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At least 343 records · Page 19

Anomaly Detection in Liquid Sodium Cold Trap Operation with Multisensory Data Fusion Using Long Short-Term Memory Autoencoder

Sodium-cooled fast reactors (SFR), which use high temperature fluid near ambient pressure as coolant, are one of the most promising types of GEN IV reactors. One of the unique challenges of SFR operation is purification of high temperature liquid sodium with a cold trap to prevent corrosion and obstructing small orifices. We have developed a deep learning long short-term memory (LSTM) autoencoder for continuous monitoring of a cold trap and detection of operational anomaly. Transient data were obtained from the Mechanisms Engineering Test Loop (METL) liquid sodium facility at Argonne National Laboratory. The cold trap purification at METL is monitored with 31 variables, which are sensors measuring fluid temperatures, pressures and flow rates, and controller signals. Loss-of-coolant type anomaly in the cold trap operation was generated by temporarily choking one of the blowers, which resulted in temperature and flow rate spikes. The input layer of the autoencoder consisted of all the variables involved in monitoring the cold trap. The LSTM autoencoder was trained on the data corresponding to cold trap startup and normal operation regime, with the loss function calculated as the mean absolute error (MAE). The loss during training was determined to follow log-normal density distribution. During monitoring, we investigated a performance of the LSTM autoencoder for different loss threshold values, set at a progressively increasing number of standard deviations from the mean. The anomaly signal in the data was gradually attenuated, while preserving the noise of the original time series, so that the signal-to-noise ratio (SNR) averaged across all sensors decreased below unity. Results demonstrate detection of anomalies with sensor-averaged SNR < 1.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

Coherence Effects in Ring Laser Gyros

We predict the propagation of slow and fast light in two co-resonant coupled optical resonators. In coupled resonators, slow light can propagate without attenuation by a cancellation of absorption as a result of mode splitting and destructive interference, whereas transparent fast light propagation can be achieved by the assistance of gain and splitting of the intracavity resonances, which consequently change the dispersion from normal to anomalous. The effective steady-state response of coupled- resonators is derived using the temporal coupled-mode formalism, and the absorptive and dispersive responses are described. Specifically, the occurrence of slow light via coupled-resonator-induced transparency and gain-assisted fast light are discussed. Keywords: whispering gallery modes, optical resonators, slow light, fast light, superluminal pulse propagation, coupled optical resonators.

Smith, David D.↗

Evaluation of Variable-Depth Liner Configurations for Increased Broadband Noise Reduction

This paper explores the effects of variable-depth geometry on the amount of noise reduction that can be achieved with acoustic liners. Results for two variable-depth liners tested in the NASA Langley Grazing Flow Impedance Tube demonstrate significant broadband noise reduction. An impedance prediction model is combined with two propagation codes to predict corresponding sound pressure level profiles over the length of the Grazing Flow Impedance Tube. The comparison of measured and predicted sound pressure level profiles is sufficiently favorable to support use of these tools for investigation of a number of proposed variable-depth liner configurations. Predicted sound pressure level profiles for these proposed configurations reveal a number of interesting features. Liner orientation clearly affects the sound pressure level profile over the length of the liner, but the effect on the total attenuation is less pronounced. The axial extent of attenuation at an individual frequency continues well beyond the location where the liner depth is optimally tuned to the quarter-wavelength of that frequency. The sound pressure level profile is significantly affected by the way in which variable-depth segments are distributed over the length of the liner. Given the broadband noise reduction capability for these liner configurations, further development of impedance prediction models and propagation codes specifically tuned for this application is warranted.

