Proportions and Serogroups of Enterohemorrhagic Shiga Toxin-producing Escherichia coli in Feces of Fed and Cull Beef and Cull Dairy Cattle at Harvest
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Biotechnology & Applied Microbiology↗
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Where's the Beaverhead beef?
Only rare quartz grains with single-set planar (1013) deformation features (PDF's) are present in breccia dikes found in association with uniformly oriented shatter cones that occur over an area 8 x 25 km. This suggests that the Beaverhead shocked rocks come from only the outer part of the central uplift of what must have been a large (greater than 100 km diameter) complex impact structure. An impact event of this magnitude on continental crust (thought to have occurred in late Precambrian or ealy Paleozoic time) could be expected to punctuate local geologic history. Furthermore, although it may now be covered, its scar should remain despite all the considerable subsequent erosion/deposition and tectonism since the impact. The following are three large-scale singularities or anomalies that may reflect the event and mark its source. (1) The Lemhi Arch is a major structural uplift that occurred in late Proterozoic-early Paleozoic time in East Central Idaho and caused the erosion of at least 4 km of sedimentary cover. This may be directly related to the impact. (2) Of the many thrust sheets comprising the Cordilleran belt, the Cabin plate that carries the shocked rocks is unique in that it alone intersected the crystalline basement. It also now marks the apex of the Southwest Montana Recess in the Sevier front. The basement uplift remaining from the impact may have constituted a mechanical obstacle to the advancing thrust sheets in Cretaceous time, causing the recess. (3) What could be interpreted as a roughly circular aeromagnetic anomaly approx. 70 km in diameter can be discerned in the state aeromagnetic map centered about 20 km southeast of Challis, Idaho, in the Lost River range. It is in approximately the right place, and ignoring the possibility that the anomalies have diverse causes and the circular pattern is coincidental, it may mark what remains of the buried central uplift structure.
Hargraves, R. B.↗
Here's the beef: A case study in organizational transformation
The Science and Technology Lab (STL) is tasked with the design, development, and application of the science and engineering services. Formed in the early 1970's STL adhered to many traditional attitudes including barriers to communication, excessive management control, parochial strategies, unclear measures of success, lack of customer focus, underutilization of people, and excessive administrative burdens on scientists and engineers. The challenge for the STL was to maximize customer satisfaction through the effective and efficient application of the notable skills and talents of the STL's workforce. In this way, the Lab would begin its exciting journey toward becoming world class. A discussion of this on-going transformation is presented.
Huseonica, William F.↗
Distributed parameter modelling of flexible spacecraft: Where's the beef?
This presentation discusses various misgivings concerning the directions and productivity of Distributed Parameter System (DPS) theory as applied to spacecraft vibration control. We try to show the need for greater cross-fertilization between DPS theorists and spacecraft control designers. We recommend a shift in research directions toward exploration of asymptotic frequency response characteristics of critical importance to control designers.
Hyland, D. C.↗
Membrane insertion for the detection of lipopolysaccharides: Exploring the dynamics of amphiphile-in-lipid assays
Shiga toxin-producing Escherichia coli is an important cause of foodborne illness, with cases attributable to beef, fresh produce and other sources. Many serotypes of the pathogen cause disease, and differentiating one serotype from another requires specific identification of the O antigen located on the lipopolysaccharide (LPS) molecule. The amphiphilic structure of LPS poses a challenge when using classical detection methods, which do not take into account its lipoglycan biochemistry. Typically, detection of LPS requires heat or chemical treatment of samples and relies on bioactivity assays for the conserved lipid A portion of the molecule. Our goal was to develop assays to facilitate the direct and discriminative detection of the entire LPS molecule and its O antigen in complex matrices using minimal sample processing. To perform serogroup identification of LPS, we used a method called membrane insertion on a waveguide biosensor, and tested three serogroups of LPS. The membrane insertion technique allows for the hydrophobic association of LPS with a lipid bilayer, where the exposed O antigen can be targeted for specific detection. Samples of beef lysate were spiked with LPS to perform O antigen specific detection of LPS from E. coli O157. To validate assay performance, we evaluated the biophysical interactions of LPS with lipid bilayers both in- and outside of a flow cell using fluorescence microscopy and fluorescently doped lipids. Our results indicate that membrane insertion allows for the qualitative and reliable identification of amphiphilic LPS in complex samples like beef homogenates. In addition, we also demonstrated that LPS-induced hole formation does not occur under the conditions of the membrane insertion assays. Together, these findings describe for the first time the serogroup-specific detection of amphiphilic LPS in complex samples using a membrane insertion assay, and highlight the importance of LPS molecular conformations in detection architectures.
