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At least 325 records · Page 18

The rotating spectrometer: Biotechnology for cell separations

An instrument for biochemical studies, called the rotating spectrometer, separates previously inseparable cell cultures. The rotating spectrometer is intended for use in pharmacological studies which require fractional splitting of heterogeneous cell cultures based on cell morphology and swimming behavior. As a method to separate and concentrate cells in free solution, the rotating method requires active organism participation and can effectively split the large class of organisms known to form spontaneous patterns. Examples include the biochemical star, an organism called Tetrahymena pyriformis. Following focusing in a rotating frame, the separation is accomplished using different radial dependencies of concentrated algal and protozoan species. The focusing itself appears as concentric rings and arises from the coupling between swimming direction and Coriolis forces. A dense cut is taken at varying radii, and extraction is replenished at an inlet. Unlike standard separation and concentrating techniques such as filtration or centrifugation, the instrument is able to separate motile from immotile fractions. For a single pass, typical split efficiencies can reach 200 to 300 percent compared to the inlet concentration.

Noever, David A.↗

Symbol Tables and Branch Tables: Linking Applications Together

This document explores the computer techniques used to execute software whose parts are compiled and linked separately. The computer techniques include using a branch table or indirect address table to connect the parts. Methods of storing the information in data structures are discussed as well as differences between C and C++.

Handler, Louis M.↗

Summary of in-flight flow visualization obtained from the NASA high alpha research vehicle

A summary of the surface and off-surface flow visualization results obtained in flight on the F-18 high alpha research vehicle (HARV) is presented, highlighting the extensive 3-D vortical flow on the aircraft at angles of attack up to 50 degs. The emitted fluid technique, as well as tufts and flow cones, were used to document the surface flow. A smoke generator system injected smoke into the vortex cores generated by the forebody and leading edge extensions (LEXs). Documentation was provided by onboard still and video, by air-to-air, and by postflight photography. The surface flow visualization techniques revealed laminar separation bubbles near the forebody apex, lines of separation on the forebody and LEX, and regions of attached and separated flow on the wings and fins. The off-surface flow visualization techniques showed the path of the vortex cores on the forebody and LEX as well as the LEX vortex core breakdown location. An interaction between the forebody and LEX vortices was noted. The flow over the surfaces of the vertical tail was categorized into regions of attached, unsteady, or separated flow using flow tufts.

Fisher, David F.↗

Control techniques to improve Space Shuttle solid rocket booster separation

The present Space Shuttle's control system does not prevent the Orbiter's main engines from being in gimbal positions that are adverse to solid rocket booster separation. By eliminating the attitude error and attitude rate feedback just prior to solid rocket booster separation, the detrimental effects of the Orbiter's main engines can be reduced. In addition, if angular acceleration feedback is applied, the gimbal torques produced by the Orbiter's engines can reduce the detrimental effects of the aerodynamic torques. This paper develops these control techniques and compares the separation capability of the developed control systems. Currently with the worst case initial conditions and each Shuttle system dispersion aligned in the worst direction (which is more conservative than will be experienced in flight), the solid rocket booster has an interference with the Shuttle's external tank of 30 in. Elimination of the attitude error and attitude rate feedback reduces that interference to 19 in. Substitution of angular acceleration feedback reduces the interference to 6 in. The two latter interferences can be eliminated by atess conservative analysis techniques, that is, by using a root sum square of the system dispersions.

Tomlin, D. D.↗

Studies of flow separation and stalling on one- and two-element airfoils at low speeds

Research has been conducted on the nature of airfoil behavior at pre- and post-separated angles of attack. Detailed wind tunnel studies have been made of boundary layer and wake fields for the GA(W)-1 airfoil, and the airfoil with a 0.3 chord Fowler flap. Experimental data are compared with theoretical predictions from a multi-element viscous flow computer program. Theoretical predictions are reasonably accurate for unseparated flows, but have serious errors when separation is present. Some recent techniques for modeling post-separated flow behavior are discussed in light of the present experiments.

Seetharam, H. C.↗

An expert system shell for inferring vegetation characteristics: Interface for the addition of techniques (Task H)

All the NASA VEGetation Workbench (VEG) goals except the Learning System provide the scientist with several different techniques. When VEG is run, rules assist the scientist in selecting the best of the available techniques to apply to the sample of cover type data being studied. The techniques are stored in the VEG knowledge base. The design and implementation of an interface that allows the scientist to add new techniques to VEG without assistance from the developer were completed. A new interface that enables the scientist to add techniques to VEG without assistance from the developer was designed and implemented. This interface does not require the scientist to have a thorough knowledge of Knowledge Engineering Environment (KEE) by Intellicorp or a detailed knowledge of the structure of VEG. The interface prompts the scientist to enter the required information about the new technique. It prompts the scientist to enter the required Common Lisp functions for executing the technique and the left hand side of the rule that causes the technique to be selected. A template for each function and rule and detailed instructions about the arguments of the functions, the values they should return, and the format of the rule are displayed. Checks are made to ensure that the required data were entered, the functions compiled correctly, and the rule parsed correctly before the new technique is stored. The additional techniques are stored separately from the VEG knowledge base. When the VEG knowledge base is loaded, the additional techniques are not normally loaded. The interface allows the scientist the option of adding all the previously defined new techniques before running VEG. When the techniques are added, the required units to store the additional techniques are created automatically in the correct places in the VEG knowledge base. The methods file containing the functions required by the additional techniques is loaded. New rule units are created to store the new rules. The interface that allow the scientist to select which techniques to use is updated automatically to include the new techniques. Task H was completed. The interface that allows the scientist to add techniques to VEG was implemented and comprehensively tested. The Common Lisp code for the Add Techniques system is listed in Appendix A.

