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Cytoplasmic Amplification of Transcriptional Noise Generates Substantial Cell-to-Cell Variability

Transcription is an episodic process characterized by probabilistic bursts, but how the transcriptional noise from these bursts is modulated by cellular physiology remains unclear. Using simulations and single-molecule RNA counting, we examined how cellular processes influence cell-to-cell variability (noise). The results show that RNA noise is higher in the cytoplasm than the nucleus in ~85% of genes across diverse promoters, genomic loci, and cell types (human and mouse). Measurements also show further amplification of RNA noise in the cytoplasm, fitting a model of biphasic mRNA conversion between translation- and degradation-competent states. This multi-state translation-degradation of mRNA also causes substantial noise amplification in protein levels, ultimately accounting for ~74% of intrinsic protein variability in cell populations. Overall, the results demonstrate how noise from transcriptional bursts is intrinsically amplified by mRNA processing, leading to a large super-Poissonian variability in protein levels.

59 BASIC BIOLOGICAL SCIENCES↗

Axenisation of oleaginous microalgal cultures via anoxic photosensitisation

Growing interest in sustainable food and biofuel research has necessitated high quality axenic oleaginous microalgal strains. Unfortunately, most strains available in culture banks contain commensal microbes such as bacteria and the default decontamination method involves antibiotic treatment which has begun to exacerbate the emergence of antibiotic resistance. To overcome this problem, anoxic photosensitisation was investigated as an alternate approach. Four oleaginous microalgal species (Tetradesmus obliquus, Desmodesmus armatus, Chlorella vulgaris and Nannochloropsis limnetica) were incubated in varying concentrations of Rose Bengal (0 μM, 1 μM, 3 μM or 9 μM) either in normal (oxic) or anoxic conditions, for 72 h under light (8.85 ± 0.4 W/m 2 ) in a specially designed heterotrophic growth complex (HGC) medium, followed by 72 h in standard Bold's Basal Medium (BBM). Commonly used antibiotics-based protocol was used as the control method. Post treatment, cell numbers and percentage populations were counted with Flow Cytometry, and viability was tested using standard plating methods using BBM and LB. Additionally, the contaminating microbes in the cultures were profiled using 16Ss rRNA sequencing. Anoxic conditions were able to significantly decrease bacterial content, albeit with an equally detrimental effect on the microalgal population. Although the responses differed between the microalgae, anoxic incubation along with Rose Bengal at 3 μM was able to completely decontaminate N. limnetica and C. vulgaris, while D. armatus and T. obliquus could be decontaminated with an additional streak-plating step. None of the cultures could be decontaminated using antibiotics treatment. These results suggest that axenisation of microalgal cultures was largely due to anoxy, that was synergistically enhanced by Rose Bengal at a concentration of ≥3 μM.

59 BASIC BIOLOGICAL SCIENCES↗

CASPI: collaborative photon processing for active single-photon imaging

Image sensors capable of capturing individual photons have made tremendous progress in recent years. However, this technology faces a major limitation. Because they capture scene information at the individual photon level, the raw data is sparse and noisy. Here we propose CASPI: Collaborative Photon Processing for Active Single-Photon Imaging, a technology-agnostic, application-agnostic, and training-free photon processing pipeline for emerging high-resolution single-photon cameras. By collaboratively exploiting both local and non-local correlations in the spatio-temporal photon data cubes, CASPI estimates scene properties reliably even under very challenging lighting conditions. We demonstrate the versatility of CASPI with two applications: LiDAR imaging over a wide range of photon flux levels, from a sub-photon to high ambient regimes, and live-cell autofluorescence FLIM in low photon count regimes. We envision CASPI as a basic building block of general-purpose photon processing units that will be implemented on-chip in future single-photon cameras.

47 OTHER INSTRUMENTATION↗

Model run output for WM-ABM 2024 HESS Publication

Model output for computational experiments introducing WM-ABM. See associated HESS publication here: https://egusphere.copernicus.org/preprints/2023/egusphere-2023-1604/ all_nldas_cdl_data_v3.txt - Cropland Data Layer data aggregated to 1/8 degree resolution for input into the PMP calibration process. Variables include NLDAS ID, crop type, year, the count of pixels in the grid cell of the crop type, CDL crop ID, and GCAM crop ID. 20230115 ABM runs - WM-ABM model outputs for various scenarios described in HESS publication. If multiple versions exist (e.g., v2, v3, etc.) latest version indicates dataset generated for manuscript. The "baseline" run is the primary run, where as the "memXX" runs indicate sensitivity runs testing different parameters of agent memory. Output files starting with "abm_results_" include groundwater irrigated acres and surface water irrigated acres for each farm/agent combination. Other CSV outputs provide summary statistics of various agent and water shortage outcomes, used for generation of figures in the HESS manuscript.

