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Results for “Activity detection”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 325 records · Page 18
All sourcesNASA NTRSDOE OSTI
NASA NTRS1992

Wall to membrane linkers, stretch activated channels, and the detection of tension, voltage, temperature, auxin, and pH

Introduction. The higher plant is a heterogeneous, mechanically prestressed structure continually subject to shifting forces. When a cell grows in a plant at gravitropic equilibrium, it must create localized maxima of shear in walls of neighboring cells. Such mechanical stress and strain are likely detected in a variety of ways. However, tension-sensitive ion channels are of particular interest because it appears that they are elaborately evolved for sensory function. We hypothesize that 1) the patchy patterns of high shear are focused via wall-to-membrane linkers onto the plasma membrane, where 2) they are translated by mechanosensory cation channels into corresponding patterns of high cytosolic Ca2+, which 3) initiate local enhancement of wall expansion. Further, we hypothesize that the local promotion of enhancement is achieved at least in part by local intensification of auxin transport across the plasma membrane. By implication, when an organ is asymmetrically pressed, rubbed, or bent or when it is displaced in the gravitational field, the net asymmetry of shear stress occurring across the organ would lead to asymmetric redistribution of auxin and corrective asymmetric growth. We shall describe a representative mechanosensitive Ca(2+) -selective cation channel (MCaC) with susceptibilities to xenobiotics implicating it as a force transducer in thigmo- and gravitropism. Then, we shall consider whether a putative wall-to-membrane linker (WML) could be a key feature of the molecular architecture permitting the stress distributed in the wall system to be focused on the channels.

NASA Discipline Number 40-50↗
DOE OSTI2012

3,3′-Diindolylmethane, but not indole-3-carbinol, inhibits histone deacetylase activity in prostate cancer cells

Increased consumption of cruciferous vegetables is associated with a reduced risk of developing prostate cancer. Indole-3-carbinol (I3C) and 3,3′-diindolylmethane (DIM) are phytochemicals derived from cruciferous vegetables that have shown promise in inhibiting prostate cancer in experimental models. Histone deacetylase (HDAC) inhibition is an emerging target for cancer prevention and therapy. We sought to examine the effects of I3C and DIM on HDACs in human prostate cancer cell lines: androgen insensitive PC-3 cells and androgen sensitive LNCaP cells. I3C modestly inhibited HDAC activity in LNCaP cells by 25% but no inhibition of HDAC activity was detected in PC-3 cells. In contrast, DIM significantly inhibited HDAC activity in both cell lines by as much as 66%. Decreases in HDAC activity correlated with increased expression of p21, a known target of HDAC inhibitors. DIM treatment caused a significant decrease in the expression of HDAC2 protein in both cancer cell lines but no significant change in the protein levels of HDAC1, HDAC3, HDAC4, HDAC6 or HDAC8 was detected. Taken together, these results show that inhibition of HDAC activity by DIM may contribute to the phytochemicals' anti-proliferative effects in the prostate. The ability of DIM to target aberrant epigenetic patterns, in addition to its effects on detoxification of carcinogens, may make it an effective chemopreventive agent by targeting multiple stages of prostate carcinogenesis. -- Highlights: ► DIM inhibits HDAC activity and decreases HDAC2 expression in prostate cancer cells. ► DIM is significantly more effective than I3C at inhibiting HDAC activity. ► I3C has no effect on HDAC protein expression. ► Inhibition of HDAC activity by DIM is associated with increased p21 expression. ► HDAC inhibition may be a novel epigenetic mechanism for cancer prevention with DIM.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2025

Performance Evaluation of a Novel Sequence-Based Directional Detection Strategy for Protection of Active Distribution Networks

Directional elements are relied on to achieve selectivity in fault detection in power systems. Although such elements have been deployed successfully for many years, there is an increased need for novel methods to deal with the unique challenges of directional protection in modern distribution networks. This article analyzes the impact of inverter-based resources (IBRs) on existing directional protection methods in distribution systems. It identifies parts of such elements that pose a risk of misoperation when IBRs are used in distribution networks. The authors have developed a new directional detection method for unbalanced faults in such networks using superimposed symmetrical sequence quantities. The phase angle of the superimposed negative sequence admittance is used to determine fault direction. The paper also presents a real-time co-simulation platform between a simulated distribution system and physical protection relay, using OPAL-RT. An SEL-411L relay is used to program the detection algorithm. This hardware-in-the-loop (HIL) setup is used to verify the performance of the method and the results are compared with existing directional methods

24 POWER TRANSMISSION AND DISTRIBUTION↗
DOE OSTI2023

CRISPR/Cas activity assays and compositions thereof

The present invention relates, in part, to methods for detecting nuclease activity, such as the activity of Cas nucleases. Also described herein are compositions for conducting assays, as well as methods for conducting assays in the presence of test compounds.

