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NASA NTRS1999

Quantitative Method of Measuring Metastatic Activity

The metastatic potential of tumors can be evaluated by the quantitative detection of urokinase and DNA. The cell sample selected for examination is analyzed for the presence of high levels of urokinase and abnormal DNA using analytical flow cytometry and digital image analysis. Other factors such as membrane associated uroldnase, increased DNA synthesis rates and certain receptors can be used in the method for detection of potentially invasive tumors.

Morrison, Dennis R.↗

NASA NTRS1999

A Compact, Tunable Near-UV Source for Quantitative Microgravity Combustion Diagnostics

There is a need for improved optical diagnostic methods for use in microgravity combustion research. Spectroscopic methods with fast time response that can provide absolute concentrations and concentration profiles of important chemical species in flames are needed to facilitate the understanding of combustion kinetics in microgravity. Although a variety of sophisticated laser-based diagnostics (such as planar laser induced fluorescence, degenerate four wave mixing and coherent Raman methods) have been applied to the study of combustion in laboratory flames, the instrumentation associated with these methods is not well suited to microgravity drop tower or space station platforms. Important attributes of diagnostic systems for such applications include compact size, low power consumption, ruggedness, and reliability. We describe a diode laser-based near-UV source designed with the constraints of microgravity research in mind. Coherent light near 420 nm is generated by frequency doubling in a nonlinear crystal. This light source is single mode with a very narrow bandwidth suitable for gas phase diagnostics, can be tuned over several 1/cm and can be wavelength modulated at up to MHz frequencies. We demonstrate the usefulness of this source for combustion diagnostics by measuring CH radical concentration profiles in an atmospheric pressure laboratory flame. The radical concentrations are measured using wavelength modulation spectroscopy (WMS) to obtain the line-of-sight integrated absorption for different paths through the flame. Laser induced fluorescence (LIF) measurements are also demonstrated with this instrument, showing the feasibility of simultaneous WMS absorption and LIF measurements with the same light source. LIF detection perpendicular to the laser beam can be used to map relative species densities along the line-of-sight while the integrated absorption available through WMS provides a mathematical constraint on the extraction of quantitative information from the LIF data. Combining absorption with LIF - especially if the measurements are made simultaneously with the same excitation beam - may allow elimination of geometrical factors and effects of intensity fluctuations (common difficulties with the analysis of LIF data) from the analysis.

Peterson, K. A.↗

NASA NTRS1998

An Overview of Quantitative Risk Assessment of Space Shuttle Propulsion Elements

Since the Space Shuttle Challenger accident in 1986, NASA has been working to incorporate quantitative risk assessment (QRA) in decisions concerning the Space Shuttle and other NASA projects. One current major NASA QRA study is the creation of a risk model for the overall Space Shuttle system. The model is intended to provide a tool to estimate Space Shuttle risk and to perform sensitivity analyses/trade studies, including the evaluation of upgrades. Marshall Space Flight Center (MSFC) is a part of the NASA team conducting the QRA study; MSFC responsibility involves modeling the propulsion elements of the Space Shuttle, namely: the External Tank (ET), the Solid Rocket Booster (SRB), the Reusable Solid Rocket Motor (RSRM), and the Space Shuttle Main Engine (SSME). This paper discusses the approach that MSFC has used to model its Space Shuttle elements, including insights obtained from this experience in modeling large scale, highly complex systems with a varying availability of success/failure data. Insights, which are applicable to any QRA study, pertain to organizing the modeling effort, obtaining customer buy-in, preparing documentation, and using varied modeling methods and data sources. Also provided is an overall evaluation of the study results, including the strengths and the limitations of the MSFC QRA approach and of qRA technology in general.

