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At least 325 records · Page 18

Belowground respiration, root traits, and soil characteristics of an East Tennessee deciduous forest, 2019-2020

This dataset contains empirical physiological, morphological, and chemical data of root systems, and elemental, nutrient content for soils collected on forty individuals of eight temperate tree species, between June 2019 and July 2020 at The University of Tennessee Forest Research Center and Arboretum in Oak Ridge, Tennessee. The project used a novel methodology to empirically derive estimates of the autotrophic and heterotrophic components of soil respiration in-situ. The project consists of two measurement approaches. The first set of measurements uses a standard approach for measuring specific root respiration on excised root systems. The second used “in-situ root trays” This dataset includes 10 data files in comma separate (*.csv) ASCII format. Data include measurements of leaf and root functional traits for excised root systems and for living root systems housed within in-situ root trays, data on soil carbon and nitrogen pools, in-situ measurements of soil moisture and temperature, data on soil respiration rates for in-situ root trays (both as soil mass-based fluxes, and soil-area based fluxes), and data on the geographic coordinates and tree sizes of study trees. Forty study trees of eight temperate tree species were studied (five individuals per species). Two in-situ root trays were installed per species, each housing one entire root system comprising < 3 root orders, and still being attached to the tree via transportive root. All respiration measurements were conducted with the Li-6800 portable photosynthesis system (Li-COR, Lincoln, NE, USA). Root respiration measurements of excised root tissues were made using the Li-6800 and the Walz 3010-GWK1 gas exchange chamber (Heinz Walz GmbH, Effeltrich, Germany). Root scan images were analyzed using WinRHIZO. These images are companion files to this dataset and are contained in two compressed (*.zip) folders. Additional metadata are provided: 10 data dictionaries and a file-level metadata file in comma separate (*.csv) format and a user guide in PDF (*.pdf) format.

EARTH SCIENCE > BIOSPHERE > VEGETATION > LEAF CHAR↗

TARP Identifying Critical Thresholds for Acute Response of Plants and Ecosystems to Water Stress at Walker Branch Watershed, 2002-2005

This dataset contains data from a manipulative field study aimed at identifying critical thresholds for acute response of plants and ecosystems to water stress (TARP) that took place at Walker Branch Watershed in Oak Ridge, Tennessee from 2002-2005 (2002-06-20 to 2005-12-16). The study used understory tents for the removal of 100% of the growing-season throughfall and stem flow, to provide data on the impact of acute drought on mechanisms responsible for growth and mortality of deciduous forest canopy trees representative of common plant functional types (Liriodendron and Quercus). Through three years of manipulation (2003, 2004 and 2005; pretreatment measurements in 2002) various measures of tree response to surface moisture deficits were recorded including root and leaf traits, plant nonstructural carbohydrates status, hourly sapflow, basal area, and periodic observations of foliar photosynthesis and conductance. Additionally, environmental data such as air and soil temperature, soil water content, and soil matric potential were recorded. This dataset contains data 16 files in comma-separate (*.csv) format. Additional metadata are provided: 16 data dictionaries and a file-level metadata file in comma separate (*.csv) format and a user guide in PDF (*.pdf) format.

basal area↗

Leafweb: Dataset in Support of Coupled Modeling of Photophysics, Photochemistry, and Biochemistry of Photosynthesis, December 2022 Release

