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At least 307 records · Page 17

Generation of uniform chromaticity scale imagery from LANDSAT data

An algorithm is presented for generating uniform chromaticity scale (UCS) imagery from multispectral data. A computer program was written to implement the algorithm, and UCS film products were generated. The colors in the film and their temporal change are consistent with those expected for the particular scaling of Krauth components into the (lab) color space. The film product was not subjected to the practical test of competing with previous transformations. Preliminary examination indicates that the product offers the following possibilities: (1) a single film product that will supplant two film products in current use; (2) improved visibility of data differences in regions in data space that are critical to crop identification; and (3) an analytic route to the determination of data-space transformations that will be optimal for particular discrimination problems.

Juday, R. D.↗

The Role of Plants in Space Exploration: Some History and Background

For over 3 decades, NASA has sponsored research on crops for human life support in space. Specialized watering techniques have even been tested for weightless settings, but most studies used conventional watering, such as hydroponics, which should work well on surface settings of the Moon or Mars. NASAs testing has spanned a wide range of crops and studied innovative techniques to increase yields, reduce power, minimize growing volume, and recycle water and nutrients. These issues closely parallel challenges faced in terrestrial controlled environment agriculture, which is expanding around the world.

Bioregenerative↗

Remote Plant Food Production Capability X-Hab

As human space exploration expands to long-duration missions, plant production capability serves as a solution to reduce the mass of consumables necessary to support human metabolic requirements and promote positive crew psychological health. A capability to remotely grow plants for a long-duration space mission could allow crops to be produced before a crew arrives at a staged habitat and could enable plant health to be maintained through remote operation with minimal local crew support. The University of Colorado Boulder was awarded a grant in the summer of 2012 to build a system capable of remote plant production as part of the NASA and National Space Grant Foundation eXploration Habitat (X-Hab) 2013 Academic Innovation Challenge. This report is presented as a review of the Robotic Gardening System that was designed and developed to meet the requirements outlined in the challenge. Each subsystem of the Robotic Gardening System is presented in detail. Included in this report are the design methodologies used, lessons learned throughout the design and development processes, and options or ideas for continued development and future upgrades. In addition to being a review of the Robotic Gardening System and its subsystems, this document is meant to aid with the continued development of this system in conjunction with other projects, such as team member Heather Hava’s NASA Space Technology Research Fellowship (NSTRF) work, X-Hab 2013-14 Plants Anywhere: Growing Plants in Free Habitat Spaces, and Dr. Nikolaus Correll’s NASA Early Career Professionals Fellowship.

Daniel Zukowski↗

Processing of nutritious, safe and acceptable foods from CELSS candidate crops

A controlled ecological life-support system (CELSS) is required to sustain life for long-duration space missions. The challenge is preparing a wide variety of tasty, familiar, and nutritious foods from CELSS candidate crops under space environmental conditions. Conventional food processing technologies will have to be modified to adapt to the space environment. Extrusion is one of the processes being examined as a means of converting raw plant biomass into familiar foods. A nutrition-improved pasta has been developed using cowpea as a replacement for a portion of the durum semolina. A freeze-drying system that simulates the space conditions has also been developed. Other technologies that would fulfill the requirements of a CELSS will also be addressed.

NASA Discipline Number 93-10↗

NASA's Growing Commitment: The Space Garden

Astronauts cannot live on dehydrated ice cream alone. Like everyone else, they need their vegetables. Enter VEGGIE, the Deployable Vegetable System, currently under development by Orbital Technologies Corporation (ORBITEC). VEGGIE is the latest in a long line of vegetable production units ORBITEC is currently working on, with NASA assistance, to grow salad crops to supplement prepackaged foods during long stays in space. The primary goal of the VEGGIE project is to provide flight crews with palatable, nutritious, and safe sources of fresh food with minimal volume and operational resources. In addition, ORBITEC recognizes the age-old adage that gardening is good for the soul, and it acknowledges that gardens are beneficial for relaxation and recreation. As evidence, astronauts on the International Space Station (ISS), who often stay for periods of 6 months, have been enjoying plant experiments, which provide them with much missed greenery and can occupy valuable free time with an enjoyable task. VEGGIE is a project that grew out of technology developed by ORBITEC for the Biomass Production System (BPS). The BPS is equivalent in size to a Space Shuttle middeck locker, and provides four plant growth chambers. Each chamber has independent control of temperature, humidity, nutrient and water delivery, lighting, and atmospheric composition. The BPS flew to the ISS in 2002, and astronaut Dan Bursch had positive comments about his interaction with the plants while in orbit. Astronaut Peggy Whitson had similarly positive remarks during the following expedition while she was growing soybeans for another experiment. Whitson reflects on her time in space with the plantings on Expedition 5, "Although it doesn t sound like much, it was really exciting to see something green. I assumed that this was just because I really enjoy plants, but it surprised me that both of my crewmates were just as excited. They wanted photos of themselves with the plants and asked if they could eat some of them, too!" The astronauts did not eat the plants, but these initial experiments gave the researchers information they needed about the basics of growing crops in space.