Jones, M. G.↗

Mechanism of selective recognition of Lys48-linked polyubiquitin by macrocyclic peptide inhibitors of proteasomal degradation

Post-translational modification of proteins with polyubiquitin chains is a critical cellular signaling mechanism in eukaryotes with implications in various cellular states and processes. Unregulated ubiquitin-mediated protein degradation can be detrimental to cellular homeostasis, causing numerous diseases including cancers. Recently, macrocyclic peptides were developed that selectively target long Lysine-48-linked polyubiquitin chains (tetra-ubiquitin) to inhibit ubiquitin-proteasome system, leading to attenuation of tumor growth in vivo. However, structural determinants of the chain length and linkage selectivity by these cyclic peptides remained unclear. Here, we uncover the mechanism underlying cyclic peptide’s affinity and binding selectivity by combining X-ray crystallography, solution NMR, and biochemical studies. We found that the peptide engages three consecutive ubiquitins that form a ring around the peptide and determined requirements for preferential selection of a specific trimer moiety in longer polyubiquitin chains. The structural insights gained from this work will guide the development of next-generation cyclic peptides with enhanced anti-cancer activity.

59 BASIC BIOLOGICAL SCIENCES↗

Slow and Fast Light in Coupled Microresonators

We predict the propagation of slow and fast light in two co-resonant coupled optical resonators. In coupled resonators, slow light can propagate without attenuation by a cancellation of absorption as a result of mode splitting and destructive interference, whereas transparent fast light propagation can be achieved by the assistance of gain and splitting of the intracavity resonances, which consequently change the dispersion from normal to anomalous. The effective steady-state response of coupled-resonators is derived using the temporal coupled-mode formalism, and the absorptive and dispersive responses are described. Specifically, the occurrence of slow light via coupled-resonator-induced transparency and gain-assisted fast light are discussed.

Chang, Hongrok↗

Acoustic Issues in Human Spaceflight

NASA is concerned about acute effect of sound on crew performance on International Space Station (ISS), and is developing strategies to assess and reduce acute, chronic, and delayed effects of sound. High noise levels can cause headaches, irritation, fatigue, impaired sleep, headache, and tinnitus and have resulted in an inability to hear alarms. Speech intelligibility may be more impaired for crew understanding non-native language in a noisy environment. No hearing loss occurred, but significant effects on crew performance and communication occurred. Permanent Threshold Shifts (PTS) have not been observed in the US shuttle program. Russian specification for noise in spacecraft is 60 dBA (awake) and 50 dBA (asleep) while the U.S. noise specification on ISS is NC 50 (awake) and NC 40 (asleep) with a 85 dBA hazard limit. Background noise levels of ISS modules have measured 56-69 dBA. Treadmill exercise operations measure 77 dBA. Alarms are required to be 20 dBA above ambient. Hearing protection is recommended when noise exceeds 60 dB 24 hour Leq. Countermeasures include hearing protection and design/ engineering controls. Advanced composite materials with excellent low frequency attenuation properties could be applied as a barrier protection around noisy equipment, or used on personal protective equipment worn by the crew. Hearing protection countermeasures include foam ear inserts, passive muff headsets, and active noise reduction headsets. Oto-acoustic emissions (OAE) could be used to monitor effectiveness of hearing protection countermeasures and tailor hearing protection countermeasures to individual crewmembers. Micro-gravity, vibration, toxic fumes, air quality/composition, stress, temperature, physical exertion or some combination of the above factors may have interacted with moderate long-term noise exposure to cause significant hearing loss. Longitudinal studies will need to address what co-morbidity factors, such as radiation, toxicology, microgravity effects (fluid shift), aging, are involved with hearing loss.

Clark, Jonathan B.↗

A new approach to estimating rainwater content by radar using propagation differential phase shift

As microwaves propagate through rain, the rate of phase change with increasing distance is different depending upon whether the transmissions are polarized horizontally or vertically. This rate of change is the so-called specific propagation differential phase shift phi(sub DP). This paper demonstrates that at several frequencies and over a wide domain the ratio of phi(sub DP) to the rainwater content W is nearly linearly related to D(sub m), the mass-weighted mean drop size. An investigation of errors indicates that this new approach is likely to yield more accurate estimates of W than the other classical reflectivity factor Z, attenuation, or polarization techniques. The most accurate estimates of W are most likely at the highest frequency considered, 13.80 GHz. In lieu of such high-frequency measurements, these somewhat esoteric results are made more concrete through an analysis of 3-GHz radar measurements collected during the Convection and Precipitation Experiment in a tropical rainstorm in Florida. Among the principal advantages of using phi(sub DP) to measure rain are that an absolute calibration of the radar is no longer required and the estimates are decoupled from measurements of the radar reflectivity factor. Consequently, temporal and spatial structures of rain estimates do not simply mimic those of the reflectivity factor, as happens for classical estimation techniques using Z.

