Results for “TIME DISCRIMINATION” — Page 18

NASA NTRS1984

CDTI target selection criteria

A Cockpit Display of Traffic Information (CDTI) is a cockpit instrument which provides information to the aircrew on the relative location of aircraft traffic in the vicinity of their aircraft (township). In addition, the CDTI may provide information to assist in navigation and in aircraft control. It is usually anticipated that the CDTI will be integrated with a horizontal situation indicator used for navigational purposes and/or with a weather radar display. In this study, several sets of aircraft traffic data are analyzed to determine statistics on the number of targets that will be displayed on a CDTI using various target selection criteria. Traffic data were obtained from an Atlanta Terminal Area Simulation and from radar tapes recorded at the Atlanta and Miami terminal areas. Results are given in the form of plots showing the average percentage of time (or probability) that an aircraft equipped with a CDTI would observe from 0 to 10 other aircraft on the display for range settings on the CDTI up to 30 n. mi. and using various target discrimination techniques.

Britt, C. L.↗

NASA NTRS1985

Segmented Detector for Cosmic Gamma Rays

Segmented structure distinguishes gamma rays of cosmic origin from internal radioactivity. Preamplifier and discriminator channel connects each electrically independent segment of gamma-ray detector to logic circuitry. Logic compares and analyzes events in segments to determine sources. According to Monte Carlo calculations, sensitivity of detector two to three times better than conventional detectors because background interactions discerned and eliminated.

Varnell, L. S.↗

NASA NTRS1998

DC-8 Scanning Lidar Characterization of Aircraft Contrails and Cirrus Clouds

An angular-scanning large-aperture (36 cm) backscatter lidar was developed and deployed on the NASA DC-8 research aircraft as part of the SUCCESS (Subsonic Aircraft: Contrail and Cloud Effects Special Study) program. The lidar viewing direction could be scanned continuously during aircraft flight from vertically upward to forward to vertically downward, or the viewing could be at fixed angles. Real-time pictorial displays generated from the lidar signatures were broadcast on the DC-8 video network and used to locate clouds and contrails above, ahead of, and below the DC-8 to depict their spatial structure and to help select DC-8 altitudes for achieving optimum sampling by onboard in situ sensors. Several lidar receiver systems and real-time data displays were evaluated to help extend in situ data into vertical dimensions and to help establish possible lidar configurations and applications on future missions. Digital lidar signatures were recorded on 8 mm Exabyte tape and generated real-time displays were recorded on 8mm video tape. The digital records were transcribed in a common format to compact disks to facilitate data analysis and delivery to SUCCESS participants. Data selected from the real-time display video recordings were processed for publication-quality displays incorporating several standard lidar data corrections. Data examples are presented that illustrate: (1) correlation with particulate, gas, and radiometric measurements made by onboard sensors, (2) discrimination and identification between contrails observed by onboard sensors, (3) high-altitude (13 km) scattering layer that exhibits greatly enhanced vertical backscatter relative to off-vertical backscatter, and (4) mapping of vertical distributions of individual precipitating ice crystals and their capture by cloud layers. An angular scan plotting program was developed that accounts for DC-8 pitch and velocity.

Uthe, Edward E.↗

NASA NTRS1997

Plural-wavelength flame detector that discriminates between direct and reflected radiation

A flame detector employs a plurality of wavelength selective radiation detectors and a digital signal processor programmed to analyze each of the detector signals, and determine whether radiation is received directly from a small flame source that warrants generation of an alarm. The processor's algorithm employs a normalized cross-correlation analysis of the detector signals to discriminate between radiation received directly from a flame and radiation received from a reflection of a flame to insure that reflections will not trigger an alarm. In addition, the algorithm employs a Fast Fourier Transform (FFT) frequency spectrum analysis of one of the detector signals to discriminate between flames of different sizes. In a specific application, the detector incorporates two infrared (IR) detectors and one ultraviolet (UV) detector for discriminating between a directly sensed small hydrogen flame, and reflections from a large hydrogen flame. The signals generated by each of the detectors are sampled and digitized for analysis by the digital signal processor, preferably 250 times a second. A sliding time window of approximately 30 seconds of detector data is created using FIFO memories.

