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At least 343 records · Page 19

Characterization of Treefoil Peptide Genes in Iron-Ion or X-Irradiated Human Cells

The gastrointestinal (GI) tract is especially sensitive to ionizing radiation, probably because of its high rate of cell turn over. Most of the data in the literature concerns the histological/anatomical description of damage rather than functional studies. In fact, previous reports in humans have shown that, at doses of 2 Gy or more, functional abnormalities appear indicating that in radiation sensitive tissues the effects of radiation are not limited to cell death. GI functions are controlled in particular by GI peptides. One hypothesis is that ionizing radiation may modulate the synthesis and release of these peptides and consequently may contribute largely to abnormalities in GI function. However, no previous studies have been concerned with GI-specific gene expression in irradiated GI tissues. The family of human trefoil peptides comprises three members thus far, all of which are expressed in specific regions of the GI tract. In addition, two trefoil peptides, pS2 (TFFI) and HITF (TFF2) are expressed in breast tissue. Their exact function in GI and breast tissues is unclear but mucosal integrity, repair, mucin secretion and responsiveness to hormones have been shown. We recently isolated and characterized pS2 as a novel p53- and estrogen receptor-independent gene whose MRNA expression in several cells lines was found to be delayed 4 to 7 days after irradiation with X-rays, fission neutrons or 1 GeV/n Fe-ions. The aim of the present study was to determine whether pS2 and HITF have a similar induction kinetics in irradiated gastric and breast cell lines, and whether they have the phorbol ester (TPA) responsive element (TRE).

Balcer-Kubiczek, E. K.↗

Simulation Study of Effects of Solar Irradiance and Sea Surface Temperature on Monsoons and Global Circulation

A recent version of the GEOS 2 GCM was used to isolate the roles of the annual cycles of solar irradiation and/or sea-surface temperatures (SSTs) on the simulated circulation and rainfall. Four 4-year long integrations were generated with the GCM. The first integration, called Control Case, used daily-interpolated SSTs from a 30 year monthly SST climatology that was obtained from the analyzed SST-data, while the solar irradiation at the top of the atmosphere was calculated normally at hourly intervals. The next two cases prescribed the SSTs or the incoming solar irradiance at the top of the atmosphere at their annual mean values, respectively while everything else was kept the same as in the Control Case. In this way the influence of the annual cycles of both external forcings was isolated.

Sud, Y. C.↗

Quantitative Assessment of the Integrated Response in Global Heat and Moisture Budgets to Changing Solar Irradiance

Earlier, we found time sequences of basin- and global-average upper ocean temperature (that is, diabatic heat storage above the main pycnocline) for 40 years from 1955-1994 and of sea surface temperature for 95 years from 1900-1994 associated with changes in the Sun's radiative forcing on decadal and interdecadal timescales, lagging by 10 deg.- 30 deg. of phase and confined to the upper 60-120 m. Yet, the observed changes in upper ocean temperature (approx. 0.1 K) were approximately twice those expected from the Stefan-Boltzmann black-body radiation law for the Earth's surface, with phase lags (0 deg. to 30 deg. of phase) much shorter than the 90 deg. phase shift expected as well. Moreover, White et al. (1997, 1998) found the Earth's global decadal mode in covarying SST and SLP anomalies phase locked to the decadal signal in the Sun's irradiance. Yet, Allan (2000) found this decadal signal also characterized by patterns similar to those observed on biennial and interannual time scales; that is, the Troposphere Biennial Oscillation (TBO) and the El Nino and the Southern Oscillation (ENSO). This suggested that small changes in the Sun's total irradiance could excite this global decadal mode in the Earth's ocean-atmosphere-terrestrial system similar to those excited internally on biennial and interannual period scales. This is a significant finding, proving that energy budget models (that is, models based on globally-averaged radiation balances) yield unrealistic responses. Thus, the true response must include positive and negative feedbacks in the Earth's ocean-atmosphere-terrestrial system as its internal mode (that is, the natural mode of the system) respond in damped resonance to quasi-periodic decadal changes in the Sun's irradiance. Moreover, these responses are not much different from those occurring internally on biennial and interannual period scales.

