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At least 307 records · Page 17

Hydrogen Biogeochemistry in Anaerobic and Photosynthetic Ecosystems

The simple biochemistry of molecular hydrogen is central to a large number of microbial processes, affecting the interaction of organisms with each other and with the environment. In anoxic sediments, a great majority of microbial redox processes involve hydrogen as a reactant, product or potential by-product. Accordingly, the energetics (thermodynamics) of each of these processes is affected by variations in local H2 concentrations. It has long been established that this effect is important in governing microbe-microbe interactions and there are multiple demonstrations that "interspecies hydrogen transfer" can alter the products of, inhibit/stimulate, or even reverse microbial metabolic reactions. In anoxic sediments, H2 concentrations themselves are thought to be controlled by the thermodynamics of the predominant H2-consuming microbial process. In sediments from Cape Lookout Bight, this relationship quantitatively describes the co-variation of H2 concentrations with temperature (for methanogens and sulfate reducers) and with sulfate concentration (for sulfate reducers). The quantitative aspect is import= for two reasons: 1) it permits the modeling of H2-sensitive biogeochemistry, such as anaerobic methane oxidation or pathways of organic matter remineralization, as a function of environmental controls; 2) for such a relationship to be observed requires that intracellular biochemistry and bioenergetics are being directly expressed in a component of the extracellular medium. H2 could therefore be utilized a non-invasive probe of cellular energetic function in intact microbial ecosystems. Based on the latter principle we have measured down-core profiles of H2 and other relevant physico-chemical parameters in order to calculate the metabolic energy yields (DG) that support microbial metabolism in Cape Lookout Bight sediments. Methanogens in this system apparently function with energy yields significantly smaller than the minimum requirements suggested by pure culture studies. Our recent work has extended the study of hydrogen to cyanobacterial mat communities. The large amounts of reducing power generated during photosynthetic activity carry the potential to contribute a swamping term to the H2 economy of the anaerobic microbial populations within the mat - and thereby to alter the population structure and biogeochemical function of the mat as a whole. In hypersaline microbial mats, we observe a distinct diel cycle in H2 production and a substantial corresponding flux. On an early Earth dominated by microbial mats, this transmission of photosynthetic reducing power may have carried important implications for both biospheric and atmospheric evolution.

Hoehler, Tori M.↗

Characteristics of human dendritic cells generated in a microgravity analog culture system

Generation of an effective immune response requires that antigens be processed and presented to T lymphocytes by antigen-presenting cells, the most efficient of which are dendritic cells (DC). Because of their influence on both the innate and the acquired arms of immunity, a defect in DC would be expected to result in a broad impairment of immune function, not unlike that observed in astronauts during or after space flight. In the study reported here, we investigated whether DC generation and function are altered in a culture environment that models microgravity, i.e., the rotary-cell culture system (RCCS). We observed that RCCS supported the generation of DC identified by morphology, phenotype (HLA-DR+ and lacking lineage-associated markers), and function (high allostimulatory activity). However, the yield of DC from RCCS was significantly lower than that from static cultures. RCCS-generated DC were less able to phagocytose Aspergillus fumigatus conidia and expressed a lower density of surface HLA-DR. The proportion of DC expressing CD80 was also significantly reduced in RCCS compared to static cultures. When exposed to fungal antigens, RCCS-generated DC produced lower levels of interleukin-12 and failed to upregulate some costimulatory/adhesion molecules involved in antigen presentation. These data suggest that DC generation, and some functions needed to mount an effective immune response to pathogens, may be disturbed in the microgravity environment of space.

