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Toward a standardization of cryostructure and cryogenic soil structure terminology for the field description of permafrost‐affected soils

This paper establishes standardized terminology and field documentation protocols for cryostructures and cryogenic soil structures in permafrost‐affected soils and provides brief guidance on descriptions of ground ice morphology and ice volume estimates. We consolidate permafrost terminology from Russian and North American literature, clarify long‐standing ambiguities, and provide explicit guidelines that align with US Department of Agriculture‐Natural Resources Conservation Service soil description standards. Our scheme makes critical distinctions between cryostructure, the distribution of ice within soil, and cryogenic soil structure, the morphological structure of soil resulting from ice formation. The scheme organizes cryostructures into three main categories: non‐segregated ice, visible segregated ice, and ice matrices. We introduce standardized codes and parameters for field descriptions of ice and soil that enable machine‐readable data collection compatible with existing soil information systems. This standardization will significantly enhance the integration of field observations into landscape‐scale assessments of permafrost stability, infrastructure vulnerability, and ecosystem response to permafrost thaw, addressing an urgent need for quantitative data to inform modeling and decision‐making in rapidly changing Arctic and subarctic environments.

Andersen, Megan L. [University of Minnesota, Saint↗

Guide to Developing Energy Services Interfaces

This document provides guidance to develop communications interfaces that are highly interoperable and adhere to a set of energy services interface (ESI) principles. It can be applied in the context of information and communications technology interface standards and implementation profiles that enable the integration of a facility with responsive distributed energy resources (DER) into an electric power system consistent with the fundamental ESI principles. In this context, a DER facility may consist of a single DER with a communicating controller or may be as complex as a microgrid campus with several buildings and many DERs. This document is not a technical interface standard, but the requirements described can be used to check that existing, augmented, or new interface standards meet the interoperability requirements of the ESI concept. In this way, this guide for the ESI specification can support future advancements in developing interoperability standards and implementation profiles. The intended audience for this document is DER integration practitioners and ecosystem groups that are in the business of developing and implementing communications interfaces for DER integration. It is intended that these groups apply the information and processes in this document to develop communications standards and implementation profiles that are consistent with fundamental ESI principles.

2030.5↗

Austin Sustainable and Holistic Integration of Energy Storage and Solar PV [Austin SHINES]. Final Report, Version 2

