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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 325 records · Page 18
All sourcesNASA NTRSDOE OSTI
DOE OSTI2020

Linking the Dynamic Response of the Carbon Dioxide-Concentrating Mechanism to Carbon Assimilation Behavior in Fremyella diplosiphon

ABSTRACT Cyanobacteria use a carbon dioxide (CO 2 )-concentrating mechanism (CCM) that enhances their carbon fixation efficiency and is regulated by many environmental factors that impact photosynthesis, including carbon availability, light levels, and nutrient access. Efforts to connect the regulation of the CCM by these factors to functional effects on carbon assimilation rates have been complicated by the aqueous nature of cyanobacteria. Here, we describe the use of cyanobacteria in a semiwet state on glass fiber filtration discs—cyanobacterial discs—to establish dynamic carbon assimilation behavior using gas exchange analysis. In combination with quantitative PCR (qPCR) and transmission electron microscopy (TEM) analyses, we linked the regulation of CCM components to corresponding carbon assimilation behavior in the freshwater, filamentous cyanobacterium Fremyella diplosiphon . Inorganic carbon (C i ) levels, light quantity, and light quality have all been shown to influence carbon assimilation behavior in F. diplosiphon . Our results suggest a biphasic model of cyanobacterial carbon fixation. While behavior at low levels of CO 2 is driven mainly by the C i uptake ability of the cyanobacterium, at higher CO 2 levels, carbon assimilation behavior is multifaceted and depends on C i availability, carboxysome morphology, linear electron flow, and cell shape. Carbon response curves (CRCs) generated via gas exchange analysis enable rapid examination of CO 2 assimilation behavior in cyanobacteria and can be used for cells grown under distinct conditions to provide insight into how CO 2 assimilation correlates with the regulation of critical cellular functions, such as the environmental control of the CCM and downstream photosynthetic capacity. IMPORTANCE Environmental regulation of photosynthesis in cyanobacteria enhances organismal fitness, light capture, and associated carbon fixation under dynamic conditions. Concentration of carbon dioxide (CO 2 ) near the carbon-fixing enzyme RubisCO occurs via the CO 2 -concentrating mechanism (CCM). The CCM is also tuned in response to carbon availability, light quality or levels, or nutrient access—cues that also impact photosynthesis. We adapted dynamic gas exchange methods generally used with plants to investigate environmental regulation of the CCM and carbon fixation capacity using glass fiber-filtered cells of the cyanobacterium Fremyella diplosiphon . We describe a breakthrough in measuring real-time carbon uptake and associated assimilation capacity for cells grown in distinct conditions (i.e., light quality, light quantity, or carbon status). These measurements demonstrate that the CCM modulates carbon uptake and assimilation under low-C i conditions and that light-dependent regulation of pigmentation, cell shape, and downstream stages of carbon fixation are critical for tuning carbon uptake and assimilation.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2025

Durable, Ultra-Thin Diaphragms for Liquid Alkaline Water Electrolysis

W. L. Gore & Associates assembled a world class team (De Nora Tech, LLC; New York University; and thyssenkrupp nucera) to develop durable, ultra-low resistance diaphragms that dramatically improve system efficiency, dynamic response, and operational flexibility. To enable the use of ultra-thin diaphragms, an optimized electrode package was chosen as part of an electrode down select study. Ultra-thin diaphragms based on microporous polymer scaffolds imbibed with zirconia nanoparticles were developed and tested. Performance and durability on par with incumbent materials was demonstrated. A transparent electrolyzer to directly observe in-situ diaphragm-bubble interactions was designed and built, and simultaneous optical and electrochemical performance tests were performed. A Pressurized Alkaline Water Electrolysis (PAWE) single cell test station was designed to support advanced R&D in high-pressure electrolysis, particularly for evaluating novel electrode and separator configurations under elevated temperature and pressure operating conditions. Due to early project termination, the original scope could not be fully achieved.

08 HYDROGEN↗
NASA NTRS1972

Experimental study of flow distribution with circumferential manifolds

Water flow test results on fluid flow distribution and pressure loss in curved manifolds with tangential or radial entry are reported. Manifolds were studied both as inlet and outlet manifolds. Manifolds can be used for boilers and/or heat exchangers for advanced space electric power plants.

Dittrich, R. T.↗
NASA NTRS1974

Control vane for engine exhaust flow

Vane solves problem of hot-gas exhaust impingement on curved-duct exteriors, shielding, and other nearby structure components. To eliminate secondary egress of exhaust, curved vane is placed upstream of engine exhaust duct and in close proximity to exhaust exit to induce free-stream flow more in line with exit.

Shaw, C. S.↗
NASA NTRS1975

The effect of grazing flow on the steady state resistance of square-edged orifices

A simple inviscid interaction model has been developed which uses thin airfoil theory to account for pressure forces exerted at the interface between the orifice and grazing flow together with a one-dimensional discharge coefficient concept. The effect of grazing flow boundary layer thickness was also included in the model. Resistance measurements for a wide range of grazing flow speeds and orifice flow rates collapse into a single curve when plotted in terms of effective discharge coefficient versus orifice-to-grazing velocity ratio. The correlation curves for inflow and outflow are different. Data for clustered orifices collapse in the same way as the single orifice. The effect of boundary layer thickness is compared with model predictions. The effect of orifice length-diameter ratio is shown to be significant for inflow but negligible for outflow.