Safie, Fayssal M.↗

NASA NTRS1999

The Upper Atmosphere Research Satellite: From Coffee Table Art to Quantitative Science

The Upper Atmosphere Research Satellite (UARS) has provided an unprecedented set of observations of constituents of the stratosphere. When used in combination with data from other sources and appropriate modeling tools, these observations are useful for quantitative evaluation of stratospheric photochemical processes. This is illustrated by comparing ozone observations from airborne Differential Absorption Lidar (DIAL), from the Polar Ozone and Aerosol Measurement (POAM), from the Microwave Limb Sounder (MLS), and from the Halogen occultation Experiment (HALOE) with ozone fields generated with a three dimensional model. For 1995-96, at polar latitudes, observations from DIAL flights on December 9 and January 30, and POAM and MLS between late December and late January are compared with ozone fields from the GSFC 3D chemistry and transport model. Data from the three platforms consistently show that the observed ozone has a negative trend relative to the modeled ozone, and that the trend is uniform in time between early and mid winter, with no obvious dependence on proximity to the vortex edge. The importance of chlorine catalyzed photochemistry to this ozone loss is explored by comparing observations from MLS and HALOE with simulations for other northern winters, particularly 1997-98.

Douglass, Anne R.↗

NASA NTRS1999

Impact of Model Uncertainties on Quantitative Analysis of FUV Auroral Images: Peak Production Height

We demonstrate that small uncertainties in the modeled height of peak production for FUV emissions can lead to significant uncertainties in the analysis of these sai-ne emissions. In particular, an uncertainty of only 3 km in the peak production height can lead to a 50% uncertainty in the mean auroral energy deduced from the images. This altitude uncertainty is comparable to differences in different auroral deposition models currently used for UVI analysis. Consequently, great care must be taken in quantitative photometric analysis and interpretation of FUV auroral images.

Germany, G. A.↗

NASA NTRS1999

Erosion of Terrestrial Rift Flank Topography: A Quantitative Study

Many rifted or passive continental margins feature a seaward-facing erosional escarpment which abruptly demarcates deeply weathered, low relief, interior uplands from a deeply incised, high relief coastal zone. It is generally accepted that these escarpments originate at the time of continental rifting and propagate inland through the elevated rift flank topography at rates on the order of 1 km/Myr over the course of a margin's history. Considering the length of passive margins worldwide and an average rift flank plateau height of several hundred meters, it is clear that sediment eroded from passive margins is an important component of the mass flux from continents to oceans through geologic time. The overall goal of the research reported here is to develop a quantitative understanding of the kinematics of escarpment propagation across passive margins and the underlying geological processes responsible for this behavior. Plateau-bounding escarpments in general exhibit two basic forms depending on the direction of surface water drainage on the plateau interior relative to the escarpment. Where surface water flows away from the escarpment, the escarpment takes the form of subdued embayments and promontories, such that its overall trend remains fairly straight as it evolves with time. Where upland streams flow across the escarpment, it takes the form of dramatic, narrow gorges whose heads appear to propagate up the plateau drainage systems as large-scale knickpoints. From work on the Colorado Plateau, Schmidt (1987) noted that the Colorado River is located much closer to the Grand Canyon's south rim, a drainage divide escarpment, than to the north rim, which is a gorge-like escarpment. The main implication is that the gorge-like form might be associated with higher long-term average erosion rates compared to the drainage divide escarpment type.

Weissel, Jeffrey K.↗

NASA NTRS1999

Quantitative Velocity Field Measurements in Reduced-Gravity Combustion Science and Fluid Physics Experiments

Systems have been developed and demonstrated for performing quantitative velocity measurements in reduced gravity combustion science and fluid physics investigations. The unique constraints and operational environments inherent to reduced-gravity experimental facilities pose special challenges to the development of hardware and software systems. Both point and planar velocimetric capabilities are described, with particular attention being given to the development of systems to support the International Space Station laboratory. Emphasis has been placed on optical methods, primarily arising from the sensitivity of the phenomena of interest to intrusive probes. Limitations on available power, volume, data storage, and attendant expertise have motivated the use of solid-state sources and detectors, as well as efficient analysis capabilities emphasizing interactive data display and parameter control.

Greenberg, Paul S.↗

NASA NTRS1999

Quantitative Interferometry in the Severe Acoustic Environment of Resonant Supersonic Jets

Understanding fundamental fluidic dynamic and acoustic processes in high-speed jets requires quantitative velocity, density and temperature measurements. In this paper we demonstrate a new, robust Liquid Crystal Point Diffraction Interferometer (LCPDI) that includes phase stepping and can provide accurate data even in the presence of intense acoustic fields. This novel common path interferometer (LCPDI) was developed to overcome difficulties with the Mach Zehnder interferometer in vibratory environments and is applied here to the case of a supersonic shock- containing jet. The environmentally insensitive LCPDI that is easy to align and capable of measuring optical wavefronts with high accuracy is briefly described, then integrated line of sight density data from the LCPDI for two underexpanded jets are presented.

