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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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NASA NTRS1993

Cytokines and immune surveillance in humans

Sonnenfeld, Gerald↗

NASA NTRS1993

The effects of video compression on acceptability of images for monitoring life sciences' experiments

Current plans indicate that there will be a large number of life science experiments carried out during the thirty year-long mission of the Biological Flight Research Laboratory (BFRL) on board Space Station Freedom (SSF). Non-human life science experiments will be performed in the BFRL. Two distinct types of activities have already been identified for this facility: (1) collect, store, distribute, analyze and manage engineering and science data from the Habitats, Glovebox and Centrifuge, (2) perform a broad range of remote science activities in the Glovebox and Habitat chambers in conjunction with the remotely located principal investigator (PI). These activities require extensive video coverage, viewing and/or recording and distribution to video displays on board SSF and to the ground. This paper concentrates mainly on the second type of activity. Each of the two BFRL habitat racks are designed to be configurable for either six rodent habitats per rack, four plant habitats per rack, or a combination of the above. Two video cameras will be installed in each habitat with a spare attachment for a third camera when needed. Therefore, a video system that can accommodate up to 12-18 camera inputs per habitat rack must be considered.

Haines, Richard F.↗

NASA NTRS1988

The pineal gland - Its possible roles in human reproduction

The paper discusses the role of the pineal gland in controlling mammalian reproduction, with particular attention given to the role of melatonin in polyestrus mammals, like humans and laboratory rodents. Evidence is cited indicating the influence of melatonin production and blood content on the age of puberty, the timing of the ovulatory cycle, gonadal steriodogenesis, and patterns of reproductive behavior. It is suggested that abnormal patterns of melatonin might be associated with amenorrhea, anovulation, unexplained infertility, premature menopause, and habitual abortions.

Brzezinski, Amnon↗

NASA NTRS1992

Dark cycle monitoring of biological specimens on Space Station Freedom

The operational environment for biological research on Space Station Freedom will incorporate video technology for monitoring plant and animal subjects. The video coverage must include dark-cycle monitoring because early experiments will use rodents that are nocturnal and therefore most active during the dark part of the daily cycle. Scientific requirements for monitoring during the dark cycle are exacting. Infrared (IR) or near-IR sensors are required. The trade-offs between these two types of sensors are based on engineering constraints, sensitivity spectra, and the quality of imagery possible from each type. This paper presents results of a study conducted by the Biological Flight Research Projects Office in conjunction with the Spacecraft Data Systems Branch at ARC to investigate the use of charged-coupled-device and IR cameras to meet the scientific requirements. Also examined is the effect of low levels of near-IR illumination on the circadian rhythm in rats.

Mian, Arshad↗

NASA NTRS1993

Activity-induced regulation of myosin isoform distribution - Comparison of two contractile activity programs

This study examined the role of specific types of contractile activity in regulating myosin heavy chain (MHC) isoform expression in rodent soleus. A combination of hindlimb suspension (SN) and two programmed contractile training activity paradigms, either isometric contractile activity (ST-IM) or high-load slowly shortening isovelocity activity, were utilized. Both training paradigms increased muscle mass compared with SN alone. However, only ST-IM resulted in a partial prevention of the suspension-induced decrease in type I MHC. With the use of a fluorescently labeled antibody to type IIa MHC, the distribution of MHCs among fibers was examined immunohistochemically. In SN, the percentage of cells staining positive for type IIa MHC was increased but the staining intensity of the positively staining cells was unchanged compared with control cells. In the ST-IM soleus, the percentage of positively staining fibers was unchanged but the intensity of the positively staining cells was decreased compared with SN values. These results suggest that 1) isometric contractile activity is more effective than isovelocity activity in preventing suspension-induced shifts in soleus MHC distribution and 2) changes associated with both suspension and training occur in only a small number of fibers, with the majority of fibers apparently unresponsive to these interventions.

Diffee, Gary M.↗

NASA NTRS1991

Mechanistic studies on reduced exercise performance and cardiac deconditioning with simulated zero gravity

The primary purpose of this research is to study the physiological mechanisms associated with the exercise performance of rats subjected to conditions of simulated weightlessness. A secondary purpose is to study related physiological changes associated with other systems. To facilitate these goals, a rodent suspension model was developed (Overton-Tipton) and a VO2 max testing procedure was perfected. Three methodological developments occurred during this past year deserving of mention. The first was the refinement of the tail suspension model so that (1) the heat dissipation functions of the caudal artery can be better utilized, and (2) the blood flow distribution to the tail would have less external constriction. The second was the development on a one-leg weight bearing model for use in simulated weightlessness studies concerned with change in muscle mass, muscle enzyme activity, and hind limb blood flow. The chemical body composition of 30 rats was determined and used to develop a prediction equation for percent fat using underwater weighing procedures to measure carcass specific gravity and to calculate body density, body fat, and fat free mass.

