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At least 325 records · Page 18

Modular Pulsed Plasma Electric Propulsion System for Cubesats

Current capabilities of CubeSats must be improved in order to perform more ambitious missions. Electric propulsion systems will play a key role due to their large specific impulse. Compared to other propulsion alternatives, their simplicity allows an easier miniaturization and manufacturing of autonomous modules into the nano and pico-satellite platform. Pulsed Plasma Thrusters (PPTs) appear as one of the most promising technologies for the near term. The utilization of solid and non-volatile propellants, their low power requirements and their proven reliability in the large scale make them great candidates for rapid implementation. The main challenges are the integration and miniaturization of all the electronic circuitry into a printed circuit board (PCB) that can satisfy the strict requirements that CubeSats present. NASA Ames and the George Washington University have demonstrated functionality and control of three discrete Micro-Cathode Arc Thrusters (CAT) using a bench top configuration that was compatible with the ARC PhoneSat Bus. This demonstration was successfully conducted in a vaccum chamber at the ARC Environmental Test Laboratory. A new effort will integrate a low power Plasma Processing Unit and two plasma thrusters onto a single printed circuit board that will utilize less than 13 U of Bus volume. The target design will be optimized for the accommodation into the PhoneSatEDISON Demonstration of SmallSatellite Networks (EDSN) bus as it uses the same software interface application, which was demonstrated in the previous task. This paper describes the design, integration and architecture of the proposed propulsion subsystem for a planned Technology Demonstration Mission. In addition, a general review of the Pulsed Plasma technology available for CubeSats is presented in order to assess the necessary challenges to overcome further development.

PCB↗

Developing Model Benchtop Systems for Microbial Experimental Evolution

Understanding how microbes impact an ecosystem has improved through advances of molecular and genetic tools, but creating complex systems that emulate natural biology goes beyond current technology. In fact, many chemical, biological, and metabolic pathways of even model organisms are still poorly characterized. Even then, standard laboratory techniques for testing microbial impact on environmental change can have many drawbacks; they are time-consuming, labor intensive, and are at risk of contamination. By having an automated process, many of these problems can be reduced or even eliminated. We are developing a benchtop system that can run for long periods of time without the need for human intervention, involve multiple environmental stressors at once, perform real-time adjustments of stressor exposure based on current state of the population, and minimize contamination risks. Our prototype device allows operators to generate an analogue of real world micro-scale ecosystems that can be used to model the effects of disruptive environmental change on microbial ecosystems. It comprises of electronics, mechatronics, and fluidics based systems to control, measure, and evaluate the before and after state of microbial cultures from exposure to environmental stressors. Currently, it uses four parallel growth chambers to perform tests on liquid cultures. To measure the population state, optical sensors (LED/photodiode) are used. Its primary selection pressure is UV-C radiation, a well-studied stressor known for its cell- and DNA-damaging effects and as a mutagen. Future work will involve improving the current growth chambers, as well as implementing additional sensors and environmental stressors into the system. Full integration of multiple culture testing will allow inter-culture comparisons. Besides the temperature and OD sensors, other types of sensors can be integrated such as conductivity, biomass, pH, and dissolved gasses such as CO and O. Additional environmental stressor systems like temperature (extreme heat or cold), metal toxicity, and other forms of radiation will increase the scale and testing range.

Developing↗

Methylation Pattern Detection in the Genome of Bacillus Pumilus Strain SAFR-032

Bacillus pumilus SAFR-032, an endospore-forming bacterial strain that was isolated from a spacecraft assembly facility (SAFR), was investigated to determine its methylation pattern (methylome) across the genome in comparison to the previously sequenced reference genome. In addition, a version of SAFR-032 that was flown as spores for 18 months on the International Space Station (ISS) was also investigated for possible genomic changes due to long-duration ISS-flight and to determine if methylation patterns may have changed. Both the genomics and methylomics were conducted using a Nanopore MinION sequencing device. In addition to the omics investigation, the two SAFR-032 strains, ISS flown and non-ISS flown, were compared phenotypically in chamber experiments testing individual environmental insults: ionizing radiation, UV exposure, and cold desiccation (i.e. freeze drying). Results from this study inform on Planetary Protection concerns and will reveal potential DNA damage associated with long-term spaceflight and how such damage may influence survivors after being transported to an extraterrestrial environment, such as Mars.

