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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 325 records · Page 18

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NASA NTRS1996

Characterization of a calcium/calmodulin-dependent protein kinase homolog from maize roots showing light-regulated gravitropism

Roots of many species respond to gravity (gravitropism) and grow downward only if illuminated. This light-regulated root gravitropism is phytochrome-dependent, mediated by calcium, and inhibited by KN-93, a specific inhibitor of calcium/calmodulin-dependent protein kinase II (CaMK II). A cDNA encoding MCK1, a maize homolog of mammalian CaMK, has been isolated from roots of maize (Zea mays L.). The MCK1 gene is expressed in root tips, the site of perception for both light and gravity. Using the [35S]CaM gel-overlay assay we showed that calmodulin-binding activity of the MCK1 is abolished by 50 microM KN-93, but binding is not affected by 5 microM KN-93, paralleling physiological findings that light-regulated root gravitropism is inhibited by 50 microM KN-93, but not by 5 microM KN-93. KN-93 inhibits light-regulated gravitropism by interrupting transduction of the light signal, not light perception, suggesting that MCK1 may play a role in transducing light. This is the first report suggesting a physiological function for a CaMK homolog in light signal transduction.

NASA Program Space Biology↗

NASA NTRS1979

Pioneer Venus infrared radiometer - Design, implementation and preliminary results

The paper discusses the Pioneer Venus infrared radiometer design and operation. Its main function is to measure the thermal emission from the atmosphere at seven pressure levels above the Venus clouds, allowing a determination of the vertical temperature structure. In addition to the temperature sounding channels, there are two channels operating in the visible and near infrared to study the structure of the upper clouds, and a far infrared channel sensitive to water vapor in and above the clouds. The instrument can operate in four modes including a calibration sequence; by utilizing the spinning action of the spacecraft and short integration times, a substantial portion of the planet can be mapped within a 90 min data taking period centered about periapsis time. Temperature profiles retrieved during the course of the mission will clarify the dynamical processes in the upper atmosphere.

Martonchik, J. V.↗

NASA NTRS1991

Systolic array IC for genetic computation

Measuring similarities between large sequences of genetic information is a formidable task requiring enormous amounts of computer time. Geneticists claim that nearly two months of CRAY-2 time are required to run a single comparison of the known database against the new bases that will be found this year, and more than a CRAY-2 year for next year's genetic discoveries, and so on. The DNA IC, designed at HP-ICBD in cooperation with the California Institute of Technology and the Jet Propulsion Laboratory, is being implemented in order to move the task of genetic comparison onto workstations and personal computers, while vastly improving performance. The chip is a systolic (pumped) array comprised of 16 processors, control logic, and global RAM, totaling 400,000 FETS. At 12 MHz, each chip performs 2.7 billion 16 bit operations per second. Using 35 of these chips in series on one PC board (performing nearly 100 billion operations per second), a sequence of 560 bases can be compared against the eventual total genome of 3 billion bases, in minutes--on a personal computer. While the designed purpose of the DNA chip is for genetic research, other disciplines requiring similarity measurements between strings of 7 bit encoded data could make use of this chip as well. Cryptography and speech recognition are two examples. A mix of full custom design and standard cells, in CMOS34, were used to achieve these goals. Innovative test methods were developed to enhance controllability and observability in the array. This paper describes these techniques as well as the chip's functionality. This chip was designed in the 1989-90 timeframe.

Anderson, D.↗

NASA NTRS1994

Inventing and improving ribozyme function: rational design versus iterative selection methods

Two major strategies for generating novel biological catalysts exist. One relies on our knowledge of biopolymer structure and function to aid in the 'rational design' of new enzymes. The other, often called 'irrational design', aims to generate new catalysts, in the absence of detailed physicochemical knowledge, by using selection methods to search a library of molecules for functional variants. Both strategies have been applied, with considerable success, to the remodeling of existing ribozymes and the development of ribozymes with novel catalytic function. The two strategies are by no means mutually exclusive, and are best applied in a complementary fashion to obtain ribozymes with the desired catalytic properties.

