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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 325 records · Page 18
All sourcesNASA NTRSDOE OSTI
NASA NTRS2016

Comparison of Simultaneous PIV and Hydroxyl Tagging Velocimetry in Low Velocity Flows

Hydroxyl tagging velocimetry (HTV) is a molecular tagging velocimetry (MTV) technique that relies on the photo- dissociation of water vapor into OH radicals and their subsequent tracking using laser-induced fluorescence. At ambient temperature in air, the OH species lifetime is about 50 micro-s. The feasibility of using HTV for probing low- speed flows (a few m/s) is investigated by using an inert, heated gas as a means to increase the OH species lifetime. Unlike particle-based techniques, MTV does not suffer from tracer settling, which is particularly problematic at low speeds. Furthermore, the flow needs to be seeded with only a small mole fraction of water vapor, making it safer for both the user and facilities than other MTV techniques based on corrosive or toxic chemical tracers. HTV is demonstrated on a steam-seeded nitrogen jet at approximately 75 C in the laminar (Umean=3.31 m/s, Re=1,540), transitional (Umean=4.48 m/s, Re=2,039), and turbulent (Umean=6.91 m/s, Re=3,016) regimes at atmospheric pressure. The measured velocity profiles are compared with particle image velocimetry (PIV) measurements performed simultaneously with a second imager. Seeding for the PIV is achieved by introducing micron-sized water droplets into the flow with the steam; the same laser sheet is used for PIV and HTV to guarantee spatial and temporal overlap of the data. Optimizing each of these methods, however, requires conflicting operating conditions: higher temperatures benefit the HTV signals but reduce the available seed density for the PIV through evaporation. Nevertheless, data are found to agree within 10% for the instantaneous velocity profiles and within 5% for the mean profiles and demonstrate the feasibility of HTV for low-speed flows at moderate to high temperatures.

Andre, Matthieu A.↗
NASA NTRS2022

Determination of Chlorophyll Content in Selected Grass Communities of Krkonoše Mts. Tundra Based on Laboratory Spectroscopy and Aerial Hyperspectral Data

The study focuses on the determination of chlorophyll content in four prevailing grasses in the relict arctic-alpine tundra located in the Krkonoše Mountains National Park, Czech Republic. We compared two methods for determination of leaf chlorophyll content (LCC) – spectrophotometric determination in the laboratory, and the LCC assessed by fluorescence portable chlorophyll meter CCM-300. Relationships were established between the LCCs and vegetation indices calculated from leaf spectra acquired with contact probe coupled with an ASD FieldSpec4 Wide-Res spectroradiometer. Canopy chlorophyll contents (CCC) were computed from the LCCs and green leaf area index (LAI), and modelled based on the field spectra measured by the spectroradiometer and the hyperspectral images acquired by Headwall Nano-Hyperspec® mounted on the DJI Matrice 600 Pro drone. The calculations are performed on datasets acquired in June, July and August 2020 together and separately for species and months. In general, the correlations based on June datasets work the best at both levels: median R 2 for all indices was 0.52 for all species together at leaf level and median R 2 = 0.47 at the canopy level (vegetation indices computed from field spectra). Canopy chlorophyll content map was created based on the results of stepwise multiple linear regression. The R 2 was 0.42 when using four wavelengths from the red and red edge spectral region. We attribute the weak model performance to a combination of several factors: leaf structure may bias LCC from laboratory measurements, effects of LAI variability on CCC, and the sampling design, probably not covering the whole phenology equally for all studied species.

L. Cervena↗
NASA NTRSDate not supplied

Field Tests With Trident Drill in Bishop Tuff Help Prepare for Future Missions to Moon and Mars

We performed drilling in volcanic deposits near Bishop California using an engineering model of the Honeybee Robotics TRIDENT (The Regolith and Ice Drill for Exploration of New Terrains) drill [1] a rotary percussive 1-meter class drill that is carried on the PRIME1and VIPER (Volatiles Investigating Polar Exploration Rover)[2] missions that launch in 2024. A similar drilling system was planned for the proposed Icebreaker Discovery class mission to Mars [3] and the Mars Life Explorer mission recommended by the 2020 Decadal Survey of planetary science [4]. The objectives of the project were (1) to use data collected by the drill for operational purposes as a probe of subsurface material properties in formations that are analogous to those that may be encountered on planetary surfaces; (2) correlate subsurface structures with those deduced from Ground Penetrating Radar (GPR); and (3) inspect the boreholes after they were drilled to test PERISCOPE (Probe for Exploring Regolith and Ice by Subsurface Classification of Organics, polycyclic aromatic hydrocarbons (PAHs), and Elements), a newly developed downhole UV fluorescence spectrometer [5].