Jameson, A. R.↗

Statistical and Prediction modeling of the Ka Band Using Experimental Results from ACTS Propagation Terminals at 20.185 and 27.505 GHZ

With the increase in demand for wireless communication services, most of the operating frequency bands have become very congested. The increase of wireless costumers is only fractional contribution to this phenomenon. The demand for more services such as video streams and internet explorer which require a lot of band width has been a more significant contributor to the congestion in a communication system. One way to increase the amount of information or data per unit of time transmitted with in a wireless communication system is to use a higher radio frequency. However in spite the advantage available in the using higher frequency bands such as, the Ka-band, higher frequencies also implies short wavelengths. And shorter wavelengths are more susceptible to rain attenuation. Until the Advanced Communication Technology Satellite (ACTS) was launched, the Ka- band frequency was virtually unused - the majority of communication satellites operated in lower frequency bands called the C- and Ku- bands. Ka-band is desirable because its higher frequency allows wide bandwidth applications, smaller spacecraft and ground terminal components, and stronger signal strength. Since the Ka-band is a high frequency band, the millimeter wavelengths of the signals are easily degraded by rain. This problem known as rain fade or rain attenuation The Advanced Communication Technology Satellite (ACTS) propagation experiment has collected 5 years of Radio Frequency (RF) attenuation data from December 1993 to November 1997. The objective of my summer work is to help develop the statistics and prediction techniques that will help to better characterize the Ka Frequency band. The statistical analysis consists of seasonal and cumulative five-year attenuation statistics for the 20.2 and 27.5 GHz. The cumulative five-year results give the link outage that occurs for a given link margin. The experiment has seven ground station terminals that can be attributed to a unique rain zone climate. The locations are White Sands, NM, Tampa, Fly Clarksburg, MD, Norman, OK, Ft. Collins, COY Vancouver, BC, and Fairbanks, AK. The analysis will help us to develop and define specific parameters that will help system engineers develop the appropriated instrumentation and structure for a Ka-band wireless communication systems and networks.

Ogunwuyi, Oluwatosin O.↗

'Direct' Gas-Phase Metallicities, Stellar Properties, and Local Environments of Emission-Line Galaxies at Redshifts Below 0.90

Using deep narrow-band (NB) imaging and optical spectroscopy from the Keck telescope and the Multi Mirror Telescope (MMT), we identify a sample of 20 emission-line galaxies (ELGs) at z = 0.065-0.90 where the weak auroral emission line, [O iii] lambda4363, is detected at >=3sigma. These detections allow us to determine the gas-phase metallicity using the "direct" method. With electron temperature measurements, and dust attenuation corrections from Balmer decrements, we find that 4 of these low-mass galaxies are extremely metal-poor with 12+log(O/H) <= 7.65 or one-tenth solar. Our most metal-deficient galaxy has 12+log(O/H)= 7.24(+0.45 / −0.30) (95% confidence), similar to some of the lowest metallicity galaxies identified in the local universe. We find that our galaxies are all undergoing significant star formation with average specific star formation rate (SFR) of (100 Myra)(exp −1), and that they have high central SFR surface densities (average of 0.5 Solar M / yr/ sq. kpc). In addition, more than two-thirds of our galaxies have between one and four nearby companions within a projected radius of 100 kpc, which we find is an excess among star-forming galaxies at z =0.4 -- 0.85. We also find that the gas-phase metallicities for a given stellar mass and SFR lie systematically lower than the local stellar M-Z-(SFR) relation by approx. = 0.2 dex (2 sigma significance). These results are partly due to selection effects, since galaxies with strong star formation and low metallicity are more likely to yield [O iii] lambda4363 detections. Finally, the observed higher ionization parameter and high electron density suggest that they are lower redshift analogs to typical z approx. > 1 galaxies.