Seamon, Kyle Jeffrey↗
NASA NTRS1988

Impact of device level faults in a digital avionic processor

This paper describes an experimental analysis of the impact of gate and device-level faults in the processor of a flight control system. Via mixed mode simulation faults were injected both at the gate (stuck-at) and at the transistor levels, and their propagation through the chip to the output pins was measured. The results show that there is little correspondence between a stuck-at and a device-level fault model insofar as error activity or detection within a functional unit is concerned. Insofar as error activity outside the injected unit and at the output pins are concerned, the stuck-at and device models track each other, although the stuck-at model overestimates, by over one hundred percent, the probability of fault propagation to the output pins. The stuck-at model significantly underestimates the impact of an internal chip fault on the output pins.

Kim, S.↗
NASA NTRS1978

IPS activity observed as a precursor of solar induced terrestrial activity

A radio telescope designed to exploit the interplanetary scintillation (IPS) technique and locate, map, and track solar wind disturbances which result in geomagnetic disturbances, thereby providing a forecast capability, is described. Preliminary results from operation of the telescope include: (1) evidence for a precursor signal in the IPS activity with a 1-2 day lead time with respect to density enhancements which frequently give rise to geomagnetic activity; (2) detection of a spectral broadening signature which also serves as a precursor of geomagnetic activity; (3) out-of-the-ecliptic plasma density enhancements which were not detected by near-Earth, ecliptic plane spacecraft; (4) detection of 12 corotating density enhancements;(5) detection of over 80 sources which give detectable scintillation of which 45 have been used for detailed synoptic analysis and 9 for spectral analysis; and (6) measurement of 0-lag coefficient of 0.56 between density and IPS activity enhancements.

Cronyn, W. M.↗
NASA NTRS2023

Wildfire-fighting Use Case Requirements to Monitor

In this technical report, we provide requirements for a wildfire-fighting use-case, towards the Safety Demonstrator 1. The use case will incorporate ground and airborne assets operating in a coordinated fashion, and will comprise five activities, from detection to the execution of the initial attack. Depending on the activity and the data involved, the requirements identified may be non-probabilistic or probabilistic. In both cases, we first identify some of the requirements we wish to monitor, and then present a formalization using the language of requirements of the NASA requirements elicitation tool FRET. To formalize probabilistic requirements, we use a novel extension to FRET’s requirements language that incorporates notions of probability, and discuss how requirements can be translated into existing probabilistic temporal logics like PCTL. We exemplify how some of the requirements presented can be monitored using the existing tools Ogma and Copilot. We close with a summary and future directions.

Requirements↗
NASA NTRS1988

Ultraviolet and radio flares from UX Arietis and HR 1099

Simultaneous observations of the RS CVn systems UX Ari and HR 1099 with the IUE satellite and the VLA are presented. Flaring activity is observed at ultraviolet wavelengths with the IUE when none is detected at radio wavelengths with the VLA. Radio flares with no detectable ultraviolet activity have also been observed. Thus, flares in the two spectral regions are either uncorrelated or weakly correlated. The flaring emission probably originates in different regions at the two wavelengths. Radio flares from RS CVn stars may originate in sources that are larger than, or comparable to, a star in size. This is in sharp contrast to compact, coherent radio flares from dwarf M stars. The ultraviolet flares from RS CVn stars probably originate in sources that are smaller than a component star.

Lang, Kenneth R.↗
NASA NTRS1989

Impact of device level faults in a digital avionic processor

This study describes an experimental analysis of the impact of gate and device-level faults in the processor of a Bendix BDX-930 flight control system. Via mixed mode simulation, faults were injected at the gate (stuck-at) and at the transistor levels and, their propagation through the chip to the output pins was measured. The results show that there is little correspondence between a stuck-at and a device-level fault model, as far as error activity or detection within a functional unit is concerned. In so far as error activity outside the injected unit and at the output pins are concerned, the stuck-at and device models track each other. The stuck-at model, however, overestimates, by over 100 percent, the probability of fault propagation to the output pins. An evaluation of the Mean Error Durations and the Mean Time Between Errors at the output pins shows that the stuck-at model significantly underestimates (by 62 percent) the impact of an internal chip fault on the output pins. Finally, the study also quantifies the impact of device fault by location, both internally and at the output pins.