Rogers, T.↗
NASA NTRS1976

Effect of grazing flow on steady-state resistance of isolated square-edged orifices

Steady state diagnostic testing of an isolated orifice has shown the nature of the interaction between grazing and orifice flow causing the large increase in orifice resistance for both inflow and outflow. A simple inviscid interaction model is developed which uses thin aerofoil theory to account for pressure forces exerted at the interface between the orifice and grazing flow together with a one-dimensional discharge coefficient concept. The effect of grazing flow boundary layer thickness is also included in the model. Resistance measurements for each orifice tested, over a wide range of grazing flow speeds and flow rates, collapse into a single curve when plotted in terms of effective discharge coefficient against orifice to grazing velocity ratio. The correlation curves for inflow and outflow are different. Data for clustered orifices collapse in the same way as those for the single orifice. The effect of boundary layer thickness is compared with model predictions.

Rogers, T.↗
NASA NTRS1979

An efficient user-oriented method for calculating compressible flow in an about three-dimensional inlets

A panel method is used to calculate incompressible flow about arbitrary three-dimensional inlets with or without centerbodies for four fundamental flow conditions: unit onset flows parallel to each of the coordinate axes plus static operation. The computing time is scarcely longer than for a single solution. A linear superposition of these solutions quite rigorously gives incompressible flow about the inlet for any angle of attack, angle of yaw, and mass flow rate. Compressibility is accounted for by applying a well-proven correction to the incompressible flow. Since the computing times for the combination and the compressibility correction are small, flows at a large number of inlet operating conditions are obtained rather cheaply. Geometric input is aided by an automatic generating program. A number of graphical output features are provided to aid the user, including surface streamline tracing and automatic generation of curves of curves of constant pressure, Mach number, and flow inclination at selected inlet cross sections. The inlet method and use of the program are described. Illustrative results are presented.

Hess, J. L.↗
NASA NTRS1981

Investigation of aerodynamic characteristics of wings having vortex flow using different numerical codes

The aerodynamic characteristics of highly sweptback wings having separation-induced vortex flow were investigated by employing different numerical codes with a view to determining some of the capabilities and limitations of these codes. Flat wings of various configurations-strake wing models, cropped, diamond, arrow and double delta wings, were studied. Cambered and cranked planforms have also been tested. The theoretical results predicted by the codes were compared with the experimental data, wherever possible, and found to agree favorably for most of the configurations investigated. However, large cambered wings could not be successfully modeled by the codes. It appears that the final solution in the free vortex sheet method is affected by the selection of the initial solution. Accumulated span loadings estimated for delta and diamond wings were found to be unusual in comparison with attached flow results in that the slopes of these load curves near the leading edge do not tend to infinity as they do in the case of attached flow.

Reddy, C. S.↗
NASA NTRS1982

On the role of successive downstream development in East Asian polar air outbreaks

Common features were drawn from 16 events of wintertime migration of cold Siberian air moving southeastward across the east Asia coast, accompanied by strong northerly winds. Criteria for including an event as an instance of a typical synoptic scale occurrence comprised a surface pressure gradient over Korea exceeding 2.5 mb/100 km, and a drop in the daily mean temperature of over 5 C in one day. The events were required to have at least a 10 day separation. A sequence of events was discerned, including the formation of troughs and ridges over the western north Atlantic 6-7 days before an event, their development and decay downstream from one another across the Eurasian continent, and then an outbreak of polar weather. The troughs and ridges displayed maximum amplitude in the same places in the majority of cases studied, with the center moving along a curved trajectory of the 300 mb flow at nearly 30 deg longitudinally every day.

Jung, C. H.↗
NASA NTRS1983

Analytical prediction of aerothermal environment in a wing-elevon cove

The fluid/thermal environment in the cove between the wing and elevon surfaces was a concern throughout the design and initial operational phases of the space shuttle. Experimenal and analytical investigations provided some understanding of the environmental in the wing-elevon cove. An incompressible finite element analysis of flow through straight parallel walls and curved parallel walls to determine the effects of cove geomtry on the fluid/thermal environment is described. Results from this analysis agree qualitatively with experimental data. The centerline gas temperatures and cold wall heating rates are virtually identical for the two cases indicating that the slight curvature has little effect on the overall thermal environment.

Bey, K. S.↗
NASA NTRS1988

Ionisation cross sections of rare-gas atoms by electron impact

A pulsed electron beam and ion extraction method is used to measure normalized values of partial ionization cross sections for rare gases from threshold to 1000 eV. Cross sections obtained for singly ionized species are used to calibrate the mass transmission efficiency of the ion extraction/analyzer/detection system by the relative flow technique, and this mass transmission curve is then used to determine the absolute cross sections of the multiply ionized species. Total ion cross sections are found by summation of the individual partial cross sections with proper weighting for charge.