Review, Tutorial↗

NASA NTRS1975

Digital command system

The correct processing of the Digital Command System (DCS), which provides a limited real-time means of controlling specific flight program functions, was verified. The ability of the flight program to correctly read and process DCS commands was verified. Tests made by the flight program after reading the contents of the command decoder register to establish the validity of the received data were verified through the following: DCS mode command verification, DCS data command verification, DCS data validation, and DCS error message. The operation of the following DCS commands accepted and processed by the flight program was tested: time base update, navigation update, generalized switch selector, memory dump, terminate, execute generalized maneuver, return to nominal timeline, ECS water control valve logic inhibit, execute maneuver, execute alternate sequence, targeting load, ladder magnitude limit, S-IVB/IU de-orbit, compressed data dump, and remove inhibit on the extraction maneuver.

Source record↗

NASA NTRS1992

A study of the stellar population in the Chamaeleon dark clouds

The properties of the stellar population in the Chamaeleon dark clouds are discussed. Spectral energy distributions, based on the extant photometric and spectroscopic data base and IRAS fluxes measured from coadded data taken at the position of each star, and spectral types allow placement of the stars in an H-R diagram. The age and mass distributions and the luminosity function for the Chamaeleon stars are compared to those in the Taurus-Auriga dark clouds and are found to be similar. A small subsample (eight of 36) of the Chamaeleon stars show unusual spectral energy distributions which seem best interpreted as arising from circumstellar disks whose inner regions (R(in)) is less than 30-50 stellar radii) area devoid of material. The X-ray properties of this sample of premain-sequence objects are compared to those of other premain-sequence samples, as well as to the Hyades and the Pleiades main-sequence stars.

Gauvin, Lisa S.↗

NASA NTRS2005

Control Program for an Optical-Calibration Robot

A computer program provides semiautomatic control of a moveable robot used to perform optical calibration of video-camera-based optoelectronic sensor systems that will be used to guide automated rendezvous maneuvers of spacecraft. The function of the robot is to move a target and hold it at specified positions. With the help of limit switches, the software first centers or finds the target. Then the target is moved to a starting position. Thereafter, with the help of an intuitive graphical user interface, an operator types in coordinates of specified positions, and the software responds by commanding the robot to move the target to the positions. The software has capabilities for correcting errors and for recording data from the guidance-sensor system being calibrated. The software can also command that the target be moved in a predetermined sequence of motions between specified positions and can be run in an advanced control mode in which, among other things, the target can be moved beyond the limits set by the limit switches.

Johnston, Albert↗

NASA NTRS1987

Method and apparatus for telemetry adaptive bandwidth compression

Methods and apparatus are provided for automatic and/or manual adaptive bandwidth compression of telemetry. An adaptive sampler samples a video signal from a scanning sensor and generates a sequence of sampled fields. Each field and range rate information from the sensor are hence sequentially transmitted to and stored in a multiple and adaptive field storage means. The field storage means then, in response to an automatic or manual control signal, transfers the stored sampled field signals to a video monitor in a form for sequential or simultaneous display of a desired number of stored signal fields. The sampling ratio of the adaptive sample, the relative proportion of available communication bandwidth allocated respectively to transmitted data and video information, and the number of fields simultaneously displayed are manually or automatically selectively adjustable in functional relationship to each other and detected range rate. In one embodiment, when relatively little or no scene motion is detected, the control signal maximizes sampling ratio and causes simultaneous display of all stored fields, thus maximizing resolution and bandwidth available for data transmission. When increased scene motion is detected, the control signal is adjusted accordingly to cause display of fewer fields. If greater resolution is desired, the control signal is adjusted to increase the sampling ratio.

Graham, Olin L.↗

NASA NTRS2020

Circulating miRNA Signature Predicts Health Risks Associated with Cancer and Spaceflight