Mercer, Carolyn R.↗

NASA NTRS1999

Quantitative EEG Monitoring of Vigilance: Effects of Sleep Deprivation, Circadian Phase and Sympathetic Activation

Shuttle astronauts typically sleep only 6 to 6.5 hours per day while in orbit. This sleep loss is related to recurrent sleep cycle shifting--due to mission-dependent orbital mechanics and mission duration requirements-- and associated circadian displacement of sleep, the operational demands of space flight, noise and space motion sickness. Such sleep schedules are known to produce poor subjective sleep quality, daytime sleepiness, reduced attention, negative mood, slower reaction times, and impaired daytime alertness. Countermeasures to allow crew members to obtain an adequate amount of sleep and maintain adequate levels of neurobehavioral performance are being developed and investigated. However, it is necessary to develop methods that allow effective and attainable in-flight monitoring of vigilance to evaluate the effectiveness of these countermeasures and to detect and predict online critical decrements in alertness/performance. There is growing evidence to indicate that sleep loss and associated decrements in neurobehavioral function are reflected in the spectral composition of the electroencephalogram (EEG) during wakefulness as well as in the incidence of slow eye movements recorded by the electro-oculogram (EOG). Further-more, our preliminary data indicated that these changes in the EEG during wakefulness are more pronounced when subjects are in a supine posture, which mimics some of the physiologic effects of microgravity. Therefore, we evaluate the following hypotheses: (1) that during a 40-hour period of wakefulness (i.e., one night of total sleep deprivation) neurobehavioral function deteriorates, the incidence of slow eye-movements and EEG power density in the theta frequencies increases especially in frontal areas of the brain; (2) that the sleep deprivation induced deterioration of neurobehavioral function and changes in the incidence of slow eye movements and the spectral composition of the EEG are more pronounced when subjects are in a supine position; and (3) that based on assessment of slow-eye movements and quantitative on-line topographical analyses of EEG during wakefulness an EEG and or EOG parameter can be derived/constructed which accurately predicts changes in neurobehavioral function.

Dijk, Derk-Jan↗

NASA NTRS2000

COTS-Based Fault Tolerance in Deep Space: Qualitative and Quantitative Analyses of a Bus Network Architecture

Using COTS products, standards and intellectual properties (IPs) for all the system and component interfaces is a crucial step toward significant reduction of both system cost and development cost as the COTS interfaces enable other COTS products and IPs to be readily accommodated by the target system architecture. With respect to the long-term survivable systems for deep-space missions, the major challenge for us is, under stringent power and mass constraints, to achieve ultra-high reliability of the system comprising COTS products and standards that are not developed for mission-critical applications. The spirit of our solution is to exploit the pertinent standard features of a COTS product to circumvent its shortcomings, though these standard features may not be originally designed for highly reliable systems. In this paper, we discuss our experiences and findings on the design of an IEEE 1394 compliant fault-tolerant COTS-based bus architecture. We first derive and qualitatively analyze a -'stacktree topology" that not only complies with IEEE 1394 but also enables the implementation of a fault-tolerant bus architecture without node redundancy. We then present a quantitative evaluation that demonstrates significant reliability improvement from the COTS-based fault tolerance.

Tai, Ann T.↗

NASA NTRS1997

Quantitative Laser-Saturated Fluorescence Measurements of Nitric Oxide in a Heptane Spray Flame

We report spatially resolved laser-saturated fluorescence measurements of NO concentration in a pre-heated, lean-direct injection (LDI) spray flame at atmospheric pressure. The spray is produced by a hollow-cone, pressure-atomized nozzle supplied with liquid heptane. NO is excited via the Q2(26.5) transition of the gamma(0,0) band. Detection is performed in a 2-nm region centered on the gamma(0,1) band. Because of the relatively close spectral spacing between the excitation (226 nm) and detection wavelengths (236 nm), the gamma(0,1) band of NO cannot be isolated from the spectral wings of the Mie scattering signal produced by the spray. To account for the resulting superposition of the fluorescence and scattering signals, a background subtraction method has been developed that utilizes a nearby non-resonant wavelength. Excitation scans have been performed to locate the optimum off-line wavelength. Detection scans have been performed at problematic locations in the flame to determine possible fluorescence interferences from UHCs and PAHs at both the on-line and off-line excitation wavelengths. Quantitative radial NO profiles are presented and analyzed so as to better understand the operation of lean-direct injectors for gas turbine combustors.