This data set contains measurements of leaf gas exchange and Pulse-Amplitude Modulated (PAM) fluorometry of light, carbon dioxide (CO2), oxygen (O2), and temperature responses from 26 C3 photosynthetic and four C4 photosynthetic species measured by independent researchers in Canada, China, Finland, Netherlands, and United States in the field, garden, or greenhouse. Data were collected between 1987 and 2021, however data for individual species only provide coverage over a few hours to one year. Species include three lianas, three shrubs, two boreal deciduous trees, two boreal evergreen needle-leaf tree, three temperate deciduous trees, four tropical deciduous trees, three tropical evergreen trees, one C3 grass, three C4 grasses, and six crop varieties. These measurements are conducted according to standard protocols in gas exchange (Long and Bernacchi 2003) and PAM fluorometry (Baker 2008). The Scots pine (Pinus sylvestris) dataset contains one-year continuous fluorometry observations made at intervals of 10 or 30 minutes in the field under natural environments using Walz monitoring PAM. Measurements from all other 29 species include simultaneous PAM fluorometry and gas exchange observations. Among these 29 species, seven species were measured with light response curves only (i.e., light intensity varied systematically with ambient CO2 concentration controlled at a constant level, e.g., 400 ppm). All the other 22 species were measured with both the light response and CO2 response (i.e., ambient CO2 concentration varied systematically with light intensity controlled at a constant level, e.g., 1200 µmolm-2s-1). For most species, measurements were made with temperature controlled at ~ 25 degrees Celsius (C) with the exceptions of Scots pine (natural diurnal and seasonal variations), tomato cultivar Basket Vee (~ 21 degrees C), and cotton which contained temperature stress experiments (9 to 40 degrees C). All measurements were made at ambient O2 concentration except for the tomato cultivar Basket Vee and cotton which used two O2 levels (2 and 21%) and rice and tomato cultivar Growdena which used five O2 levels (2, 10, 21, 35, and 50%). This dataset supports the complete modeling of photosynthesis at the leaf scale, including photophysical, photochemical, and biochemical reactions. It will support research in the applications of remotely sensed solar induced chlorophyll fluorescence and the responses of energy dissipation pathways to environmental variations across species and climates. This dataset contains 260 data files in comma separate (.csv) format contained inside 11 compressed (*.zip) files. Additional metadata are provided: one data dictionary, a species information table, and a file-level metadata file in comma separate (.csv) format and a user guide in PDF (*.pdf) format.

air temperature↗

Leaf-area index of Oak-Hickory Forest at Missouri Ozark (MOFLUX) site: 2007–2022

This data set contains measurements of leaf-area index (LAI) at the Missouri Ozark (MOFLUX) site during growing seasons from 2007-2022. The MOFLUX site is located in the University of Missouri Baskett Forest, a second growth oak-hickory forest situated in the Ozark Border Region of central Missouri, USA. MOFLUX is part of the AmeriFlux network (site ID: US-MOz) and an eddy covariance tower marks the middle of the site (Figure 1). During 2003, 24 circular vegetation plots (each 0.08 hectares) were established within a ~250 meter (m) radius around the tower. The plots were situated 50 m apart along 5 linear transects radiating out from the flux tower base in southeast, south, southwest, west, and northwest directions. There were 5 plots per transect except for the northwest one, which had only 4 due to the presence of a small pond at the terminus. At weekly intervals during the growing season, leaf-area index measurements were taken within each vegetation plot on the 5 transects running from the central flux tower. A plant canopy analyzer (model LAI-2000 Li-Cor Inc., Lincoln NE) was used to make the LAI measurements. We collected samples to enable measurement of the mean LAI of each transect. LAI observations are useful for understanding vegetation phenology, and to aid in the interpretation of ecosystem gas exchange. This dataset contains one data file in comma separate (.csv) format. Additional metadata are provided: one data dictionary and a file-level metadata file in comma separate (.csv) format and a user guide in PDF (*.pdf) format.

ESS-DIVE CSV File Formatting Guidelines Reporting ↗

Differential Organic Carbon Mineralization Responses to Soil Moisture in Three Different Soil Orders Under Mixed Forested System: Supporting Data

