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At least 325 records · Page 18

Structural Basis for "Flip-Flop" Action of Human Pyruvate Dehydrogenase

The derivative of vitamin B1, thiamin pyrophosphate is a cofactor of pyruvate dehydrogenase, a component enzyme of the mitochondrial pyruvate dehydrogenase multienzyme complex that plays a major role in directing energy metabolism in the cell. This cofactor is used to cleave the C(sup alpha)-C(=O) bond of pyruvate followed by reductive acetyl transfer to lipoyl-dihydrolipoamide acetyltransferase. In alpha(sub 2)beta(sub 2)-tetrameric human pyruvate dehydrogenase, there are two cofactor binding sites, each of them being a center of independently conducted, although highly coordinated enzymatic reactions. The dynamic nonequivalence of two, otherwise chemically equivalent, catalytic sites can now be understood based on the recently determined crystal structure of the holo-form of human pyruvate dehydrogenase at 1.95A resolution. The structure of pyruvate dehydrogenase was determined using a combination of MAD phasing and molecular replacement followed by rounds of torsion-angles molecular-dynamics simulated-annealing refinement. The final pyruvate dehydrogenase structure included coordinates for all protein amino acids two cofactor molecules, two magnesium and two potassium ions, and 742 water molecules. The structure was refined to R = 0.202 and R(sub free) = 0.244. Our structural analysis of the enzyme folding and domain assembly identified a simple mechanism of this protein motion required for the conduct of catalytic action.

Ciszak, Ewa

A High-Order Method Using Unstructured Grids for the Aeroacoustic Analysis of Realistic Aircraft Configurations

A method for the prediction of acoustic scatter from complex geometries is presented. The discontinuous Galerkin method provides a framework for the development of a high-order method using unstructured grids. The method's compact form contributes to its accuracy and efficiency, and makes the method well suited for distributed memory parallel computing platforms. Mesh refinement studies are presented to validate the expected convergence properties of the method, and to establish the absolute levels of a error one can expect at a given level of resolution. For a two-dimensional shear layer instability wave and for three-dimensional wave propagation, the method is demonstrated to be insensitive to mesh smoothness. Simulations of scatter from a two-dimensional slat configuration and a three-dimensional blended-wing-body demonstrate the capability of the method to efficiently treat realistic geometries.

Atkins, Harold L.

Refinement of Transition-Edge Sensor Dimensions for the X-ray Integral Field Unit on ATHENA

At NASA Goddard Space Flight Center, we have previously demonstrated a kilo-pixel array of transition-edge sensor (TES) microcalorimeters capable of meeting the energy resolution requirements of the future X-ray Integral Field Unit (X-IFU) instrument that is being developed for the Advanced Telescope for High ENergy Astrophysics (ATHENA) observatory satellite. The TES design in this array was a square device with side length of 50 μm. Here, we describe studies of TES designs with small variations of the dimensions, exploring lengths, parallel to the current direction, ranging from 75μm to 50μm and widths, perpendicular to the current direction, ranging from 50μm to 15 μm. We describe how these changes impact transition properties, thermal conductance and magnetic field sensitivity. In particular, we show that using a TES with a length of 50μm and width of 30μm may be a promising route to reduce the maximum time-derivative of the TES current in an X-ray pulse and reduce the sensitivity of the TES to magnetic field.

N A Wakeham

The CHARIS High-Contrast Integral-Field Spectrograph

One of the leading direct Imaging techniques, particularly in ground-based imaging, uses a coronagraphic system and integral field spectrograph (IFS). The Coronagraphic High Angular Resolution Imaging Spectrograph (CHARIS) is an IFS that has been built for the Subaru telescope. CHARIS has been delivered to the observatory and now sits behind the Subaru Coronagraphic Extreme Adaptive Optics (SCExAO) system. CHARIS has 'high' and 'low' resolution operating modes. The "high-resolution" mode is used to characterize targets in J, H, and K bands at ~R70. The "low-resolution" prism is meant for discovery and spans J+H+K bands (1.15-2.37 microns) with a spectral resolution of ~R18. This discovery mode has already proven better than 15-sigma detections of HR8799c,d,e when combining ADI+SDI. Using SDI alone, planets c and d have been detected in a single 24 second image. The CHARIS team is optimizing instrument performance and refining ADI+SDI recombination to maximize our contrast detection limit. In addition to the new observing modes, CHARIS has demonstrated a design with high robustness to spectral crosstalk. CHARIS is in the final stages of commissioning, with the instrument open for science observations beginning February 2017. Here we review the science case, design, on-sky performance, engineering observations of exoplanet and disk targets, and specific lessons learned for extremely high contrast imagers. Key design aspects that will be demonstrated are crosstalk optimization, wavefront correction using the IFS image, lenslet tolerancing, the required spectral resolution to fit exoplanet atmospheres, and the utility of the spectrum in achieving higher contrast detection limits.