Tipton, Charles M.↗

NASA NTRS1993

STS-54 Space Shuttle mission report

The STS-54 Space Shuttle Program Mission Report is a summary of the Orbiter, External Tank (ET), Solid Rocket Booster/Redesigned Solid Rocket Motor (SRB/RSRM), and the Space Shuttle Main Engine (SSME) subsystems performance during this fifty-third flight of the Space Shuttle Program, and the third flight of the Orbiter vehicle Endeavour (OV-105). In addition to the Orbiter, the flight vehicle consisted of an ET, which was designated ET-51; three SSME's, which were serial numbers 2019, 2033, and 2018 in positions 1, 2, and 3, respectively; and two retrievable and reusable SRB's which were designated BI-056. The lightweight RSRM's that were installed in each SRB were designated 360L029A for the left SRB, and 360L029B for the right SRB. The primary objectives of this flight were to perform the operations to deploy the Tracking and Data Relay Satellite-F/Inertial Upper Stage payload and to fulfill the requirements of the Diffuse X-Ray Spectrometer (DXS) payload. The secondary objective was to fly the Chromosome and Plant Cell Division in Space (CHROMEX), Commercial Generic Bioprocessing Apparatus (CGBA), Physiological and Anatomical Rodent Experiment (PARE), and the Solid Surface Combustion Experiment (SSCE). In addition to presenting a summary of subsystem performance, this report also discusses each Orbiter, ET, SSME, SRB, and RSRM in-flight anomaly in the applicable section of the report. The official tracking number for each in-flight anomaly, assigned by the cognizant project, is also shown. All times are given in Greenwich mean time (G.m.t.) and mission elapsed time (MET).

Fricke, Robert W., Jr.↗

NASA NTRS1990

Investigating motion sickness using the conditioned taste aversion paradigm

The avoidance of foods which are associated with uncomfortable or aversive internal states has long been recognized. Many people are aware, either directly or via anecdotal reports, of individuals who avoid foods which were eaten just before the onset of sickness. Awareness of this phenomenon can be traced to the writings of John Locke. The disruption of diet during cancer therapy is sometimes ascribed to the attribution of an unpleasant quality to foods eaten preceding the sickness induced by therapy itself. In addition, it has long been recognized by the manufacturers of rodent poisons that animals avoid the injection of food treated with nonlethal doses of poison. An important part of the laboratory study of this phenomenon was directed toward studying the role learning plays in this type of avoidance behavior. Following the lead of Garcia and his associates, this avoidance has come to be interpreted as arising from a form of classical conditioning. In typical laboratory studies of this behavior, a novel food is ingested just prior to exposure to some stimulus, commonly poisoning or irradiation, which produces illness. Following the terminology of classical conditioning, it is common to describe this procedure as one of 'pairing' a conditioned stimulus (CS), the novel food, with an unconditioned stimulus (US), the illness induced by toxicosis or irradiation. Avoidance of the food in succeeding feeding opportunities is viewed as a learned response or a conditioned taste aversion (CTA). Garcia et al. asserted that motion sickness could produce 'gustatory' aversions, but passive motion was first reported as an US to establish CTA by Green and Rachlin. The purpose is to review the manner in which CTA has been used to study motion sickness. Numerous reviews concentrating on other aspects of CTA are available in the existing literature. Readers are encouraged to consult the various papers and edited books for extensive information on other aspects of this literature.

Robert A Fox↗

NASA NTRS1994

Cytokines and immune surveillance in humans

Evidence from both human and rodent studies has indicated that alterations in immunological parameters occur after space flight. Among the parameters shown, by us and others, to be affected is the production of interferons. Interferons are a family of cytokines that are antiviral and play a major role in regulating immune responses that control resistance to infection. Alterations in interferon and other cytokine production and activity could result in changes in immunity and a possible compromise of host defenses against both opportunistic and external infections. The purpose of the present study is to explore further the effects of space flight on cyotokines and cytokine-directed immunological function. Among the tests carried out are interferon-alpha production, interferon-gamma production, interleukin-1 and -2 production, signal transduction in neutrophils, signal transduction in monocytes, and monocyte phagocytic activity. The experiments will be performed using peripheral blood obtained from human subjects. It is our intent to eventually carry out these experiments using astronauts as subjects to determine the effects of space flight on cytokine production and activity. However, these subjects are not currently available. Until they become available, we will carry out these experiments using subjects maintained in the bed-rest model for microgravity.

