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At least 325 records · Page 18
All sourcesNASA NTRSDOE OSTI
DOE OSTI2020

Editorial: Viruses, Genetic Exchange, and the Tree of Life

We live in exciting times for microbiology research. The significant technological and scientific advancements in the past decades have now enabled scientists to pursue discovery and description of novel microbial and viral linages from previously uncharted Earth habitats. Two such discoveries are particularly exciting and noteworthy in this regard. First, the discovery of the first ‘giant virus’, Acanthamoeba polyphaga mimivirus, in 2003, and several others thereafter, posed intriguing questions regarding virus origins, evolution, classification, and their place in the ‘tree of life’. Second, the discoveries of ‘Lokiarchaeota’ and several other closelyrelated archaeal members that encode several eukaryote-specific proteins challenged the threedomain canonical structure of the tree of life. These discoveries have reopened debates on central questions in evolutionary biology research such as the origin of viruses, the origin of eukaryotes, evolutionary relationship between Archaea and Eukarya, and the structure and topology of the tree of life. In this Research Topic, we received a broad range of contributions addressing these and other related questions.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2025

Plant sulfate transporter protein sequences for phylogenetic analysis

Sulfur is an essential macronutrient that supports plant growth, development, and responses to environmental stress. Sulfate is the predominant inorganic form of sulfur in soils, and its uptake by roots and translocation to shoots are facilitated by the sulfate transporter (SULTR) family of proteins. Although the first plant SULTR gene was identified nearly three decades ago, several subfamily members, particularly those in the expansive and angiosperm-specific SULTR3 group, remain poorly characterized. To support comprehensive phylogenetic and sequence-based analyses, we compiled a curated dataset of 262 SULTR protein sequences from 22 plant species spanning the evolutionary breadth of land plants. This collection includes representatives from two basal lineages, two early-divergent angiosperms, six monocots, and ten dicots. All sequences were extracted from genome assemblies available in Phytozome v13 (Joint Genome Institute) and manually curated, with cross-referencing to additional databases such as NCBI when needed. This dataset provides a valuable resource for reconstructing the evolutionary history of the SULTR family, with particular emphasis on the diversification of SULTR3 transporters in flowering plants. This resource may also support functional annotation, comparative genomics, and structural modeling of sulfate transport proteins.

CBI↗
DOE OSTI2022

Temporal partitioning of hatching, maturation, and surface activity by reptiles in Florida longleaf pine-wiregrass sandhills

Temporal partitioning of life history traits among syntopic reptiles can facilitate co-occurrence, but may be influenced by environmental factors and evolutionary history. We used 24 years of continuous capture data in the Florida sandhills to evaluate the timing and duration of hatching, maturation and/or surface activity for ten reptile species, spanning multiple clutch strategies, taxonomic relationships, and habits. We hypothesised: i) species would differ in seasonal timing of hatching and maturation; ii) hatching and maturation periods would be more seasonally-synchronised in fossorial than terrestrial or semi-aquatic reptiles; iii) monthly and annual temperature anomalies would be positively related to hatching, maturation, and surface activity anomalies, and iv) groupings of reptiles by clutch strategy, taxonomic relationship, and habit, would explain more variation in the timing and duration of hatching and maturation than species alone. Seasonal timing of response variables varied widely among species. Hatching peaked for > 1 species during most calendar months. Maturation and surface activity periods ranged from aseasonal to highly-seasonal among species. Hatching began 1.5 months earlier and was more prolonged for terrestrial than fossorial species overall. Hatching peaked in early to mid-summer for terrestrial and fossorial species, and winter for the semi-aquatic Kinosternon subrubrum. Terrestrial and fossorial species did not differ in average timing, duration, or overlap of maturation periods; semi-aquatic Liodytes pygaea matured more consistently across all seasons than other species. Monthly temperature anomalies were negatively correlated with monthly maturation for Plestiodon egregius. Annual temperature and precipitation anomalies were related to annual hatching, maturation, and surface activity trends for several species. Taxonomic relationship, habit, and species explained some variation in hatching and maturation timing and duration. Our results illustrate the influence of environment and evolutionary relationships on the timing of important life history traits. Keywords: Age class, Drift fence, Community, Interspecific, Life history