Direct↗

A Generalised Porosity Formalism for Isotropic and Anisotropic Effective Opacity and its Effects on X-ray Line Attenuation in Clumped O Star Winds

We present a generalised formalism for treating the porosity-associated reduction in continuum opacity that occurs when individual clumps in a stochastic medium become optically thick. As in previous work, we concentrate on developing bridging laws between the limits of optically thin and thick clumps. We consider geometries resulting in either isotropic or anisotropic effective opacity, and, in addition to an idealised model in which all clumps have the same local overdensity and scale, we also treat an ensemble of clumps with optical depths set by Markovian statistics. This formalism is then applied to the specific case of bound-free absorption of X- rays in hot star winds, a process not directly affected by clumping in the optically thin limit. We find that the Markov model gives surprisingly similar results to those found previously for the single clump model, suggesting that porous opacity is not very sensitive to details of the assumed clump distribution function. Further, an anisotropic effective opacity favours escape of X-rays emitted in the tangential direction (the venetian blind effect), resulting in a bump of higher flux close to line centre as compared to profiles computed from isotropic porosity models. We demonstrate how this characteristic line shape may be used to diagnose the clump geometry, and we confirm previous results that for optically thick clumping to significantly influence X-ray line profiles, very large porosity lengths, defined as the mean free path between clumps, are required. Moreover, we present the first X-ray line profiles computed directly from line-driven instability simulations using a 3-D patch method, and find that porosity effects from such models also are very small. This further supports the view that porosity has, at most, a marginal effect on X-ray line diagnostics in O stars, and therefore that these diagnostics do indeed provide a good clumping insensitive method for deriving O star mass-loss rates.

Sundqvist, Jon O.↗

A Generalised Porosity Formalism for Isotropic and Anisotropic Effective Opacity and Its Effects on X-ray Line Attenuation in Clumped O Star Winds

We present a generalised formalism for treating the porosity-associated reduction in continuum opacity that occurs when individual clumps in a stochastic medium become optically thick. As in previous work, we concentrate on developing bridging laws between the limits of optically thin and thick clumps. We consider geometries resulting in either isotropic or anisotropic effective opacity, and, in addition to an idealised model in which all clumps have the same local overdensity and scale, we also treat an ensemble of clumps with optical depths set by Markovian statistics. This formalism is then applied to the specific case of bound-free absorption of X- rays in hot star winds, a process not directly affected by clumping in the optically thin limit. We find that the Markov model gives surprisingly similar results to those found previously for the single clump model, suggesting that porous opacity is not very sensitive to details of the assumed clump distribution function. Further, an anisotropic effective opacity favours escape of X-rays emitted in the tangential direction (the venetian blind effect), resulting in a bump of higher flux close to line centre as compared to profiles computed from isotropic porosity models. We demonstrate how this characteristic line shape may be used to diagnose the clump geometry, and we confirm previous results that for optically thick clumping to significantly influence X-ray line profiles, very large porosity lengths, defined as the mean free path between clumps, are required. Moreover, we present the first X-ray line profiles computed directly from line-driven instability simulations using a 3-D patch method, and find that porosity effects from such models also are very small. This further supports the view that porosity has, at most, a marginal effect on X-ray line diagnostics in O stars, and therefore that these diagnostics do indeed provide a good clumping insensitive method for deriving O star mass-loss rates.