Sonnenfeld, Gerald↗

NASA NTRS1993

Skeletal muscle metabolism in hypokinetic rats

This grant focused on the mechanisms of metabolic changes associated with unweighting atrophy and reduced growth of hind limb muscles of juvenile rats. Metabolic studies included a number of different areas. Amino acid metabolic studies placed particular emphasis on glutamine and branched-chain amino acid metabolism. These studies were an outgrowth of understanding stress effects and the role of glucocorticoids in these animals. Investigations on protein metabolism were largely concerned with selective loss of myofibrillar proteins and the role of muscle proteolysis. These investigations lead to finding important differences from denervation and atrophy and to define the roles of cytosolic versus lysosomal proteolysis in these atrophy models. A major outgrowth of these studies was demonstrating an ability to prevent atrophy of the unweighted muscle for at least 24 hours. A large amount of work concentrated on carbohydrate metabolism and its regulation by insulin and catecholamines. Measurements focused on glucose transport, glycogen metabolism, and glucose oxidation. The grant was used to develop an important new in situ approach for studying protein metabolism, glucose transport, and hormonal effects which involves intramuscular injection of various agents for up to 24 hours. Another important consequence of this project was the development and flight of Physiological-Anatomical Rodent Experiment-1 (PARE-1), which was launched aboard Space Shuttle Discovery in September 1991. Detailed descriptions of these studies can be found in the 30 peer-reviewed publications, 15 non-reviewed publications, 4 reviews and 33 abstracts (total 82 publications) which were or are scheduled to be published as a result of this project. A listing of these publications grouped by area (i.e. amino acid metabolism, protein metabolism, carbohydrate metabolism, and space flight studies) are included.

Tischler, Marc E.↗

NASA NTRS1994

Fluence-based relative biological effectiveness for charged particle carcinogenesis in mouse Harderian gland

Neoplasia in the rodent Harderian gland has been used to determine the carcinogenic potential of irradiation by HZE particles. Ions from protons to lanthanum at energies up to 670 MeV/a have been used to irradiate mice, and prevalence of Harderian gland tumors has been measured 16 months after irradiation. The Relative Biological Effectiveness (RBE) for tumor induction has been expressed as the RBE(sub max), which is the ratio of the initial slopes of the dose vs prevalence curve. The RBE(sub max) has been found to be approximately 30 for ions with Linear Energy Transfer (LET) values in excess of 100 keV/micrometer. Analysis on the basis of fluence as a substitute for dose has shown that on a per particle basis all of the ions with LET values in excess of 100 keV/micrometer have equal effectiveness. An analysis of the probabilities of ion traversals of the nucleus has shown that for these high stopping powers that a single hit is effective in producing neoplastic transformation.

Alpen, E. L.↗

NASA NTRS1994

A metabolic cage for the hindlimb suspended rat

Hindlimb suspension has been successfully used to simulate the effects of microgravity in rats. The cage and suspension system developed by E. R. Holton is designed to produce a headward shift of fluid and unload the hindlimbs in rodents, causing changes in bone and muscle similar to those in animals and humans exposed to spaceflight. While the Holton suspension system simulates many of the conditions observed in the spaceflight animal, it does not provide for the collection of urine and feces needed to monitor some metabolic activities. As a result, only limited information has been gathered on the nutritional status, and the gastrointestinal and renal function of animals using that model. Although commercial metabolic cages are available, they are usually cylindrical and require a centrally located suspension system and thus, do not readily permit movement of the rats. The limited floor space of commercial cages may affect comparisons with studies using the Holton model which has more than twice the living space of most commercially available cages. To take advantage of the extra living space and extensive data base that has been developed with the Holton model, Holton's cage was modified to make urine and fecal collections possible.

Evans, J.↗

NASA NTRS1993

The Biological Flight Research Facility

NASA Ames Research Center (ARC) is building a research facility, the Biological Flight Research Facility (BFRF), to meet the needs of life scientists to study the long-term effects of variable gravity on living systems. The facility will be housed on Space Station Freedom and is anticipated to operate for the lifetime of the station, approximately thirty years. It will allow plant and animal biologists to study the role of gravity, or its absence, at varying gravity intensities for varying periods of time and with various organisms. The principal difference between current Spacelab missions and those on Space Station Freedom, other than length of mission, will be the capability to perform on-orbit science procedures and the capability to simulate earth gravity. Initially the facility will house plants and rodents in habitats which can be maintained at microgravity or can be placed on a 2.5 meter diameter centrifuge. However, the facility is also being designed to accommodate future habitats for small primates, avian, and aquatic specimens. The centrifuge will provide 1 g for controls and will also be able to provide gravity from 0.01 to 2.0 g for threshold gravity studies as well as hypergravity studies. Included in the facility are a service unit for providing clean chambers for the specimens and a glovebox for manipulating the plant and animal specimens and for performing experimental protocols. The BFRF will provide the means to conduct basic experiments to gain an understanding of the effects of microgravity on the structure and function of plants and animals, as well as investigate the role of gravity as a potential countermeasure for the physiological changes observed in microgravity.