ABM↗

Microspheres

Vital information on a person's physical condition can be obtained by identifying and counting the population of T-cells and B-cells, lymphocytes of the same shape and size that help the immune system protect the body from the invasion of disease. The late Dr. Alan Rembaum developed a method for identifying the cells. The method involved tagging the T-cells and B-cells with microspheres of different fluorescent color. Microspheres, which have fluorescent dye embedded in them, are chemically treated so that they can link with antibodies. With the help of a complex antibody/antigen reaction, the microspheres bind themselves to specific 'targets,' in this case the T-cells or B-cells. Each group of cells can then be analyzed by a photoelectronic instrument at different wavelengths emitted by the fluorescent dyes. Same concept was applied to the separation of cancer cells from normal cells. Microspheres were also used to conduct many other research projects. Under a patent license Magsphere, Inc. is producing a wide spectrum of microspheres on a large scale and selling them worldwide for various applications.

Source record↗

Genetic models in applied physiology: selected contribution: effects of spaceflight on immunity in the C57BL/6 mouse. I. Immune population distributions

There are several aspects of the spaceflight environment that may lead to changes in immunity: mission-related psychological stress, radiation, and changes in gravity. On December 5, 2001, the space shuttle Endeavor launched for a 12-day mission to examine these effects on C57BL/6 mice for the first time. On their return, assays were performed on the spleen, blood, and bone marrow. In response to flight, there were no significant differences in the general circulating leukocyte proportions. In contrast, there was an increase in splenic lymphocyte percentages, with a corresponding decrease in granulocytes. There was an overall shift in splenic lymphocytes away from T cells toward B cells, and a decrease in the CD4-to-CD8 ratios due to a decrease in T helpers. In contrast, there were proportional increases in bone marrow T cells, with decreases in B cells. Although the blast percentage and count were decreased in flight mice, the CD34(+) population was increased. The data were more consistent with a shift in bone marrow populations rather than a response to changes in the periphery. Many of the results are similar to those using other models. Clearly, spaceflight can influence immune parameters ranging from hematopoiesis to mature leukocyte mechanisms.

Non-NASA Center↗

Effects of 2-deoxy-D-glucose administration on immune parameters in mice

Physical exercise and diet alterations have been shown to affect immune parameters. Similar effects are also induced by the administration of the non-metabolizable glucose analog, 2-deoxy-D-glucose (2-DG). The current study was designed to characterize the effects of glucoprivation induced by 2-DG administration on leukocyte subset distribution and function. BDF1 mice (n = 8 per group) were injected intraperitoneally one or three times with 0, 500, 750, 1000 or 1500 mg/kg of 2-DG. Two hours after the last injection of 2-DG, immunological parameters were analyzed. A dose-dependent increase in plasma glucose concentrations of mice injected once with up to 1500 mg/kg of 2-DG was observed (p < 0.001). After either one or three injections of up to 1500 mg/kg of 2-DG, corticosterone levels, leukocyte counts in the spleen, and CD3+ cells in the thymus increased. In vitro proliferation of partially purified lymphocytes from the spleen in the presence of both concanavalin-A and lipopolysaccharide decreased in a dose dependent manner (p < 0.05). In addition, after three injections, the proportion of both thymocytes and splenocytes bearing alphabeta-TCR increased as the concentration of 2-DG increased (p < 0.01). These results demonstrate that 2-DG administration induced dose-dependent changes in both thymus and spleen cell distribution and function.

