Deep tissue profiling of Populus stem at single nucleus level reveals uncharacterized cell types and cell-specific gene regulatory networks
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Biotechnology & Applied Microbiology↗
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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
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Understanding, predicting and mitigating cell-to-cell cascading failure in lithium-ion battery modules
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Hewson, John Christopher↗
MassIVE MSV000100641 - Multilayered regulation by RNA thermometers enables precise control of Cas9 expression in E. coli: Cas9-based genome editing technologies can rapidly generate mutations to probe a diverse array of mutant genotypes. However, aberrant Cas9 nuclease translation and activity can occur despite the use of inducible promoters to control expression, leading to extensive cell death. This background killing caused by promoter leakiness severely limits the application of Cas9 for generating mutant libraries because of the potential for population skew. We demonstrate the utility of temperature sensitive RNA elements as a layer of post-transcriptional regulation to reduce the impact of promoter leak. We observe significant temperature-dependent increases in cell survival when certain RNA thermometers (RNATs) are placed upstream of the cas9 coding sequence. We also show that the most highly repressing RNAT, hsp17rep, significantly reduces population skew with a library of characterized guide RNAs in Escherichia coli. This strategy should be applicable to all bacterial Cas9-based methods and technologies.
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Alexander, William G. [Oak Ridge National Laborato↗
Evaluation program for secondary spacecraft cells. acceptance test of general electric company 5.0 ampere-hour nimbus cells
Functional acceptance tests of five ampere-hour Nimbus nickel-cadmium secondary spacecraft batteries
SPACECRAFT↗
Evaluation program for secondary spacecraft cells. acceptance test of gulton industries, inc. 6.0 ampere-hour adhydrode cells
Functional acceptance tests of six ampere-hour adhydrode nickel cadmium secondary spacecraft batteries
NICKEL-CADMIUM BATTERY↗
Significance of the process of postradiation regeneration of genetic structures for the radiosensitivity of cells. Communication 1 - Quantitative laws of post radiation restoration of yeast cells
Postradiation regeneration of genetic structures and degree to which cytoplasm damage affects chromosome restoration process following exposure of Saccharomyces to Cobalt 60
BIOREGENERATION↗
Significance of the process of postradiation regeneration of genetic structures for the radiosensitivity of cells. Communication 2 - Radiosensitivity of yeast cells of various ploidy
Radiosensitivity of Saccharomyces of various ploidy and postradiation regeneration of genetic structures following exposure to cobalt 60
BIOREGENERATION↗
Evaluation program for secondary spacecraft cells. Acceptance test of Gulton Industries, Inc. 1.25 ampere-hour nickel cadmium cells with high overcharge capabilities
Performance of sealed nickel-cadmium spacecraft batteries with high overcharge capability
SPACECRAFT↗
Evaluation program for secondary spacecraft cells - General performance test of General Electric 6.0 ampere-hour nickel cadmium secondary spacecraft cells
Environmental and performance tests of 6.0 ampere- hour sealed nickel cadmium secondary spacecraft batteries
Source record↗
Evaluation program for secondary spacecraft cells. Evaluation of storage methods, open circuit versus continuous trickle charge, Sonotone 3.5 ampere-hour sealed nickel- cadmium secondary spacecraft cells
Storage methods for secondary nickel cadmium spacecraft batteries
Christy, D. E.↗
Evaluation program for secondary spacecraft cells. Evaluation of storage methods, open circuit versus continuous trickle charge, sonotone 3.5 ampere-hour sealed nickel- cadmium secondary spacecraft cells
Performance tests of nickel cadmium batteries for evaluation of storage methods
Thomas, R. E.↗
Evaluation program for secondary spacecraft cells; acceptance tests of General Electric 4.5 ampere-hour nickel-cadmium cells, Nimbus type
Results are presented of acceptance tests conducted on eleven nickel cadmium batteries of the Nimbus satellite type.
Christy, D. E.↗
Irradiation of solar cell candidates for the ATS-F solar cell flight experiment.
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Goldhammer, L. J.↗
Evaluation program for secondary spacecraft cells: initial evaluation tests of 20.0 ampere-hour nickel-cadmium spacecraft cells manufactured by Gulton Industries, Incorporated
The report describes testing and performance of 20.0 ampere-hour Ni-Col spacecraft batteries.
Harkness, J. D.↗
Solar cell performance in terrestrial sunlight and Variation of solar cell efficiency with air mass and Microclimatological influences on two solar modules
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Castle, J. A.↗
Sensitivity of solar cell performance to atmospheric variables. 1: Single cell
For abstract, see N77-26623.
Klucher, T. M.↗
Sensitivity of solar-cell performance to atmospheric variables. 2: Dissimilar cells at several locations
For abstract, see N77-26624.
Klucher, T. M.↗
Do the design concepts used for the space flight hardware directly affect cell structure and/or cell function ground based simulations
The use of clinostats and centrifuges to explore the hypogravity range between zero and 1 g is described. Different types of clinostat configurations and clinostat-centrifuge combinations are compared. Some examples selected from the literature and current research in gravitational physiology are presented to show plant responses in the simulated hypogravity region of the g-parameter (0 is greater than g is greater than 1). The validation of clinostat simulation is discussed. Examples in which flight data can be compared to clinostat data are presented. The data from 3 different laboratories using 3 different plant species indicate that clinostat simulation in some cases were qualitatively similar to flight data, but that in all cases were quantitatively different. The need to conduct additional tests in weightlessness is emphasized.
Chapman, David K.↗