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Mineral exploration and fracture trends in Utah and Nevada, by ERTS-1 imagery

The author has identified the following significant results. Major structural trends have been compiled on five separate maps, at a scale of 1:1,000,000, of Utah and Nevada from ERTS-1 imagery. An arbitrary length of ten kilometers has been chosen as a minimum length of the trends. The selection is based upon: (1) obvious displacement of structures; (2) continuity or persistence of trends across structures; (3) line-up of outcrop patterns, drainage, erosional features or vegetation; and (4) near-linear trends. Several recognizable trend directions have noted, viz., N 10-15 deg W, N 35 deg W, N 80 deg W, N 30 deg E, and E-W. More than 1500 structural trends have been identified, some of which are mineralized and extend into pediment or shallow alluvial cover. Those fracture trends that exhibit mineralization in exposed bedrock will be assayed for mercury content with a soil-gas analyzer and similar collection will be done over alluvial posture blankets by the same technique with the hope of discovering blind mineralized zones that are not exposed on the surface.

Jensen, M. L.↗

Western Sector In-Situ Chemical Oxidation Project: Additional Injection Pilot Testing Supplemental Results (U)

Trichloroethylene (TCE) and tetrachloroethylene (PCE) are persistent at elevated concentrations in the Western Sector of the M-Area Hazardous Waste Management Facility (HWMF) groundwater plume. Migration of these chlorinated ethenes as Dense Non-Aqueous Phase Liquids (DNAPLs) occurred from the M-Area Settling Basin along geologic strata resulting in a high concentration groundwater plume in Western Sector. Pump-and-treat has been active for over 30 years at the M-Area HWMF to hydraulically contain the primary sources of the plume in addition to other remediation strategies throughout the contaminated zone, like soil vapor extraction, dynamic underground stripping, groundwater recirculation wells, and bioremediation. In-situ chemical oxidation (ISCO) has been identified as a viable treatment method for removal of these volatile organic compounds (VOCs) due to the complex lithology and high concentrations in this part of the aquifer. In addition to ISCO, a new recovery well (i.e., RWM018) was also installed in Western Sector to contain the high concentration VOC plume. The ISCO projects have utilized the gradient induced by RWM018 to help plan the injection and monitoring locations. The combination of ISCO and the additional pump and treat in Western Sector with the installation of RWM018 will help to control the plume and maximize remediation efforts.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

SMAP RFI Change Detection

The Soil Moisture Active Passive (SMAP) mission, launched on January 31, 2015, has completed its primary 3-year mission and is currently in its extended mission. Although operation occurs within the protected frequency allocation of 1400-1427 MHz, the SMAP radiometer is impacted by radio frequency interference (RFI). The radiometer was designed to provide detection and filtering of RFI in order to meet error budget requirements. A time series algorithm was developed to monitor, detect and report the changing environment with the objective of detecting new RFI sources as well as existing persistent sources. The detection of sources are used in RFI reporting to NASA spectrum management with the hope that interfering sources will be turned off by the necessary administrations.

Priscilla N Mohammed↗

Geochemistry and metagenomics analyses of bacterial community structure in selected waste dumpsites in Lagos Metropolis, Nigeria