59 BASIC BIOLOGICAL SCIENCES↗
Integrated energy-water-land nexus planning to guide national policy: an example from Uruguay
Despite broad consensus on the benefits of a nexus approach to multi-sector planning, actual implementation in government and other decision-making institutions is still rare. This study presents an approach to conducting integrated energy-water-land (EWL) planning, using Uruguay as an example. This stakeholder-driven study focuses on assessing the EWL nexus implications of actual planned policies aimed at strengthening three of Uruguay's key exports (beef, soy, and rice), which account for more than 40% of total national export revenue. Five scenarios are analyzed in the study: a reference scenario, a climate impacts scenario, and three policy scenarios. The three policy scenarios include measures such as increasing the intensity of beef production while simultaneously decreasing emissions, increasing irrigated soybean production, and improving rice yields. This study supplements previous sector-specific planning efforts in Uruguay by conducting the first stakeholder-driven integrated multi-sector assessment of planned policies in Uruguay using a suite of integrated modeling tools. Key insights from the study are: as compared to a reference scenario, improving beef productivity could lead to cropland expansion (+30%) and significant indirect increases in water requirements (+20%); improving rice yields could lead to increases in total emissions (+3%), which may partially offset emissions reductions from other policies; expanding irrigated soy could have the least EWL impacts amongst the policies studied; and climate-driven changes could have significantly less impact on EWL systems as compared to human actions. The generalizable insights derived from this analysis are readily applicable to other countries facing similar multi-sector planning challenges. In particular, the study's results reinforce the fact that policies often have multi-sector consequences, and thus policies can impact one another's efficacy. Thus, policy design and implementation can benefit from coordination across sectors and decision-making institutions.
54 ENVIRONMENTAL SCIENCES↗
Sensitive detection of Escherichia coli O157:H7 in food and water by immunomagnetic separation and solid-phase laser cytometry
Rapid, direct methods are needed to assess active bacterial populations in water and foods. Our objective was to determine the efficiency of bacterial detection by immunomagnetic separation (IMS) and the compatibility of IMS with cyanoditolyl tetrazolium chloride (CTC) incubation to determine respiratory activity, using the pathogen Escherichia coli O157:H7. Counterstaining with a specific fluorescein-conjugated anti-O157 antibody (FAb) following CTC incubation was used to allow confirmation and visualization of bacteria by epifluorescence microscopy. Broth-grown E. coli O157:H7 was used to inoculate fresh ground beef (<17% fat), sterile 0.1% peptone, or water. Inoculated meat was diluted and homogenized in a stomacher and then incubated with paramagnetic beads coated with anti-O157 specific antibody. After IMS, cells with magnetic beads attached were stained with CTC and then an anti-O157 antibody-fluorescein isothiocyanate conjugate and filtered for microscopic enumeration or solid-phase laser cytometry. Enumeration by laser scanning permitted detection of ca. 10 CFU/g of ground beef or <10 CFU/ml of liquid sample. With inoculated meat, the regression results for log-transformed respiring FAb-positive counts of cells recovered on beads versus sorbitol-negative plate counts in the inoculum were as follows: intercept = 1.06, slope = 0.89, and r2 = 0. 95 (n = 13). The corresponding results for inoculated peptone were as follows: intercept = 0.67, slope = 0.88, and r2 = 0.98 (n = 24). Recovery of target bacteria on beads by the IMS-CTC-FAb method, compared with recovery by sorbitol MacConkey agar plating, yielded greater numbers (beef, 6.0 times; peptone, 3.0 times; water, 2.4 times). Thus, within 5 to 7 h, the IMS-CTC-FAb method detected greater numbers of E. coli O157 cells than were detected by plating. The results show that the IMS-CTC-FAb technique with enumeration by either fluorescence microscopy or solid-phase laser scanning cytometry gave results that compared favorably with plating following IMS.