Krishnakumar, E.↗
NASA NTRS1961

Cavitation Performance of a Centrifugal Pump with Water and Mercury

The cavitation performance of a given centrifugal pump with water (hot and cold) and mercury is compared. It is found that there are significant scale effects with all fluids tested, with the Thoma cavitation parameter decreasing in all cases for increased pump speed or fluid Reynolds' number. The data for a fixed flow coefficient fall into a single curve when plotted against pump speed (or fluid velocity), rather than against Reynolds' number. Conversely, the Thoma parameter for a given Reynolds' number is approximately twice as large for mercury as for water. The direction of this variation is as predicted from consideration of the cavitation thermodynamic parameters which vary by a factor of 10(exp 7) between these fluids. No difference in cavitation performance between hot and cold water (approximately 160 F and 80 F) was observed, However, the thermodynamic parameters vary only by a factor of 5.

Hammitt, F. G.↗
NASA NTRSDate not supplied

Characterization of CO 4th Positive Radiation in CO2-Ar Mixtures

This paper presents measurements and simulations of CO 4th Positive spectra obtained in NASA Ames Research Center's Electric Arc Shock Tube (EAST) and CentraleSupelec's Plasma Torch. The experiments were aimed at measuring optically thin emission for the main band features of CO 4th Positive by using test gas mixtures of CO2 and Ar. By using mixtures of CO2 and Ar, the temperature of the flow (and consequently black-body curve) is increased compared to using pure CO2 (or CO2 / N2 mixtures). This temperature increase allows for emission spectroscopy of features that would nominally be black-body limited for conditions relevant to Mars entry. The CO 4th Positive system is the focus of this study as it accounts for a large percentage of the emitted radiation for Martian entry, and also due to the difficulties of obtaining experimental validation data due to the emission appearing in the Vacuum Ultraviolet (VUV) spectral range. The focus of this paper is to provide a comprehensive comparison between the EAST and plasma torch data along with various CO 4th Positive databases available in the literature. Previously reported VUV CO2 / N2 EAST data required a larger temperature than would be predicted based upon equilibrium shock relations. The need for the temperature increase implies that the EAST experiment did not fully equilibrate. Thus, it is presumed that the EAST data did not obtain an equilibrium condition. The plasma torch data, on the other hand, is very consistent with equilibrium assumptions. Thus, the equilibrium data obtained in the plasma torch is utilized to construct the CO 4th Positive emission model, which is then applied to the EAST data with a non-equilibrium interpretation.

A. M. Brandis↗
NASA NTRS1986

Mach reflection and aerodynamic choking in two-dimensional ducted flow

Flow through a two-dimensional duct with supersonic inflow is numerically investigated, from the viewpoint of the formation of Mach reflection, aerodynamic choking, and the possibility of constructing a curve similar to that for the quasi-one-dimensional flow in a converging-diverging duct. Such a curve can be used to determine whether a duct with a certain area ratio will or will not choke for a given inflow Mach number. Plots of pressure and mass flux contours are obtained for a given duct configuration. It is found that the two-dimensional flow always chokes at a higher Mach number than the corresponding quasi-one-dimensional flow for a given throat/inlet flow area ratio.

Kumar, A.↗
NASA NTRS1988

Vortex shedding experiment with flat and curved bluff plates in water

Vortex shedding experiments were conducted in a water flow facility in order to simulate the strong discrete 4000-Hz vibration detected in the Space Shuttle Main Engine (SSME) which is thought to be associated with the SSME LOX inlet tee splitter vanes on the Main Injector. For the case of a flat vane with a blunt trailing edge excited by flow induced vortex shedding, lock-in with the first bending mode of the plate was observed. A curved vane displayed similar behavior, with the lock-in being a more discrete higher amplitude response. Aluminum vanes were employed to decouple the first vane bending mode from the vortex shedding mode. The application of an asymmetric 30-deg trailing edge bevel to both the flat and curved vanes was found to greatly reduce the strength of the shed vortices.

Reed, D.↗
NASA NTRS1987

Numerical simulation by TVD schemes of complex shock reflections from airfoils at high angle of attack

The shock-capturing capability of total variation diminishing (TVD) schemes is demonstrated for a more realistic complex shock-diffraction problem for which the experimental data are available. Second-order explicit upwind and symmetric TVD schemes are used to solve the time-dependent Euler equations of gas dynamics for the interaction of a blast wave with an airfoil at high angle-of-attack. The test cases considered are a time-dependent moving curved-shock wave and a contant moving planar-shock wave impinging at an angle-of-attack 30 deg on a NACA 0018 airfoil. Good agreement is obtained between isopycnic contours computed by the TVD schemes and those from experimental interferograms. No drastic difference in flow-field structure is found between the curved- and planar-shock wave cases, except for a difference in density level near the lower surface of the airfoil. Computation for cases with higher shock Mach numbers is also possible. Numerical experiments show that the symmetric TVD scheme is less sensitive to the boundary conditions treatment than the upwind scheme.

Moon, Young J.↗