This data contains data from 90-day long incubation study which aimed to look at the soil moisture-texture relationship on soil organic carbon (SOC) cycling. Soils were collected from three distinct soil textures from mixed forests in 2017: sandy (Georgia, 2017-05-01), loamy (Missouri, 2017-06-14) and clayey (Texas, December 2017) were incubated at different soil moisture levels (air-dried, 25% water holding capacity (WHC), 50% WHC, 100% WHC and 175% WHC) at room temperature for a period of 90 days. Files contain microbial respiration, active and slow SOC pools, and their respective mineralization rates, extractable organic carbon (C), and C-acquiring extracellular enzymes. Findings from these data were used in Singh et al. (2021). This study aimed to examine the interactive effect of soil moisture and texture on SOC mineralization. Soil samples of three distinct textures (sandy, loamy, and clayey) were collected from mixed forests of Georgia, Missouri, and Texas, respectively. Soil cores of 5 cm diameter were collected from numerous random locations at each site from 0-15 cm depth after scraping the litter layer and mixed thoroughly to obtain a composite sample per site. Three additional soil cores were collected to determine the WHC using pressure plate extractors. Soil samples were composited, and triplicate soil samples were incubated in mason jars for a period of 90 days at room temperature under different moisture regimes: air dried, 25% WHC, 50% WHC, at WHC and 100% saturation. Soil respiration was measured weekly, and destructive sampling was conducted at 1, 15, 60, and 90 days to determine extractable organic C, C acquiring enzyme activity, and active and slow SOC pools with their respective mineralization rates. The C acquiring enzyme activity was the total activity of α-glucosidase, β-glucosidase, cellobiohydrolase, and β-xylosidase enzymes. Gas samples for microbial respiration measurements were collected from headspace of incubation jars through the sampling ports on the lids and then analyzed using a Shimadzu Gas Chromatograph (GC-2014). Prior to sampling, the vials were evacuated. Blank correction was also done by collecting gas samples from empty incubation jars. Double pool exponential decay model was used in SigmaPlot to determine the active and slow SOC pools and their mineralization rates (Farrar et al., 2012; Jagadamma et al., 2014). The C-acquiring extracellular enzymes were measured using the microplate method by German et al., (2011). Microbial community structure was determined using the phospholipid fatty acid (PLFA) and neutral lipid fatty acid (NLFA) analyses (Buyer and Sasser, 2012). This dataset has seven data files provided in comma-separate (*.csv) format. Additional metadata are provided: seven data dictionaries and a file-level metadata file in comma separate (*.csv) format and a user guide in PDF (*.pdf) format.

Carbon acquiring enzyme activity↗

Responses of Drying-rewetting (Transient Soil Moisture) and Steady State Soil Moisture Incubation on Soil Organic Carbon Dynamics in Three US Soils, 2017

This data set contains measurements of soil characteristics (aggregate size distribution and mean size, total aggregate associated carbon, extractable organic C, and microbial biomass C), microbial respiration, and soil metabolite concentrations from a transient and steady soil moisture incubation experiment using soils of different textures (sandy, loamy, and clayey). The study investigated mechanisms driving the Birch effect (increased carbon mineralization pulses with wetting following a drying period) in differing soil textures. Three different soils of distinctly different textures were collected from 0-15cm depth in Georgia (sandy, 2017-05-01), Missouri (loamy, 2017-06-14), and Texas (clayey, December 2017). Soils were incubated for 140 days with destructive harvests done on days 1, 29, 33, 56, 112, 116, and 140 in transient soil moisture incubation and on days 1, 33, 116, and 140 in steady state soil moisture incubation. This dataset contains six data files in comma separate (.csv) format. Additional metadata are provided: six data dictionaries and a file-level metadata file in comma separate (*.csv) format and a user guide in PDF (*.pdf) format.

1-Methyladenosine concentration↗

Volatile Organic Compounds and Meteorological Conditions in the Missouri Ozark AmeriFlux (MOFLUX) Site, 2023

This data set contains measurements of atmospheric components and meteorological conditions of central Missouri, United States during the summer of 2023. Data serve to examine the impact of meteorological conditions relevant to future climate on the emission and transformation of volatile organic compounds (VOCs). During the field campaign, researchers were also able to incorporate opportunistic analyses of the long-range transport of smoke plumes generated from extreme forest fire activities in Canada. VOC measurements were conducted at the Missouri Ozark AmeriFlux (MOFLUX) site (latitude 38.7441, longitude −92.2000) using a proton transfer reaction time of flight mass spectrometer (PTR-ToF-MS 6000 X2). The sampling campaign was conducted during the summer of 2023 (2023-06-25 to 2023-08-12) with measurements being taken at high temporal resolution (1 hour). Particular VOCs analyzed include: Methanol, Acetonitrile, Acetone, Isoprene, Methylvinyl ketone (MVK) and methacrolein (MACr), Benzene, Toluene, Catechol, and Monoterpene. Meteorological parameters included in this dataset were collected from a nearby Columbia Regional Airport (~10 km). Global solar radiation data were measured at a weather site in Ashland, MO, 5.22 km from the MOFLUX tower. The data were accessed using the MesoWest online website (https://mesowest.utah.edu/) provided by the Department of Atmospheric Sciences, University of Utah. Discussion of the data providers, database, and dissemination were highlighted in prior studies (Horel et al., 2002a; Horel et al., 2002b) . Smoke mixing ratios (in mg m−3) were estimated from the High-Resolution Rapid Refresh (HRRR) 3 km weather model for Missouri at 6-hour intervals (Dowell et al., 2022). This dataset contains three data files in comma separate (*.csv) format. Additional metadata are provided: three data dictionaries and a file-level metadata file in comma separate (*.csv) format and a user guide in PDF (*.pdf) format.

