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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 325 records · Page 18

Lateral Gene Transfer Drives Metabolic Flexibility in the Anaerobic Methane-Oxidizing Archaeal Family Methanoperedenaceae

AOM by microorganisms limits the atmospheric release of the potent greenhouse gas methane and has consequent importance for the global carbon cycle and climate change modeling. While the oxidation of methane coupled to sulfate by consortia of anaerobic methanotrophic (ANME) archaea and bacteria is well documented, several other potential electron acceptors have also been reported to support AOM. In this study, we identify a number of novel respiratory strategies that appear to have been laterally acquired by members of the Methanoperedenaceae , as they are absent from related archaea and other ANME lineages. Expanding the known metabolic potential for members of the Methanoperedenaceae provides important insight into their ecology and suggests their role in linking methane oxidation to several global biogeochemical cycles.

59 BASIC BIOLOGICAL SCIENCES↗

gismo-cloud-deployment

Tools for executing time-consuming tasks with developer-defined custom code blocks in parallel on the AWS EKS platform.

Leu, Jimmy↗

An African-Specific Variant of TP5 3 Reveals PADI4 as a Regulator of p53-Mediated Tumor Suppression

TP53 is the most frequently mutated gene in cancer, yet key target genes for p53-mediated tumor suppression remain unidentified. Here, we characterize a rare, African-specific germline variant of TP53 in the DNA-binding domain Tyr107His (Y107H). Nuclear magnetic resonance and crystal structures reveal that Y107H is structurally similar to wild-type p53. Consistent with this, we find that Y107H can suppress tumor colony formation and is impaired for the transactivation of only a small subset of p53 target genes; this includes the epigenetic modifier PADI4, which deiminates arginine to the nonnatural amino acid citrulline. Surprisingly, we show that Y107H mice develop spontaneous cancers and metastases and that Y107H shows impaired tumor suppression in two other models. We show that PADI4 is itself tumor suppressive and that it requires an intact immune system for tumor suppression. We identify a p53–PADI4 gene signature that is predictive of survival and the efficacy of immune-checkpoint inhibitors.

60 APPLIED LIFE SCIENCES↗

Expanded Validation of Uranium Systems with the KENO Monte Carlo Codes and SCALE 6.2.4

The development of accident-tolerant fuel and advanced reactors fueled with high-assay low-enriched uranium has created an increased focus on systems with uranium enrichments between 5 and 20 wt % 235 U. SCALE criticality safety validation reports generated at Oak Ridge National Laboratory contain very few benchmark experiments in this range. The work presented herein is an initial investigation of expanded validation of the entire range of enrichments for uranium-fueled systems, including consideration of over 300 newly developed KENO models. The vast majority of the new models are between 4.4 wt % and 37.5 wt % enrichment and include a variety of thermal, intermediate, and mixed neutron spectra. This work finds no strong evidence of an enrichment-dependent bias, but there are indications of a spectrum-dependent bias. There are, however, only a limited number of independent experiments included at this time to distinguish intermediate enrichment effects from intermediate spectrum effects. Further work in this area is ongoing for intermediate enrichment systems. In the long run, the addition of these models to the Verified, Archived Library and Inputs and Data is planned, as funding and staff availability allow.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Substrate-embedded metal meshes for ITO-free organic light emitting diodes

Organic light-emitting diodes (OLEDs) have great potential for use in large-area display and lighting applications, but their widespread adoption for large areas is hindered by the high cost and insufficient performance of indium tin oxide (ITO) anodes. In this study, we introduce an alternative anode material – a silver mesh embedded in glass – to facilitate production of large-area OLEDs. We present a facile, scalable manufacturing technique to create high aspect ratio micromeshes embedded in glass to provide the planar geometry needed for OLED layers. Our phosphorescent green OLEDs achieve a current efficiency of 51.4 cd/A at 1000 cd/m 2 and reach a slightly higher external quantum efficiency compared to a standard ITO/glass reference sample. Notably, these advancements are achieved without any impact on the viewing angle of the OLEDs. These findings represent a promising advancement towards ITO-free, high-efficiency OLEDs for various high performance, large-area applications, such as lighting and displays.

