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All sourcesNASA NTRSDOE OSTI
DOE OSTI2023

Physical Education Teachers’ Representations of Their Training to Promote the Inclusion of Students with Disabilities

School inclusion is based on the need to adopt and implement a holistic view of education, training, and human development embodied in the idea of everyone, for everyone. In the context of Physical Education (PE), there are still several constraints to the realization of this universal desideratum. Among these, teacher training and qualification for the inclusion of students with Specific Health Needs (SHNs) stands out. That is, students with physical and mental health problems whose impact is significantly manifested in the learning process. Thus, the objective of this study was to identify the representations of PE teachers about their training to develop inclusive processes with students with SES. Participants in this study were 151 PE teachers from different regions and districts of Portugal (Algarve, Aveiro, Castelo Branco, Lisbon, Porto, and Viseu) who had 23.6 ± 8.1 years of teaching service. Teachers answered an online questionnaire, on the Google Forms platform, with open and closed questions about their education and training to develop inclusive processes in PE. The results indicate two significant dimensions: (1) initial training for teaching inclusive PE and (2) continuous training for inclusion. Regarding initial training, a large majority of the teachers under study, at the end of their initial training, did not have the essential skills to teach PE to students with SES. It was also identified that a large majority reported not having had any contact with students with SES throughout their training process for teaching. It was also recognized that this training was not adjusted to the development of intervention skills with students with SHN. Regarding continuous training, it was identified that attendance at this training increased their skills to teach PE to students with SHN. Workshops/actions/training courses are the main training models adopted. However, it is recognized that the training provided does not respond concretely to their training needs to intervene with students with SHN, since teachers essentially seek to improve intervention in the context of inclusive physical education. We conclude that teacher training for inclusion is not yet fully adjusted to the reality of the inclusive school paradigm. In this sense, in practical terms, the following are suggested: (1) the need for reinforcement in study plans with specific and long-term curricular units; (2) the introduction of real practice components in context; and (3) supervised pedagogical practice in diverse contexts.

Celestino, Tadeu (ORCID:0000000163087981)↗
DOE OSTI2022

Defining and Measuring Forest Dependence in the United States: Operationalization and Sensitivity Analysis

This manuscript helps bridge a gap between theoretical work that advocates for a broad view of forest dependence, and empirical work that has focused narrowly on economic measures. Background: Forest dependence has been widely recognized as a valuable concept for understanding human communities’ well-being and vulnerability to shocks and changes. Past theoretical literature has highlighted the importance of recognizing various types of dependence—environmental, economic, and social—yet past empirical literature on the topic in the United States has almost exclusively relied on measures of economic dependence such as employment and earnings from the traditional forest products sector. Objective and Methods: As a first step to bridge the gap between the theoretical and empirical, we reviewed the existing, publicly available, reliable, wall-to-wall data sources to identify alternate proxy measures for forest dependence. Data availability made the analysis feasible only at the county level—the administrative subdivisions of the state—or higher. Results and Conclusions: We created environmental, economic, and social criteria based on threshold levels of the following proxy variables: forest area, earnings, employment, and indigenous population. Using these criteria, we identified 524 counties to be potentially forest-dependent of 3140 total counties in the United States. The largest concentration was in the Pacific Northwest and Southeast regions, and a higher proportion were non-metro counties than metro. Varying the threshold levels significantly changes the number of counties identified but does not alter the overall geographic trends.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2019

Tethys Knowledge Management System: Working to Advance the Marine Renewable Energy Industry

Purpose: This paper discusses an ongoing process to assist with the advancement of worldwide understanding of the environmental effects of marine renewable energy (MRE) through a public, online knowledge management system known as Tethys (https://tethys.pnnl.gov). Understanding potential environmental impacts can reduce expensive monitoring requirements and promote growth of this emerging industry. Approach: Tethys acts as the central repository for information in a landscape where literature and knowledge are scattered and difficult to find, providing access for an international audience of stakeholders and the public. An intuitive and flexible design has been developed over 8 years of operation, pairing content curation with synthesis and engagement to enhance the impact of knowledge management. Findings: Tethys has become internationally recognized and trusted as an honest broker of information. A community of participants that use Tethys resources and contributes content has grown to almost 50,000 people around the world, annually. Engineering and siting challenges continue to stall the deployment and establishment of the MRE industry, but progress has been made towards retiring environmental risks and facilitating progression in deploying and operating devices. Practical Implications: The need for knowledge management is particularly important when information is scattered, as is often the case with emerging industries. Tethys serves as an example of intentional design and intuitive organization for knowledge management. Value of Paper: This paper discusses focused knowledge management methods with international cooperation and public engagement to advance an emerging technical industry.