Suk, Ho Kim↗
NASA NTRS1985

Reduced phenylalanine ammonia-lyase and tyrosine ammonia-lyase activities and lignin synthesis in wheat grown under low pressure sodium lamps

Wheat (Triticum aestivum L. cv Fremont) grown in hydroponic culture under 24-hour continuous irradiation at 560 to 580 micromoles per square meter per second from either metalhalide (MH), high pressure sodium (HPS), or low pressure sodium (LPS) lamps reached maturity in 70 days. Grain yields were similar under all three lamps, although LPS-grown plants lodged at maturity. Phenylalanine ammonia-lyase (PAL) and a tyrosine ammonia lyase (TAL) with lesser activity were detected in all extracts of leaf, inflorescence, and stem. Ammonia-lyase activities increased with age of the plant, and plants grown under the LPS lamp displayed PAL and TAL activities lower than wheat cultured under MH and HPS radiation. Greenhouse solar-grown wheat had the highest PAL and TAL activities. Lignin content of LPS-grown wheat was also significantly reduced from that of plants grown under MH or HPS lamps or in the greenhouse, showing a correlation with the reduced PAL and TAL activities. Ratios of far red-absorbing phytochrome to total phytochrome were similar for all three lamps, but the data do not yet warrant a conclusion about specific wavelengths missing from the LPS lamps that might have induced PAL and TAL activities in plants under the other lamps.

Triticum/enzymology/growth & development/metabolis↗
DOE OSTI2019

A High‐Throughput Mass Spectrometric Enzyme Activity Assay Enabling the Discovery of Cytochrome P450 Biocatalysts

Abstract Assaying for enzymatic activity is a persistent bottleneck in biocatalyst and drug development. Existing high‐throughput assays for enzyme activity tend to be applicable only to a narrow range of biochemical transformations, whereas universal enzyme characterization methods usually require chromatography to determine substrate turnover, greatly diminishing throughput. We present an enzyme activity assay that allows the high‐throughput mass‐spectrometric detection of enzyme activity in complex matrices without the need for a chromatographic step. This technology, which we call probing enzymes with click‐assisted NIMS (PECAN), can detect the activity of medically and biocatalytically significant cytochrome P450s in cell lysate, microsomes, and bacteria. Using this approach, a cytochrome P450 BM3 mutant library was successfully screened for the ability to catalyze the oxidation of the sesquiterpene valencene.

de Rond, Tristan↗
DOE OSTI2025

Remote-sensing detectability of airborne Arctic dust

Remote-sensing (RS)-based estimates of Arctic dust are oftentimes overestimated due to a failure in separating out the dust contribution from that of spatially homogeneous clouds or low-altitude cloud-like plumes. A variety of illustrations are given with a particular emphasis on questionable claims of using brightness temperature differences (BTDs) as a signature indicator of Arctic dust transported from mid-latitude deserts or generated by local Arctic sources. While there is little dispute about the presence of both Asian and local dust across the Arctic, the direct RS detectability of airborne dust, as ascribed to satellite (MODIS and AVHRR) measurements of significantly negative brightness temperature differences at 11 and 12 µm (BTD 11–12 ), has been misrepresented in certain cases. While it is difficult to account for all examples of strongly negative BTD 11–12 values in the Arctic, it is unlikely that airborne dust plays a significant role. One much more likely contributor would be water clouds in the Arctic inversion layer. The RS detectability of the impact of Arctic dust (notably due to Arctic dust from local sources) can, however, be of significance. Sustained dust deposition can substantially decrease (visible to shortwave IR) snow and ice reflectance albedo (pan-chromatic reflectance) and the signal measured by satellite sensors. Significantly negative BTD 11–12 values would, however, only represent a limited area near the drainage basin sources according to our event-level case studies. The enhanced ice-nucleating particle (INP) role of local Arctic dust can, for example, induce significant changes in the properties of low-level mixed-phase clouds (cloud optical depth changes <~ 1) that can readily be detected by active and passive RS instruments. It is critical that the distinction between the RS detectability of airborne Arctic dust versus the RS detectability of the impacts of that dust be understood if we are to appropriately parameterize, for example, the radiative forcing influence of dust in this climate-sensitive region.

54 ENVIRONMENTAL SCIENCES↗