Non-NASA Center↗

Satellite observations of snow and ice with an imaging passive microwave spectrometer

The scanning microwave spectrometer (SCAMS) on the Nimbus-6 satellite continuously maps the terrestrial surface with a resolution of about 150 km at 22.235 and 31.400 GHz. SCAMS observes at six angles besides nadir, yielding brightness temperatures which are a function of the distribution and character of various types of snow and ice, including microstructure and subsurface profiles in refractive index, loss (moisture or salinity), and temperature. Spectral signatures exhibiting interesting topographical structure have been observed. To aid in the interpretation of these data, a model was developed to describe the propagation of microwave intensity in a scattering medium characterized by three-dimensional random fluctuations of refractive index in addition to nonrandom variations in permittivity, temperature, and loss. The model combines Maxwell's equations in the Born approximation with radiative-transfer theory; this approach yields the variation of intensity with polarization, direction, and position.

Fisher, A. D.↗

Classifying multispectral data by neural networks

Several energy functions for synthesizing neural networks are tested on 2-D synthetic data and on Landsat-4 Thematic Mapper data. These new energy functions, designed specifically for minimizing misclassification error, in some cases yield significant improvements in classification accuracy over the standard least mean squares energy function. In addition to operating on networks with one output unit per class, a new energy function is tested for binary encoded outputs, which result in smaller network sizes. The Thematic Mapper data (four bands were used) is classified on a single pixel basis, to provide a starting benchmark against which further improvements will be measured. Improvements are underway to make use of both subpixel and superpixel (i.e. contextual or neighborhood) information in tile processing. For single pixel classification, the best neural network result is 78.7 percent, compared with 71.7 percent for a classical nearest neighbor classifier. The 78.7 percent result also improves on several earlier neural network results on this data.

Telfer, Brian A.↗

An analysis of scatter decomposition

A formal analysis of a powerful mapping technique known as scatter decomposition is presented. Scatter decomposition divides an irregular computational domain into a large number of equal sized pieces, and distributes them modularly among processors. A probabilistic model of workload in one dimension is used to formally explain why, and when scatter decomposition works. The first result is that if correlation in workload is a convex function of distance, then scattering a more finely decomposed domain yields a lower average processor workload variance. The second result shows that if the workload process is stationary Gaussian and the correlation function decreases linearly in distance until becoming zero and then remains zero, scattering a more finely decomposed domain yields a lower expected maximum processor workload. Finally it is shown that if the correlation function decreases linearly across the entire domain, then among all mappings that assign an equal number of domain pieces to each processor, scatter decomposition minimizes the average processor workload variance. The dependence of these results on the assumption of decreasing correlation is illustrated with situations where a coarser granularity actually achieves better load balance.

Nicol, David M.↗

A significant post-launch calibration experiment for the SEASAT-A SAR

A technique for periodically monitoring the impulse response function of the SEASAT-A synthetic aperture radar is outlined that yields most of the significant contributors to the overall instrument transfer function. The essential measurement involves exciting the total SAR system at L-band with a strong point source scatterer, receiving via the normal S-band analog data link, and finally sampling and processing only a small subset of data in the immediate vicinity of the strong point source.

Beal, R. C.↗

Green's formula and variational principles for cosmic-ray transport with application to rotating and shearing flows

Green's theorem and Green's formula for the diffusive cosmic-ray transport equation in relativistic flows are derived. Green's formula gives the solution of the transport equation in terms of the Green's function of the adjoint transport equation, and in terms of distributed sources throughout the region R of interest, plus terms involving the particle intensity and streaming on the boundary. The adjoint transport equation describes the time-reversed particle transport. An Euler-Lagrange variational principle is then obtained for both the mean scattering frame distribution function f, and its adjoint f(dagger). Variations of the variational functional with respect to f(dagger) yield the transport equation, whereas variations of f yield the adjoint transport equation. The variational principle, when combined with Noether's theorem, yields the conservation law associated with Green's theorem. An investigation of the transport equation for steady, azimuthal, rotating flows suggests the introduction of a new independent variable H to replace the comoving frame momentum variable p'. For the case of rigid rotating flows, H is conserved and is shown to be analogous to the Hamiltonian for a bead on a rigidly rotating wire. The variable H corresponds to a balance between the centrifugal force and the particle inertia in the rotating frame. The physical interpretation of H includes a discussion of nonrelativistic and special relativistic rotating flows as well as the cases of aziuthal, differentially rotating flows about Schwarzs-child and Kerr black holes. Green's formula is then applied to the problem of the acceleration of ultra-high-energy cosmic rays by galactic rotation. The model for galactic rotation assumes an angular velocity law Omega = Omega(sub 0)(omega(sub 0)/omega), where omega denotes radial distance from the axis of rotation. Green's functions for the galactic rotation problem are used to investigate the spectrum of accelerated particles arising from monoenergetic and truncated power-law sources. We conclude that it is possible to accelerate particles beyond the knee by galactic rotation, but not in sufficient number to adequately explain the observed spectrum.

