, ROCKE-3D, ExoCAM, UM); however, this protocol is intended to let other teams participate as well.

Thomas Jean Daniel Fauchez↗

NASA NTRS2021

Bhutan Agriculture: Developing a Crop Mask for Rice and Creating a Data Collection Protocol Utilizing Remotely Sensed Data in Bhutan

Rice cultivation in Bhutan has been increasingly threatened by deteriorating soil health and outbreaks of diseases and pests associated with the global change in climate patterns. Field surveys, which the national government of Bhutan has relied on to monitor remote agricultural lands, are becoming increasingly overwhelmed by growing threats to agricultural health. To address these concerns, NASA DEVELOP partnered with the Department of Agriculture of Bhutan, the Bhutan Foundation, and the Ugyen Wangchuck Institute of Conservation and Environmental Research (UWICER) and worked to increase the government of Bhutan’s agricultural monitoring capacity. Utilizing Earth observations including Landsat 8 Operational Land Imager (OLI), Sentinel-1 C-band Synthetic Aperture Radar (C-SAR), Shuttle Radar Topography Mission (SRTM), and Planet imagery, the DEVELOP team worked with NASA SERVIR and created a sampling protocol to identify rice plantations and supplement field surveys for more efficient agriculture monitoring. The analysis focused on districts Paro, Punakha, Samtse, Sarpang, Trongsa, Zhemgang, Wangdue Phodrang, and Samdrup Jongkhar in the year 2020 during the period of transplantation (June) to harvesting of rice (November). The team provided the partners with a sampling protocol for integrating NASA Earth observations into their crop monitoring methods, as well as a crop mask for rice identification and to aid crop management. The crop mask for rice was developed using the Random Forest (RF) classifier for the eight districts of Bhutan. Visually, the random forest model has proved to be more accurate and precise than the classification and Regression Tree model. Statistically, the Random Forest model was 91.8% accurate in identifying rice in Bhutan.

Yeshey Seldon↗

NASA NTRSDate not supplied

Evaluation of Planetary Extravehicular Activity Prebreathe Protocols using a 56.5 kPa, 34% O2, 66% N2 Saturation Cabin Atmosphere in an 11-day Hypobaric Hypoxia Study

INTRODUCTION: Apollo missions used 100% O2 cabin atmospheres which effectively eliminated the risk of decompression sickness (DCS) during Lunar extravehicular activities (EVAs, ‘spacewalks’); however, this atmosphere presented a flammability risk that is no longer acceptable to NASA. Denitrogenation prebreathe protocols used to mitigate DCS risk for Space Shuttle and International Space Station EVAs are validated for the microgravity environment, but the significantly increased risk of DCS during equivalent ambulatory surface EVAs make these protocols inapplicable to planetary/Lunar missions. A cabin/vehicle “Exploration Atmosphere” of 56.5 kPa (8.2 psia), 34% O2, 66% N2 has been recommended by NASA for future Moon and Mars missions as a compromise that balances subsequent pre-EVA prebreathe duration, hypoxia, and flammability risk, assuming a 29.6 kPa (4.3 psi) spacesuit. Prebreathe validation studies were initiated utilizing a three-story 6m diameter hypobaric chamber at NASA’s Johnson Space Center. Here, we report the results of a 11-day human-in-the-loop system checkout. METHODS: Six volunteers lived in a hyboparic chamber for 11 days with an ‘exploration atmosphere’ of 56.6kPa/34% O2 66% N2. Subjects acclimated to the exploration atmosphere for 48hrs and thereafter participated in five 6-hour simulated EVAs at 34kPa/85% O2 / 15% N2 over the course of 11 days. Prior to each simulated EVA, subjects underwent a 20-minute prebreathe at 85% O2. The EVA simulation was designed to include tasks that are physically and ergonomically representative of future planetary EVAs, proportionate to the subject’s VO2max. Decompression stress was evaluated during the simulated EVA by serial doppler and echocardiographs alternating every 15min, as well as clinical monitoring for DCS signs/symptoms. RESULTS AND DISCUSSION: Venous gas emboli (VGE) were present in 3 of 6 subjects during EVAs, with peak Grade II VGE as evaluated by Doppler and a peak Eftedal-Brubakk score of 5 by cardiac ultrasonography. Two cases of DCS were diagnosed during the 11-day test chamber. No acute hypoxic symptoms were noted. Musculoskeletal and gastrointestinal complaints were noted, likely associated with the exercise load and the food system. Two cases of DCS (8%) does not cross either accept or reject pre-test criterion, so an additional study is planned for 2023 to meet our pre-test thresholds.

Alejandro Garbino↗

NASA NTRS2022

Benefits and Challenges of CCSDS File Delivery Protocol as Applied to Europa Clipper

—This paper describes the use of the Consultative Committee for Space Data Systems (CCSDS) File Delivery Protocol (CFDP) on the Europa Clipper mission for both uplink and downlink of files. It includes an overview of CFDP, the history of why CFDP was chosen, how it benefits mission operations, some of the mission scenarios that stress CFDP, operability aspects, the best practices that Clipper adopted from other missions and some of the technical challenges with implementation, and verification and validation. The benefits to mission operations accrue because CFDP reduces the need for manual management of file transfer, including retransmission of missing data, and deletion of files only after confirmation of receipt by the ground. The challenges occur because CFDP is a round-trip protocol – it requires messages in both directions to complete a file transfer, and because it uses timers to ensure that control messages are resent if needed to prevent transactions from going stale. Any situations where communication is restricted to a single direction, interrupted, reordered, or backlogged can pose a challenge. There are also implementation challenges. Europa Clipper is the first mission at the Jet Propulsion Laboratory (JPL) to adopt class 2, fully acknowledged, CFDP for both uplink and downlink. The implementation needed new software, requirements and operational procedures. The experience of the Applied Physics Laboratory (APL) with CFDP from their previous missions was crucial to success for Europa Clipper. Because CFDP relies on timers and messages travel in both directions, verification and validation (V&V) requires new approaches. For certain scenarios, a live ground system talking to a live flight system with realistic simulated one-way light times, data rates and data outages must be used.