Carol R. Stoker↗
DOE OSTI2025

Spatial profile of argon (1s 5 ) metastables in an electron beam generated plasma

Electron beams with an applied magnetic field generate a secondary cold plasma with a selective chemical composition, featuring low-energy ions and metastable species in the discharge periphery, ideal for low-damage plasma treatment of material substrates. In this work, we studied the plasma generated by an e-beam using a 4 kV voltage in a pure argon gas environment under a magnetic field of 150 G and in the pressure range of 25–90 mTorr. We measured the absolute spatial density profile of argon (1 s 5 ) metastables in an electron beam generated plasma by laser-induced fluorescence and found it to be of the order of 10 16 m −3 . The electron temperature and the electron density measured by a Langmuir probe were of the order of 10 16 m −3 and less than an eV respectively. Electron-impact quenching was identified as a significant loss mechanism for the Ar(1s 5 ) state, leading to the saturation of the metastable density at higher pressures. Outside the primary ionization region, the spatial distribution of argon metastables followed a linear diffusion profile, indicating negligible additional production in those regions.

EEDF↗
DOE OSTI2021

Development of a cosmic ray oriented trigger for the fluorescence telescope on EUSO-SPB2

The Extreme Universe Space Observatory on a Super Pressure Balloon 2 (EUSO-SPB2), in preparation, aims to make the first observations of Ultra-High Energy Cosmic Rays (UHECRs) from near space using optical techniques. EUSO-SPB2 will prototype instrumentation for future satellite-based missions, including the Probe of Extreme Multi-Messenger Astrophysics (POEMMA) and K-EUSO. The payload will consist of two telescopes. The first is a Cherenkov telescope (CT) being developed to quantify the background for future below-the-limb very high energy (E 10 PeV) astrophysical neutrino observations, and the second is a fluorescence telescope (FT) being developed for detection of UHECRs. The FT will consist of a Schmidt telescope, and a 6192 pixel ultraviolet camera with an integration time of 1.05 s. The first step in the data acquisition process for the FT is a hardware level trigger in order to decide which data to record. In order to maximize the number of UHECR induced extensive air showers (EASs) which can be detected, a novel trigger algorithm has been developed based on the intricacies and limitations of the detector. Finally, the expected performance of the trigger has been characterized by simulations and, pending hardware verification, shows that EUSO-SPB2 is well positioned to attempt the first near-space observation of UHECRs via optical techniques.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2024

Radio measurements of the depth of air-shower maximum at the Pierre Auger Observatory

The (AERA), part of the Pierre Auger Observatory, is currently the largest array of radio antenna stations deployed for the detection of cosmic rays, spanning an area of 17 km 2 with 153 radio stations. It detects the radio emission of extensive air showers produced by cosmic rays in the 30–80 MHz band. Here, we report the AERA measurements of the ( X max ), a probe for mass composition, at cosmic-ray energies between 10 17.5 and 10 18.8 eV , which show agreement with earlier measurements with the fluorescence technique at the Pierre Auger Observatory. We show advancements in the method for radio X max reconstruction by comparison to dedicated sets of / air-shower simulations, including steps of reconstruction-bias identification and correction, which is of particular importance for irregular or sparse radio arrays. Using the largest set of radio air-shower measurements to date, we show the radio X max resolution as a function of energy, reaching a resolution better than 15 g cm − 2 at the highest energies, demonstrating that radio X max measurements are competitive with the established high-precision fluorescence technique. In addition, we developed a procedure for performing an extensive data-driven study of systematic uncertainties, including the effects of acceptance bias, reconstruction bias, and the investigation of possible residual biases. These results have been cross-checked with air showers measured independently with both the radio and fluorescence techniques, a setup unique to the Pierre Auger Observatory. Published by the American Physical Society 2024

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
NASA NTRS2004

Ultrasensitive Label-free Electronic Chip for DNA Analysis Using Carbon Nanotube Nanoelectrode Arrays

