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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 307 records · Page 17

Fast-RF-Shimming: Accelerate RF shimming in 7T MRI using deep learning

Ultrahigh field (UHF) Magnetic Resonance Imaging (MRI) offers an elevated signal-to-noise ratio (SNR), enabling exceptionally high spatial resolution that benefits both clinical diagnostics and advanced research. However, the jump to higher fields introduces complications, particularly transmit radiofrequency (RF) field ($B^{+}_{1}$) inhomogeneities, manifesting as uneven flip angles and image intensity irregularities. These artifacts can degrade image quality and impede broader clinical adoption. Traditional RF shimming methods, such as Magnitude Least Squares (MLS) optimization, effectively mitigate $B^{+}_{1}$ inhomogeneity, but remain time-consuming. Recent machine learning approaches, including RF Shim Prediction by Iteratively Projected Ridge Regression and other deep learning architectures, suggest alternative pathways. Although these approaches show promise, challenges such as extensive training periods, limited network complexity, and practical data requirements persist. In this paper, we introduce a holistic learning-based framework called Fast-RF-Shimming, which achieves a 5000 ​× ​speed-up compared to the traditional MLS method. In the initial phase, we employ random-initialized Adaptive Moment Estimation (Adam) to derive the desired reference shimming weights from multi-channel $B^{+}_{1}$ fields. Next, we train a Residual Network (ResNet) to map $B^{+}_{1}$ fields directly to the ultimate RF shimming outputs, incorporating the confidence parameter into its loss function. Finally, we design Non-uniformity Field Detector (NFD), an optional post-processing step, to ensure the extreme non-uniform outcomes are identified. Comparative evaluations with standard MLS optimization underscore notable gains in both processing speed and predictive accuracy, which indicates that our technique shows a promising solution for addressing persistent inhomogeneity challenges.

Deep learning↗

Estimating tropical cyclone central pressure and outer winds from satellite microwave data

A new technique is presented for estimating central pressure and surface winds outside of the radius of maximum winds from brightness temperatures measured by the SCAMS on Nimbus 6. The problem of estimating maximum surface winds is not treated because the spatial resolution of the radiometer is not high enough for this purpose. The technique is developed on the basis of measurements over eight typhoons and five hurricanes during 1975. It is shown that the warm 55.45-GHz brightness temperature anomaly previously observed over Typhoon June can be found over other tropical cyclones. Maximum brightness temperature anomaly correlates with central pressure with a correlation coefficient of -0.859. Outer surface wind speeds are calculated by assuming gradient balance and using the regression between brightness temperature anomaly and central pressure to estimate pressure gradients.

Kidder, S. Q.↗

Case studies using GOES infrared data and a planetary boundary layer model to infer regional scale variations in soil moisture

Modeled temperature data from a one-dimensional, time-dependent, initial value, planetary boundary layer model for 16 separate model runs with varying initial values of moisture availability are applied, by the use of a regression equation, to longwave infrared GOES satellite data to infer moisture availability over a regional area in the central U.S. This was done for several days during the summers of 1978 and 1980 where a large gradient in the antecedent precipitation index (API) represented the boundary between a drought area and a region of near normal precipitation. Correlations between satellite derived moisture availability and API were found to exist. Errors from the presence of clouds, water vapor and other spatial inhomogeneities made the use of the measurement for anything except the relative degree of moisture availability dubious.

Rose, F. G.↗

Active layer depth and soil properties impact specific leaf area variation and ecosystem productivity in a boreal forest

Specific leaf area (SLA, leaf area per unit dry mass) is a key canopy structural characteristic, a measure of photosynthetic capacity, and an important input into many terrestrial process models. Although many studies have examined SLA variation, relatively few data exist from high latitude, climate-sensitive permafrost regions. We measured SLA and soil and topographic properties across a boreal forest permafrost transition, in which dominant tree species changed as permafrost deepened from 54 to >150 cm over 75 m hillslope transects in Caribou-Poker Creeks Research Watershed, Alaska. We characterized both linear and threshold relationships between topographic and edaphic variables and SLA and developed a conceptual model of these relationships. We found that the depth of the soil active layer above permafrost was significantly and positively correlated with SLA for both coniferous and deciduous boreal tree species. Intraspecific SLA variation was associated with a fivefold increase in net primary production, suggesting that changes in active layer depth due to permafrost thaw could strongly influence ecosystem productivity. While this is an exploratory study to begin understanding SLA variation in a non-contiguous permafrost system, our results indicate the need for more extensive evaluation across larger spatial domains. These empirical relationships and associated uncertainty can be incorporated into ecosystem models that use dynamic traits, improving our ability to predict ecosystem-level carbon cycling responses to ongoing climate change.