Acetone↗

Quarterly Soil Core and Root Analyses from the Missouri Ozarks AmeriFlux (MOFLUX) Site, Ashland, Missouri, 2017-2023

This dataset contains quarterly soil core measurements from the Missouri Ozarks AmeriFlux (MOFLUX) site located at the University of Missouri’s Thomas H. Baskett Wildlife Research and Education Area near Ashland, Missouri. These data will be used to parameterize an ensemble of MOFLUX-optimized soil carbon-nitrogen models, used to simulate carbon (C) and nitrogen (N) cycling responses to future hydroclimatic scenarios and the trajectory of soil C stocks with concomitant forest decline. Beginning in 2017, eight soil cores were collected approximately quarterly near plot 1 of the southeast transect, near the automated soil respiration flux chambers, from 0–15 cm depth. Data are currently available through 2023 (2017-06-14 to 2023-11-13); additional observations will be appended to this dataset as they become available. Cores were analyzed for gravimetric moisture content, pH, total carbon and nitrogen, texture, microbial biomass carbon and nitrogen, and extractable dissolved organic carbon and nitrogen. This dataset contains one data file in comma separate (*.csv) format. Additional metadata are provided: one data dictionary and a file-level metadata file in comma separate (*.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES↗

SPRUCE Advanced Molecular Techniques Provide a Rigorous Method for Characterizing Organic Matter Quality in Complex Systems: Supporting Data

This dataset contains Fourier transform ion cyclotron resonance mass spectrometry-based measurements (FTICRMS), gas chromatography, and liquid chromatography data from the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental site within the Marcell Experimental Forest in northern Minnesota, USA from 2011 to 2016. Measurement results demonstrate that optical characterization of peatland dissolved organic matter (DOM) may not fully capture classically identified chemical characteristics and may, therefore, not be the best measure of organic matter quality. Analyses show poor regression fits between optical characteristics and Fourier transform ion cyclotron resonance mass spectrometry-based measurements (FTICRMS). These data were used in Wilson and Tfaily (2018) and files are organized by which figure they were used to produce. Data underlying those regression analyses, as shown in Figures 1a and 1b of Wilson and Tfaily (2018), are provided in data files ds01 and ds02, respectively. ds01: Optically-derived specific ultraviolet absorbance method at 254 nm (SUVA254) values and Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS) -derived double bond equivalences. ds02: Optically-derived spectral slopes and FTICRMS-derived average molecular weights. Results of analyses of a representative sample by FTICRMS and additional analytical techniques provide a broader picture of the compounds occurring in peatland dissolved organic matter (DOM) as shown in Figure 2 of Wilson and Tfaily (2018). The data underlying Figure 2 in are provided in data files ds03-ds07, organized by the respective analytical technique used to generate the data. ds03: Nitrogen containing compounds identified via FTICRMS. ds04: Compounds identified via FTICRMS which did not have nitrogen. ds05: Nuclear magnetic resonance (NMR) results. ds06: Gas chromatography mass spectrometry (GCMS) results. ds07: Liquid chromatography mass spectrometry (LCMS) results. This dataset contains seven data files in comma-separate values (*.csv) format. Additional metadata are provided: seven data dictionaries and a file-level metadata file in comma-separate values (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES↗

SPRUCE Bog Surface Elevation Assessments with SPRUCE Elevation Transect Instrument, Marcell Experimental Forest, Minnesota, 2013-2025