Groff, Tyler D.

Ten-Meter Scale Topography and Roughness of Mars Exploration Rovers Landing Sites and Martian Polar Regions

The Mars Orbiter Camera (MOC) has been operating on board of the Mars Global Surveyor (MGS) spacecraft since 1998. It consists of three cameras - Red and Blue Wide Angle cameras (FOV=140 deg.) and Narrow Angle camera (FOV=0.44 deg.). The Wide Angle camera allows surface resolution down to 230 m/pixel and the Narrow Angle camera - down to 1.5 m/pixel. This work is a continuation of the project, which we have reported previously. Since then we have refined and improved our stereo correlation algorithm and have processed many more stereo pairs. We will discuss results of our stereo pair analysis located in the Mars Exploration rovers (MER) landing sites and address feasibility of recovering topography from stereo pairs (especially in the polar regions), taken during MGS 'Relay-16' mode.

Ivanov, Anton B.

Draft Plan for Characterizing Commercial Data Products in Support of Earth Science Research

This presentation introduces a draft plan for characterizing commercial data products for Earth science research. The general approach to the commercial product verification and validation includes focused selection of a readily available commercial remote sensing products that support Earth science research. Ongoing product verification and characterization will question whether the product meets specifications and will examine its fundamental properties, potential and limitations. Validation will encourage product evaluation for specific science research and applications. Specific commercial products included in the characterization plan include high-spatial-resolution multispectral (HSMS) imagery and LIDAR data products. Future efforts in this process will include briefing NASA headquarters and modifying plans based on feedback, increased engagement with the science community and refinement of details, coordination with commercial vendors and The Joint Agency Commercial Imagery Evaluation (JACIE) for HSMS satellite acquisitions, acquiring waveform LIDAR data and performing verification and validation.

Ryan, Robert E.

Geologic Mapping of the Martian Impact Crater Tooting

Tooting crater is approximately 29 km in diameters, is located at 23.4 deg N, 207.5 deg E and is classified as a multi-layered ejecta crater. Tooting crater is a very young crater, with an estimated age of 700,000 to 2M years. The crater formed on virtually flat lava flows within Amazonis Planitia where there appears to have been no major topographic features prior to the impact, so that we can measure ejecta thickness and cavity volume. In the past 12 months, the authors have: published their first detailed analysis of the geometry of the crater cavity and the distribution of the ejecta layers; refined the geologic map of the interior of Tooting crater through mapping of the cavity at a scale of 1:1100K; and continued the analysis of an increasing number of high resolution images obtained by the CTX and HiRISE instruments. Currently the authors seek to resolve several science issues that have been identified during this mapping, including: what is the origin of the lobate flows on the NW and SW rims of the crater?; how did the ejecta curtain break apart during the formation of the crater, and how uniform was the emplacement process for the ejecta layers; and, can we infer physical characteristics about the ejecta? Future study plans include the completion of a draft geologic map of Tooting crater and submission of it to the U.S. Geological survey for a preliminary review, publishing a second research paper on the detailed geology of the crater cavity and the distribution of the flows on the crater rim, and completing the map text for the 1:100K geologic map description of units at Tooting crater.

Mouginis-Mark, Peter

Uncovering grain and subgrain microstructure at the scale of additive manufacturing melt tracks with a scalable cellular automaton solidification model