The Austin SHINES project and solution is a software management platform, for an electric grid with a high penetration of dispersed photovoltaic (PV) solar generation sites, which maintains the traditional power quality and reliability associated with grid service. This project developed and deployed the platform as a Distributed Energy Resource Management System (DERMS), engaging multiple advanced controls, to evaluate operation and optimization of a fleet of diverse DER assets, installed at several locations among Austin Energy’s customers and distribution system. The project also produced a methodology to create a replicable DERMS template, adaptable to other regions and market structures. Last, Austin SHINES aimed to demonstrate the solution’s methodology would enable the DER grid ecosystem to serve load at a technical cost (System Levelized Cost of Electricity, or System LCOE) of less than the U.S. Department of Energy SHINES program metric of $0.14/kWh, in a defined boundary, while enabling a high penetration of distributed PV. Research was categorized in 6 reports (Final Deliverables = FD) listed below, with titles and descriptions indicating which area of understanding was investigated: FD-1: System Levelized Cost of Electricity (System LCOE) Methodology The creation and use of the System LCOE to Serve Load metric that encompasses the holistic, system-level costs and benefits of all resources, and enables them to be evaluated based on their ability to support an efficient and low-cost integrated grid ecosystem. FD-2: Software Platform Product Description The creation of new DER control methodologies deployable within a utility-grade software platform that enable DER's to maximize their benefit within a grid, that is capable of serving load enabling a high penetration of distributed PV generation. FD-3: Optimal Design Methodology Optimal design methodologies for individual DER installations that enable utilities to determine the optimal combinations and sizing for individual DER sites. FD-4: Austin SHINES Ownership and Operation Models for DER System Performance A comparison of multiple DER aggregation and ownership methodologies including direct utility control, third-party aggregator, and autonomous. FD-5: Economic Modeling & Optimization A comparison of multiple DER technology mixes and configurations within the distribution system, providing insight into an optimal blend of technologies that best enable the distribution system to serve load at the lowest cost at high penetrations of solar. FD-6: Fielded Assets Deployed DER assets within the Austin Energy SHINES circuits. Austin SHINES provided an opening for state-of-the-art technology products to be deployed, providing a rich opportunity for improving how each of the products perform as stand-alone products, and in concert with other complementary products. The Austin SHINES project comprised of two key metrics for System LCOE: SystemLCOE_SHINES<$0.14/kWh Modeled ΔSystemLCOE_SHINES/ΔSystemLCOE_Base≥20% at same solar penetration The System LCOE calculation uses the costs of the utility-owned infrastructure as it exists today, the cost of the DERs that exist in the system today, and the cost of the purchase of energy from ERCOT wholesale markets over the course of the calendar year. All costs are on an annualized basis. The capital and operating costs are derived from the rate case, which produces a yearly cost. The net cost of energy and services imported to the system is integrated over the test year, as is the load served and solar penetration. The first metric was easily achieved by every scenario considered. The goal was set when the Department of Energy’s SHINES Funding Opportunity Announcement was written in 2015 and was a more difficult target at the time. Due mostly to rapidly declining costs for DERs and the significant decrease in the Electric Reliability Council of Texas (ERCOT) energy market prices, which results in lower net cost of energy purchases, the System LCOE is well below this target for all scenarios considered. A fleet of DERs can assume different mixtures, each of which serves the load at a different LCOE. The optimal mixture of DERs serves load at the smallest System LCOE. The second metric (hereinafter %delta metric) asks that the holistic DERMS controls reduce the incremental cost above the baseline of going to a high solar penetration future by at least 20% as compared to the case of a DER deployment with no sophisticated controls (autonomous). Many comparison sets were created throughout this project. Physical technology was installed for informing utility engineering and testing several types of operational control schemes, through the DERMS. The types of operational control which were compared for valuation of the System LCOE Metric were: Holistic control = using the full suite of the DERMS platform to decide and optimize how/why the systems operate depending on weather, market, and reliability signal input. Autonomous control = a local mode at the asset site, wherein a schedule operates the asset, with visibility into performance only No control = the baseline for comparing value against the other two types of control The types of ownership control included: Direct Utility control = the utility dispatches a signal to each asset Third-Party Aggregator = a third party aggregates a fleet of assets and the utility dispatches one signal for all Autonomous = a local mode is set for operation at the asset site, wherein a schedule operates the asset, with visibility into performance only The types of control methodologies deployable within a utility-grade software platform included: Utility Peak Load Reduction = Lower transmission cost obligation Day-Ahead Energy Arbitrage = Realize economic value through price differential Real-Time Price Dispatch = Realize economic value from real-time price spikes Voltage support = Reduce losses and increase solar generation Distribution Congestion Management = Increase local grid reliability Demand Charge Reduction = Lower customer bills and realize system benefit The fielded assets deployed for the project were: Utility Scale Kingsbery Energy Storage System: 1.5 MW / 3 MWh Li-Ion battery storage Mueller Energy Storage System: 1.75 MW / 3.2 MWh Li-Ion battery storage, 7 Energy Storage Units (250 kW each) La Loma Community Solar: 2.6 MW Commercial Scale Aggregated storage installations at 3 sites, with existing solar (300+ kW): One 18 kW / 36 kWh Li-Ion battery storage Two 72 kW / 144 kWh Li-Ion battery storage Residential Scale Aggregated storage installations: -Six stationary battery storage systems (10 kWh each) at homes with existing solar -One Electric Vehicle installed as Vehicle-to-Grid (V2G) Utility-Controlled Solar via Smart Inverters at 12 homes Autonomously-Controlled Smart Inverters at 6 homes Over the course of the project, Austin SHINES undertook installing more than 3 MW of distributed battery energy storage, smart PV inverters, a DER control platform, and other enabling technologies utilizing customer and utility locations and aggregation models. All of these resources were to be integrated and optimized at the utility level. DER assets and control methodologies were designed to achieve a credible pathway to a System LCOE for energy delivered to load of $0.14//kWh or less by 2020, while maximizing distributed solar generation and maintaining acceptable standards of power quality. The project also established a template for other regions to follow, to maximize the adoption of distributed solar PV in support of an economic and efficient grid. In total, the Austin SHINES project added value to the DER subject area in each layer of integration. From utility, to commercial to residential scales, the sheer hierarchy of communication and coordination was a significant accomplishment in addition to learnings from what these communications revealed was unique to each. Economically, the most effective method demonstrated was the criticality of planning phases. Contingencies and multiple projection scenarios helped guide the project to deploy optimal design as close as feasible, in real world conditions. The project and reports will serve public benefit by outlining specific areas of DER strategy and installation where many stakeholders and needs can be addressed with improved efficiency. Overall, communities and utilities should use the results to guide the increasing options available for powering the grid with DER, renewables, and carbon considerate energy.

