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At least 307 records · Page 17

Cryo-electron tomography related radiation-damage parameters for individual-molecule 3D structure determination

To understand the dynamic structure–function relationship of soft- and biomolecules, the determination of the three-dimensional (3D) structure of each individual molecule (nonaveraged structure) in its native state is sought-after. Cryo-electron tomography (cryo-ET) is a unique tool for imaging an individual object from a series of tilted views. However, due to radiation damage from the incident electron beam, the tolerable electron dose limits image contrast and the signal-to-noise ratio (SNR) of the data, preventing the 3D structure determination of individual molecules, especially at high-resolution. Although recently developed technologies and techniques, such as the direct electron detector, phase plate, and computational algorithms, can partially improve image contrast/SNR at the same electron dose, the high-resolution structure, such as tertiary structure of individual molecules, has not yet been resolved. Here, we review the cryo-electron microscopy (cryo-EM) and cryo-ET experimental parameters to discuss how these parameters affect the extent of radiation damage. This discussion can guide us in optimizing the experimental strategy to increase the imaging dose or improve image SNR without increasing the radiation damage. With a higher dose, a higher image contrast/SNR can be achieved, which is crucial for individual-molecule 3D structure. With 3D structures determined from an ensemble of individual molecules in different conformations, the molecular mechanism through their biochemical reactions, such as self-folding or synthesis, can be elucidated in a straightforward manner.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Monte Carlo Simulation and Reconstruction: Assessment of Myocardial Perfusion Imaging of Tracer Dynamics With Cardiac Motion Due to Deformation and Respiration Using Gamma Camera With Continuous Acquisition

Purpose: Myocardial perfusion imaging (MPI) with single photon emission computed tomography (SPECT) is routinely used for stress testing in nuclear medicine. Recently, our group extended its potential going from 3D visual qualitative image analysis to 4D spatiotemporal reconstruction of dynamically acquired data to capture the time variation of the radiotracer concentration and the estimated myocardial blood flow (MBF) and coronary flow reserve (CFR). However, the quality of reconstructed image is compromised due to cardiac deformation and respiration. The work presented here develops an algorithm that reconstructs the dynamic sequence of separate respiratory and cardiac phases and evaluates the algorithm with data simulated with a Monte Carlo simulation for the continuous image acquisition and processing with a slowly rotating SPECT camera. Methods: A clinically realistic Monte Carlo (MC) simulation is developed using the 4D Extended Cardiac Torso (XCAT) digital phantom with respiratory and cardiac motion to model continuous data acquisition of dynamic cardiac SPECT with slowly rotating gamma cameras by incorporating deformation and displacement of the myocardium due to cardiac and respiratory motion. We extended our previously developed 4D maximum-likelihood expectation-maximization (MLEM) reconstruction algorithm for a data set binned from a continuous list mode (LM) simulation with cardiac and respiratory information. Our spatiotemporal image reconstruction uses splines to explicitly model the temporal change of the tracer for each cardiac and respiratory gate that delineates the myocardial spatial position as the tracer washes in and out. Unlike in a fully list-mode data acquisition and reconstruction the accumulated photons are binned over a specific but very short time interval corresponding to each cardiac and respiratory gate. Reconstruction results are presented showing the dynamics of the tracer in the myocardium as it continuously deforms. These results are then compared with the conventional 4D spatiotemporal reconstruction method that models only the temporal changes of the tracer activity. Mean Stabilized Activity (MSA), signal to noise ratio (SNR) and Bias for the myocardium activities for three different target-to-background ratios (TBRs) are evaluated. Dynamic quantitative indices such as wash-in (K1) and wash-out (k2) rates at each gate were also estimated. Results: The MSA and SNR are higher with higher TBRs while biases were improved with higher TBRs to less than 10%. The correlation between exhalation-inhalation sequence with the ground truth during respiratory cycle was excellent. Our reconstruction method showed better resolved myocardial walls during diastole to systole as compared to the ungated 4D image. Estimated values of K1 and k2 were also consistent with the ground truth. Conclusion: The continuous image acquisition for dynamic scan using conventional two-head gamma cameras can provide valuable information for MPI. Our study demonstrated the viability of using a continuous image acquisition method on a widely used clinical two-head SPECT system. Our reconstruction method showed better resolved myocardial walls during diastole to systole as compared to the ungated 4D image. Precise implementation of reconstruction algorithms, better segmentation techniques by generating images of different tissue types and background activity would improve the feasibility of the method in real clinical environment.