Albers, Joshua↗

NASA NTRS2024

UAM Source Noise Hemisphere Flight Test Measurement Protocol

The overall goal of the NASA Urban Air Mobility Noise Working Group (UNWG) Ground and Flight Test Subgroup (SG2) is to develop a research measurement protocol, or set of guidelines, which can be used to adequately quantify the Urban Air Mobility (UAM) community noise impact via the creation of acoustic profiles that describe the vehicle source emission characteristics. This document lays out a measurement protocol for acquiring data suitable for the development of compact noise hemispheres that characterize the source acoustic emissions from full-scale UAM or Advanced Air Mobility (AAM) vehicles in outdoor flight. It also provides a narrative describing the numerous aspects of outdoor flight test design and execution that will hopefully prove useful to flight test engineers and practitioners.

Acoustics↗

DOE OSTI2026

Sparse Phase Ansatzes for Resource-Efficient Qudit State Preparation via the SNAP-Displacement Protocol

Efficient preparation of nonclassical bosonic states is a central requirement for quantum computing, simulation, and precision metrology. We study resource-efficient quantum state preparation in bosonic qudit systems using the SNAP-displacement (SD) protocol. Existing SD-based approaches typically require a large number of gates and SNAP phases, resulting in complex control pulses with longer ansatz durations and amplified impact of photon-loss and control errors. In this work, we focus on the near- to medium-term regime, in which noisy quantum devices impose trade-offs on the fidelity that can be achieved, which must be taken into account. Specifically, we propose to optimize only a subset of the SNAP phases and introduce three progressively more general sparse ansatzes. To provide fine-grained control and identify the most suitable ansatz for a given target fidelity, we further employ a scalarized multi-objective optimization that trades off fidelity against either the number of phases or the duration of the ansatz. Numerical results for several target states and qudit dimensions up to $d=64$, evaluated through the hypervolume of the Pareto frontiers, show that these sparse ansatzes achieve favorable trade-offs over the fully parameterized SD protocol in both ideal and noisy settings. The advantage is strongest and most consistent when minimizing the number of phases, while improvements in ansatz duration are smaller and more dependent on the target-state family and noise level, suggesting a practical route to more efficient near- and medium-term bosonic state preparation.

Ferrari Dacrema, Maurizio [Milan Polytechnic] (ORC↗

DOE OSTI2025

EnergyPlus-MCP: A model-context-protocol server for ai-driven building energy modeling

Traditional building energy modeling with the EnergyPlus building performance simulation engine requires domain expertise, programming skills, and intensive manual efforts limiting its effective adoption. This paper introduces EnergyPlus-MCP, the first open-source Model Context Protocol (MCP) server specifically designed for EnergyPlus simulation workflows, establishing a new foundational infrastructure for AI-driven building energy modeling. The MCP server implements a layered architecture with 35 specialized tools spanning model management, editing and analysis, HVAC and other systems configuration inspection, and simulation execution, enabling Large Language Models to interact with EnergyPlus through conversational interfaces. The server addresses critical workflow barriers by automating model validation, streamlining energy efficiency measures modification, and providing intelligent output management with interactive visualization. Through practical demonstrations using a multi-zone building retrofit analysis, we show how the EnergyPlus-MCP server significantly reduces manual efforts while maintaining full simulation rigor. By providing accessible natural language interfaces to sophisticated building energy analysis, this approach enables scalable deployment of simulation expertise across public and private organizations, educational institutions, and research teams, fundamentally transforming traditional building energy modeling practices.

AI↗

DOE OSTI2024

Rephasing spectral diffusion in time-bin spin-spin entanglement protocols

Generating high-fidelity spin-spin entanglement is an essential task of quantum repeater networks for the distribution of quantum information across long distances. Solid-state-based spin-photon interfaces are promising candidates to realize nodes of a quantum network, but are often limited by spectral diffusion of the optical transition, which results in phase errors on the entangled states. Here, we introduce a method to correct phase errors from quasi-static frequency fluctuations after the entangled state is generated, by shelving the emitters in the excited state to refocus the unknown phase. For quasi-static frequency fluctuations, the fidelity is determined only by the lifetime of the excited state used for shelving, making it particularly suitable for systems with a long-lived shelving state with correlated spectral diffusion. Such a shelving state may be found in Kramers doublet systems such as rare-earth emitters and color centers in Si or SiC interfaced with nanophotonic cavities with a strongly frequency-dependent Purcell enhancement. Furthermore, the protocol can be used to generate high-fidelity entangled spin pairs without reducing the rate of entanglement generation.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

NASA NTRS2001

Considerations on communications network protocols in deep space

Communications supporting deep space missions impose numerous unique constraints that impact the architectural choices made for cost-effectiveness. We are entering the era where networks that exist in deep space are needed to support planetary exploration. Cost-effective performance will require a balanced integration of applicable widely used standard protocols with new and innovative designs.

network protocols deep space wireless networks↗