Dumpsites are reservoirs of persistent organic pollutants (POPs) and heavy metals (HMs), constituting environmental hazards to humanity. Autochthonous microorganisms in dumpsites exhibit various degrees of responses to contaminants. Unfortunately, there is a dearth of information on the types and concentration of pollutants and the array of microorganisms in these dumpsites which may play important roles in the metabolism of such pollutants or other community processes. Therefore, determining the microbial community structure in such contaminated sites across a municipality is essential for profiling the taxa that would serve as consensus degraders of the pollutants. In this study, soil samples from three dumpsites (Cele, CS; Solous, SS; and Computer Village, CVS) were characterized for geochemical properties using GC-MS, MP-AES, and other analytical protocols, while the dynamics of bacterial communities were evaluated based on their 16S rRNA gene barcodes. A significant difference in the bacterial communities was observed among the dumpsites in relation to the extent of pollution caused by POPs and HMs. CVS, with the highest HM contamination, was rich in Actinobacteria (41.7%) and Acidobacteria (10.2%), in contrast to CS and SS. Proteobacteria (34.1%) and Firmicutes (20%) were the dominant phyla in CS (highest POP contamination), while Bacteroidetes (45.5%) and Proteobacteria (39.9%) were dominant in SS soil.Bacilluswas the dominant genus in the most polluted dumpsite. Canonical correspondence analysis revealed that polycyclic aromatic hydrocarbons (PAHs) and HMs shaped the structure of the bacterial operational taxonomic units (OTUs) in the most polluted dumpsite. Out of a total of 706 OTUs, 628 OTUs exhibited a significant correlation (>50%) with benzo(b)fluoranthene, azobenzene, dibenzofurans, pyrene, dibenzo(a,l)pyrene, Cu, and Zn. In particular, Proteobacteria (Achromobactersp. andSerratiasp.), Bacteroidetes (Zhouiasp.), and Firmicutes (Bacillussp.) were suggested to be pivotal to the ecophysiology of dumpsite soils contaminated with POPs and HMs. The results generally underscored the importance of metagenomic and physicochemical analyses of polluted systems in enabling correlations for useful prediction of drivers of such ecosystems. This will further improve our understanding of the metabolic potential and adaptation of organisms in such systems.

Environmental Sciences & Ecology↗

Searching for Extraterrestrial Amino Acids in a Contaminated Meteorite: Amino Acid Analyses of the Canakkale L6 Chondrite

Amino acids can serve as important markers of cosmochemistry, as their abundances and isomeric and isotopic compositions have been found to vary predictably with changes in parent body chemistry and alteration processes. Amino acids are also of astrobiological interest because they are essential for life on Earth. Analyses of a range of meteorites, including all groups of carbonaceous chondrites, along with H, R, and LL chondrites, ureilites, and a martian shergottite, have revealed that amino acids of plausible extraterrestrial origin can be formed in and persist after a wide range of parent body conditions. However, amino acid analyses of L6 chondrites to date have not provided evidence for indigenous amino acids. In the present study, we performed amino acid analysis on larger samples of a different L6 chondite, Canakkale, to determine whether or not trace levels of indigenous amino acids could be found. The Canakkale meteor was an observed fall in late July, 1964, near Canakkale, Turkey. The meteorite samples (1.36 and 1.09 g) analyzed in this study were allocated by C. Y. Ornek, along with a soil sample (1.5 g) collected near the Canakkale recovery site.

Burton, A. S.↗

A Retrospective and Prospective Examination of the 1960s U.S. Northeast Drought

As the most severe drought over the Northeastern United States (NEUS) in the past century, the 1960s drought had pronounced socioeconomic impacts. Although it was followed by a persisting wet period, the conditions leading to the 1960s extreme drought could return in the future, along with its challenges to water management. To project the potential consequences of such a future drought, pseudo-global warming simulations using the Weather Research and Forecasting Model are performed to simulate the dynamical conditions of the historical 1960s drought, but with modified thermodynamic conditions under the shared socioeconomic pathway SSP585 scenario in the early (2021–2027), middle (2041–2047), and late (2091–2097) 21st century. Our analysis focuses on essential hydroclimatic variables including temperature, precipitation, evapotranspiration, soil moisture, snowpack, and surface runoff. In contrast to the historical 1960s drought, similar dynamical conditions will generally produce more precipitation, increased soil moisture and evapotranspiration, and reduced snowpack. However, we also find that although wet months get much wetter, dry months may become drier, meaning that wetting trends are most significant in wet months but are essentially negligible for extremely dry months with negative monthly mean net precipitation. For these months, the trend toward wetting conditions provides little relief from the effects of extreme dry months. These conditions may even aggravate water shortages due to an increasingly rapid transition from wet to dry conditions. Other challenges emerge for residents and stakeholders in this region, including more extreme hot days, record-low snow pack, frozen ground degradation and subsequent decreases in surface runoff.