Zoology↗
DOE OSTI2021

Mitochondrial Genomes of the United States Distribution of Gray Fox (Urocyon cinereoargenteus) Reveal a Major Phylogeographic Break at the Great Plains Suture Zone

We examined phylogeographic structure in gray fox (Urocyon cinereoargenteus) across the United States to identify the location of secondary contact zone(s) between eastern and western lineages and investigate the possibility of additional cryptic intraspecific divergences. We generated and analyzed complete mitochondrial genome sequence data from 75 samples and partial control region mitochondrial DNA sequences from 378 samples to investigate levels of genetic diversity and structure through population- and individual-based analyses including estimates of divergence (FST and SAMOVA), median joining networks, and phylogenies. We used complete mitochondrial genomes to infer phylogenetic relationships and date divergence times of major lineages of Urocyon in the United States. Despite broad-scale sampling, we did not recover additional major lineages of Urocyon within the United States, but identified a deep east-west split (~0.8 million years) with secondary contact at the Great Plains Suture Zone and confirmed the Channel Island fox (Urocyon littoralis) is nested within U. cinereoargenteus. Genetic diversity declined at northern latitudes in the eastern United States, a pattern concordant with post-glacial recolonization and range expansion. Beyond the east-west divergence, morphologically-based subspecies did not form monophyletic groups, though unique haplotypes were often geographically limited. Gray foxes in the United States displayed a deep, cryptic divergence suggesting taxonomic revision is needed. Secondary contact at a common phylogeographic break, the Great Plains Suture Zone, where environmental variables show a sharp cline, suggests ongoing evolutionary processes may reinforce this divergence. Follow-up study with nuclear markers should investigate whether hybridization is occurring along the suture zone and characterize contemporary population structure to help identify conservation units. Comparative work on other wide-ranging carnivores in the region should test whether similar evolutionary patterns and processes are occurring.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2021

Beneath the Bark: Assessing Woody Stem Water and Carbon Fluxes and Its Prevalence Across Climates and the Woody Plant Phylogeny

While woody stems are known to influence carbon and water dynamics, direct exchange with the atmosphere is seldom quantified, limiting our understanding of how these processes influence the exchange of mass and energy. The presence of woody stem chlorophyll in a diversity of climates and across a range of species suggests an evolutionary advantage to sustaining carbon assimilation and water relations through permeable stem tissue. However, no formal evaluation of this hypothesis has been performed. In this mini-review, we explore the interactions between woody stems and the atmosphere by examining woody stem photosynthesis and bark-atmosphere water exchange. Specifically, we address the following questions: (1) How do water and carbon move between the atmosphere and woody stems? (2) In what climate space is woody stem photosynthesis and bark water uptake advantageous? (3) How ubiquitous across plant families is woody stem photosynthesis and bark-atmosphere water exchange? In the literature, only seven species have been identified as exhibiting bark water uptake while over 300 species are thought to conduct woody stem photosynthesis. The carbon dioxide and water gained from these processes can offset respiration costs and improve plant water balance. These species span diverse biomes suggesting a broad prevalence of bark-atmosphere permeability. Finally, our results demonstrate that there may be an evolutionary component as demonstrated by a high Pagel’s lambda for the presence of stem photosynthesis. We end with recommendations for future research that explores how bark water and carbon interactions may impact plant function and mass flow in a changing climate.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2020

Transcriptome Dynamics of the Inflorescence in Reciprocally Formed Allopolyploid Tragopogon miscellus (Asteraceae)