Cooper, Clayton S.↗

NASA NTRS2001

Quantitative Surface Emissivity and Temperature Measurements of a Burning Solid Fuel Accompanied by Soot Formation

Surface radiometry is an established technique for noncontact temperature measurement of solids. We adapt this technique to the study of solid surface combustion where the solid fuel undergoes physical and chemical changes as pyrolysis proceeds, and additionally may produce soot. The physical and chemical changes alter the fuel surface emissivity, and soot contributes to the infrared signature in the same spectral band as the signal of interest. We have developed a measurement that isolates the fuel's surface emissions in the presence of soot, and determine the surface emissivity as a function of temperature. A commercially available infrared camera images the two-dimensional surface of ashless filter paper burning in concurrent flow. The camera is sensitive in the 2 to 5 gm band, but spectrally filtered to reduce the interference from hot gas phase combustion products. Results show a strong functional dependence of emissivity on temperature, attributed to the combined effects of thermal and oxidative processes. Using the measured emissivity, radiance measurements from several burning samples were corrected for the presence of soot and for changes in emissivity, to yield quantitative surface temperature measurements. Ultimately the results will be used to develop a full-field, non-contact temperature measurement that will be used in spacebased combustion investigations.

Piltch, Nancy D.↗

NASA NTRS1997

Quantitative Simulations of MST Visual Receptive Field Properties Using a Template Model of Heading Estimation

We previously developed a template model of primate visual self-motion processing that proposes a specific set of projections from MT-like local motion sensors onto output units to estimate heading and relative depth from optic flow. At the time, we showed that that the model output units have emergent properties similar to those of MSTd neurons, although there was little physiological evidence to test the model more directly. We have now systematically examined the properties of the model using stimulus paradigms used by others in recent single-unit studies of MST: 1) 2-D bell-shaped heading tuning. Most MSTd neurons and model output units show bell-shaped heading tuning. Furthermore, we found that most model output units and the finely-sampled example neuron in the Duffy-Wurtz study are well fit by a 2D gaussian (sigma approx. 35deg, r approx. 0.9). The bandwidth of model and real units can explain why Lappe et al. found apparent sigmoidal tuning using a restricted range of stimuli (+/-40deg). 2) Spiral Tuning and Invariance. Graziano et al. found that many MST neurons appear tuned to a specific combination of rotation and expansion (spiral flow) and that this tuning changes little for approx. 10deg shifts in stimulus placement. Simulations of model output units under the same conditions quantitatively replicate this result. We conclude that a template architecture may underlie MT inputs to MST.

Stone, Leland S.↗

NASA NTRS1998

Making Intercontinental Burning Influences Quantitative: Lessons on Transport and Geochemistry From the 3-D Simulation of CO in PEM-T A and TRACE-A

We report on two aspects of the simulation of global transport of plumes originating from subtropical biomass fires. We use of meteorological assimilation (MM5) at 2-degree resolution with a Grail cloud parameterization and a Blackadar-based planetary boundary layer parameterization. Ames's GRACES model provides emissions, transport, and an appropriate level of simulated chemical transformation. We have worked with passive-tracer CO or linear chemistry. This is appropriate since we find major work to be done in evaluating CO source strengths and transport mechanisms before chemical integrations could be meaningful. First, we present mechanisms by which CO and other pollutants are introduced into the free troposphere, and are then transported with hate dilution from approx. 0 to approx. 180 degrees longitude. One principal conduit for these plumes is the vernal subtropical jet; however the plumes appear at various altitudes and latitudes as they influenced by frontal motions and (most likely) radiative processes. A common, repeated pattern of transport has pollutant plumes arriving in the distant Pacific Ocean from Africa and South America at 25 degrees south and 14 km altitude. Following this, there is then a general appearance of pollution at extending down to 5 kin at more equatorial (10 S) and polar latitudes (to 45 S). Second, we evaluate the quantitative success of our simulation. (Such success requires efforts considerably beyond trajectory analyses, and is necessary for our community to claim an understanding of the effects of biomass burning on global atmospheric chemistry and the planet's trend in oxidizing capacity.) We find that we simulate most pollution episodes sampled by Glenn Sachse's CO instrument and the Blake hydrocarbon analyses during PENT A. We will present our current ideas on why our general levels appear satisfactory when the observations are within 20 ppb of background levels, but substantially miss the variability associated with the most intense biomass burning plumes. As time allows, we will speculate on the origins of a class of plumes that appear in the mid-troposphere, but are missing from our simulations.

