SEARCH · Search NASA
Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.
Measurements of CH4 uptake and N2O emissions in native, nitrogen-fertilized, and wheat-growing prairie soils from spring to late autumn, 1990 are reported. It is found that nitrogen fertilization and cultivation can both decrease CH4 uptake and increase N2O production, thereby contributing to the increasing atmospheric concentrations of these gases.
The application of bioregenerative life support systems provides an attractive approach to minimize resupply requirement and ultimate self-sufficiency on long duration manned missions in space. The on-board cultivation of salad-type vegetables for crew consumption has been proposed as a first step approach towards reducing a total reliance on the resupply of food. The recent advances in the development of space flight plant growth facilities such as the Plant Generic Bioprocessing Apparatus (PGBA) have established a firm technical basis upon which the implementation of a 'salad machine' concept may be achieved. A presentation on ground based studies will be made evaluating (a) the operational performance of the PGBA facility in a crop production mode and (b) the qualitative and quantitative value of salad plant material produced within the chamber.
The prototype of a miniaturized cell cultivation instrument for animal cell culture experiments aboard Spacelab is presented (Dynamic cell culture system: DCCS). The cell chamber is completely filled and has a working volume of 200 microliters. Medium exchange is achieved with a self-powered osmotic pump (flowrate 1 microliter h-1). The reservoir volume of culture medium is 230 microliters. The system is neither mechanically stirred nor equipped with sensors. Hamster kidney (Hak) cells growing on Cytodex 3 microcarriers were used to test the biological performance of the DCCS. Growth characteristics in the DCCS, as judged by maximal cell density, glucose consumption, lactic acid secretion and pH, were similar to those in cell culture tubes.
Plantlets of daylily (Hemerocallis cv. Autumn Blaze) regenerated from cell suspensions, and 4 clonal populations of Haplopappus gracilis were aseptically cultivated aboard the Shuttle "Discovery" during a 5-day mission within NASA's Plant Growth Unit (PGU) apparatus. Daylily was selected as a representative herbaceous perennial monocotyledon and the haplopappus clones represented an annual dicotyledon. The latter included 4 strains with different physiological and morphological characteristics: two aseptic seedling clones (each generated from a single seedling) and two tissue culture-derived lines. Mean daily growth rates for the primary shoots of all plantlets averaged 4.13 mm day-1 (SD = 2.20) for the flight experiment and 4.68 mm day-1 (SD = 2.59) for the ground control. Comparable growth rates calculated by summing both the primary and secondary shoots for all plantlets were 5.94 mm day-1 (SD = 2.89) for the flight experiment and 6.38 mm day-1 (SD = 3.71) for the control. Statistically significant differences existed between: (1) flight vs control primary shoot growth (the controls growing more than plantlets subjected to spaceflight conditions), (2) the different populations (the daylily gaining more shoot material than any of the haplopappus populations and the haplopappus seedling clones outperforming the tissue culture-derived haplopappus lines), and (3) the individual Plant Growth Chambers contained within the PGU. The data suggest that some spaceflight-associated factor(s) increased the tendency for primary shoot apices to degrade or senesce, resulting in the release of apical dominance and permitting the emergence of axillary branches, which subsequently partially compensated for the reduced primary axis growth. In addition to spaceflight-associated factors, the physiologically diverse nature of the experimental material as well as environmental heterogeneities within the culture apparatus contributed to the variation in growth results. The findings could explain some discrepancies reported from various plant culture experiments conducted in space.
The complex polar lipids of the hot spring cyanobacterial mat in the 50 to 55 degrees C region of Octopus Spring, Yellowstone National Park, and of thermophilic bacteria cultivated from this or similar habitats, were compared in an attempt to understand the microbial sources of the major lipid biomarkers in this community. Intact complex lipids were analyzed directly by fast atom bombardment mass spectrometry (FAB-MS), two-dimensional thin-layer chromatography (TLC), and combined TLC-FAB-MS. FAB-MS and TLC gave qualitatively similar results, suggesting that the mat contains major lipids most like those of the cyanobacterial isolate we studied, Synechococcus sp. strain Y-7c-s. These include monoglycosyl, diglycosyl, and sulfoquinosovyl diglycerides (MG, DG, and SQ, respectively) and phosphatidyl glycerol (PG). Though Chloroflexus aurantiacus also contains MG, DG, and PG, the fatty acid chain lengths of mat MGs, DGs, and PGs resemble more those of cyanobacterial than green nonsulfur bacterial lipids. FAB-MS spectra of the lipids of nonphototrophic bacterial isolates were distinctively different from those of the mat and phototrophic isolates. The lipids of these nonphototrophic isolates were not detected in the mat, but most could be detected when added to mat samples. The mat also contains major glycolipids and aminophospholipids of unknown structure and origin. FAB-MS and TLC did not always give quantitatively similar results. In particular, PG and SQ may give disproportionately high FAB-MS responses.