60 APPLIED LIFE SCIENCES↗

Quantized Constant-Q Gabor Atoms for Sparse Binary Representations of Cyber-Physical Signatures

Increased data acquisition by uncalibrated, heterogeneous digital sensor systems such as smartphones present new challenges. Binary metrics are proposed for the quantification of cyber-physical signal characteristics and features, and a standardized constant-Q variation of the Gabor atom is developed for use with wavelet transforms. Two different continuous wavelet transform (CWT) reconstruction formulas are presented and tested under different signal to noise ratio (SNR) conditions. A sparse superposition of Nth order Gabor atoms worked well against a synthetic blast transient using the wavelet entropy and an entropy-like parametrization of the SNR as the CWT coefficient-weighting functions. The proposed methods should be well suited for sparse feature extraction and dictionary-based machine learning across multiple sensor modalities.

46 - INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AN↗

Anomaly Detection in Liquid Sodium Cold Trap Operation with Multisensory Data Fusion Using Long Short-Term Memory Autoencoder

Sodium-cooled fast reactors (SFR), which use high temperature fluid near ambient pressure as coolant, are one of the most promising types of GEN IV reactors. One of the unique challenges of SFR operation is purification of high temperature liquid sodium with a cold trap to prevent corrosion and obstructing small orifices. We have developed a deep learning long short-term memory (LSTM) autoencoder for continuous monitoring of a cold trap and detection of operational anomaly. Transient data were obtained from the Mechanisms Engineering Test Loop (METL) liquid sodium facility at Argonne National Laboratory. The cold trap purification at METL is monitored with 31 variables, which are sensors measuring fluid temperatures, pressures and flow rates, and controller signals. Loss-of-coolant type anomaly in the cold trap operation was generated by temporarily choking one of the blowers, which resulted in temperature and flow rate spikes. The input layer of the autoencoder consisted of all the variables involved in monitoring the cold trap. The LSTM autoencoder was trained on the data corresponding to cold trap startup and normal operation regime, with the loss function calculated as the mean absolute error (MAE). The loss during training was determined to follow log-normal density distribution. During monitoring, we investigated a performance of the LSTM autoencoder for different loss threshold values, set at a progressively increasing number of standard deviations from the mean. The anomaly signal in the data was gradually attenuated, while preserving the noise of the original time series, so that the signal-to-noise ratio (SNR) averaged across all sensors decreased below unity. Results demonstrate detection of anomalies with sensor-averaged SNR < 1.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

How Much Attenuation Extinguishes mm-Wave Vertically Pointing Radar Return Signals?

Vertically pointing radars (VPRs) operating at millimeter wavelengths measure the power return from raindrops enabling precipitation retrievals as a function of height. However, as the rain rate increases, there are combinations of rain rate and rain path length that produce sufficient attenuation to prevent the radar from detecting raindrops all the way through rain shafts. This study explores the question: Which rain rate and path length combinations completely extinguish radar return signals for VPRs operating between 3 and 200 GHz? An important step in these simulations is converting attenuated radar reflectivity factor into radar received signal-to-noise ratio (SNR) in order to determine the range where the SNR drops below the receiver detection threshold. Configuring the simulations to mimic a U.S. Department of Energy Atmospheric Radiation Mission (ARM) W-band (95 GHz) radar deployed in Brazil, the simulation results indicate that a W-band radar could observe raindrops above 3.5 km only when the rain rate was less than approximately 4 mm h -1 . The deployed W-band radar measurements confirm the simulation results with maximum observed heights ranging between 3 and 4.5 km when a surface disdrometer measured 4 mm h -1 rain rate (based on 25-to-75 percentiles from over 25,000 W-band radar profiles). In summary, this study contributes to our understanding of how rain and atmospheric gas attenuation impacts the performance of millimeter-wave VPRs and will help with the design and configuration of multi-frequency VPRs deployed in future field campaigns.