14 SOLAR ENERGY↗

Carbon cycling across ecosystem succession in a north temperate forest: Controls and management implications

Despite decades of progress, much remains unknown about successional trajectories of carbon (C) cycling in north temperate forests. Drivers and mechanisms of these changes, including the role of different types of disturbances, are particularly elusive. To address this gap, we synthesized decades of data from experimental chronosequences and long-term monitoring at a well-studied, regionally representative field site in northern Michigan, USA. Our study provides a comprehensive assessment of changes in above- and belowground ecosystem components over two centuries of succession, links temporal dynamics in C pools and fluxes with underlying drivers, and offers several conceptual insights to the field of forest ecology. Our first advance shows how temporal dynamics in some ecosystem components are consistent across severe disturbances that reset succession and partial disturbances that slightly modify it: both of these disturbance types increase soil N availability, alter fungal community composition, and alter growth and competitive interactions between short-lived pioneer and longer-lived tree taxa. Further, these changes in turn affect soil C stocks, respiratory emissions, and other belowground processes. Second, we show that some other ecosystem components have effects on C cycling that are not consistent over the course of succession. For example, canopy structure does not influence C uptake early in succession but becomes important as stands develop, and the importance of individual structural properties changes over the course of two centuries of stand development. Third, we show that in recent decades, climate change is masking or overriding the influence of community composition on C uptake, while respiratory emissions are sensitive to both climatic and compositional change. In synthesis, we emphasize that time is not a driver of C cycling; it is a dimension within which ecosystem drivers such as canopy structure, tree and microbial community composition change. Changes in those drivers, not in forest age, are what control forest C trajectories, and those changes can happen quickly or slowly, through natural processes or deliberate intervention. Stemming from this view and a whole-ecosystem perspective on forest succession, we offer management applications from this work and assess its broader relevance to understanding long-term change in other north temperate forest ecosystems.

54 ENVIRONMENTAL SCIENCES↗

AmeriFlux US-NC5 NC Butner Farm

This is the AmeriFlux version of the carbon flux data for the site US-NC5 NC Butner Farm. Site Description - The US-NC5 flux tower is located within an 80-year-old mixed pine-hardwood forest at the Umstead Research Farm in Butner, North Carolina. The northern section of this 20-hectare Fall Lake Watershed of the Neuse River Basin in the Piedmont of North Carolina, USA. The Northern portion is currently a managed cattle farm, which is slated for expansion—necessitating forest clearing in the flux site. To establish a reference baseline, a year-long, all-season eddy covariance flux monitoring campaign will be conducted from April 2025 to March 2026. This effort aims to capture the carbon flux dynamics of the mature forest ecosystem prior to a planned land-use conversion. The site will be transitioned into a silvopasture, maintained through prescribed burning and cattle grazing to promote an open-canopy watershed structure. Flux measurements will continue after the conversion.

Sun, Ge [USDA Forest Service]↗

AmeriFlux FLUXNET-1F US-NC5 NC Butner Farm

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-NC5 NC Butner Farm. This is the FLUXNET version of the carbon flux data for the site US-NC5 NC Butner Farm produced by applying the standard ONEFlux (1F) software. Site Description - The US-NC5 flux tower is located within an 80-year-old mixed pine-hardwood forest at the Umstead Research Farm in Butner, North Carolina. The northern section of this 20-hectare Fall Lake Watershed of the Neuse River Basin in the Piedmont of North Carolina, USA. The Northern portion is currently a managed cattle farm, which is slated for expansion—necessitating forest clearing in the flux site. To establish a reference baseline, a year-long, all-season eddy covariance flux monitoring campaign will be conducted from April 2025 to March 2026. This effort aims to capture the carbon flux dynamics of the mature forest ecosystem prior to a planned land-use conversion. The site will be transitioned into a silvopasture, maintained through prescribed burning and cattle grazing to promote an open-canopy watershed structure. Flux measurements will continue after the conversion.