generalised↗

Digital Beamforming Scatterometer

This paper discusses scatterometer measurements collected with multi-mode Digital Beamforming Synthetic Aperture Radar (DBSAR) during the SMAP-VEX 2008 campaign. The 2008 SMAP Validation Experiment was conducted to address a number of specific questions related to the soil moisture retrieval algorithms. SMAP-VEX 2008 consisted on a series of aircraft-based.flights conducted on the Eastern Shore of Maryland and Delaware in the fall of 2008. Several other instruments participated in the campaign including the Passive Active L-Band System (PALS), the Marshall Airborne Polarimetric Imaging Radiometer (MAPIR), and the Global Positioning System Reflectometer (GPSR). This campaign was the first SMAP Validation Experiment. DBSAR is a multimode radar system developed at NASA/Goddard Space Flight Center that combines state-of-the-art radar technologies, on-board processing, and advances in signal processing techniques in order to enable new remote sensing capabilities applicable to Earth science and planetary applications [l]. The instrument can be configured to operate in scatterometer, Synthetic Aperture Radar (SAR), or altimeter mode. The system builds upon the L-band Imaging Scatterometer (LIS) developed as part of the RadSTAR program. The radar is a phased array system designed to fly on the NASA P3 aircraft. The instrument consists of a programmable waveform generator, eight transmit/receive (T/R) channels, a microstrip antenna, and a reconfigurable data acquisition and processor system. Each transmit channel incorporates a digital attenuator, and digital phase shifter that enables amplitude and phase modulation on transmit. The attenuators, phase shifters, and calibration switches are digitally controlled by the radar control card (RCC) on a pulse by pulse basis. The antenna is a corporate fed microstrip patch-array centered at 1.26 GHz with a 20 MHz bandwidth. Although only one feed is used with the present configuration, a provision was made for separate corporate feeds for vertical and horizontal polarization. System upgrades to dual polarization are currently under way. The DBSAR processor is a reconfigurable data acquisition and processor system capable of real-time, high-speed data processing. DBSAR uses an FPGA-based architecture to implement digitally down-conversion, in-phase and quadrature (I/Q) demodulation, and subsequent radar specific algorithms. The core of the processor board consists of an analog-to-digital (AID) section, three Altera Stratix field programmable gate arrays (FPGAs), an ARM microcontroller, several memory devices, and an Ethernet interface. The processor also interfaces with a navigation board consisting of a GPS and a MEMS gyro. The processor has been configured to operate in scatterometer, Synthetic Aperture Radar (SAR), and altimeter modes. All the modes are based on digital beamforming which is a digital process that generates the far-field beam patterns at various scan angles from voltages sampled in the antenna array. This technique allows steering the received beam and controlling its beam-width and side-lobe. Several beamforming techniques can be implemented each characterized by unique strengths and weaknesses, and each applicable to different measurement scenarios. In Scatterometer mode, the radar is capable to.generate a wide beam or scan a narrow beam on transmit, and to steer the received beam on processing while controlling its beamwidth and side-lobe level. Table I lists some important radar characteristics

Rincon, Rafael F.↗

Nucleation and Growth According to Lysozyme

How does one take a molecule, strongly asymmetric in both shape and charge distribution, and assemble it into a crystal? We propose a model for the nucleation and crystal growth process for tetragonal lysozyme that may be very germane to other monomeric proteins. The first species formed is postulated to be a dimer. Through repeating associations involving the same intermolecular interactions this becomes the 4(sub 3) helix, that in turn serves as the basic unit for nucleation and crystal growth. High salt attenuates surface charges while promoting hydrophobic interactions. Symmetry facilitates helix self-association. Assembly stability is enhanced when a four helix structure is obtained, with each bound to two neighbors. Only two unique interactions are required. The first are those for helix formation, where the dominant interaction is the intermolecular bridging anion. The second is the anti-parallel side-by-side helix-helix interaction, guided by alternating pairs of symmetry related salt bridges along each side. At this stage all eight unique positions of the P4(sub 3)2(sub 1)2(sub 1) unit cell are filled. From the above, the process is one of a) attenuating the most strongly interacting groups, such that b) the molecules begin to self-associate in defined patterns, so that c) symmetry is obtained, which d) propagates as a growing crystal. Simple and conceptually obvious in hindsight, this tells much about what we are empirically doing when we crystallize macromolecules. By adjusting the solution parameters we are empirically balancing the intermolecular interactions, preferentially attenuating the dominant strong (for lysozyme the charged groups) while strengthening the lesser strong (hydrophobic) interactions. Lysozyme is atypical in the breadth of its crystallization conditions; many proteins only crystallize under narrowly defined conditions, pointing to the criticality of the empirical balancing process. Lack of a singularly defined association pathway leads to formation of multiple species, i.e., amorphous precipitation. Weak interactions, such as hydrogen bonds, are promiscuous, serving to strengthen rather than define specific interactions. Participation in an interaction sequesters that surface from subsequent interactions, and we expect the strongest bonds to form first. When two molecules self associate the resulting species will have an axis of symmetry. Subsequent interactions between two associated species having equivalent interactions will also have symmetry. Only a few unique sets of interactions are required to give any of the commonly found space groups for monomeric proteins. This model and what it suggests will be discussed.