54 ENVIRONMENTAL SCIENCES↗

The Impact of Devegetated Dune Fields on North American Climate During the Late Medieval Climate Anomaly

During the Medieval Climate Anomaly, North America experienced severe droughts and widespread mobilization of dune fields that persisted for decades. We use an atmosphere general circulation model, forced by a tropical Pacific sea surface temperature reconstruction and changes in the land surface consistent with estimates of dune mobilization (conceptualized as partial devegetation), to investigate whether the devegetation could have exacerbated the medieval droughts. Presence of devegetated dunes in the model significantly increases surface temperatures, but has little impact on precipitation or drought severity, as defined by either the Palmer Drought Severity Index or the ratio of precipitation to potential evapotranspiration. Results are similar to recent studies of the 1930s Dust Bowl drought, suggesting bare soil associated with the dunes, in and of itself, is not sufficient to amplify droughts over North America.

Cook, B. I.↗

Tower-Based Remote Sensing Reveals Mechanisms Behind a Two-phased Spring Transition in a Mixed-Species Boreal Forest

The boreal forest is a major contributor to the global climate system, therefore, reducing uncertainties in how the forest will respond to a changing climate is critical. One source of uncertainty is the timing and drivers of the spring transition. Remote sensing can provide important information on this transition, but persistent foliage greenness, seasonal snow cover, and a high prevalence of mixed forest stands (both deciduous and evergreen species) complicate interpretation of these signals. We collected tower-based remotely sensed data (reflectance-based vegetation indices and Solar-Induced Chlorophyll Fluorescence [SIF]), stem radius measurements, gross primary productivity, and environmental conditions in a boreal mixed forest stand. Evaluation of this data set shows a two-phased spring transition. The first phase is the reactivation of photosynthesis and transpiration in evergreens, marked by an increase in relative SIF, and is triggered by thawed stems, warm air temperatures, and increased available soil moisture. The second phase is a reduction in bulk photoprotective pigments in evergreens, marked by an increase in the Chlorophyll-Carotenoid Index. Deciduous leaf-out occurs during this phase, marked by an increase in all remotely sensed metrics. The second phase is controlled by soil thaw. Our results demonstrate that remote sensing metrics can be used to detect specific physiological changes in boreal tree species during the spring transition. The two-phased transition explains inconsistencies in remote sensing estimates of the timing and drivers of spring recovery. Our results imply that satellite-based observations will improve by using a combination of vegetation indices and SIF, along with species distribution information.

54 ENVIRONMENTAL SCIENCES↗

Sentinel-1 Input Data for PSInSAR Analysis

Files used to perform the Persistent Scatterer InSAR analysis with SARPROZ. The data is sourced from ESAs Sentinel-1 project and covers Brady Hot Springs and Desert Peak geothermal areas. The original titles are included for the Sentinel-1 data. The naming guide is included as a link in this submission. The data contains SAR (Radar) data from the Sentinel 1A satellite between July 2017 and December 2019. The data is necessary to replicate our results, and can be used for further PSInSAR, DInSAR and other modern interferometric analyses to determine line of sight, vertical and, possibly, East-West horizontal displacement. These displacement analysis show the soil deformation in time (including average displacement velocity), which can be used to indicate subsoil phenomena: temperature changes, subsidence, uplift, and inform other analyses like seismicity and porosity.