Serda, Bianca M.↗

Initial dynamic photoactive materials testing of an atmospheric chamber intended for radioactive and hazardous gases

Radioisotopes and hazardous gases can have undetermined environmental pathways. Researchers at Pacific Northwest National Laboratory constructed a chamber that complies with the requirements needed for an atmospheric reaction platform and the safety principles of interacting with hazardous dispersible sources to enable the environmental testing of these gases. Initial dynamic testing showed inter-chamber mixing completed from minutes to 1.5 hours. The photooxidation of butyl iodine showed the presence of signals from reaction products, and intermediaries for up to 50 hours. Current detection limits of the chamber and analytical collection and testing approach were shown to be in the single-digit parts per billion levels. In conclusion, the comparisons between the measured oxidation trends and literature show the utility of performing laboratory experiments to validate the results of modeling for larger-scale scenarios.

54 ENVIRONMENTAL SCIENCES↗

Multiple test chamber exposes materials to various environments

Multiple compartment test chamber exposes several material specimens to various environmental conditions for prolonged periods. The specimens are individually mounted in chamber compartments, rotated to various positions, and measured through optical windows to determine progressive changes in the material properties.

Johnston, R. L.↗

Space station protective coating development

A generic list of Space Station surfaces and candidate material types is provided. Environmental exposures and performance requirements for the different Space Station surfaces are listed. Coating materials and the processing required to produce a viable system, and appropriate environmental simulation test facilities are being developed. Mass loss data from the original version of the atomic oxygen test chamber and the improved facility; additional environmental exposures performed on candidate materials; and materials properties measurements on candidate coatings to determine the effects of the exposures are discussed. Methodologies of production, and coating materials, used to produce the large scale demonstration articles are described. The electronic data base developed for the contract is also described. The test chamber to be used for exposure of materials to atomic oxygen was built.

Pippin, H. G.↗

Instrumentation for Examining Microbial Response to Changes In Environmental Pressures

The Automated Adaptive Directed Evolution Chamber (AADEC) is a device that allows operators to generate a micro-scale analog of real world systems that can be used to model the local-scale effects of climate change on microbial ecosystems. The AADEC uses an artificial environment to expose cultures of micro-organisms to environmental pressures, such as UV-C radiation, chemical toxins, and temperature. The AADEC autonomously exposes micro-organisms to selection pressures. This improves upon standard manual laboratory techniques: the process can take place over a longer period of time, involve more stressors, implement real-time adjustments based on the state of the population, and minimize the risk of contamination. We currently use UV-C radiation as the main selection pressure, UV-C is well studied both for its cell and DNA damaging effects as a type of selection pressure and for its related effectiveness as a mutagen; having these functions united makes it a good choice for a proof of concept. The AADEC roadmap includes expansion to different selection pressures, including heavy metal toxicity, temperature, and other forms of radiation.The AADEC uses closed-loop control to feedback the current state of the culture to the AADEC controller that modifies selection pressure intensity during experimentation, in this case culture density and growth rate. Culture density and growth rate are determined by measuring the optical density of the culture using 600 nm light. An array of 600 nm LEDs illuminate the culture and photodiodes are used to measure the shadow on the opposite side of the chamber.Previous experiments showed that we can produce a million fold increase to UV-C radiation over seven iterations. The most recent implements a microfluidic system that can expose cultures to multiple different selection pressures, perform non-survival based selection, and autonomously perform hundreds of exposure cycles. A scalable pump system gives the ability to pump in various different growth media to individual cultures and introduce chemical toxins during experimentation; AADEC can perform freeze and thaw cycles. We improved our baseline characterization by building a custom UV-C exposure hood, a shutter operates on a preset timer allowing the user to set exposure intensity consistently for multiple iterations.