54 ENVIRONMENTAL SCIENCES↗

A Multiwavelength Investigation of PSR J2229+6114 and its Pulsar Wind Nebula in the Radio, X-Ray, and Gamma-Ray Bands

Abstract G106.3+2.7, commonly considered to be a composite supernova remnant (SNR), is characterized by a boomerang-shaped pulsar wind nebula (PWN) and two distinct (“head” and “tail”) regions in the radio band. A discovery of very-high-energy gamma-ray emission (E γ > 100 GeV) followed by the recent detection of ultrahigh-energy gamma-ray emission (E γ > 100 TeV) from the tail region suggests that G106.3+2.7 is a PeVatron candidate. We present a comprehensive multiwavelength study of the Boomerang PWN (100″ around PSR J2229+6114) using archival radio and Chandra data obtained two decades ago, a new NuSTAR X-ray observation from 2020, and upper limits on gamma-ray fluxes obtained by Fermi-LAT and VERITAS observatories. The NuSTAR observation allowed us to detect a 51.67 ms spin period from the pulsar PSR J2229+6114 and the PWN emission characterized by a power-law model with Γ = 1.52 ± 0.06 up to 20 keV. Contrary to the previous radio study by Kothes et al., we prefer a much lower PWNB-field (B∼ 3μG) and larger distance (d∼ 8 kpc) based on (1) the nonvarying X-ray flux over the last two decades, (2) the energy-dependent X-ray size of the PWN resulting from synchrotron burn-off, and (3) the multiwavelength spectral energy distribution (SED) data. Our SED model suggests that the PWN is currently re-expanding after being compressed by the SNR reverse shock ∼1000 yr ago. In this case, the head region should be formed by GeV–TeV electrons injected earlier by the pulsar propagating into the low-density environment.

Astronomy & Astrophysics↗

Gravitational-wave and Gravitational-wave Memory Signatures of Core-collapse Supernovae

Abstract In this paper, we calculate the energy, signal-to-noise ratio (SNR), detection range, and angular anisotropy of the matter, matter memory, and neutrino memory gravitational-wave (GW) signatures of 21 three-dimensional initially nonrotating core-collapse supernova (CCSN) models carried to late times. We find that inferred energy, SNR, and detection range are angle-dependent quantities, and that the spread of possible energy, signal to noise, and detection ranges across all viewing angles generally increases with progenitor mass. When examining the low-frequency matter memory and neutrino memory components of the signal, we find that the neutrino memory is the most detectable component of a CCSN GW signal, and that DECIGO is best equipped to detect both matter memory and neutrino memory. Moreover, we find that the polarization angle between the h + and h × strains serves as a unique identifier of matter and neutrino memory. Finally, we develop a Galactic density- and stellar mass-weighted formalism to calculate the rate at which we can expect to detect CCSN GW signals with the Advanced Laser Interferometer Gravitational-Wave Observatory (aLIGO). When considering only the matter component of the signal, the aLIGO detection rate is around 65% of the total Galactic supernova rate, but increases to 90% when incorporating the neutrino memory component. We find that all future detectors (Einstein Telescope, Cosmic Explorer, DECIGO) will be able to detect CCSN GW signals from the entire Galaxy, and for the higher-mass progenitors even into the Local Group of galaxies.