Biological risks associated with space radiation and microgravity are major concerns for long-term space travel. Through a Systems Biology approach, our previous NASA work has shown both TGF signaling pathways and miRNAs have a critical impact on defining health risks with and without space irradiation. We hypothesize that circulating microRNA (miRNA) signatures are driving microvascular disease and muscle degeneration associated with accelerating aging and will be enhanced by exposure to the space environment (radiation and microgravity). We are investigating this hypothesis with both in vivo and in vitro models to test novel antagonist therapies to these miRNA signatures as countermeasures to reduce space radiation-induced health risks. A comprehensive Systems Biology approach is utilized to examine the influence by high atomic number by high (H) atomic number (Z) and energy (E) (HZE) irradiation. To simulate low-dose exposure due to galactic cosmic rays (GCR), we used ions, energy, and doses determined by a NASA consensus formula of 7 different ions to represent GCR (referred to as GCR sim model). To similate high-dose radiation exposure due to solar particle events (SPE), we used a solar particle event (SPE) sim model which gave a total dose of 1Gy protons with energy ranges from 50MeV to 150MeV. C57BL/6 wild-type female mice were utilized for the irradiations with our established simulated microgravity model (hindlimb suspension model) and an in vitro 3D microvasculature tissue model under simulated microgravity (clinostat) conditions was also irradiated. To expand on the circulating miRNA signature determined from our preliminary data, we determined a group of conserved miRNAs which are commonly being regulated in the majority of the organs and tissues throughout the host using our established techniques. MiRNA-sequencing was done on serum (at time of sacrifice), liver, heart, and muscle (soleus muscle) tissue for all radiation groups. Additional validation of the key miRNAs was performed by droplet digital PCR (ddPCR). This revealed a key circulating miRNA signature (consisting of multiple miRNAs) impacting cardiovascular and muscular disease risk. Further in vitro experiments with CRISPR/Cas9 system to knockout the key miRNA signatures, novel self-delivering antagomirs, overexpression of the miRNAs test the functional impact of the miRNA signatures on both microvascular disease and muscle degeneration due to space irradiation. The current work has started to allow the possible development of a novel minimally invasive miRNA based radioprotector to be used as a countermeasure for space radiation. Collectively, understanding of how whole body space radiation impacts microvascular and tissue degeneration through circulating miRNAs will greatly enhance health risk prognostication and provide possible new mechanisms for protection against space radiation. This work is supported by the Translational Research Institute through NASA Cooperative Agreement NNX16AO69A (T-0404) awarded to AB.

Beheshti, Afshin↗

NASA NTRS1995

Assessing potential targets of calcium action in light-modulated gravitropism

Light, through the mediation of the pigment phytochrome, modulates the gravitropic response of the shoots and roots of many plants. The transduction of both light and gravity stimuli appears to involve Ca(2+)-regulated steps, one or more of which may represent points of intersection between the two transduction chains. To be confident that Ca2+ plays a critical role in stimulus-response coupling for gravitropism, it will be important to identify specific targets of Ca2+ action whose function can be clearly linked to the regulation of growth. Calcium typically exerts its influence on cell metabolism through binding to and activating key regulatory proteins. The three best characterized of these proteins in plants are the calmodulins, calcium-dependent protein kinases, and annexins. In this review we summarize what is known about the structure and function of these proteins and speculate on how their activation by Ca2+ could influence the differential growth response of gravitropism.

Review, Tutorial↗

NASA NTRS1990

Rapid traversal of an image database using binary synthetic discriminant filters

Pattern recognition invariant to image rotations of up to 75 deg, using a single filter, has been demonstrated for binary synthetic discriminant function (BSDF) optical filters, suggesting their use in directed graph-arranged data bases which can be rapidly traversed by means of a filter-plane programmable spatial light modulator (SLM). The filter data base is arranged as a tree structure in which the root node filters are invariant to over 60 deg rotation, while the leaves are 5-deg invariant. Results are presented from experiments with BSDFs designed to recognize in-plane-rotated views of a Space Shuttle Orbiter. Using a magnetooptic SLM that is driven at 350 Hz in the filter plane, orientation identifications requiring less than 30 msec have been achieved after sequencing through only 10 BSDFs.