59 BASIC BIOLOGICAL SCIENCES↗

Optode Images, and Dissolved Oxygen Data from Sand and Sediment Flow-through Column Experiments associated with: "2-D Imaging of Dissolved Oxygen Concentration in Flow-Through Sediment Columns"

This data package is associated with the publication "2-D Imaging of Dissolved Oxygen Concentration in Flow-Through Sediment Columns" submitted to Frontiers in Water (Garayburu-Caruso et al., 2022). This work presents a novel application of planar optode technology to sediment columns that allows the study of well-constrained flow fields without the need to assume completely homogeneous flow. We introduce a flow-through column with the interior coated in an oxygen sensing film and a series of sampling ports as a unique tool capable of capturing changes in dissolved oxygen (DO) at high frequency (10 s) and spatial (< 1 mm) resolution across the length of a column. In addition, we provide custom scripts that allows image processing steps to be automated and consistently reproduced over a variety of experimental conditions. This dataset is comprised of four folders (1) 01_Riverbed_Sediment_Experiment, (2) 02_Sand_Experiment, (3) 01_Riverbed_Sediment_Raw_Images, and (4) 02_Sand_Experiment_Raw_Images. 01_Riverbed_Sediment_Experiment contains (1) a subfolder with csv files that contain statistical parameters from the linear regressions applied to each optode image, (2) a subfolder with csv files from DO data measured by in-line sensors, (3) a subfolder with csv files containing column and injection specific input parameters for the image processing script, and (4) a subfolder with scripts used to process the images and create associated figures. 02_Sand_Experiment contains (1) a subfolder with csv files containing column and injection-specific input parameters for the image processing script, and (2) a subfolder with scripts used to process the images and create associated figures. 01_Riverbed_Sediment_Raw_Images (spited in 3 parts due to size) contains a subfolder with raw red, and green images from the optode system during the MilliQ Injection, Vanillin Injection or Vanillin + Sampling Injection respectively. 02_Sand_Experiment_Raw_Images contains a subfolder with raw red, and green images from the optode system. Outside of the main folders there is a csv containing file-level metadata and a csv data dictionary defining column headers for all csv files contained in the data package.

54 ENVIRONMENTAL SCIENCES↗

Relationship between selected orientation rest frame, circular vection and space motion sickness

Space motion sickness (SMS) and spatial orientation and motion perception disturbances occur in 70-80% of astronauts. People select "rest frames" to create the subjective sense of spatial orientation. In microgravity, the astronaut's rest frame may be based on visual scene polarity cues and on the internal head and body z axis (vertical body axis). The data reported here address the following question: Can an astronaut's orientation rest frame be related and described by other variables including circular vection response latencies and space motion sickness? The astronaut's microgravity spatial orientation rest frames were determined from inflight and postflight verbal reports. Circular vection responses were elicited by rotating a virtual room continuously at 35 degrees/s in pitch, roll and yaw with respect to the astronaut. Latency to the onset of vection was recorded from the time the crew member opened their eyes to the onset of vection. The astronauts who used visual cues exhibited significantly shorter vection latencies than those who used internal z axis cues. A negative binomial regression model was used to represent the observed total SMS symptom scores for each subject for each flight day. Orientation reference type had a significant effect, resulting in an estimated three-fold increase in the expected motion sickness score on flight day 1 for astronauts who used visual cues. The results demonstrate meaningful classification of astronauts' rest frames and their relationships to sensitivity to circular vection and SMS. Thus, it may be possible to use vection latencies to predict SMS severity and duration.

short duration↗

Primordial non-Gaussianity from the completed SDSS-IV extended Baryon Oscillation Spectroscopic Survey – I: Catalogue preparation and systematic mitigation