NASA Discipline Regulatory Physiology↗

Demand Capacity Balancing at Vertiports for Initial Strategic Conflict Management of Urban Air Mobility Operations

Urban Air Mobility (UAM) is a new transportation concept that enables highly automated, cooperative, passenger or cargo-carrying air transportation services in and around urban areas. To achieve the high level of operational density and complexity desired by the UAM community, an airspace system that allows UAM operators to readily access and operate safely and efficiently in the airspace is needed. This airspace system will require air traffic management designed to reduce the risk of conflicts and loss of separation between UAM flights. In general, strategic conflict management is considered as the first layer of conflict management for safe flight operations to condition the traffic to reduce the need for airborne separation provision, the second layer of conflict management. Demand Capacity Balancing (DCB) is one of the concept components to achieve strategic conflict management. DCB strategically evaluates traffic demand and resource capacities to allow UAM operators to determine when, where and how they operate, while mitigating conflicting needs for airspace and vertiport capacity. DCB can be applied whenever UAM demand exceeds the capacity in airspace or at vertiports. As the UAM ecosystem evolves with advanced technologies and matured operational procedures, more complicated conflict management will likely be needed. In the current UAM ‘Concept of Operation (ConOps) 1.0’ operational stage defined by FAA, however, it will be meaningful to explore the demand capacity balancing at vertiports only, as an initial strategic conflict management approach for UAM operations because vertiport capacity seems to be a bottleneck of UAM traffic. For this research, we developed a demand-capacity imbalance detection and resolution service for UAM. This DCB service identifies the demand from operators and compares the demand to a given capacity at the shared resources (i.e., vertiports) over the upcoming time horizon which is divided into time bins having a constant interval. When a new flight plan is submitted, the algorithm embedded in the DCB service checks the available time bins based on the desired departure time and estimated arrival time at origin and destination vertiports, respectively. If the time bins for the originally desired times are already occupied by other flights (i.e., demand is at or above capacity), the algorithm finds the next available time bins for takeoff and landing and shifts the conflicting departure time to the earliest time that satisfies the capacity constraints at both origin and destination vertiports. The details of the algorithm will be described in the final manuscript. Figure 1 shows that the proposed DCB algorithm works well for a sample traffic scenario. In this example, a total of 144 flights, split between two operators, are planned over 2 hours, traveling 10 routes between five vertiports. In the heatmaps, the horizontal axis shows 12 time bins where each bin represents a 12-minute interval, and the vertical axis shows five vertiports. The number in each cell shows the number of operations, counting both departures and arrivals, at a specific vertiport in each time bin. For the given capacity of 2 operations/vertiport/bin, Figure 1 shows that the original demand sometimes exceeds the capacity, but the modified demand is reduced to the given capacity after resolving demand-capacity imbalances. When UAM flights are operated, it is expected that many practical issues would arise in the federated system architecture with multiple operators. UAM operators may experience a time synchronization issue due to communication delay between operator and vehicle. UAM vehicles would fly at different flight speeds, depending on vehicle models. Actual departure and arrival times can have large variations, compared to the schedule. The lead time from flight plan submission to desired departure time can vary by service type (e.g., regular shuttle service vs. on-demand service). Using the proposed DCB algorithm, we also investigated how the actual flight schedule and DCB performance are affected by these uncertainties such as unsynchronized times between operators, flight speed differences, lead time differences, and departure time errors. The final manuscript will include the background of this research work, the description of the DCB algorithm and its use cases with traffic scenarios. It will also provide the analytical results about the impact of various uncertainties that can occur in actual UAM operations on the DCB at vertiports, in terms of demand distribution changes, number of simultaneous operations, and delay propagation.

Urban Air Mobility↗

Implicit Large-Eddy Simulations of Compressible Mixing Layers

Implicit large-eddy simulations of the self-similar regions of two compressible mixing layers at high Reynolds number and convective Mach numbers of 0.381 and 0.690 were carried out. Experimental data was used as the input into a synthetic eddy method turbulent inflow boundary condition to initiate simulations. Computational grids with nearly isotropic cells were used and the grid counts ranged from 24 to 270 million points. Agreement with experimental velocity and Reynolds stress data was good given the simplifications of the simulation. The decrease in mixing layer growth rate with increasing compressibility compares well with the literature. Budgets for the Reynolds stress transport equation were extracted from the simulations. It was demonstrated that the grids used did not resolve the dissipation term at the experimental Reynolds number. However, low Reynolds number simulations indicate that the numerical dissipation replaces the physical dissipation and the dissipation term in the transport equation can be represented by the summation term that quantifies the imbalance in the budget. The budgets for the high Reynolds number mixing layers agree well with previous low Reynolds number studies; indicating that the primary mechanism for the reduced growth rate and increased anistropy of the Reynolds stress tensor due to compressibility is a reduction in the pressure-strain and production terms. A new scaling based on the magnitude of the Reynolds stress tensor is proposed. This scaling provides better relative comparisons between the data and shows that the decrease in pressure-strain in the shear component is the primary driver in reducing the transverse normal stress.