15 GEOTHERMAL ENERGY↗

germs-lab/PAPER_miscanthus_soil_response

Nutrient inputs influence the sustainability of bioenergy crop production through contemporary (shortly after addition) and legacy effects (persisting over years) on microbial nitrogen (N) and carbon cycling, which contribute to greenhouse gas emissions. However, the relative importance of contemporary and legacy effects and how that could vary by crop functional types is poorly understood. Considering its rhizomatous roots and perennial growth, we hypothesized that Miscanthus × giganteus (M×g) would be more sensitive to legacy N fertilization and the historical context of its environment than an annual crop like maize. To test this hypothesis, we examined the effects of legacy and contemporary N inputs on nitrous oxide (N2O) and carbon dioxide (CO2) emissions, as well as key N cycling genes in soils where M×g and maize were grown. A 150-day soil incubation experiment was conducted using soils from a long-term M×g and maize fertility experiment with three historic N fertilization rates (0, 112, and 336 kg N ha−1 year−1) and a contemporary amendment (60 mg N kg−1) with negative control (0 mg N kg−1). We observed significant increases in cumulative N2O emissions in Mxg soils relative to maize soils, particularly at higher legacy fertilization rates, while contemporary N had no significant effect. Bacterial amoA gene abundance, which plays a significant role in nitrification in nutrient-rich soils, also increased with higher legacy fertilization rates in M×g soils but was unaffected by the contemporary N. In maize soils, legacy and contemporary N did not significantly affect N2O emissions, but cumulative CO2 emissions and amoA gene abundance significantly increased. The abundances of norB genes were not significantly influenced by either legacy fertilization or contemporary N amendments in either soil. Our findings demonstrate the greater importance of fertilization history over contemporary N in mediating soil N2O emissions, particularly for perennial bioenergy crops.

Lee, Jaejin↗

Potential Mars Surveyor 2001 Landing Sites: Low-Elevation Cratered "Highlands" in Central and Eastern Sinus Meridiani and Near Amenthes Fossae