There is a strong need for faster, cheaper, and simpler methods for nucleic acid analysis in today s clinical tests. Nanotechnologies can potentially provide solutions to these requirements by integrating nanomaterials with biofunctionalities. Dramatic improvement in the sensitivity and multiplexing can be achieved through the high-degree miniaturization. Here, we present our study in the development of an ultrasensitive label-free electronic chip for DNA/RNA analysis based on carbon nanotube nanoelectrode arrays. A reliable nanoelectrode array based on vertically aligned multi-walled carbon nanotubes (MWNTs) embedded in a SiO2 matrix is fabricated using a bottom-up approach. Characteristic nanoelectrode behavior is observed with a low-density MWNT nanoelectrode array in measuring both the bulk and surface immobilized redox species. The open-end of MWNTs are found to present similar properties as graphite edge-plane electrodes, with a wide potential window, flexible chemical functionalities, and good biocompatibility. A BRCA1 related oligonucleotide probe with 18 bases is covalently functionalized at the open ends of the MWNTs and specifically hybridized with an oligonucleotide target as well as a PCR amplicon. The guanine bases in the target molecules are employed as the signal moieties for the electrochemical measurements. Ru(bpy)3(2+) mediator is used to further amplify the guanine oxidation signal. This technique has been employed for direct electrochemical detection of label-free PCR amplicon through specific hybridization with the BRCAl probe. The detection limit is estimated to be less than approximately 1000 DNA molecules, approaching the limit of the sensitivity by laser-based fluorescence techniques in DNA microarray. This system provides a general electronic platform for rapid molecular diagnostics in applications requiring ultrahigh sensitivity, high-degree of miniaturization, simple sample preparation, and low- cost operation.

Li, Jun↗
NASA NTRS2008

Monitoring Delamination of Thermal Barrier Coatings by Near-Infrared and Upconversion Luminescence Imaging

Previous work has demonstrated that TBC delamination can be monitored by incorporating a thin luminescent sublayer that produces greatly increased luminescence intensity from delaminated regions of the TBC. Initial efforts utilized visible-wavelength luminescence from either europium or erbium doped sublayers. This approach exhibited good sensitivity to delamination of electron-beam physical-vapor-deposited (EB-PVD) TBCs, but limited sensitivity to delamination of the more highly scattering plasma-sprayed TBCs due to stronger optical scattering and to interference by luminescence from rare-earth impurities. These difficulties have now been overcome by new strategies employing near-infrared (NIR) and upconversion luminescence imaging. NIR luminescence at 1550 nm was produced in an erbium plus ytterbium co-doped yttria-stabilized zirconia (YSZ) luminescent sublayer using 980-nm excitation. Compared to visible-wavelength luminescence, these NIR emission and excitation wavelengths are much more weakly scattered by the TBC and therefore show much improved depth-probing capabilities. In addition, two-photon upconversion luminescence excitation at 980 nm wavelength produces luminescence emission at 562 nm with near-zero fluorescence background and exceptional contrast for delamination indication. The ability to detect TBC delamination produced by Rockwell indentation and by furnace cycling is demonstrated for both EB-PVD and plasma-sprayed TBCs. The relative strengths of the NIR and upconversion luminescence methods for monitoring TBC delamination are discussed.

Eldridge, J. I.↗
DOE OSTI2022

Recombination between 13 C and 2 H to Form Acetylide ( 13 C 2 2 H – ) Probes Nanoscale Interactions in Lipid Bilayers via Dynamic Secondary Ion Mass Spectrometry: Cholesterol and GM 1 Clustering

Although it is thought that there is lateral heterogeneity of lipid and protein components within biological membranes, probing this heterogeneity has proven challenging. The difficulty in such experiments is due to both the small length scale over which such heterogeneity can occur, and the significant perturbation resulting from fluorescent or spin labeling on the delicate interactions within bilayers. Atomic recombination during dynamic nanoscale secondary ion imaging mass spectrometry (NanoSIMS) is a non-perturbative method for examining nanoscale bilayer interactions. Atomic recombination is a variation on conventional NanoSIMS imaging, whereby an isotope on one molecule combines with a different isotope on another molecule during the ionization process, forming an isotopically enriched polyatomic ion in a distance-dependent manner. In this work, we show that the recombinant ion, 13 C 2 2 H – , is formed in high yield from 13 C- and 2 H-labeled lipids. The low natural abundance of triply labeled acetylide also makes it an ideal ion to probe GM 1 clusters in model membranes and the effects of cholesterol on lipid–lipid interactions. We find evidence supporting the cholesterol condensation effect as well as the presence of nanoscale GM 1 clusters in model membranes.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2025

Characterizing laser-heated polymer foams with simultaneous x-ray fluorescence spectroscopy and Thomson scattering at the Matter in Extreme Conditions Endstation at LCLS