Sun, Ge [USDA Forest Service]↗

Integrate FARM with PID controllers: IES Simulation Ecosystem Control System Development

The FARM (Feasible Actuator Range Modifier) software module is a component of the RAVEN-based FORCE framework for analysis of Integrated Energy Systems (IES). FARM was designed to support the HERON software module in the solution of the optimal dispatch problem for IES units. As the result of HERON-FARM dispatch simulation, the set-point trajectories are optimized to meet constraints on both the production variables (i.e., the variables to be optimized such as the electrical power, the hydrogen production rate, etc.) and the process variables tied to the service life of equipment (e.g., steam flowrate, vessel pressure, turbine firing temperature, etc.) at a coarse time resolution (every 10 or 100 seconds) over long time horizons (several days or weeks). In case the operational constraints need to be met at finer time resolution, the computational burden of HERON-FARM would linearly increase with the sampling rate, and sub-optimal solutions might be obtained. System responses characterized by overshoots and damped oscillations temporarily violating the imposed constraints might occur during abrupt power transients. In this report, a hierarchical control system architecture for the operation of the Dynamic Energy Technology and Integration Laboratory (DETAIL) facility constructed at INL was proposed. First, the preliminary studies on the proposed control strategy for operating the facility and the designed PI controllers were reviewed. In particular, the current approach for generating the set-point trajectories was studied, and its limits were identified. To this aim, the inclusion of a Supervisory Control layer embedding a modified version of the FARM algorithm for preserving the system safe operation over both long and real-time horizons was proposed. In this way, FARM would be applied twice, i.e., the original version (“FARM Validator”) aiding the solution of the power dispatch problem, and the modified version (“FARM Supervisory” coordinating the PI controllers to address the real-time control tasks. Despite the kernel of the two modules is the same algorithm, their roles, tasks, and capabilities are quite different. A detailed description of the role of FARM at addressing low-level control tasks is provided, along with tentative operational procedures for training the models embedded into the algorithm by using the collected experimental data.

42 ENGINEERING↗

The Design and Evaluation of Zero Trust Architecture for Electric Vehicle Charging Infrastructure: EVs @ Scale Series on EV Charging Station Cybersecurity

Implementing a zero trust architecture can significantly bolster the security of electric vehicle (EV) charging infrastructure. EV charging infrastructure includes numerous networked interfaces, each of which can present potential vulnerabilities. When these vulnerabilities are exploited, they can compromise the entire system, leading to severe operational and security risks. Zero trust is a security model that operates on the principle of "never trust, always verify," which helps manage the attack surface and limit the scope of any potential compromises. Fundamentally, this model ensures that no entity, whether inside or outside the network, is trusted by default. The design principles of zero trust include continuous verification, strict deny-by-default access controls, and micro-segmentation. Continuous verification ensures that every request is thoroughly checked, regardless of its origin. Strict access controls enforce the principle of least privilege, allowing users and devices only the minimum necessary access to perform their functions. Micro-segmentation involves dividing the network into smaller, isolated segments to prevent lateral movement in case of a breach. In the context of EV charging infrastructure, zero trust can be implemented through various strategies. For example, multi-factor authentication (MFA) can be required for engineers to access the management interfaces and control systems of charging stations. Real-time monitoring and analysis of network traffic can help detect and respond to anomalies. Systems that do not need to communicate with each other can be micro-segmented to enhance security. All communications should adhere to predefined policies to be permitted. Additionally, encrypting communications can protect sensitive information exchanged between chargers and management systems. This paper presents a zero trust architecture specifically designed for EV charging infrastructure. Implementing zero trust not only mitigates risks but also builds a resilient infrastructure capable of withstanding and quickly recovering from cyber threats. The architecture addresses six defined security objectives. A comprehensive test plan is developed to assess the architecture against these objectives, and the results of the evaluation are reported. This approach is essential for maintaining the reliability and integrity of EV charging services in an increasingly interconnected and vulnerable digital landscape. This is the first in a planned series of papers exploring the implementation of zero trust in EV charging infrastructure. Each paper will delve into different aspects and applications of zero trust, highlighting how various work processes and requirements can lead to distinct architectural designs. These architectures will be tailored to address specific security challenges and operational needs within the EV charging ecosystem, ensuring a robust and adaptable security framework.