The main scientific goal for the Mars Surveyor Program 2001 (MSP 01) landed mission is to collect and characterize 91 rock and 13 soil core samples using an integrated instrument suite onboard the Athena Rover. If possible, these samples will be retrieved and returned to Earth via a MSP 05 or MSP 07 mission. Preliminary engineering constraints for the MSP 01 landing site call for a location that lies between 15 S and 30 N, and below about 2 km elevation (based on Viking-era topography). Desirable landing sites for MSP 01 are to be located "in the ancient highlands where the environmental conditions may have been favorable to the preservation of evidence of possible prebiotic or biotic processes including the emergence (and, potentially, the persistence) of life". We interpret this to mean that the desirable sites include those that have evidence of aqueous sediments that might have been deposited during the Noachian and/or Hesperian Epochs of Mars' history. In addition to the search for subaqueous sedimentary deposits, we took into consideration the fact that the rover, Athena, will need to be able to access these materials. Thus, a site where aeolian deflation has occurred might be desirable because it might expose, in situ, layered sedimentary deposits. Deflated areas, of course, might include potential landing hazards in the form of meterscale buttes and mesas (e.g., Christmas Lake Valley, OR), thus careful study of such sites with high resolution images will be required before a decision is made to land. We have been examining three regions that have potential to be considered for MSP 01 landing sites. This work is based on Viking (VIS, IRTM) and Phobos 2 (Termoskan) observations and should be regarded as preliminary because we believe that the final site selection should also be based upon analysis of Mars Global Surveyor observations that help constrain mineralogy (TES) and local geomorphology (MOC, MOLA). (1) Eastern Sinus Meridiani Region (proposed by K. S. Edgett) Sinus Meridiani is a persistent low-albedo (< 0. 16) region on the martian equator that has been recognized for about 400 years. All of Sinus Meridiani is below the 2 km elevation constraint for MSP 01. The region includes cratered highlands, valley networks, aeolian dunes, and possible aqueous sedimentary deposits. The landing site study region is located in the eastern portion of Sinus Meridiani. It is bounded by latitudes 10 S to 2 N, longitudes 355 W to 345 W, and the elevations are mostly 1-2 km. The center of this area contains a medium-albedo (0.19-0.21), relatively smooth-surfaced deposit that was suggested by Rice to be a lacustrine deposit. Several potential landing areas can be suggested within this region. Based on Viking and Phobos 2 data, the favored sites so far are centered at 7.6 S, 346.9 W and 0.8 S, 349 W. Thermal inertias are 3.2-7.0 x 10(exp -3) cal /sq cm s(exp -0.5)/K; and rock abundances are around 2-6%. The site at 7.6 S, 346.9 W is at the southern end of the smooth, medium-albedo unit that might have a lacustrine origin. At this location, numerous channels appear to have drained toward the smooth unit. Viking images from orbit 747A show this area at about 15 m/pixel ground resolution. The images reveal that aeolian deflation has occurred along the deposit's margins. Bright (i.e., albedo >= 0.21) aeolian dunes are present on the channel floors and in some of the depressions on the smooth unit. The bright, apparently active dunes might consist of material (perhaps lakedeposited sands) that has been eroded from the smooth unit. The site at approximately 0.8 S, 349 W is selected because it offers an opportunity to solve a long-standing puzzle about Mars remote sensing. There are three main "color" units on Mars: "dark red, dark gray, and bright red". This landing site would allow the Athena rover an opportunity to investigate all three materials within close proximity (the best place on Mars to do so). There are no high resolution (better than 100 m/pixel) Viking or Mariner images of this site. (2) Central Sinus Meridiani Region (proposed by K. S. Edgett and T. J. Parker) Central Sinus Meridiani is characterized by two types of surfaces [4]. One is like typical martian cratered highlands elsewhere- there are old valley networks and old impact craters. The other is relatively smooth and flat. These two units are in contact around 3.1 S between 5 W and 4 E longitudes. Valley networks- including one at 6'S, 358 W that rivals the Grand Canyon of Arizona- once drained toward the smooth unit. Edgett and Parker [4] proposed that the smooth unit might consist of sediments laid down in a large Noachian-aged sea/ocean that would have covered much of the northern hemisphere. Schultz and Lutz [I I I suggested that it is a paleopolar layered deposit. Regardless, the smooth unit where it contacts the cratered terrain would make an excellent site for Athena rover to investigate. The site is best seen in Viking high resolution images from orbits 408B (about 30 m/pixel) and 746A (about 12 m/pixel). These images suggest that aeolian deflation has occurred along the margin of the smooth unit, and this deflation has exposed horizontal larrs of material. The elevation is about 0.5 km; thermal inertias are 6.5-8.0 x 10(exp -3) cal /sq cm s(exp -0.5) / K; rock abundances are 2-4%; and the surface is probably sandy with dark drifts and ripples but almost no actual dunes. We suggest a landing around 3.2 S, 3.0 W would test the aqueous sediment hypothesis and provide a potentially smooth surface on which to land. (3) Amenthes Fossae Region (proposed by S. N. Huntwork and K. S. Edgett) The Amenthes Fossae are a series of graben/fissures that are circumferential to the southeast side of Isidis Planitia. These fissures cross a variety of ancient, heavily cratered Noachian terrain and younger, Hesperian and Amazonian terrain. We focused our search on a region 0-15 N, 250 - 270 W. Elevations are -0.5 to 2 km. Depending upon whether Isidis Planitia was ever a water-rich environment, this region might have been influenced by aqueous sedimentation. Valley networks are common, and they drained toward the north and northwest. We focused our work on a set of Viking orbiter high-resolution images, 719A 1-48. These have resolutions 16-24 rri/pixel. We examined images 20-23, centered at 2 N, 258 W. This site, on the plains just southwest of a 42 km-diameter crater, includes a valley network channel, a relatively young crater ejecta deposit, a few buttes composed of presumably ancient, Noachian bedrock, and a "plains" unit. The plains might make an ideal landing surface, except for the presence of some fine-scale ridges (oriented approximately N-S). The ridges are probably yardangs, thus this site offers a place where aeolian deflation has probably exposed some of the layered rock units that comprise the "plains". Thermal inertias are 7.9-8.3 x 10(exp -3) cal/sq cm s(exp -0.5) /K and rock abundances are 10-15%. A rover traverse might include the opportunity to go down to the floor (and sample along the walls) of the valley network channel at 2 N 258.1 W.