Polyploidy is an important evolutionary mechanism and is prevalent among land plants. Most polyploid species examined have multiple origins, which provide genetic diversity and may enhance the success of polyploids. In some polyploids, recurrent origins can result from reciprocal crosses between the same diploid progenitors. Although great progress has been made in understanding the genetic consequences of polyploidy, the genetic implications of reciprocal polyploidization remain poorly understood, especially in natural polyploids. Tragopogon (Asteraceae) has become an evolutionary model system for studies of recent and recurrent polyploidy. Allotetraploid T. miscellus has formed reciprocally in nature with resultant distinctive floral and inflorescence morphologies (i.e., short- vs. long-liguled forms). In this study, we performed comparative inflorescence transcriptome analyses of reciprocally formed T. miscellus and its diploid parents, T. dubius and T. pratensis. In both forms of T. miscellus, homeolog expression of ~70% of the loci showed vertical transmission of the parental expression patterns (i.e., parental legacy), and ~20% of the loci showed biased homeolog expression, which was unbalanced toward T. pratensis. However, 17.9% of orthologous pairs showed different homeolog expression patterns between the two forms of T. miscellus. No clear effect of cytonuclear interaction on biased expression of the maternal homeolog was found. In terms of the total expression level of the homeologs studied, 22.6% and 16.2% of the loci displayed non-additive expression in short- and long-liguled T. miscellus, respectively. Unbalanced expression level dominance toward T. pratensis was observed in both forms of T. miscellus. Significantly, genes annotated as being involved in pectin catabolic processes were highly expressed in long-liguled T. miscellus relative to the short-liguled form, and the majority of these differentially expressed genes were transgressively down-regulated in short-liguled T. miscellus. Given the known role of these genes in cell expansion, they may play a role in the differing floral and inflorescence morphologies of the two forms. In summary, the overall inflorescence transcriptome profiles are highly similar between reciprocal origins of T. miscellus. However, the dynamic homeolog-specific expression and non-additive expression patterns observed in T. miscellus emphasize the importance of reciprocal origins in promoting the genetic diversity of polyploids.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2024

Is the exquisite specificity of lymphocytes generated by thymic selection or due to evolution?

We have previously argued that the antigen receptors of T and B lymphocytes evolved to be sufficiently specific to avoid massive deletion of clonotypes by negative selection. Their optimal ‘specificity’ level, i.e., probability of binding any particular epitope, was shown to be inversely related to the number of self-antigens that the cells have to be tolerant to. Experiments have demonstrated that T lymphocytes also become more specific during negative selection in the thymus, because cells expressing the most crossreactive receptors have the highest likelihood of binding a self-antigen, and hence to be tolerized (i.e., deleted, anergized, or diverted into a regulatory T cell phenotype). Thus, there are two —not mutually exclusive— explanations for the exquisite specificity of T cells, one involving evolution and the other thymic selection. To better understand the impact of both, we extend a previously developed mathematical model by allowing for T cells with very different binding probabilities in the pre-selection repertoire. We confirm that negative selection tends to tolerize the most crossreactive clonotypes. As a result, the average level of specificity in the functional post-selection repertoire depends on the number of self-antigens, even if there is no evolutionary optimization of binding probabilities. However, the evolutionary optimal range of binding probabilities in the pre-selection repertoire also depends on the number of self-antigens. Species with more self antigens need more specific pre-selection repertoires to avoid excessive loss of T cells during thymic selection, and hence mount protective immune responses. We conclude that both evolution and negative selection are responsible for the high level of specificity of lymphocytes.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

Insights into the Lysine Acetylome of the Haloarchaeon Haloferax volcanii during Oxidative Stress by Quantitative SILAC-Based Proteomics

Oxidative stress adaptation strategies are important to cell function and are linked to cardiac, neurodegenerative disease, and cancer. Representatives of the Archaea domain are used as model organisms based on their extreme tolerance to oxidants and close evolutionary relationship with eukaryotes. A study of the halophilic archaeon Haloferax volcanii reveals lysine acetylation to be associated with oxidative stress responses. The strong oxidant hypochlorite: (i) stimulates an increase in lysine acetyltransferase HvPat2 to HvPat1 abundance ratios and (ii) selects for lysine deacetylase sir2 mutants. Here we report the dynamic occupancy of the lysine acetylome of glycerol-grown H. volcanii as it shifts in profile in response to hypochlorite. These findings are revealed by the: (1) quantitative multiplex proteomics of the SILAC-compatible parent and Δsir2 mutant strains and (2) label-free proteomics of H26 ‘wild type’ cells. The results show that lysine acetylation is associated with key biological processes including DNA topology, central metabolism, cobalamin biosynthesis, and translation. Lysine acetylation targets are found conserved across species. Moreover, lysine residues modified by acetylation and ubiquitin-like sampylation are identified suggesting post-translational modification (PTM) crosstalk. Overall, the results of this study expand the current knowledge of lysine acetylation in Archaea, with the long-term goal to provide a balanced evolutionary perspective of PTM systems in living organisms.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2020

