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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 307 records · Page 17
All sourcesNASA NTRSDOE OSTI
NASA NTRS2001

Molecular motors and their functions in plants

Molecular motors that hydrolyze ATP and use the derived energy to generate force are involved in a variety of diverse cellular functions. Genetic, biochemical, and cellular localization data have implicated motors in a variety of functions such as vesicle and organelle transport, cytoskeleton dynamics, morphogenesis, polarized growth, cell movements, spindle formation, chromosome movement, nuclear fusion, and signal transduction. In non-plant systems three families of molecular motors (kinesins, dyneins, and myosins) have been well characterized. These motors use microtubules (in the case of kinesines and dyneins) or actin filaments (in the case of myosins) as tracks to transport cargo materials intracellularly. During the last decade tremendous progress has been made in understanding the structure and function of various motors in animals. These studies are yielding interesting insights into the functions of molecular motors and the origin of different families of motors. Furthermore, the paradigm that motors bind cargo and move along cytoskeletal tracks does not explain the functions of some of the motors. Relatively little is known about the molecular motors and their roles in plants. In recent years, by using biochemical, cell biological, molecular, and genetic approaches a few molecular motors have been isolated and characterized from plants. These studies indicate that some of the motors in plants have novel features and regulatory mechanisms. The role of molecular motors in plant cell division, cell expansion, cytoplasmic streaming, cell-to-cell communication, membrane trafficking, and morphogenesis is beginning to be understood. Analyses of the Arabidopsis genome sequence database (51% of genome) with conserved motor domains of kinesin and myosin families indicates the presence of a large number (about 40) of molecular motors and the functions of many of these motors remain to be discovered. It is likely that many more motors with novel regulatory mechanisms that perform plant-specific functions are yet to be discovered. Although the identification of motors in plants, especially in Arabidopsis, is progressing at a rapid pace because of the ongoing plant genome sequencing projects, only a few plant motors have been characterized in any detail. Elucidation of function and regulation of this multitude of motors in a given species is going to be a challenging and exciting area of research in plant cell biology. Structural features of some plant motors suggest calcium, through calmodulin, is likely to play a key role in regulating the function of both microtubule- and actin-based motors in plants.

Non-NASA Center↗
NASA NTRS1972

The pseudonoise test set: Communication system's performance evaluation based upon RMS error testing

A pseudonoise (PN) test set was built to provide a relatively easy means of accurately determining the end-to-end rms error introduced by a communication system when subjected to wideband data. It utilizes a filtered pseudorandom sequence generator as a wideband data source, providing a convenient means for digitally delaying the input reference signal for comparison with the distorted test communication system output. In addition to providing a means to measure the end-to-end rms error and the average delay of a communication system, the PN test set also provides a means to determine the tested system's impulse response and correlation function. The theory of PN testing is discussed in detail along with the most difficult aspects of implementation, the building of matched filter pairs. Both analytical and empirical results are reported which support the contentions that this is an accurate and practical way to acquire figures of merit for complete communication systems.

Wallace, G. R.↗
NASA NTRS1974

Evaluation of S190A radiometric exposure test data

The S190A preflight radiometric exposure test data generated as part of preflight and system test of KM-002 Sequence 29 on flight camera S/N 002 was analyzed. The analysis was to determine camera system transmission using available data which included: (1) films exposed to a calibrated light source subject; (2) filter transmission data; (3) calibrated light source data; (4) density vs. log10 exposure curves for the films; and (5) spectral sensitometric data for the films. The procedure used is outlined, and includes the data and a transmission matrix as a function of field position for nine measured points on each station-film-filter-aperture-shutter speed combination.

Lockwood, H. E.↗
NASA NTRS1987

Oscillator strengths for S I, S II, and S III

A series of calculations for atomic data of various sulfur and oxygen ions is examined. Recent observations of the Io plasma torus obtained with the Voyager UV Spectrometer, the IUE satellite short wavelength spectrograph, and the rocket-borne faint object telescope are discussed. The calculation of oscillator strengths for S II, the P I sequence, S I, and S III in terms of configuration interaction effects is described. The derivation of orbital wave functions is considered. The use of the close coupling method to estimate collision strengths is studied. The accuracy of these calculations depends on: (1) the number of states used in the close coupling expansion; (2) resonance contributions to the thermally averaged collision strength; and (3) the quality of the target state wave functions. Tables of the derived oscillator strengths are presented.

Ho, Y. K.↗
NASA NTRS1981

Application of maximum-entropy spectral estimation to deconvolution of XPS data

A comparison is made between maximum-entropy spectral estimation and traditional methods of deconvolution used in electron spectroscopy. The maximum-entropy method is found to have higher resolution-enhancement capabilities and, if the broadening function is known, can be used with no adjustable parameters with a high degree of reliability. The method and its use in practice are briefly described, and a criterion is given for choosing the optimal order for the prediction filter based on the prediction-error power sequence. The method is demonstrated on a test case and applied to X-ray photoelectron spectra.

