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At least 325 records · Page 18

Evaluation of DNA Vaccine Candidates against Foot-and-Mouth Disease Virus in Cattle

We evaluated four DNA vaccine candidates for their ability to produce virus-like particles (VLPs) and elicit a protective immune response against Foot-and-mouth disease virus (FMDV) in cattle. Two traditional DNA plasmids and two DNA minicircle constructs were evaluated. Both the pTarget O1P1-3C plasmid and O1P1-3C minicircle encoded a wild-type FMDV 3C protease to process the P1-2A polypeptide, whereas the O1P1-HIV-3CT minicircle used an HIV-1 ribosomal frameshift to down-regulate expression of a mutant 3C protease. A modified pTarget plasmid with a reduced backbone size, mpTarget O1P1-3C LT , used a 3C protease containing two mutations reported to enhance expression. All constructs produced mature FMDV P1 cleavage products in transfected cells, as seen by western blot analysis. Three constructs, O1P1-3C minicircles, pTarget O1P1-3C, and mpTarget O1P1-3C LT plasmids, produced intracellular VLP crystalline arrays detected by electron microscopy. Despite VLP formation in vitro, none of the DNA vaccine candidates elicited protection from clinical disease when administered independently. Administration of pTarget O1P1-3C plasmid enhanced neutralizing antibody titers when used as a priming dose prior to administration of a conditionally licensed adenovirus-vectored FMD vaccine. Further work is needed to develop these DNA plasmid-based constructs into standalone FMD vaccines in cattle.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

High thermal conductivity in wafer-scale cubic silicon carbide crystals

High thermal conductivity electronic materials are critical components for high-performance electronic and photonic devices as both active functional materials and thermal management materials. We report an isotropic high thermal conductivity exceeding 500 W m –1 K –1 at room temperature in high-quality wafer-scale cubic silicon carbide (3C-SiC) crystals, which is the second highest among large crystals (only surpassed by diamond). Furthermore, the corresponding 3C-SiC thin films are found to have record-high in-plane and cross-plane thermal conductivity, even higher than diamond thin films with equivalent thicknesses. Our results resolve a long-standing puzzle that the literature values of thermal conductivity for 3C-SiC are lower than the structurally more complex 6H-SiC. We show that the observed high thermal conductivity in this work arises from the high purity and high crystal quality of 3C-SiC crystals which avoids the exceptionally strong defect-phonon scatterings. Moreover, 3C-SiC is a SiC polytype which can be epitaxially grown on Si. We show that the measured 3C-SiC-Si thermal boundary conductance is among the highest for semiconductor interfaces. These findings provide insights for fundamental phonon transport mechanisms, and suggest that 3C-SiC is an excellent wide-bandgap semiconductor for applications of next-generation power electronics as both active components and substrates.

42 ENGINEERING↗

1-20 micron infrared photometry of 3CR radio galaxies

Seven emission-line radio galaxies in the wavelength range from 1 to 20 microns were observed in February 1983. Three broad emission-line galaxies (BLRGs 3C 109, 3C 234, and 3C 445), and four narrow emission-line radio galaxies (NLRGs 3C 98, 3C 198, 3C 223, and 3C 293) were found. The BLRGs showed strong infrared fluxes beyond 3.5 microns, with steep infrared slopes similar to optical slopes. In a comparison with earlier observational data it was found that two of the BLRGs are variable in the JHK filter range without a change of slope. The NLRGs showed strong excess at 10 microns and normal elliptical galaxy colors at JHK and L. Simple predictions based on type-2 Seyfert galaxies show that strong infrared excesses are absent.

Elvis, M.↗

Probing the Relativistic Jets of Active Galactic Nuclei with Multiwavelength Monitoring

The work completed includes the analysis of observations obtained during Cycle 7 (March 2002-February 2003) of the Rossi X-ray Timing Explorer (RXTE). The project was part of a longer-term, continuing program to study the X-ray emission process in blazars and radio galaxies in collaboration with Dr. Ian McHardy (U. of Southampton, UK) and Prof. Thomas Balonek (Colgate U.). The goals of the program are to study the X-ray emission mechanism in blazars and radio galaxies and the relation of the X-ray emission to changes in the relativistic jet. The program includes contemporaneous brightness and linear polarization monitoring at radio and optical wavelengths, total and polarized intensity imaging at at 43 GHz with a resolution of 0.1 milliarcseconds with the VLBA, and well-sampled X-ray light curves obtained from a series of approved RXTE programs. The objects studied in the time period covered by the grant were 3C 120, 3C 279, PKS 1510-089, and 3C 273, all with radio jets containing bright knots that appear to move at superluminal speeds. During RXTE Cycle 7, the project was awarded RXTE time to monitor PKS 1510-089 two times per week, 3C 273 and 3C 279 three times per week, and 3C 120 four times per week. In addition, 3C273 and 3C 279 were observed several times per day during a ten-day period in April 2002. The X-ray data, including those from earlier cycles, were compared with radio measurements obtained in the centimeter-wave band by the monitoring program of Drs. Margo and Hugh Aller at the University of Michigan Radio Astronomy Observatory, monthly imaging observations with the VLBA at 43 GHz, and optical observations obtained at several telescopes around the world.