Sequencing and Analysis of the Sex Determination Region of Populus trichocarpa

The ages and sizes of a sex-determination region (SDR) are difficult to determine in non-model species. Due to the lack of recombination and enrichment of repetitive elements in SDRs, the quality of assembly with short sequencing reads is universally low. Unique features present in the SDRs help provide clues about how SDRs are established and how they evolve in the absence of recombination. Several Populus species have been reported with a male heterogametic configuration of sex (XX/XY system) mapped on chromosome 19, but the exact location of the SDR has been inconsistent among species, and thus far, none of these SDRs has been fully assembled in a genomic context. Here we identify the Y-SDR from a Y-linked contig directly from a long-read PacBio assembly of a Populus trichocarpa male individual. We also identified homologous gene sequences in the SDR of P. trichocarpa and the SDR of the W chromosome in Salix purpurea. We show that inverted repeats (IRs) found in the Y-SDR and the W-SDR are lineage-specific. We hypothesize that, although the two IRs are derived from the same orthologous gene within each species, they likely have independent evolutionary histories. Furthermore, the truncated inverted repeats in P. trichocarpa may code for small RNAs that target the homologous gene for RNA-directed DNA methylation. These findings support the hypothesis that diverse sex-determining systems may be achieved through similar evolutionary pathways, thereby providing a possible mechanism to explain the lability of sex-determination systems in plants in general.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2021

Rapid Cis–Trans Coevolution Driven by a Novel Gene Retroposed from a Eukaryotic Conserved CCR4–NOT Component in Drosophila

Young, or newly evolved, genes arise ubiquitously across the tree of life, and they can rapidly acquire novel functions that influence a diverse array of biological processes. Previous work identified a young regulatory duplicate gene in Drosophila, Zeus that unexpectedly diverged rapidly from its parent, Caf40, an extremely conserved component in the CCR4–NOT machinery in post-transcriptional and post-translational regulation of eukaryotic cells, and took on roles in the male reproductive system. This neofunctionalization was accompanied by differential binding of the Zeus protein to loci throughout the Drosophila melanogaster genome. However, the way in which new DNA-binding proteins acquire and coevolve with their targets in the genome is not understood. Here, by comparing Zeus ChIP-Seq data from D. melanogaster and D. simulans to the ancestral Caf40 binding events from D. yakuba, a species that diverged before the duplication event, we found a dynamic pattern in which Zeus binding rapidly coevolved with a previously unknown DNA motif, which we term Caf40 and Zeus-Associated Motif (CAZAM), under the influence of positive selection. Interestingly, while both copies of Zeus acquired targets at male-biased and testis-specific genes, D. melanogaster and D. simulans proteins have specialized binding on different chromosomes, a pattern echoed in the evolution of the associated motif. Using CRISPR-Cas9-mediated gene knockout of Zeus and RNA-Seq, we found that Zeus regulated the expression of 661 differentially expressed genes (DEGs). Our results suggest that the evolution of young regulatory genes can be coupled to substantial rewiring of the transcriptional networks into which they integrate, even over short evolutionary timescales. Our results thus uncover dynamic genome-wide evolutionary processes associated with new genes.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

Expanded Archaeal Genomes Shed New Light on the Evolution of Isoprenoid Biosynthesis