Environment↗

Zero-gravity cloud physics laboratory: Candidate experiments definition and preliminary concept studies

The candidate definition studies on the zero-g cloud physics laboratory are covered. This laboratory will be an independent self-contained shuttle sortie payload. Several critical technology areas have been identified and studied to assure proper consideration in terms of engineering requirements for the final design. Areas include chambers, gas and particle generators, environmental controls, motion controls, change controls, observational techniques, and composition controls. This unique laboratory will allow studies to be performed without mechanical, aerodynamics, electrical, or other type techniques to support the object under study. This report also covers the candidate experiment definitions, chambers and experiment classes, laboratory concepts and plans, special supporting studies, early flight opportunities and payload planning data for overall shuttle payload requirements assessments.

Eaton, L. R.↗

SPRUCE: Carbon Dioxide and Methane Soil Flux Measurements at High Temporal Resolution, Beginning in 2022

This data set contains high temporal resolution measurements of greenhouse gas flux (CO2 and CH4) from the SPRUCE (Spruce and Peatland Responses Under Changing Environments) experiment (Hanson et al. 2017) over the growing seasons in 2022 (30-minute resolution) and 2023 (15-minute resolution). Additional data will be appended to this dataset periodically until the close out of the SPRUCE experiment in 2025. This dataset contains two data files in comma-separate (*.csv) format. ABB GLA131-GGA CO2/CH4 gas analyzers, each paired with an Eosense eosMX multiplexer and 2 eosAC-LT automated flux chambers, were deployed from during the 2022 and 2023 growing seasons (2022-06-06 – 2023-10-26). Each SPRUCE experimental enclosure included two Eosense 50 cm diameter flux chambers with 40 cm tall stainless steel collars embedded approximately 15 cm into the peat soil, to measure soil gas flux of peatland ecosystem moss-shrub communities. . Measurements were taken for three minutes, alternating between the flux chambers, with each Eosense flux chamber sampling gas fluxes four times per hour in 2023 and twice per hour for 2022. In addition, time-series environmental data measured at the flux chamber are included: Photosynthetically active radiation (PAR), soil temperature, soil volumetric water content (at 10 cm depth), air temperature, soil temperature (0-200 cm depth), and volumetric water at 20 cm depth.

Spruce and Peatland Responses Under Changing Envir↗

Vegetation Warming Experiment: 15N Uptake Experiment Environmental Observations and Thaw Depth, Utqiagvik (Barrow), Alaska, 2018

This dataset consists of measured soil thaw depth, soil temperature, soil moisture, and Arctagrostis latifolia height in vegetation warming experiment enclosures and paired control plots. Vegetation warming chambers (Zero Power) were deployed on the Barrow Environmental Observatory (BEO), Utqiagvik, Alaska. These chambers (Figure 1) consistently elevated air temperatures by approximately 4°C using a self-venting system described by Lewin et al (2017). Five chambers were deployed from June 17, 2018 to September 24, 2018 on the BEO within a 1 km2 area centered on 71.275N, -156.641W. Each chamber was co-located with an ambient plot where temperatures were not manipulated on patches of tundra containing the target species Arctagrostis latifolia. An intensive field campaign in late July investigated the impact of warming had on A. latifolia biomass, chemistry, and uptake of 15N labeled ammonia that was injected into the surface soils for one week. Initial measurements were taken on July 21, 2018. Harvest occurred on July 27, 2018. Water-extractable nutrients in soils were measured in July following harvests of A. latifolia plants and underlying soils. Availability of ammonia, nitrate, and phosphate throughout the growing season was measured by extracting nutrients bound to anion and cation binding resins deployed from July through September. Environmental variables (thaw depth, surface soil temperatures, surface soil moisture) were measured. Leaf traits and root traits of A. latifolia were also measured. Included are two *.csv data files and one *.pdf user guide. The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research. The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska. Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

ML-based calibration and control of the GlueX Central Drift Chamber

The GlueX Central Drift Chamber (CDC) in Hall D at Jefferson Lab, used for detecting and tracking charged particles, is calibrated and controlled during data taking using a Gaussian process. The system dynamically adjusts the high voltage applied to the anode wires inside the chamber in response to changing environmental and experimental conditions such that the gain is stabilized. Control policies have been established to manage the CDC's behavior. These policies are activated when the model's uncertainty exceeds a configurable threshold or during human-initiated tests during normal production running. Finally, we demonstrate the system reduces the time detector experts dedicate to calibration of the data offline, leading to a marked decrease in computing resource usage without compromising detector performance.