Webb, G. M.↗

Transmission function for infrared radiative transfer in an inhomogeneous atmosphere

Calculations of the transmission of infrared radiation through the atmosphere must take into account inhomogeneities in the path due to variations of temperature, pressure and absorbing matter. For ozone absorption the method due to Curtis and Godson does not seem to yield results that are sufficiently precise. In this paper an improved approximation is developed that yields an analytic expression for the transmission function and this is compared with the exact results obtained from the Goody random model.

Kuriyan, J. G.↗

LIF measurements of scalar mixing in turbulent shear layers

The structure of shear layer flows at high Reynolds numbers remains a very interesting problem. Straight mixing layers have been studied and yielded information on the probability density function (pdf) of a passive scalar across the layer. Konrad and Koochesfahani & Dimotakis measured the pdf of the mixture fraction for mixing layers of moderate Reynolds numbers, each about 25,000 (Re based on velocity difference and visual thickness). Their measurements showed a 'non-marching' pdf (central hump which is invariant from edge to edge across the layer), a result which is linked to the visualizations of the spanwise Kelvin-Helmholtz (K-H) instability mode, which is the primary instability for plane shear layer flows. A secondary instability mode, the Taylor-Gortler (T-G) instability, which is associated with streamwise vortical structures, has also been observed in shear layers. Image reconstruction by Jimenez et al. and volume renderings by Karasso & Mungal at low Re numbers have demonstrated that the K-H and the T-G instability modes occur simultaneously in a non-mutually destructive way, evidence that supports the quasi two-dimensional aspect of these flows and the non-marching character of the pdf at low Reynolds numbers. At higher Re numbers though, the interaction of these two instability modes is still unclear and may affect the mixing process. In this study, we perform measurements of the concentration pdf of plane mixing layers for different operating conditions. At a speed ratio of r = U(sub 1)/U(sub 2) = 4:1, we examine three Reynolds number cases: Re = 14,000, Re = 31,000, and Re = 62,000. Some other Re number cases' results, not presented in detail, are invoked to explain the behavior of the pdf of the concentration field. A case of r = 2.6:1 at Re = 20,000 is also considered. The planar laser-induced fluorescence technique is used to yield quantitative measurements. The different Re are obtained by changing the velocity magnitudes of the two streams. The question of resolution of these measurements is addressed. In order to investigate the effects of the initial conditions on the development and the structure of the mixing layer, the boundary layer on the high-speed side of the splitter plate is tripped. The average concentration and the average mixed fluid concentration are also calculated to further understand the changes in the shear layer for the different cases examined.