Harrison, P. Ann↗

Recovery and separation of precious metals from space

During the past year a viable procedure centered around centrifugal partition chromatography (CPC), a multistage liquid-liquid partitioning technique for the separation of precious metals (Pt, Pd, Rh, Ir, Os, Ru), was developed. Stable and inexpensive ligands that can be readily recycled to achieve the separations of the precious metals were identified. The separation methods developed so far yield three separate fractions: Pt, Pd, and Rh-Ir. The Rh-Ir pair can be separated in a subsequent run. The total amount of precious metals separated in a single experiment varied from 1 to 50 mg. The factors affecting the efficiencies of these separations were studied. The kinetics of the decomposition of the complex and the ion pair have a major bearing on these efficiencies, with slow kinetics resulting in poor efficiencies. The methods for the improvement of the efficiencies were also investigated. For example, significant improvement in the efficiencies and separation times for Pt and Pd were achieved by the use of chloride gradient in the mobile phase. Two papers were published and talks were presented on our work at the FACSS meeting in Anaheim, Oct. 1991, and at the Pittsburgh Conference in New Orleans, Mar. 1992.

Freiser, H.↗

Cloud type separation by spectral differencing of image pairs

A technique is presented for discriminating different cloud types through an image subtraction of visible and infrared SMS/GOES picture pairs. The technique emphasizes how one could separate snow from clouds and identify cirrus by the subtraction method. Quantitative threshold values are shown which can be used in an objective manner to make this separation. Use is made of an all-digital image display device allowing such mathematical operations to be performed on satellite data. Techniques such as this can be made operational through the interfacing of the image analysis system with a direct-readout SMS/GOES ground station and distribution network.

Reynolds, D. W.↗

Evidence for amino-acids of extraterrestrial origin in the Orgueil meteorite.

The problems of separation of contaminant from indigenous amino-acids in meteorites can be largely overcome by applying gas chromatographic techniques for the separation of D,L enantiomers of amino-acids as their diastereomeric derivatives and the identification of these compounds by gas chromatographic retention times and mass spectral fragmentation patterns. Two samples of Orgueil meteorite were examined. The results show that there are amino-acids indigenous to the Orgueil meteorite in addition to those present as contaminants.

Lawless, J. G.↗

Antibody enhancement of free-flow electrophoresis

Specific T cell clones and antibodies (ABs) were developed to study the efficiency of purifying closely associated T cells using Continuous Flow Electrophoresis System. Enhanced separation is accomplished by tagging cells first with ABs directed against the antigenic determinants on the cell surface and then with ABs against the Fc portion of the first AB. This second AB protrudes sufficiently beyond the cell membrane and glycocalyx to become the major overall cell surface potential determinant and thus causes a reduction of electrophoretic mobility. This project was divided into three phases. Phase one included development of specific T cell clones and separation of these specific clones. Phase two extends these principles to the separation of T cells from spleen cells and immunized lymph node cells. Phase three applies this double antibody technique to the separation of T cytotoxic cells from bone marrow.

Cohly, H. H. P.↗

Novel Electrochemical Technique: Serial Open Circuit Potentiometry to Examine the Separability of Lanthanides in Molten Salts

The accumulation of lanthanide fission products in molten salts reduces the efficiency of pyroprocessing used nuclear fuel. Lanthanide fission products are notoriously difficult to separate due to their similar physical and electrical properties. We developed a novel electrochemical technique called serial open circuit potentiometry (SOCP) which allows us to delineate between metal deposition reactions with similar reduction potentials to great precision. SOCP has several advantages over traditional open circuit potentiometry including its ability to create 3D plots that describe all metal deposition reactions in a system. This information can be used to precisely determine equilibrium potentials of metal deposition reactions and quantify their error, determine nucleation potentials, and determine the nucleation and growth mechanism. In this work, we demonstrated the effectiveness of SOCP by studying molten salt mixtures containing CeCl3, GdCl3, and both CeCl3 and GdCl3 in LiCl-KCl eutectic.

11 - NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Separated flow over bodies of revolution using an unsteady discrete-vorticity cross wake. Part 1: Theory and application

A method is developed to determine the flow field of a body of revolution in separated flow. The technique employed is the use of the computer to integrate various solutions and solution properties of the sub-flow fields which made up the entire flow field without resorting to a finite difference solution to the complete Navier-Stokes equations. The technique entails the use of the unsteady cross flow analogy and a new solution to the required two-dimensional unsteady separated flow problem based upon an unsteady, discrete-vorticity wake. Data for the forces and moments on aerodynamic bodies at low speeds and high angle of attack (outside the range of linear inviscid theories) such that the flow is substantially separated are produced which compare well with experimental data. In addition, three dimensional steady separation regions and wake vortex patterns are determined.