Understanding the behavior of polymer foams at high energy density conditions is crucial to advance inertial fusion energy research. Here, we present a new experimental platform designed to measure the thermodynamic state of these materials at megabar pressures. At the Matter in Extreme Conditions Endstation of the Linac Coherent Light Source, we heat samples using an optical, high-intensity, femtosecond laser and dynamically probe them with ultra-short, coherent x-ray pulses of high peak brightness. We perform x-ray Thomson scattering measurements in forward and backward scattering geometries to capture both collective and non-collective electron behavior in the sample. Simultaneously, x-ray fluorescence spectroscopy is used to measure the emission from a mid-Z dopant, providing complementary information on the plasma conditions. By combining these techniques, we obtain temporally resolved temperature measurements of the transient warm dense matter states. Our initial experiments designed to benchmark the platform with carbon samples yielded data resolving the ultrafast response to laser heating with sub-picosecond resolution, measuring plasma temperatures exceeding 50 eV. These findings lay the foundation for precision studies of the dynamic evolution of laser-heated polymer foams.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
NASA NTRS1998

Detection and quantification of Epstein-Barr virus EBER1 in EBV-infected cells by fluorescent in situ hybridization and flow cytometry

A rapid and highly sensitive fluorescent in situ hybridization (FISH) assay was developed to detect Epstein Barr virus (EBV)-infected cells in peripheral blood. Multiple fluorescein-labeled antisense oligonucleotide probes were designed to hybridize to the EBER1 transcript, which is highly expressed in latently infected cells. After a rapid (30 min) hybridization, the cells were analyzed by flow cytometry. EBER1 was detected in several positive control cell lines that have variable numbers of EBV genome copies. No EBER1 was detected in two known EBV-negative cell lines. Northern blot analyses confirmed the presence and quantity of EBER1 transcripts in each cell line. This method was used to quantify the number of EBV-infected cells in peripheral blood from a patient with chronic mononucleosis. These results indicate that EBV-infected cells can be detected at the single cell level, and that this assay can be used to quantify the number of EBV-infected cells in clinical samples.

NASA Discipline Regulatory Physiology↗
DOE OSTI2021

Characterization of axolotl lampbrush chromosomes by fluorescence in situ hybridization and immunostaining

The lampbrush chromosomes (LBCs) in oocytes of the Mexican axolotl (Ambystoma mexicanum) were identified some time ago by their relative lengths and predicted centromeres, but they have never been associated completely with the mitotic karyotype, linkage maps or genome assembly. We identified 9 of the axolotl LBCs using RNAseq to identify actively transcribed genes and 13 BAC (bacterial artificial clone) probes containing pieces of active genes. Using read coverage analysis to find candidate centromere sequences, we developed a centromere probe that localizes to all 14 centromeres. Measurements of relative LBC arm lengths and polymerase III localization patterns enabled us to identify all LBCs. This study presents a relatively simple and reliable way to identify each axolotl LBC cytologically and to anchor chromosome-length sequences (from the axolotl genome assembly) to the physical LBCs by immunostaining and fluorescence in situ hybridization. Our data will facilitate a more detailed transcription analysis of individual LBC loops.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2021

Complete polarization of electronic spins in OLEDs

At low temperatures and high magnetic fields, electron and hole spins in an organic light-emitting diode become polarized so that recombination preferentially forms molecular triplet excited-state species. For low device currents, magnetoelectroluminescence perfectly follows Boltzmann activation, implying a virtually complete polarization outcome. As the current increases, the magnetoelectroluminescence effect is reduced because spin polarization is suppressed by the reduction in carrier residence time within the device. Under these conditions, an additional field-dependent process affecting the spin-dependent recombination emerges, possibly related to the build-up of triplet excitons and their interaction with free charge carriers. Suppression of the EL alone does not prove electronic spin polarization. We therefore probe changes in the spin statistics of recombination directly in a dual singlet-triplet emitting material, which shows a concomitant rise in phosphorescence intensity as fluorescence is suppressed. Finite spin-orbit coupling in these materials gives rise to a microscopic distribution in effective g-factors of electrons and holes, Δg, i.e., a distribution in Larmor frequencies. This Δg effect in the pair, which mixes singlet and triplet, further suppresses singlet-exciton formation at high fields in addition to thermal spin polarization of the individual carriers.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2025

Spatially resolved measurements of plasma ion velocity distributions in a dipole magnetic field

The equilibrium flows of a plasma discharge in a dipole magnetic field are a topic of interest in low temperature plasma physics. Experimentalists typically rely on probe-based and line-integrated diagnostic techniques in these environments to describe plasma behavior. Presented here are measurements of argon ion dynamics with laser induced fluorescence techniques to provide insight into plasma dynamics in dipole magnetic fields with nonperturbative, spatially localized measurements. Simulation results from a Lagrangian approach to track particle orbits are compared to measured density profiles and provide evidence to support the mechanism distinguishing experimental configurations is the initial approach of particles. Applying a negative DC bias to the magnet induces strong E×B flows around the magnet, even exceeding the ion acoustic speed as measured far from the magnet. A strong enough bias also produces two distinct ion populations and provides a method for controlling the density gradient on the equator.