White, Warren B.↗

Investigation of the Relative Value of Solar Proxies for Irradiance Variation

Recent space observations of irradiance change during the current solar cycle 23 suggest that of the three proxies currently used (PSI index, total solar magnetic field, and the Mg II c/w index) only the latter yields a consistent result when compared to the two previous cycles for which space observations are available. Possible reasons for this are presented, and a case is made that using actual sunspot areas might work almost as well as the PSI index for estimating irradiance changes, making earlier observations lacking photometric calibration of value in assessing probable irradiance changes over earlier sunspot cycles.

Jordan, Stuart↗

Luminescence from VUV Irradiated Cosmic Ice Analogs and Organic Residues

The optical luminescent properties for a variety of vacuum-ultraviolet (VUV) irradiated cosmic ice analogs and the complex organic residues produced from irradiation might be applicable to Solar System and interstellar observations and processes for various astronomical objects with an ice heritage. Some examples include grain temperature determination and vaporization rates, nebula radiation balance, albedo values, color analysis, and biomarker identification. Detailed results are presented for the mixed molecular ice: H2O:CH3OH:NH3:CO (100:50:1:1), a realistic representation for an interstellar/precometary ice. The irradiated ices and the room-temperature residues resulting from this energetic processing have remarkable photoluminescent properties in the visible (520-570 nm). The luminescence dependence on temperature, thermal cycling, and VUV exposure of the residue is described.

Gudipati, Murthy S.↗

Measured and Simulated Dark J-V Characteristics of a-Si:H Single Junction p-i-n Solar Cells Irradiated with 40 keV Electrons

The effect of 40 keV electron irradiation on a-Si:H p-i-n single-junction solar cells was investigated using measured and simulated dark J-V characteristics. EPRI-AMPS and PC-1D simulators were explored for use in the studies. The EPRI-AMPS simulator was employed and simulator parameters selected to produce agreement with measured J-V characteristics. Three current mechanisms were evident in the measured dark J-V characteristics after electron irradiation, namely, injection, shunting and a term of the form CV(sup m). Using a single discrete defect state level at the center of the band gap, good agreement was achieved between measured and simulated J-V characteristics in the forward-bias voltage region where the dark current density was dominated by injection. The current mechanism of the form CV(sup m) was removed by annealing for two hours at 140 C. Subsequent irradiation restored the CV(sup m) current mechanism and it was removed by a second anneal. Some evidence of the CV(sup m) term is present in device simulations with a higher level of discrete density of states located at the center of the bandgap.

Lord, Kenneth↗

Luminescence from Vacuum-Ultraviolet-Irradiated Cosmic Ice Analogs and Residue

Here we report a study of the optical luminescent properties for a variety of vacuum-ultraviolet (VUV)-irradiated cosmic ice analogs and the complex organic residues produced. Detailed results are presented for the irradiated, mixed molecular ice: H2O: CH3OH:NH3:CO(100:50:1:1), a realistic representation for an interstellar/precometary ice that reproduces all the salient infrared spectral features associated with interstellar ices. The irradiated ices and the room-temperature residues resulting from this energetic processing have remarkable photoluminescent properties in the visible (520-570 nm). The luminescence dependence on temperature, thermal cycling, and VUV exposure is described. It is suggested that this type of luminescent behavior might be applicable to solar system and interstellar observations and processes for various astronomical objects with an ice heritage. Some examples include grain temperature determination and vaporization rates, nebula radiation balance, albedo values, color analysis, and biomarker identification.

Gudipati, Murthy S.↗

Luminescence from Vacuum-Ultraviolet-Irradiated Cosmic Ice Analogs and Residues

Here we report a study of the optical luminescent properties for a variety of vacuum-ultraviolet (VUV)- irradiated cosmic ice analogs and the complex organic residues produced. Detailed results are presented for the irradiated, mixed molecular ice: H2O:CH3OH:NH3:CO (100:50:1:1), a realistic representation for an interstellar/precometary ice that reproduces all the salient infrared spectral features associated with interstellar ices. The irradiated ices and the room-temperature residues resulting from this energetic processing have remarkable photoluminescent properties in the visible (520-570 nm). The luminescence dependence on temperature, thermal cycling, and VUV exposure is described. It is suggested that this type of luminescent behavior might be applicable to solar system and interstellar observations and processes for various astronomical objects with an ice heritage. Some examples include grain temperature determination and vaporization rates, nebula radiation balance, albedo values, color analysis, and biomarker identification.

