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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 307 records · Page 17
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DOE OSTI2024

Intra- and inter-subtype HIV diversity between 1994 and 2018 in southern Uganda: a longitudinal population-based study

There is limited data on human immunodeficiency virus (HIV) evolutionary trends in African populations. We evaluated changes in HIV viral diversity and genetic divergence in southern Uganda over a 24-year period spanning the introduction and scale-up of HIV prevention and treatment programs using HIV sequence and survey data from the Rakai Community Cohort Study, an open longitudinal population-based HIV surveillance cohort. Gag (p24) and env (gp41) HIV data were generated from people living with HIV (PLHIV) in 31 inland semi-urban trading and agrarian communities (1994–2018) and four hyperendemic Lake Victoria fishing communities (2011–2018) under continuous surveillance. HIV subtype was assigned using the Recombination Identification Program with phylogenetic confirmation. Inter-subtype diversity was evaluated using the Shannon diversity index, and intra-subtype diversity with the nucleotide diversity and pairwise TN93 genetic distance. Genetic divergence was measured using root-to-tip distance and pairwise TN93 genetic distance analyses. Demographic history of HIV was inferred using a coalescent-based Bayesian Skygrid model. Evolutionary dynamics were assessed among demographic and behavioral population subgroups, including by migration status. 9931 HIV sequences were available from 4999 PLHIV, including 3060 and 1939 persons residing in inland and fishing communities, respectively. In inland communities, subtype A1 viruses proportionately increased from 14.3% in 1995 to 25.9% in 2017 (P < .001), while those of subtype D declined from 73.2% in 1995 to 28.2% in 2017 (P < .001). The proportion of viruses classified as recombinants significantly increased by nearly four-fold from 12.2% in 1995 to 44.8% in 2017. Inter-subtype HIV diversity has generally increased. While intra-subtype p24 genetic diversity and divergence leveled off after 2014, intra-subtype gp41 diversity, effective population size, and divergence increased through 2017. Intra- and inter-subtype viral diversity increased across all demographic and behavioral population subgroups, including among individuals with no recent migration history or extra-community sexual partners. This study provides insights into population-level HIV evolutionary dynamics following the scale-up of HIV prevention and treatment programs. Continued molecular surveillance may provide a better understanding of the dynamics driving population HIV evolution and yield important insights for epidemic control and vaccine development.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2023

Optimizing invader suppression to restore native species: Leveraging species traits to overcome collateral damage

Strategies to control ongoing biological invasions are often developed by modelling the invasive species' population and aiming to reduce its abundance. However, if the ultimate objective is to protect and restore native species, focussing solely on the invader may not be optimal because it does not account for (i) species interactions that can cause the invader's impacts to depend nonlinearly on its abundance, (ii) collateral damages to native species incurred due to nonspecific removal methods or (iii) native-invader trait differences. Here, to identify an invader suppression strategy that maximizes average native population size, we applied optimal control theory to a two-species model of a native species threatened by an invasive competitor. We examined trade-offs between iterative physical removals that selectively target invaders and intensifiable chemical control that is nonselective but has higher efficacy. We found that while iterative removals were capable of supporting large native populations when applied continuously, cost could be prohibitively high. In contrast, when favourable native-invader trait differences enabled native species to re-establish more quickly than invaders, intensifiable methods could achieve substantial restoration benefits at lower cost by focussing removal effort into periodic, high-efficacy events. In a metapopulation, removals that rotated among spatial patches were optimal when the native species had higher dispersal, whereas synchronous removals were preferred when native recovery was initiated locally and the invader could disperse. For a case study in Hawaiian streams, we compared how effective two alternative methods of removing invasive live-bearing fishes (poeciliids) might be at restoring the endemic freshwater goby Sicyopterus stimpsoni. We found that rotenone (a piscicidal chemical) offered superior benefits when the control budget was small and efficacy was high, but that electrofishing (use of electricity to manually collect target fish) was better with larger budgets and in many lower-efficacy scenarios. Synthesis and applications. Our findings demonstrate that, by accounting for species interactions and collateral damage, invasive species control strategies can be optimized in light of species traits. Choices about the timing, locations and types of removal events present opportunities to increase the efficiency with which invasive species suppression benefits native species.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2023