Pusey, Marc L.↗

A large blood pressure-raising effect of nitric oxide synthase inhibition in humans

In experimental animals, systemic administration of nitric oxide synthase (NOS) inhibitors causes large increases in blood pressure that are in part sympathetically mediated. The aim of this study was to determine the extent to which these conclusions can be extrapolated to humans. In healthy normotensive humans, we measured blood pressure in response to two NOS inhibitors, NG-monomethyl-L-arginine (L-NMMA) and NG-nitro-L-arginine methyl ester (L-NAME), the latter of which recently became available for use in humans. The major new findings are 3-fold. First, L-NAME produced robust increases in blood pressure that were more than 2 times larger than those previously reported in humans with L-NMMA and approximated those seen in experimental animals. L-NAME (4 mg/kg) raised mean arterial pressure by 24+/-2 mm Hg (n=27, P<0.001), whereas in subjects who received both inhibitors, a 12-fold higher dose of L-NMMA (50 mg/kg) raised mean arterial pressure by 15+/-2 mm Hg (n=4, P<0.05 vs L-NAME). Second, the L-NAME-induced increases in blood pressure were caused specifically by NOS inhibition because they were reversed by L-arginine (200 mg/kg, n=12) but not D-arginine (200 mg/kg, n=6) and because NG-nitro-D-arginine methyl ester (4 mg/kg, n=5) had no effect on blood pressure. Third, in humans, there is an important sympathetic component to the blood pressure-raising effect of NOS inhibition. alpha-Adrenergic blockade with phentolamine (0.2 mg/kg, n=9) attenuated the L-NAME-induced increase in blood pressure by 40% (P<0.05). From these data, we conclude that pharmacological inhibition of NOS causes large increases in blood pressure that are in part sympathetically mediated in humans as well as experimental animals.

Non-NASA Center↗

Statistically Based Approach to Broadband Liner Design and Assessment

A broadband liner design optimization includes utilizing in-duct attenuation predictions with a statistical fan source model to obtain optimum impedance spectra over a number of flow conditions for one or more liner locations in a bypass duct. The predicted optimum impedance information is then used with acoustic liner modeling tools to design liners having impedance spectra that most closely match the predicted optimum values. Design selection is based on an acceptance criterion that provides the ability to apply increasing weighting to specific frequencies and/or operating conditions. One or more broadband design approaches are utilized to produce a broadband liner that targets a full range of frequencies and operating conditions.

Nark, Douglas M.↗

Iodine Co-Precipitation with Calcium Carbonate in the Presence of Silica Ions - 20360