Choi, Lyla (ORCID:0000000153860133)↗

The Roman View of Strong Gravitational Lenses

Galaxy–galaxy strong gravitational lenses can constrain dark matter models and the Lambda cold dark matter cosmological paradigm at subgalactic scales. Currently, there is a dearth of images of these rare systems with high signal-to-noise ratio (SNR) and angular resolution. The Nancy Grace Roman Space Telescope (hereafter Roman), scheduled for launch in late 2026, will play a transformative role in strong-lensing science with its planned wide-field surveys. With its remarkable 0.281 square degree field of view and diffraction-limited angular resolution of ~0$^{''}_.$1, Roman is uniquely suited to characterizing dark matter substructure from a robust population of strong lenses. We present a yield simulation of detectable strong lenses in Roman’s planned High Latitude Wide Area Survey (HLWAS). We simulate a population of galaxy–galaxy strong lenses across cosmic time with cold dark matter subhalo populations, select those detectable in the HLWAS, and generate simulated images accounting for realistic Wide Field Instrument detector effects. For a fiducial case of single 146 s exposures, we predict around 160,000 detectable strong lenses in the HLWAS, of which about 500 will have sufficient SNR to be amenable to detailed substructure characterization. We investigate the effect of variation of the point-spread function across Roman’s field of view on detecting individual subhalos and the suppression of the subhalo mass function at low masses. Our simulation products are available to support strong-lens science with Roman, such as training neural networks and validating dark matter substructure analysis pipelines.

79 ASTRONOMY AND ASTROPHYSICS↗

Calibrating radar wind profiler reflectivity factor using surface disdrometer observations

Abstract. This study uses surface disdrometer reflectivity factor estimates to calibrate the vertical and off-vertical pointing radar beams produced by an ultra high frequency (UHF) band radar wind profiler (RWP) deployed at the US Department of Energy (DOE) Atmospheric Radiation Measurement (ARM) program Southern Great Plains (SGP) Central Facility in northern Oklahoma from April 2011 through July 2019. The methodology consists of five steps. First, the recorded Doppler velocity power spectra are adjusted to account for Nyquist velocity aliasing and coherent integration filtering effects. Second, the spectrum moments are calculated. The third step increases the signal-to-noise ratio (SNR) due to inflated noise power estimates during convective rain events that cause SNR to be biased low. The fourth step determines the RWP calibration constant for one radar beam (called the “reference” beam) by comparing uncalibrated RWP reflectivity factors at 500 m above the ground to 1 min resolution surface disdrometer reflectivity factors. The last step uses the calibrated reference beam reflectivity factor to calibrate the other radar beams during precipitation. There are two key findings. The RWP sensitivity decreased by approximately 3 to 4 dB yr−1 as the hardware aged. This drift was slow enough that the reference calibration constant can be estimated over 3-month intervals using episodic rain events. The calibrated moments are available on the DOE ARM data archive, and the Python processing code is available on public repositories.

54 ENVIRONMENTAL SCIENCES↗

Wind and Temperature Consensus at Horn Point, HU-Beltsville, Piney Run (Maryland) in support of CoURAGE