McLaughlin, Jacob W. (ORCID:0000000152661888)↗
NASA NTRS1991

Automated eddy current inspection of Space Shuttle APU turbine wheel blades

An automated inspection system based on eddy current testing (ET) techniques has been developed to inspect turbine wheel blades on the APU used in NASA's Space Transportation system. The APU is a hydrazine-powered gas turbine with a 15-cm diameter Rene 41 turbine wheel, which has 123 first-stage blades and 123 second-stage blades. The flaw detection capability of the ET system is verified through comparison with fluorescent penetrant test results. Results of the comparison indicate that ET is capable of inspecting surfaces with very restrictive geometries. The ET capability requires development of probes with extremely small coils to allow inspection within 0.4 mm of the blade root and the leading and trailing edges of the blade and within a height restriction of less than 1 mm. The color 2D presentation of the ET data provided crack-growth pattern and length information similar to those found with visual techniques. It also provided visual clues to minimize geometry effects such as generated from blade edges, a neighoring blade, and changes in the blade thickness.

Fisher, Jay L.↗
NASA NTRS1994

Measurement of resistance to solute transport across surfactant-laden interfaces using a Fluorescence Recovery After Photobleaching (FRAP) technique

A noninvasive fluorescence recovery after photobleaching (FRAP) technique is under development to measure interfacial transport in two phase systems without disturbing the interface. The concentration profiles of a probe solute are measured in both sides of the interface by argon-ion laser, and the system relaxation is then monitored by a microscope-mounted CCD camera.

Browne, Edward P.↗
NASA NTRS2003

Physiological Maturation of Regenerating Hair Cells

The bullfrog saccule, a sensor of gravity and substrate-borne vibration, is a model system for hair cell transduction. Saccular hair cells also increase in number throughout adult life and rapidly recover after hair cell damage, making this organ an ideal system for studying hair cell development, repair, and regeneration. We have used of hair cell and supporting cell immunocytochemical markers to identify damaged hair cells and hair cell precursors in organotypic cultures of the bullfrog saccule. We then used an innovative combination of confocal, electron, and time-lapse microscopy to study the fate of damaged hair cells and the origin of new hair cells after gentamicin ototoxicity in normal and mitotically blocked saccular cultures. These studies have shown that gentamicin ototoxicity produces both lethal and sublethal hair cell damage. They have also shown that hair cell recovery in this organ takes place by both the repair of sublethally damaged hair cells and by the replacement of lost hair cells by mitotic regeneration. In parallel studies, we have used biophysical and molecular biological techniques to study the differentiation and innervation of developing, repairing, and regenerating hair cells. More specifically, we have used RT-PCR to obtain the bullfrog homologues of L-type voltage- gated calcium (L-VGCC) and large-conductance Ca(2+)-activated potassium (BK) channel genes. We have then obtained probes for these genes and, using in situ hybridization, begun to examine their expression in the bullfrog saccule and amphibian papilla. We have also used fluorescent-labeled channel toxins and channel toxin derivatives to determine the time of appearance of L-type voltage-gated calcium (L-VGCC) and Ca(2+)-activated potassium (BK) channels and to study dynamic changes in the number, distribution, and co-localization of these proteins in developing, repairing, and regenerating hair cells. Using time-lapse microscopy, we are also studying the dynamic relationship between ion channel clustering and synaptic formation in hair cells and afferent neurons. In future studies, we will determine when hair cell precursors acquire electrical tuning, and, using whole-cell patch-clamp techniques, identify and characterize their L-VGCC and BK currents. We will also use biophysical techniques to determine the number of L-VGCC and BK channels and the size and gating kinetics of their underlying L-VGCC and BK conductances, correlating these variables with the amplitude and frequency of membrane oscillations produced by intracellular current steps. We expect these studies to determine how hair cells regulate ion channel expression to achieve specific physiological responses.

Baird, Richard A.↗