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At least 307 records · Page 17

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DOE OSTI2025

Structures of vertebrate R2 retrotransposon complexes during target-primed reverse transcription and after second-strand nicking

R2 retrotransposons are site-specific eukaryotic non–long terminal repeat retrotransposons that copy and paste into gene loci encoding ribosomal RNAs. Recently, we demonstrated that avian A-clade R2 proteins achieve efficient and precise insertion of transgenes into their native safe-harbor loci in human cells. The features of A-clade R2 proteins that support gene insertion are not well characterized. Here, we report high-resolution cryo–electron microscopy structures of two vertebrate A-clade R2 proteins at the initiation of target-primed reverse transcription and after cDNA synthesis and second-strand nicking. Using biochemical and cellular assays, we illuminate the basis for high selectivity of template use and unique roles for each of the three zinc-finger domains in nucleic acid recognition. Reverse transcriptase active site architecture is reinforced by an unanticipated insertion motif specific to vertebrate A-clade R2 proteins. Our work provides the first insights into A-clade R2 protein structure during gene insertion and may enable future improvement and adaptation of R2-based systems for precise transgene insertion.

Science & Technology - Other Topics↗

DOE OSTI2023

A Stabilizer Framework for the Contextual Subspace Variational Quantum Eigensolver and the Noncontextual Projection Ansatz

Quantum chemistry is a promising application for noisy intermediate-scale quantum (NISQ) devices. However, quantum computers have thus far not succeeded in providing solutions to problems of real scientific significance, with algorithmic advances being necessary to fully utilize even the modest NISQ machines available today. We discuss a method of ground state energy estimation predicated on a partitioning of the molecular Hamiltonian into two parts: one that is noncontextual and can be solved classically, supplemented by a contextual component that yields quantum corrections obtained via a Variational Quantum Eigensolver (VQE) routine. This approach has been termed Contextual Subspace VQE (CS-VQE); however, there are obstacles to overcome before it can be deployed on NISQ devices. The problem we address here is that of the ansatz, a parametrized quantum state over which we optimize during VQE; it is not initially clear how a splitting of the Hamiltonian should be reflected in the CS-VQE ansätze. We propose a “noncontextual projection” approach that is illuminated by a reformulation of CS-VQE in the stabilizer formalism. This defines an ansatz restriction from the full electronic structure problem to the contextual subspace and facilitates an implementation of CS-VQE that may be deployed on NISQ devices. We validate the noncontextual projection ansatz using a quantum simulator and demonstrate chemically precise ground state energy calculations for a suite of small molecules at a significant reduction in the required qubit count and circuit depth.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

NASA NTRS1974

The dyadic diffraction coefficient for a curved edge

A compact dyadic diffraction coefficient for electromagnetic waves obliquely incident on a curved edge formed by perfectly conducting curved or plane surfaces is obtained. This diffraction coefficent remains valid in the transition regions adjacent to shadow and reflection boundaries, where the diffraction coefficients of Keller's original theory fail. The method is on Keller's method of the canonical problem, which in this case is the perfectly conducting wedge illuminated by plane, cylindrical, conical, and spherical waves. When the proper ray fixed coordinate system is introduced, the dyadic diffraction coefficient for the wedge is found to be the sum of only two dyads, and it is shown that this is also true for the dyadic diffraction coefficients of higher order edges. One dyad contains the acoustic soft diffraction coefficient; the other dyad contains the acoustic hard diffraction coefficient. The expressions for the acoustic wedge diffraction coefficients contain Fresnel integrals, which ensure that the total field is continuous at shadow and reflection boundaries. The diffraction coefficients have the same form for the different types of edge illumination; only the arguments of the Fresnel integrals are different. Since diffraction is a local phenomenon, and locally the curved edge structure is wedge shaped, this result is readily extended to the curved edge.