Optics↗

Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea

The anaerobic oxidation of methane coupled to sulfate reduction is a microbially mediated process requiring a syntrophic partnership between anaerobic methanotrophic (ANME) archaea and sulfate-reducing bacteria (SRB). Based on genome taxonomy, ANME lineages are polyphyletic within the phylum Halobacterota , none of which have been isolated in pure culture. Here, we reconstruct 28 ANME genomes from environmental metagenomes and flow sorted syntrophic consortia. Together with a reanalysis of previously published datasets, these genomes enable a comparative analysis of all marine ANME clades. We review the genomic features that separate ANME from their methanogenic relatives and identify what differentiates ANME clades. Large multiheme cytochromes and bioenergetic complexes predicted to be involved in novel electron bifurcation reactions are well distributed and conserved in the ANME archaea, while significant variations in the anabolic C1 pathways exists between clades. Our analysis raises the possibility that methylotrophic methanogenesis may have evolved from a methanotrophic ancestor.

59 BASIC BIOLOGICAL SCIENCES↗

PNNL's Characterization Summary for MP-2 Experiment

Characterization of as-fabricated fuel was performed at Pacific Northwest National Laboratory (PNNL) in accordance with the characterization plan for the fabrication of U 10Mo plate fuel for the U.S. High Performance Research Reactor conversion program’s Fuel Fabrication Pillar (INL 2021). Similar characterization work is also being performed at Idaho National Laboratory to provide a detailed understanding of the as-fabricated foils that would be irradiated in the Mini-Plate 2 (MP 2) experiment. Under the MP 2 characterization plan, foils are studied that have different fabrication parameters (such as rolling condition, rolling thickness reduction, co-rolling with Zr layers). Similar samples from master foils were sent to both the organizations, so that the testing and analysis can be done independently using similar equipment and standardized measurement and analysis procedures. A final, consolidated report will be prepared based on this work and will summarize all the information obtained from the two laboratories. The MP 2 experiment will provide an opportunity to understand the effects of processing conditions on the final fuel microstructure, to compare results obtained independently, and achieve a two-way validation. In Fiscal Year 2022, PNNL received five MP 2 cast (PD STD2) samples to examine the foils’ chemistry and microstructure. For each cast sample, PNNL received samples from three different locations. PNNL also received and characterized 24 U 10Mo foil samples, by sectioning four pieces/specimens from each foil, in accordance with the MP 2 Characterization Plan (INL 2021). These 24 samples consist of four types of foils from BWX Technologies: 0.047 in. thick hot-rolled and annealed samples with Zr layers; 0.025 in. thick cold-rolled and annealed samples with Zr layers; 0.0105 in. thick cold-rolled and annealed samples with Zr layers. Along with these, PNNL also received four plates with Zr layers that were 0.025 in. and 0.0105 in. thick. This report describes the results of PNNL’s MP 2 foil characterization. Microstructure, Mo homogeneity, carbide fraction and morphology, U 10Mo foil thickness, and Zr thickness were evaluated in both the longitudinal and transverse directions for all the foils of the three different thicknesses.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Fuel Fabrication Capability Assessment in Support of Advanced Reactor Deployments

More than 30 U.S. companies are designing a variety of advanced reactor concepts, and several companies are planning to demonstrate their reactor designs in the mid-2020s to late 2030s time frame. In 2020, the U.S. Department of Energy (DOE) announced a series of awards under the Advanced Reactor Demonstration Program (ARDP) to accelerate the successful deployment of 10 of these reactors under three pathways. TerraPower and X-energy were awarded grants under the Advanced Reactor Demonstration Program to deploy their respective Natrium reactor and Xe-100 reactor designs in the next 7–10 years. These demonstrations are in addition to several parallel programs, including the U.S. Department of Defense’s (DoD’s) interest in the development of microreactors, and interest of the National Aeronautics and Space Administration in space nuclear power and propulsion. The National Reactor Innovation Center’s (NRIC’s) mission is to accelerate the demonstration and deployment of advanced reactors; NRIC is partnering with several reactor developers and harnessing the world-class capabilities of the U.S. National Laboratory system to deliver on its mission. Several of these reactor designs will require advanced fuel forms that are not commercially available today, including metal fuel, molten salt fuel, TRi-structural ISOtropic (TRISO) particle fuel, and uranium nitride fuel. Recognizing that there may be potential gaps in the laboratory-scale process development and pilot-scale first-of-a-kind (FOAK) production of these fuel forms leading to delivery of the FOAK cores, NRIC commissioned this study to look at the challenges that need to be overcome for successful deliveries, including the evaluation of existing facilities and the potential need for a new fuel fabrication facility.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

A Review of Candidates for a Validation Data Set for High-Assay Low-Enrichment Uranium Fuels