Source record↗

Report on Advanced Life Support Activities at Kennedy Space Center

Plant studies at Kennedy Space Center last year focused on selecting cultivars of lettuce, tomato, and pepper for further testing as crops for near-term space flight applications. Other testing continued with lettuce, onion, and radish plants grown at different combinations of light (PPF), temperature, and CO2 concentration. In addition, comparisons of mixed versus mono culture approaches for vegetable production were studied. Water processing testing focused on the development and testing of a rotating membrane bioreactor to increase oxygen diffusion levels for reducing total organic carbon levels and promoting nitrification. Other testing continued to study composting testing for food wastes (NRA grant) and the use of supplemental green light with red/blue LED lighting systems for plant production (NRC fellowship).

Wheeler, Raymond M.↗

Continued Environmental Microbiology Monitoring of the International Space Station (ISS) Veggie Unit Used for In-Flight, Crop-Based Food Systems

Crewmembers live and work in a closed environment that is monitored to ensure their health and safety. To ensure occupants’ health and safety during their spaceflight residency, Environmental Health System (EHS) microbial samples including air, surface, and water, are collected, enumerated, and analyzed quarterly to monitor on-board system contamination and potential risks to crew health. Quarterly monitoring of the microorganisms in the ISS environment supports crew safety and contributes to a large set of microbial concentration and diversity data. Based upon data historically collected over the years, in-flight microbial requirements have been established to maintain the health and safety of the spacecraft environment. This study leverages quarterly operational Environmental Health System (EHS) sampling by collecting additional microbial samples from the surface of the station’s Veggie plant production system. Microbial surface samples collected from the Veggie plant production system will yield microbial concentration and diversity that can be compared and analyzed with nominal surface samples from the vehicle. The data collected in this study will aid in the development of requirements for spaceflight-based food production systems. Continued surface sampling of the internal and external surfaces of the Veggie locker, along with collaboration from both Johnson Space Center (JSC) & Kennedy Space Center (KSC) scientists studying the microbiome of the veggie-crop systems, will be implemented as part of the future development of crop-based food system requirements for the ISS and beyond. This presentation will include a review of the study procedures and evaluations of the current results.

Christian Mena↗

Separation of man-made and natural patterns in high-altitude imagery of agricultural areas

A nonstationary linear digital filter is designed and implemented which extracts the natural features from high-altitude imagery of agricultural areas. Essentially, from an original image a new image is created which displays information related to soil properties, drainage patterns, crop disease, and other natural phenomena, and contains no information about crop type or row spacing. A model is developed to express the recorded brightness in a narrow-band image in terms of man-made and natural contributions and which describes statistically the spatial properties of each. The form of the minimum mean-square error linear filter for estimation of the natural component of the scene is derived and a suboptimal filter is implemented. Nonstationarity of the two-dimensional random processes contained in the model requires a unique technique for deriving the optimum filter. Finally, the filter depends on knowledge of field boundaries. An algorithm for boundary location is proposed, discussed, and implemented.

Samulon, A. S.↗

Significance of vegetation in interpreting thermal radiation from a terrestrial surface

The regions between crop rows are modeled as infinitely long diffuse cavities. Geometrical view factors are calculated, accounting for reflected radiation emanating internal to the cavity and from the background sky. A 'cavity' emissivity dependent on local geometry is defined to allow for correcting apparent temperatures to true temperatures. The total emissivity appropriate for correcting aircraft thermal data is then calculated by averaging all emissivity components to account for soil, crop, and background. It is shown that even for the lowest crop and vegetation emissivities, if the crop height-to-spacing ratio is greater than unity, the composite emissivity is greatly increased, giving rise to a decreased error in remotely sensed temperature data.