Whiting, Jonathan M.↗
DOE OSTI2024

Higher education institutions can do more to lead society toward resilience and sustainability

Higher education institutions (HEIs) are beginning to recognize their role as leading societal institutions in the movement toward resilience and sustainability by empowering their students as sustainability stakeholders and leaders and recognizing the value of faculty and staff as facilitators in this mobilization toward a more sustainable future. This chapter identifies seven goals derived from academic research that can be used to foster student leadership in sustainability and support faculty and staff in facilitatory roles. To date, however, most research has focused on sustainability in the classroom environment or the establishment of living labs. To illuminate how these seven goals can be achieved, the chapter adopts stakeholder theory and conducts an integrated case study assessment. Overall, the chapter helps to (i) develop a stronger understanding of sustainability and resilience, (ii) identify best practices for empowering student leaders and to better understand the facilitatory role played by faculty and staff in achieving the seven goals, and (ii) offer further insights through a reflective case study.

Sustainability and Resilience, Sustainability Syst↗
DOE OSTI2018

RNA-dependent RNA targeting by CRISPR-Cas9

Double-stranded DNA (dsDNA) binding and cleavage by Cas9 is a hallmark of type II CRISPR-Cas bacterial adaptive immunity. All known Cas9 enzymes are thought to recognize DNA exclusively as a natural substrate, providing protection against DNA phage and plasmids. Here, we show that Cas9 enzymes from both subtypes II-A and II-C can recognize and cleave single-stranded RNA (ssRNA) by an RNA-guided mechanism that is independent of a protospacer-adjacent motif (PAM) sequence in the target RNA. RNA-guided RNA cleavage is programmable and site-specific, and we find that this activity can be exploited to reduce infection by single-stranded RNA phage in vivo. We also demonstrate that Cas9 can direct PAM-independent repression of gene expression in bacteria. These results indicate that a subset of Cas9 enzymes have the ability to act on both DNA and RNA target sequences, and suggest the potential for use in programmable RNA targeting applications.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2019

Engineering a conserved RNA regulatory protein repurposes its biological function in vivo

PUF (PUmilio/FBF) RNA-binding proteins recognize distinct elements. In C. elegans, PUF-8 binds to an 8-nt motif and restricts proliferation in the germline. Conversely, FBF-2 recognizes a 9-nt element and promotes mitosis. To understand how motif divergence relates to biological function, we first determined a crystal structure of PUF-8. Comparison of this structure to that of FBF-2 revealed a major difference in a central repeat. We devised a modified yeast 3-hybrid screen to identify mutations that confer recognition of an 8-nt element to FBF-2. We identified several such mutants and validated structurally and biochemically their binding to 8-nt RNA elements. Using genome engineering, we generated a mutant animal with a substitution in FBF-2 that confers preferential binding to the PUF-8 element. The mutant largely rescued overproliferation in animals that spontaneously generate tumors in the absence of puf-8. This work highlights the critical role of motif length in the specification of biological function.

Bhat, Vandita D.↗
DOE OSTI2022

Structure and flexibility of the yeast NuA4 histone acetyltransferase complex

The NuA4 protein complex acetylates histones H4 and H2A to activate both transcription and DNA repair. We report the 3.1-Å resolution cryo-electron microscopy structure of the central hub of NuA4, which flexibly tethers the histone acetyltransferase (HAT) and Trimer Independent of NuA4 involved in Transcription Interactions with Nucleosomes (TINTIN) modules. The hub contains the large Tra1 subunit and a core that includes Swc4, Arp4, Act1, Eaf1, and the C-terminal region of Epl1. Eaf1 stands out as the primary scaffolding factor that interacts with the Tra1, Swc4, and Epl1 subunits and contributes the conserved HSA helix to the Arp module. Using nucleosome-binding assays, we find that the HAT module, which is anchored to the core through Epl1, recognizes H3K4me3 nucleosomes with hyperacetylated H3 tails, while the TINTIN module, anchored to the core via Eaf1, recognizes nucleosomes that have hyperacetylated H2A and H4 tails. Together with the known interaction of Tra1 with site-specific transcription factors, our data suggest a model in which Tra1 recruits NuA4 to specific genomic sites then allowing the flexible HAT and TINTIN modules to select nearby nucleosomes for acetylation.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