Kouyoumjian, R. G.↗

NASA NTRS1992

Tracking Retroreflective Targets On A Structure

Optoelectronic system simultaneously measures positions of 50 retroreflective targets with 35 degree field of view, with accuracy of 0.1 mm. SHAPES, "spatial, high-accuracy, position-encoding sensor", illuminates targets with lasers in its sensor module. System repeats measurements 10 times per second and gives unambiguous indication of distance to each target. Applicable in rendezvous and docking systems, boresight determination and precise pointing of antennas.

Nerheim, Noble M.↗

NASA NTRS1994

Oscillations of red dwarfs in evolved low-mass binaries with neutron stars

We investigate a novel aspect of a problem related to the properties of low-mass binaries (LMBs) with millisecond pulsars: the pulsations of the red dwarf (donor) companion of the neutron star (NS). The illumination of the donor star by the pulsar's high-energy nonthermal radiation and relativistic wind may substantially affect its structure. We present a quantitative analysis of the oscillation spectrum of a red dwarf which has evolved in an LMB and has undergone the stage of evaporation. We calculate the p- and g-modes for red dwarfs with masses in the interval (0.2-0.6) stellar mass. For comparison, similar calculations are presented for zero age main-sequence (ZAMS) stars of the same masses. For less massive donor stars (approximately 0.2 stellar mass) the oscillation spectrum becomes quantitatively different from that of their ZAMS counterparts. The differnce is due to the fact that a ZAMS star of 0.2 stellar mass is fully convective, while the donor star in an LMB is expected to be far from thermal equilibrium and not fully convective. As a result, in contrast to a low-mass ZAMS star, a red dwarf of the same mass in an LMB allows the existence of g-modes. We also consider tidally forced g-modes, and perform a linear analysis of these oscillations for different degrees of nonsynchronism between the orbital and spin rotation of the red dwarf component. We demonstrate the existence of a series of reasonances for the low-order g-modes which may occur in LMBs at a late stage of their evolution. We discuss the possibility that these oscillations may trigger Roche lobe overflow and sudden mass loss by the donor star. Further implications of this effect for gamma- and X-ray burst phenomena are outlined.

Sarna, Marek J.↗

NASA NTRS2010

NASA Tech Briefs, September 2010

Topics covered include: Instrument for Measuring Thermal Conductivity of Materials at Low Temperatures; Multi-Axis Accelerometer Calibration System; Pupil Alignment Measuring Technique and Alignment Reference for Instruments or Optical Systems; Autonomous System for Monitoring the Integrity of Composite Fan Housings; A Safe, Self-Calibrating, Wireless System for Measuring Volume of Any Fuel at Non-Horizontal Orientation; Adaptation of the Camera Link Interface for Flight-Instrument Applications; High-Performance CCSDS Encapsulation Service Implementation in FPGA; High-Performance CCSDS AOS Protocol Implementation in FPGA; Advanced Flip Chips in Extreme Temperature Environments; Diffuse-Illumination Systems for Growing Plants; Microwave Plasma Hydrogen Recovery System; Producing Hydrogen by Plasma Pyrolysis of Methane; Self-Deployable Membrane Structures; Reactivation of a Tin-Oxide-Containing Catalys; Functionalization of Single-Wall Carbon Nanotubes by Photo-Oxidation; Miniature Piezoelectric Macro-Mass Balance; Acoustic Liner for Turbomachinery Applications; Metering Gas Strut for Separating Rocket Stages; Large-Flow-Area Flow-Selective Liquid/Gas Separator; Counterflowing Jet Subsystem Design; Water Tank with Capillary Air/Liquid Separation; True Shear Parallel Plate Viscometer; Focusing Diffraction Grating Element with Aberration Control; Universal Millimeter-Wave Radar Front End; Mode Selection for a Single-Frequency Fiber Laser; Qualification and Selection of Flight Diode Lasers for Space Applications; Plenoptic Imager for Automated Surface Navigation; Maglev Facility for Simulating Variable Gravity; Hybrid AlGaN-SiC Avalanche Photodiode for Deep-UV Photon Detection; High-Speed Operation of Interband Cascade Lasers; 3D GeoWall Analysis System for Shuttle External Tank Foreign Object Debris Events; Charge-Spot Model for Electrostatic Forces in Simulation of Fine Particulates; Hidden Statistics Approach to Quantum Simulations; Reconstituted Three-Dimensional Interactive Imaging; Determining Atmospheric-Density Profile of Titan; Digital Microfluidics Sample Analyzer; Radiation Protection Using Carbon Nanotube Derivatives; Process to Selectively Distinguish Viable from Non-Viable Bacterial Cells; and TEAMS Model Analyzer.