Isoprenoids and their derivatives, essential for all cellular life on Earth, are particularly crucial in archaeal membrane lipids, suggesting that their biosynthesis pathways have ancient origins and play pivotal roles in the evolution of early life. Despite all eukaryotes, archaea, and a few bacterial lineages being known to exclusively use the mevalonate (MVA) pathway to synthesize isoprenoids, the origin and evolutionary trajectory of the MVA pathway remain controversial. Here, we conducted a thorough comparison and phylogenetic analysis of key enzymes across the four types of MVA pathway, with the particular inclusion of metagenome assembled genomes (MAGs) from uncultivated archaea. Our findings support an archaeal origin of the MVA pathway, likely postdating the divergence of Bacteria and Archaea from the Last Universal Common Ancestor (LUCA), thus implying the LUCA’s enzymatic inability for isoprenoid biosynthesis. Notably, the Asgard archaea are implicated in playing central roles in the evolution of the MVA pathway, serving not only as putative ancestors of the eukaryote- and Thermoplasma-type routes, but also as crucial mediators in the gene transfer to eukaryotes, possibly during eukaryogenesis. Overall, this study advances our understanding of the origin and evolutionary history of the MVA pathway, providing unique insights into the lipid divide and the evolution of early life.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2020

Biosystems Design to Accelerate C 3 -to-CAM Progression

Global demand for food and bioenergy production has increased rapidly, while the area of arable land has been declining for decades due to damage caused by erosion, pollution, sea level rise, urban development, soil salinization, and water scarcity driven by global climate change. In order to overcome this conflict, there is an urgent need to adapt conventional agriculture to water-limited and hotter conditions with plant crop systems that display higher water-use efficiency (WUE). Crassulacean acid metabolism (CAM) species have substantially higher WUE than species performing C 3 or C 4 photosynthesis. CAM plants are derived from C 3 photosynthesis ancestors. However, it is extremely unlikely that the C 3 or C 4 crop plants would evolve rapidly into CAM photosynthesis without human intervention. Currently, there is growing interest in improving WUE through transferring CAM into C 3 crops. However, engineering a major metabolic plant pathway, like CAM, is challenging and requires a comprehensive deep understanding of the enzymatic reactions and regulatory networks in both C 3 and CAM photosynthesis, as well as overcoming physiometabolic limitations such as diurnal stomatal regulation. Recent advances in CAM evolutionary genomics research, genome editing, and synthetic biology have increased the likelihood of successful acceleration of C 3 -to-CAM progression. Here, we first summarize the systems biology-level understanding of the molecular processes in the CAM pathway. Then, we review the principles of CAM engineering in an evolutionary context. Lastly, we discuss the technical approaches to accelerate the C 3 -to-CAM transition in plants using synthetic biology toolboxes.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2020

Spitzer Variability Properties of Low-gravity L Dwarfs

We present Spitzer Space Telescope variability monitoring observations of three low-gravity L dwarfs with previous detections of variability in the near-IR: 2MASS J0045+16, 2MASS J0501–00, and 2MASS J1425–36. We detect significant periodic variability in two of our targets, 2MASS J0045+16 and 2MASS J0501–00. We do not detect variability in 2MASS J1425–36. Combining our new rotation periods with rotational velocities, we calculate inclination angles of 22° ± 1°, 60{sub -8}{sup +13°}, and 52{sub -13}{sup +19°} for 2MASS J0045+16, 2MASS J0501–00, and 2MASS J1425–36, respectively. Our three new objects are consistent with the tentative relations between inclination, amplitude, and color anomaly previously reported. Objects with the highest variability amplitudes are inclined equator on, while the maximum observed amplitude decreases as the inclination angle decreases. We also find a correlation between the inclination angle and (J–K){sub 2MASS} color anomaly for the sample of objects with measured inclinations. Compiling the entire sample of brown dwarfs with Spitzer variability detections, we find no enhancement in amplitude for young, early-L dwarfs compared to the field dwarf population. We find a possible enhancement in amplitude of low-gravity late-L dwarfs at 4.5 μm. We do not find a correlation between amplitude ratio and spectral type for field dwarfs or for the young population. Finally, we compile the rotation periods of a large sample of brown dwarfs with ages 1 Myr–1 Gyr and compare the rotation rates predicted by evolutionary models assuming angular momentum conservation. We find that the rotation rates of the current sample of brown dwarfs fall within the expected range set by evolutionary models and breakup limits.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2020