Karasso, Paris S.↗

Recovering Aerodynamic Side Loads on Rocket Nozzles using Quasi-Static Strain-Gage Measurements

During over-expanded operation of rocket nozzles, which is defined to be when the exit pressure is greater than internal pressure over some part of the nozzle, the nozzle will experience a transverse forcing function due to the pressure differential across the nozzle wall. Over-expansion occurs during the nozzle start-up and shutdown transient, even in high-altitude engines, because most test facilities cannot completely reproduce the near-vacuum pressures at those altitudes. During this transient, the pressure differential moves axially down the nozzle as it becomes pressurized, but this differential is never perfectly symmetric circumferentially. The character of the forcing function is highly complex and defined by a series of restricted and free shock separations. The subject of this paper is the determination of the magnitude of this loading during sub-scale testing via measurement of the structural dynamic response of the nozzle and its support structure. An initial attempt at back-calculating this load using the inverse of the transfer function was performed, but this attempt was shown to be highly susceptible to numerical error. The final method chosen was to use statically calibrated strain data and to filter out the system fundamental frequency such that the measured response yields close to the correct dynamic loading function. This method was shown to capture 93% of the pressure spectral energy using controlled load shaker testing. This method is one of the only practical ways for the inverse determination of the forcing function for non-stationary excitations, and, to the authors' knowledge, has not been described in the literature to date.

Brown, Andrew↗

Methods and approaches to dosimetry

The development of a dosimetric system capable of determining energy depositions in tissue regions that are smaller than a few 100 nanometers is projected. These objectives are met by evaluation of the data produced by a macro-subsystem and a micro-subsystem. Both systems are in essence multiple ionization chambers that are normally operated in a gated pulse mode. The macro-system yields absorbed radiation dose as a function of location in a phantom of the human trunk when it operates in the dose mode; it registers only those sections as a signal in which the primary particle or any of its secondaries have passed, in the pulse mode. The function of the micro-system is to provide detailed information of the track structure by determining lateral energy spread due to delta ray formation or other secondary particle production.

Rossi, H. H.↗

An evaluation of space acquired data as a tool for management to wildlife habitat in Alaska

The Bureau of Sport Fisheries and Wildlife ERTS experiment in Alaska attempts to yield information useful for three primary functions in the State. They are: (1) to test the feasibility of using ERTS data, in conjunction with aircraft acquired multispectral photography, to develop effective stratified sampling techniques, (2) to provide near real time assessment and evaluation of the quantity, quality, and distribution of waterfowl breeding habitat through frequent ERTS measurements of hydrologic, phenological and vegetational parameters, and (3) to provide basic mapping of vegetation and terrain in certain remote areas of the State for which little or no biological data now exist.

Vantries, B. J.↗

Ultrahigh vacuum measuring ionization gauge

The ionization gage described consists of separate ionization and collector regions connected at an exit area with a modulator electrode. In addition to the standard modulation function, the modulator in this location yields a suprising increase in collector current, apparently due to improved focussing and extraction of ions from the ionization region.

Brock, F. J.↗

Hydrogen atom in intense magnetic field.

The structure of a hydrogen atom situated in an intense magnetic field is investigaged. Three approaches are employed. An elementary Bohr picture establishes a crucial magnetic field strength, H sub a approximately equal to 5 x 10 to the 9th G. Fields in excess of H sub a are intense in that they are able to modify the characteristic atomic scales of length and binding energy. A second approach solves the Schrodinger equation by a combination of variational methods and perturbation theory. It yields analytic expressions for the wave functions and energy eigenvalues. A third approach determines the energy eigenvalues by reducing the Schrodinger equation to a one-dimensional wave equation, which is then solved numerically. Energy eigenvalues are tabulated for field strengths of 2 x 10 to the 10th G and 2 x 10 to the 12th G. It is found that at 2 x 10 to the 12th G the lowest energy eigenvalue is changed from -13.6 to about -180 eV in agreement with previous variational computations.

Canuto, V.↗

Effect of axial load on mode shapes and frequencies of beams

An investigation of the effect of axial load on the natural frequencies and mode shapes of uniform beams and of a cantilevered beam with a concentrated mass at the tip is presented. Characteristic equations which yield the frequencies and mode shape functions for the various cases are given. The solutions to these equations are presented by a series of graphs so that frequency as a function of axial load can readily be determined. The effect of axial load on the mode shapes are also depicted by another series of graphs.

Shaker, F. J.↗