Historical releases of I-129 to the subsurface at the U.S. Department of Energy Hanford Site have resulted in large dilute plumes that cover an area of over 50 km{sup 2}. The most concentrated I-129 plume is associated with the 200 West Area in the 200-UP-1 operable unit of the Hanford Central Plateau, where peak concentrations have been measured up to 30 times the drinking water standard of 1 pCi/L. The mobility of iodine depends on many factors, including chemical speciation, pH, redox conditions, as well as the presence of organic matter, calcium carbonate minerals and microbial activities. Speciation measurements estimate that the majority of the iodine (∼76%) in Hanford groundwater exists as iodate. Information on processes that affect iodate mobility in the Hanford subsurface support evaluation of environmental management options for the I-129 plumes. Iodate can be incorporated into calcium carbonate, which is a potential mechanism of naturally attenuating radioiodine in groundwater. However, the silica content in porewater may impact the contaminant-calcium carbonate incorporation process. Silica is one of the most abundant elements in nature and can have an impact on chemical weathering of alkaline-earth carbonates in the environment. Hence, this research aims to advances knowledge of the iodine co-precipitation process with calcium carbonate in the presence of silica. This information supports the technical basis evaluation of natural attenuation for I-129. Samples for these studies were prepared using calcium carbonate-forming solutions that included certified grade sodium meta-silicate nonahydrate (Na{sub 2}SiO{sub 3}), calcium chloride dehydrate (CaCl{sub 2}.2H{sub 2}O), sodium carbonate (NaCO{sub 2}), and sodium hydroxide (NaOH) solutions for pH adjustments. Triplicate samples were prepared with silica concentrations in the solution of 0 mM, 0.5 mM and 20 mM, mixed with an iodate standard (1000 ppm of IO{sub 3}{sup -} in H{sub 2}O) and sampled over a one-week period. Preliminary data analyses suggest that less iodine remains in solution in the presence of silica (average remaining aqueous fraction 0.23±0.03 at 20 mM of Si) relative to samples without silica (0.52±0.06); an indication that more iodine has been sorbed or incorporated into precipitates. However, the mechanisms for this behavior are still under investigation and need to be interpreted relative to pore water compositions that are representative of Hanford site-specific conditions. Scanning electron microscope equipped with energy dispersive spectroscopy (SEM-EDS) analyses were performed to observe the solid phase morphology in these experiments and showed rhombohedral calcite crystals covered with amorphous Si floccules. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Mitoferrin 2 deficiency prevents mitochondrial iron overload-induced endothelial injury and alleviates atherosclerosis

Endothelial dysfunction is an early step in the development of atherosclerotic cardiovascular disease. Iron overload can lead to excessive mitochondrial reactive oxygen species (mtROS) production, resulting in mitochondrial dysfunction and vascular endothelial cell (EC) damage. Mitoferrin 2 (Mfrn2) is an iron transporter in the inner mitochondrial membrane. This study aimed to assess whether Mfrn2 and mitochondrial iron overload were involved in atherosclerosis progression and to explore the potential mechanism. We observed significant upregulation of Mfrn2 in the arteries of high-fat diet (HFD)-fed Apolipoprotein E{sup −/-} (ApoE{sup −/-}) mice and in TNF-α-induced mouse aortic endothelial cells (MAECs). Mfrn2 gene silencing inhibited mitochondrial iron overload, stabilized mitochondrial membrane potential and improved mitochondrial function in TNF-α-induced MAECs. Vascular EC-specific knockdown of Mfrn2 in ApoE{sup −/-} mice markedly decreased atherosclerotic lesion formation and the levels of ICAM-1 in aortas and reduced monocyte infiltration into the vascular wall. Furthermore, TNF-α increased the binding of 14-3-3 epsilon (ε) and Mfrn2, preventing Mfrn2 degradation and leading to mitochondrial iron overload in ECs, while 14-3-3ε overexpression increased Mfrn2 stability by inhibiting its ubiquitination. Together, our results reveal that Mfrn2 deficiency attenuates endothelial dysfunction by decreasing iron levels within the mitochondria and mitochondrial dysfunction. These findings may provide new insights into preventive and therapeutic strategies against vascular endothelial dysfunction in atherosclerotic disease.

60 APPLIED LIFE SCIENCES↗

Improved multilayer insulation applications

Multilayer insulation blankets used for the attenuation of radiant heat transfer in spacecraft are addressed. Typically, blanket effectiveness is degraded by heat leaks in the joints between adjacent blankets and by heat leaks caused by the blanket fastener system. An approach to blanket design based upon modular sub-blankets with distributed seams and upon an associated fastener system that practically eliminates the through-the-blanket conductive path is described. Test results are discussed providing confirmation of the approach. The specific case of the thermal control system for the optical assembly of the Space Telescope is examined.

Mikk, G.↗