Source record↗

DOE OSTI2020

Caveat mutator: alanine substitutions for conserved amino acids in RNA ligase elicit unexpected rearrangements of the active site for lysine adenylylation

Naegleria gruberi RNA ligase (NgrRnl) exemplifies the Rnl5 family of adenosine triphosphate (ATP)-dependent polynucleotide ligases that seal 3'-OH RNA strands in the context of 3'-OH/5'-PO 4 nicked duplexes. Like all classic ligases, NgrRnl forms a covalent lysyl–AMP intermediate. A two-metal mechanism of lysine adenylylation was established via a crystal structure of the NgrRnl•ATP•(Mn 2+ ) 2 Michaelis complex. Here we conducted an alanine scan of active site constituents that engage the ATP phosphates and the metal cofactors. We then determined crystal structures of ligase-defective NgrRnl-Ala mutants in complexes with ATP/Mn 2+ . The unexpected findings were that mutations K170A, E227A, K326A and R149A (none of which impacted overall enzyme structure) triggered adverse secondary changes in the active site entailing dislocations of the ATP phosphates, altered contacts to ATP, and variations in the numbers and positions of the metal ions that perverted the active sites into off-pathway states incompatible with lysine adenylylation. Each alanine mutation elicited a distinctive off-pathway distortion of the ligase active site. Our results illuminate a surprising plasticity of the ligase active site in its interactions with ATP and metals. More broadly, they underscore a valuable caveat when interpreting mutational data in the course of enzyme structure-function studies.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRSDate not supplied

Towers: Critical Initial Infrastructure for the Moon, Such as a Power Module Support

Towers are an efficient structure commonly used to elevate systems against a gravity field, such as above the surface of a planet or the Moon. Towers can be used to elevate antennas, transmitters and sensors for communication, navigation, and observation infrastructure in support of surface operations. Near the Lunar poles, elevation of rotating solar panels enables nearly continuous panel illumination for solar power production despite the Sun remaining near the horizon year-round. Further, once technology is developed for tower assembly, this same technology can be extended to the assembly of a variety of structures including rocket blast containment shields, bridges, shelters, and habitats from either Earth-sourced or Lunar-sourced structural members. Environmental factors on the Lunar surface are vastly different than those found on Earth and have significant impact on the design of towers. Towers designed for the Lunar surface have no heritage nor Earth analog. The designs discussed herein are therefore breaking new ground creating a fundamentally new class of structures outside of current experience and intuition. The purpose of this paper is to: a) describe the unique environmental factors affecting tower design on the Lunar surface compared to Earth-based systems, b) evaluate two structural forms for the tower; telescoping tubes and an assembled truss, as well as c)provide recommendations on when each structural form is most suitable. A major contribution presented in the current paper is to provide parametric plots and supporting equations of mass and volume versus module power level and blanket height above the Lunar surface for the solar array application, to enable system level studies of optimum power module distribution and size. A secondary contribution are plots of the mass and volume of aluminum or structural glass required for assembled truss towers formed from Lunar derived structural members. An objective of achieving 1 MW of total power has been used to enable comparison of structural forms, because the total mass of solar arrays is consistent. It will be shown that for moderate module sizes, up to ~50 kW power modules, based on a telescoping tube tower have advantages, but for power modules from 50 kW to 200 kW an assembled truss tower can be created with half to one third of the tube tower mass. More significantly, when shipped from Earth, an assembled truss tower can be packed into 1/14th to 1/25th of the tube tower volume. Truss designs in the 100 kW to 200 kW range will be shown to be a favorable when assembled from either graphite-epoxy angles shipped from Earth or aluminum angles fabricated from Lunar materials. 50 kW towers are an attractive choice for both a telescoping or assembled tower with the solar arrays elevated 10 m above the Lunar surface, while increased solar array elevation favors 100 kW to 200 kW power modules.