Sutherland, R. A.↗

Microbiological Horticultural Internship Final Abstract

GMO dwarf plum (Prunus domestica) is being evaluated as a candidate food crop for long duration space flight missions. A project was undertaken to develop a protocol for transferring selected genetic lines of GMO plum (previously maintained in pots and propagated by cuttings at NASA's Kennedy Space Center in Florida) into in vitro tissue culture. In vitro culture may reduce the space, materials, and labor required to maintain the current lines of GMO plum and better preserve them for future study. Fresh plant material from three selected GMO plum lines (NASA-5, NASA-10, and NASA-11) and a non-modified control line (Control-5) were processed aseptically into in vitro culture on four separate occasions. The impact of multiple treatments on the successful growth of GMO plum tissue in vitro were tested: Parent explant tissue type (leaf petioles, stem nodes containing buds and internodes without buds), tissue sterilization method [soaking in 10 bleach only (5 min for petioles or 10 min for nodesinternodes), or soaking in 70 EtOH (30 sec) followed by 10 bleach (5 min for petioles and 10 min for nodesinternodes)], and media type [three Murashige and Skoog-based medias (SGM, SRM, and SRM+2,4-D) and one recipe containing woody plant media (WPM)]. 22.2 of the plates containing tissue sterilized with bleach alone developed microbial contamination after two weeks, while only 11.8 of plates containing tissue sterilized sequentially with EtOH and bleach developed contamination. Node bud tissue from all four genetic lines of plum produced leafy plantlets on SGM and SRM media after 4-6 weeks. The most numerous and well-developed plantlets were present on SGM. Upon reaching suitable size, plantlets were transferred to larger media containers for further growth. Some node bud growth occurred on SRM+2,4-D and WPM 2.5 weeks after plating, however as of yet no pieces on SRM+2,4-D have adequate development for transferring. Tissue pieces from NASA-5 plated on WPM are developing leaves and will be ready for transferring soon. Petioles and internode tissue lacking bud meristem failed to produce any plantlets on any plates, however petioles developed large masses of undifferentiated callus tissue on SRM+2,4-D media. These callused pieces were then transferred to SRM+TDZ media, which resulted in even larger callus growth but no differentiation. All four selected plum lines were successfully transitioned into in vitro culture. Nodes from NASA-5 and NASA-10 lines produced the most numerous and well-developed leafy plantlets in vitro, while those from NASA-11 and Control-5 were generally smaller, slower growing and less numerous. The best method overall was to use young stem node tissue with buds, surface sterilize the pieces sequentially with 70 EtOH and 10 bleach, and then plate them onto SGM media. Future areas of study will include introducing additional genetic lines of GMO plum into in vitro culture, attempting to induce shoot growth in petiole callus tissue, testing methods (such as cold storage) that extend the time interval between transferring explants into new media, and testing viability of plantlets transferred from in vitro culture back to traditional pot culture.

bioregenerative life support↗

Foods for a Mission to Mars: Investigations of Low-Dose Gamma Radiation Effects

Food must be safe, nutritious, and acceptable throughout a long duration mission to maintain the health, well-being, and productivity of the astronauts. In addition to a developing a stable pre-packaged food supply, research is required to better understand the ability to convert edible biomass into safe, nutritious, and acceptable food products in a closed system with many restrictions (mass, volume, power, crew time, etc.). An understanding of how storage conditions encountered in a long-term space mission, such as elevated radiation, will impact food quality is also needed. The focus of this project was to contribute to the development of the highest quality food system possible for the duration of a mission, considering shelf-stable extended shelf-life foods, bulk ingredients, and crops to be grown in space. The impacts of space-relevant radiation doses on food, bulk ingredient, and select candidate crop quality and antioxidant capacity were determined. Interestingly, increasing gamma-radiation doses (0 to 1000 Gy) did not always increase dose-related effects in foods. Intermediate radiation doses (10 to 800Gy) often had significantly larger impact on the stability of bulk ingredient oils than higher (1000Gy) radiation doses. Overall, most food, ingredient, and crop systems investigated showed no significant differences between control samples and those treated with 3 Gy of gamma radiation (the upper limit estimated for a mission to Mars). However, this does not mean that all foods will be stable for 3-5 years, nor does it mean that foods are stable to space radiation comprising more than gamma rays.

Gandolph, J.↗

Control of water and nutrients using a porous tube - A method for growing plants in space

A plant nutrient delivery system that uses a microporous, hydrophilic tube was developed with potential application for crop production in the microgravity of space. The tube contains a nutrient solution and delivers it to the roots. Pumps attached to the tubing create a very small suction that holds the solution within the tube. This system was used to grow wheat for 107 d in a controlled environment at suctions of 0.40, 1.48, or 2.58 kPa. The water absorbed through the pores of the tube by baby diaper sections decreased as suction increased. Correspondingly, final plant biomass, seed number, and spikelet number also tended to decrease as suction increased. The reduced yield at higher suction suggests that the plants experienced water stress, although all suctions were below those typical of soils at field capacity.