Hall, Gregory H.↗

NASA NTRS2013

Detection of Only Viable Bacterial Spores Using a Live/Dead Indicator in Mixed Populations

This method uses a photoaffinity label that recognizes DNA and can be used to distinguish populations of bacterial cells from bacterial spores without the use of heat shocking during conventional culture, and live from dead bacterial spores using molecular-based methods. Biological validation of commercial sterility using traditional and alternative technologies remains challenging. Recovery of viable spores is cumbersome, as the process requires substantial incubation time, and the extended time to results limits the ability to quickly evaluate the efficacy of existing technologies. Nucleic acid amplification approaches such as PCR (polymerase chain reaction) have shown promise for improving time to detection for a wide range of applications. Recent real-time PCR methods are particularly promising, as these methods can be made at least semi-quantitative by correspondence to a standard curve. Nonetheless, PCR-based methods are rarely used for process validation, largely because the DNA from dead bacterial cells is highly stable and hence, DNA-based amplification methods fail to discriminate between live and inactivated microorganisms. Currently, no published method has been shown to effectively distinguish between live and dead bacterial spores. This technology uses a DNA binding photoaffinity label that can be used to distinguish between live and dead bacterial spores with detection limits ranging from 109 to 102 spores/mL. An environmental sample suspected of containing a mixture of live and dead vegetative cells and bacterial endospores is treated with a photoaffinity label. This step will eliminate any vegetative cells (live or dead) and dead endospores present in the sample. To further determine the bacterial spore viability, DNA is extracted from the spores and total population is quantified by real-time PCR. The current NASA standard assay takes 72 hours for results. Part of this procedure requires a heat shock step at 80 degC for 15 minutes before the sample can be plated. Using a photoaffinity label would remove this step from the current assay as the label readily penetrates both live and dead bacterial cells. Secondly, the photoaffinity label can only penetrate dead bacterial spores, leaving behind the viable spore population. This would allow for rapid bacterial spore detection in a matter of hours compared to the several days that it takes for the NASA standard assay.

Behar, Alberto E.↗

NASA NTRS1994

Constraints on Neural Mechanisms Underlying the Spatial Integration of Speed Information

We measured human ability to integrate speed information presented simultaneously at multiple locations in the visual field. Observers did a two-interval forced-choice speed discrimination task with n grating patches in each interval at 4' eccentricity from fixation. In the first (integration) paradigm, all gratings in each interval moved at the same speed and observers were asked to pick the interval containing the faster gratings. Speed discrimination improved as the number of gratings in each interval increased. The observed decrease in threshold is not due simply to an increase in the effective area because performance with a single grating two, four, or six times the area of the original grating showed no such improvement. Furthermore, the improvement with n was still observed even when the speeds of the individual gratings in each interval were independent samples from a Gaussian distribution and the grating directions were balanced (i.e. equal numbers of gratings moved to the left and right). These results indicate that the neural mechanisms responsible for the integration of speed information act as if each patch provides an independent sample of speed, independent of grating direction. The results are not consistent with simple summation across space by a directionally selective mechanism with a large receptive field. In the second (search) paradigm, only one of the gratings in an interval moved faster and observers were asked to pick this interval. In this case, thresholds increased with the number of distractor gratings. The decrease in performance with increased n is common in search tasks and is often attributed to a bottleneck in the information that observers can process simultaneously. However, the results in the integration paradigm show that subjects indeed have access to and can use additional information with increasing n, at least up to 4 patches. Finally, simple detection models predict the trends in both the search and integration paradigms without invoking an input bottleneck.

Verghese, Preeti↗

NASA NTRS1994

Constraints on Neural Mechanisms Underlying the Spatial Integration of Speed Information

We measured human ability to integrate speed information presented simultaneously at multiple locations in the visual field. Observers did a two-interval forced-choice speed discrimination task with n grating patches in each interval at 4deg eccentricity from fixation. In the first (integration) paradigm, all gratings in each interval moved at the same speed and observers were asked to pick the interval containing the faster gratings. Speed discrimination improved as the number of gratings in each interval increased. The observed decrease in threshold is not due simply to an increase in the effective area because performance with a single grating two, four, or six times the area of the original grating showed no such improvement. Furthermore, the improvement with n was still observed even when the speeds of the individual gratings in each interval were independent samples from a Gaussian distribution and the grating directions were balanced (i.e. equal numbers of gratings moved to the left and right). These results indicate that the neural mechanisms responsible for the integration of speed information act as if each patch provides an independent sample of speed, independent of grating direction. The results are not consistent with simple summation across space by a directionally selective mechanism with a large receptive field. in the second (search) paradigm, only one of the gratings in an interval moved faster and observers were asked to pick this interval. In this case, thresholds increased with the number of distractor gratings. The decrease in performance with increased n is common in search tasks and is often attributed to a bottleneck in the information that observers can process simultaneously. However, the results in the integration paradigm show that subjects indeed have access to and can use additional information with increasing n, at least up to 4 patches. Finally, simple detection models predict the trends in both the search and integration paradigms without invoking an input bottleneck.