assembly↗

NASA NTRS2013

Tomographic Sounding of Protoplanetary and Transitional Disks: Using Inner Disk Variability at Near to Mid-IR Wavelengths to Probe Conditions in the Outer Disk

Spitzer synoptic monitoring of young stellar associations has demonstrated that variability among young stars and their disks is ubiquitous. The Spitzer studies have been limited by target visibility windows and cover only a short temporal baseline in years. A complementary approach is to focus on stars chosen for high-value observations (e.g. high-contrast imaging, interferometry, or access to wavelengths which are difficult to achieve from the ground) where the synoptic data can augment the imagery or interferometry as well as probing disk structure. In this talk, we discuss how synoptic data for two protoplanetary disks, MWC 480 and HD 163296, constrain the dust disk scale height, account for variable disk illumination, and can be used to locate emission features, such as the IR bands commonly associated with PAHs in the disk, as part of our SOFIA cycle 1 study. Similar variability is now known for several pre-transitional disks, where synoptic data can be used to identify inner disks which are not coplanar with the outer disk, and which may be relicts of giant planet-giant planet scattering events. Despite the logistical difficulties in arranging supporting, coordinated observations in tandem with high-value observations, such data have allowed us to place imagery in context, constrained structures in inner disks not accessible to direct imagery, and may be a tool for identifying systems where planet scattering events have occurred.

target visibility windows↗

DOE OSTI2020

Reversible inhibition and reactivation of electron transfer in photosystem I

In photosystem I (PSI) complexes at room temperature light induced electron transfer can proceed down multiple protein-cofactor branches, and the process can be an order of magnitude faster on one branch compared to the other. One factor that might contribute to this branch asymmetry is a tryptophan amino acid at position 673 on the psaB protein. This amino acid is located between two of the pigments involved in electron transfer on the psaB protein branch. The corresponding residue on the psaA protein branch is a glycine amino acid. Microsecond time-resolved step-scan FTIR difference spectroscopy at 77 K has been used to study isolated PSI complexes from wild type and a mutant where the tryptophan residue was changed to phenylalanine. Photoaccumulated FTIR difference spectra indicate changes in the protein structure upon mutation. In the mutant we also find that the electron transfer processes is inhibited following long periods of repetitive flash illumination at room temperature. This is due to double protonation of the pigment involved in electron transfer. However, we show that we can restore electron transfer functionality by incubating the light-treated mutant PSI samples in the presence of newly added (not protonated) pigment.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2023

Record quantum efficiency from strain compensated superlattice GaAs/GaAsP photocathode for spin polarized electron source

Photocathodes based on GaAs and other III–V semiconductors are capable of producing highly spin-polarized electron beams. GaAs/GaAsP superlattice photocathodes exhibit high spin polarization; however, the quantum efficiency (QE) is limited to 1% or less. To increase the QE, we fabricated a GaAs/GaAsP superlattice photocathode with a Distributed Bragg Reflector (DBR) underneath. This configuration creates a Fabry–Pérot cavity between the DBR and GaAs surface, which enhances the absorption of incident light and, consequently, the QE. These photocathode structures were grown using molecular beam epitaxy and achieved record quantum efficiencies exceeding 15% and electron spin polarization of about 75% when illuminated with near-bandgap photon energies.