Gudipati, Murthy S.↗

Evaluating Ground-based Proxies for Solar Irradiance Variation

In order to determine what ground-based proxies are best for evaluating solar irradiance variation before the advent of space observations, it is necessary to test these proxies against space observations. We have tested sunspot number, total sunspot area, and sunspot umbral area against the Nimbus-7 measurements of total solar irradiance variation cover the eleven year period 1980-1990. The umbral area yields the best correlation and the total sunspot area yields the poorest. Reasons for expecting the umbral area to yield the best correlation are given, the statistical procedure followed to obtain the results is described, and the value of determining the best proxy is discussed. The latter is based upon the availability of an excellent database from the Greenwich Observatory obtained over the period 1876-1976, which can be used to estimate the total solar irradiance variation before sensitive space observations were available. The ground-based observations used were obtained at the Coimbra Solar Observatory. The analysis was done at Goddard using these data and data from the Nimbus-7 satellite.

Oegerle, William↗

Observations of Solar Spectral Irradiance Change During Cycle 22 from NOAA-9 SBUV/2

The NOM-9 Solar Backscatter Ultraviolet, model 2 (SBUV/2) instrument is one of a series of instruments providing daily solar spectral irradiance measurements in the middle and near ultraviolet since 1978. The SBUV/2 instruments are primarily designed to measure stratospheric profile and total column ozone, using the directional albedo as the input to the ozone processing algorithm. As a result, the SBUV/2 instrument does not have onboard monitoring of all time-dependent response changes. We have applied internal comparisons and vicarious (external) comparisons to determine the long-term instrument characterization for NOAA-9 SBUV/2 to derive accurate solar spectral irradiances from March 1985 to May 1997 spanning two solar cycle minima with a single instrument. The NOAA-9 data show an amplitude of 9.3(+/- 2.3)% (81-day averaged) at 200-205 nm for solar cycle 22. This is consistent with the result of (Delta)F(sub 200-205) = 8.3(+/- 2.6)% for cycle 21 from Nimbus-7 SBUV and (Delta)F(sub 200-205) = 10(+/- 2)% (daily values) for cycle 23 from UARS SUSIM. NOAA-9 data at 245-250 nm show a solar cycle amplitude of (Delta)F(sub 245-250) = 5.7(+/- 1.8)%. NOAA-9 SBUV/2 data can be combined with other instruments to create a 25-year record of solar UV irradiance.

DeLand, Matthew T.↗

Laboratory Simulation of Space Weathering: ESR Measurements of Nanophase Metallic Iron in Laser-irradiated Olivine and Pyroxene Samples

S-type asteroids are believed to be parent bodies of ordinary chondrites. Although both S-type asteroids and ordinary chondrites contain the same mineral assemblage, mainly olivine and pyroxene, the reflectance spectra of the asteroids exhibit more overall depletion (darkening) and reddening, and more weakening of absorption bands relative to the meteorites. This spectral mismatch is explained by space weathering process, where high-velocity dust particle impacts should change the optical properties of the uppermost regolith surface of asteroids. In order to simulate the space weathering, we irradiated nanosecond pulse laser beam onto pellet samples of olivine (8.97wt% FeO) and pyroxene (enstatite: 9.88wt% FeO, hypersthene: 16.70wt%). We got spectral changes in our samples similar to that by space weathering on asteroids and confirmed nanophase alpha-metallic iron particles, which were theoretically predicted, not only on olivine but also on pyroxene samples by Transmission Electron Microscopy (TEM). Nanophase metallic iron particles were widely scattered throughout the amorphous rims developed along the olivine grains, whereas they were embedded in aggregates of amorphous in enstatite samples. Recently, we also measured laser-irradiated samples by ESR (Electron Spin Resonance). Strong ESR signals, characteristic to nanophase iron particles, are observed on irradiated olivine samples. In this paper, we report the quantities of nanophase metallic iron particles in pyroxene samples by ESR observations in addition to olivine samples.