Marscher, Alan P.↗

Physics of compact nonthermal sources. II Determination of physical parameters

A set of restrictions upon the magnetic field and visible angular size of a canonical nonthermal source (one whose emission is incoherent electron synchrotron) has been obtained in another paper. The degree of electron anisotropy and magnetic field disordering were left as free parameters. In the present paper these restrictions are applied to the variable compact radio sources 3C 84, 3C 120, 3C 273, 3C 279, 3C 454.3, CTA 102, 3C 446, PKS 2134 + 004, VRO 42.22.01, and OJ 287. The theoretically derived angular sizes for these sources are in reasonable agreement with published VLBI sizes, provided that the characteristic pitch angles are not extremely small. The magnetic field strengths determined from the degree of circular polarization are generally compatible with field strengths derived from the self-absorption and synchrotron self-Compton conditions, again provided that the pitch angles are not too small. It is argued that the self-absorption optical depth cannot be much less than unity near the low-frequency turnovers; otherwise, serious discrepancies with observation result.

Jones, T. W.↗

Physics of compact nonthermal sources. III - Energetic considerations

The energy content of the compact incoherent electron-synchrotron sources 3C 84, 3C 120, 3C 273, 3C 279, 3C 454.3, CTA 102, 3C 446, PKS 2134+004, VRO 42.22.01 and OJ 287 is calculated on the assumption that the low-frequency turnovers in the radio spectrum are due to self-absorption and that the electron distribution is isotropic. The dependence of the source parameters on various modifications of the standard assumptions is determined. These involve relativistic motions, alternate explanations for the low-frequency turnover, proton-synchrotron radiation, and distance to the source. The canonical interpretation is found to be accurate in many respects; some of the difficulties and ways of dealing with them are discussed in detail.

Burbidge, G. R.↗

A search for hard X-rays from five strong extragalactic radio sources

Data from the University of California at San Diego (UCSD) hard X-ray instrument on OSO-7 have been used to search the regions containing 3C 390.3, M87, 3C 273, 3C 111, and 3C 129. We have a weak detection of 3C 390.3 and a possible detection of 3C 111. Sensitive upper limits on hard X-rays have been obtained for the other objects. The available observational data indicate a correlation between hard X-ray emission, self-absorbed gigahertz radio emission, and strong optical emission lines in extragalactic sources. Self-Compton X-ray models have been calculated for the sources observed in this paper, and the angular sizes, lifetimes, and B fields in these sources have been estimated. These model calculations agree with existing VLBI and time variability data.

Mushotzky, R. F.↗

90-GHz flux-density measurements of variable radio sources

Results are presented for measurements of the flux densities of 10 variable extragalactic sources at 85.2 or 90 GHz, which were made over a period of almost seven years with the NRAO 36-ft millimeter-wave antenna. The primary flux-density calibration standards used include Jupiter, Saturn, Mars, and the small-diameter Galactic source DR 21. Measured flux densities are given as a function of time (in years) for the sources 3C 84, NRAO 150, 3C 120, OJ 287, 4C 39.25, 3C 273, 3C 279, 3C 345, BL Lac, and 3C 454.3. No statistically meaningful flux-density changes during an observing interval (1 to 3 days) are detected for any source, and a high degree of correlation between flux-density variations at 85.2 or 90 GHz and those observed at lower frequencies is found in all 10 sources. Some variations observed at different frequencies in several individual sources are briefly discussed.

Hobbs, R. W.↗

150 GHz observations of three radio galaxies

Radio galaxies were observed for the first time at 150 GHz. The central sources in Cyg A and 3C 111 were detected, as were the two radio lobes of Cyg A. No emission was found from the central source in 3C 236. The millimeter-wavelength emission from the central sources in Cyg A and 3C 111 is probably due in each case to a compact component that is optically thin to synchrotron radiation above about 35 GHz. These components are similar, although the compact component in 3C 111 is more luminous at both radio and X-ray wavelengths than the one in Cyg A. The 3C 111 central source was much weaker than anticipated on the basis of prior observations at 90 GHz, perhaps due to variability. Even at 150 GHz, there is no evidence of a high-frequency cutoff in the spectrum of the central source in Cyg A, and variability of this source also is expected. The flux densities of the Cyg A lobes are consistent with the power laws derived at lower frequencies, indicating that no steepening occurs in their spectra out to at least 150 GHz. There is no evidence for a compact, optically thick component in 3C 236.