Detection efficiency of adult Pacific lamprey passage counts at Leaburg Dam and upstream distribution in the McKenzie River (Oregon, USA)

Abstract Adult Pacific lamprey ( Entosphenus tridentatus ) were counted using consistent methodology since 2005 with a video monitoring system as they passed Leaburg Dam (McKenzie River, Oregon, USA) en route to upstream spawning areas. In this study we evaluated the detection efficiency of the video system and upstream distribution of Pacific lamprey using the video counts of lamprey passage (herein, “dam counts”), passive integrated transponder (PIT) tags, and radio telemetry. In 2019–2020 we collected, tagged and tracked 32 adult lamprey (4 from the McKenzie River and 28 that were translocated from Willamette Falls). All fish were tagged and released into the tailrace of Leaburg Dam in June 2019. Oregon Department of Fish and Wildlife employees and volunteers from the local community conducted mobile radiotracking above and below the dam in drift boats (114 mainstem river kilometers) and on foot (several tributaries). The estimated detection efficiency for dam counts was 92% (95% confidence interval: 67%–99%). Fifty percent (16 of 32) of the tagged lamprey passed the dam, including 13 of 28 that were translocated. Thirty‐seven percent (6 of 16) of lamprey that passed Leaburg Dam were detected in a restored reach of the South Fork McKenzie, 32.6 river kilometers upstream of the dam.

Romer, Jeremy D.↗
DOE OSTI2024

Tracking animal movements via collaborative acoustic telemetry networks: Multiscale habitat use, phenology, and management insights

Abstract Estuaries support diverse fish and invertebrate communities, including resident species that rely on estuarine habitats year‐round and transient migratory species. The unique movement patterns of these animals connect habitats within and far beyond the estuary and are integrally linked to fisheries management objectives. With a focus on Chesapeake Bay, this study leveraged data from collaborative acoustic telemetry networks in the northwest Atlantic to assess habitat use and phenology of movements for seven species of fish (cownose rays, dusky sharks, smooth dogfish, alewife, striped bass, common carp, and blue catfish) and one invertebrate (horseshoe crabs). A total of 288 acoustically tagged individuals were detected >3.2 million times (6,743 to 2,095,717 detections per species) on receivers across ~20.5 degrees of latitude spanning the North American Atlantic seaboard from Florida, USA, to New Brunswick, Canada. Common metrics of movement and phenology grouped these species as resident (common carp, blue catfish, horseshoe crabs), primarily resident in estuaries (juvenile striped bass), and coastal migrant (cownose rays, dusky sharks, smooth dogfish, alewife); maximum distance traveled varied by three orders of magnitude among these species. Further analysis of phenology for coastal migrants elucidated the timing and duration of these species' use of Chesapeake Bay. Collectively, movements linked habitats within Chesapeake Bay and connected the estuary to coastal ecosystems both to the north (e.g., alewife) and south (e.g., cownose rays), creating networks of fisheries management jurisdictions that varied in complexity and identified opportunities for enhancement to current management or co‐management of some species. Our results elucidate the importance of estuaries to species with diverse movement behaviors, identify scales and pathways of habitat connectivity via animal movements, and highlight the utility of collaborative acoustic telemetry networks for quantifying movements relevant to both ecological research and fisheries management.

Livernois, Mariah C.↗
DOE OSTI2023

Amoeba species colonizing the gills of rainbow trout ( Oncorhynchus mykiss ) in Swiss aquaculture

Nodular gill disease (NGD) is an infectious condition characterized by proliferative gill lesions leading to respiratory problems, oxygen deficiency and mortality in fish. Globally, NGD primarily impacts freshwater salmonids in intensive aquaculture systems. In recent years, numerous outbreaks of severe gill disease have affected more than half of the larger rainbow trout ( Oncorhynchus mykiss ) farms in Switzerland, mainly during spring and early summer. Mortality has reached up to 50% in cases where no treatment was administered. Freshwater amoeba are the presumed aetiologic agent of NGD. The gross gill score (GS) categorising severity of gill pathology is a valuable first-line diagnostic tool aiding fish farmers in identifying and quantifying amoebic gill disease (AGD) in farmed marine salmonids. In this study, the GS was adapted to the NGD outbreak in farmed trout in Switzerland. In addition to scoring disease severity, gill swabs from NGD-affected rainbow trout were sampled and amoeba were cultured from these swabs. Morphologic and molecular methods identified six amoeba strains: Cochliopodium sp., Naegleria sp., Vannella sp., Ripella sp., Saccamoeba sp. and Mycamoeba sp. However, the importance of the different amoeba species for the onset and progression of NGD still has to be evaluated. This paper presents the first description of NGD with associated amoeba infection in farmed rainbow trout in Switzerland.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2020