33 ADVANCED PROPULSION SYSTEMS↗

The LCLStream Ecosystem for Multi-Institutional Dataset Exploration

We describe a new end-to-end experimental data streaming framework designed from the ground up to support new types of applications – AI training, extremely high-rate X-ray time-of-flight analysis, crystal structure determination with distributed processing, and custom data science applications and visualizers yet to be created. Throughout, we use design choices merging cloud microservices with traditional HPC batch execution models for security and flexibility. This project makes a unique contribution to the DOE Integrated Research Infrastructure (IRI) landscape. By creating a flexible, API-driven data request service, we address a significant need for high-speed data streaming sources for the X-ray science data analysis community. With the combination of data request API, mutual authentication web security framework, job queue system, high-rate data buffer, and complementary nature to facility infrastructure, the LCLStreamer framework has prototyped and implemented several new paradigms critical for future generation experiments.

Rogers, David [ORNL] (ORCID:0000000251871768)↗

COMPASS-FME Terrestrial Ecosystem Manipulation to Probe the Effects of Storm Treatments (TEMPEST) Experiment Level 2 Sensor Data v2-1

This is the version v2-1 Level 2 (L2) data release for COMPASS-FME environmental sensors located at our Terrestrial Ecosystem Manipulation to Probe the Effects of Storm Treatments (TEMPEST) experimental site. This manipulative, ecosystem-scale TEMPEST experiment addresses the potential for freshwater and estuarine-water disturbance events to alter tree function, species composition, and ecosystem processes in a deciduous coastal forest in MD, USA. The experiment uses a large-unit (2000 m2), un-replicated experimental design, with three 50 m × 40 m plots serving as control, freshwater, and estuarine-water treatments. Level 2 (L2) data consist of sensor observations from the COMPASS-FME synoptic sites, TEMPEST, and DELUGE. Compared to the L1 data, these are more consistent (always 15-minute timestamps for the entire year); better QA/QC’d (out of bounds, out of service, and extreme outlier values are removed); and more complete, with a gap-filled time series available alongside the main observations, and additional derived (calculated) variables. L2 data are intended to be rapidly and easily usable in analyses and simulations. However, algorithmic outlier identification always carries the risk of removing valid data, and Level 1 data may be more suitable for analyses that focus on variability or extreme events. This dataset includes: - An overall dataset README file that describes the current version, gives citation and contact information, etc. - Site- and year-specific folders, each holding variable-specific Parquet (a high performance, space efficient format; see https://parquet.apache.org) data files for each site and plot in that year. - Metadata files within each site-year folder provide full information on data units, expected ranges, contact information, detailed flood times, as well as a general description of the site. - Environmental sensor types that appear in the data files include weather (ClimaVUE50, CS, RM Young, and LI instruments in the graphs below); soil conditions (TEROS12); soil redox state (Redox); groundwater variables (AquaTROLL200 and AquaTROLL600); open water sondes (Exo); tree sap velocity (Sapflow); and system voltage and state (Datalogger). Data are reported every 15 minutes. Please see v2-1 TEMPEST L2 Sensor Package Quick Start.pdf for detailed information on data package structure, temporal coverage, and versioning. Data files are in Apache Parquet, a high performance, space efficient format for tabular data. These files can be read using R's `arrow` package (https://arrow.apache.org/docs/r/), with similar tools available in other languages. The TEMPEST flood events occurred on the following dates. They lasted for ~10 hours each day and delivered ~80,000 gallons to each plot; many data streams are available at 1 or 5 minute frequency during these periods. * Tests: Aug 25 (fresh plot) and Sep 9 (salt plot), 2021 * TEMPEST 1: June 22, 2022 * TEMPEST 2: June 6-7, 2023 * TEMPEST 3: June 11-13, 2024