Kafatos, M.↗

Variability of compact radio sources at a wavelength of 1 millimeter

Broad-band 1 mm continuum observations of 3C 273, 3C 279, BL Lac, 3C 84, OJ 287, and 3C 345 made between 1977 March and 1981 January are reported. With the possible exception of 3C 279, all of the objects exhibited evidence for variability in the 1 mm flux density on the time scale of a few months. Flux variations at wavelengths of 1 mm and 2 cm are well correlated for the 'blazars' (BL Lac objects and optically violent variable quasars) observed; emission outbursts occur simultaneously and have similar amplitude at the two wavelengths. In contrast, an outburst in the flux from the Seyfert galaxy 3C 84 was observed at a wavelength of 1 mm in early 1980 but has not appeared at 2 cm as of 1 yr later. The observed relationship between 'blazar' emission variability at 1 mm and 2 cm is inconsistent with the canonical expanding-source model of radio variability or any of its modifications. Instead, models in which the number of synchrotron-radiating electrons is time variable (due to particle injection or acceleration behind a relativistic shock) are required.

Ennis, D. J.↗

X-ray View of Four High-Luminosity Swift-BAT AGN: Unveiling Obscuration and Reflection with Suzaku

Aims. A complete census of obscured Active Galactic Nuclei (AGN) is necessary to reveal the history of the super massive black hole (SMBH) growth and galaxy evolution in the Universe given the complex feedback processes and the fact that much of this growth occurs in an obscured phase. In this context, hard X-ray surveys and dedicated follow-up observations represent a unique tool for selecting highly absorbed AGN and for characterizing the obscuring matter surrounding the SMBH. Here we focus on the absorption and reflection occurring in highly luminous, quasar-like AGN, to study the relation between the geometry of the absorbing matter and the AGN nature (e.g. X-ray, optical, and radio properties), and to help to determine the column density dependency on the AGN luminosity. Methods. The Swift/BAT nine-month survey observed 153 AGN, all with ultra-hard X-ray BAT fluxes in excess of 10(exp −11) erg per square centimeter and an average redshift of 0.03. Among them, four of the most luminous BAT AGN (44.73 less than LogLBAT less than 45.31) were selected as targets of Suzaku follow-up observations: J2246.0+3941 (3C 452), J0407.4+0339 (3C 105), J0318.7+6828, and J0918.5+0425. The column density, scattered/reflected emission, the properties of the Fe K line, and a possible variability are fully analyzed. For the latter, the spectral properties from Chandra, XMM-Newton and Swift/XRT public observations were compared with the present Suzaku analysis, adding an original spectral analysis when non was available from the literature. Results. Of our sample, 3C 452 is the only certain Compton-thick AGN candidate because of i) the high absorption (N(sub H) approximately 4 × 10(exp 23) per square centimeter) and strong Compton reflection; ii) the lack of variability; iii) the "buried" nature, i.e. the low scattering fraction (less than 0.5%) and the extremely low relative [OIII] luminosity. In contrast 3C 105 is not reflection-dominated, despite the comparable column density, X-ray luminosity and radio morphology, but shows a strong long-term variability in flux and scattering fraction, consistent with the soft emission being scattered from a distant region (e.g., the narrow emission line region). The sample presents high (greater than 100) X-to- [OIII] luminosity ratios, with an extreme value of R(sup X)(sub [OIII]) approximately 800 for 3C 452, confirming the [OIII] luminosity to be affected by residual extinction in presence of mild absorption, especially for "buried" AGN such as 3C 452. Three of our targets are powerful FRII radio galaxies, which is shown by their high luminosity and absorption; this makes them the most luminous and absorbed AGN of the BAT Seyfert survey despite the inversely proportional N(sub H) - L(sub X) relation.