The Maryland Department of the Environment (MDE) operates a ground-based atmospheric profiling network consisting of collocated radar wind profilers (RWP) and radio acoustic sounding systems (RASS) as part of its Ambient Air Monitoring Program. This network provides continuous observations of wind and temperature structure in the lower troposphere to support air quality forecasting, regulatory analysis, and atmospheric research. The network currently includes three fixed sites across Maryland: Horn Point (HP, lower eastern shore) [38.587525°,-76.141006°], Howard University-Beltsville (HUB, central Maryland) [39.055277°, -76.878632°], and Piney Run (PR, western Maryland) [39.705950°, -79.012000°] The network is designed to capture regional variability in atmospheric transport and boundary-layer processes. These systems measure vertical profiles of horizontal wind speed and direction using Doppler radar techniques, with observations typically spanning from ~100 m above ground level up to approximately 2.5–4 km. Measurements are derived from the Doppler shift of backscattered electromagnetic signals, enabling retrieval of wind vectors at multiple altitudes with high temporal resolution (e.g., 30-minute averages reported every 6 minutes). Each radar wind profiler is paired with a Radio Acoustic Sounding System (RASS) to provide profiles of virtual temperature in the lower atmosphere (~100–200 m AGL) by measuring the propagation speed of acoustic waves. Together, the RWP/RASS system yields a coupled data set of thermodynamic and kinematic atmospheric structure, including additional parameters such as vertical velocity, radial velocity, signal-to-noise ratio, and spectral width for advanced analysis. There are two types of files for each station: wind data (files with a "w" prefix) and virtual temperature RASS data (files with a "t" prefix). The wind data files are in the format wYYDDD.cns, where YY is the 2-digit year and DDD is the day of the year. The RASS virtual temperature data files are in the format tYYDDD.cns. Each record has the following header structure: Line 1 : Station Name RASS files Line 2 : RASS rev DeTect_2.0, WINDS files Line 2 : WINDS rev ATI 5.1 Line 3 : N latitude, W longitude, and site elevation (m) Line 4 : Date and begin time of consensus: yy mm dd hh mn ss plus # minutes to add to get UTC Line 5 : Consensus averaging time (minutes); number of beams; number of range gates Line 6 : Number of records required to make consensus (num) total number of records (tot) and the consensus window size (m/s) in the format: num:tot (window) RASS files Line 7 : no. of coded cells, no. of spec, pulse width (ns), and inter-pulse period (µs), WINDS files Line 7 : No. of coded cells, no. of spectra, pulse width (ns), and inter-pulse period (µs), each with a pair of values: first value is for oblique beams, second for vertical RASS files Line 8 : Full scale Doppler value (m/s) Delay to first gate (ns) Number of gates Spacing of gates (ns), WINDS files Line 8 : Full scale Doppler velocity (m/s), oblique and vertical Vertical correction applied to oblique beams? (0 = no, 1 = yes) Delay to first gate (ns), oblique and vertical Number of gates, oblique and vertical Spacing of gates (ns), oblique and vertical Line 9 : Azimuth and elevation (9s indicate vertical beam not used) RASS files Line 10, values : HT = Height above ground (km), T = Uncorrected virtual temperature consensus (deg C), Tc = Corrected virtual temperature consensus (deg C), W = Vertical wind consensus (9s indicate vertical beam not used, w-component, positive upward, m/s), CNT = Number of records that made consensus (for the 3 values in same order), SNR = Average signal to noise ratio (dB) of records in consensus (same order) WINDS files Line 10, values : HT = Height above ground (km), SPD = Wind speed (m/s), DIR = Wind direction (deg E of N from N), RAD = Radial velocities for each beam (m/s) in order given in azimuth and elevation line (positive toward radar; 9s indicate vertical beam not used, CNT = Number of records that made consensus, SNR = Average signal to noise ratio (dB) of records in consensus

{"wind speed and direction",temperature}↗

Delta modulation

The conclusions of the design research of the song adaptive delta modulator are presented for source encoding voice signals. The variation of output SNR vs input signal power/when 8, 9, and 10 bit internal arithmetic is employed. Voice intelligibility tapes to test the 10-bit system are used. An analysis of a delta modulator is also presented designed to minimize the in-band rms error. This is accomplished by frequency shaping the error signal in the modulator prior to hard limiting. The result is a significant increase in the output SNR measured after low pass filtering.

Schilling, D. L.↗

Simplified formula for mean cycle-slip time of phase-locked loops with steady-state phase error.