Non-NASA Center↗
Interactive effects of precipitation, temperature, and grazing pressure on the net ecosystem carbon balance of grazing lands across the continental United States
Grazing lands cover approximately one-third of the contiguous United States, support much of the nation's beef production, and are an important component of the U.S. terrestrial carbon budget. In this study, we quantified net ecosystem carbon balance (NECB), the net status of grazing lands as a carbon sink (C-sink) or source (C-source) and a key determinant of soil health and productivity. Our primary objective was to synthesize multiple years of annual NECB across heterogeneous grazing lands across the continental U.S and evaluate annual NECB against physical drivers (mean annual precipitation (MAP), mean annual temperature (MAT), vegetation, and moisture condition) and management practices (grazing pressure index (GPI) and fertilization history). We hypothesized that (1) NECB is higher in mesic and fertilized grasslands; (2) NECB increases with MAP and MAT but decreases with GPI; and (3) interactive effects exist among MAP, MAT, and GPI. Using carbon fluxes measured by eddy covariance towers and methane emissions including both enteric methane and manure derived from stocking rates across seven USDA Long-term Agroecosystem Research Network sites, we found: (1) grazing lands were a C-sink or carbon neutral at most sites; (2) vegetation type, moisture conditions, or fertilization had no significant effect on NECB; (3) NECB increased with MAP and MAT, but decreased with a higher GPI; and (4) MAT had a significant positive effect on NECB when MAP exceeded 750 mm (greater water availability). The effect of GPI on NECB was significantly negative when MAP was below 1000 mm, significantly negative when MAT < 12 °C and significantly positive when MAT > 16 °C. Thus, most grazing lands in our study acted as C-sinks unless water deficit, low temperature, or heavy grazing were interactively present. Understanding how climate and management influence NECB of grazing lands is key to maintaining resilient agroecosystems that secure beef production and sustain rural prosperity.
LTAR↗
Purification and characterization of lipopolysaccharides from six strains of non-O157 Shiga toxin-producing Escherichia coli
Certain Shiga toxin-producing Escherichia coli (STEC) are virulent human pathogens that are most often acquired through contaminated food. The United States Department of Agriculture, Food Safety and Inspection Service has declared several serogroups of STEC as adulterants in non-intact raw beef products. Hence, sensitive and specific tests for the detection of these STEC are a necessity for implementation in food safety programs. E. coli serogroups are identified by their respective O-antigen moiety on the lipopolysaccharide (LPS) macromolecule. We propose that the development of O-antigen-specific immunological assays can facilitate simple and rapid discriminatory detection of STEC in beef. However, the resources (antigens and antibodies) required for such development are not readily available. To overcome this, we extracted and characterized LPS and O-antigen from six STEC strains. Using hot phenol extraction, we isolated the LPS component from each strain and purified it using a series of steps to eliminate proteins, nucleic acids, and lipid A antigens. Antigens and crude LPS extracts were characterized using gel electrophoresis, immunoblotting, and modified Western blotting with commercially available antibodies, thus assessing the serogroup specificity and sensitivity of available ligands as well. The results indicate that, while many commercially available antibodies bind LPS, their activities and specificities are highly variable, and often not as specific as those required for serogroup discrimination. This variability could be minimized by the production of antibodies specific for the O-antigen. Additionally, the antigens generated from this study provide a source of characterized LPS and O-antigen standards for six serogroups of STEC.
59 BASIC BIOLOGICAL SCIENCES↗
A comparative analysis of different van der Waals treatments for molecular adsorption on the basal plane of 2H-MoS 2
The binding energy of hydrogen sulfide, ammonia, ethane, ethylene, butadiene, benzene, toluene, pyridine, pyrrole, and thiophene on the basal plane of the semi-conducting 2H-molybdenum sulfide (MoS 2 ) was calculated with the following flavors of Density Functional Theory (DFT): GGA-PW91, PBE-D3, vdW-DF, optPBE, optB86b, optB88, vdW-TS, and BEEF-vdW. The GGA-PW91 binding energies are negligible (<0.07 eV in magnitude) in all cases. The predictions with vdW-DF and PBE-D3 are the closest (error <0.05 eV) to the isosteric heats of adsorption calculated from reported temperature programmed desorption data for thiophene and butadiene. For all dispersion flavors examined here, the magnitude of the dispersion contribution to the binding energy increases linearly with the number of heavy atoms in the adsorbate, with each atom contributing 0.05 eV (BEEF-vdW) – 0.09 eV (optB88-vdW). Further, this implies that the calculated adsorption constants of molecules larger than acridine (i.e., comprising > 14 non-heavy atoms) can vary by more than four orders of magnitude at industrial conditions depending on the chosen method of dispersion correction. Further, dispersion effects fall off rapidly (>0.03 eV/ non-hydrogen atom/Å) as the adsorbate-surface distance increases.