Verghese, Preeti↗

NASA NTRS2010

Direct Relationship Between Perceptual and Motor Variability

The time that elapses between stimulus onset and the onset of a saccadic eye movement is longer and more variable than can be explained by neural transmission times and synaptic delays (Carpenter, 1981, in: Eye Movements: Cognition & Visual Perception, Earlbaum). In theory, noise underlying response-time (RT) variability could arise at any point along the sensorimotor cascade, from sensory noise arising Vvithin the early visual processing shared Vvith perception to noise in the motor criterion or commands necessary to trigger movements. These two loci for internal noise can be distinguished empirically; sensory internal noise predicts that response time Vvill correlate Vvith perceived stimulus magnitude whereas motor internal noise predicts no such correlation. Methods. We used the data described by Liston and Stone (2008, JNS 28:13866-13875), in which subjects performed a 2AFC saccadic brightness discrimination task and the perceived brightness of the chosen stimulus was then quantified in a second 21FC perceptual task. Results. We binned each subject's data into quartiles for both signal strength (from dimmest to brightest) and RT (from slowest to fastest) and analyzed the trends in perceived brightness. We found significant effects of both signal strength (as expected) and RT on normalized perceived brightness (both p less than 0.0001, 2-way ANOVA), without significant interaction (p = 0.95, 2-way ANOVA). A plot of normalized perceived brightness versus normalized RT show's that more than half of the variance was shared (r2 = 0.56, P less than 0.0001). To rule out any possibility that some signal-strength related artifact was generating this effect, we ran a control analysis on pairs of trials with repeated presentations of identical stimuli and found that stimuli are perceived to be brighter on trials with faster saccades (p less than 0.001, paired t-test across subjects). Conclusion. These data show that shared early visual internal noise jitters perceived brightness and the saccadic motor output in parallel. While the present correlation could theoretically result, either directly or indirectly, from some low-level brainstem or retinal mechanism (e.g., arousal, pupil size, photoreceptor noise) that influences both visual and oculomotor circuits, this is unlikely given the earlier fin ding that the variability in perceived motion direction and smooth-pursuit motor output is highly correlated (Stone and Krauzlis, 2003, JOV 3:725-736), suggesting that cortical circuits contribute to the shared internal noise.

Liston, Dorion B.↗

NASA NTRS1971

Earth orbiting Sisyphus system study

The feasibility of employing an optical meteoroid detecting system, known as Sisyphus, to measure the near-earth particulates from an earth orbiting vehicle, is considered. A Sisyphus system can discriminate between natural and man-made particles since the system measures orbital characteristics of particles. A Sisyphus system constructed for the Pioneer F/G missions to Jupiter is used as the baseline, and is described. The amount of observing time which can be obtained by a Sisyphus instrument launched into various orbits is determined. Observation time is lost when, (1) the Sun is in or near the field of view, (2) the lighted Earth is in or near the field of view, (3) the instrument is eclipsed by the Earth, and (4) the phase angle measured at the particle between the forward scattering direction and the instrument is less than a certain critical value. The selection of the launch system and the instrument platform with a dedicated, attitude controlled payload package is discussed. Examples of such systems are SATS and SOLRAD 10(C) vehicles, and other possibilities are AVCO Corp. S4 system, the OWL system, and the Delta Payload Experiment Package.

Jurkevich, I.↗

NASA NTRS2014

Integration of RGB "Dust" Imagery to Operations at the Albuquerque Forecast Office

The NASA/Short‐term Prediction, Research, and Transition (SPoRT) Program has been providing unique Red‐Green‐Blue (RGB) composite imagery to its operational partners since 2005. In the early years of activity these RGB products were related to a True Color RGB, showing what one's own eyes would see if looking down at earth from space, as well as a Snow‐Cloud RGB (i.e. False Color), separating clouds from snow on the ground. More recently SPoRT has used the EUMETSAT Best Practices standards for RGB composites to transition a wide array of imagery for multiple uses. A "Dust" RGB product has had particular use at the Albuquerque, New Mexico WFO. Several cases have occurred where users were able to isolate dust plume locations for mesoscale and microscale events during day and night time conditions. In addition the "Dust" RGB can be used for more than just detection of dust as it is sensitive to the changes in density due to atmospheric moisture content. Hence low‐level dry boundaries can often be discriminated. This type of imagery is a large change from the single channel imagery typically used by operational forecast staff and hence, can be a challenge to interpret. This presentation aims to discuss the integration of such new imagery into operational use as well as the benefits assessed by the Albuquerque WFO over several documented events.