Dreschel, Thomas W.↗

Impact of Space Radiation with Other Combined Space Environmental Factors on Plant Seeds and Plant Development: from Arabidopsis thaliana to Crops

One of the major concerns for long-term exploration missions beyond Earth’s magnetosphere is radiation risk, primarily from solar particle events (SPE) and galactic cosmic rays (GCR). With the goal of manned Mars exploration, the production of fresh food during long-duration space missions provides critical nutritional supplementation and may also benefit astronauts’ behavioral health. However, the effects of space radiation on plants and plant propagules have not been sufficiently investigated and characterized. To evaluate the effects of space radiation on plant seeds and development, we explored several platforms, including ground-based radiation facilities, the MISSE platform outside the ISS, and other Low Earth Obit (LEO) opportunities. Radiation particle types ranged from neutrons, single charged particles, simulated galactic cosmic rays, simulated solar particle events, the radiation field outside the ISS (total 10 months on the ISS), and the field inside a LEO vehicle (over 3 years). Radiation doses ranged from around 10 cGy to 80 cGy with acute or chronic exposures. In these studies up to 15 types of model plant and crop seeds were evaluated. In addition, tests were conducted to determine whether the combination of radiation exposure and simulated microgravity could have synergetic effects. Exposure to space radiation at the levels tested had no significant impact on the germination rate in Arabidopsis and the seeds from multiple crop plants; however, radiation exposure alone or with other combined factors reduced the viability of some seed types. Overall, the changes within plants grown from irradiated seeds were dose- and ion quality- dependent, with heavier ions causing more severe damage. These changes ranged from cotyledon deformation, shortened root length, smaller seedling size, and other signs of stress, depending on the seed types. Radiation exposure also significantly altered transcriptomic profiles in seedlings grown from irradiated seeds. The impact of space radiation on seeds and plants potentially affects the ability of plants to adapt to other environmental stresses (e.g. microgravity, water stress, and hardware constraints) as well as susceptibility to plant diseases, which need to be furtherly investigated.

Y Zhang↗

Crop Production for Advanced Life Support Systems - Observations From the Kennedy Space Center Breadboard Project

The use of plants for bioregenerative life support for space missions was first studied by the US Air Force in the 1950s and 1960s. Extensive testing was also conducted from the 1960s through the 1980s by Russian researchers located at the Institute of Biophysics in Krasnoyarsk, Siberia, and the Institute for Biomedical Problems in Moscow. NASA initiated bioregenerative research in the 1960s (e.g., Hydrogenomonas) but this research did not include testing with plants until about 1980, with the start of the Controlled Ecological Life Support System (CELSS) Program. The NASA CELSS research was carried out at universities, private corporations, and NASA field centers, including Kennedy Space Center (KSC). The project at KSC began in 1985 and was called the CELSS Breadboard Project to indicate the capability for plugging in and testing various life support technologies; this name has since been dropped but bioregenerative testing at KSC has continued to the present under the NASA s Advanced Life Support (ALS) Program. A primary objective of the KSC testing was to conduct pre-integration tests with plants (crops) in a large, atmospherically closed test chamber called the Biomass Production Chamber (BPC). Test protocols for the BPC were based on observations and growing procedures developed by university investigators, as well as procedures developed in plant growth chamber studies at KSC. Growth chamber studies to support BPC testing focused on plant responses to different carbon dioxide (CO2) concentrations, different spectral qualities from various electric lamps, and nutrient film hydroponic culture techniques.

Wheeler, R. M.↗

Open Science for Life in Space: Data Sharing and Tools for Knowledge Discovery

The next era in human space exploration is rapidly approaching. The use of health countermeasures and biomonitoring systems for space missions are required to counteract space health hazards and to support life to thrive in deep space (e.g., humans, animals, plants, crops; entire ecosystems within spacecrafts/habitats/spacesuits). The development of these mission components will be highly dependent on our understanding of basic biological and health responses to myriad space hazards (ionizing radiation, altered gravitational fields, altered day-night cycles, confined isolation, hostile-closed environments, distance-duration from Earth, planetary dust-regolith, and extreme temperatures/atmospheres). The fast-growing array of space biological and mission telemetry data, which in the past was simply archived after minimal analysis, holds great potential once applied to these mission challenges if it can be reorganized and formatted for Open Science. Organizing the data for such analysis is a challenge because of its multi-hierarchical, multi-modal, and heterogenous nature (molecular, cellular, tissue, organ, whole organism, behavior, ecosystem, microbiome; tabular, omics, imaging, video, biospecimen, environmental physical-chemical telemetry). This session focuses on current approaches in this domain such as: making space biological data FAIR (findable, accessible, interoperable, reusable), effective data ingestion/dissemination, observational versus experimental data, Open Science collaborations, data analysis techniques, AI/ML/knowledge graph/modeling methods, and data integration/discovery tools.

open science↗

Agribusiness and space: No limits to growth

Technological developments responding to world food needs are examined. It is noted that agribusiness technology has become more space-related in recent years. Although crops forecasting and improvements in yield (the green revolution) were developed prior to the space era, it would be unthinkable today to ignore the contributions of operational meteorological and communications satellites and experimental Earth observation satellites in agribusiness. Space-driven communications now permit national agribusiness database management networks, with a significant portion of the data being space-derived. In demonstration experiments, space communications were shown to improve those aspects of the food problem related to education and communications.

Montgomery, O. L.↗