Automatic Classification of Biological Targets in a Tidal Channel Using a Multibeam Sonar

Multibeam sonars are widely used for environmental monitoring of fauna at marine renewable energy sites. However, they can rapidly accrue vast volumes of data, which poses a challenge for data processing. Here, using data from a deployment in a tidal channel with peak currents of 1–2 m s –1 , we demonstrate the data-reduction benefits of real-time automatic classification of targets detected and tracked in multibeam sonar data. First, we evaluate classification capabilities for three machine learning algorithms: random forests, support vector machines, and k-nearest neighbors. For each algorithm, a hill-climbing search optimizes a set of hand-engineered attributes that describe tracked targets. Here, the random forest algorithm is found to be most effective—in postprocessing, discriminating between biological and nonbiological targets with a recall rate of 0.97 and a precision of 0.60. In addition, 89% of biological targets are correctly classified as either seals, diving birds, fish schools, or small targets. Model dependence on the volume of training data is evaluated. Second, a real-time implementation of the model is shown to distinguish between biological targets and nonbiological targets with nearly the same performance as in postprocessing. From this, we make general recommendations for implementing real-time classification of biological targets in multibeam sonar data and the transferability of trained models.

16 TIDAL AND WAVE POWER↗
DOE OSTI2024

Multicellular magnetotactic bacteria are genetically heterogeneous consortia with metabolically differentiated cells

Consortia of multicellular magnetotactic bacteria (MMB) are currently the only known example of bacteria without a unicellular stage in their life cycle. Because of their recalcitrance to cultivation, most previous studies of MMB have been limited to microscopic observations. To study the biology of these unique organisms in more detail, we use multiple culture-independent approaches to analyze the genomics and physiology of MMB consortia at single-cell resolution. We separately sequenced the metagenomes of 22 individual MMB consortia, representing 8 new species, and quantified the genetic diversity within each MMB consortium. This revealed that, counter to conventional views, cells within MMB consortia are not clonal. Single consortia metagenomes were then used to reconstruct the species-specific metabolic potential and infer the physiological capabilities of MMB. To validate genomic predictions, we performed stable isotope probing (SIP) experiments and interrogated MMB consortia using fluorescence in situ hybridization (FISH) combined with nanoscale secondary ion mass spectrometry (NanoSIMS). By coupling FISH with bioorthogonal noncanonical amino acid tagging (BONCAT), we explored their in situ activity as well as variation of protein synthesis within cells. We demonstrate that MMB consortia are mixotrophic sulfate reducers and that they exhibit metabolic differentiation between individual cells, suggesting that MMB consortia are more complex than previously thought. These findings expand our understanding of MMB diversity, ecology, genomics, and physiology, as well as offer insights into the mechanisms underpinning the multicellular nature of their unique lifestyle.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2020

Meiotic susceptibility for induction of sperm with chromosomal aberrations in patients receiving combination chemotherapy for Hodgkin lymphoma