This data set reports plot-specific bog surface elevation measurements collected with the SPRUCE Elevation Transect (SET) instrument at the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental site within the Marcell Experimental Forest in northern Minnesota, USA. The SET instrument is a modified version of the Cahoon et al. (2002) surface elevation table instrument. The device is mounted to fixed stands that are anchored in deep sediments, making it capable of identifying subtle changes in bog surface elevation over time. Measurements are reported as absolute elevation in meters above mean sea level (amsl) for two locations in each of the SPRUCE experimental treatment plots and additional ambient boardwalk plots: 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 19, 20 and 21. Measurements were collected from June 2013 through September 2025 (2013-06-02 to 2025-09-08). Changes in bog surface elevation may indicate alterations in the carbon and water cycles due to changing environmental conditions. This data set contains one data file in comma separate (*.csv) format. Additional metadata are provided: one data dictionary and a file-level metadata file in comma separate (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES↗

SPRUCE Photosynthesis and Respiration of Picea mariana and Larix laricina in SPRUCE Experimental Plots, 2016-2017

This dataset contains empirical physiological, morphological, and chemical data collected on two dominant conifer species, Picea mariana and Larix laricina, between May 2016 and August 2017 at the SPRUCE (Spruce and Peatland Responses Under Changing Environments) experimental site within the Marcell Experimental Forest in northern Minnesota, USA. Analyses assessed the response of conifers to whole-ecosystem warming and elevated atmospheric carbon dioxide (CO2) concentrations. Measurements include light-saturated net photosynthesis and dark respiration at ambient atmospheric carbon dioxide (CO2) concentration (2016), CO2 response curves of light-saturated net photosynthesis (2017), leaf morphology (leaf mass per unit leaf area) and nitrogen content (on both mass and area basis). Leaf photosynthesis and respiration were measured with portable photosynthesis systems (LI6400XT, LI6800, LI-COR Biosciences, USA). In 2016, photosynthesis and respiration were measured at a common temperature of 25 ˚C to assess thermal acclimation, and at growth temperatures (for each of the five treatment temperatures) to assess field performance. In 2017, CO2 response curves of net photosynthesis (so-called A-Ci curves) were conducted at different leaf temperatures (15, 25, 32.5, 40 and 45 ˚C) for assessment of biochemical parameters and their temperature sensitivity. In addition, dark respiration measurements at different leaf temperatures (10, 15, 20, 25, 30, 35, 40, and 45 ºC) were also taken. This dataset contains one data file in comma-separate values (*.csv) and Microsoft Excel (*.xlsx) format. Additional metadata are provided: a data dictionary and a file-level metadata file in comma-separate values (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES↗

SPRUCE NDVI Data from Selected SPRUCE Experimental Plots, 2016-2018

This dataset consists of observations of Normalized Difference Vegetation Index (NDVI) observations from the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental site within the Marcell Experimental Forest in northern Minnesota, USA. Measurements were collected via stationary sensors positioned 1.5 meter above unobstructed areas of the ground layer. Sensors were installed above undisturbed community composition plots, that are not instrumented or destructively sampled in any way, in six of the ten chambered SPRUCE experimental study plots located in the S1-Bog. Spectral data (630 and 800 nanometers) were collected at 10-minute intervals over a field of view of ~53 cm in diameter beginning May 6, 2016. Data collection ended at a different time for each sensor as batteries discharged – ranging from January 1, 2018 to April 1, 2018. The spectral sensors were manufactured by Decagon Inc., (now METER) for observation of ecosystem canopies. Sensors were deployed at the beginning of the 2016 growing season in +0, +4.5, and +9.0 C temperature treatments, with one each in an ambient and elevated carbon dioxide (CO2) plot, for a total of six sensors. These data have been analyzed and reported on in McPartland et al (2018). This dataset contains one data file in comma-separate values (*.csv) format and PDF (*.pdf) file containing plot maps. Additional metadata are provided: a data dictionary and a file-level metadata file in comma-separate values (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES↗

SPRUCE Vegetation Community Survey Data from SPRUCE Experimental Plots, 2014-2020