Metal additive manufacturing, characterized by rapid solidification, yields refined grains with a distinctive cellular subgrain microstructure that plays a pivotal role in determining material properties. Due to the significant computational expense demanded to simulate the required physics with submicron spatial resolution, their numerical simulations have been limited to proof-of-concept studies to either 2D or small subregions of a melt pool. In this study, an open-source, scalable, solidification code, muMatScale, based on the cellular automaton method, has been developed to predict the grain and the underlying subgrain microstructure over an entire melt pool. The model incorporates flexible parallelization schemes, utilizing MPI and OpenMP GPU Offloading, in addition to appropriate multi-physics specific to non-equilibrium rapid solidification in AM. The impact of nucleation parameters on grain microstructures was investigated with a focus on grain size variations and morphology transitions. With selected nucleation parameters, the simulation predicted the grain size, subgrain morphology, crystallographic orientation, and microsegregation aligned with experimental measurements. The model demonstrates that epitaxial grain growth is a dominant factor at the melt pool boundary, influencing grain size variation under different grain sizes in the build plate while maintaining consistent primary dendrite arm spacing under identical thermal conditions. Here, the highly efficient numerical model enables large-scale simulations with a spatial resolution of 100 nm or less, unveiling unprecedented insights into thermal and solutal diffusion driven grain growth, and the subgrains with microsegregation within grains in 3D across scales. muMatScale will enable the linking of submicron length-scale microstructure to part-level material behavior by investigating fundamental solidification problems at the intercellular scale in many-track and many-layer builds.

36 MATERIALS SCIENCE

Optimality of Gradient-MUSIC for Spectral Estimation

We introduce the Gradient-MUSIC algorithm for estimating the unknown frequencies and amplitudes of a nonharmonic signal from noisy time samples. While the classical MUSIC algorithm performs a computationally expensive search over a fine grid, Gradient-MUSIC is significantly more efficient and eliminates the need for discretization over a fine grid by using optimization techniques. It coarsely scans the 1D landscape to find initialization simultaneously for all frequencies followed by parallelizable local refinement via gradient descent. We also analyze its performance when the noise level is sufficiently small and the signal frequencies are separated by at least 8π/m, where π/m is the standard resolution of this problem. Even though the 1D landscape is nonconvex, we prove a global convergence result for Gradient-MUSIC: coarse scanning provably finds suitable initialization and gradient descent converges at a linear rate. In addition to convergence results, we also upper bound the error between the true signal frequencies and amplitudes with those found by Gradient-MUSIC. For example, if the noise has $\ell^\infty$ norm at most ϵ, then the frequencies and amplitudes are recovered up to error at most Cϵ/m and Cϵ respectively, which are minimax optimal in m and ϵ. Our theory can also handle stochastic noise with performance guarantees under nonstationary independent Gaussian noise. Our main approach is a comprehensive geometric analysis of the landscape, a perspective that has not been explored before.

97 MATHEMATICS AND COMPUTING

In orbit performance of the Einstein Observatory/HEAO-2 Imaging Proportional Counter

After two years of orbital operation, the IPC - a soft X-ray (0.2-4 keV) position sensitive proportional counter - continues to perform well after some initial gain changes, and is the most widely used focal plane instrument of the Einstein Observatory. X-ray images of several thousand cosmic X-ray sources have been obtained with a resolution of about 1.5 arcmin (FWHM) with a 1-deg field. Particle backgrounds are less than expected. The calibration of the instrument with respect to gain variations and spatial linearity is being refined by in-flight measurements.

Gorenstein, P.

A thermal/nonthermal approach to solar flares

An approach for modeling solar flare high-energy emissions is developed in which both thermal and nonthermal particles coexist and contribute to the radiation. The thermal/nonthermal distribution function is interpreted physically by postulating the existence of DC sheets in the flare region. The currents then provide both primary plasma heating through Joule dissipation, and runaway electron acceleration. The physics of runaway acceleration is discussed. Several methods are presented for obtaining approximations to the thermal/nonthermal distribution function, both within the current sheets and outside of them. Theoretical hard x ray spectra are calculated, allowing for thermal bremsstrahlung from the heated plasma electrons impinging on the chromosphere. A simple model for hard x ray images of two-ribbon flares is presented. Theoretical microwave gyrosynchrotron spectra are calculated and analyzed, uncovering important new effects caused by the interplay of thermal and nonthermal particles. The theoretical spectra are compared with observed high resolution spectra of solar flares, and excellent agreement is found, in both hard x rays and microwaves. The future detailed application of this approach to solar flares is discussed, as are possible refinements to this theory.

Benka, Stephen G.