Improvements in survival rates with gonad-sparing protocols for childhood and adolescence cancer have increased the optimism of survivors to become parents after treatment. Findings in rodents indicate that chromosomal aberrations can be induced in male germ cells by genotoxic exposures and transmitted to offspring and future generations with effects on development, fertility and health. Thus, there is a need for effective technologies to identify human sperm carrying chromosomal aberrations to assess the germ-line risks, especially for cancer survivors who have received genotoxic therapies. The time-dependent changes in the burden of sperm carrying structural chromosomal aberrations were assessed for the first time in a cancer setting, using the AM8 sperm FISH protocol which simultaneously detects abnormalities in chromosomal structure and number in sperm. Nine Hodgkin lymphoma (HL) patients provided 20 semen samples before, during, and after NOVP therapy (Novantrone, Oncovin, Velban and Prednisone) and radiation therapy that produced scattered gonadal doses from <0.05 to 0.6 Gy. Late meiosis was found to be the most sensitive to NOVP treatment for the production of sperm with chromosomal abnormalities, both in structure and number. Earlier stages of spermatogenesis were less sensitive and there was no evidence that therapy-exposed stem cells resulted in increased frequencies of sperm with abnormalities in chromosomal structure or number. This indicates that NOVP therapy may increase the risks for paternal transmission of chromosomal structural aberrations for sperm produced 32 to 45 days after a treatment with these drugs and implies that there are no excess risks for pregnancies conceived more than 6 months after this therapy. This clinical evaluation of the AM8 sperm FISH protocol indicates that it is a promising tool for assessing an individual’s burden of sperm carrying chromosomal structural aberrations as well as aneuploidies after cancer therapy, with broad applications in other clinical and environmental situations that may pose aneugenic or clastogenic risks to human spermatogenesis.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2023

A correlation between seasonally changing photoperiod, whole body lipid, and condition factor in juvenile spring Chinook salmon (Oncorhynchus tshawytscha)

The regulation of lipid stores is a central process for the physiology and ecology of fishes. Seasonal variation in lipid stores has been directly linked to survival of fishes across periods of food deprivation. We assessed whether a seasonally changing photoperiod was correlated to seasonal changes in energetic status to help better understand these important processes. Groups of first feeding Chinook salmon fry were introduced to a seasonal photoperiod cycle, but the point of entrance into the seasonal cycle varied from near the winter solstice (December), to either side of the spring equinox (February & May). Temperature and feeding rate were similar for all treatments. Subsequently, condition factor and whole body lipid content were assessed through a seasonal progression. Throughout most of the experiment, length and weight did not differ between the different photoperiod treatments, however whole body lipid and Fulton’s condition factor did. Furthermore, changes in both whole body lipid and Fulton’s condition factor in all treatment groups followed a similar seasonal pattern that was inversely related to day length (highest K and lipid levels found during days with the least light). These results suggest that regardless of age or size, there is a correlation between seasonal changes in photoperiod and changes in body composition in juvenile Chinook salmonids.

Spangenberg, Dina K. (ORCID:000000018861395X)↗
DOE OSTI2025

Age at release affects developmental physiology and sex-specific phenotypic diversity of hatchery steelhead trout (Oncorhynchus mykiss)

Most steelhead trout hatcheries increase growth rate during rearing to produce and release yearling smolts for harvest augmentation, but natural steelhead exhibit variable age of smoltification, so this common rearing practice may not be ideal for programs focused on recovering imperiled wild stocks; therefore, it is important to investigate and compare alternative hatchery rearing methods that promote life history diversity. Over six consecutive years, the Winthrop National Fish Hatchery on the Methow River, WA reared and released paired groups of age-1 (S1) and age-2 (S2) steelhead smolts. To understand how the two rearing methods affected developmental ontogeny and life-history, fish were sampled prior to hatchery release for factors associated with smoltification (size, gill Na+/K+ ATPase activity, and a qualitative smolt phenotype) and sexual maturation (sex, pituitary and testis mRNA transcripts, gonadosomatic index, and plasma 11-ketotestosterone). Our objectives were to quantify levels of smoltification and male maturation during hatchery rearing, combine metrics to estimate residualism (failure to migrate upon release), and compare the treatments by sex. Overall, S2 rearing produced 7.8% more smolts and 44-fold (4.4 vs. 0.1%) more precociously mature males than S1 rearing. Conversely, S1 rearing produced 31.6% more residuals than S2 rearing. While the proportion of total male residuals was comparable between treatments, the S1 treatment produced approximately five-fold more female residuals (20.6 vs. 4.2%). Because residuals contribute minimally to adult returns and the number of returning adult females is critical to the success of salmonid supplementation efforts, developing rearing techniques that maximize migration in females is a management priority. Physiological assessments are useful for characterizing and quantifying the effects and risks of different hatchery rearing regimes on steelhead life-history, in addition to providing sex-specific guidance to inform and optimize conservation management goals in supplementation programs.