This dataset provides vegetation survey data collected across vegetation community plots within the 10 chambered treatment plots (6, 19, 11, 20, 4, 13, 8, 16, 10, 17) and 3 unchambered ambient plots (7, 14, 21) from the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental site within the Marcell Experimental Forest in northern Minnesota, USA. Collections occurred from 2014-2020 (2014-04-27 to 2020-08-06). The vegetation community plots, three in each experimental plot, were established in 2014 and designated to be undisturbed and reserved for long-term sampling. Vegetation survey data were collected at the peak of each growing season by placing a 1x2 meter rectangular grid over each community plot. The 1x2 meter grid is subset into 50 20x20 cm grid cells. Observations are of the presence of each species in each grid cell for each subplot. That’s a total of 39 subplots across the 13 experimental plots. From 2018 to 2020, shrub stem density was counted and percent shrub cover was estimated in five selected grid cells in each vegetation community plot. Also included are photographs of each community plot taken at the time of data collection for each measurement year. These data provide insight into the responses of plant community composition to warming and increased carbon dioxide. These data were use in analyses in McPartland et al (2018; 2020). This dataset contains three data files in comma-separate values (*.csv) format and seven compressed (*.zip) files containing 877 JPEG (*.jpg) files. Additional metadata are provided: a data dictionary and a file-level metadata file in comma-separate values (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES↗

SPRUCE Fungal Necromass Litter Bag Decomposition Study in SPRUCE Experimental Plots, 2016-2018

This dataset provided the results of a 2-year fungal necromass litter bag decomposition study at the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental site within the Marcell Experimental Forest in northern Minnesota, USA. Necromass from four mycorrhizal fungal species that naturally occur and associate with either the ectomycorrhizal (EM) tree hosts or ericoid mycorrhizal (ERM) shrubs in the S1 Bog site and vary in melanin content (Cenococcum geophilum Fr., Suillus grisellus, Meliniomyces bicolor, and Oideodendron griseum) were incubated in situ in SPRUCE experimental treatment chambers for 3, 12, and 24 months duration beginning in June of 2016 and ending in June of 2018. Analyses of the necromass included pre-incubation chemistry and post-incubation mass loss and Fourier transform infrared (FTIR) spectroscopy of remaining fungal necromass. These data were used in analyses in Fernandez et al (2019). This dataset contains three data files in comma-separate values (*.csv) format. Additional metadata are provided: three data dictionaries and a file-level metadata file in comma-separate values (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES↗

SPRUCE Hyperspectral Remote Sensing of Vegetation Communities in SPRUCE Experimental Plots, 2016

This dataset consists of reflectance observations from hyperspectral remote sensing of the vegetation communities from the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental site within the Marcell Experimental Forest in northern Minnesota, USA. Observations were collected in SPRUCE experimental study plots located in the S1-Bog. Community vegetation plots in ten chambered treatment plots (6, 19, 11, 20, 4, 13, 8, 16, 10, 17) and 3 unchambered ambient plots (7, 14, 21) were scanned. Spectral data were collected on 22 September 2016 (2016-09-22) under clear sky conditions beginning at 1:00 p.m. local time (Central Standard Time). Although it was past peak growing season, senescence had only just begun at the site, and experimentally warmed plots were still near peak growing conditions. Data collection was performed using a PP Systems UniSpec-DC spectroradiometer that detected incoming and reflected solar radiation in 3–4 nm bandwidths between 310 and 1100 nm. The fiber optic cable on the Unispec was fitted with a field of view (FOV) restrictor that limited the FOV to approximately 15°. The instrument foreoptic was leveled at 1 m above the ground capturing an area of approximately 35 cm in diameter, or 0.1 m2. White reference scans were performed between each treatment. Bands at the beginning and end of the spectrum were eliminated due to noise, resulting in a reported data range of 400-1000 nm. These data can help characterize boreal peatland plant composition and species diversity and their responses to elevated temperatures and carbon dioxide concentrations. These observations were used in analyses in McPartland et al (2019). This dataset contains 4 data files in comma-separate values (*.csv) format. Additional metadata are provided: four data dictionaries and a file-level metadata file in comma-separate values (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES↗

SPRUCE High-Resolution Minirhizotrons in an Experimentally-Warmed Peatland Provide an Unprecedented Glimpse at Fine Roots and their Fungal Partners: Supporting Data