turbulence↗

Comparative toxicity assessment of novel Si quantum dots and their traditional Cd-based counterparts using bacteria models Shewanella oneidensis and Bacillus subtilis

Quantum dots are crystalline semiconductor nanoparticles with unique optical properties due to quantum confinement effects. They have several advantages compared to traditional organic fluorescent dyes, such as high fluorescent brightness, photostability, and tunable emission wavelengths, dependent upon particle size. Their unique optical properties have led to an increased use in a variety of devices, including diode lasers and television displays, as well as in biomedical research. The most commonly used quantum dots (QDs) are made of cadmium selenide (CdSe) and have cadmium selenide core with zinc sulfide shell (CdSe/ZnS), containing inherently toxic cadmium. This work focuses on comparison of the toxic effects of conventional CdSe and CdSe/ZnS QDs and silicon QDs, which are emerging as a potentially benign alternative, using bacteria as model organisms. The bacteria models used for our studies are Shewanella oneidensis MR-1, a Gram-negative bacterium, and Bacillus subtilis SB 491, a Gram-positive bacterium. Furthermore, this research assesses changes in cell viability, respiration pattern, and cell membrane integrity in the presence of the nanoparticles using colony counting, respirometry and membrane integrity assays, respectively. The association of the QDs with bacterial cell membranes was investigated using transmission electron microscopy (TEM). Results indicate that the silicon QDs are benign to the bacteria considered, and they do not associate with the cell membranes. The CdSe cores exhibit significant toxicity to the bacterial cells, whereas the CdSe/ZnS QDs are comparatively less toxic.

54 ENVIRONMENTAL SCIENCES↗

Regulation of hematopoiesis in rats exposed to antiorthostatic, hypokinetic/hypodynamia. I - Model description

The effect of a 7-day suspension in a jacket and harness with 20-deg head-down tilt on body weight, food and water consumption, and hematological parameters is investigated experimentally in male Sprague-Dawley rats weighing 150-175 g. The results are presented in graphs and compared with those for unsuspended controls and with published data on rats and humans exposed to microgravity in space flight. Suspended rats are found to have reduced red-blood-cell mass, erythropoiesis, plasma volume (leading to temporarily increased hematocrit), body weight, and food and water consumption; rightward-shifted oxyhemoglobin-dissociation curves; and unchanged platelet count, leucocyte count or PHA reactivity, and red-blood-cell shape distribution. Since many of these effects are also seen in space flight, the present experimental model is considered a promising technique for simulating the hematopoietic effects of microgravity at 1 g.