EARTH SCIENCE > ATMOSPHERE > ATMOSPHERIC TEMPERATU↗

Food web dynamics drive variation in Smallmouth Bass Micropterus dolomieu mercury contamination in protected Southern Appalachian streams

Mercury is a global pollutant that threatens otherwise protected aquatic ecosystems. Mercury food web dynamics are not well understood in streams with no point sources of contamination but have the potential to concentrate this dangerous pollutant in upper trophic level consumers. A 2016 study revealed that mercury concentrations of Smallmouth Bass Micropterus dolomieu varied between three streams in Great Smoky Mountains National Park (GSMNP), Tennessee, USA. We tested the hypothesis that food web interactions drive spatial variation in mercury concentrations of the apex predator of protected stream ecosystems. We measured carbon and nitrogen stable isotope ratios, and mercury concentrations of eight food web components of GSMNP streams including basal resources, intermediate consumers, and Smallmouth Bass, the apex predator. Mean THg concentrations of basal resources did not differ between streams, but the spatial pattern of concentrations of intermediate consumers mirrored Smallmouth Bass. Relationships between organismal contaminant concentrations and trophic level were positive in all three streams, indicating biomagnification is occurring in the protected streams of GSMNP. Furthermore, our findings indicate that mercury dynamics in intermediate trophic levels, rather than differences in basal resource concentrations, may drive differences in mercury contamination of apex predators of the protected stream ecosystems of GSMNP.

Bioaccumulation↗

Getting lost tracking the carbon footprint of hydropower

In the transition to low-carbon electricity, well-quantified estimates of carbon dynamics are needed to ensure that emissions reduction targets are achieved. Here we review the state of the science on carbon accounting for hydropower reservoirs and identify limitations and future solutions. Nearly all research on reservoir greenhouse-gas (GHG) emissions has focused on individual reservoirs in isolation without considering their position in a freshwater network draining organic matter from upstream watersheds or the coordinated operation of reservoir cascades. Second, carbon inventories have extrapolated from a small, non-probabilistic sample of highly variable measurements of GHG emissions to unsampled reservoirs. A stronger statistical foundation is needed to estimate a global inventory and its uncertainty. Third, attribution to hydropower is based on ranks assigned to reservoir purpose. Instead, the physical influence of hydropower on carbon dynamics could be directly measured. Fourth, current carbon-accounting practices neglect time. A time-varying approach would quantify variation in emissions for electricity portfolios from changes in the fuel mix at different times and account for ancillary services, i.e., the ability to support the grid when variable renewables are not available without using natural gas. Reservoirs also sequester a significant portion of inflowing carbon in sediments and slow the carbon cycle by delaying the return of carbon to the atmosphere for decades to centuries. Together, these refinements would help to illuminate pathways toward meeting energy demand with the longest-possible delay in returning carbon to the atmosphere and without adding ancient sources to the pool of carbon cycling through aquatic ecosystems.

13 HYDRO ENERGY↗

SolarPlus Optimizer: Integrated Control of Solar, Batteries, and Flexible Loads for Small Commercial Buildings

Building-level microgrids may be a key strategy to unlock the combined potential of flexible loads, renewable generation, and energy storage. However, few software options exist for integrated control of building loads and other distributed energy resources at this scale. The commercial software solutions on the market can force customers to adopt one particular ecosystem of products, thus limiting consumer choice. The SolarPlus Optimizer (SPO) is an open-source building-level microgrid control platform that uses Model Predictive Control to optimize both building loads and behind-the-meter energy storage to reduce energy bills and increase demand flexibility. This paper evaluates the capabilities of SPO in a small commercial building in Northern California under multiple electricity tariffs and demand response scenarios. Comparing SPO operation with an emulated battery and baseline operation employing a commercial optimization service, SPO reduced electricity bills by an estimated 7.3% in summer, 3.2% in spring, and 3.7% in winter. In a “load shape” scenario meant to counter the “duck curve”, SPO achieved 71% fewer violations from the load signal than the baseline control method. During a three hour long load shed event, SPO reduced cooling and refrigeration load by 38%. This research shows significant potential to provide load flexibility for building-level microgrids for this type of control systems. Finally, the paper discusses the future direction of research on open-source control systems.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