47 OTHER INSTRUMENTATION↗

Soil surface CO2 fluxes and the carbon budget of a grassland

Measurements of soil surface CO2 fluxes are reported for three sites within the First International Satellite Land Surface Climatology Project (ISLSCP) Field Experiment (FIFE) area, and simple empirical equations are fit to the data to provide predictions of soil fluxes from environmental observations. A prototype soil chamber, used to make the flux measurements, is described and tested by comparing CO2 flux measurements to a 40-L chamber, a 1-m/cu chamber, and eddy correlation. Results suggest that flux measurements with the prototype chamber are consistent with measurements by other methods to within about 20 percent. A simple empirical equation based on 10-cm soil temperature, 0- to 10-cm soil volumetric water content, and leaf area index predicts the soil surface CO2 flux with a rms error of 1.2 micro-mol sq m/s for all three sites. Further evidence supports using this equation to evaluate soil surface CO2 during the 1987 FIFE experiment. The soil surface CO2 fluxes when averaged over 24 hours are comparable to daily gross canopy photosynthetic rates. For 6 days of data the net daily accumulation of carbon is about 0.6 g CO2 sq m/d; this is only a few percent of the daily gross accumulation of carbon by photosynthesis. As the soil became drier in 1989, the net accumulation of carbon by the prairie increased, suggesting that the soil flux is more sensitive to temperature and drought than the photosynthetic fluxes.

Norman, J. M.↗

Vegetation Warming Experiment: Thaw depth and dGPS locations, Utqiagvik, Alaska, 2019

Thaw depth measurements within and around warming chambers, and in ambient plots located on the Barrow Environmental Observatory (BEO), Utqiagvik, Alaska. Measurements were taken at the start and end of chamber deployment, and two intermediate times during the 2019 growth season. dGPS measurements of chamber and ambient plot locations are also included. The files included in this data package are in .csv format, and include 3 data files and 3 metadata files. This data was recorded as part of the Zero Power Warming (ZPW) vegetation warming experiment. See related data files for environmental conditions, leaf physiology, leaf traits, and landscape and plot phenocam images. The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research. The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska. Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

Vegetation Warming Experiment: Thaw depth and dGPS locations, Utqiagvik, Alaska, 2021

Thaw depth measurements within and around warming chambers, and in ambient plots located on the Barrow Environmental Observatory (BEO), Utqiagvik, Alaska. Measurements were taken at the start and end of chamber deployment, and two intermediate times during the 2021 growth season. dGPS measurements of chamber and ambient plot locations are also included. The files included in this data package are in .csv format, and include 2 data files and 3 metadata files. This data was recorded as part of the Zero Power Warming (ZPW) vegetation warming experiment. Other datasets under the Vegetation Warming Experiment include data for environmental conditions, leaf physiology, leaf traits, and landscape and plot phenocam images. The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research. The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska. Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

Scaling of Turbulence and Microphysics in a Convection–Cloud Chamber of Varying Height

Abstract The convection–cloud chamber enables measurement of aerosol and cloud microphysics, as well as their interactions, within a turbulent environment under steady‐state conditions. Increasing the size of a convection–cloud chamber, while holding the imposed temperature difference constant, leads to increased Rayleigh, Reynolds and Nusselt numbers. Large–eddy simulation coupled with a bin microphysics model allows the influence of increased velocity, time, and spatial scales on cloud microphysical properties to be explored. Simulations of a convection–cloud chamber, with fixed aspect ratio and increasing heights of H = 1, 2, 4, and (for dry conditions only) 8 m are performed. The key findings are: Velocity fluctuations scale as H 1/3 , consistent with the Deardorff expression for convective velocity, and implying that the turbulence correlation time scales as H 2/3 . Temperature and other scalar fluctuations scale as H −3/7 . Droplet size distributions from chambers of different sizes can be matched by adjusting the total aerosol injection rate as the horizontal cross‐sectional area (i.e., as H 2 for constant aspect ratio). Injection of aerosols at a point versus distributed throughout the volume makes no difference for polluted conditions, but can lead to cloud droplet size distribution broadening in clean conditions. Cloud droplet growth by collision and coalescence leads to a broader right tail of the distribution compared to condensation growth alone, and this tail increases in magnitude and extent monotonically as the increase of chamber height. These results also have implications for scaling within turbulent, cloudy mixed‐layers in the atmosphere, such as fog layers.