Dunn, C. D. R.↗

Survival of B. Horneckiae Spores Under Ground-simulated Space Conditions

To prevent forward contamination and maintain the scientific integrity of future life detection missions, it is important to characterize and attempt to eliminate terrestrial microorganisms associated with exploratory spacecraft and landing vehicles. Among the organisms isolated from spacecraft-associated habitats, spore-forming microbes are highly resistant to various physical and chemical conditions, which include ionizing and UV radiation, desiccation and oxidative stress, and the harsh environment of outer space or planetary surfaces. Recently a radiation resistant, spore forming bacterial isolate, Bacillus horneckiae, was isolated from a clean room of the Kennedy Space Center where the Phoenix spacecraft was assembled. The exceptionally high tolerance of extreme conditions demonstrated by sporeforming bacteria highlighted the need to assess the viability of these microbes in situ (in real) space. The proposed BOSS (Biofilm Organisms Surfing Space) project aims to understand the mechanisms by which biofilm forming organisms, such as B. horneckiae, will potentially be able to withstand harsh space conditions. As previously stated, the spore producing ability of these species gives them increased survivability to harsh conditions. Some of the spores will have the protective exosporium layer artificially removed before the test to determine if the existence of this layer significantly changes the survivability during the mission. In preparation for that experiment, we analyzed spores which were exposed during a ground simulation, the EXPOSE R2 Biofilm Organisms Surfing Space (BOSS). Previous to exposure, spores were deposited onto spacecraft grade aluminum coupons in a spore suspension calculated to contain between 10(exp 7) and 10(exp 8) spores. This precursor series will be used to establish a baseline survivability function for comparison with the future flight tests during EXPOSE-R. For each coupon, a 10% polyvinyl alcohol (PVA) film was applied and peeled from the coupon to recover the spores. One hundred μl of sterile 10% PVA was applied to the surface of the coupon and allowed to dry for 1 hour at 37 C. The films were then removed using sterile scalpel and forceps and placed into a glass test tube containing 2 milliliters of sterile deionized water. The PVA film process was then repeated on each coupon one additional time to ensure recovery of the majority of spores. The second PVA film was added in the same glass tube as in the previous round. If the spores remained 100% viable, the test tubes should now contain between 5 X 10(exp 6) and 5 X 10(exp 7) spores per millimeter; however, it is expected that some loss of viability has occurred. In order to assess this loss, the number of colony forming, viable spores was counted. To count the colony forming units (CFUs), the spore containing solution was diluted in a process of 10-fold serial dilution by mixing successive solutions in a 100 microliter spore suspension to 900 microliter deionized H2O ratio. A sample dilution series revealed that 10(exp -3) and 10(exp -4) concentrations would be necessary for an accurate CFU count to be taken. For those two concentrations, a spread on a TSA plate was prepared and incubated at 32 C. For the samples exposed to UV radiation, the cell survivability was too low to establish a count from 100 microliter spread plating. Instead, no dilutions were performed and the entire 2 milliliter spore suspension was plated and incubated at 32 C. The plate's CFU counts were taken at 24 hours and 48 hours from the time of plating. At the end of the CFU counting the total surviving spores in each sample were calculated based on the number of CFUs that were observed per 100 microliters, or per 2 milliliters for the UV irradiated samples. The results of these calculations are shown in Figures 1 and 2.

cleanroom↗

Are Capacity and Energy Loss Equivalent Metrics for Battery Aging Reporting?

Battery aging in research publications and manufacturer specification sheets for individual cells is commonly reported as capacity (Ah) versus cycle number. However, the key measured quantity in battery-powered devices is energy (Wh), which is derived from integrating capacity with voltage. In this work, we compare the rate of capacity and energy loss across a wide range of Li-ion single-cell cycling studies with different positive electrode chemistries, charge–discharge rates, and temperatures. We find that the relative rate of discharge energy loss varies with cycling conditions. For many cells cycled under moderate conditions, the rate of discharge energy fade is only slightly faster than the rate of discharge capacity fade. However, some cells demonstrated up to a 15% decline in cycle count when 80% energy retention rather than 80% capacity retention was used as the end-of-life metric. These results highlight the importance of reporting cell aging based on energy fade to avoid overestimating battery lifetime in full systems.