Vasquez, R. P.↗
NASA NTRS1998

Expression of extracellular calcium (Ca2+o)-sensing receptor in human peripheral blood monocytes

The calcium-sensing receptor (CaR) is a G protein-coupled receptor playing key roles in extracellular calcium ion (Ca2+o) homeostasis in parathyroid gland and kidney. Macrophage-like mononuclear cells appear at sites of osteoclastic bone resorption during bone turnover and may play a role in the "reversal" phase of skeletal remodeling that follows osteoclastic resorption and precedes osteoblastic bone formation. Bone resorption produces substantial local increases in Ca2+o that could provide a signal for such mononuclear cells present locally within the bone marrow microenvironment. Indeed, previous studies by other investigators have shown that raising Ca2+o either in vivo or in vitro stimulated the release of interleukin-6 (IL-6) from human peripheral blood monocytes, suggesting that these cells express a Ca2+o-sensing mechanism. In these earlier studies, however, the use of reverse transcription-polymerase chain reaction (RT-PCR) failed to detect transcripts for the CaR previously cloned from parathyroid and kidney in peripheral blood monocytes. Since we recently found that non-specific esterase-positive, putative monocytes isolated from murine bone marrow express the CaR, we reevaluated the expression of this receptor in human peripheral blood monocytes. Immunocytochemistry, flow cytometry, and Western blot analysis, performed using a polyclonal antiserum specific for the CaR, detected CaR protein in human monocytes. In addition, the use of RT-PCR with CaR-specific primers, followed by nucleotide sequencing of the amplified products, identified CaR transcripts in the cells. Therefore, taken together, our data show that human peripheral blood monocytes possess both CaR protein and mRNA very similar if not identical to those expressed in parathyroid and kidney that could mediate the previously described, direct effects of Ca2+o on these cells. Furthermore, since mononuclear cells isolated from bone marrow also express the CaR, the latter might play some role in the "reversal" phase of bone remodeling, sensing local changes in Ca2+o resulting from osteoclastic bone resorption and secreting osteotropic cytokines or performing other Ca2+o-regulated functions that contribute to the control of bone turnover.

NASA Discipline Musculoskeletal↗
NASA NTRS1996

DKRZ workload analysis

The UniTree product as it is released emits a rather large amount of logging information into various log files, but this data typically is meant for operator information in difficult operational situations or directly for debugging purposes. It is strongly advised that the existing logging functionality in the standard release is advanced by adding messages with relevant parameters for every major event in the course of executing file or device oriented requests as described. This upgrade would be relatively easy in terms of implementation effort. The benefit is a complete sequence of transaction descriptions generated by external user requests or by internal administration commands. This collection of transaction records can be used for intensive statistics and performance evaluations. It is furthermore a perfect source to drive realistic system simulations to study the effects of possible hardware or software changes.

Fichter, Hartmut↗
NASA NTRS1999

Mechanosensitive channels in bacteria as membrane tension reporters

The purpose of this short review is to discuss recent data on the molecular structure and mechanism of gating of MscL, a mechanosensitive channel of large conductance from Escherichia coli. MscL is the first isolated molecule shown to convert mechanical stress of the membrane into a simple response, the opening of a large aqueous pore. The functional complex appears to be a stable homo-pentamer of 15-kDa subunits, the gating transitions in which are driven by stretch forces conveyed through the lipid bilayer. We have measured the open probability of MscL and the kinetics of transitions as a function of membrane tension. The parameters extracted from the single-channel current recordings and dose-response curves such as the energy difference between the closed, open, and intermediate conducting states, and the transition-related changes in protein dimensions suggest a large conformational rearrangement of the channel complex. The estimations show that in native conditions MscL openings could be driven primarily by forces of osmotic nature. The thermodynamic and spatial parameters reasonably correlate with the available data on the structure of a single MscL subunit and multimeric organization of the complex. Combined with the functional analysis of mutations, these data give grounds to hypotheses on the nature of the channel mechanosensitivity.

Review↗
NASA NTRS2002

Predicting functional divergence in protein evolution by site-specific rate shifts

Most modern tools that analyze protein evolution allow individual sites to mutate at constant rates over the history of the protein family. However, Walter Fitch observed in the 1970s that, if a protein changes its function, the mutability of individual sites might also change. This observation is captured in the "non-homogeneous gamma model", which extracts functional information from gene families by examining the different rates at which individual sites evolve. This model has recently been coupled with structural and molecular biology to identify sites that are likely to be involved in changing function within the gene family. Applying this to multiple gene families highlights the widespread divergence of functional behavior among proteins to generate paralogs and orthologs.