Reid, Max B.↗

NASA NTRS2000

Microgravity Disturbance Characterization of the Quench Module Insert (QMI) Phase Change Device (PCD)

The Materials Science Research Facility (MSRF) is a multi-user, multi-purpose facility for materials science research. One experiment within the MSRF will be the Quench Module Insert (QMI), a high-temperature furnace with unique capabilities for processing different classes of materials. The primary functions of the QMI furnace are to melt, directionally solidify, and quench metallic samples, providing data to aid in understanding the effects of the microgravity environment on the characteristics of these processed metals. The QMI houses sealed individual sample ampoules containing material to be processed. Quenching of the samples in the QMI furnace is accomplished by releasing low-melting-point metallic shoes into contact with the outside of the sample ampoule, dissipating heat and cooling the sample inside. The impact from this method of quench will induce sample vibrations which could be large enough to adversely affect sample quality. Utilizing breadboard hardware, the sample quench sequence, releasing the shoes, was conducted. Data was collected from accelerometers located on the breadboard sample cartridge, indicating the maximum acceleration achieved by the sample. The primary objective of the test described in this presentation was to determine the acceleration imparted on the sample by the shoe contact. From this information, the science community can better assess whether this method of quench will allow them to obtain the data they need.

Gattis, Christy↗

NASA NTRS2000

Fray, a Drosophila serine/threonine kinase homologous to mammalian PASK, is required for axonal ensheathment

Fray is a serine/threonine kinase expressed by the peripheral glia of Drosophila, whose function is required for normal axonal ensheathment. Null fray mutants die early in larval development and have nerves with severe swelling and axonal defasciculation. The phenotype is associated with a failure of the ensheathing glia to correctly wrap peripheral axons. When the fray cDNA is expressed in the ensheathing glia of fray mutants, normal nerve morphology is restored. Fray belongs to a novel family of Ser/Thr kinases, the PF kinases, whose closest relatives are the PAK kinases. Rescue of the Drosophila mutant phenotype with PASK, the rat homolog of Fray, demonstrates a functional homology among these proteins and suggests that the Fray signaling pathway is widely conserved.

NASA Discipline Neuroscience↗

NASA NTRS1993

Resolved stars in nearby galaxies: Ground-based photometry of M81

Using the Canada-France-Hawaii Telescope (CFHT) we have obtained three closely spaced epochs of calibrated Blue Violet Red Infrared (BVRI) CCD imaging of two fields in M81, each known to contain a thirty-day Cepheid. Calibrated BVRI photometry of the brightest stars in these fields is presented. The slope of the luminosity function from the brightest 3-4 mag of the main-sequence blue plume is consistent with similar determinations of the apparent luminosity function in other resolved galaxies, thereby removing the one potential deviation from universality noted by Freedman in a photographic study of luminosity functions in nearby resolved galaxies. Under the assumption that the two Cepheids are representative, a reddening-law fit to the multiwavelength BVRI period-luminosity moduli give a true distance modulus of (m-M)sub 0 = 27.79 mag for M81, corresponding to a linear distance of 3.6 Mpc. An error analysis shows that the derived true distance modulus has a random error of +/- 0.28 mag (due to the photometric uncertainties in the BVRI data), with a systematic uncertainty of +/- 0.10 mag (accounting for the combined effects of unknown phasing of the data points, and the unknown positioning of these particular stars within the Cepheid instabiliy strip).

Madore, Barry F.↗

NASA NTRS2017

A Simulation Based Investigation of High Latency Space Systems Operations

This study was the first in a series of planned tests to use physics-based subsystem simulations to investigate the interactions between a spacecraft's crew and a ground-based mission control center for vehicle subsystem operations across long communication delays. The simulation models the life support system of a deep space habitat. It contains models of an environmental control and life support system, an electrical power system, an active thermal control systems, and crew metabolic functions. The simulation has three interfaces: 1) a real-time crew interface that can be use to monitor and control the subsystems; 2) a mission control center interface with data transport delays up to 15 minute each way; and 3) a real-time simulation test conductor interface used to insert subsystem malfunctions and observe the interactions between the crew, ground, and simulated vehicle. The study was conducted at the 21st NASA Extreme Environment Mission Operations (NEEMO) mission. The NEEMO crew and ground support team performed a number of relevant deep space mission scenarios that included both nominal activities and activities with system malfunctions. While this initial test sequence was focused on test infrastructure and procedures development, the data collected in the study already indicate that long communication delays have notable impacts on the operation of deep space systems. For future human missions beyond cis-lunar, NASA will need to design systems and support tools to meet these challenges. These will be used to train the crew to handle critical malfunctions on their own, to predict malfunctions and assist with vehicle operations. Subsequent more detailed and involved studies will be conducted to continue advancing NASA's understanding of space systems operations across long communications delays.