47 OTHER INSTRUMENTATION↗

NASA NTRS1981

Radiometer design concepts for large aperture microwave radiometer spacecraft

Concepts involving active and passive microwave systems for soil-moisture monitoring are discussed. It is shown that the first major development efforts should be directed toward the simpler passive design concepts. Subsequently, five passive design concepts for a microwave radiometer spacecraft are outlined and compared. Some common technology needs, such as large space structures and controls, are shown to exist. Also, some peculiar technology needs are identified, such as complicated phasing networks, dielectric lenses, tapered illumination, and reflector-surface irregularity and distortion control techniques. More detailed studies to address these design concepts and assess the associated technology needs are recommended.

Keafer, L. S., Jr.↗

NASA NTRS1982

Ultraviolet images of M101 - Observations of dust and inferences on the metallicity

Since extreme Population I dominates three rocketborne telescope UV images of the Sc I galaxy NGC 5457, the delineation of the spiral structure is sharper than that of ground photographs. The UV pictures, in which the H II regions appear as reflection nebulae and illumine the nearby dust, reveal faint arms which, although correlated with the 21 cm H I radiograph, are only marginally detectable on deep visual photographs. The surface brightness of the arms is explainable in terms of UV light scattering off dust from hot stars and bright H II regions, in which case the dust would have extinction properties and a dust-to-gas ratio similar to that in the solar neighborhood and would in addition suggest that the NGC 5457 outer arm metal content is similar to that of the galaxy.

Stecher, T. P.↗

NASA NTRS1995

Validating spatial structure in canopy water content using geostatistics

Heterogeneity in ecological phenomena are scale dependent and affect the hierarchical structure of image data. AVIRIS pixels average reflectance produced by complex absorption and scattering interactions between biogeochemical composition, canopy architecture, view and illumination angles, species distributions, and plant cover as well as other factors. These scales affect validation of pixel reflectance, typically performed by relating pixel spectra to ground measurements acquired at scales of 1m(exp 2) or less (e.g., field spectra, foilage and soil samples, etc.). As image analysis becomes more sophisticated, such as those for detection of canopy chemistry, better validation becomes a critical problem. This paper presents a methodology for bridging between point measurements and pixels using geostatistics. Geostatistics have been extensively used in geological or hydrogeolocial studies but have received little application in ecological studies. The key criteria for kriging estimation is that the phenomena varies in space and that an underlying controlling process produces spatial correlation between the measured data points. Ecological variation meets this requirement because communities vary along environmental gradients like soil moisture, nutrient availability, or topography.

Sanderson, E. W.↗

NASA NTRS1986

Effects of norflurazon, an inhibitor of carotenogenesis, on abscisic acid and xanthoxin in the caps of gravistimulated maize roots

Maize seeds were germinated in the dark in the presence of the carotenoid synthesis inhibitor norflurazon and the levels of abscisic acid, xanthoxin and total carotenoids were measured in the root cap and in the adjacent 1.5 mm segment. In norflurazon-treated roots abscisic acid levels were markedly reduced, but an increase occurred in the levels of xanthoxin, a compound structurally and physiologically similar to abscisic acid. In the cultivar of maize (Zea mays L. cv. Merit) used for this work, brief illumination of the root is required for gravitropic curving. Following illumination both control and norflurazon-treated roots showed normal gravitropic curvature; however, the rate of curvature was delayed in norflurazon-treated roots. Our data from norflurazon-treated roots are consistent with a role for xanthoxin in maize root gravitropism. The increase in xanthoxin in the presence of an inhibitor of carotenoid synthesis suggests that xanthoxin and abscisic acid originate, at least in part, via different metabolic pathways.