The autophagy receptor NBR1 directs the clearance of photodamaged chloroplasts

The ubiquitin-binding NBR1 autophagy receptor plays a prominent role in recognizing ubiquitylated protein aggregates for vacuolar degradation by macroautophagy. Here, we show that upon exposing Arabidopsis plants to intense light, NBR1 associates with photodamaged chloroplasts independently of ATG7, a core component of the canonical autophagy machinery. NBR1 coats both the surface and interior of chloroplasts, which is then followed by direct engulfment of the organelles into the central vacuole via a microautophagy-type process. The relocalization of NBR1 into chloroplasts does not require the chloroplast translocon complexes embedded in the envelope but is instead greatly enhanced by removing the self-oligomerization mPB1 domain of NBR1. The delivery of NBR1-decorated chloroplasts into vacuoles depends on the ubiquitin-binding UBA2 domain of NBR1 but is independent of the ubiquitin E3 ligases SP1 and PUB4, known to direct the ubiquitylation of chloroplast surface proteins. Compared to wild-type plants, nbr1 mutants have altered levels of a subset of chloroplast proteins and display abnormal chloroplast density and sizes upon high light exposure. We postulate that, as photodamaged chloroplasts lose envelope integrity, cytosolic ligases reach the chloroplast interior to ubiquitylate thylakoid and stroma proteins which are then recognized by NBR1 for autophagic clearance. This study uncovers a new function of NBR1 in the degradation of damaged chloroplasts by microautophagy.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1971

Automatic recognition of vector and parallel operations in a higher level language

A compiler for recognizing statements of a FORTRAN program which are suited for fast execution on a parallel or pipeline machine such as Illiac-4, Star or ASC is described. The technique employs interval analysis to provide flow information to the vector/parallel recognizer. Where profitable the compiler changes scalar variables to subscripted variables. The output of the compiler is an extension to FORTRAN which shows parallel and vector operations explicitly.

Schneck, P. B.↗
NASA NTRS1972

A reconnaissance space sensing investigation of the crustal structure for a strip from the eastern Sierra Nevada to the Colorado Plateau: April 1971

The author has identified the following significant results. An area of anomalous linear topographic grain and color expressions was recognized in Apollo 9 and ERTS-1 imagery along the Colorado River of northwestern Arizona and southern Nevada. Field reconnaissance and analysis of U-2 photography has shown the anomaly to be a zone of north to north-northwest trending dike swarms and associated granitic plutons. The dikes vary in composition from rhyolite to diabase, with an average composition nearer rhyolite. Shearing and displacement of host rocks along dikes suggest dike emplacement along active fault zones. Post-dike deformation has resulted in shearing and complex normal faulting along a similar north-south trend. The epizonal plutonism and volcanism of this north-south belt appears to represent a structurally controlled volcanogenic province which ends abruptly in the vicinity of Lake Mead at a probable eastern extension of the Las Vegas Shear Zone. The magnitude and chronology of extensional faulting and plutonism recognized in the north-south zone, support the hypothesis that the Las Vegas Shear Zone is a transform fault separating two areas of crustal spreading.

Bechtold, I. C.↗
NASA NTRS1972

Environmental study of ERTS-1 imagery Lake Champlain Basin and Vermont

The author has idenfified the following significant results. A first approximation land-type map using three categories of classification was generated for the Burlington area. The identification and mapping of a major turbidity front separating turbid waters of the southern arm of Lake Champlain from the clearer main water mass was reported on RBV 1 and 2 imagery and on subsequent MSS bands 4 and 5. Significant industrial pollution of Lake Champlain has degraded environmental quality in certain sections of the lake. Wetlands were detected and recognized using a combination of RBV bands 2 and 3. Using first-look RBV band 2 imagery, major ice marginal features were identified by using tonal patterns associated with vegetative cover. Major rivers were detected and recognized through the use of RBV band 3 imagery and MSS bands 6 and 7.

Lind, A. O.↗
NASA NTRS1972

Identification of phenological stages and vegetative types for land use classification

The author has identified the following significant results. Two procedures for examining ERTS-1 imagery were investigated in cooperation with the photographic services section. Positive, 10X enlargements were produced of spectral bands 4, 6, and 7, for a portion of photo 1033-21020. Adequate detail remained to recognize physical features such as streams, glaciers, ice, and snow. Also the entire image was studied using transparencies produced by the 3M color-key process. A combination of three complementary color combinations produced visually recognizable shades apparently indicating vegetation differences. Also lakes and glacial streams of sufficient size, ice and snow, and drainage patterns can be recognized. A need is indicated for oblique low level color aerial photography in the vicinity of identifiable terrain features to assist in the positive location of vegetative communities appearing in the analysis process. In the primary areas of concern to this project identifiable manmade features are conspicuous by their absence. In image 1049-20505 band 7 produced distinct tonal differences on a mountain slope to river bottom gradient.