The Outburst of the Young Star Gaia19bey

We report photometry and spectroscopy of the outburst of the young stellar object Gaia19bey. We have established the outburst light curve with archival Gaia “G,” ATLAS “Orange,” Zwicky Transient Facility r-band, and Pan-STARRS “rizy”-filter photometry, showing an outburst of ~4 yr duration, longer than typical EX Lupi objects but shorter than FU Orionis objects. Its pre-outburst spectral energy distribution shows a flat far-infrared spectrum, confirming the early evolutionary state of Gaia19bey and its similarity to other deeply embedded young stars experiencing outbursts. A lower limit to the peak outburst luminosity is ~182 L{sub ⊙} at an assumed distance of 1.4 kpc, the minimum plausible distance. Infrared and optical spectroscopy near maximum light show an emission line spectrum, including H i lines, strong red Ca ii emission, other metal emission lines, infrared CO bandhead emission, and a strong infrared continuum. Toward the end of the outburst, the emission lines have all but disappeared and the spectrum has changed into an almost pure continuum spectrum. This indicates a cessation of magnetospheric accretion activity. The near-infrared colors have become redder as Gaia19bey has faded, indicating a cooling of the continuum component. Near the end of the outburst, the only remaining strong emission lines are forbidden shock-excited emission lines. Adaptive optics integral-field spectroscopy shows the H{sub 2} 1–0 S(1) emission with the morphology of an outflow cavity and the extended emission in the [Fe ii] line at 1644 nm with the morphology of an edge-on disk. However, we do not detect any large-scale jet from Gaia19bey.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2020

Near-infrared Characterization of Four Massive Stars in Transition Phases

Massive stars typically undergo short-lived post-main-sequence evolutionary phases with strong mass loss and occasional mass eruptions. Many of such massive stars in transition phases have been identified based on their dusty envelopes. The ejected material often veils the stellar photospheres so that the central stars cannot be assigned proper spectral types and evolutionary stages. The infrared spectral range has proved to be ideal for the classification of evolved massive stars and for the characterization of their environments. To improve our knowledge on the central stars of four such dust-enshrouded objects, [GKF 2010] MN 83, [GKF 2010] MN 108, [GKF 2010] MN 109, and [GKF 2010] MN 112, we collect and present their first medium-resolution K-band spectra in the 2.3–2.47 μm region and discuss the location of the stars in the JHK color–color diagram. We find that the emission-line spectra of both MN 83 and MN 112 show characteristics typically seen in luminous blue variable (LBV) stars. In addition, we propose that the presence and strength of the newly reported Mg ii lines might be used as a new complementary criterion to identify LBV candidates. The spectra of the other two objects imply that MN 108 is an O-type supergiant, whereas MN 109 could be an LBV candidate in its active phase. We derive lower limits for the reddening toward the stars and find that three of all dereddened fall into the region of confirmed LBVs.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2020

Kepler Binary Stars in the NGC 6819 Open Cluster: KIC 5113146 and KIC 5111815

In this study, an investigation of two double-lined binary stars KIC 5113146 and KIC 5111815 in the NGC 6819 open cluster is presented based on both photometric and spectroscopic data. Simultaneous analysis of light and radial velocity curves was made and the absolute parameters of the systems’ components were determined for the first time. We find that both systems have F-type main-sequence components. The masses and radii were found to be M 1 = 1.29 ± 0.02M ⊙ , R 1 = 1.47 ± 0.03 R ⊙ and M 2 = 1.19 ± 0.02 M ⊙ , R 2 = 1.13 ± 0.02 R ⊙ for the primary and secondary components of KIC 5113146; and M 1 = 1.51 ± 0.08 M ⊙ , R 1 = 2.02 ± 0.05 R ⊙ and M 2 = 1.19 ± 0.07 M ⊙ , R 2 = 1.32 ± 0.04 R ⊙ for the components of KIC 5111815, respectively. The evolutionary status of the components was evaluated based on the MESA evolutionary tracks and isochrones. The ages of KIC 5111815 and KIC 5113146 were derived to be about 2.50 ± 0.35 Gyr and 1.95 ± 0.40 Gyr, respectively. Photometric distances were calculated to be 2850 ± 185 pc for KIC 5113146 and 3120 ± 260 pc for KIC 5111815. The results reveal that both KIC 5113146 and KIC 5111815 systems are the most likely members of NGC 6819.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2021