Edgett, K. S.↗

Subseasonal Clustering of Atmospheric Rivers Over the Western United States

Abstract The serial occurrence of atmospheric rivers (ARs) along the US West Coast can lead to prolonged and exacerbated hydrologic impacts, threatening flood‐control and water‐supply infrastructure due to soil saturation and diminished recovery time between storms. Here a statistical approach for quantifying subseasonal temporal clustering among extreme events is applied to a 41‐year (1979–2019) wintertime AR catalog across the western United States (US). Observed AR occurrence, compared against a randomly distributed AR timeseries with the same average event density, reveals temporal clustering at a greater‐than‐random rate across the western US with a distinct geographical pattern. Compared to the Pacific Northwest, significant AR clusters over the northern Coastal Range of California and Sierra Nevada are more frequent and occur over longer time periods. Clusters along the California Coastal Range typically persist for 2 weeks, are composed of 4–5 ARs per cluster, and account for over 85% of total AR occurrence. Across the northwest Coast‐Cascade Ranges, clusters account for ∼50% of total AR occurrence, typically last 8–10 days, and contain 3–4 individual AR events. Based on precipitation data from a high‐resolution dynamical downscaling of reanalysis, the fractions of total and extreme hourly precipitation attributable to AR clusters are largest along the northern California coast and in the Sierra Nevada. Interannual variability among clusters highlights their importance for determining whether a particular water year is anomalously wet or dry. The mechanisms behind this unusual clustering are unclear and require further research.

Meteorology & Atmospheric Sciences↗

Arbuscular mycorrhizal fungal response to fire and urbanization in the Great Smoky Mountains National Park

Wildfires are increasing in frequency and intensity as drier and warmer climates increase plant detrital fuel loads. At the same time, increases in urbanization position 9% of fire-prone land within the United States at the wildland–urban interface. While rarely studied, the compounded effects of urbanization and wildfires may have unknown synergistically negative effects on ecosystems. Previous studies at the wildland–urban interface often focus on aboveground plant communities, but belowground ecosystems may also be affected by this double disturbance. In particular, it is unclear how much fire and urbanization independently or interactively affect nutritional symbioses such as those between arbuscular mycorrhizal (AM) fungi and the majority of terrestrial plants. In November 2016, extreme drought conditions and long-term fire suppression combined to create a wildfire within the Great Smoky Mountains National Park and the neighboring exurban city of Gatlinburg, TN. To understand how the double disturbance of urbanization and fire affected AM fungal communities, we collected fine roots from the 5 dominant understory species in September 2018 at each of 18 sites spanning 3 burn severities in both exurban and natural sites. Despite large variation in burn severity, plant species identity had the largest influence on AM fungi. AM fungal colonization, richness, and composition all varied most among plant species. Fire and urbanization did influence some AM fungal metrics; colonization was lower in burned sites and composition was more variable among exurban locations. There were no interactions among burn severity and urbanization on AM fungi. Our results point to the large influence of plant species identity structuring this obligate nutritional symbiosis regardless of disturbance regime. Therefore, the majority of AM fungal taxa may be buffered from fire-induced ecosystem changes if plant community composition largely remains intact, plant species life history traits allow for AM fungal persistence after fire disturbance, and/or nearby undisturbed habitat can act as an inoculum source for recolonization following fires. Thus, it is critical to maintain natural, undisturbed habitats interspersed within the wildland–urban interface.