Branton, C. I.↗
NASA NTRS1973

Identification, definition and mapping of terrestrial ecosystems in interior Alaska

The author has identified the following significant results. A reconstituted, simulated color-infrared print, enlarged to a scale of 1:250,000, was used to make a vegetation map of a 3,110 sq km area just west of Fairbanks, Alaska. Information was traced from the print which comprised the southeastern part of ERTS-1 scene 1033-21011. A 1:1,000,000 scale color-infrared transparency of this scene, obtained from NASA, was used along side the print as an aid in recognizing colors, color intensities and blends, and mosaics of different colors. Color units on the transparency and print were identified according to vegetation types using NASA air photos, U.S. Forest Service air photos, and experience of the investigator. Five more or less pure colors were identified and associated with vegetation types. These colors were designated according to their appearances on the print: (1) orange for forest vegetation dominated by broad-leaved trees: (2) gray for forest vegetation dominated by needle-leaved trees; (3) violet for scrub vegetation; (4) light violet denoting herbaceous tundra vegetation; and (5) dull violet for muskeg vegetation. This study has shown, through close examinations of the NASA transparency, that much more detailed vegetation landscape, or ecosystem maps could be produced, if only spectral signatures could be consistently and reliably recognized and transferred to a map of suitable scale.

Anderson, J. H.↗
NASA NTRS1973

Geologic mapping and mineral resource inventory by ERTS-1 satellite data in South America

The author has identified the following significant results. ERTS-1 data clearly provide significant contribution of new information on the remote areas of South America. Salar deposits have been measured and compared with those shown on World Navigation Charts; remarkable differences have been found in shape, size, number, and distribution. Repetitive coverage should enable us to develop an index of seasonal and annual environmental trends that can be compared with those of the Northern Hemisphere. New lineations, many of which are probably faults, have been found in Venezuela, Bolivia, and northern Argentina. Circular features, some of volcanic origin, have been recognized that are not shown on existing maps. The courses of several rivers have been revised and our Venezuelan counterparts report that a major new river has been recognized and charted. Large mining operations, such as the open pit copper mine of Chuquicamata in northern Chile, are recognizable and can be studied in their regional context.

Carter, W. D.↗
NASA NTRS1973

Lineaments in coastal plain sediments as seen in ERTS imagery

Examination of satellite imagery over the Atlantic Coastal Plain near Washington, D. C. shows numerous lineaments, which cannot be accounted for by any known cultural or natural features. At least some of these lineaments represent the surface expression of faults, for one of them has been correlated with the outcrop of a fault that had been traced for several miles in southern Prince Georges County, Maryland. If a substantial number of these lineaments do indeed represent fault traces, the fact that they show on the surface suggests that the geologic history of the Coastal Plain is much more complex than has previously been recognized, and that faulting may have occurred in the Holocene, much later than has generally been recognized. The importance that such recent movements could have on future development of the Coastal Plain should be emphasized.

Withington, C. F.↗
NASA NTRS1973

Sediment distribution and coastal processes in Cook Inlet, Alaska

Regional hydrologic and oceanographic relationships in Cook Inlet, Alaska have been recognized from sequential ERTS-1 MSS imagery. Current patterns are visible in the inlet because of differential concentrations of suspended sediment. The circulation patterns within Cook Inlet are controlled primarily by the interaction between the semi-diurnal tides and the counter clockwise Alaska current. In general, heavily sediment laden water is seen to be confined to portions of the inlet north of the Forelands and west of Kalgin Island. Tongues of clear oceanic water are observed to enter the inlet through Kennedy Channel along the east shoreline in the vicinity of Cape Elizabeth. A recurring counterclockwise circulation pattern observed around Kalgin Island seems to result from the interplay of the northerly moving water along the east shore and the southerly moving, sediment laden, water along the west side of the inlet. Prominent, fresh water plumes, heavily laden with sediment are visible at the mouths of all major rivers. Relect plumes from as many as three tidal stages have been recognized.

Anderson, D. M.↗
NASA NTRS1974

Geologic hypotheses of Lake Tanganyika region, Zaire, drawn from ERTS imagery

Based on initial work in the Lake Tanganyika area of eastern Zaire, it has been concluded that ERTS imagery is extremely useful for reconnaissance level geologic mapping and analysis in this region of the humid tropics. In particular, ERTS imagery has proven useful for recognizing and mapping regional structural units, for recognizing major structural features, and for arriving at some preliminary hypotheses about the mineral potential of the area. Results so far indicate that ERTS imagery can make a major contribution to the development of the mineral resources of the country. Research has concentrated on applications of ERTS imagery in the field of cartography, geology, forestry, hydrology and agriculture. For the work in geology, a test site was chosen in eastern Zaire on the shore of Lake Tanganyika in the vicinity of the Lukuga River. This area was selected because of its varied geology and the existence of two frames of cloud-free ERTS imagery.

Wolyce, U.↗