ABSTRACT We investigate the large-scale clustering of the final spectroscopic sample of quasars from the recently completed extended Baryon Oscillation Spectroscopic Survey (eBOSS). The sample contains 343 708 objects in the redshift range 0.8 < z < 2.2 and 72 667 objects with redshifts 2.2 < z < 3.5, covering an effective area of $4699\, {\rm deg}^{2}$. We develop a neural network-based approach to mitigate spurious fluctuations in the density field caused by spatial variations in the quality of the imaging data used to select targets for follow-up spectroscopy. Simulations are used with the same angular and radial distributions as the real data to estimate covariance matrices, perform error analyses, and assess residual systematic uncertainties. We measure the mean density contrast and cross-correlations of the eBOSS quasars against maps of potential sources of imaging systematics to address algorithm effectiveness, finding that the neural network-based approach outperforms standard linear regression. Stellar density is one of the most important sources of spurious fluctuations, and a new template constructed using data from the Gaia spacecraft provides the best match to the observed quasar clustering. The end-product from this work is a new value-added quasar catalogue with the improved weights to correct for non-linear imaging systematic effects, which will be made public. Our quasar catalogue is used to measure the local-type primordial non-Gaussianity in a companion paper.

79 ASTRONOMY AND ASTROPHYSICS↗

Improved Rainfall Data in the Philippines through Concurrent Use of GPM IMERG and Ground-Based Measurements

The availability of accurate and reliable rainfall data that are applicable to various phenomenological, climatological, and modeling studies is important, especially in the Philippines, which is considered to be highly vulnerable to natural hazards and a changing climate. The presented strategy involved constructing a dataset consisting of synoptic data, automatic rain gauge (ARG) measurements, and satellite data that are co-registered, consistent, and formatted in the same manner. Although sparse in number, the synoptic stations provide the most accurate rainfall information and were used as the baseline for creating the dataset. The ARGs that are within a distance of 1 km to the synoptic stations were used to determine the correction factors needed to make the synoptic and ARG data consistent. Subsequently, the corrected ARGs were used to make the satellite IMERG data consistent with both ARG and synoptic data. In case of the latter, only IMERG pixels with at least 10 ARGs within the relatively large footprint of the satellite sensor were used in estimating the required correction parameters derived from a combination of a power transform and linear regression correction techniques. The final results show good agreement of synoptic and corrected ARG data with correlation coefficients of 0.94 and 0.97 for the 10 day and monthly data, respectively, and improvement in the linear regression slope from 0.67 to 0.90 for 10 day data, and 0.70 to 0.94 for monthly data. In addition, the corrected ARG data agree well with the corrected IMERG data, with correlation coefficients of 0.88 and 0.93 for the 10 day and monthly data, respectively, and an improvement in slope from 0.66 to 0.87 for 10 day data, and 0.74 to 0.99 for monthly data. The merit of using a combined dataset is illustrated through comparative analyses of the IMERG data and spatially interpolated synoptic and ARG data. The results show general agreements in spatial patterns of rainfall across the datasets, especially in areas where in situ measurements are recorded. The observed discrepancy when ground data is limited emphasizes the need for satellite IMERG data to obtain the true spatial patterns of rainfall distribution.

rainfall↗

Effects of fuel composition and octane sensitivity on polycyclic aromatic hydrocarbon and soot emissions of gasoline–ethanol blend surrogates

The sooting propensity of a fuel is closely coupled with the fuel composition and chemistry. A detailed understanding of their effects is, therefore, needed to develop next-generation fuels which can minimize particulate emissions. With this overarching goal, the present work numerically investigates the effects of fuel composition and octane sensitivity (S) on polycyclic aromatic hydrocarbons (PAH) and soot emissions, for four-component gasoline-ethanol blend surrogates comprising isooctane, n-heptane, toluene, and ethanol. A partially-premixed counterflow flame is chosen as the canonical configuration for this study and simulations are performed using CHEMKIN-Pro-employing a kinetic mechanism developed by Park et al. (2017). In addition, a, detailed soot model based on the sectional method is used to capture the spatial characteristics of soot emissions. The kinetic mechanism and soot model are validated using available experimental data for various targets. A total of 86 TPRF-E mixtures, spanning a wide range of concentration of each component, and a wide range of S are analyzed. The effect of each non-paraffinic fuel component on the resultant PAH and soot emissions is investigated. PAH and soot emissions are found to vary significantly depending upon the blend composition. Additionally, based on the parametric sweeps, a regression analysis is carried out to identify global parameters that govern the formation of PAHs and soot. The analysis shows that both toluene content and S have a prominent effect on the formation of PAHs and soot, with toluene content having a stronger impact. Moreover, larger PAHs have higher dependency on toluene content and S. Furthermore, a detailed analysis is carried out to understand the physical and chemical phenomena associated with the observed trends of PAH and soot emissions.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Resolving the fast ion distribution from imaging neutral particle analyzer measurements