batteries↗

Prevention of Spaceflight-Induced Bone Loss: A Promising Dietary Countermeasure

Space radiation is one of the challenges for long-term spaceflight, especially for missions beyond low Earth Orbit. We have shown that a diet composed of 25% dried plum (DP) prevents radiation-induced bone loss. The DP diet fully protected the cancellous bone microarchitecture of mice exposed to ionizing radiation (gamma, proton, and HZE). In particular relevant to space radiation, we showed that the DP diet prevents bone loss due to 1Gy of sequential exposure of proton (1H, low linear energy transfer -LET-), and 1Gy of iron (56Fe, high-LET). In addition, total body exposure to 1Gy of HZE radiation (56Fe) impaired the osteoprogenitors in mice fed the control diet, as indicated by decreased osteoblast mineralization. In contrast, marrow stem cells from mice fed the DP did not exhibit these deficits. Based on these promising results supporting DP as a countermeasure to prevent space radiation induced-tissue damage, we conducted additional studies to combine radiation and simulated microgravity. We exposed skeletally mature male mice to simulated microgravity (using hindlimb unloading, HU) or total body irradiation (TBI, 2Gy 137Cs) or in combination (HU+TBI). We observed bone loss in the mice fed the control diet (CD) exposed to simulated spaceflight, as measured by cancellous bone microarchitecture parameters such as percent bone volume (BV/TV). In contrast, mice fed the DP diet did not exhibit similar bone loss with either treatments (HU or TBI) or combined (HU+TBI) as seen in most parameters. This was observed in both long bones (tibia) and axial bones (vertebrae). Furthermore, preliminary data shows that pre-feeding with the DP diet attenuates the HU-induced decrement in bone-forming osteoblasts colony counts and mineralization capacity of the osteoprogenitor cells. We also performed DP feeding at lower doses (5%, 10%) and found that these doses are less effective in preventing the radiation-induced bone loss. All our studies with the DP diet as a countermeasure were done with a period of pre-feeding ranging from 14 to 21 days before irradiation, and in order to test the capacity of the DP diet as a countermeasure provided after exposure to radiation, we exposed mice to 2Gy gamma radiation and then provided the DP diet 24 hours post-IR. Preliminary data indicates that DP mitigated the radiation-induced deficits in certain cancellous bone structural parameters. Finally, we showed that mice fed with the control diet (CD) increased oxidative damage in the serum after radiation exposure, whereas mice fed the DP diet do did not show such an increase. In summary, the DP diet is a promising countermeasure for spaceflight-induced tissue damage.

Schreurs, Ann-Sofie↗

Development and Evaluation of a Cloud-Gap-Filled MODIS Daily Snow-Cover Product

The utility of the Moderate Resolution Imaging Spectroradiometer (MODIS) snow-cover products is limited by cloud cover which causes gaps in the daily snow-cover map products. We describe a cloud-gap-filled (CGF) daily snowcover map using a simple algorithm to track cloud persistence, to account for the uncertainty created by the age of the snow observation. Developed from the 0.050 resolution climate-modeling grid daily snow-cover product, MOD10C1, each grid cell of the CGF map provides a cloud-persistence count (CPC) that tells whether the current or a prior day was used to make the snow decision. Percentage of grid cells "observable" is shown to increase dramatically when prior days are considered. The effectiveness of the CGF product is evaluated by conducting a suite of data assimilation experiments using the community Noah land surface model in the NASA Land Information System (LIS) framework. The Noah model forecasts of snow conditions, such as snow-water equivalent (SWE), are updated based on the observations of snow cover which are obtained either from the MOD1 OC1 standard product or the new CGF product. The assimilation integrations using the CGF maps provide domain averaged bias improvement of -11 %, whereas such improvement using the standard MOD1 OC1 maps is -3%. These improvements suggest that the Noah model underestimates SWE and snow depth fields, and that the assimilation integrations contribute to correcting this systematic error. We conclude that the gap-filling strategy is an effective approach for increasing cloud-free observations of snow cover.

Hall, Dorothy K.↗

Toxicity and Radioprotective Effects of DF-1 and Carbon Nanotubes in Human Lung and Liver Cell Lines

The DF-1 compound, a sixty carbon fullerene derivative, has been shown to have antioxidant effects and is thought to possibly help mediate the effects of radiation on cells. While this is potentially useful, it is important to first understand the effect that the DF-1 has on the cells and the growth rate of the cells to determine if the material itself has any innate toxicity. A growth curve was established for both HF-19 cells, human fibroblasts, and HepG2 cells, liver tissue cells in the presence of two different concentrations of DF-1 and for untreated controls. The cells were plated in triplicate in 60mm dishes and were lifted and counted with a hemocytometer daily for one week. The growth curve data for the HF-19 cells show that while the low concentration of DF-1 had no apparent effect on the growth rate, the high concentration of DF-1 appeared to severely inhibit the growth of the HF-19 cells. The growth curve data for the HepG2 cells shows that the DF-1 compound had no significant effect on the rate at which the cells grew. A second growth curve study was performed plain carbon nanotubes, but with only 24 hour exposure to a high and low concentration of material. The carbon nanotubes are another carbon compound similar to DF-1, but in the shape of a tube, rather than a ball. We hypothesize that nanotubes may also mediate the effect of radiation on cells. This time, nanotubes did not showed any significant effect on the growth rate HF-19 or HepG2 cells. A third growth curve study is underway to further determine the effect of DF-1, nanotubes, and a derivatized nanotube (BHT-nanotubes). This derivatized nanotube has been modified with a compound that is known to be very effective at neutralizing free radicals. We expect that the high concentration of DF-1 and possibly the nanotubes and BHT-nanotubes may inhibit the growth of the HF-19 cells while the low concentration will resemble the growth of the control. We also hypothesize that there will be no significant effect on the growth of the HepG2 cells by the nanotubes, and BHT-nanotubes. In order to examine the usefulness of the DF-1, nanotubes, and BHT-nanotubes in mediating the effects of radiation a clonogenic assay is being performed. The HF-19 cells were plated in different concentrations of the various compounds and exposed to varying amounts of radiation. The cells are being allowed to grow in a small enough concentration so that the ability of each cell to divide can be seen by the development of cell clusters. By comparing the irradiated control to the un-irradiated control the effects of radiation alone can be seen. By comparing the compound treated irradiated cells to the irradiated control the usefulness of each compound can be seen. It is thought that Amifostine, the positive control, will have more regularly dividing cells then the irradiated control, as will DF-1 and hopefully both nanotube materials as well.