54 ENVIRONMENTAL SCIENCES↗

Expert System Control of Plant Growth in an Enclosed Space

The Expert System is an enclosed, controlled environment for growing plants, which incorporates a computerized, knowledge-based software program that is designed to capture the knowledge, experience, and problem-solving skills of one or more human experts in a particular discipline. The Expert System is trained to analyze crop/plant status, to monitor the condition of the plants and the environment, and to adjust operational parameters to optimize the plant-growth process. This system is intended to provide a way to remotely control plant growth with little or no human intervention. More specifically, the term control implies an autonomous method for detecting plant states such as health (biomass) or stress and then for recommending and implementing cultivation and/or remediation to optimize plant growth and to minimize consumption of energy and nutrients. Because of difficulties associated with delivering energy and nutrients remotely, a key feature of this Expert System is its ability to minimize this effort and to achieve optimum growth while taking into account the diverse range of environmental considerations that exist in an enclosed environment. The plant-growth environment for the Expert System could be made from a variety of structures, including a greenhouse, an underground cavern, or another enclosed chamber. Imaging equipment positioned within or around the chamber provides spatially distributed crop/plant-growth information. Sensors mounted in the chamber provide data and information pertaining to environmental conditions that could affect plant development. Lamps in the growth environment structure supply illumination, and other additional equipment in the chamber supplies essential nutrients and chemicals.

May, George↗

Glaciation of mixed-phase clouds: insights from bulk model and bin-microphysics large-eddy simulation informed by laboratory experiment

Abstract. Mixed-phase clouds affect precipitation and radiation differently from liquid and ice clouds, posing greater challenges to their representation in numerical simulations. Recent laboratory experiments using the Pi Cloud Chamber explored cloud glaciation conditions based on increased injection of ice-nucleating particles. In this study, we use two approaches to reproduce the results of the laboratory experiments: a bulk scalar mixing model and large-eddy simulation (LES) with bin microphysics. The first approach assumes a well-mixed domain to provide an efficient assessment of the mean cloud properties for a wide range of conditions. The second approach resolves the energy-carrying turbulence, the particle size distribution, and their spatial distribution to provide more details. These modeling approaches enable a separate and detailed examination of liquid and ice properties, which is challenging in the laboratory. Both approaches demonstrate that, with an increased ice number concentration, the flow and microphysical properties exhibit the same changes in trends. Additionally, both approaches show that the ice integral radius reaches the theoretical glaciation threshold when the cloud is subsaturated with respect to liquid water. The main difference between the results of the two approaches is that the bulk model allows for the complete glaciation of the cloud. However, LES reveals that, in a dynamic system, the cloud is not completely glaciated as liquid water droplets are continuously produced near the warm lower boundary and subsequently mixed into the chamber interior. These results highlight the importance of the ice mass fraction in distinguishing the mixed-phase clouds and ice clouds.

54 ENVIRONMENTAL SCIENCES↗

Specifications for and preliminary design of a plant growth chamber for orbital experimental experiments

It was proposed that plant experiments be performed on board the space shuttle. To permit the proper execution of most tests, the craft must contain a plant growth chamber which is adequately designed to control those environmental factors which can induce changes in a plant's physiology and morphology. The various needs of, and environmental factors affecting, plants are identified. The permissilbe design, construction and performance limits for a plant-growth chamber are set, and tentative designs were prepared for units which are compatible with both the botanical requirements and the constraints imposed by the space shuttle.

Sweet, H. C.↗