Middleton, Mollie A. (ORCID:0009000905577865)↗
DOE OSTI2021

RACEE Energy Efficiency Implementation for Holy Cross, Alaska (Final Report)

The Deg Hit’an Athabascan village of Holy Cross is located along the Ghost Creek Slough bank of the Yukon River. Holy Cross is home to almost 170 people who are accustomed to both the advantages and disadvantages of living off the road system - the nearest major road is some 300 miles to the east. On one hand, Holy Cross residents have greater access to practicing traditional activities like subsistence hunting and fishing. Families in the area have fished, hunted, and gathered food together for many generations and the land is imbued with this special history. But compared to Alaskans living on the road system, the residents of Holy Cross pay higher prices for basic necessities since goods are transported via air service and seasonal barge shipments. Holy Cross residents also accumulate higher energy costs, as diesel fuel is the primary fuel source for heating and lighting and costs over $\$6$ per gallon. Affording these high living costs becomes a barrier for Holy Cross residents who want to stay in the village or participate in subsistence activities requiring transportation by boat or ATV.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗
DOE OSTI2022

Designing Hydropower Flows to Balance Energy and Environmental Needs (HydroWIRES Topic A Final Project Report)

Hydropower is expected to play a new role in the US electricity grid as more variable renewable energy sources like wind and solar come online. Wind and solar generation increase fluctuations in electrical supply increasing the value of flexible generation sources that can quickly ramp generation up and down. The flexible generation hydropower can provide as well as the ancillary services (e.g., frequency and voltage regulation and reserves, black start capability) it provides for balancing and stabilizing the power grid are predicted to be of increased value in these future grid scenarios. Yet, the flexibility of hydropower may come with environmental costs due to up- and down-ramping of hydropower plants (i.e., quickly increasing or decreasing generation flows, respectively) which may strand fish, dewater or scour fish nests, alter habitat, or create unsafe recreational conditions that may be unacceptable to participants in the hydropower regulatory process. These types of environmental impacts are often mitigated through environmental flow requirements that specify minimum or maximum flow releases, or ramp-rates changes allowed at a hydropower facility. While it is not currently known to what degree electrical grid reliability could be affected by environmental flow requirements, gaining a better understanding of these interactions before the grid becomes more deeply decarbonized can help define what policy, regulation, or infrastructure may be needed to support the clean energy transition. As the future grid will rely on hydropower to provide both flexibility and robust environmental protections, the analyses and tools described in this report are centered on making mechanistic linkages between energy and the environment in hydropower systems. This understanding of energy-environment linkages can provide the foundational understanding needed to quantitatively assess the trade-offs between the increased generation flexibility that hydropower will be expected to provide and the environmental impacts of this flexibility. This report seeks to provide an objective foundation for building future science and tools that can be used by a broad spectrum of the hydropower community that is involved in licensing or environmental regulatory proceedings tasked with balancing energy and environmental objectives through flow management.

13 HYDRO ENERGY↗
DOE OSTI2023

East Tennessee Technology Park Biological Monitoring and Abatement Program (2023 Fiscal Year Report)

In FY 2023, the East Tennessee Technology Park’s (ETTP’s) Biological Monitoring and Abatement Program (BMAP) consisted of two tasks that reflected different but complementary approaches to evaluating the effects of ETTP effluents on the ecological integrity of waters near ETTP. These tasks included (1) fish and clams bioaccumulation monitoring and (2) fish and benthic macroinvertebrate species richness and density monitoring. The sampling and analysis requirements for the ETTP BMAP in FY 2023 are outlined in the sampling and analysis plan (UCOR 2023).