SwiftBAG AGN↗

Photoaging of phenolic secondary organic aerosol in the aqueous phase: evolution of chemical and optical properties and effects of oxidants

Abstract. While gas-phase reactions are well established to have significant impacts on the mass concentration, chemical composition, and optical properties of secondary organic aerosol (SOA), the aqueous-phase aging of SOA remains poorly understood. In this study, we performed a series of long-duration photochemical aging experiments to investigate the evolution of the composition and light absorption of the aqueous SOA (aqSOA) from guaiacyl acetone (GA), a semivolatile phenolic carbonyl that is common in biomass burning smoke. The aqSOA was produced from reactions of GA with hydroxyl radical (•OH-aqSOA) or a triplet excited state of organic carbon (3C∗-aqSOA) and was then photoaged in water under conditions that simulate sunlight exposure in northern California for up to 48 h. The effects of increasing aqueous-phase •OH or 3C∗ concentration on the photoaging of the aqSOA were also studied. High-resolution aerosol mass spectrometry (HR-AMS) and UV–Vis spectroscopy were utilized to characterize the composition and the light absorptivity of the aqSOA and to track their changes during aging. Compared to •OH-aqSOA, the 3C∗-aqSOA is produced more rapidly and shows less oxidation, a greater abundance of oligomers, and higher light absorption. Prolonged photoaging promotes fragmentation and the formation of more volatile and less light-absorbing products. More than half of the initial aqSOA mass is lost, and substantial photobleaching occurs after 10.5 h of prolonged aging under simulated sunlight illumination for 3C∗-aqSOA and 48 h for •OH-aqSOA. By performing positive matrix factorization (PMF) analysis of the combined HR-AMS and UV–Vis spectral data, we resolved three generations of aqSOA with distinctly different chemical and optical properties. The first-generation aqSOA shows significant oligomer formation and enhanced light absorption at 340–400 nm. The second-generation aqSOA is enriched in functionalized GA species and has the highest mass absorption coefficients in 300–500 nm, while the third-generation aqSOA contains more fragmented products and is the least light absorbing. These results suggest that intermediately aged phenolic aqSOA is more light absorbing than other generations, and that the light absorptivity of phenolic aqSOA results from a competition between brown carbon (BrC) formation and photobleaching, which is dependent on aging time. Although photoaging generally increases the oxidation of aqSOA, a slightly decreased O/C of the •OH-aqSOA is observed after 48 h of prolonged photoaging with additional •OH exposure. This is likely due to greater fragmentation and evaporation of highly oxidized compounds. Increased oxidant concentration accelerates the transformation of aqSOA and promotes the decay of BrC chromophores, leading to faster mass reduction and photobleaching. In addition, compared with •OH, photoaging by 3C∗ produces more low-volatility functionalized products, which counterbalances part of the aqSOA mass loss due to fragmentation and evaporation.

54 ENVIRONMENTAL SCIENCES↗

1 millimeter continuum observations of extragalactic objects

Continuum observations of 23 extragalactic objects have been made at a wavelength of 1 mm; nine of the 23 have been definitely detected at this wavelength. The sources detected include seven from which the 1-mm emission appears to be nonthermal, and two from which the emission appears to be thermal radiation from dust. Repeated observations of the brightest nonthermal sources permitted a search for 1-mm variations on time scales of one month to three years. BL Lac was observed to vary by more than a factor of 2 in flux, and 3C 84, 3C 120, and 3C 273 showed possible variations; 3C 279 showed no variations greater than + or - 30%. The 1-mm variations of BL Lac appear to be correlated with variations at radio wavelengths. The other sources with nonthermal spectra detected were 3C 111 and M87; the two thermal sources detected were NGC 253 and M82. Comparison of the latter two 1-mm measurements with 2.6-mm CO measurements suggests that the CO line in these galaxies is not heavily saturated.

Elias, J. H.↗

An interlaminar tension strength specimen

This paper describes a technique to determine interlaminar tension strength, sigma(sub 3c) of a fiber reinforced composite material using a curved beam. The specimen was a unidirectional curved beam, bent 90 degrees, with straight arms. Attached to each arm was a hinged loading mechanism which was held by the grips of a tensile testing machine. Geometry effects of the specimen, including the effects of loading arm length, inner radius, thickness, and width, were studied. The data sets fell into two categories: low strength corresponding to a macroscopic flaw related failure and high strength corresponding to a microscopic flaw related failure. From the data available, the loading arm length had no effect on sigma(sub 3c). The inner radius was not expected to have a significant effect on sigma(sub 3c), but this conclusion could not be confirmed because of differences in laminate quality for each curve geometry. The thicker specimens had the lowest value of sigma(sub 3c) because of poor laminate quality. Width was found to affect the value of sigma(sub 3c) only slightly. The wider specimens generally had a slightly lower strength since more material was under high stress, and hence, had a larger probability of containing a significant flaw.

Jackson, Wade C.↗