Previous work shows that the mean time from lock to a slipped cycle of a phase-locked loop is given by a certain double integral. Accurate numerical evaluation of this formula for the second-order loop is extremely vexing because the difference between exponentially large quantities is involved. The presented article demonstrates a method in which a much-reduced precision program can be used to obtain the mean first-cycle slip time for a loop of arbitrary degree tracking at a specified SNR and steady-state phase error. It also presents a simple approximate formula that is asymptotically tight at higher loop SNR.

Tausworthe, R. C.↗

Delta modulation

The results are presented of a study dealing with the problems of source encoding using delta modulation. An algorithm is described which was employed in the design of an adaptive delta modulation. The algorithm is extremely inexpensive to implement and features extremely desirable characteristics such as a 40 db dynamic range and a 90 percent non-redundant word intelligibility at a bit rate of 9.6 kb/s. A new concept is presented in delta modulation design, the nth-order delta modulator. The nth order delta modulator increases the maximum SNR of a delta modulator but not its dynamic range. It is shown that a 2nd order delta modulator, which employs two delta modulators in cascade, results in a 15 db output SNR improvement over a linear delta modulator operating at 27 db. Two additional areas of research are discussed. The first area involves decomposition of voice and the second is concerned with the source encoding of video signals.

Schilling, D. L.↗

Design of communication systems using short-constraint-length convolutional codes.

A method is presented for calculating the effect of carrier-phase reference error in a receiver phase-locked loop on bit error rates in convolutionally coded data, for the practical design of systems using short-constraint-length convolutional codes. A set of design curves shows the relation between design bit error rate, uplink carrier tracking loop SNR, downlink total SNR, and downlink modulation index. These curves make possible rapid and simple system optimization.

Merrill, H. M.↗

Preemphasis for an S-band constant bandwidth FM/FM system.

Preemphasis schedules are given for 11 constant-bandwidth FM subcarriers modulating an S-band transmitter at various receiver SNRs (9 dB, 15 dB and 25 dB). The criterion for establishing these preemphasis curves is the achievement, at various receiver IF SNR, of equal receiver output SNR for all channels. The empirically derived results are compared with a simplified, analytically derived schedule and the primary differences are explained. The S-band preemphasis schedule is also found to differ from the lower frequency VHF case.

Wallace, G. R.↗

Optimal space communications techniques

Digital multiplication of two waveforms using delta modulation (DM) is discussed. It is shown that while conventional multiplication of two N bit words requires N2 complexity, multiplication using DM requires complexity which increases linearly with N. Bounds on the signal-to-quantization noise ratio (SNR) resulting from this multiplication are determined and compared with the SNR obtained using standard multiplication techniques. The phase locked loop (PLL) system, consisting of a phase detector, voltage controlled oscillator, and a linear loop filter, is discussed in terms of its design and system advantages. Areas requiring further research are identified.

Schilling, D. L.↗

Quantizing and sampling considerations in digital phased-locked loops

The quantizer problem is first considered. The conditions under which the uniform white sequence model for the quantizer error is valid are established independent of the sampling rate. An equivalent spectral density is defined for the quantizer error resulting in an effective SNR value. This effective SNR may be used to determine quantized performance from infinitely fine quantized results. Attention is given to sampling rate considerations. Sampling rate characteristics of the digital phase-locked loop (DPLL) structure are investigated for the infinitely fine quantized system. The predicted phase error variance equation is examined as a function of the sampling rate. Simulation results are presented and a method is described which enables the minimum required sampling rate to be determined from the predicted phase error variance equations.

Hurst, G. T.↗

Signal-to-noise ratios in coherent soft limiters

Expressions for the output signal-to-noise power ratio of a bandpass soft limiter followed by a coherent detection device are presented and discussed. It is found that a significant improvement in the output signal-to-noise ratio (SNR) at low input SNR's can be achieved by such soft limiters as compared to hard limiters. This indicates that the soft limiter may be of some use in the area of threshold extension. Approximation methods for determining output signal-to-noise spectral densities are also presented.

Lesh, J. R.↗