Review↗
NASA NTRS1975

Design automation techniques for custom LSI arrays

The standard cell design automation technique is described as an approach for generating random logic PMOS, CMOS or CMOS/SOS custom large scale integration arrays with low initial nonrecurring costs and quick turnaround time or design cycle. The system is composed of predesigned circuit functions or cells and computer programs capable of automatic placement and interconnection of the cells in accordance with an input data net list. The program generates a set of instructions to drive an automatic precision artwork generator. A series of support design automation and simulation programs are described, including programs for verifying correctness of the logic on the arrays, performing dc and dynamic analysis of MOS devices, and generating test sequences.

Feller, A.↗
NASA NTRS1993

Star counts from the Hubble Space Telescope Snapshot Survey. I - Galactic models

We report a photometric study of stars from 450 fields at high Galactic latitudes that were observed in the Hubble Space Telescope Snapshot Survey to an average limiting apparent magnitude of V = 21.4. There are 166 fields that contain quasars selected in radio, X-ray, and color-excess surveys. This sample of 273 stars is free of selection bias with respect to the density of stars. To within the Poisson errors, the total counts and magnitude distribution of this unbiased sample are in agreement with the Bahcall-Soneira model (Bahcall, 1986). The angular distribution of the faint stars favors, at the 2 sigma level, a somewhat steeper disk luminosity function and a smaller spheroid main-sequence normalization than given by the model. The sample does not have enough statistical power to distinguish between the two-component Bahcall-Soneira model and the three-component model first proposed by Gilmore and Reid (1983), which contains a thick disk. The statistical power of the survey would increase about 15-fold if colors were obtained for the stars: the data probe the main sequences of the disk, thick disk, and spheroid. Models with and without a thick disk could then be distinguished at the 6 sigma level. The HST Snapshot Survey includes an additional 284 fields, 279 of which are centered on quasars that were selected by objective-prism surveys. These 279 fields are expected to show and do exhibit bias against bright stars, making them unsuitable for testing Galactic models.

Gould, A.↗
NASA NTRS1993

[Genotoxic modification of nucleic acid bases and biological consequences of it. Review and prospects of experimental and computational investigations]

The review is presented of experimental and computational data on the influence of genotoxic modification of bases (deamination, alkylation, oxidation) on the structure and biological functioning of nucleic acids. Pathways are discussed for the influence of modification on coding properties of bases, on possible errors of nucleic acid biosynthesis, and on configurations of nucleotide mispairs. The atomic structure of nucleic acid fragments with modified bases and the role of base damages in mutagenesis and carcinogenesis are considered.

Non-NASA Center↗
NASA NTRS1976

Sequence characterization of 5S ribosomal RNA from eight gram positive procaryotes

Complete nucleotide sequences are presented for 5S rRNA from Bacillus subtilis, B. firmus, B. pasteurii, B. brevis, Lactobacillus brevis, and Streptococcus faecalis, and 5S rRNA oligonucleotide catalogs and partial sequence data are given for B. cereus and Sporosarcina ureae. These data demonstrate a striking consistency of 5S rRNA primary and secondary structure within a given bacterial grouping. An exception is B. brevis, in which the 5S rRNA sequence varies significantly from that of other bacilli in the tuned helix and the procaryotic loop. The localization of these variations suggests that B. brevis occupies an ecological niche that selects such changes. It is noted that this organism produces antibiotics which affect ribosome function.

Woese, C. R.↗
DOE OSTI2026

Data for Development, Optimization, and Application of an Episomal Plasmid System for Rhodotorula toruloides

Rhodotorula toruloides is an emerging oleaginous yeast with strong potential as a microbial cell factory for the production of acetyl-CoA-derived bioproducts. However, engineering of this organism has been limited by the absence of a functional episomal plasmid system, a foundational genetic tool for rapid gene expression, pathway testing, and CRISPR-based genome engineering. Here, we report the first episomal plasmid system for R. toruloides . Through systematic screening of candidate autonomously replicating sequences (ARSs) from diverse sources, we identified multiple functional ARS elements and selected C63F4, a fragment derived from Contig 63 of R. toruloides CBS14, because of its stable performance. The resulting pC63F4 plasmid was maintained episomally, supported GFP reporter expression, exhibited a copy number of 2.39 ± 0.13, and showed good stability during long term cultivation. To overcome poor transformation efficiency, we developed a Cre-loxP-mediated in vivo re-circularization strategy that enabled reliable delivery of the episomal plasmid. Using this improved system, we demonstrated functional episomal expression of metabolic engineering genes and multi-gene pathways for the production of triacetic acid lactone, fatty alcohols, and limonene. Finally, we leveraged this platform to establish a redesigned CRISPR system that enables seamless genome editing in R. toruloides for the first time, while also simplifying marker recycling. Together, this work establishes a long-needed episomal plasmid platform and associated CRISPR toolkit that will accelerate metabolic engineering, synthetic biology, and fundamental studies in R. toruloides .