Li, Zu Qun↗

NASA NTRS2017

A Simulation Based Investigation of High Latency Space Systems Operations

This study was the first in a series of planned tests to use physics-based subsystem simulations to investigate the interactions between a spacecraft's crew and a ground-based mission control center for vehicle subsystem operations across long communication delays. The simulation models the life support system of a deep space habitat. It contains models of an environmental control and life support system, an electrical power system, an active thermal control system, and crew metabolic functions. The simulation has three interfaces: 1) a real-time crew interface that can be use to monitor and control the subsystems; 2) a mission control center interface with data transport delays up to 15 minute each way; and 3) a real-time simulation test conductor interface used to insert subsystem malfunctions and observe the interactions between the crew, ground, and simulated vehicle. The study was conducted at the 21st NASA Extreme Environment Mission Operations (NEEMO) mission. The NEEMO crew and ground support team performed a number of relevant deep space mission scenarios that included both nominal activities and activities with system malfunctions. While this initial test sequence was focused on test infrastructure and procedures development, the data collected in the study already indicate that long communication delays have notable impacts on the operation of deep space systems. For future human missions beyond cis-lunar, NASA will need to design systems and support tools to meet these challenges. These will be used to train the crew to handle critical malfunctions on their own, to predict malfunctions, and to assist with vehicle operations. Subsequent more detailed and involved studies will be conducted to continue advancing NASA's understanding of space systems operations across long communications delays.

Li, Zu Qun↗

NASA NTRS2022

Evolution and folding of repeat proteins

Repeat proteins are made with tandem copies of similar amino acid stretches that fold into elongated architectures. These proteins constitute excellent model systems to investigate how evolution relates to structure, folding, and function. Here, we propose a scheme to map evolutionary information at the sequence level to a coarse-grained model for repeat-protein folding and use it to investigate the folding of thousands of repeat proteins. We model the energetics by a combination of an inverse Potts-model scheme with an explicit mechanistic model of duplications and deletions of repeats to calculate the evolutionary parameters of the system at the single-residue level. These parameters are used to inform an Ising-like model that allows for the generation of folding curves, apparent domain emergence, and occupation of intermediate states that are highly compatible with experimental data in specific case studies. We analyzed the folding of thousands of natural Ankyrin repeat proteins and found that a multiplicity of folding mechanisms are possible. Fully cooperative all-or-none transitions are obtained for arrays with enough sequence-similar elements and strong interactions between them, while noncooperative element-by-element intermittent folding arose if the elements are dissimilar and the interactions between them are energetically weak. Additionally, we characterized nucleation-propagation and multidomain folding mechanisms. We show that the global stability and cooperativity of the repeating arrays can be predicted from simple sequence scores.

Ezequiel A. Galpern↗

NASA NTRS2024

Discrete Event Simulation-Based Timeline Validation Using R2U2

The Gateway Vehicle Systems Manager (VSM), the top-level software control system in a distributed, hierarchical Autonomous System Management Architecture is, like most modern spacecraft software control systems, heavily data-driven. For example, schedules (timelines) will be developed on the ground and, due to the high degree of autonomy, contain complex procedures involving conditional branching, variable timing, and resource contention resolution. In order to verify that an uploaded timeline will function correctly, it is necessary to explore the feasible set of possible executions. While it is possible to test a timeline using a mission simulation, the complexity of the system and duration of a timeline limits the number of trials and therefore the test coverage. To address this problem, the VSM team is using a discrete event system model that can rapidly generate from a timeline sets of event sequences using Monte Carlo techniques. To achieve rapid and trustworthy checking of the event sequences, we use an offline version of the runtime model checking tool R2U2. This presentation describes the approach the VSM team is using to implement the discrete event simulation and evaluate event sequences using R2U2. The presentation will discuss: 1. Description of the timelines by VSM in the context of VSM operations 2. Expansion of a timeline into a sequence of atomic events 3. Adjustment, in the Monte Carlo environment, of an event sequence to account for uncertainty, external events, and failures 4. Definition of R2U2 input and mission-time linear temporal logic files 5. Generation and use of R2U2 verdict sequences 6. Lessons learned and future work

Verification↗