Kurahashi, E.↗

Multivariate Analysis of Solar Spectral Irradiance Measurements

Principal component analysis is used to characterize approximately 7000 downwelling solar irradiance spectra retrieved at the Southern Great Plains site during an Atmospheric Radiation Measurement (ARM) shortwave intensive operating period. This analysis technique has proven to be very effective in reducing a large set of variables into a much smaller set of independent variables while retaining the information content. It is used to determine the minimum number of parameters necessary to characterize atmospheric spectral irradiance or the dimensionality of atmospheric variability. It was found that well over 99% of the spectral information was contained in the first six mutually orthogonal linear combinations of the observed variables (flux at various wavelengths). Rotation of the principal components was effective in separating various components by their independent physical influences. The majority of the variability in the downwelling solar irradiance (380-1000 nm) was explained by the following fundamental atmospheric parameters (in order of their importance): cloud scattering, water vapor absorption, molecular scattering, and ozone absorption. In contrast to what has been proposed as a resolution to a clear-sky absorption anomaly, no unexpected gaseous absorption signature was found in any of the significant components.

Pilewskie, P.↗

Limitation of Ground-based Estimates of Solar Irradiance Due to Atmospheric Variations

The uncertainty in ground-based estimates of solar irradiance is quantitatively related to the temporal variability of the atmosphere's optical thickness. The upper and lower bounds of the accuracy of estimates using the Langley Plot technique are proportional to the standard deviation of aerosol optical thickness (approx. +/- 13 sigma(delta tau)). The estimates of spectral solar irradiance (SSI) in two Cimel sun photometer channels from the Mauna Loa site of AERONET are compared with satellite observations from SOLSTICE (Solar Stellar Irradiance Comparison Experiment) on UARS (Upper Atmospheric Research Satellite) for almost two years of data. The true solar variations related to the 27-day solar rotation cycle observed from SOLSTICE are about 0.15% at the two sun photometer channels. The variability in ground-based estimates is statistically one order of magnitude larger. Even though about 30% of these estimates from all Level 2.0 Cimel data fall within the 0.4 to approx. 0.5% variation level, ground-based estimates are not able to capture the 27-day solar variation observed from SOLSTICE.

Wen, Guoyong↗

Quantification of rat retinal growth and vascular population changes after single and split doses of proton irradiation: translational study using stereology methods

This study quantified architectural and population changes in the rat retinal vasculature after proton irradiation using stereology. A 100 MeV conformal proton beam delivered 8, 14, 20 and 28 Gy as single and split doses to the whole eye. The vascular networks were prepared from retinal digests. Stereological methods were used to obtain the area of the retina and unbiased estimates of microvessel/artery/vein endothelial, pericyte and smooth muscle population, and vessel length. The retinal area increased progressively in the unirradiated, age-matched controls and in the retinas irradiated with 8 and 14 Gy, indicating uniform progressive retinal growth. No growth occurred after 20 and 28 Gy. Regression analysis of total endothelial cell number in all vessels (arteries, veins and capillaries) after irradiation documented a progressive time- and dose-dependent cell loss occurring over 15 to 24 months. The difference from controls was significant (P<0.01) after 28 Gy given in single and split doses and after 20 Gy given as a split dose (P<0.05). Total vessel length in microvessel was significantly shortened at 20 and 28 Gy compared to that of controls (P<0.05). No evident dose recovery was observed in the endothelial populations after split doses. At 10 Gy, the rate of endothelial cell loss, a dose parameter used to characterize the time- and dose-dependent loss of the endothelial population, was doubled.