A recently developed imaging neutral particle analyzer (INPA) on the DIII-D tokamak (Du 2018 Nucl. Fusion 58 082006) enables fast ion velocity-space tomography of high fidelity at the interrogated phase space. To accomplish this, the spatial and energy depending fast ( E < 80 keV) neutral flux towards the INPA stripping foils is calculated with FIDASIM and a newly developed code INPASIM simulates the INPA instrumental response to this neutral flux. Included in INPASIM is the neutral-foil interaction, the Larmor orbit tracing between the foil and the phosphor, the phosphor response to the incident ion flux as well as camera focusing. Benefiting from heavy, localized velocity-space weights and excellent signal to noise, computed tomography using the Ridge regression method is able to successfully reconstruct fine-scale velocity-space structures produced by multiple neutral beams separated by as small as ~3 keV in tests. Applying the inversion method to a sawtooth crash event reveals a significant profile flattening of confined passing particles across q = 1 flux surface, as well as a redistribution of fast ions into the trapped orbits at the plasma edge close to the last closed flux surface.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Evaluation of quantitative synchrotron radiation micro-X-ray fluorescence in rice grain

Concentrations of nutrients and contaminants in rice grain affect human health, specifically through the localization and chemical form of elements. Methods to spatially quantify the concentration and speciation of elements are needed to protect human health and characterize elemental homeostasis in plants. Here, an evaluation was carried out using quantitative synchrotron radiation microprobe X-ray fluorescence (SR-µXRF) imaging by comparing average rice grain concentrations of As, Cu, K, Mn, P, S and Zn measured with rice grain concentrations from acid digestion and ICP-MS analysis for 50 grain samples. Better agreement was found between the two methods for high-Z elements. Regression fits between the two methods allowed quantitative concentration maps of the measured elements. These maps revealed that most elements were concentrated in the bran, although S and Zn permeated into the endosperm. Arsenic was highest in the ovular vascular trace (OVT), with concentrations approaching 100 mg kg –1 in the OVT of a grain from a rice plant grown in As-contaminated soil. Quantitative SR-µXRF is a useful approach for comparison across multiple studies but requires careful consideration of sample preparation and beamline characteristics.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

Use of Satellite, Surface Observations and Numerical Weather Prediction Model Data to Improve Cloud Base Height and Cloud Base Vertical Velocity Estimation

Cloud base height (CBH) and cloud base vertical velocity (CBVV) are important variables that impact the overall climate in a region as they influence the formulation, longevity, and evolution of clouds. Retrieval of both parameters have long used ground instrumentation (e.g., Doppler lidar (DL), ground base radar); however, retrieving CBH from satellites is particularly challenging given that space-based instruments only observe cloud tops. In this manuscript, CBH is retrieved using a multi-linear regression equation, while CBVV used a random forests model. Both retrievals combine satellite and numerical weather prediction data. The satellite data used are the Visible Infrared Imaging Radiometer Suite imagery, while measurements of CBH and CBVV include DL and radiosonde data at the Southern Great Plains (SGP) Atmospheric Radiation Measurement observatory. Data from 83 summer days (May-August) in 2018–2021 featuring cumulus clouds forced by solar heating were examined and used to train the models, with years 2022–2023 used for validation. Various spatial domains were defined with one large (2.4° longitude by 2.0° latitude) SGP domain being split into smaller sections (smallest being 0.99° and 0.61° longitude and latitude respectably). CBH and CBVV values obtained from the DL as compared to the models show root mean square errors between 150 and 200 m, with CBVV values between 0.45 and 1 ms -1 . Finally, it was found that the CBH formulation performs well over all domains, while the CBVV retrievals become less accurate due to more turbulence being introduced into the observations as the number of DL stations decreases in the smaller domains.

54 ENVIRONMENTAL SCIENCES↗

Radiation Mapping for an Unmanned Aerial Vehicle: Development and Simulated Testing of Algorithms for Source Mapping and Navigation Path Generation