Fuell, Kevin↗

NASA NTRS2014

Integration of RGB "Dust" Imagery to Operations at the Albuqueque Forecast Office

The NASA/Short‐term Prediction, Research, and Transition (SPoRT) Program has been providing unique Red‐Green‐Blue (RGB) composite imagery to its operational partners since 2005. In the early years of activity these RGB products were related to a True Color RGB, showing what one's own eyes would see if looking down at earth from space, as well as a Snow‐Cloud RGB (i.e. False Color), separating clouds from snow on the ground. More recently SPoRT has used the EUMETSAT Best Practices standards for RGB composites to transition a wide array of imagery for multiple uses. A "Dust" RGB product has had particular use at the Albuquerque, New Mexico WFO. Several cases have occurred where users were able to isolate dust plume locations for mesoscale and microscale events during day and night time conditions. In addition the "Dust" RGB can be used for more than just detection of dust as it is sensitive to the changes in density due to atmospheric moisture content. Hence low‐level dry boundaries can often be discriminated. This type of imagery is a large change from the single channel imagery typically used by operational forecast staff and hence, can be a challenge to interpret. This presentation aims to discuss the integration of such new imagery into operational use as well as the benefits assessed by the Albuquerque WFO over several documented events.

Fuell, Kevin↗

NASA NTRS2025

Magnetic Resonance Imaging (MRI) to Assess Changes to Trabecular Microarchitecture (Trb µArch) of the Hip

BACKGROUND . Calculated loss rates in trabecular (Trb) volumetric bone mineral density (vBMD), in astronauts after return from 6-month long-duration (LD) spaceflight, are rapid relative to menopause-induced loss rates and could contribute to the observed lack of recovery. Perforations in the Trb bony struts, skeletal fragility and fractures [5] have been associated with the rapid rate of bone loss in women due to menopause. The more rapid loss rate observed in astronauts could be inducing similar, irreversible losses of trabecular connectivity as seen in cadaver studies of female skeletons. These changes are not detectable by DXA technology and would not be discovered in astronauts during scheduled, triennial DXA testing for primary osteoporosis. High-resolution-peripheral quantitative computed tomography (HR-pQCT) of the lower leg have detected losses in Trb vBMD (vBMD) and changes in Trb microarchitecture (µArch) in astronauts immediately following routine 6-month spaceflights and continuing in some individuals for 1 or more years postflight with no signs of recovery. Resolution of these microarchitectural changes by whole body QCT, especially in the deeply embedded hip bone, is prohibitive due to excessive and unsafe radiation exposures. While HR-pQCT has sufficient resolution to study Trb µArch at safe and lower radiation exposure testing is restricted only to the lower leg and wrist. Hence, a technology to detect detrimental changes to Trb µArch in astronauts, especially of the hip, is needed to inform type and timing of countermeasures to irreversible deficits (preflight, inflight and postflight). METHODS . This HRP-funded study (2023-2027) will verify the sensitivity of a protocol for MRI scanning (on a 3T Siemens Vida) to detect changes to hip Trb µArch in persons following spinal cord injury (SCI); skeletal immobilization below the spinal lesion will serve as an analog for non-weight-bearing during spaceflight. The study period has been extended by 1 yr due to delayed Richmond IRB approval in Y1. A 3T MRI protocol will characterize serial changes in hip Trb µArch in SCI patients from time of admission at the Richmond VA Medical Center to their scheduled annual exam 12-months later. SCI patients with age, sex, and physical characteristics like ISS astronauts will be consented. The ability of 3T MRI hip scan to discriminate loss of trabecular connectivity subjects with SCI will be compared to age- and sex-matched ambulatory controls. A comparison between HR-pQCT and MRI measurements at the ankle (distal tibia) of study subjects will also be performed. A modified DXA scan analysis (3D-Shaper) will also be verified as a potential method for monitoring concurrent changes in hip Trb bone over 12-month. SIGNIFICANCE . Deliverables: a surveillance method for assessing for irreversible losses of connectivity and how skeletal changes after LD spaceflight(s) would modify the terrestrial risk of primary osteoporosis.