NASA Program Space Biology↗

NASA NTRS2014

Chromospheric Variability: Analysis of 36 years of Time Series from the National Solar Observatory/Sacramento Peak Ca II K-line Monitoring Program

Analysis of more than 36 years of time series of seven parameters measured in the NSO/AFRL/Sac Peak K-line monitoring program elucidates five elucidates five components of the variation: (1) the solar cycle (period approx. 11 years), (2) quasi-periodic variations (periods approx 100 days), (3) a broad band stochastic process (wide range of periods), (4) rotational modulation, and (5) random observational errors. Correlation and power spectrum analyses elucidate periodic and aperiodic variation of the chromospheric parameters. Time-frequency analysis illuminates periodic and quasi periodic signals, details of frequency modulation due to differential rotation, and in particular elucidates the rather complex harmonic structure (1) and (2) at time scales in the range approx 0.1 - 10 years. These results using only full-disk data further suggest that similar analyses will be useful at detecting and characterizing differential rotation in stars from stellar light-curves such as those being produced by NASA's Kepler observatory. Component (3) consists of variations over a range of timescales, in the manner of a 1/f random noise process. A timedependent Wilson-Bappu effect appears to be present in the solar cycle variations (1), but not in the stochastic process (3). Component (4) characterizes differential rotation of the active regions, and (5) is of course not characteristic of solar variability, but the fact that the observational errors are quite small greatly facilitates the analysis of the other components. The recent data suggest that the current cycle is starting late and may be relatively weak. The data analyzed in this paper can be found at the National Solar Observatory web site http://nsosp.nso.edu/cak_mon/, or by file transfer protocol at ftp://ftp.nso.edu/idl/cak.parameters.

Solar physics↗

NASA NTRS2015

Simulation and Measurement of Stray Light in the CLASP

We are planning an international rocket experiment Chromospheric Lyman-Alpha Spectro-Polarimeter (CLASP) is (2015 planned) that Lyman Alpha line polarization spectroscopic observations from the sun. The purpose of this experiment, detected with high accuracy of the linear polarization of the Ly􀉲 lines to 0.1% by using a Hanle effect is to measure the magnetic field of the chromosphere-transition layer directly. For total flux of the sun visible light overwhelmingly larger and about 200 000 times the Ly􀉲 line wavelength region, also hinder to 0.1% of the polarization photometric accuracy achieved in the stray light of slight visible light. Therefore we were first carried out using the illumination design analysis software called stray light simulation CLASP Light Tools. Feature of this simulation, using optical design file (ZEMAX format) and structural design file (STEP format), to reproduce realistic CLASP as possible to calculate machine is that it was stray study. And, at the stage in the actual equipment that made the provisional set of CLASP, actually put sunlight into CLASP using coelostat of National Astronomical Observatory of Japan, was subjected to measurement of stray light (San test). Pattern was not observed in the simulation is observed in the stray light measurement results need arise that measures. However, thanks to the stray light measurement and simulation was performed by adding, it was found this pattern is due to the diffracted light at the slit. Currently, the simulation results is where you have taken steps to reference. In this presentation, we report the stray light simulation and stray light measurement results that we have implemented

CLASP↗

NASA NTRS1982

LSST (Hoop/Column) Maypole Antenna Development Program, phase 1, part 1

The first of a two-phase program was performed to develop the technology necessary to evaluate, design, manufacture, package, transport and deploy the hoop/column deployable antenna reflector by means of a ground based program. The hoop/column concept consists of a cable stiffened large diameter hoop and central column structure that supports and contours a radio frequency reflective mesh surface. Mission scenarios for communications, radiometer and radio astronomy, were studied. The data to establish technology drivers that resulted in a specification of a point design was provided. The point design is a multiple beam quadaperture offset antenna system wich provides four separate offset areas of illumination on a 100 meter diameter symmetrical parent reflector. The periphery of the reflector is a hoop having 48 segments that articulate into a small stowed volume around a center extendable column. The hoop and column are structurally connected by graphite and quartz cables. The prominence of cables in the design resulted in the development of advanced cable technology. Design verification models were built of the hoop, column, and surface stowage subassemblies. Model designs were generated for a half scale sector of the surface and a 1/6 scale of the complete deployable reflector.

Sullivan, M. R.↗