Burgoyne, Madeline↗

Characterization of Particulate Matter Profiling and Alveolar Deposition from Biomass Burning in Northern Thailand: The 7-SEAS Study

Biomass burning (BB) frequently occurs in SouthEast Asia (SEA), which significantly affects the air quality and could consequently lead to adverse health effects. The aim of this study was to characterize particulate matter (PM) and black carbon (BC) emitted from BB source regions in SEA and their potential of deposition in the alveolar region of human lungs. A 31-day characterization of PM profiling was conducted at the Doi Ang Khang (DAK) meteorology station in northern Thailand in March 2013. Substantial numbers of PM (10147 +/- 5800 # per cubic centimeter) with a geometric mean diameter (GMD) of 114.4 +/- 9.2 nm were found at the study site. The PM of less than 2.5 micron in aerodynamic diameter (PM sub 2.5) hourly-average mass concentration was 78.0 +/- 34.5 per cubic microgram whereas the black carbon (BC) mass concentration was 4.4 +/- 2.6 micrograms per cubic meter. Notably, high concentrations of nanoparticle surface area (100.5 +/- 54.6 square micrometers per cubic centimeter) emitted from biomass burning can be inhaled into the human alveolar region. Significant correlations with fire counts within different ranges around DAK were found for particle number, the surface area concentration of alveolar deposition, and BC. In conclusion, biomass burning is an important PM source in SEA, particularly nanoparticles, which has high potency to be inhaled into the lung environment and interact with alveolar cells, leading to adverse respiratory effects. The fire counts within 100 to 150 km shows the highest Pearson's r for particle number and surface area concentration. It suggests 12 to 24 hr could be a fair time scale for initial aging process of BB aerosols. Importantly, the people lives in this region could have higher risk for PM exposure.

biomass burning↗

Assessing the Impacts of Two Averaging Methods on AIRS Level 3 Monthly Products and Multi-Year Monthly Means

The Atmospheric Infrared Sounder (AIRS) onboard NASA’s Aqua satellite provides more than 16 years of data. Its monthly gridded (Level 3) product has been widely used for climate research and applications. Since counts of successful soundings in a grid cell are used to derive monthly averages, this “Averaged By Observations (ABO)” approach effectively gives equal importance to all participating soundings within a month. It is conceivable, then, that days with more observations due to day-to-day orbit shift and regimes with better retrieval skills, will contribute disproportionately to the monthly average within a cell. Alternatively, the AIRS Level 3 monthly product can be produced through an "Averaged By Days (ABD)” approach, where the monthly mean in a grid cell is a simple average of the daily means. The effects of these averaging methods on the AIRS version 6 monthly product are assessed quantitatively using temperature and water vapor at surface and 500hPa. The ABO method results in a warmer (slightly colder) global mean temperature at surface (500hPa) and a drier global mean water vapor than ABD method. The AIRS multi-year monthly mean temperature and water vapor from both methods are also compared with the Modern-Era Retrospective analysis for Research and Applications – 2 (MERRA-2) product and evaluated with a simulation experiment, indicating the ABD method has less error and is more closely correlated with MERRA-2. In summary, the ABD method is recommended for future versions of the AIRS Level 3 monthly product and more data services supporting Level 3 aggregation are needed.

AIRS↗