Spinal Cord Injury↗

NASA NTRS2025

Magnetic Resonance Imaging (MRI) to Assess Changes to Trabecular Microarchitecture (Trb µArch) of the Hip

BACKGROUND . Calculated loss rates in trabecular (Trb) volumetric bone mineral density (vBMD), in astronauts after return from 6-month long-duration (LD) spaceflight, are rapid relative to menopause-induced loss rates and could contribute to the observed lack of recovery. Perforations in the Trb bony struts, skeletal fragility and fractures [5] have been associated with the rapid rate of bone loss in women due to menopause. The more rapid loss rate observed in astronauts could be inducing similar, irreversible losses of trabecular connectivity as seen in cadaver studies of female skeletons. These changes are not detectable by DXA technology and would not be discovered in astronauts during scheduled, triennial DXA testing for primary osteoporosis. High-resolution-peripheral quantitative computed tomography (HR-pQCT) of the lower leg have detected losses in Trb vBMD (vBMD) and changes in Trb microarchitecture (µArch) in astronauts immediately following routine 6-month spaceflights and continuing in some individuals for 1 or more years postflight with no signs of recovery. Resolution of these microarchitectural changes by whole body QCT, especially in the deeply embedded hip bone, is prohibitive due to excessive and unsafe radiation exposures. While HR-pQCT has sufficient resolution to study Trb µArch at safe and lower radiation exposure testing is restricted only to the lower leg and wrist. Hence, a technology to detect detrimental changes to Trb µArch in astronauts, especially of the hip, is needed to inform type and timing of countermeasures to irreversible deficits (preflight, inflight and postflight). METHODS . This HRP-funded study (2023-2027) will verify the sensitivity of a protocol for MRI scanning (on a 3T Siemens Vida) to detect changes to hip Trb µArch in persons following spinal cord injury (SCI); skeletal immobilization below the spinal lesion will serve as an analog for non-weight-bearing during spaceflight. The study period has been extended by 1 yr due to delayed Richmond IRB approval in Y1. A 3T MRI protocol will characterize serial changes in hip Trb µArch in SCI patients from time of admission at the Richmond VA Medical Center to their scheduled annual exam 12-months later. SCI patients with age, sex, and physical characteristics like ISS astronauts will be consented. The ability of 3T MRI hip scan to discriminate loss of trabecular connectivity subjects with SCI will be compared to age- and sex-matched ambulatory controls. A comparison between HR-pQCT and MRI measurements at the ankle (distal tibia) of study subjects will also be performed. A modified DXA scan analysis (3D-Shaper) will also be verified as a potential method for monitoring concurrent changes in hip Trb bone over 12-month. SIGNIFICANCE . Deliverables: a surveillance method for assessing for irreversible losses of connectivity and how skeletal changes after LD spaceflight(s) would modify the terrestrial risk of primary osteoporosis.

Risk Surveillance↗

NASA NTRS1996

An optimized index of human cardiovascular adaptation to simulated weightlessness

Prolonged exposure to weightlessness is known to produce a variety of cardiovascular changes, some of which may influence the astronaut's performance during a mission. In order to find a reliable indicator of cardiovascular adaptation to weightlessness, we analyzed data from nine male subjects after a 24-hour period of normal activity and after a period of simulated weightlessness produced by two hours in a launch position followed by 20 hours of 6 degrees head-down tilt plus pharmacologically induced diuresis (furosemide). Heart rate, arterial pressure, thoracic fluid index, and radial flow were analyzed. Autoregressive spectral estimation and decomposition were used to obtain the spectral components of each variable from the subjects in the supine position during pre- and post-simulated weightlessness. We found a significant decrease in heart rate power and an increase in thoracic fluid index power in the high frequency region (0.2-0.45 Hz) and significant increases in radial flow and arterial pressure powers in the low frequency region (<0.2 Hz) in response to simulated weightlessness. However, due to the variability among subjects, any single variable appeared limited as a dependable index of cardiovascular adaptation to weightlessness. The backward elimination algorithm was then used to select the best discriminatory features from these spectral components. Fisher's linear discriminant and Bayes' quadratic discriminant were used to combine the selected features to obtain an optimal index of adaptation to simulated weightlessness. Results showed that both techniques provided improved discriminant performance over any single variable and thus have the potential for use as an index to track adaptation and prescribe countermeasures to the effects of weightlessness.