59 BASIC BIOLOGICAL SCIENCES↗

Erosion Rates at the Mars Exploration Rover Landing Sites and Long-Term Climate Change on Mars

Erosion rates derived from the Gusev cratered plains and the erosion of weak sulfates by saltating sand at Meridiani Planum are so slow that they argue that the present dry and desiccating environment has persisted since the Early Hesperian. In contrast, sedimentary rocks at Meridiani formed in the presence of groundwater and occasional surface water, and many Columbia Hills rocks at Gusev underwent aqueous alteration during the Late Noachian, approximately coeval with a wide variety of geomorphic indicators that indicate a wetter and likely warmer environment. Two-toned rocks, elevated ventifacts, and perched and undercut rocks indicate localized deflation of the Gusev plains and deposition of an equivalent amount of sediment into craters to form hollows, suggesting average erosion rates of approx.0.03 nm/yr. Erosion of Hesperian craters, modification of Late Amazonian craters, and the concentration of hematite concretions in the soils of Meridiani yield slightly higher average erosion rates of 1-10 nm/yr in the Amazonian. These erosion rates are 2-5 orders of magnitude lower than the slowest continental denudation rates on Earth, indicating that liquid water was not an active erosional agent. Erosion rates for Meridiani just before deposition of the sulfate-rich sediments and other eroded Noachian areas are comparable with slow denudation rates on Earth that are dominated by liquid water. Available data suggest the climate change at the landing sites from wet and likely warm to dry and desiccating occurred sometime between the Late Noachian and the beginning of the Late Hesperian (3.7-3.5 Ga).

weathering↗

Extending the Reach of IGSN Beyond Earth: Implementing IGSN Registration to Link Nasa's Apollo Lunar Samples and Their Data

The rock and soil samples returned from the Apollo missions from 1969-72 have supported 46 years of research leading to advances in our understanding of the formation and evolution of the inner Solar System. NASA has been engaged in several initiatives that aim to restore, digitize, and make available to the public existing published and unpublished research data for the Apollo samples. One of these initiatives is a collaboration with IEDA (Interdisciplinary Earth Data Alliance) to develop MoonDB, a lunar geochemical database modeled after PetDB (Petrological Database of the Ocean Floor). In support of this initiative, NASA has adopted the use of IGSN (International Geo Sample Number) to generate persistent, unique identifiers for lunar samples that scientists can use when publishing research data. To facilitate the IGSN registration of the original 2,200 samples and over 120,000 subdivided samples, NASA has developed an application that retrieves sample metadata from the Lunar Curation Database and uses the SESAR API to automate the generation of IGSNs and registration of samples into SESAR (System for Earth Sample Registration). This presentation will describe the work done by NASA to map existing sample metadata to the IGSN metadata and integrate the IGSN registration process into the sample curation workflow, the lessons learned from this effort, and how this work can be extended in the future to help deal with the registration of large numbers of samples.

Todd, Nancy S.↗

Synthetic microbial communities: Bridging research and application in second-generation bioenergy feedstock microbiomes

The sustainable production of purpose-grown bioenergy feedstocks is essential in transitioning away from fossil fuels. Synthetic communities (SynComs) are consortia of microorganisms that can be used as biological interventions to support objectives like plant growth and stress tolerance. This review examines the state of knowledge regarding microbiomes and SynComs of second-generation bioenergy feedstocks, focusing on the rhizosphere. We first provide an overview of second-generation feedstocks, including switchgrass (Panicum virgatum), miscanthus (Miscanthus × giganteus), sorghum (Sorghum spp.), sugarcane (Saccharum spp.), and poplar (Populus spp.), and summarize our current understanding of their plant-soil-microbiome ecology. We next discuss considerations in the objectives, design, and evaluation of SynComs to enhance feedstock production, and then critically review the literature around their use. Our literature analysis revealed that SynCom performance varied substantially between controlled pilot experiments and field trials, possibly due to system complexity that could not be fully considered in their design and pilot evaluation. We identified a gap in the use of SynComs to support the unique sustainability objectives of biofuel feedstock agriculture, presenting an opportunity to leverage these additional microbial traits in SynCom designs. Finally, we emphasize the importance of targeted research to identify the ecological principles that govern the assembly, activation, and persistence of microbes in the feedstock rhizosphere, thereby enhancing our capacity to manage microbiomes under diverse environmental conditions and ensure their functionality. Beyond biofuels, SynComs are a promising microbiome management strategy for crop production; however, an ecologically informed design and evaluation of SynComs are advised.