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
Validation of Density Functionals for Adsorption Energies on Transition Metal Surfaces
The quantitative prediction of adsorption energies of radicals and molecules on surfaces is essential for the design and understanding of heterogeneous catalytic processes. Here, a recent paper by Wellendorff et al. collected an experimental database of 39 reaction energies involving adsorption energies on transition metal surfaces that can be used as benchmarks for testing quantum mechanical electronic structure methods, and we compared the experimental data to Kohn–Sham density functional calculations with six exchange–correlation functionals. In this paper, we rearranged the data into two categories: open-shell radical adsorption reactions and closed-shell molecular adsorption reactions. We recalculated the adsorption energies with PBE, and we also calculated them with three functionals, M06-L, GAM, and MN 15 -L, that were not studied in the Wellendorff et al. paper; then we compared our results to the benchmark data. Of the nine functionals that have been compared to the databases, we find that BEEF-vdW, GAM, and RPBE perform best for the open-shell radical adsorption reactions, and MN15-L performs best for the closed-shell molecular adsorption, followed by BEEF-vdW and M06-L.
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
Intrinsic selectivity and structure sensitivity of Rhodium catalysts for C 2+ oxygenate production [On the intrinsic selectivity and structure sensitivity of Rhodium catalysts for C 2+ oxygenate production]
Synthesis gas (CO + H 2 ) conversion is a promising route to converting coal, natural gas, or biomass into synthetic liquid fuels. Rhodium has long been studied as it is the only elemental catalyst that has demonstrated selectivity to ethanol and other C 2+ oxygenates. However, the fundamentals of syngas conversion over rhodium are still debated. In this work a microkinetic model is developed for conversion of CO and H 2 into methane, ethanol, and acetaldehyde on the Rh (211) and (111) surfaces, chosen to describe steps and close-packed facets on catalyst particles. The model is based on DFT calculations using the BEEF-vdW functional. The mean-field kinetic model includes lateral adsorbate–adsorbate interactions, and the BEEF-vdW error estimation ensemble is used to propagate error from the DFT calculations to the predicted rates. The model shows the Rh(211) surface to be ~6 orders of magnitude more active than the Rh(111) surface, but highly selective toward methane, while the Rh(111) surface is intrinsically selective toward acetaldehyde. A variety of Rh/SiO 2 catalysts are synthesized, tested for catalytic oxygenate production, and characterized using TEM. The experimental results indicate that the Rh(111) surface is intrinsically selective toward acetaldehyde, and a strong inverse correlation between catalytic activity and oxygenate selectivity is observed. Furthermore, iron impurities are shown to play a key role in modulating the selectivity of Rh/SiO 2 catalysts toward ethanol. The experimental observations are consistent with the structure-sensitivity predicted from theory. As a result, this work provides an improved atomic-scale understanding and new insight into the mechanism, active site, and intrinsic selectivity of syngas conversion over rhodium catalysts and may also guide rational design of alloy catalysts made from more abundant elements.