Image reconstruction algorithms were developed for radiation source mapping and used for generating the search path of a moving radiation detector, such as one onboard an unmanned aerial vehicle. Simulations consisted of first assuming radioactive sources of varying complexity and estimating the radiation fields that would then be produced by that source distribution. Next, the "measurements" that would result from a pair of adjacent spatial locations were computed. A crude estimate of the source distribution likely to have produced such "measurements" was reconstructed based upon the limited measurements. Location of the next "measurement" was then determined as halfway between the location of the estimated source and the current "measurement." With each additional sample, improved source distribution reconstructions were made and used to inform the immediate direction of detector motion. Source reconstruction or mapping was formulated as an inverse problem solved with either maximum a posteriori or least squares (LS) regression deconvolution methods. Different amounts of noise were added to the simulated "measurements," allowing evaluation of the methods' performances as functions of signal-to-noise ratio of the measured map. As expected, methods that promote sparsity were better suited in reconstructing point sources. Reliable prior information of the source distribution also improved the reconstruction results, especially with distributed sources. With a non-negative least square algorithm and the suggested paths it generated, location of sources was successfully estimated to an accuracy of 0.014 m within nine iterations in a single-source scenario and 12 iterations in a two-source scenario, given a 10% error on the integrated counts and a Poisson distribution of the noise associated with the measured counts.

98 NUCLEAR DISARMAMENT, SAFEGUARDS, AND PHYSICAL P↗

The impact of conventional surface data upon VAS regression retrievals in the lower troposphere

Surface temperature and dewpoint reports are added to the infrared radiances from the VISSR Atmospheric Sounder (VAS) in order to improve the retrieval of temperature and moisture profiles in the lower troposphere. The conventional (airways) surface data are combined with the twelve VAS channels as additional predictors in a ridge regression retrieval scheme, with the aim of using all available data to make high resolution space-time interpolations of the radiosonde network. For one day of VAS observations, retrievals using only VAS radiances are compared with retrievals using VAS radiances plus surface data. Temperature retrieval accuracy evaluated at coincident radiosonde sites shows a significant impact within the boundary layer. Dewpoint retrieval accuracy shows a broader improvement within the lowest tropospheric layers. The most dramatic impact of surface data is observed in the improved relative spatial and temporal continuity of low-level fields retrieved over the Midwestern United States.

Lee, T. H.↗

The impact of conventional surface data upon VAS regression retrievals in the lower troposphere

Surface temperature and dewpoint reports are added to the infrared radiances from the VISSR Atmospheric Sounder (VAS) in order to improve the retrieval of temperature and moisture profiles in the lower troposphere. The conventional (airways) surface data are combined with the twelve VAS channels as additional predictors in a ridge regression retrieval scheme, with the aim of using all available data to make high resolution space-time interpolations of the radiosonde network. For one day of VAS observations, retrievals using only VAS radiances are compared with retrievals using VAS radiances plus surface data. Temperature retrieval accuracy evaluated at coincident radiosonde sites shows a significant impact within the boundary layer. Dewpoint retrieval accuracy shows a broader improvement within the lowest tropospheric layers. The most dramatic impact of surface data is observed in the improved relative spatial and temporal continuity of low-level fields retrieved over the Midwestern United States. Previously announced in STAR as N83-27522

Lee, T.-H.↗

Optimizing transmit field inhomogeneity of parallel RF transmit design in 7T MRI using deep learning

Ultrahigh field (UHF) Magnetic Resonance Imaging (MRI) provides a higher signal-to-noise ratio and, thereby, higher spatial resolution. However, UHF MRI introduces challenges such as transmit radiofrequency (RF) field (B+1) inhomogeneities, leading to uneven flip angles and image intensity anomalies. These issues can significantly degrade imaging quality and its medical applications. This study addresses B+1 field homogeneity through a novel deep learning-based strategy. Traditional methods like Magnitude Least Squares (MLS) optimization have been effective but are time-consuming and dependent on the patient’s presence. Recent machine learning approaches, such as RF Shim Prediction by Iteratively Projected Ridge Regression and deep learning frameworks, have shown promise but face limitations like extensive training times and oversimplified architectures. We propose a two-step deep learning strategy. First, we obtain the desired reference RF shimming weights from multi-channel B+1 fields using random-initialized Adaptive Moment Estimation. Then, we employ Residual Networks (ResNets) to train a model that maps B+1 fields to target RF shimming outputs. Our approach does not rely on pre-calculated reference optimizations for the testing process and efficiently learns residual functions. Comparative studies with traditional MLS optimization demonstrate our method’s advantages in terms of speed and accuracy. The proposed strategy achieves a faster and more efficient RF shimming design, significantly improving imaging quality at UHF. This advancement holds potential for broader applications in medical imaging and diagnostics.