Chatfield, Robert↗

NASA NTRS2003

Quantitative Studies on the Propagation and Extinction of Near-Limit Premixed Flames Under Normal and Microgravity

The main objective of this research is to introduce accurate fluid mechanics measurements diagnostics in the 2.2-s drop tower for the determination of the detailed flow-field at the states of extinction. These results are important as they can then be compared with confidence with detailed numerical simulations so that important insight is provided into near-limit phenomena that are controlled by not well-understood kinetics and thermal radiation processes. Past qualitative studies did enhance our general understanding on the subject. However, quantitative studies are essential for the validation of existing models that subsequently be used to describe near-limit phenomena that can initiate catastrophic events in micro- and/or reduced gravity environments.

Dong, Y.↗

NASA NTRS2004

An Evaluation of Quantitative Methods of Determining the Degree of Melting Experienced by a Chondrule

Many workers have considered the degree to which partial melting occurred in chondrules they have studied, and this has led to attempts to find reliable methods of determining the degree of melting. At least two quantitative methods have been used in the literature: a convolution index (CVI), which is a ratio of the perimeter of the chondrule as seen in thin section divided by the perimeter of a circle with the same area as the chondrule, and nominal grain size (NGS), which is the inverse square root of the number density of olivines and pyroxenes in a chondrule (again, as seen in thin section). We have evaluated both nominal grain size and convolution index as melting indicators. Nominal grain size was measured on the results of a set of dynamic crystallization experiments previously described, where aliquots of LEW97008(L3.4) were heated to peak temperatures of 1250, 1350, 1370, and 1450 C, representing varying degrees of partial melting of the starting material. Nominal grain size numbers should correlate with peak temperature (and therefore degree of partial melting) if it is a good melting indicator. The convolution index is not directly testable with these experiments because the experiments do not actually create chondrules (and therefore they have no outline on which to measure a CVI). Thus we had no means to directly test how well the CVI predicted different degrees of melting. Therefore, we discuss the use of the CVI measurement and support the discussion with X-ray Computed Tomography (CT) data.

Nettles, J. W.↗

NASA NTRS2004

Quantitative Rainbow Schlieren Deflectometry as a Temperature Diagnostic for Spherical Flames

Numerical analysis and experimental results are presented to define a method for quantitatively measuring the temperature distribution of a spherical diffusion flame using Rainbow Schlieren Deflectometry in microgravity. First, a numerical analysis is completed to show the method can suitably determine temperature in the presence of spatially varying species composition. Also, a numerical forward-backward inversion calculation is presented to illustrate the types of calculations and deflections to be encountered. Lastly, a normal gravity demonstration of temperature measurement in an axisymmetric laminar, diffusion flame using Rainbow Schlieren deflectometry is presented. The method employed in this paper illustrates the necessary steps for the preliminary design of a Schlieren system. The largest deflections for the normal gravity flame considered in this paper are 7.4 x 10(-4) radians which can be accurately measured with 2 meter focal length collimating and decollimating optics. The experimental uncertainty of deflection is less than 5 x 10(-5) radians.

Feikema, Douglas A.↗

NASA NTRS2004

Quantitative Prediction of Computational Quality (so the S and C Folks will Accept it)

Our choice of title may seem strange but we mean each word. In this talk, we are not going to be concerned with computations made "after the fact", i.e. those for which data are available and which are being conducted for explanation and insight. Here we are interested in preventing S&C design problems by finding them through computation before data are available. For such a computation to have any credibility with those who absorb the risk, it is necessary to quantitatively PREDICT the quality of the computational results.

Hemsch, Michael J.↗