Johnson, Catherine C.↗

NASA NTRS1994

Spaceflight and immune responses of Rhesus monkeys

Evidence from both human and rodent studies indicates that alterations in immunological parameters occur after space flight. The objective of this project is to determine the effects of space flight on immune responses of Rhesus monkeys. The expected significance of the work is a determination of the range of immunological functions of the Rhesus monkey, a primate similar in many ways to man, affected by space flight. Changes in immune responses that could yield alterations in resistance to infection may be determined as well as the duration of alterations in immune responses. Additional information on the nature of cellular interactions for the generation of immune responses may also be obtained.

Sonnenfeld, Gerald↗

NASA NTRS1995

Experiment kits for processing biological samples inflight on SLS-2

This paper describes development of an innovative, modular approach to packaging the instruments used to obtain and preserve the inflight rodent tissue and blood samples associated with hematology experiments on the Spacelab Life Sciences-2 (SLS-2) mission. The design approach organized the multitude of instruments into twelve 5- x 6- x l-in. kits which were each used for a particular experiment. Each kit contained the syringes, vials, microscope slides, etc., necessary for processing and storing blood and tissue samples for one rat on a particular day. A total of 1245 components, packaged into 128 kits and stowed in 17 Zero(registered trademark) boxes, were required. Crewmembers found the design easy to use and laid out in a logical, simple configuration which minimized chances for error during the complex procedures in flight. This paper also summarizes inflight performance of the kits on SLS-2.

Savage, P. D.↗

NASA NTRS1994

The Rhesus monkey as a model for testing the immunological effects of space flight

The Rhesus monkey has been proposed as a model for the effects of space flight on immunity. In order to determine the feasibility of the use of the Rhesus monkey as a model, we studied the use of Rhesus monkey cells for immunological procedures that have been shown to be affected by space flight in both rodents and humans. We have shown that both lymph node cells and peripheral blood leukocytes can be stained with monoclonal antibodies to detect the following surface markers: CD4, CD-8, Ia and surface immunoglobulin. Also, the level of Ia antigen expression was increased by treatment of the cells with human interferon-gamma. In addition, cells were induced to produce interferons and interleukins. Isolated neutrophils also demonstrated increased oxidative burst. These data indicate that the Rhesus monkey will be a useful model for space flight studies of immunity.

Sonnenfeld, G.↗

NASA NTRS1991

[STS-48 Mission Highlights Resource Tape. Part 1 of 2]

In this first part of a two part video mission-highlights set, the flight of the STS-48 Space Shuttle Orbiter Discovery is reviewed. The flight crew consisted of: J. O. Creighton (Commander); Ken Reightler (Pilot); Charles 'Sam' Gemar (Mission Specialist); James 'Jim' Buchli (MS); and Mark Brown (MS). Step-by-step pre-launch and sunset launch sequences are shown with accompanying shots inside the Mission Control Center. The primary goal of this mission was the deployment of Upper Atmosphere Research Satellite (UARS). Other (secondary) payloads included: the MidDeck Zero Gravity Experiment (MODE); the Sam/Cream device; the Shuttle Activation Monitor/Cosmic Ray Effects and Activation Monitor Experiment; and the Physiology and Anatomical Rodent Experiment (PARE). Crew activities were shown, along with Earth views (Aurora Borealis (B/W), light from the Kuwait oil fires, lightning over Italy and other areas, polar regions and ice caps, and the United States at night (B/W)). This was the thirteenth flight of the Space Shuttle Discovery. A night landing is shown.

Source record↗

NASA NTRS1995

Spacelab Life Sciences-1

This report provides an historical overview of the Spacelab Life Sciences-1 (SLS-1) mission along with the resultant biomaintenance data and investigators' findings. Only the nonhuman elements, developed by Ames Research Center (ARC) researchers, are addressed herein. The STS-40 flight of SLS-1, in June 1991, was the first spacelab flown after 'return to orbit', it was also the first spacelab mission specifically designated as a Life Sciences Spacelab. The experiments performed provided baseline data for both hardware and rodents used in succeeding missions.

Dalton, Bonnie P.↗