Many advanced reactor concept designs rely on high-assay low-enriched uranium (HALEU) fuel, enriched up to approximately 19.75% 235 U by weight. Efforts are underway by the US government to increase HALEU production in the United States to meet anticipated needs. However, very few data exist for validation of computational models that include HALEU, beyond a few fresh fuel benchmark specifications in the International Reactor Physics Experiment Evaluation Project. Nevertheless, there are other data with potential value available for developing into quality benchmarks for use in data- and software-validation efforts. This paper reviews the available evaluated HALEU fuel benchmarks and some of the potentially relevant benchmarks for fresh highly enriched uranium. It then introduces experimental data for HALEU fuel irradiated at Idaho National Laboratory, from relatively recent irradiation programs at the Advanced Test Reactor. Such data should be evaluated and, if valuable, collected into detailed benchmark specifications to meet the needs of HALEU-based reactor designers.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

MSFN transmitters

Implementation and testing of Deep Space Network Manned Space Flight Network transmitters

DEEP SPACE INSTRUMENTATION FACILITY /DSIF/↗

Measurements of recombination of electrons with H3O(plus)-(H2O)n series ions

Recombination coefficients of electrons with ions of the hydrated hydronium ion series (H3O(+))(H2O)n, where n = 0, 1, 2, 3, 4, 5, 6, were determined with a microwave afterglow/mass spectrometer apparatus. Afterglow measurements of electron density decays in helium-water vapor mixtures are correlated with the decay of mass-identified ion currents to the wall of the microwave cavity. By varying the temperature of the gas and the partial pressure of the water vapor in the mixture, different groups of hydronium series ions are made to dominate the afterglow.

Leu, M. T.↗

Measurements of recombination of electrons with HCO(plus) ions

Recombination coefficients of electrons with HCO(+) ions were determined with a microwave afterglow/mass spectrometer apparatus. Afterglow measurements of electron density decays in neon-hydrogen-carbon monoxide mixtures are correlated with the decay of mass-identified ion currents to the wall of the microwave cavity. At the appropriate partial pressures of hydrogen and carbon monoxide in the mixture, the ion HCO(+) dominates the ion composition and its wall current approximately tracks the electron density decay curve. From recombination controlled electron density decay curves, the values alpha (HCO(+)) = (3.3 + or - 0.5) and (2.0 + or - 0.3) 0.0000001 cu cm/sec are obtained at 205 and 300 K, respectively. The implications of these results for models of polyatomic molecule formation in dense interstellar clouds are briefly discussed.

Leu, M. T.↗

Measurements of recombination of electrons with H3(plus) and H5(plus) ions

The electron-ion recombination coefficients for H3(+) and H5(+) ions were determined by means of a microwave afterglow/mass spectrometer apparatus. Measurements of electron density decays in helium-hydrogen mixtures are correlated with the decay of mass-identified ion currents to the wall of the microwave cavity. At low partial pressures of hydrogen in the mixture, the ion H3(+) dominates the ion composition and the ion wall current tracks the electron density decay curves. From recombination controlled electron density decay curves, the values alpha (H3(+)) = (2.9 + or - 0.3), (2.3 + or - 0.3), and (2.0 + or - 0.2) x 0.0000001 cu cm per sec, are obtained at 205, 300 and 450 K, respectively. At higher partial pressures of hydrogen and low temperatures, where (H5(+)) is the dominant ion, the value alpha (H5(+)) = (3.6 + or - 1.0) x 0.0000001 cu cm per sec is obtained at 205 K. The implications of these results concerning ionization levels in the atmospheres of the outer planets and in the interstellar medium are discussed.

Leu, M. T.↗

Transmitter switch for high-power microwave output

Combiner system can be used for combining output powers of two transmitters or for switching from one to the other. This can be done when pair of transmitters operate on same frequency and carriers are phase coherent as by excitation from single exciter.

Wiggins, C. P.↗

Analysis of three-component aeromagnetic data

The numerical method of obtaining three field components from total field measurements, using double Fourier series expansion, is presented. The expressions for moments of the anomalous field components over a finite area are given. The magnitude and direction of the magnetization vector indicate that the vertical component of the magnetic field calculated from total field observations is more accurate at higher geomagnetic latitudes than at lower latitudes. The opposite is true for the horizontal components. The error in determining the magnetization vector directions are significantly large over most of the range of variation of declination and inclination of the vector, demonstrating the practical limitations of computing field components from total field data even under the best of conditions.

Bhattacharyya, B. K.↗