SynCom↗

Influence of contrasting redox conditions on iron (oxyhydr)oxide transformation and associated phosphate sorption

Iron (oxyhydr)oxides strongly adsorb phosphate and limit its bioavailability, but interactions between phosphate and various Fe (oxyhydr)oxides are poorly constrained in natural systems. An in-situ incubation experiment was conducted to explore Fe (oxyhydr)oxide transformation and effects on phosphate sorption in soils with contrasting saturation and redox conditions. Synthetic Fe (oxyhydr)oxides (ferrihydrite, goethite and hematite) were coated onto quartz sand and either pre-sorbed with phosphate or left phosphate-free. The oxide-coated sands were mixed with natural organic matter, enclosed in mesh bags, and buried in and around a vernal pond for up to 12 weeks. Redox conditions were stable and oxic in the upland soils surrounding the vernal pond but largely shifted from Fe reducing to Fe oxidizing in the lowland soils within the vernal pond as it dried during the summer. Iron (oxyhydr)oxides lost more Fe (- 41% ± 10%) and P (- 43 ± 11%) when incubated in the redox-dynamic lowlands compared to the uplands (- 18% ± 5% Fe and - 24 ± 8% P). Averaged across both uplands and lowlands, Fe losses from crystalline goethite and hematite (- 38% ± 6%) were unexpectedly higher than losses from short range ordered ferrihydrite (- 12% ± 10%). We attribute losses of Fe and associated P from goethite and hematite to colloid detachment and dispersion but losses from ferrihydrite to reductive dissolution. Iron losses were partially offset by retention of solubilized Fe as organic-bound Fe(III). Iron (oxyhydr)oxides that persisted during the incubation retained or even gained P, indicating low amounts of phosphate sorption from solution. In conclusion, these results demonstrate that hydrologic variability and Fe (oxyhydr)oxide mineralogy impact Fe mobilization pathways that may regulate phosphate bioavailability.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Impact of Optimized land Surface Parameters on the Land-Atmosphere Coupling in WRF Simulations of Dry and Wet Extremes

Land-atmosphere (L-A) interactions play a critical role in determining the diurnal evolution of both planetary boundary layer (PBL) and land surface temperature and moisture budgets, as well as controlling feedbacks with clouds and precipitation that lead to the persistence of dry and wet regimes. Recent efforts to quantify the strength of L-A coupling in prediction models have produced diagnostics that integrate across both the land and PBL components of the system. In this study, we examine the impact of improved specification of land surface states, anomalies, and fluxes on coupled WRF forecasts during the summers of extreme dry (2006) and wet (2007) conditions in the U.S. Southern Great Plains. The improved land initialization and surface flux parameterizations are obtained through the use of a new optimization and uncertainty module in NASA's Land Information System (LIS-OPT), whereby parameter sets are calibrated in the Noah land surface model and classified according to the land cover and soil type mapping of the observations and the full domain. The impact of the calibrated parameters on the a) spin up of land surface states used as initial conditions, and b) heat and moisture fluxes of the coupled (LIS-WRF) simulations are then assessed in terms of ambient weather, PBL budgets, and precipitation along with L-A coupling diagnostics. In addition, the sensitivity of this approach to the period of calibration (dry, wet, normal) is investigated. Finally, tradeoffs of computational tractability and scientific validity (e.g.,. relating to the representation of the spatial dependence of parameters) and the feasibility of calibrating to multiple observational datasets are also discussed.

Kumar, Sujay↗