Images were collected using first of their kind, non-destructive, high-resolution automated minirhizotrons (RhizoSystems, LLC) to assess the response of plant fine-root and fungal mycelium dynamics to elevated temperatures after 4-6 years of whole-ecosystem warming and exposure to elevated carbon dioxide concentrations (e[CO2]) in a peat bog where the SPRUCE (Spruce and Peatland Responses Under Changing Environments) experiment is located. We focused on two SPRUCE experimental plots: Plot 10 has elevated temperature (+9°C) and plot 19 is a control (+0°C). Both have elevated carbon dioxide (e[CO2]). Changes in root and fungal abundance with warming were estimated from a timeseries of landscape-level mosaiced images for each plot by measuring the proportional abundance of five belowground classes: fine roots of vascular plants, ectomycorrhizas, fungal hyphae, fungal rhizomorphs, and fungal sporocarps. To examine root and fungal phenology responses to warming, the length per individual root or fungal structure (except fungal hyphae that did not grow linearly but rather increased in areal coverage) were measured per image area of a set of timeseries patch-level mosaiced images for each plot. The experimental work was conducted in a Picea mariana [black spruce] – Sphagnum spp. bog forest in northern Minnesota, 40 km north of Grand Rapids, in the USDA Forest Service Marcell Experimental Forest (MEF). This ecosystem, which is located at the southern margin of the boreal forest, is considered especially vulnerable to climate change and anticipated to be near its tipping point. These data were used in analyses published in Defrenne et al (2021). This dataset contains 5 data files in comma-separate values (*.csv) format and a compressed folder (*.zip) containing 383 JPEG (*.jpg) images. Data files contain landscape-level assessment of belowground class abundance, patch-level growth phenology, and environmental variables (originally published in Hanson et al., 2016 and Hanson et al 2020). Images were collected with automated minirhizotrons and analyzed for phenology. Additional metadata are provided: 5 data dictionaries and a file-level metadata file in comma-separate values (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES↗

SPRUCE S1 Bog and Bog Lake Fen Moss Decomposition and Litter Chemistry Data, 2014-2019

This dataset includes measurements of mass loss, decay rate, and litter chemistry of mosses decomposing in the S1 Bog and Bog Lake Fen located in the USDA Forest Service Marcell Experimental Forest (MEF) in northern Minnesota, 40 km north of Grand Rapids. A litterbag experiment was used to estimate decay rates of 3 different moss types (Sphagnum magellanicum, Sphagnum angustifolium/fallax, and Polytrichum sp.) from mass loss measurements after one and five years in hummock and hollow microtopographies in the bog and lawn microtopography in the poor fen. Carbon, nitrogen, and phosphorous contents of the mosses were measured initially, after 1 year, and after 5 years of decay. Sample collection and analyses started in 2014 and was completed in 2019 (2014-06-05 to 2019-06-05). These data are from outside the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental plots. Decomposition data (rates and chemistry changes) can be used to assess ecosystem carbon and nutrient cycles. These data were used in analyses reported in Shelley et al (2022). This dataset contains two data files in comma-separate values (*.csv) format. Additional metadata are provided: two data dictionaries and a file-level metadata file in comma-separate values (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES↗

SPRUCE S1 Bog Production of Newly-Grown Fine Roots Assessed Using Root Ingrowth Cores in 2013

This dataset contains data from root ingrowth cores from the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental site within the Marcell Experimental Forest in northern Minnesota, USA. Root ingrowth cores were used to capture newly-grown fine roots during the 2013 growing season (June - October) at 20 locations at the southern end of the S1-Bog. Rates of fine root production were measured with root ingrowth cores and fine root traits were measured on roots that grew into ingrowth cores. Traits are reported by plant species and root orders and include nitrogen (N) content, root tissue density, specific root length, average diameter. Ingrowth cores were used to compare species-specific depth distribution patterns with minirhizotron analyses and to obtain newly produced roots for chemical analysis and measurement of morphological traits prior to the construction of the SPRUCE experimental plots. This dataset contains two data files in comma-separate values (*.csv) format. Additional metadata are provided: two data dictionaries and a file-level metadata file in comma-separate values (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES↗