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
A method to estimate the fractional fat volume within a ROI of a breast biopsy for WAXS applications: Animal tissue evaluation
Purpose: To develop a method to estimate the mean fractional volume of fat (ν{sup ¯}{sub fat}) within a region of interest (ROI) of a tissue sample for wide-angle x-ray scatter (WAXS) applications. A scatter signal from the ROI was obtained and use of ν{sup ¯}{sub fat} in a WAXS fat subtraction model provided a way to estimate the differential linear scattering coefficient μ{sub s} of the remaining fatless tissue. Methods: The efficacy of the method was tested using animal tissue from a local butcher shop. Formalin fixed samples, 5 mm in diameter 4 mm thick, were prepared. The two main tissue types were fat and meat (fibrous). Pure as well as composite samples consisting of a mixture of the two tissue types were analyzed. For the latter samples, ν{sub fat} for the tissue columns of interest were extracted from corresponding pixels in CCD digital x-ray images using a calibration curve. The means ν{sup ¯}{sub fat} were then calculated for use in a WAXS fat subtraction model. For the WAXS measurements, the samples were interrogated with a 2.7 mm diameter 50 kV beam and the 6° scattered photons were detected with a CdTe detector subtending a solid angle of 7.75 × 10{sup −5} sr. Using the scatter spectrum, an estimate of the incident spectrum, and a scatter model, μ{sub s} was determined for the tissue in the ROI. For the composite samples, a WAXS fat subtraction model was used to estimate the μ{sub s} of the fibrous tissue in the ROI. This signal was compared to μ{sub s} of fibrous tissue obtained using a pure fibrous sample. Results: For chicken and beef composites, ν{sup ¯}{sub fat}=0.33±0.05 and 0.32 ± 0.05, respectively. The subtractions of these fat components from the WAXS composite signals provided estimates of μ{sub s} for chicken and beef fibrous tissue. The differences between the estimates and μ{sub s} of fibrous obtained with a pure sample were calculated as a function of the momentum transfer x. A t-test showed that the mean of the differences did not vary from zero in a statistically significant way thereby validating the methods. Conclusions: The methodology to estimate ν{sup ¯}{sub fat} in a ROI of a tissue sample via CCD x-ray imaging was quantitatively accurate. The WAXS fat subtraction model allowed μ{sub s} of fibrous tissue to be obtained from a ROI which had some fat. The fat estimation method coupled with the WAXS models can be used to compare μ{sub s} coefficients of fibroglandular and cancerous breast tissue.
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
Activity of Cannabidiol on Ex Vivo Amino Acid Fermentation by Bovine Rumen Microbiota
Amino-acid-fermenting bacteria are wasteful organisms within the rumens of beef cattle that remove dietary amino nitrogen by producing ammonia, which is then excreted renally. There are currently no on-label uses for the control of this microbial guild, but off-label use of broad-spectrum antimicrobials has shown efficacy, which contributes to antimicrobial resistance. Plant-derived antimicrobials supplemented into the diets of cattle may offer worthwhile alternatives. This study sought to investigate the role of cannabidiol (CBD) as a terpenophenolic antimicrobial. Ex vivo cell suspensions were harvested from the rumen fluid of Angus × Holstein steers in non-selective media with amino acid substrates. The suspensions were treated with five concentrations of CBD (860 μg mL−1–0.086 μg mL−1) and incubated (24 h), after which ammonia production and viable number of cells per substrate and treatment were measured. The data demonstrated a ~10–15 mM reduction in ammonia produced at the highest concentration of CBD and negligible changes in the viable number of amino-acid-fermenting bacteria. CBD does not appear to be a biologically or economically viable terpenophenolic candidate for the control of amino acid fermentation in beef cattle.
Biotechnology & Applied Microbiology↗
Developing and Deploying Universal Diagnostic Platforms for One-Health Biosurveillance
ObjectiveOur goal is to develop deployable strategies for infectious disease diagnosis at the point-of-care that are applicable to multiple hosts of infection - conforming to the global One Health Strategy for diagnostics. We aim to develop methods that do not require prior knowledge of the pathogen in question, and can facilitate rapid and effective decision-making and situational awareness.IntroductionThere is an urgent need for diagnostic strategies for infections which are host-independent, so as to effectively track zoonotic spread, monitor animal carriers of pathogens, and evaluate transmission dynamics. Infection of a host - pathogen or human- by an animal results in recognition by the immune response, which consequently causes release of inflammatory mediators. Many scientists have explored the use of cytokines as diagnostic indicators of disease, but the conserved nature of the immune response in humans and animals results in cross-reactivity among many pathogens, making evaluation of the results difficult, especially in high disease burden populations. Measuring the pathogen-specific signature, however, is advantageous - as it offers discrete identification of active infection, and discrimination from exposure. It also offers a universal strategy that can be applied to human and animal hosts of infection - allowing for One Health Biosurveillance. Achieving this, however, requires the development of a) tailored strategies for the measurement of biochemically disparate pathogen signatures in clinical samples and b) ultra-sensitive detection of such signatures in the host. The sensor team at Los Alamos National Laboratory is working on both of these aspects, and the development of One Health Diagnostic platforms, the focus of the work presented here.MethodsMany of the biomarkers secreted by bacterial pathogens and recognized by innate immune receptors elicit host cytokine responses that are amphiphilic (largely glycolipids, lipoproteins or lipoglycans). Based on such, our team has developed tailored methods – membrane insertion and lipoprotein capture for the capture and detection of these greasy and stealthy molecules in infected blood. Sensitivity and specificity, assay optimization, alinearity associated with lipidic molecules and assay parameter development for biomarkers associated with bacterial pathogens will be presented. Considerations for clinical sampling for amphiphile detection in blood, and clinical study design will be demonstrated. Our team has developed an ultra-sensitive biosensor for detection of biomarkers in complex matrices based on the evanescent field properties of single mode planar optical waveguides. Detection of amphiphilic biomarkers in clinical samples, using the waveguide-based biosensor and tailored methods for the detection of such molecules has allowed for the diagnosis of infection in both human and animals hosts. Herein, we present two examples of the same; 1) diagnosis of tuberculosis in humans, cattle and badgers using pathogen-based diagnostic strategies; and 2) detection of Gram-negative Lipopolysacharides in beef and in patients with Salmonella-induced sepsis.ResultsWe have developed tailored ultra-sensitive waveguide-biosensor assays for the detection of Lipoarabinomannan (and other biomarkers) from Mycobacteria and demonstrated feasibility in blinded clinical studies in humans and cattle, demonstrating one -health compatibility.We have also demonstrated detection of lipopolysacharides from eight different serotypes of Shiga-toxin carrying E. coli in beef, and Salmonella Typhimurium in pediatric patients using the same approach.ConclusionsWe have demonstrated clinical feasibility of a One Health Strategy for point-of-care diagnostics of bacterial infections in blood for tuberculosis and Gram-negative pathogens in clinical samples. The results will be demonstrated with several discussion points on the consideration of unconventional biochemistry of pathogens for diagnostics, factors influencing point-of-care deployment and integration of diagnostic platforms for biosurveillance. One Health Considerations and challenges therein will also merit discussion.ReferencesSakamuri, R; et al. Accurate Tracking of Bovine Tuberculosis Biomarkers in Infected Cattle using a Novel Biomarker Capture Strategy, Analytical Sciences, 33(4): 457-460, 2017.Noormohammed A et al. Detection of Salmonella LPS in Patient blood by Lipoprotein Capture. Proc. SPIE 10072, Optical Diagnostics and Sensing XVII: Toward Point-of-Care Diagnostics, 100720A , 2017. doi:10.1117/12.2253506Stromberg L et al. Membrane Insertion for the Detection of Lipopolysaccharides: Exploring the Dynamics of Amphiphile-in-Lipid Assays, PloS One, April 2016.Sakamuri RM et al. Association of lipoarabinomannan with high density lipoprotein in blood: implications for diagnostics.Tuberculosis (Edinb). 2013 May;93(3):301-7. doi: 10.1016/j.tube.2013.02.015. Epub 2013 Mar 16Mukundan H et al. Understanding the interaction of Lipoarabinomannan with membrane mimetic architectures. Tuberculosis, 92(1) 32-47, 2012.Mukundan H et al., Waveguide-based Sensors for Pathogen Detection, Invited Review, Sensors, 9(7), 5783-5809.
Mukundan, Harshini↗
Preserving Perishables (Dormavac)
A new commercial product that can preserve perishable commodities for weeks without freezing, so that they can be shipped fresh without the cost of air freight, has been developed by Grumman Corporation, Bethpage, Long Island, New York. The development benefited from the company's experience in developing the environmental control system for the Lunar Module, which delivered Apollo astronauts to the surface of the moon. Called Dormavac, the system provides a commodity-preserving environment within an aluminum container that can be transported by truck, rail or ship. Dormavac creates a cold-but above freezing-environment with high relative humidity and very low air pressure. The saturated air minimizes commodity weight loss and the air is automatically changed several times an hour to flush away odors and harmful gases released by the commodities. According to company literature, Dormavac significantly extends the transportation life of perishables. For example, pork has a normal cold storage life of about seven days, beef two weeks and tomatoes three weeks; with Dormavac, pork remains fresh for three weeks, beef more than six weeks and tomatoes seven weeks or more. Dormavac is manufactured and marketed by Grumman Allied Industries, Woodbury, New York. In developing the system, Grumman Allied drew upon the technological resources of another company subsidiary, Grumman Aerospace. Engineers who had earlier worked on Lunar Module environmental control brought their know-how and experience to the Dormavac development.
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