Oligodeoxynucleotide hybridization probes were developed to complement specific regions of the small subunit (SSU) rRNA sequences of cultivated and uncultivated cyanobacteria and Chloroflexus-like bacteria, which inhabit hot spring microbial mats. The probes were used to investigate the natural distribution of SSU rRNAs from these species in mats of Yellowstone hot springs of different temperatures and pHs as well as changes in SSU rRNA distribution resulting from 1-week in situ shifts in temperature, pH, and light intensity. Synechococcus lividus Y-7c-s SSU rRNA was detected only in the mat of a slightly acid spring, from which it may have been initially isolated, or when samples from a more alkaline spring were incubated in the more acid spring. Chloroflexus aurantiacus Y-400-fl SSU rRNA was detected only in a high-temperature mat sample from the alkaline Octopus Spring or when lower-temperature samples from this mat were incubated at the high-temperature site. SSU rRNAs of uncultivated species were more widely distributed. Temperature distributions and responses to in situ temperature shifts suggested that some of the uncultivated cyanobacteria might be adapted to high-, moderate-, and low-temperature ranges whereas an uncultivated Chloroflexus-like bacterium appears to have broad temperature tolerance. SSU rRNAs of all uncultivated species inhabiting a 48 to 51 degrees C Octopus Spring mat site were most abundant in the upper 1 mm and were not detected below a 2.5-to 3.5-mm depth, a finding consistent with their possible phototrophic nature. However, the effects of light intensity reduction on these SSU rRNAs were variable, indicating the difficulty of demonstrating a phototrophic phenotype in light reduction experiments.
Goals of the 3-month experiment GREENHOUSE using the equipment of greenhouse SVET (ECO-PSY-95) were to feature growth and development of wheat through the entire cycle of ontogeny under the maximally mimicked MIR environment, and to try out the procedures and timeline of space experiment GREENHOUSE-2 as a part of the fundamental biology investigations within the MIR/NASA space science program. Irradiation intensity (PAR) was 65 W/m2 and 38 W/m2 in the experiment and laboratory control, respectively. Values of other environmental parameters were MIR average (18-25 degrees C, relative air humidity in the interval between 40% and 75%, total gas pressure of about 660 to 860 mm Hg, partial oxygen pressure within the range from 140 to 200 mm Hg, partial carbon dioxide pressure up to 7 mm Hg). Experimental results showed that wheat cultivation in inhabited chamber under a modified lighting unit providing greater irradiation of the crop area produced more plant mass although seed production dropped. Low grain content in ears could be the aftermath of the gaseous trace contaminants in the chamber atmosphere.
The development of a spaceflight-rated Porous Tube Insert Module (PTIM) nutrient delivery tray has facilitated a series of studies evaluating various aspects of water and nutrient delivery to plants as they would be cultivated in space. We report here on our first experiment using the PTIM with a software-driven feedback moisture sensor control strategy for maintaining root zone wetness level set-points. One-day-old wheat seedlings (Tritium aestivum cv Apogee; N=15) were inserted into each of three Substrate Compartments (SCs) pre-packed with 0.25-1 . mm Profile(TradeMark) substrate and maintained at root zone relative water content levels of 70, 80 and 90%. The SCs contained a bottom-situated porous tube around which a capillary mat was wrapped. Three Porous Tubes. were planted using similar protocols (but without the substrate) and also maintained at these three moisture level set-points. Half-strength modified Hoagland's nutrient solution was used to supply water and nutrients. Results on hardware performance, water usage rates and wheat developmental differences between the different experimental treatments are presented.
Nature’s Fynd, a food company headquartered in Chicago, Illinois, partnered with Montana State University on NASA STTR contracts to further develop a micro-gravity biofilm-biomat reactor, which cultivates a unique fungus to form a dense protein material. The resulting “biomat” could serve as a nutritious food source for life away from Earth. Nature’s Fynd has received external investments totaling more than $500 million for developing its technologies, and the company recently launched its meatless and dairy-free foods in specific retailers.
The decadal survey will help guide the Heliophysics community to create opportunities for future success. A uniquely fundamental question will drive science innovations and discoveries in the coming decades: What research environment and community will we build? The most innovative scientific ideas and discoveries develop in safe, inclusive, diverse, accessible, and collaborative environments. These environments strengthen all types of collaborations and advance innovations in concepts and applications. If we ignore this critical aspect of science, current issues regarding diversity, retention, and succession will persist. This paper discusses current critical problems and introduces actionable steps that can cultivate a culture of inclusivity.
Plant feeding by air culture method for closed system
Optimum environmental conditions steady state growth of hydrogen fixing bacteria cultures
Environment effects on growth of Hydrogenomonas eutropha in dense culture
There are no author-identified significant results in this report.
There are no author-identified significant results in this report.
There are no author-identified significant results in this report.
The maintainance of actively secreting human pituitary growth hormone cells (somatotrophs) in vitro was studied. The primary approach was the testing of agents which may be expected to increase the release of the human growth hormone (hGH). A procedure for tissue procurement is described along with the methodologies used to dissociate human pituitary tissue (obtained either at autopsy or surgery) into single cell suspensions. The validity of the Biogel cell column perfusion system for studying the dynamics of GH release was developed and documented using a rat pituitary cell system.
There are no author-identified significant results in this report.