Marshall, F. J.↗

Separated flow over a body of revolution

A method is developed to determine the flowfield of a body of revolution in separated flow. The technique employed is the use of the computer to integrate various solutions and solution properties of the sub-flowfields which make up the entire flowfield without resorting to a finite difference solution to the complete Navier-Stokes equations. The technique entails the use of the unsteady cross flow analogy and a new solution to the required two-dimensional unsteady separated flow problem based upon an unsteady discrete-vorticity wake. Data for the forces and moments on bodies of revolution at high angle of attack (outside the range of linear inviscid theories) such that the flow is substantially separated are produced which compare well with experimental data at low speeds. In addition, three-dimensional steady separation regions and wake vortex patterns are determined.

Marshall, F. J.↗

Discrimination of hydrothermal alteration mineral assemblages at Virginia City, Nevada, using the airborne imaging spectrometer

The purpose of this study is to use airborne imaging spectrometer data to discriminate hydrothermal alteration mineral assemblages associated with silver and gold mineralization at Virginia City, NV. The data is corrected for vertical striping and sample gradients, and converted to flat-field logarithmic residuals. Log residual spectra from areas known to be altered are compared to field spectra for kaolinitic, illitic, sericitic, and propylitic alteration types. The areal distributions of these alteration types are estimated using a spectral matching technique. Both visual examination of spectra and the matching techniques are effective in distinguishing kaolinitic, illitic, and propylitic alteration types from each other. However, illitic and sericitic alteration cannot be separated using these techniques because the spectra of illite and sericite are very similar. A principal components analysis of 14 channels in the 2.14-2.38 micron wavelength region is also successful in discriminating and mapping illitic, kaolinitic, and propylitic alteration types.

Hutsinpiller, Amy↗

Improved extraction technique for biological fluids

Liquid-liquid extraction technique speeds up separation of biological fluids into number of compounds. This eliminates agitation, emulsion formation, centrifugation, mechanical separation of phases, filtration, and other steps that have been used previously. Extraction efficiencies are equal or better than current manual liquid-liquid extraction techniques.

Jahnsen, V. J.↗

Polarization-separated double-imaging spectroscopy for weakly-ionized plasma diagnostics

Laser Thomson scattering, which provides thermal properties of electrons, has been extensively utilized in plasma diagnostics. However, being a non-resonant linear light scattering technique, it often encounters several challenges in weakly-ionized plasma diagnostics due to spectral overlap with different scatterings induced from other existing species, including Mie, Rayleigh, and rotational Raman scatterings. To address this challenge, we propose an imaging spectroscopy technique, polarization-separated double-imaging spectroscopy (PoDIS), which selectively separates specific scatterings based on their polarization characteristics. Using an atmospheric plasma jet as a plasma source for demonstration, we show that PoDIS can effectively separate rotational Raman and Thomson scatterings from a superimposed spectrum without prior knowledge or assumptions about the thermal properties of neutrals or electrons. This separation enables independent fitting of the rotational Raman and Thomson scattering spectra, allowing for precise determination of the thermal properties of neutral particles and electrons separately.

Bak, Junhwi [Texas A&M University, College Station↗

Design and Performance of a Gas Chromatograph for Automatic Monitoring of Pollutants in Ambient Air

In recent years, interest in air pollution constituents has focused on carbon monoxide and hydrocarbons as prime components of polluted air. Instrumental methods have been developed, and commercial instruments for continuous monitoring of these components have been available for a number of years. For the measurement of carbon monoxide, non-dispersive infrared spectroscopy has been the accepted tool, in spite of its marginal sensitivity at low parts-per-million levels. For continuously monitoring total hydrocarbons, the hydrogen flame ionization analyzer has been widely accepted as the preferred method. The inadequacy of this latter method became evident when it was concluded that methane is non-reactive and cannot be considered a contaminant even though present at over 1 ppm in the earth's atmosphere. Hence, the need for measuring methane separately became apparent as a means of measuring the reactive and potentially harmful non-methane hydrocarbons fraction. A gas chromatographic method for the measurement of methane and total hydrocarbons which met these requirements has been developed. In this technique, methane was separated on conventional gas chromatographic columns and detected by a hydrogen flame ionization detector (FID) while the total hydrocarbons were obtained by introducing a second sample directly into the FID without separating the various components. The reactive, or non-methane hydrocarbons, were determined by difference. Carbon monoxide was also measured after converting to methane over a heated catalyst to render it detectable by the FID. The development of this method made it possible to perform these measurements with a sensitivity of as much as 1 ppm full scale and a minimum detectability of 20 ppb. Incorporating this technique, criteria were developed by APCO for a second generation continuous automatic instrument for atmospheric monitoring stations.

Villalobos, R.↗