The Archaeology of Little Wood Creek: New Chronometric Evidence

This study reports on the establishment of viable dates for several major cultural components at the Little Wood Creek site on the upper Hudson in Fort Edward, New York. The original excavation in the mid-1980s (Grossman et al. 1990) resulted in the identification of two major periods of occupation, a deeply buried Transitional period sequence of living floors, and closer to the surface, and separated by circa five feet of sterile alluvium, a series of Late Woodland period pits and features. Both are overlain by the discovery of the southern bastion of Revolutionary War-era Fort Edward. Ambiguities in the original bulk radiocarbon dating of the site left it in chronological limbo with widely divergent determinations for both prehistoric occupation periods. New AMS dates from 10 samples, four Transitional period and six Late Woodland period assays, both refined the absolute chronology of the site complex and clarified several major issues in the cultural and environmental history of the region. Together, these two sets of dates, combined with recent high resolution environmental sequences, provide sufficient resolution to correlate the newly defined periods of occupation with major events in the pollen and climate record of eastern New York State.

chronology

Processing of High Resolution, Multiparametric Radar Data for the Airborne Dual-Frequency Precipitation Radar APR-2

Following the successful Precipitation Radar (PR) of the Tropical Rainfall Measuring Mission, a new airborne, 14/35 GHz rain profiling radar, known as Airborne Precipitation Radar - 2 (APR-2), has been developed as a prototype for an advanced, dual-frequency spaceborne radar for a future spaceborne precipitation measurement mission. . This airborne instrument is capable of making simultaneous measurements of rainfall parameters, including co-pol and cross-pol rain reflectivities and vertical Doppler velocities, at 14 and 35 GHz. furthermore, it also features several advanced technologies for performance improvement, including real-time data processing, low-sidelobe dual-frequency pulse compression, and dual-frequency scanning antenna. Since August 2001, APR-2 has been deployed on the NASA P3 and DC8 aircrafts in four experiments including CAMEX-4 and the Wakasa Bay Experiment. Raw radar data are first processed to obtain reflectivity, LDR (linear depolarization ratio), and Doppler velocity measurements. The dataset is then processed iteratively to accurately estimate the true aircraft navigation parameters and to classify the surface return. These intermediate products are then used to refine reflectivity and LDR calibrations (by analyzing clear air ocean surface returns), and to correct Doppler measurements for the aircraft motion. Finally, the the melting layer of precipitation is detected and its boundaries and characteristics are identifIed at the APR-2 range resolution of 30m. The resulting 3D dataset will be used for validation of other airborne and spaceborne instruments, development of multiparametric rain/snow retrieval algorithms and melting layer characterization and statistics.

airborne radar

The absolute radiometric calibration of the advanced very high resolution radiometer

The early results of an absolute radiometric calibration of the NOAA-9 AVHRR sensor indicate significant degradations in the response of bands 1 and 2 compared to prelaunch values. The results are currently in the process of being verified and it may be that refinements of the methodology will be in order as additional data sets are analyzed. The LANDSAT TM calibration used in this approach is known to be very precise and the Herman radiative transfer code, supplemented by the 5-S code for gaseous transmission, is reliable as well. The extent to which other steps in the analysis procedure give rise to uncertainties in the results is currently under investigation. Particular attention is being given to the geometric matching of the AVHRR and TM imagery, as well as to the spectral redistribution procedure. By taking advantage of a reasonably precise calibration of TM imagery acquired on the same day as the AVHRR data at White Sands, a promising approach to the in-orbit calibration of AVHRR sensors is being developed. Current efforts involve primarily the examination of additional test cases and the investigation of possible simplifications in the procedure through judicious use of atmospheric models.

Slater, P. N.

Toward Immersed Boundary Simulation of High Reynolds Number Flows

In the immersed boundary (IB) method, the surface of an object is reconstructed with forcing terms in the underlying flow field equations. The surface may split a computational cell removing the constraint of the near wall gridlines to be aligned with the surface. This feature greatly simplifies the grid generation process which is cumbersome and expensive in particular for structured grids and complex geometries. The IB method is ideally suited for Cartesian flow solvers. The flow equations written in Cartesian coordinates appear in a very simple form and several numerical algorithms can be used for an efficient solution of the equations. In addition, the accuracy of numerical algorithms is dependent on the underlying grid and it usually deteriorates when the grid deviates from a Cartesian mesh. The challenge for the IB method lies in the representation of the wall boundaries and in providing an adequate near wall flow field resolution. The issue of enforcing no-slip boundary conditions at the immersed surface has been addressed by several authors by imposing a local reconstruction of the solution. Initial work by Verzicco et al. was based on a simple linear, one-dimensional operator and this approach proved to be accurate for boundaries largely aligned with the grid lines. Majumdar et al. used various multidimensional and high order polynomial interpolations schemes. These high order schemes, however, are keen to introduce wiggles and spurious extrema. Iaccarino & Verzicco and Kalitzin & Iaccarino proposed a tri-linear reconstruction for the velocity components and the turbulent scalars. A modified implementation that has proven to be more robust is reported in this paper. The issue of adequate near wall resolution in a Cartesian framework can initially be addressed by using a non-uniform mesh which is stretched near the surface. In this paper, we investigate an unstructured approach for local grid refinement that utilizes Cartesian mesh features. The computation of high Reynolds number wall bounded flows is particularly challenging as it requires the consideration of thin turbulent boundary layers, i.e. near wall regions with large gradients of the flow field variables. For such flows, the representation of the wall boundary has a large impact on the accuracy of the computation. It is also critical for the robustness and convergence of the flow solver.