The Red Supergiant Content of M31 and M33

We identify red supergiants (RSGs) in our spiral neighbors M31 and M33 using near-IR (NIR) photometry complete to a luminosity limit of logL/L{sub ⊙}=4.0. Our archival survey data cover 5 deg{sup 2} of M31, and 3 deg{sup 2} for M33, and are likely spatially complete for these massive stars. Gaia is used to remove foreground stars, after which the RSGs can be separated from asymptotic giant branch (AGB) stars in the color–magnitude diagram. The photometry is used to derive effective temperatures and bolometric luminosities via MARCS stellar atmosphere models. The resulting H-R diagrams show superb agreement with the evolutionary tracks of the Geneva evolutionary group. Our census includes 6400 RSGs in M31 and 2850 RSGs in M33 within their Holmberg radii; by contrast, only a few hundred RSGs are known so far in the Milky Way. Our catalog serves as the basis for a study of the RSG binary frequency being published separately, as well as future studies relating to the evolution of massive stars. Here we use the matches between the NIR-selected RSGs and their optical counterparts to show that the apparent similarity in the reddening of OB stars in M31 and M33 is the result of Malmquist bias; the average extinction in M31 is likely higher than that of M33. As expected, the distribution of RSGs follows that of the spiral arms, while the much older AGB population is more uniformly spread across each galaxy’s disk.

47 OTHER INSTRUMENTATION↗
DOE OSTI2021

TOI-811b and TOI-852b: New Transiting Brown Dwarfs with Similar Masses and Very Different Radii and Ages from the TESS Mission

We report the discovery of two transiting brown dwarfs (BDs), TOI-811b and TOI-852b, from NASA’s Transiting Exoplanet Survey Satellite mission. These two transiting BDs have similar masses but very different radii and ages. Their host stars have similar masses, effective temperatures, and metallicities. The younger and larger transiting BD is TOI-811b at a mass of M {sub b} = 59.9 ± 13.0M {sub J} and radius of R {sub b} = 1.26 ± 0.06R {sub J}, and it orbits its host star in a period of P = 25.16551 ± 0.00004 days. We derive the host star’s age of 93{sub −29}{sup +61} Myr from an application of gyrochronology. The youth of this system, rather than external heating from its host star, is why this BD’s radius is relatively large. This constraint on the youth of TOI-811b allows us to test substellar mass–radius evolutionary models at young ages where the radius of BDs changes rapidly. TOI-852b has a similar mass at M {sub b} = 53.7 ± 1.4M {sub J} but is much older (4 or 8 Gyr, based on bimodal isochrone results of the host star) and is also smaller with a radius of R {sub b} = 0.83 ± 0.04R {sub J}. TOI-852b’s orbital period is P = 4.94561 ± 0.00008 days. TOI-852b joins the likes of other old transiting BDs that trace out the oldest substellar mass–radius evolutionary models where contraction of the BD’s radius slows and approaches a constant value. Both host stars have a mass of M {sub ⋆} = 1.32M {sub ⊙} ± 0.05 and differ in their radii, T {sub eff}, and [Fe/H], with TOI-811 having R {sub ⋆} = 1.27 ± 0.09R {sub ⊙}, T {sub eff} = 6107 ± 77 K, and [Fe/H] = + 0.40 ± 0.09 and TOI-852 having R {sub ⋆} = 1.71 ± 0.04R {sub ⊙}, T {sub eff} = 5768 ± 84 K, and [Fe/H] = + 0.33 ± 0.09. We take this opportunity to examine how TOI-811b and TOI-852b serve as test points for young and old substellar isochrones, respectively.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