A haplotype‐resolved reference genome of Quercus alba sheds light on the evolutionary history of oaks

Summary White oak ( Quercus alba ) is an abundant forest tree species across eastern North America that is ecologically, culturally, and economically important. We report the first haplotype‐resolved chromosome‐scale genome assembly of Q. alba and conduct comparative analyses of genome structure and gene content against other published Fagaceae genomes. We investigate the genetic diversity of this widespread species and the phylogenetic relationships among oaks using whole genome data. Despite strongly conserved chromosome synteny and genome size across Quercus , certain gene families have undergone rapid changes in size, including defense genes. Unbiased annotation of resistance (R) genes across oaks revealed that the overall number of R genes is similar across species – as are the chromosomal locations of R gene clusters – but, gene number within clusters is more labile. We found that Q. alba has high genetic diversity, much of which predates its divergence from other oaks and likely impacts divergence time estimations. Our phylogenetic results highlight widespread phylogenetic discordance across the genus. The white oak genome represents a major new resource for studying genome diversity and evolution in Quercus . Additionally, we show that unbiased gene annotation is key to accurately assessing R gene evolution in Quercus .

Larson, Drew A. [Department of Biology Indiana Uni↗

PAVC Gridded 20m Alaska NGEE Tier3 PFTs v1.0

These 20-meter spatial resolution gridded products provide per-pixel fractional cover (%) of Next Generation Ecosystem Experiments (NGEE) Arctic Plant Functional Types (PFTs) Tier 3 across Alaska, north of the boreal treeline. The products were developed for the NGEE Arctic project, which is improving Arctic vegetation representation and parameterization of the E3SM Land Model. This dataset includes 8 files containing fractional cover for NGEE Tier 3 PFTs (https://data.ess-dive.lbl.gov/view/doi:10.15485/2529470): (1) bryophytes; (2) lichens; (3) non-vascular plants, i.e., the sum of lichens and bryophytes; (4) deciduous shrubs, (5) evergreen shrubs, (6) forbs, (7) graminoids, and a non-PFT class, (8) litter. Each pixel contains the percent cover (expressed as a fraction of total ground cover) that was predicted by random-forest regression models. The random-forest models were trained on cover data collected at 978 plots from 2010 to 2021, of which are archived in the Pan-Arctic Vegetation Cover (PAVC) database (https://data.ess-dive.lbl.gov/datasets/doi:10.15485/2483557). The plot cover was linked to 20-meter spatial resolution, satellite-derived predictor variables: Sentinel-2 spectra and Sentinel-1 polarizations averaged over the 2019 growing season, as well as topographical features derived from ArcticDEM. Then, spatio-temporally anomalous plot data that introduced large variability to the regression outcomes were dropped using the Cook’s distance outlier detection method, and the models were re-created using high-quality plots and their associated satellite derived explanatory variables per each PFT. The correlations between plot-observed and satellite-derived fractional cover for all PFTs were well correlated (R2 = 0.69–0.95 and 0.5 for litter) and had low RMSE bias (0.02–0.11). This research was performed as a part of the NGEE Arctic project. The NGEE Arctic project was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.

54 ENVIRONMENTAL SCIENCES↗

Next generation Arctic vegetation maps: Aboveground plant biomass and woody dominance mapped at 30 m resolution across the tundra biome