Kalitzin, Georgi

Assessing Hurricane Katrina Vegetation Damage at Stennis Space Center using IKONOS Image Classification Techniques

Hurricane Katrina inflicted widespread damage to vegetation in southwestern coastal Mississippi upon landfall on August 29, 2005. Storm damage to surface vegetation types at the NASA John C. Stennis Space Center (SSC) was mapped and quantified using IKONOS data originally acquired on September 2, 2005, and later obtained via a Department of Defense ClearView contract. NASA SSC management required an assessment of the hurricane s impact to the 125,000-acre buffer zone used to mitigate rocket engine testing noise and vibration impacts and to manage forestry and fire risk. This study employed ERDAS IMAGINE software to apply traditional classification techniques to the IKONOS data. Spectral signatures were collected from multiple ISODATA classifications of subset areas across the entire region and then appended to a master file representative of major targeted cover type conditions. The master file was subsequently used with the IKONOS data and with a maximum likelihood algorithm to produce a supervised classification later refined using GIS-based editing. The final results enabled mapped, quantitative areal estimates of hurricane-induced damage according to general surface cover type. The IKONOS classification accuracy was assessed using higher resolution aerial imagery and field survey data. In-situ data and GIS analysis indicate that the results compare well to FEMA maps of flooding extent. The IKONOS classification also mapped open areas with woody storm debris. The detection of such storm damage categories is potentially useful for government officials responsible for hurricane disaster mitigation.

Spruce, Joseph P.

Small Spacecraft for Planetary Atmospheric, Surface, and Interior Structure Using Radio Links

Scientific studies using spacecraft radio links have been conducted on almost every Solar System exploration mission in the past five decades and have led to numerous discoveries. Radio Science experiments have elucidated the thermal history of the Moon from high resolution gravitational field measurements, unveiled the interiors of Titan, Enceladus, Mercury, Phobos, Vesta and Ceres providing key evidence for identifying subsurface oceans on icy moons; sounded Titan, Saturn, and Pluto’s atmospheres, and refined models for the atmospheres, surfaces, and interior structure of Mars and Venus. A Juno experiment is in progress measuring the gravitational field of Jupiter to reveal its interior structures, as did a similar recent Cassini experiment with Saturn. Experiments at Mercury, the Jovian system, and other targets, are in development or planning phases. Over the next 30 years, significant advances in radio and laser link-science technologies, including nearly one order of magnitude improvement achievable in range-rate and range accuracy, could enable many new scientific breakthroughs. Future exploration concepts in many cases focus on applications of small spacecraft and can include spacecraft constellations for studies of atmospheric dynamics, interior structures, and surface properties. A set of science-enabling radio link technologies specific to small spacecraft instrumentation on future solar system missions are under study and development. Examples include field tests of radio scattering to determine soil properties, smallsat constellations for dense geographic and temporal atmospheric probing, small science-quality software-defined transponders, miniature ultra-stable oscillators, and advanced radio-metric calibrations at the Deep Space Network. This paper describes many of these technologies and their scientific applications.

Elliott, Harvey

Acoustic analysis using numerical solutions of the Navier-Stokes equations

Numerical solutions of the time-dependent compressible Navier-Stokes equations are employed to analyze the unsteady features of jet flows which contribute to jet noise. The turbulent-mass flux spectra are examined in detail in the numerical analysis, and MacCormack's explicit finite difference algorithm is used to solve the governing equations. The computational grid is based on an axisymmetric jet with simple exponential stretching used to optimize the resolution of critical unsteady flow features. The time-varying numerical data are analyzed, and the time averages of the velocity and turbulence-intensity profiles are shown to agree with experimental data. Grid refinement and longer run times improved the prediction of the experimental mass-flux spectra. The numerical procedure can be extended to 3D jet-flow configurations to study the related 3D unsteady flow features.

Scott, James N.