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
DOE OSTI2023

Energy Transitions Initiative Partnership Project: Alaska Longline Fishermen’s Association Alternative Fuels Review

This report provides information about additional options in electrifying the Alaska Longline Fishermen’s Association (ALFA) fishing fleet. Currently, the Energy Transitions Initiative Partnership Project is prioritizing electric hybrid and battery electric options for retrofit of select fishing vessels in the fleet. However, there are other options for reducing carbon emissions among the fleet, namely hydrogen, ammonia, and biofuels. The use of these alternative fuels may be an option for the fleet in the future as ALFA addresses its vulnerabilities. A literature review of available resources on these fuels was conducted to provide information on several topics associated with implementation of these technologies, including current applications, commercially available power modules, necessary components, infrastructure, and safety codes and standards. An additional aspect that this report investigates is general sizing and refueling schedule based on operating records of multiple ships in the fleet.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗
DOE OSTI2023

Energy Transitions Initiative Partnership Project: Alaska Longline Fishermen’s Association Alternative Fuels Review

This report provides information about additional options in electrifying the Alaska Longline Fishermen’s Association (ALFA) fishing fleet. Currently, the Energy Transitions Initiative Partnership Project is prioritizing electric hybrid and battery electric options for retrofit of select fishing vessels in the fleet. However, there are other options for reducing carbon emissions among the fleet, namely hydrogen, ammonia, and biofuels. The use of these alternative fuels may be an option for the fleet in the future as ALFA addresses its vulnerabilities. A literature review of available resources on these fuels was conducted to provide information on several topics associated with implementation of these technologies, including current applications, commercially available power modules, necessary components, infrastructure, and safety codes and standards. An additional aspect that this report investigates is general sizing and refueling schedule based on operating records of multiple ships in the fleet.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗
DOE OSTI2024

East Tennessee Technology Park Biological Monitoring and Abatement Program - 2024 Fiscal Year Report

In FY 2024, the East Tennessee Technology Park’s (ETTP’s) Biological Monitoring and Abatement Program (BMAP) consisted of two tasks that reflect different but complementary approaches to evaluating the effects of ETTP effluents on the ecological integrity of waters near ETTP. These tasks included (1) fish and clam bioaccumulation monitoring and (2) fish and benthic macroinvertebrate species richness and density monitoring. The sampling and analysis requirements for the ETTP BMAP in FY 2024 are outlined in the sampling and analysis plan (UCOR 2023).

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

Bioaccumulation Factor for Radium in Rio Grande Biota

The Los Alamos National Laboratory (LANL or Laboratory) Environmental Protection and Compliance Division’s Environmental Stewardship Group (EPC-ES) performs the radiological analysis portion of biota dose assessments using the computer program RESRAD-Biota (DOE 2004, DOE 2020). RESRAD-Biota uses a default bioaccumulation factor, B iv , for radium in aquatic animals. This bioaccumulation factor, also listed in Department of Energy (DOE) Standard, DOE-STD-1153-2019, is the ratio of a radionuclide concentration in fresh weight biota to the environment in which it resides, which for aquatic animals is water. DOE-STD-1153-2019 offers conservative values for these ratios that are specific to the radiological contaminant, the biota, and the environment. The B iv in RESRAD-Biota can be used for general screening (Level 1 screening). If the factor proves to be too conservative, then a site-specific B iv may be used. The biota dose assessment of fish from the northern New Mexico region of the Rio Grande fail the general screen, which indicates that further analysis is necessary. Level 2 analysis is site-specific but still has conservative limitations. Level 3 analysis is a more realistic and representative, sitespecific analysis. The purpose of this paper is to use data specific to the Rio Grande to calculate a new aquatic animal radium B iv . This B iv can then be used for Level 3 analysis of aquatic animals specifically in the northern New Mexico region of the Rio Grande. The calculated radium B iv is a useful value for performing detailed, accurate biota dose assessments and calculating dose to fish using RESRAD-Biota when only sediment data are available.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

East Tennessee Technology Park Biological Monitoring and Abatement Program—2025 Fiscal Year Report

In FY 2025, the East Tennessee Technology Park’s (ETTP’s) Biological Monitoring and Abatement Program (BMAP) consisted of two tasks that reflect different but complementary approaches to evaluating the effects of ETTP effluents on the ecological integrity of waters near ETTP. These tasks included (1) fish and clam bioaccumulation monitoring and (2) fish and benthic macroinvertebrate species richness and density monitoring. The sampling and analysis requirements for the ETTP BMAP in FY 2025 are outlined in the sampling and analysis plan (UCOR 2024). Major sampling sites for the BMAP are shown in Figure 1 and Figure 2.

54 ENVIRONMENTAL SCIENCES↗