Lu, Zhengyi [Vanderbilt University]↗

High Spectral Resolution Lidar – Generation 2 (HSRL-2) Retrievals of Ocean Surface Wind Speed: Methodology and Evaluation

Ocean surface wind speed (i.e., wind speed 10 m above sea level) is a critical parameter used by atmospheric models to estimate the state of the marine atmospheric boundary layer (MABL). Accurate surface wind speed measurements in diverse locations are required to improve characterization of MABL dynamics and assess how models simulate large-scale phenomena related to climate change and global weather patterns. To provide these measurements, this study introduces and evaluates a new surface wind speed data product from the NASA Langley Research Center nadir-viewing High Spectral Resolution Lidar – generation 2 (HSRL-2) using data collected as part of the NASA Aerosol Cloud meTeorology Interactions oVer the western ATlantic Experiment (ACTIVATE) mission. The HSRL-2 can directly measure vertically resolved aerosol backscatter and extinction profiles without additional constraints or assumptions, enabling the instrument to accurately derive atmospheric attenuation and directly determine surface reflectance (i.e., surface backscatter). Also, the high horizontal spatial resolution of the HSRL-2 retrievals (0.5 s or ∼ 75 m along track) allows the instrument to probe the fine-scale spatial variability in surface wind speeds over time along the flight track and over breaks in broken cloud fields. A rigorous evaluation of these retrievals is performed by comparing coincident HSRL-2 and National Center for Atmospheric Research (NCAR) Airborne Vertical Atmosphere Profiling System (AVAPS) dropsonde data, owing to the joint deployment of these two instruments on the ACTIVATE King Air aircraft. These comparisons show correlations of 0.89, slopes of 1.04 and 1.17, and y intercepts of −0.13 and −1.05 m s−1 for linear and bisector regressions, respectively, and the overall accuracy is calculated to be 0.15 ± 1.80 m s−1. It is also shown that the dropsonde surface wind speed data most closely follow the HSRL-2 distribution of wave slope variance using the distribution proposed by Hu et al. (2008) rather than the ones proposed by Cox and Munk (1954) and Wu (1990) for surface wind speeds below 7 m s−1, with this category comprising most of the ACTIVATE data set. The retrievals are then evaluated separately for surface wind speeds below 7 m s−1 and between 7 and 13.3 m s−1 and show that the HSRL-2 retrieves surface wind speeds with a bias of ∼ 0.5 m s−1 and an error of ∼ 1.5 m s−1, a finding not apparent in the cumulative comparisons. Also, it is shown that the HSRL-2 retrievals are more accurate in the summer (−0.18 ± 1.52 m s−1) than in the winter (0.63 ± 2.07 m s−1), but the HSRL-2 is still able to make numerous (N=236) accurate retrievals in the winter. Overall, this study highlights the abilities and assesses the performance of the HSRL-2 surface wind speed retrievals, and it is hoped that further evaluation of these retrievals will be performed using other airborne and satellite data sets.

Sanja Dmitrovic↗

Microbial community dynamics and cycling of plutonium and iron in a seasonally stratified and radiologically contaminated pond

Plutonium (Pu) cycling and mobility in the environment can be impacted by the iron cycle and microbial community dynamics. We investigated the spatial and temporal changes of the microbiome in an iron (Fe)-rich, plutonium-contaminated, monomictic reservoir (Pond B, Savannah River Site, South Carolina, USA). The microbial community composition varied with depth during seasonal thermal stratification and was strongly correlated with redox. During stratification, Fe(II) oxidizers (e.g., Ferrovum, Rhodoferax, Chlorobium) were most abundant in the hypoxic/anoxic zones, while Fe(III) reducers (e.g., Geothrix, Geobacter) dominated the deep, anoxic zone. Sulfate reducers and methanogens were present in the anoxic layer, likely contributing to iron and plutonium cycling. Multinomial regression of predicted functions/pathways identified metabolisms highly associated with stratification (within the top 5%), including iron reduction, methanogenesis, C1 compound utilization, fermentation, and aromatic compound degradation. Two sediment cores collected at the Inlet and Outlet of the pond were dominated by putative fermenters and organic matter (OM) degraders. Overall, microbiome analyses revealed the potential for three microbial impacts on the plutonium and iron biogeochemical cycles: (1) plutonium bioaccumulation throughout the water column, (2) Pu–Fe-OM-aggregate formation by Fe(II) oxidizers under microaerophilic/aerobic conditions, and (3) Pu–Fe-OM-aggregate or sediment reductive dissolution and organic matter degradation in the deep, anoxic waters.

54 ENVIRONMENTAL SCIENCES↗