Gene Editing↗
NASA NTRS1978

Interplanetary approach optical navigation with applications

The use of optical data from onboard television cameras for the navigation of interplanetary spacecraft during the planet approach phase is investigated. Three optical data types were studied: the planet limb with auxiliary celestial references, the satellite-star, and the planet-star two-camera methods. Analysis and modelling issues related to the nature and information content of the optical methods were examined. Dynamic and measurement system modelling, data sequence design, measurement extraction, model estimation and orbit determination, as relating optical navigation, are discussed, and the various error sources were analyzed. The methodology developed was applied to the Mariner 9 and the Viking Mars missions. Navigation accuracies were evaluated at the control and knowledge points, with particular emphasis devoted to the combined use of radio and optical data. A parametric probability analysis technique was developed to evaluate navigation performance as a function of system reliabilities.

Jerath, N.↗
NASA NTRS2000

A strong loss-of-function mutation in RAN1 results in constitutive activation of the ethylene response pathway as well as a rosette-lethal phenotype

A recessive mutation was identified that constitutively activated the ethylene response pathway in Arabidopsis and resulted in a rosette-lethal phenotype. Positional cloning of the gene corresponding to this mutation revealed that it was allelic to responsive to antagonist1 (ran1), a mutation that causes seedlings to respond in a positive manner to what is normally a competitive inhibitor of ethylene binding. In contrast to the previously identified ran1-1 and ran1-2 alleles that are morphologically indistinguishable from wild-type plants, this ran1-3 allele results in a rosette-lethal phenotype. The predicted protein encoded by the RAN1 gene is similar to the Wilson and Menkes disease proteins and yeast Ccc2 protein, which are integral membrane cation-transporting P-type ATPases involved in copper trafficking. Genetic epistasis analysis indicated that RAN1 acts upstream of mutations in the ethylene receptor gene family. However, the rosette-lethal phenotype of ran1-3 was not suppressed by ethylene-insensitive mutants, suggesting that this mutation also affects a non-ethylene-dependent pathway regulating cell expansion. The phenotype of ran1-3 mutants is similar to loss-of-function ethylene receptor mutants, suggesting that RAN1 may be required to form functional ethylene receptors. Furthermore, these results suggest that copper is required not only for ethylene binding but also for the signaling function of the ethylene receptors.

NASA Discipline Plant Biology↗
DOE OSTI2026

RCSB protein data Bank: Next‐generation advanced search for exploration of experimental structures and computed structure models

Abstract The Protein Data Bank (PDB), established in 1971, is the primary global, open‐access archive for experimentally determined 3D macromolecular structures (proteins, RNA, DNA). The research‐focused RCSB.org web‐portal provides access to these data alongside more than one million machine‐learning‐predicted structure models, greatly expanding the available structural landscape. Rapid growth of both experimental and computational structures has increased the need for powerful yet accessible search tools that serve a broad and diverse scientific community. Herein, we describe a redesigned RCSB Protein Data Bank RCSB.org Advanced Search capability that supports intuitive discovery of 3D structures through a unified interface. This interface integrates annotation‐, sequence‐, and 3D structure‐based searches, embeds an interactive 3D viewer, and incorporates curated biological knowledge, such as catalytic site definitions from Mechanism and Catalytic Site Atlas and ligand‐guided structural motifs, for constructing geometry‐driven queries. A new Chemical Search tool allows definition of chemical queries via an integrated drawing tool or standard identifiers, seamlessly combining them with annotation filters. By allowing query definition directly within spatial and chemical contexts, these search interfaces reduce the need for detailed knowledge of residue numbering, chain identifiers, or external cheminformatics software. This capability enables efficient exploration of structures, chemical diversity, and structure–function relationships across all life domains. The redesigned interfaces can be accessed directly at rcsb.org/search/advanced for Advanced Search and rcsb.org/search/chemical for Chemical Search.

Rose, Yana [Research Collaboratory for Structural ↗
NASA NTRS1973

Mariner 9 extended mission sequence design optimization

This paper discusses the Mariner 9 science sequence design during the extended mission including science objectives, and mission operation and spacecraft constraints. The primary objectives of the extended mission were to complete 100% mapping of the planet, photograph special interest targets, obtain high resolution coverage of prospective Viking landing sites, and to function through the superior conjunction period. The optimization of planet viewing characteristics by moving to different celestial reference stars, and the techniques used to efficiently allocate science links to data taking days are described.

Reichert, R. J.↗