NASA Discipline Number 14-10↗

NASA NTRS1988

Laser agile illumination for object tracking and classification - Feasibility study

The 'agile illumination' concept for discrimination between ICBM warheads and decoys involves a two-aperture illumination with coherent light, diffraction of light by propagation, and a resulting interference pattern on the object surface. A scanning two-beam interference pattern illuminates one object at a time; depending on the shape, momentum, spinning, and tumbling characteristics of the interrogated object, different temporal signals will be obtained for different classes of objects.

Scholl, Marija S.↗

NASA NTRS1997

Adaptive Optics Imaging of Solar System Objects

Most solar system objects have never been observed at wavelengths longer than the R band with an angular resolution better than 1 sec. The Hubble Space Telescope itself has only recently been equipped to observe in the infrared. However, because of its small diameter, the angular resolution is lower than that one can now achieved from the ground with adaptive optics, and time allocated to planetary science is limited. We have been using adaptive optics (AO) on a 4-m class telescope to obtain 0.1 sec resolution images solar system objects at far red and near infrared wavelengths (0.7-2.5 micron) which best discriminate their spectral signatures. Our efforts has been put into areas of research for which high angular resolution is essential, such as the mapping of Titan and of large asteroids, the dynamics and composition of Neptune stratospheric clouds, the infrared photometry of Pluto, Charon, and close satellites previously undetected from the ground.

Roddier, Francois↗

NASA NTRS1992

Linear Spectral Analysis of Plume Emissions Using an Optical Matrix Processor

Plume spectrometry provides a means to monitor the health of a burning rocket engine, and optical matrix processors provide a means to analyze the plume spectra in real time. By observing the spectrum of the exhaust plume of a rocket engine, researchers have detected anomalous behavior of the engine and have even determined the failure of some equipment before it would normally have been noticed. The spectrum of the plume is analyzed by isolating information in the spectrum about the various materials present to estimate what materials are being burned in the engine. Scientists at the Marshall Space Flight Center (MSFC) have implemented a high resolution spectrometer to discriminate the spectral peaks of the many species present in the plume. Researchers at the Stennis Space Center Demonstration Testbed Facility (DTF) have implemented a high resolution spectrometer observing a 1200-lb. thrust engine. At this facility, known concentrations of contaminants can be introduced into the burn, allowing for the confirmation of diagnostic algorithms. While the high resolution of the measured spectra has allowed greatly increased insight into the functioning of the engine, the large data flows generated limit the ability to perform real-time processing. The use of an optical matrix processor and the linear analysis technique described below may allow for the detailed real-time analysis of the engine's health. A small optical matrix processor can perform the required mathematical analysis both quicker and with less energy than a large electronic computer dedicated to the same spectral analysis routine.

Gary, C. K.↗

NASA NTRS2012

Phytoplankton Assemblage Patterns in the Southern Mid-Atlantic Bight

As part of the Wallops Coastal Oceans Observing Laboratory (Wa-COOL) Project, we sampled a time-series transect in the southern Mid-Atlantic Bight (MAB) biweekly. Our 2-year time-series data included physical parameters, nutrient concentrations, and chlorophyll a concentrations. A detailed phytoplankton assemblage structure was examined in the second year. During the 2-year study, chlorophyll a concentration (and ocean color satellite imagery) indicated that phytoplankton blooms occurred in January/February during mixing conditions and in early autumn under stratified conditions. The chlorophyll a concentrations ranged from 0.25 microgram 1(exp -1) to 15.49 microgram 1(exp -1) during the 2-year period. We were able to discriminate approximately 116 different species under phase contrast microscopy. Dominant phytoplankton included Skeletonema costatum, Rhizosolenia spp., and Pseudo-nitzschia pungens. In an attempt to determine phytoplankton species competition/succession within the assemblage, we calculated a Shannon Weaver diversity index for our diatom microscopy data. Diatom diversity was greatest during the winter and minimal during the spring. Diatom diversity was also greater at nearshore stations than at offshore stations. Individual genera appeared patchy, with surface and subsurface patches appearing abruptly and persisting for only 1-2 months at a time. The distribution of individual species differed significantly from bulk variables of the assemblage (chlorophyll a ) and total phytoplankton assemblage (cells), which indicates that phytoplankton species may be limited in growth in ways that differ from those of the total assemblage. Our study demonstrated a highly diverse phytoplankton assemblage throughout the year, with opportunistic species dominating during spring and fall in response to seasonal changes in temperature and nutrients in the southern MAB.

Mid-Atlantic↗