NASA Discipline Radiation Health↗

Extrapolation of the dna fragment-size distribution after high-dose irradiation to predict effects at low doses

The patterns of DSBs induced in the genome are different for sparsely and densely ionizing radiations: In the former case, the patterns are well described by a random-breakage model; in the latter, a more sophisticated tool is needed. We used a Monte Carlo algorithm with a random-walk geometry of chromatin, and a track structure defined by the radial distribution of energy deposition from an incident ion, to fit the PFGE data for fragment-size distribution after high-dose irradiation. These fits determined the unknown parameters of the model, enabling the extrapolation of data for high-dose irradiation to the low doses that are relevant for NASA space radiation research. The randomly-located-clusters formalism was used to speed the simulations. It was shown that only one adjustable parameter, Q, the track efficiency parameter, was necessary to predict DNA fragment sizes for wide ranges of doses. This parameter was determined for a variety of radiations and LETs and was used to predict the DSB patterns at the HPRT locus of the human X chromosome after low-dose irradiation. It was found that high-LET radiation would be more likely than low-LET radiation to induce additional DSBs within the HPRT gene if this gene already contained one DSB.

NASA Discipline Radiation Health↗

Low irradiances affect abscisic acid, indole-3-acidic acid, and cytokinin levels of wheat (Triticum aestivum L.) tissues

Wheat (Triticum aestivum L.) plants were grown under four irradiance levels: 1,400, 400, 200, and 100 micromol m-2 s-1. Leaves and roots were sampled before, during, and after the boot stage, and levels of abscisic acid (ABA), indole-3-acetic acid (IAA), zeatin, zeatin riboside, dihydrozeatin, dihydrozeatin riboside, isopentenyl adenine, and isopentenyl adenosine were quantified using noncompetitive indirect ELISA systems. Levels of IAA in leaves and roots of plants exposed to 100 micromol m-2 s-1 of irradiance were 0.7 and 2.9 micromol kg-1 dry mass (DM), respectively. These levels were 0.2 and 1.0 micromol kg-1 DM, respectively, when plants were exposed to 1,400 micromol m-2 s-1. Levels of ABA in leaves and roots of plants exposed to 100 micromol m-2 s-1 were 0.65 and 0.55 micromol kg-1 DM, respectively. They were 0.24 micromol kg-1 DM (both leaves and roots) when plants were exposed to 1,400 micromol m-2 s-1. Levels of isopentenyl adenosine in leaves (24.3 nmol kg-1 DM) and roots (29.9 nmol kg-1 DM) were not affected by differences in the irradiance regime. Similar values were obtained in a second experiment. Other cytokinins could not be detected (<10 nmol kg 1 DM) in either experiment with the sample sizes used (150-600 mg DM for roots and shoots, respectively).

Non-NASA Center↗

Irradiance and spectral distribution control system for controlled environment chambers

This paper describes a closed-loop control system for controlling the irradiance and spectral quality generated by fluorescent lamps in a controlled environment chamber. The 400 to 800 nm irradiance and the ratio of the red waveband (600 to 700 nm) to the far-red waveband (700 to 800 nm) were independently controlled and varied as functions of time. A suggested application is to investigate the possibility of synergistic effects of changing irradiance levels and changing spectral distributions on photoperiodism and photomorphogenesis.

NASA Center KSC↗

Estimations of the Global Distribution and Time Series of UV Noontime Irradiance (305, 310, 324, 380 nm, and Erythemal) from TOMS and SeaWiFS Data

The amount of UV irradiance reaching the Earth's surface is estimated from the measured cloud reflectivity, ozone, aerosol amounts, and surface reflectivity time series from 1980 to 1992 and 1997 to 2000 to estimate changes that have occurred over a 21-year period. Recent analysis of the TOMS data shows that there has been an apparent increase in reflectivity (decrease in W) in the Southern Hemisphere that is related to a calibration error in EP-TOMS. Data from the well-calibrated SeaWiFS satellite instrument have been used to correct the EP-TOMS reflectivity and UV time series. After correction, some of the local trend features seen in the N7 time series (1980 to 1992) have been continued in the combined time series, but the overall zonal average and global trends have changed. In addition to correcting the EP-TOMS radiance calibration, the use of SeaWiFS cloud data permits estimation of UV irradiance at higher spatial resolution (1 to 4 km) than is available from TOMS (100 km) under the assumption that ozone is slowly varying over a scale of 100 km. The key results include a continuing decrease in cloud cover over Europe and North America with a corresponding increase in UV and a decrease in UV irradiance near Antarctica.

Herman, J.↗