The Arctic is warming faster than anywhere else on Earth, placing tundra ecosystems at the forefront of global climate change. Plant biomass is a fundamental ecosystem attribute that is sensitive to changes in climate, closely tied to ecological function, and crucial for constraining ecosystem carbon dynamics. However, the amount, functional composition, and distribution of plant biomass are only coarsely quantified across the Arctic. Therefore, we developed the first moderate resolution (30 m) maps of live aboveground plant biomass (g m −2 ) and woody plant dominance (%) for the Arctic tundra biome, including the mountainous Oro Arctic. We modeled biomass for the year 2020 using a new synthesis dataset of field biomass harvest measurements, Landsat satellite seasonal synthetic composites, ancillary geospatial data, and machine learning models. Additionally, we quantified pixel-wise uncertainty in biomass predictions using Monte Carlo simulations and validated the models using a robust, spatially blocked and nested cross-validation procedure. Observed plant and woody plant biomass values ranged from 0 to ∼6000 g m −2 (mean ≈ 350 g m −2 ), while predicted values ranged from 0 to ∼4000 g m −2 (mean ≈ 275 g m −2 ), resulting in model validation root-mean-squared-error (RMSE) ≈ 400 g m −2 and R 2 ≈ 0.6. Our maps not only capture large-scale patterns of plant biomass and woody plant dominance across the Arctic that are linked to climatic variation (e.g., thawing degree days), but also illustrate how fine-scale patterns are shaped by local surface hydrology, topography, and past disturbance. By providing data on plant biomass across Arctic tundra ecosystems at the highest resolution to date, our maps can significantly advance research and inform decision-making on topics ranging from Arctic vegetation monitoring and wildlife conservation to carbon accounting and land surface modeling.

Climate change↗

Burrowing Owl Survey Report for Sandia National Laboratories: 2025

The Burrowing Owl Survey Report for Sandia National Laboratories: 2025 provides data and analysis on occupancy surveys that were conducted during the 2025 survey season. The surveys were conducted in partnership with Kirtland Air Force Base’s Natural Resource Program and the United States Fish and Wildlife Service to maintain and expand on the long-term dataset on burrowing owls on base. Ecology Program biologists conducted roadside census surveys at survey areas previously identified by Kirtland Air Force Base along with several additional survey areas deemed suitable for occupancy. Remote sensor camera monitoring was also conducted on three nest burrows. In total, 12 breeding pairs of burrowing owls were confirmed in 2025. Several owls were confirmed to have leg bands, indicating previous captures and recurrence of these specific individuals on base. Occupancy surveys on burrowing owls continue to provide valuable information on ecosystem health and can serve to inform future management direction and decisions.

54 ENVIRONMENTAL SCIENCES↗

Distributed Resources for the Earth System Grid Federation (ESGF) Advanced Management (DREAM). Final Report

Distributed Resources for the Earth System Grid Federation (ESGF) Advanced Management (DREAM) is a proposed system that will enable data from an infinite number of diverse sources to be organized and accessed from anywhere using any handheld or other computer device. The approach offers a powerful roadmap for the creation and integration of a unified knowledge base of an entire ecosystem, including its many geophysical, geographical, social, political, agricultural, energy, transportation, and cyber aspects. The resulting aggregation of data has the potential to generate an informational universe of unprecedented size that has never before been possible due to the prohibitive costs, managerial complexity, and technical barriers associated with ever-changing exponential-growth data flows. We envision that DREAM will accelerate discovery by enabling climate researchers, among other types of researchers, to manage, analyze, and visualize data from earth-scale measurements and simulations. DREAM’s success will be built on proven components that leverage existing services and resources. A key building block for DREAM will be the ESGF, chaired by Dean N. Williams. Expanding on the existing ESGF, the project will ensure that the access, storage, movement, and analysis of the large quantities of data that are processed and produced by diverse science projects can be dynamically distributed with proper resource management. Much of the Office of Science data is currently generated by multiple stand-alone facilities. DREAM can collect data accumulated from these facilities and incorporate it into a fully integrated network accessible from anywhere in the world. The result is a completely new paradigm shift for data management, analysis, and visualization enabling researchers to: Manage their calculations, data, tools, and research results; Ensure that all data are sharable, reproducible and (re)usable—accompanied by appropriate metadata describing its provenance, syntax, and semantics at creation; Advance application performance by selectively adapting APIs and services in response to scientific requirements and architectural complexities; and Provide scalable interactive resource management—navigate data and metadata at multiple levels, provide architecture-aware data integration, analysis and visualization tools. We will engage closely with DOE, NASA, and NOAA science groups working at the leading edge of computing. These engagements—in domains such as biology, climate, and hydrology—will allow us to advance disciplinary science goals and inform our development of technologies that can accelerate discovery across DOE more broadly. We will advertise and promote our technologies via dedicated workshops, tutorials, and sessions at conferences, stand-alone events with broad inter-disciplinary invitation, and engagements with leadership facilities.

54 ENVIRONMENTAL SCIENCES↗